BMC Research Notes最新文献

{"title":"Evaluating the recovery of pan-susceptible and antibiotic-resistant Escherichia coli in synthetic test agricultural water using membrane filtration and colilert methods.","authors":"Zirui Ray Xiong, Ajani A Brooks, Ellen Gabriel, Alan Gutierrez, Shayla B Johnson, Cheryl East, Lisa M Durso, Elica Moss, Manan Sharma","doi":"10.1186/s13104-025-07423-9","DOIUrl":"https://doi.org/10.1186/s13104-025-07423-9","url":null,"abstract":"<p><p>Membrane filtration and Colilert assays are commonly used to quantify Escherichia coli levels in agricultural water. These methods have not been evaluated in test agricultural water (TAW), a formulation used for assessing chemical sanitizer effectiveness. Quantitative recovery of E. coli in TAW may be affected by turbidity levels. TAW was formulated with a pH value of 6.5 at two turbidity levels (0 and 50 NTU). Pan-susceptible and cefotaxime-resistant E. coli strains were added separately to TAW (200 CFU/100 mL). Inoculated TAW was either (1) filtered using a 0.45 μm membrane filter (MF) and placed onto Tryptone Bile X-Glucuronide (TBX) agar or CHROMagar ECC (ECC), or (2) tested by IDEXX Quanti-Tray/2000 Colilert. Recovery percentages of E. coli at both turbidity levels were similar on TBX and ECC, but pan-susceptible E. coli ARS C101 in TAW at 50 NTU on TBX was not quantifiable. For Colilert assays, the recovery percentages of E. coli from 0 NTU and 50 NTU TAW were between 93.4 ± 4.4% and 127.0 ± 10.5%. Overall, ECC and Colilert provided quantifiable results for recovery of pan-susceptible and cefotaxime-resistant E. coli from artificial agricultural water at different turbidity levels. Higher levels of turbidity affected the ability to quantify E. coli by MF with TBX and recovery by Colilert.</p>","PeriodicalId":9234,"journal":{"name":"BMC Research Notes","volume":"18 1","pages":"365"},"PeriodicalIF":1.7,"publicationDate":"2025-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12366208/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144943458","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Menstrual hygiene management practices and associated factors among primary school girls in Gakenke district, Rwanda.","authors":"Thamar Niwemukiza, Monica Mochama, Amanuel Kidane Andegiorgish","doi":"10.1186/s13104-025-07429-3","DOIUrl":"https://doi.org/10.1186/s13104-025-07429-3","url":null,"abstract":"<p><strong>Background: </strong>Menstruating girls encounter persistent social, cultural, and religious restrictions, alongside inadequate facilities at home and school, hindering effective menstrual hygiene management (MHM). In rural Rwanda, limited awareness and preparedness worsen these challenges. This study investigates MHM practices and associated factors among primary schoolgirls in the Gakenke District to inform targeted interventions.</p><p><strong>Materials and methods: </strong>An institution-based cross-sectional study of 384 primary school girls was conducted. A systematic sampling technique was used for recruiting participants. The data were collected using a structured questionnaire and were entered in Excel, which was subsequently sent to SPSS V. 25 for analysis. Bivariate and multivariate logistic regression analyses were used to test associations. Two-tailed P-value < 0.05 was considered statistically significant.</p><p><strong>Results: </strong>The mean age of the study participants was 14 years (1.3 years). About one in ten (9.11%) school-aged girls in Gakenke District, Rwanda, had poor knowledge of MHM, 11.2% had negative attitudes toward MHM, and 14.3% had poor practices. The odds of good MHM practices were significantly greater among school girls who knew that their menstrual blood came from the uterus (AOR: 4.57; 95% CI: 1.62 to 12.89), who knew that menstruation is a monthly cycle (AOR: 2.86; 95% CI: 2.08 to 15.75), who received formal puberty or menstrual education (AOR: 2.18; 95% CI: 1.19 to 9.13), who knew that disposable and reusable pads are available on the market (AOR: 7.06; 95% CI: 5.37 to 18.58), who had good knowledge of MHM (AOR: 3.32; 95% CI: 1.87 to 21.05) and who had a positive attitude toward MHH (AOR: 11.44; 95% CI: 9.60 to 16.02).</p><p><strong>Conclusion: </strong>Addressing poor MHM among young adolescents requires collaborative interventions, including stakeholder-led sensitization for formal puberty education, improved access to disposable and reusable pads, and initiatives that boost knowledge and foster positive attitudes toward menstruation.</p>","PeriodicalId":9234,"journal":{"name":"BMC Research Notes","volume":"18 1","pages":"366"},"PeriodicalIF":1.7,"publicationDate":"2025-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12369164/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144943623","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Whole genome resequencing data of the Iranian cashmere goat breed.","authors":"Elham Rezvannejad, Hojjat Asadollahpour Nanaei, Zeinab Amiri Ghanatsaman","doi":"10.1186/s13104-025-07431-9","DOIUrl":"10.1186/s13104-025-07431-9","url":null,"abstract":"<p><strong>Objective: </strong>The Raeini Cashmere goat, native to Kerman Province, Iran, is renowned for its high-quality cashmere fiber, a luxury natural fiber valued for its softness, warmth, and lightweight properties in the textile industry. This breed, adapted to the region's arid climate through long-term natural and artificial selection, supports the local agricultural economy through cashmere, meat, and milk production. This study presents the first whole-genome resequencing dataset for the Raeini Cashmere goat, providing a valuable resource for ecological, evolutionary, and population genomic studies.</p><p><strong>Data description: </strong>Genomic DNA was extracted from blood samples of ten individual Raeini Cashmere goats and sequenced using the Illumina NovaSeq 2500 platform. The dataset, deposited in the NCBI Sequence Read Archive (accession PRJNA1090775), enables future research into genetic adaptations for cashmere production and environmental resilience, supporting breeding programs and comparative genomics.</p>","PeriodicalId":9234,"journal":{"name":"BMC Research Notes","volume":"18 1","pages":"363"},"PeriodicalIF":1.7,"publicationDate":"2025-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12366087/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144882143","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Knowledge and attitudes towards dementia among health professionals in Somaliland: a pilot survey.","authors":"Mohamed Abdilahi Duale, Tewelde Tesfaye Gebremariam","doi":"10.1186/s13104-025-07446-2","DOIUrl":"10.1186/s13104-025-07446-2","url":null,"abstract":"<p><strong>Objectives: </strong>This study was aimed to evaluate the knowledge and attitudes of healthcare professionals in Somaliland towards dementia.</p><p><strong>Methods: </strong>Between June and July 2023, we administered an adapted online survey consisting of 30 Alzheimer's disease Knowledge Scale (ADKS) true or false statements and 10 Dementia Care Attitude Scale (DCAS) Likert-scale items. The reliability of the Likert-scale DCAS items was assessed using Cronbach's alpha. The distribution of responses to DCAS statements was examined using a binomial test, and the relationship between knowledge or attitude and sample characteristics was assessed using Pearson's chi-squared test or Fisher's exact test.</p><p><strong>Results: </strong>The overall validity (Cronbach's alpha) for DCAS statements (0.88) was greater than 0.80, indicating good level of internal consistency. Out of 107 healthcare workers who completed the survey, 53.3% had unfavourable attitude towards dementia care, and 49.5% had a moderate-to-high knowledge level.</p><p><strong>Conclusions: </strong>Our pilot survey revealed a lack of knowledge and attitudes about dementia among health professionals and urges high quality surveys to validate the results.</p>","PeriodicalId":9234,"journal":{"name":"BMC Research Notes","volume":"18 1","pages":"360"},"PeriodicalIF":1.7,"publicationDate":"2025-08-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12363011/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144871584","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Level of job satisfaction and its associated factors among midwives working in public health institutions, Northwest Ethiopia: a multi-center study.","authors":"Nebiyu Solomon Tibebu, Berihun Agegn Mengistie, Saron Abeje Abiye, Paulos Abeje, Selamawit Tilahun, Sosina Belay, Geremew Azene, Belayneh Ayanaw Kassie","doi":"10.1186/s13104-025-07442-6","DOIUrl":"10.1186/s13104-025-07442-6","url":null,"abstract":"<p><strong>Introduction: </strong>Midwives are practitioners of midwifery and are recognized as indispensable professionals for lowering maternal mortality worldwide, particularly in low-income countries. Midwives who aren't satisfied with their job challenge the prevention and control of maternal mortality and morbidity and had a surge in unfavorable patient outcomes. Therefore, this study aimed to assess the level of job satisfaction and its associated factors among midwives working at public health institutions in northwest Ethiopia.</p><p><strong>Methods: </strong>An institutional-based cross-sectional study was conducted from February 1st to July 15th, 2022. A total of 422 midwives participated in the study. A simple random sampling method was used to select all eligible participants. Data were collected using a semi-structured, pretested, and self-administered questionnaire. Data version 4.6 software and SPSS 25 were used for data entry and analysis, respectively. Both bivariable and multivariable logistic regression analyses were conducted to find factors associated with midwives' job satisfaction. The statistical association was decided based on the adjusted odds ratio (AOR) with its 95% confidence interval (CI) and p-value of < 0.05.