BMC Research Notes最新文献_第7页

CD4 cell count and viral load count association and its joint risk factors among adult TB/HIV co-infected patients: a retrospective follow-up study. 成人TB/HIV合并感染患者CD4细胞计数和病毒载量计数的相关性及其联合危险因素：一项回顾性随访研究

IF 1.7

BMC Research Notes Pub Date : 2025-09-01 DOI: 10.1186/s13104-025-07428-4

Nurye Seid Muhie, Awoke Seyoum Tegegne

{"title":"CD4 cell count and viral load count association and its joint risk factors among adult TB/HIV co-infected patients: a retrospective follow-up study.","authors":"Nurye Seid Muhie, Awoke Seyoum Tegegne","doi":"10.1186/s13104-025-07428-4","DOIUrl":"10.1186/s13104-025-07428-4","url":null,"abstract":"Objective: The aim of this study was to determine CD4 cell count and viral load count association and its joint clinical risk factors among adult TB/HIV co-infected patients. The purpose of this research was leads to contribute improved planning and execution of screening programs, regular follow-ups, and prevention measures.Result: The baseline variability for viral load ([Formula: see text]) was higher than CD4 cell (819.797). Furthermore, the correlation matrix (R) shows that the baseline subject-specific CD4 cell and viral load were negatively correlated (- 0.[Formula: see text]). There was also a negative relationship between the subject-specific change CD4 cell and baseline viral load (-[Formula: see text]), subject-specific baseline CD4 cell, and the patient-specific change of viral load (-0.6513), longitudinal trajectory (association of evolution) for the two random slopes [Formula: see text]-0.9413). Then, there was very strong negative relationship between subject-specific change CD4 cell and viral load over time. Visit time[Formula: see text], white blood cell[Formula: see text], good[Formula: see text] and fair[Formula: see text]) adherence, hemoglobin ≥ 11 g/dl[Formula: see text], baseline CD4 cell ≥ 200 cells/mm3[Formula: see text], and baseline viral load < 10,000 copies/mL[Formula: see text]hematocrit[Formula: see text]and monocytes of patients[Formula: see text] were a joint determinates for viral load and CD4 cell, respectively.","PeriodicalId":9234,"journal":{"name":"BMC Research Notes","volume":"18 1","pages":"377"},"PeriodicalIF":1.7,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12403304/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144943207","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Smart thin porous calcium phosphate coatings for local antibiotic delivery. 智能薄多孔磷酸钙涂层局部抗生素输送。

IF 1.7

BMC Research Notes Pub Date : 2025-09-01 DOI: 10.1186/s13104-025-07453-3

Long-Quan R V Le, Maria Carolina Lanzino, Anika Höppel, Mirjam Rech, Sofia Dembski, Andreas Killinger, Bianca Riedel, Michael Seidenstuecker

{"title":"Smart thin porous calcium phosphate coatings for local antibiotic delivery.","authors":"Long-Quan R V Le, Maria Carolina Lanzino, Anika Höppel, Mirjam Rech, Sofia Dembski, Andreas Killinger, Bianca Riedel, Michael Seidenstuecker","doi":"10.1186/s13104-025-07453-3","DOIUrl":"10.1186/s13104-025-07453-3","url":null,"abstract":"Objective: Implant failure after arthroplasty, primarily due to aseptic loosening or periprosthetic joint infection, remains a significant clinical problem. Bioactive ceramic coatings, such as β-tricalcium phosphate (β-TCP), enhance osseointegration and may reduce the risk of aseptic loosening. At the same time, localized antibiotic release from the implant surface represents a promising strategy to prevent early bacterial colonization. The aim of this study was to evaluate the feasibility of incorporating the heat-sensitive antibiotic vancomycin (VAN) into β-TCP coatings using high-velocity suspension flame spraying (HVSFS).Results: We successfully embedded VAN into β-TCP coatings by preparing suspensions containing VAN-loaded supraparticles as feedstock for the HVSFS process. High-performance liquid chromatography analysis confirmed that VAN maintained its chemical integrity during spraying, with spectra comparable to untreated controls, indicating no thermal degradation. The resulting multifunctional coatings therefore combined the osteoconductive potential of β-TCP with the antibacterial activity of VAN. These findings demonstrate that HVSFS is a viable technique for producing bioactive coatings that simultaneously promote bone integration and enable local antibiotic delivery, offering a potential strategy to mitigate both aseptic loosening and infection risks in arthroplasty.","PeriodicalId":9234,"journal":{"name":"BMC Research Notes","volume":"18 1","pages":"375"},"PeriodicalIF":1.7,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12400540/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144943610","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Task sharing and teamwork: clinician preferences for alcohol screening and brief interventions in cardiology. 任务分担和团队合作：临床医生对心脏病学中酒精筛查和简短干预的偏好。

