BMC Research Notes最新文献_第9页

Investigating microbial population structure and function in the chicken caeca and large intestine over time using metagenomics. 利用宏基因组学研究鸡盲肠和大肠随时间变化的微生物种群结构和功能。

IF 1.7

BMC Research Notes Pub Date : 2025-08-15 DOI: 10.1186/s13104-025-07441-7

Banaz Star-Shirko, Gladys Maria Pangga, Aaron McKenna, Nicolae Corcionivoschi, Anne Richmond, Umer Zeeshan Ijaz, Ozan Gundogdu

{"title":"Investigating microbial population structure and function in the chicken caeca and large intestine over time using metagenomics.","authors":"Banaz Star-Shirko, Gladys Maria Pangga, Aaron McKenna, Nicolae Corcionivoschi, Anne Richmond, Umer Zeeshan Ijaz, Ozan Gundogdu","doi":"10.1186/s13104-025-07441-7","DOIUrl":"10.1186/s13104-025-07441-7","url":null,"abstract":"Objectives: Although taxonomic variations in chicken gut microbiota have been previously documented, their functional capacity remain poorly understood. To gain a better understanding, we incorporated whole genome shotgun metagenomics to analyse microbial communities of two different organs: the caeca and the large intestine.Results: Using 24 samples obtained from the caeca and the large intestine of commercial chickens, we assembled Metagenome-Assembled Genomes (MAGs) and characterise their functional profiles. Afterwards, using 8 samples, we integrated this sequencing data with chicken performance metadata body weight (BW), weight gain, feed intake (FI), feed conversion ratio (FCR) and age. MAGs belonging to specific families were found to be positively associated with changes in performance parameters. Functional analyses suggest changes in nutrient geochemical cycles including hydrogen generation within the carbon-cycle. Furthermore, 108 CAZymes were identified for MAGs belonging to two major families - glycoside hydrolase (GH) and polysaccharide lyase (PL), which are important for breakdown of dietary carbohydrates and fibres. A total of 13 polysaccharide lyases were identified functioning on day 20 with enzymes were specific to organs. Overall, our results provide a deeper understanding of microbial-mediated metabolism concerning key performance parameters in chicken production.","PeriodicalId":9234,"journal":{"name":"BMC Research Notes","volume":"18 1","pages":"355"},"PeriodicalIF":1.7,"publicationDate":"2025-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12357378/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144858929","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Investigation of antibiofilm activity of bacteriophage ETEC-phage-TG and BC-VP against pathogenic Escherichia coli biofilms. 噬菌体tec -噬菌体tg和BC-VP对致病性大肠杆菌生物膜的抗菌活性研究。

IF 1.7

BMC Research Notes Pub Date : 2025-08-14 DOI: 10.1186/s13104-025-07432-8

King Archell, Javier Stuart, Diana Elizabeth Waturangi

{"title":"Investigation of antibiofilm activity of bacteriophage ETEC-phage-TG and BC-VP against pathogenic Escherichia coli biofilms.","authors":"King Archell, Javier Stuart, Diana Elizabeth Waturangi","doi":"10.1186/s13104-025-07432-8","DOIUrl":"10.1186/s13104-025-07432-8","url":null,"abstract":"Objectives: The aim of this study was to determine the antibiofilm activity of bacteriophages, ETEC-phage-TG and BC-VP, isolated from freshwater lakes against biofilm of Enterohemorrhagic Escherichia coli and Enteropathogenic Escherichia coli on polystyrene and stainless-steel surfaces.Results: Bacteriophages isolated from previous research have the potential to degrade biofilm. Bacteriophage ETEC-Phage-TG have titer 4.50 × 105 PFU/mL towards EHEC and a Minimum Inhibitory Multiplicity of Infection of 0.01 (10- 2). Bacteriophage BC-VP had titer 8.0877 × 104 PFU/mL towards EPEC and a Minimum Inhibitory Multiplicity of Infection of 0.001 (10- 3). The bacteriophage ETEC-phage-TG was able to inhibit and destruct biofilm formation on polystyrene surface at 25.43% and 11.41% respectively. Bacteriophage ETEC-phage-TG was able to destruct biofilm formation (24 h and 48 h) on stainless steel surface at 37.87% and 39.21% respectively. Bacteriophage BC-VP was able to inhibit and destruct biofilm on polystyrene surface at 54.57% and 26.36% respectively, it was also able to destruct biofilm formation (24 h and 48 h) on stainless steel surface 33.21% and 43.94% respectively.","PeriodicalId":9234,"journal":{"name":"BMC Research Notes","volume":"18 1","pages":"354"},"PeriodicalIF":1.7,"publicationDate":"2025-08-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12355851/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144854690","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dental caries prevention profile of children with and without sickle cell anaemia in a suburban community in Nigeria. 尼日利亚郊区社区镰状细胞性贫血儿童和非镰状细胞性贫血儿童龋齿预防概况

