BMC Research Notes最新文献

{"title":"Assessment of the effect of culture components of clinical Pseudomonas aeruginosa isolates on vancomycin eradication of Staphylococcus aureus mature biofilms.","authors":"Mona Farag, Abeer Ghazal, Gamaleldin Elsawaf, Ghada Abu-Sheasha, Aliaa Aboulela","doi":"10.1186/s13104-026-07718-5","DOIUrl":"10.1186/s13104-026-07718-5","url":null,"abstract":"","PeriodicalId":9234,"journal":{"name":"BMC Research Notes","volume":" ","pages":""},"PeriodicalIF":1.7,"publicationDate":"2026-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13063584/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147430970","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of the impact of malaria and erythrocyte G6PD deficiency on anaemia outcomes among children in Jos Northcentral Nigeria.","authors":"Caroline Amuche Okoli, Deborah Isaiah Wamu, Favour Deffi, Philip Dakwar Leshak","doi":"10.1186/s13104-026-07658-0","DOIUrl":"10.1186/s13104-026-07658-0","url":null,"abstract":"","PeriodicalId":9234,"journal":{"name":"BMC Research Notes","volume":" ","pages":""},"PeriodicalIF":1.7,"publicationDate":"2026-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13088677/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147431028","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Targeted native long-read sequencing of DNA methylation alterations following CRISPR-Cas9-induced double-strand breaks in human cells.","authors":"Yingzi Zhang, Mengge Wang, Chongwei Bi, Mo Li","doi":"10.1186/s13104-026-07761-2","DOIUrl":"10.1186/s13104-026-07761-2","url":null,"abstract":"<p><strong>Objectives: </strong>CRISPR-Cas9 nucleases are widely used to introduce targeted DNA double-strand breaks (DSBs) for genome engineering, but the long-term impact of these lesions on local epigenetic information remains poorly characterized. In a companion research article, we used Cas9-assisted targeted nanopore sequencing (CTS) to reveal that CRISPR-Cas9-induced DSBs can disrupt local epigenetic maintenance across multiple genomic contexts and cell systems. Here, we present a structured description of the raw and minimally processed datasets underlying the study. These datasets provide base-resolution measurements of 5-methylcytosine (5mC) and 5-hydroxymethylcytosine (5hmC) at the differentially methylated regions (DMRs) of several imprinted loci, two heterochromatic regions, a cancer-associated promoter epimutation region, and the SNRPN DMR at early/late passages of a clonal line. They enable re-analysis and methodological benchmarking of DSB-associated epigenetic instability.</p><p><strong>Data description: </strong>We provide aligned BAM files and per-CpG methylation calls for multiple genomic contexts under both CRISPR-targeted and non-targeting control conditions. Specifically, the collection includes: (i) imprinted loci in human embryonic stem cells (hESCs), including small nuclear ribonucleoprotein polypeptide N (SNRPN), paternally expressed 10 (PEG10), and KCNQ1 opposite strand/antisense transcript 1 (KCNQ1OT1), (ii) heterochromatic regions in hESCs, including urothelial cancer associated 1 (UCA1), and cysteine rich C-terminal 1 (CRCT1)), (iii) the epimutation locus of MutL homolog 1 (MLH1) in RKO cells, and (iv) the DMR of SNRPN locus in early- and late-passage derivatives of a single hESC clone. For each collection, there is a dataset that includes both the raw aligned Nanopore sequencing reads (BAM) deposited in the NCBI Sequence Read Archive (SRA) and the corresponding processed per-CpG 5mC/5hmC matrices deposited in Zenodo. All higher-level analyses in the research article-such as DMR calling, haplotype-resolved analyses, and structural variant (SV) characterization-are fully reproducible using these deposited data. Additional processed analyses are comprehensively documented in the companion article and are therefore not duplicated here. Together, these datasets offer a rich resource for benchmarking long-read methylation analysis workflows and further investigation of DSB-associated epigenetic instability across diverse genomic contexts.</p>","PeriodicalId":9234,"journal":{"name":"BMC Research Notes","volume":" ","pages":""},"PeriodicalIF":1.7,"publicationDate":"2026-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13088674/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147430987","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring non-compliance with traffic laws and its role in road crashes in Yazd: a qualitative content analysis.","authors":"Maryam Ghavami, SeyedSaeed MazloomyMahmoodabad, Shakiba Zahed","doi":"10.1186/s13104-026-07732-7","DOIUrl":"10.1186/s13104-026-07732-7","url":null,"abstract":"","PeriodicalId":9234,"journal":{"name":"BMC Research Notes","volume":" ","pages":""},"PeriodicalIF":1.7,"publicationDate":"2026-03-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13085580/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147389402","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Factors related to supplementation use and self-reported vitamin deficiency in the palestinian society.","authors":"Nihal Natour, Sajeda Hamadi, Wafaa Najar, Ahmed Abudawwas, Saja Elewi","doi":"10.1186/s13104-026-07759-w","DOIUrl":"10.1186/s13104-026-07759-w","url":null,"abstract":"","PeriodicalId":9234,"journal":{"name":"BMC Research