IF 1.7

BMC Research Notes Pub Date : 2025-08-29 DOI: 10.1186/s13104-025-07452-4

Paul Welfordsson, Anna-Karin Danielsson, Caroline Björck, Bartosz Grzymala-Lubanski, Kristina Hambraeus, Ida Haugen Löfman, Frieder Braunschweig, Matthias Lidin, Sara Wallhed Finn

{"title":"Task sharing and teamwork: clinician preferences for alcohol screening and brief interventions in cardiology.","authors":"Paul Welfordsson, Anna-Karin Danielsson, Caroline Björck, Bartosz Grzymala-Lubanski, Kristina Hambraeus, Ida Haugen Löfman, Frieder Braunschweig, Matthias Lidin, Sara Wallhed Finn","doi":"10.1186/s13104-025-07452-4","DOIUrl":"https://doi.org/10.1186/s13104-025-07452-4","url":null,"abstract":"Objective: To investigate clinicians' preferences for alcohol screening and brief interventions in clinical cardiology settings.Results: A total of 664 cardiology clinicians responded to a cross-sectional survey (30.9% response rate), including 55.1% nurses, 21.4% assistant nurses, 18.8% doctors, and 4.7% other clinical staff. Among these participants, 87.5% indicated that patients should be screened for alcohol use on cardiology wards, 79.8% in outpatient clinics, 49.1% in emergency departments, and 45.9% on coronary care units. Doctors and nurses were the preferred professions to be responsible for screening across all clinical contexts, while fewer respondents indicated that assistant nurses or physiotherapists should be responsible for screening (p < .001). Most participants (85.2%) indicated that patients should be screened in more than one clinical context and 84.6% indicated that more than one profession should be responsible for alcohol screening. Clinicians' preferred modality for assessing alcohol use was verbal screening (92% of participants), followed by questionnaires (53.5%), digital tools (28.5%), and alcohol biomarkers (22.1%, p < .001). Just over half of participants (58%) indicated that they would like to attend training on brief interventions. Findings suggest that task sharing, teamwork, and training may be effective strategies for implementation of alcohol screening and brief interventions in clinical cardiology.","PeriodicalId":9234,"journal":{"name":"BMC Research Notes","volume":"18 1","pages":"373"},"PeriodicalIF":1.7,"publicationDate":"2025-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12398004/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144943588","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Poverty and poor choices: socioeconomic gradients of unhealthy behaviors among adults in a multi-center cross-sectional study in Iran. 贫困和不良选择：伊朗一项多中心横断面研究中成年人不健康行为的社会经济梯度

IF 1.7

BMC Research Notes Pub Date : 2025-08-29 DOI: 10.1186/s13104-025-07426-6

Satar Rezaei, Pegah Arefhosseini, Nasim Badiee, Hedayat Salari, Mohammad Bazyar, Mohammad Ranjbar, Eshagh Barfar, Seyed Fahim Irandoost, Javad Moghri, Edris Kakemam

引用次数: 0

In-vivo evaluation of vitamin E loaded muscle void fillers for the provisional treatment of volumetric muscle loss. 体内评估维生素E负载肌肉空隙填充物对体积性肌肉损失的临时治疗。

IF 1.7

BMC Research Notes Pub Date : 2025-08-29 DOI: 10.1186/s13104-025-07454-2

Michael S Valerio, Andrew R Clark, Marissa N Behun, Christopher L Dearth, Stephen M Goldman

{"title":"In-vivo evaluation of vitamin E loaded muscle void fillers for the provisional treatment of volumetric muscle loss.","authors":"Michael S Valerio, Andrew R Clark, Marissa N Behun, Christopher L Dearth, Stephen M Goldman","doi":"10.1186/s13104-025-07454-2","DOIUrl":"https://doi.org/10.1186/s13104-025-07454-2","url":null,"abstract":"Objective: This study investigates the therapeutic potential of α-tocopherol (Vitamin E, VitE), delivered locally via a polyethylene glycol (PEG) hydrogel serving as a muscle void filler (MVF), for the acute treatment of volumetric muscle loss (VML) in a rat model. The primary goal was to determine if VitE, a reactive oxygen species scavenger, could exert antioxidant effects at the VML site and thereby improve the recovery of adjacent muscle tissue over a four-week period.Results: The MVF successfully increased VitE levels in the muscle throughout the implantation period. However, only the lowest concentration of VitE (5 mg/ml) showed a demonstrable effect, resulting in a 30% reduction in peak force compared to the PEG-alone group and a median muscle fiber size that was 4.34 μm smaller. Biochemical analysis revealed no differences in the expression of TGFβ1, protein carbonyl, or malondialdehyde across any groups. While local delivery of VitE was successful, further research is needed to optimize dosing and investigate additional mechanisms for improved muscle recovery.","PeriodicalId":9234,"journal":{"name":"BMC Research Notes","volume":"18 1","pages":"372"},"PeriodicalIF":1.7,"publicationDate":"2025-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12398021/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144943613","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Investigating the effect of Ranolazine drug on the pathophysiology of asthma in a rat model. 探讨雷诺嗪对哮喘大鼠模型病理生理的影响。

IF 1.7

BMC Research Notes Pub Date : 2025-08-29 DOI: 10.1186/s13104-025-07455-1

Maryam Sadeghi, Sarina Shahrooz, Mehdi Aghamohammadi, Hadi Khodabandeloo, Nasrin Amiri-Dashatan, Mehdi Koushki, Seyyed Shamsadin Athari

{"title":"Investigating the effect of Ranolazine drug on the pathophysiology of asthma in a rat model.","authors":"Maryam Sadeghi, Sarina Shahrooz, Mehdi Aghamohammadi, Hadi Khodabandeloo, Nasrin Amiri-Dashatan, Mehdi Koushki, Seyyed Shamsadin Athari","doi":"10.1186/s13104-025-07455-1","DOIUrl":"https://doi.org/10.1186/s13104-025-07455-1","url":null,"abstract":"Objective: Asthma is a common multifactorial inflammatory disease affecting both children and adults. Various cells and cytokines contribute to this inflammatory process. Smooth muscle contraction, vascular congestion, and airway edema lead to airway narrowing, which is an important factor in asthma. This study explores the potential impact of Ranolazine, a sodium blocker used for chronic angina, on asthmatic rats. By investigating the drug's effects on vascular and lung tissue changes, this research seeks to offer perspectives on innovative treatment approaches for asthma.Results: Ranolazine treatment showed minimal impact on serum IgE levels in asthmatic rats, with a slight reduction that was statistically insignificant. However, Ranolazine treatment resulted in a significant decrease in perivascular and peribronchial inflammation levels compared to the asthmatic group, suggesting a potential therapeutic effect on lung histology changes associated with asthma pathophysiology.","PeriodicalId":9234,"journal":{"name":"BMC Research Notes","volume":"18 1","pages":"371"},"PeriodicalIF":1.7,"publicationDate":"2025-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12398087/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144943653","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Exploring the presence of the GPR126 single nucleotide polymorphism (rs536714306) in periodontal patients of European ancestry. 探讨欧洲血统牙周患者GPR126单核苷酸多态性（rs536714306）的存在。

IF 1.7

BMC Research Notes Pub Date : 2025-08-22 DOI: 10.1186/s13104-025-07443-5

Eirini Chatzopoulou, Galinos Fanourakis, Nikolaos Papanikolaou, Ioulia Chatzistamou, Xanthippi Dereka, Heleni Vastardis

{"title":"Exploring the presence of the GPR126 single nucleotide polymorphism (rs536714306) in periodontal patients of European ancestry.","authors":"Eirini Chatzopoulou, Galinos Fanourakis, Nikolaos Papanikolaou, Ioulia Chatzistamou, Xanthippi Dereka, Heleni Vastardis","doi":"10.1186/s13104-025-07443-5","DOIUrl":"https://doi.org/10.1186/s13104-025-07443-5","url":null,"abstract":"Objective: G protein-coupled receptor 126 (GPR126) gene has been implicated as a potential susceptibility factor for aggressive periodontitis in Japanese patients. This study aimed to investigate the presence of the GPR126 [c.3086 G > A] (rs536714306) polymorphism in patients with periodontitis in a Greek population and periodontal cases of European ancestry. A total of 82 subjects were recruited: 53 patients periodontally compromised (P) and 29 healthy controls (H). GPR126 genotyping was performed using Sanger sequencing. Additionally, data from the Gene-Lifestyle Interactions in Dental Endpoints (GLIDE) consortium were included in this study.Results: No variants (rs536714306) in the GPR126 gene were detected in any of the samples. The homozygous for the reference allele GG genotype was observed in 100% of participants across all groups examined. Absence of GPR126 [c.3086 G > A] polymorphism indicates no association with susceptibility to periodontitis in a Greek cohort and periodontally compromised cases of European ancestry. This is the first focused report evaluating the presence of this polymorphism in periodontitis patients in a European population. Further genome-wide studies in larger sample and diverse populations are warranted to fully elucidate the potential role of GPR126 polymorphisms in periodontal disease susceptibility.","PeriodicalId":9234,"journal":{"name":"BMC Research Notes","volume":"18 1","pages":"370"},"PeriodicalIF":1.7,"publicationDate":"2025-08-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12374417/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144943448","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genome sequence of Ralstonia syzygii subsp. celebesensis, the causal agent of banana blood disease on Musa balbisiana cultivar Kepok in bali, Indonesia. 合子拉氏菌亚种基因组序列。印度尼西亚巴厘岛香蕉品种Kepok香蕉血液病病原菌celebesensis。

IF 1.7

BMC Research Notes Pub Date : 2025-08-21 DOI: 10.1186/s13104-025-07398-7

Ady Bayu Prakoso, Siti Subandiyah, Intan Berlian, Tri Joko, Fenny Martha Dwivany, Yudo Prasetyo, Ketut Wikantika, Tri Muji Susantoro

{"title":"Genome sequence of Ralstonia syzygii subsp. celebesensis, the causal agent of banana blood disease on Musa balbisiana cultivar Kepok in bali, Indonesia.","authors":"Ady Bayu Prakoso, Siti Subandiyah, Intan Berlian, Tri Joko, Fenny Martha Dwivany, Yudo Prasetyo, Ketut Wikantika, Tri Muji Susantoro","doi":"10.1186/s13104-025-07398-7","DOIUrl":"https://doi.org/10.1186/s13104-025-07398-7","url":null,"abstract":"Objectives: Blood Disease of Banana brought on by Ralstonia syzygii subsp. celebesensis (Rsc) results in yield losses in Indonesia up to 90%. Among the symptoms are wilting, vascular staining, fruit rot, and plant death. Pathogen travels via contaminated tools, water runoff, pests, and pollinators. Originally reported in South Sulawesi, it now affects all of the main Indonesian islands as well as Malaysia. Though its influence is known, understanding of Rsc pathogenesis is less than that of Ralstonia solanacearum. This study investigates the different genome of Rsc isolate from Bali, geographically separated by the Wallace Line, and compares them to published genomes from Yogyakarta.Data description: The data presented were obtained as a byproduct of a broader genomic survey on Rsc diversity. The genome structure and G + C content analysis of Rsc, which was isolated from Musa balbisiana cultivar Kepok in Bali, is included in the draft genome sequence. The genome's size, coding sequence patterns, and functional annotations were assessed after being assembled from scratch. Genome analysis pipeline was used to process each sequencing data separately using protocols created for Oxford Nanopore sequencing outputs. Comparative analysis with the Yogyakarta isolate highlights molecular variations likely influenced by geographic isolation across the Wallace Line.","PeriodicalId":9234,"journal":{"name":"BMC Research Notes","volume":"18 1","pages":"368"},"PeriodicalIF":1.7,"publicationDate":"2025-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12372176/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144943384","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Computer identification of Notch3 in the neurogenic progenitor cells of mammalian early optic vesicles. 哺乳动物早期视神经泡神经源性祖细胞Notch3的计算机鉴定。

IF 1.7

BMC Research Notes Pub Date : 2025-08-21 DOI: 10.1186/s13104-025-07440-8

Yoshitoku Watabe, Sakurako Kobayashi, Satoru Takahashi, Masaharu Yoshihara

{"title":"Computer identification of Notch3 in the neurogenic progenitor cells of mammalian early optic vesicles.","authors":"Yoshitoku Watabe, Sakurako Kobayashi, Satoru Takahashi, Masaharu Yoshihara","doi":"10.1186/s13104-025-07440-8","DOIUrl":"https://doi.org/10.1186/s13104-025-07440-8","url":null,"abstract":"Objective: The developing mammalian retina initially contains undifferentiated cells, providing a model for investigating the mechanisms of differentiation. Notch signaling, mediated by four Notch receptors (Notch 1-4) in mammals, has been studied in the differentiation of neural progenitor cells. Among the four Notch receptors, the frequency, rather than the peak level, of Notch1-mediated signaling has been suggested to promote the activation of neural progenitor cells. In contrast to Notch1, the involvement of Notch3 in this process is poorly documented, although Notch3 is known for its role in vascular integrity.Results: By re-analyzing publicly available single-cell RNA-seq data from one mouse retinal dataset, two human retinal organoid datasets and two human embryonic retinal datasets, we found that, along with Notch1, Notch3 is expressed in neural progenitor cells in the retina. In addition, the results of the co-expression profile analyses varied among the datasets, leaving uncertainty regarding the regulatory mechanisms of Notch1 and Notch3. Our findings shed light on Notch3 in neurogenic progenitor cells of the developing mammalian retina. Since Notch3 has been suggested to cause ligand-independent signaling, Notch3 expression might antagonize Notch1-mediated signaling oscillations, maintaining the quiescent state of neurogenic progenitor cells.","PeriodicalId":9234,"journal":{"name":"BMC Research Notes","volume":"18 1","pages":"367"},"PeriodicalIF":1.7,"publicationDate":"2025-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12369226/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144943281","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Phenotypic and genotypic relationships between Shigella species and Escherichia coli O37:H10 isolates in Addis Ababa, Ethiopia. 埃塞俄比亚亚的斯亚贝巴志贺氏菌与大肠杆菌O37:H10分离株的表型和基因型关系

IF 1.7

BMC Research Notes Pub Date : 2025-08-21 DOI: 10.1186/s13104-025-07451-5

Basha Ayele, Zeleke Mekonnen, Tesfaye Sisay Tessema, Dawit Hailu Alemayehu, Kalkidan Melaku, Shewki Moga, Maeruf Fetu Nassir, Abaysew Ayele, Adane Mihret, Getenet Beyene

{"title":"Phenotypic and genotypic relationships between Shigella species and Escherichia coli O37:H10 isolates in Addis Ababa, Ethiopia.","authors":"Basha Ayele, Zeleke Mekonnen, Tesfaye Sisay Tessema, Dawit Hailu Alemayehu, Kalkidan Melaku, Shewki Moga, Maeruf Fetu Nassir, Abaysew Ayele, Adane Mihret, Getenet Beyene","doi":"10.1186/s13104-025-07451-5","DOIUrl":"https://doi.org/10.1186/s13104-025-07451-5","url":null,"abstract":"Objective: This study aimed to investigate the genotypic and phenotypic differences between Shigella species and E. coli O37:H10, as well as their antimicrobial resistance (AMR) and virulence factors, in children aged under five with diarrhea in Addis Ababa, Ethiopia.Results: Using whole genome sequencing (WGS), all 28 S. sonnei identified with antisera were also verified as S. sonnei, while all nine isolates of S. flexneri were found to be E. coli O37:H10. One S. flexneri sample, identified serologically, was excluded from WGS analysis because of low genome coverage. