BMC Research Notes最新文献

{"title":"Candidal speciation and carriage in oral cavity of HIV-positive children/adults and healthy individuals in the South Canara district of India: a comparative study.","authors":"Sneha Ks, Srikant Natarajan, Karen Boaz, Shrikala Baliga, John Ramapuram, Monica Charlotte Solomon, Nidhi Manaktala, Nunna Sai Chitra","doi":"10.1186/s13104-025-07190-7","DOIUrl":"10.1186/s13104-025-07190-7","url":null,"abstract":"<p><strong>Objective: </strong>Candidiasis, an opportunistic infection that is prevalent in HIV-positive children and adults, is caused by various Candida species, Candida albicans along with certain non-albicans Candida species. The prevalence of these species varies across age groups due to multiple factors. Identification of these species becomes necessary as any antifungal therapy requires species-specific targeting to manage candidiasis effectively. With this background, the present study aimed to evaluate the prevalence and speciation of Candida in HIV-positive children (n = 30) and adults (n = 40) and to compare these findings with those in healthy individuals. Ten mL saliva was collected from HIV-positive and HIV-negative (healthy) patients and cultured on CHROMAgar™.</p><p><strong>Results: </strong>The proportion and quantity of candidal colonization was higher in HIV-positive children (93.30%) as compared to adults (67.50%). An increased profile of Nakaseomyces glabrata (previously Candida glabrata) was seen in children while the adults showed increase in colonization of C. tropicalis. The shift in profiles of species from Candida albicans to 'non-albicans' Candida species is of clinical relevance as it directly impacts on the antimicrobial efficacy of chosen anti-fungal agents.</p>","PeriodicalId":9234,"journal":{"name":"BMC Research Notes","volume":"18 1","pages":"130"},"PeriodicalIF":1.6,"publicationDate":"2025-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11948835/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143728635","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Understanding the educational needs of undergraduate nursing students regarding end-of-life care: a qualitative content analysis.","authors":"Zahra Taheri-Ezbarami, Fateme Jafaraghaee, Ali Karimian Sighlani, Seyed Kazem Mousavi","doi":"10.1186/s13104-025-07180-9","DOIUrl":"10.1186/s13104-025-07180-9","url":null,"abstract":"<p><strong>Background: </strong>Although nurses have a fundamental role in end-of-life care, nursing curricula have not paid enough attention to this area. This research aimed to understand the educational needs of undergraduate nursing students regarding end-of-life care.</p><p><strong>Methods: </strong>This study was a qualitative content analysis conducted in 2023 in which 16 participants, including faculty members, nurses, head nurses, nursing educational supervisors, nursing service managers, PhD and MSc students, were selected purposefully. Individual in-depth semi-structured interviews were used face-to-face and over the phone to collect information. The data was analyzed using Elo and Kyngas' inductive content analysis approach with the help of MAXQDA 2020 software. Also, Elo et al.'s checklist was used to check the rigor of the data.</p><p><strong>Results: </strong>After coding the interviews, 773 initial codes were generated, which were reduced to 679 after several revisions. These codes were placed into 46 subcategories, 17 categories, and six themes, including the principles of end-of-life care, physical care, psycho-social care, spiritual care, ethical challenges, and after-death care.</p><p><strong>Conclusion: </strong>This study determined the educational needs of undergraduate nursing students regarding end-of-life care. Therefore, it is suggested that the results of this study be considered when designing related educational programs for nursing students and nurses.</p>","PeriodicalId":9234,"journal":{"name":"BMC Research Notes","volume":"18 1","pages":"129"},"PeriodicalIF":1.6,"publicationDate":"2025-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11948791/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143717797","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Situational judgment test in pharmacy education: assessing professionalism capability among students.","authors":"Narges Fatehi-Firouzabadi, Fatemeh Keshmiri","doi":"10.1186/s13104-025-07183-6","DOIUrl":"10.1186/s13104-025-07183-6","url":null,"abstract":"<p><strong>Objective: </strong>Situational Judgment tests are recognized as a valid predictor of job performance. In the present study, the students' professionalism capabilities were investigated using the situational judgment test. This cross-sectional study was conducted in 2023-2024. The situational judgment test includes four scenarios in the faculty situation (classroom) and six scenarios in the workplace situation in the field (hospital and urban pharmacy) with the format of \"selected-response format\" examined. 160 pharmacy students in different academic years participated. The cut-off scorer of situational judgment test was determined using the Cohen method and the norm-reference method of standard-setting.</p><p><strong>Results: </strong>The results showed that the scores of students in the situational judgment test were reported as 14.13 ± 6.07. According to Cohen's method, the score of 60 pharmacy students (50%) is lower than the cut-off score. The cut-off score based on the norm-reference method of standard-setting showed that 33 students (20.6%) scored below the cut-off score. The results showed that the students who participated in the professionalism course scored an average of 2.62 higher than the students who did not participate in these training courses. (p-value = 0.015). The development of professional education as a longitudinal program in the pharmacy curriculum is suggested.</p>","PeriodicalId":9234,"journal":{"name":"BMC Research Notes","volume":"18 1","pages":"128"},"PeriodicalIF":1.6,"publicationDate":"2025-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11948762/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143717796","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparing the effect of using calcified autogenous nano dentin particles versus micro dentin particles in the healing of mandibular bony defects in New Zealand rabbits.","authors":"Sarah Yasser, Altaib Abd Al Razik Mohammed, Samy El-Safty, Ahmed Shon, Redhwan Saleh Al-Gabri, Ahmed Yaseen Alqutaibi, Hasnaa Fouad, Reda G Saleh","doi":"10.1186/s13104-025-07191-6","DOIUrl":"10.1186/s13104-025-07191-6","url":null,"abstract":"<p><strong>Objective: </strong>This study aimed to compare the regenerative effect of autogenous micro-dentin and nano-dentin particles on bone regeneration in rabbits' mandibular defects. Sixty adult New Zealand rabbits were randomly divided into three groups: a control group, a micro-dentin group, and a nano-dentin group. A critical-sized bony defect was created at the lower border of the mandible. Bone regeneration was evaluated at two, four, and eight weeks using light microscopy, cone beam computed tomography (CBCT) scans, and histomorphometric analysis.</p><p><strong>Results: </strong>Nano-dentin significantly enhanced bone density and defect closure, as evidenced by CBCT and histological analyses. At eight weeks, it promoted extensive new bone formation, nearly bridging the defect, with minimal residual graft material compared to the micro-dentin group. Histomorphometric analysis confirmed its superior osteogenic potential, demonstrating enhanced bone regeneration and graft resorption. These findings highlight nano-dentin as a highly effective biomaterial for mandibular bone repair.</p>","PeriodicalId":9234,"journal":{"name":"BMC Research Notes","volume":"18 1","pages":"125"},"PeriodicalIF":1.6,"publicationDate":"2025-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11934702/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143708618","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dataset of obesity in relation to female-specific cancers in middle eastern countries, 1990 to 2016.","authors":"Mojtaba Daneshvar","doi":"10.1186/s13104-025-07187-2","DOIUrl":"10.1186/s13104-025-07187-2","url":null,"abstract":"<p><strong>Objectives: </strong>Understanding the relationship between obesity and female-specific cancers (FSCs) is crucial for public health planning and policy development. The current data paper presented a dataset that includes obesity prevalence and incidence rates of breast, ovarian, cervical, and uterine cancers among women in Middle Eastern countries. This dataset could be used for time-trend analysis and different forecasting models. Moreover, exploring the relationship between obesity and FSCs is important to develop preventive healthcare services, especially among developing countries.</p><p><strong>Data description: </strong>The dataset comprises official statistics obtained from reputable sources including the world bank and global burden of disease (GBD) database. The data include a total of 405 observations across 15 middle-eastern countries, from 1990 to 2016. Key variables are obesity prevalence and incidence rate of four major cancers in women including breast cancer, ovarian cancer, cervical cancer, and uterine cancer. This panel data is mainly prepared to investigate the temporal relationship between obesity prevalence and FSC incidence rates, and also performing Counterfactual analysis. Moreover, this data could be utilized for advanced machine learning techniques to estimate future shifts in trend and patterns over time.