</p><p><strong>Results: </strong>The prevalence of midwives' satisfaction with their job was 46.7% (95% CI: 41.8, 52.0). Institutions followed democratic leadership (AOR = 2.03, 95% CI: 1.02, 4.40), having good relationships with their superiors (AOR = 2.01, 95% CI: 1.00, 5.02), being satisfied with payment and benefits (AOR = 4.00, 95% CI: 1.45, 9.12), and safe job security (AOR = 5.66, 95% CI: 4.8, 12.68) were found to be statistically significant factors associated with midwives' job satisfaction.</p><p><strong>Conclusion and recommendations: </strong>In this study the level of midwives' job satisfaction was low. Thus, setting strategies in exercising democratic leadership, promoting good relations with superiors, providing incentives opportunities, and ensuring work security will have significant role in improving midwives' satisfaction with their job.</p>","PeriodicalId":9234,"journal":{"name":"BMC Research Notes","volume":"18 1","pages":"362"},"PeriodicalIF":1.7,"publicationDate":"2025-08-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12363070/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144871585","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessing patients' knowledge immediately after obtaining the antibacterial drug azithromycin: a community pharmacy-based cross-sectional study.","authors":"Asmaa S Mohamed, Hosam M A Refaei, Remon R Rofaeil","doi":"10.1186/s13104-025-07447-1","DOIUrl":"10.1186/s13104-025-07447-1","url":null,"abstract":"<p><strong>Objective: </strong>This cross-sectional study was conducted across 38 community pharmacies and included 418 patients who were dispensed oral azithromycin. Pharmacists administered a structured questionnaire to collect demographic information and assess patients' knowledge regarding azithromycin use. Knowledge was evaluated based on responses related to the medication's name, dosage, frequency, potential side effects, and intended purpose. Based on their scores, participants were categorized as having poor (< 50%), average (50-70%), or good (> 70%) knowledge. The aim of this study was to assess patients' knowledge of azithromycin use and identify factors influencing their level of understanding.</p><p><strong>Results: </strong>A total of 418 participants completed the questionnaire, with a mean age of 35.27 years; 75.60% were under 40 years old, and 62.20% were female. The majority were married (81.82%), resided in urban areas (57.89%), and held a university degree (67.70%). Azithromycin was prescribed by physicians for 62.20% of participants, while 29.67% obtained it directly from pharmacists via over-the-counter (OTC) access, and 8.13% self-medicated. Knowledge scores differed significantly according to marital status, place of residence, education level, and socioeconomic status, with higher scores observed among married, urban, university-educated, and higher-income individuals (p < 0.05). In contrast, age, gender, smoking status, and diabetes mellitus showed no significant association with knowledge scores. Overall, 21.53% of participants had poor knowledge, 46.41% had average knowledge, and 32.06% demonstrated good knowledge.</p>","PeriodicalId":9234,"journal":{"name":"BMC Research Notes","volume":"18 1","pages":"358"},"PeriodicalIF":1.7,"publicationDate":"2025-08-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12359916/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144871582","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Telomere length measures in the Northern Ireland cohort for the longitudinal study of ageing (NICOLA).","authors":"Claire Hill, Laura Smyth, Jill Kilner, Katie Quinn, Angie Scott, Charlotte Neville, Frank Kee, Bernadette McGuinness, Amy Jayne McKnight","doi":"10.1186/s13104-025-07393-y","DOIUrl":"10.1186/s13104-025-07393-y","url":null,"abstract":"<p><strong>Objectives: </strong>Using blood derived DNA collected from individuals within the Northern Ireland COhort for the Longitudinal study of Ageing (NICOLA), quantitative real-time polymerase chain reaction (RT-PCR) based absolute telomere length measures were derived in triplicate. This data is generated on a subset of participants (n = 2,971) within the NICOLA cohort at Wave 1 baseline. NICOLA commenced Wave 3 of data collection in Autumn 2024.</p><p><strong>Data description: </strong>The NICOLA study recruited approximately 8,500 people from across Northern Ireland, with individuals recruited as representative of the Northern Ireland population. At recruitment, the NICOLA cohort was 45% male and 55% female. Adults were recruited over the age of 50, with approximately 50% of participants aged between 50 and 65, and 10% of participants over the age of 80. Telomere length (TL) values were determined for 2,971individuals (47.7% male) across 33 batches using the Absolute Human Telomere Length Quantification qPCR Assay kit and Roche LightCycler 480 II. Within-batch duplicate measures were established, as were water controls and non-template controls.</p>","PeriodicalId":9234,"journal":{"name":"BMC Research Notes","volume":"18 1","pages":"359"},"PeriodicalIF":1.7,"publicationDate":"2025-08-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12359945/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144871586","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Factors determining diversity of saproxylic arthropods in the fruiting bodies of the birch polypore fungus (Fomitopsis betulina).","authors":"Craig D Perl","doi":"10.1186/s13104-025-07434-6","DOIUrl":"10.1186/s13104-025-07434-6","url":null,"abstract":"<p><strong>Objective: </strong>Certain basidiomycete fungi produce semi-stable fruiting bodies, known as brackets, that support a significant quality of saproxylic invertebrate biodiversity, especially in northern forests. The objective of this investigation was to assess the drivers of saproxylic diversity within the fruiting bodies of Fomitopsis betulina. We explore which factors are significant determinants of arthropod diversity and abundance, examining the effects of sporocarp size, height above the ground, and relative isolation from neighbouring sporocarps.</p><p><strong>Results: </strong>We find that larger sporocarps support a greater number of arthropods, but diversity and species richness are determined by distance from nearest neighbour.</p>","PeriodicalId":9234,"journal":{"name":"BMC Research Notes","volume":"18 1","pages":"361"},"PeriodicalIF":1.7,"publicationDate":"2025-08-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12363027/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144871583","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The association between GERD symptoms severity and psychosocial health in palestine: a cross-sectional study.","authors":"Qusay Abdoh, Fatima Masoud Al-Amouri, Mohammad Taleb Abed, Mohammed Motasem Jaber, Nour Mahmoud Yassin, Raheeq Mohannad Shawahne, Hiba Sadder, Manal Badrasawi","doi":"10.1186/s13104-025-07449-z","DOIUrl":"10.1186/s13104-025-07449-z","url":null,"abstract":"<p><strong>Objective: </strong>This study aimed to investigate the relationship between GERD and mental health status, alongside lifestyle factors and eating patterns among Palestinian adults.</p><p><strong>Results: </strong>In this study, 144 participants with GERD were analyzed. GERD symptom severity was categorized into three groups: mild (35.4%), moderate (32.0%), and severe (32.6%), according to the GERD-HRQOL. Multiple linear regression analysis revealed that sleeping problems are the only predictor of GERD symptoms severity (B = 3.785, 95% confidence interval = 0.712-6.857, p = 0.016).</p>","PeriodicalId":9234,"journal":{"name":"BMC Research Notes","volume":"18 1","pages":"357"},"PeriodicalIF":1.7,"publicationDate":"2025-08-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12359991/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144871587","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"MicroRNA profiles of four induced pluripotent stem cell lines derived from distinct tissues.","authors":"Kongtana Trakarnsanga, Nutchavadee Vorasan, Nutnicha Tantarungsee, Methichit Wattanapanitch, Chinnavuth Vatanashevanopakorn, Bhoom Suktitipat, Chanatip Metheetrairut","doi":"10.1186/s13104-025-07437-3","DOIUrl":"10.1186/s13104-025-07437-3","url":null,"abstract":"<p><strong>Objective: </strong>Induced pluripotent stem cells (iPSCs) are generated from a vast number of adult cell types. While they all acquired embryonic stem cell (ESC)-like properties during reprogramming, differences in certain characteristics, including differentiation potential, remained. These differences are hypothesized to be due to epigenetic memory or individual genetic background.</p><p><strong>Results: </strong>This study compared microRNA (miRNA) profiles, which is one aspect of epigenetic regulation, of four iPSC lines derived from dermal fibroblasts, hair follicle keratinocytes, peripheral blood T cells, and exfoliated renal epithelial cells by nCounter^® miRNA expression panels. 110 miRNAs were expressed in all iPSC lines, which accounted for 67.5-75.9% of expressed miRNAs in each line, while there were only 3, 4, 9, and 28 uniquely expressed miRNAs in each line. MiRNA profiles of the four iPSC lines clustered together, but away from those of mature cells of origin or ESCs retrieved from the Gene Expression Omnibus database as analyzed by principal component analysis. These results suggested that the miRNA profiles of the four iPSC lines are mostly similar to each other, though with some specific unique gene expression, and do not appear to indicate any obvious differences between the iPSC lines derived from different adult cell types in terms of miRNA expression.</p>","PeriodicalId":9234,"journal":{"name":"BMC Research Notes","volume":"18 1","pages":"356"},"PeriodicalIF":1.7,"publicationDate":"2025-08-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12358071/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144862085","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}