Notes","volume":" ","pages":""},"PeriodicalIF":1.7,"publicationDate":"2026-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13081240/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147372564","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Standardization of C6/36 cells for DENV-2 virological assays: isolation, TCID₅₀ and serum neutralization.","authors":"Elmo José Dos Santos, Amanda da Rocha Santos, Mayanna Moreira Costa Fogaça, Naiara Soares da Silva, Milena Silva Souza, Ruth Dálety da Silva Brito, Paloma Oliveira Vidal, Samuel Santos Pereira, Jéssica Pires Farias, Alexander Birbrair, Rúbens Prince Dos Santos Alves, Wilson Barros Luiz, Luís Carlos de Souza Ferreira, Jaime Henrique Amorim","doi":"10.1186/s13104-026-07756-z","DOIUrl":"10.1186/s13104-026-07756-z","url":null,"abstract":"","PeriodicalId":9234,"journal":{"name":"BMC Research Notes","volume":" ","pages":""},"PeriodicalIF":1.7,"publicationDate":"2026-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13077881/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147364237","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Probiotic Lactobacillus rhamnosus GKLC1 enhances endurance and metabolism in mice with low exercise capacity.","authors":"You-Shan Tsai, Yi-Ju Hsu, Chi-Chang Huang, Shih-Wei Lin, Yen-Lien Chen, Chin-Chu Chen","doi":"10.1186/s13104-026-07757-y","DOIUrl":"10.1186/s13104-026-07757-y","url":null,"abstract":"","PeriodicalId":9234,"journal":{"name":"BMC Research Notes","volume":" ","pages":""},"PeriodicalIF":1.7,"publicationDate":"2026-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13077920/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147369059","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Outcomes in patients with cancer following opioid initiation in Japan: an exploratory study.","authors":"Takaomi Kessoku, Takahiro Higashibata, Shunsuke Oyamada, Yasuhide Morioka, Yuichi Koretaka, Yasushi Ichikawa, Atsushi Nakajima, Takayuki Hisanaga","doi":"10.1186/s13104-026-07752-3","DOIUrl":"10.1186/s13104-026-07752-3","url":null,"abstract":"","PeriodicalId":9234,"journal":{"name":"BMC Research Notes","volume":" ","pages":""},"PeriodicalIF":1.7,"publicationDate":"2026-03-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13069800/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147353864","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"New insight on the assessment of potassium silicate on pharmaceutical, morphological, and elemental status of Ficus carica: in vitro.","authors":"Nesreen Houssien Abou-Baker, Doha H Aboubaker, Nabil S Mustafa, Rania A Taha","doi":"10.1186/s13104-026-07671-3","DOIUrl":"10.1186/s13104-026-07671-3","url":null,"abstract":"<p><strong>Background and objective: </strong>Recently, silicon (Si) has been demonstrated to be an important element in agriculture. Its significance in plant growth has prompted numerous studies. However, the knowledge about its role in plant tissue cultures is still limited. In an attempt to understand the role and effect of silicon on plant growth, morphologically and physiologically, in vitro, regenerated fig (Ficus carica var. Sultany) was used to study this purpose.</p><p><strong>Materials and methods: </strong>Silicon as potassium silicate (K₂O.4SiO₂; PS) was added to the MS medium at 0 (PS0), 0.1 (PS1), 1.0 (PS2), and 10.0 (PS3) mg L^- 1. After four subcultures, morphological measurements and contents of element compounds, including N, P, K, Na, Ca, Fe, Mn, Zn, Cu, Ni, Cr, Cd, Pb, and As, were determined in treated shoots, high-performance liquid chromatography (HPLC) was used to identify and quantify phenolic compounds, total phenolic, and flavonoid contents, while free radical scavenging activities were determined using 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) assays.</p><p><strong>Results: </strong>Results showed that adding silicate at 1.0 mg L^- 1 enhanced shoot number, leaf number, chlorophyll, growth vigour, leaf area, fresh and dry weight, and significantly reduced vitrification. Nineteen compounds were identified, with fig shoot extracts showing significant variation in the contents of phenolic acids and flavonoids.</p><p><strong>Conclusion: </strong>The use of potassium silicate enhanced fig growth, elemental content, and pharmacological components; however, a high rate (10.0 mg L^- 1) diminished most parameters, with the exception of Na and Cr. Further studies using low rates of traditional and nanoparticles of silicate are recommended.</p>","PeriodicalId":9234,"journal":{"name":"BMC Research Notes","volume":" ","pages":""},"PeriodicalIF":1.7,"publicationDate":"2026-03-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13064410/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147364208","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Association between Epstein-Barr virus capsid antigen IgA positivity and increased risk of chronic obstructive pulmonary disease: a retrospective cohort study.","authors":"Nien-Chieh Liao, Ming-Che Lee, Heng-Wei Chai, Jia-Jyuan Li, Fang-Ju Shen, Shang-Feng Yang","doi":"10.1186/s13104-026-07698-6","DOIUrl":"10.1186/s13104-026-07698-6","url":null,"abstract":"","PeriodicalId":9234,"journal":{"name":"BMC Research Notes","volume":" ","pages":""},"PeriodicalIF":1.7,"publicationDate":"2026-03-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13072517/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147364210","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}