</p>","PeriodicalId":9234,"journal":{"name":"BMC Research Notes","volume":"18 1","pages":"124"},"PeriodicalIF":1.6,"publicationDate":"2025-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11934536/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143708620","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Failure to prevent classical scrapie after repeated decontamination of a barn.","authors":"Timm Konold, John Spiropoulos, Peter Bellerby, Hugh A Simmons","doi":"10.1186/s13104-025-07188-1","DOIUrl":"10.1186/s13104-025-07188-1","url":null,"abstract":"<p><strong>Objective: </strong>Prions, the causative agent of scrapie in sheep, are extremely resistant to disinfection and can remain biologically active for years, which makes it challenging to prevent re-infection of susceptible animals on farms after a scrapie outbreak. The present study investigated the effectiveness of disinfection of a barn that previously housed scrapie-affected sheep as part of the husbandry of scrapie infected sheep on the farm. The barn was decontaminated with sodium hypochlorite for four times the recommended exposure time. Two cohorts, consisting of 25 and 21 sheep, with susceptible prion protein genotypes (VRQ/VRQ), born 2 years apart, were housed in the barn and infection monitored by examination of rectal biopsies.</p><p><strong>Results: </strong>One sheep from the first cohort and four from the second were found to be infected from 775 (first cohort) and 550 days (second cohort) post exposure. It is concluded that decontamination with sodium hypochlorite at the recommended concentration and longer exposure time did not prevent re-infection of susceptible sheep. Disinfection of contaminated premises to eradicate scrapie continues to be a challenge.</p>","PeriodicalId":9234,"journal":{"name":"BMC Research Notes","volume":"18 1","pages":"126"},"PeriodicalIF":1.6,"publicationDate":"2025-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11938569/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143708545","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Behavioral and metabolic effects of escapable electric foot shock stress in male mice.","authors":"Vitalii Balatskyi, Olha Strilbytska, Oleksandra Abrat, Anastasiia Tkachyk, Maria Lylyk, Volodymyr Lushchak, Maria Bayliak","doi":"10.1186/s13104-025-07189-0","DOIUrl":"10.1186/s13104-025-07189-0","url":null,"abstract":"<p><strong>Background: </strong>The pathophysiology of post-traumatic stress disorder (PTSD) is not fully understood, prompting research using animal models. One of the approaches to induce PTSD-like traits in rodents involves exposure to unavoidable, unpredictable electric foot shocks. This study explored the effects of repeated electric foot shocks on behavior, hematology, and metabolic parameters in male C57BL/6J mice. The mice were subjected to 15 electrical stimuli (each 0.8 mA for 10 s, 10 s interval between sessions) in an electric foot shock apparatus featuring metal floor bars and two insulating bars for two consecutive days.</p><p><strong>Results: </strong>Stressed mice showed enhanced memory retention of fear, as evidenced by an increased number of freezing events in the aversive context test. In the elevated plus maze test, stressed mice spent significantly less time in the open arms, indicating anxious behavior. However, no significant behavioral differences were observed between the stressed and control groups in the open field test. Stressed mice had a larger hypothalamic mass, elevated liver lipid peroxide levels, and a higher red blood cell count, with no changes in total leukocyte count. Thus, escapable foot shock induces fear response, anxiety-like behavior and minor metabolic changes in mice.</p>","PeriodicalId":9234,"journal":{"name":"BMC Research Notes","volume":"18 1","pages":"127"},"PeriodicalIF":1.6,"publicationDate":"2025-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11938689/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143708615","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The participation in cervical cancer screening is not altered by the Hawthorne effect among patients of doctors participating in the randomized clinical trial PaCUDAHL.","authors":"Gabrielle Lisembard, Michaël Rochoy, François Quersin, Valérie Deken, Alain Duhamel, Axel Descamps, Christophe Berkhout, Fanny Serman","doi":"10.1186/s13104-025-07186-3","DOIUrl":"10.1186/s13104-025-07186-3","url":null,"abstract":"<p><strong>Objective: </strong>The PaCUDAHL randomized clinical trial evaluated an HPV self-sampling device provided by the family doctor to female patients not participating in the usual opportunistic cervical screening program from 2016 to 2019. Reliable data on the Hawthorne (observer) effect (HE) in clinical trials were lacking. This nested study aimed to verify whether there was a significant difference between participating and non-participating general practitioners (GPs) in the trial, and to measure whether there was an HE in the female patients of participating GPs.</p><p><strong>Results: </strong>We carried out an analytical retrospective cohort study involving 332 GPs and their 70,983 female patients, aged 25-65, registered with the Health Insurance Fund of Flanders, using claims database for the three-year periods 2012-2015 and 2016-2019. Statistical analyses were performed using a linear generalized hierarchical mixed model with geographic level as a random effect. The patients of the 24 participating GPs did not have a cervical cancer screening rate different from that of the non-participating GPs, either before recruitment (p = 0.24) or during the PaCUDAHL trial period (p = 0.15). There were significant increases in cervical cancer screening rates over four years regardless of the group considered (p < 0.0001). In conclusion there was no observer effect but a significant cohort effect.