IF 1.7

BMC Research Notes Pub Date : 2025-08-14 DOI: 10.1186/s13104-025-07418-6

Jacob Babatunde Afolabi, Elizabeth Obhioneh Oziegbe, Samuel Ademola Adegoke, Olufemi Adefehinti, Elijah Olufemi Oyetola, Moréniké Oluwátóyìn Foláyan

{"title":"Dental caries prevention profile of children with and without sickle cell anaemia in a suburban community in Nigeria.","authors":"Jacob Babatunde Afolabi, Elizabeth Obhioneh Oziegbe, Samuel Ademola Adegoke, Olufemi Adefehinti, Elijah Olufemi Oyetola, Moréniké Oluwátóyìn Foláyan","doi":"10.1186/s13104-025-07418-6","DOIUrl":"10.1186/s13104-025-07418-6","url":null,"abstract":"Background: The cascade of events in children with sickle cell anaemia (SCA) may influence their caries experience. This case-control study aimed to compare the dental caries prevention profile (captured via caries experience, risk behaviours, and access patterns) of the primary and permanent dentitions of children with and without SCA in a suburban community in Nigeria.Results: This is a sub-study of a larger case-control study that recruited 264 children (120 males, 144 females; mean age 9.41 ± 3.35 years). Among those with primary dentition (n = 207), 13.5% had dental caries. Children with SCA had twice the dental caries experience as those without (17.9% vs. 8.9%), though not statistically significant (p = 0.06). No significant associations were found between caries and tooth brushing, daily fluoride use, or frequency of daily consumption of refined carbohydrates. However, caries was significantly higher among children who had used dental services (50% vs. 10.9%; p < 0.001). The dmft score was significantly higher in children with SCA (p = 0.04) and girls (p = 0.02). In permanent teeth, children with SCA with fair hygiene had higher DMFT scores (p = 0.03).Clinical trial number: Not applicable.","PeriodicalId":9234,"journal":{"name":"BMC Research Notes","volume":"18 1","pages":"353"},"PeriodicalIF":1.7,"publicationDate":"2025-08-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12355780/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144854689","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

3D-printed cuvette holder clip for a spectrophotometer. 用于分光光度计的3d打印比色管支架夹。

IF 1.7

BMC Research Notes Pub Date : 2025-08-13 DOI: 10.1186/s13104-025-07424-8

Warangkana Yimkosol, Nungnit Wattanavichean, Manchuta Dangkulwanich

引用次数: 0

Exploring novel blood-based DNA methylation biomarkers for alzheimer's disease via targeted sequencing of highly variable CpG sites. 通过对高度可变的CpG位点进行靶向测序，探索阿尔茨海默病的新型血液DNA甲基化生物标志物。

IF 1.7

BMC Research Notes Pub Date : 2025-08-12 DOI: 10.1186/s13104-025-07417-7

Hideki Ohmomo, Shohei Komaki, Shiori Minabe, Yoichi Sutoh, Yayoi Otsuka-Yamasaki, Makoto Sasaki, Atsushi Shimizu

引用次数: 0

Microbial diversity in cutaneous leishmaniasis lesions and potential implications for disease progression and treatment outcomes. 皮肤利什曼病病变中的微生物多样性及其对疾病进展和治疗结果的潜在影响

IF 1.7

BMC Research Notes Pub Date : 2025-08-12 DOI: 10.1186/s13104-025-07420-y

Nayana Gunathilaka, Thulangi Siriwardana, Saranga Erathna, Wasana Rodrigo, Hiran Gunasekara, Buthsiri Sumanasena

{"title":"Microbial diversity in cutaneous leishmaniasis lesions and potential implications for disease progression and treatment outcomes.","authors":"Nayana Gunathilaka, Thulangi Siriwardana, Saranga Erathna, Wasana Rodrigo, Hiran Gunasekara, Buthsiri Sumanasena","doi":"10.1186/s13104-025-07420-y","DOIUrl":"10.1186/s13104-025-07420-y","url":null,"abstract":"Objective: Beyond the parasitic infection in Cutaneous leishmaniasis (CL), secondary bacterial colonization can influence disease chronicity, delay healing, and reduce treatment efficacy. This study investigated the bacterial diversity in CL lesions, its association with lesion duration, and its potential impact on treatment outcomes among Sri Lankan patients.Results: Fifteen bacterial species were identified, including both Gram-positive and Gram-negative organisms. Staphylococcus aureus was associated with the longest lesion duration (up to 12 months) and extended treatment (15 cycles of intralesional sodium stibogluconate and cryotherapy). In contrast, species such as Kocuria palustris and Acinetobacter baylyi were linked to shorter treatment durations. Multivariate analysis revealed that lesion type significantly influenced treatment duration (P < 0.05), while larger lesion size and diabetes showed marginal associations with prolonged therapy. The presence of opportunistic and antibiotic-resistant species, particularly S. aureus, suggests a potential contributory role of bacterial co-infections in CL progression and highlights the need to consider their presence in treatment planning. Integrating microbial profiling into clinical protocols may enhance treatment efficacy and inform personalized care strategies. However, the limited sample size and convenience-based recruitment may affect the generalizability of these findings, and the potential influence of bacterial colonization on treatment response warrants further investigation in larger cohorts.","PeriodicalId":9234,"journal":{"name":"BMC Research Notes","volume":"18 1","pages":"351"},"PeriodicalIF":1.7,"publicationDate":"2025-08-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12344993/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144834067","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The statistical relationship between sweet consumption and academic achievement among Palestinian university students: a cross-sectional study. 巴勒斯坦大学生甜食消费与学业成绩的统计关系：一项横断面研究。

IF 1.7

BMC Research Notes Pub Date : 2025-08-11 DOI: 10.1186/s13104-025-07425-7

Inad Nawajah

{"title":"The statistical relationship between sweet consumption and academic achievement among Palestinian university students: a cross-sectional study.","authors":"Inad Nawajah","doi":"10.1186/s13104-025-07425-7","DOIUrl":"10.1186/s13104-025-07425-7","url":null,"abstract":"Objective: This study examined the relationship between sweet consumption and academic performance among medical university students in Palestine. A cross-sectional survey was conducted among full-time students selected via stratified sampling. Data on academic performance (self-reported GPA), sweet consumption frequency, and potential confounders (demographics, BMI, parental education, income, breakfast consumption, physical activity, and mental health via the Strengths and Difficulties Questionnaire) were collected using structured questionnaires. Multiple linear regression models were used to assess associations.Results: Of 355 invited students, 220 completed the survey (response rate: 62%). Nearly half (48.2%) reported consuming sweets daily or more. Frequent sweet consumption was significantly associated with lower GPA in both crude and fully adjusted models (β = - 0.15; 95% CI: - 0.29, - 0.01; p = 0.039). Low physical activity (β = - 0.22; p = 0.008) and abnormal mental health scores (β = - 0.31; p = 0.004) were also independently linked to poorer academic performance. Frequent sweet intake is independently associated with reduced academic achievement among university students, even after adjusting for lifestyle and mental health factors. Promoting healthy eating and mental well-being may support academic success in this population.Clinical trial number: Not applicable.","PeriodicalId":9234,"journal":{"name":"BMC Research Notes","volume":"18 1","pages":"349"},"PeriodicalIF":1.7,"publicationDate":"2025-08-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12341343/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144820655","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A qualitative study on cultural sensitivity during survey fieldwork for early childhood caries in Ile-Ife, Nigeria: bridging the gap between culture and science. 尼日利亚Ile-Ife幼儿龋病实地调查期间文化敏感性的定性研究：弥合文化与科学之间的差距。

IF 1.7

BMC Research Notes Pub Date : 2025-08-11 DOI: 10.1186/s13104-025-07422-w

Moréniké Oluwátóyìn Foláyan, Olaniyi Arowolo, Simin Z Mohebbi, Mohammad R Khami

{"title":"A qualitative study on cultural sensitivity during survey fieldwork for early childhood caries in Ile-Ife, Nigeria: bridging the gap between culture and science.","authors":"Moréniké Oluwátóyìn Foláyan, Olaniyi Arowolo, Simin Z Mohebbi, Mohammad R Khami","doi":"10.1186/s13104-025-07422-w","DOIUrl":"10.1186/s13104-025-07422-w","url":null,"abstract":"Objective: The qualitative study described culture-related issues that may bridge the ethical conduct of fieldwork for a cross-sectional study of the oral health of preschool children in the Ife Central Local Government Area of Nigeria, conducted between December 2024 and January 2025.Results: There were 1,411 mother‒child pairs recruited by 20 research assistants and nine dentists trained on ethical considerations and cultural sensitivity before data collection began. A group discussion (debriefing session) was held with 18 consenting research assistants and nine dentists of the fieldwork. The transcripts from the discussions were inductively analysed, and key themes were identified. Both research assistants and dentists observed cultural norms infringing on the privacy of study participants, and dentists had to overcome mistrust and consent challenges (concerns with touching the child's head during height measurement and with dentists conducting oral examinations). This study highlights the need to map the cultural nuances of study sites before conducting a population-level survey to help develop culturally sensitive study protocols.","PeriodicalId":9234,"journal":{"name":"BMC Research Notes","volume":"18 1","pages":"347"},"PeriodicalIF":1.7,"publicationDate":"2025-08-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12337499/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144820653","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Faculty development for junior educators: advancing the Scholarship of Teaching and Learning (SoTL) Through Co-Teaching and Mentoring. 初级教育工作者的师资发展：通过联合教学和指导推进教与学的学术研究。