</p>","PeriodicalId":9234,"journal":{"name":"BMC Research Notes","volume":"18 1","pages":"123"},"PeriodicalIF":1.6,"publicationDate":"2025-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11934682/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143699726","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of an improved construct for spectinomycin selection in plant transformation.","authors":"Kent F McCue, Min Shao, Jennie Huynh, Tai Minh, Mandy Chan, York Moy, James G Thomson","doi":"10.1186/s13104-025-07178-3","DOIUrl":"10.1186/s13104-025-07178-3","url":null,"abstract":"<p><p>Efficient selection of transgenic plants is essential in plant biotechnology, and development of effective selectable marker systems plays a crucial role, especially as multiple rounds of transformation and gene stacking may require multiple selectable markers. The present study aims to develop and evaluate an enhanced spectinomycin resistance construct to provide robust selection for transformation events. The new construct was compared in multiple plant species to the standard spectinomycin selectable marker gene currently used for soybean transformation and to the neomycin phosphotransferase (nptII) gene that confers kanamycin resistance. In Arabidopsis thaliana, potato, and citrus, the transformation efficiency provided by the enhanced spectinomycin marker was approximately equal to that of kanamycin and considerably better than that observed for the standard spectinomycin marker. In soybean, the enhanced spectinomycin resistance marker preformed as well as the standard spectinomycin gene. These results demonstrate that the enhanced spectinomycin construct provides strong resistance, comparable to kanamycin in multiple species, offering an alternative tool for transgenic plant selection.</p>","PeriodicalId":9234,"journal":{"name":"BMC Research Notes","volume":"18 1","pages":"122"},"PeriodicalIF":1.6,"publicationDate":"2025-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11929352/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143691268","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Role of smart phones in improving psychological well-being and successful ageing of Iranian old women living with Technophobia: a randomized controlled trial.","authors":"Arezoo Avazzadeh, Odai Y Khasawneh, Samuel T Faloye, Abdolrahim Asadollahi, Mahin Nazari","doi":"10.1186/s13104-025-07181-8","DOIUrl":"10.1186/s13104-025-07181-8","url":null,"abstract":"<p><strong>Background: </strong>Covid-19 pandemic and its following lock-down lead to loneliness of older adults especially females. Smart phones can reduce the crises-caused complications. Technophobia is an overwhelming fear of technology or the powers of artificial intelligence.</p><p><strong>Aims: </strong>The study focused on determining the impact of regular smartphone use in decreasing Technophobia and improving mental health and successful ageing (SA) for older adults residing alone during the Covid-19 pandemic.</p><p><strong>Materials & methods: </strong>In this randomized controlled trial, 80 old females living alone as the member of a daily caring center were randomly categorized into intervention (40 people) and control (40 people) groups. The intervention group received 9 skill training online sessions of using smart phone through WhatsApp. The data were collected before and two months after intervention by demographic, Technophobia, Ryff's Psychological Wellbeing (PW), and Successful Ageing Index. The data were analyzed by SPSS (V.28) through independent and paired t-tests and effect size measures.</p><p><strong>Results: </strong>The results showed negative (r = 0.303) and significant (P < 0.05) correlation between Technophobia and PW of old women living alone. Also, there were negative (r = 0.644) and significant (P < 0.001) correlation between Technophobia and SA. The findings revealed no significant differences between the two studied groups based on Technophobia (r = 0.553), PW (P > 0.05) and SA (P > 0.05). However, there were significant differences between the two groups regarding the mean scores of Technophobia, PW, and SA in the two groups (P < 0.001).</p><p><strong>Discussion & conclusion: </strong>Online training of smart phone use is proposed to effectively reduce Technophobia and improve PW and successful ageing. Clinical Trials Registration IRCT20180514039648N2, Registration Date: 25 July 2023.</p>","PeriodicalId":9234,"journal":{"name":"BMC Research Notes","volume":"18 1","pages":"121"},"PeriodicalIF":1.6,"publicationDate":"2025-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11929283/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143676841","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}