IF 1.7

BMC Research Notes Pub Date : 2025-08-11 DOI: 10.1186/s13104-025-07413-x

Fatemeh Keshmiri

{"title":"Faculty development for junior educators: advancing the Scholarship of Teaching and Learning (SoTL) Through Co-Teaching and Mentoring.","authors":"Fatemeh Keshmiri","doi":"10.1186/s13104-025-07413-x","DOIUrl":"10.1186/s13104-025-07413-x","url":null,"abstract":"Objective: The study aimed to evaluate the effect of a faculty development program (FDP) on learner-related outcomes. This longitudinal program was designed by integrating co-teaching and mentoring methods. The FDP specifically focused on enhancing the competencies of junior educators in the Scholarship of Teaching and Learning (SoTL). Within the program, a mentor collaborated with a junior educator to generate ideas, design studies, plan and implement educational strategies, and evaluate outcomes-all aimed at addressing educational challenges or applying innovative methods in the educational setting. Learner-related outcomes were assessed using the three levels (reaction, learning, and behavior) of the Kirkpatrick model.Results: The educators' perception toward SoTL activity and satisfaction were 35.60 ± 4.30 and 4.4 ± 0.7, respectively. The success rate of educators in conducting SoTL projects was 90.4%. The score of SoTL reports in the learning level was 85 ± 8. The productions of SoTL activities, including 38 grant funds, five SoTL festival awards, and nine projects, were published in journals and 10 abstracts. The experiences of the junior educators about the FDP are categorized into a theme of 'team learning in the teaching process'. The combination of co-teaching and mentoring elements created a supportive and collaborative environment in the FDP.","PeriodicalId":9234,"journal":{"name":"BMC Research Notes","volume":"18 1","pages":"348"},"PeriodicalIF":1.7,"publicationDate":"2025-08-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12341095/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144820654","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparison of the inhibitory effects of Lactobacillus supernatant and coculture on Gardnerella vaginalis. 乳酸菌上清液与共培养物对阴道加德纳菌抑制效果的比较。

IF 1.7

BMC Research Notes Pub Date : 2025-08-09 DOI: 10.1186/s13104-025-07414-w

Ferenc Somogyvári, Nándor Tűzkő, Attila Kereszturi, László Párducz, Mária Szécsényi, Valéria Endrész, Marianna Ábrók, Caleb M Ardizzone, Katalin Burián, Dezső Péter Virok

{"title":"Comparison of the inhibitory effects of Lactobacillus supernatant and coculture on Gardnerella vaginalis.","authors":"Ferenc Somogyvári, Nándor Tűzkő, Attila Kereszturi, László Párducz, Mária Szécsényi, Valéria Endrész, Marianna Ábrók, Caleb M Ardizzone, Katalin Burián, Dezső Péter Virok","doi":"10.1186/s13104-025-07414-w","DOIUrl":"10.1186/s13104-025-07414-w","url":null,"abstract":"Objective: Vaginal lactobacilli play a crucial role in inhibiting bacteria such as Gardnerella vaginalis (G. vaginalis), a key contributor to dysbiosis and bacterial vaginosis. We aimed to compare the inhibitory effects of Lactobacillus cell-free supernatants on G. vaginalis growth with those observed in Lactobacillus-G. vaginalis coculture, an experimental setup that more closely mimics in vivo conditions.Results: To identify the optimal medium for coculture experiments, MRS broth and NYC-III broth were compared. NYC-III significantly enhanced the growth of G. vaginalis and four of the five tested Lactobacillus strains. We then developed a direct quantitative PCR (qPCR) method that allowed us to specifically measure G. vaginalis genome concentrations in cocultures with Lactobacillus. This direct qPCR did not require DNA extraction and had a 4,096-fold dynamic range. We then assessed the inhibition of G. vaginalis growth by Lactobacillus cell-free supernatants in G. vaginalis cultures by measuring optical density and the Lactobacillus-mediated inhibition in cocultures by the G. vaginalis specific direct qPCR. The two measurement methods showed different levels of inhibitory activity for two of the five Lactobacillus strains tested. These findings suggest that coculture experiments should be conducted in place of, or in addition to, supernatant-based inhibitory assays.","PeriodicalId":9234,"journal":{"name":"BMC Research Notes","volume":"18 1","pages":"346"},"PeriodicalIF":1.7,"publicationDate":"2025-08-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12335144/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144811820","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0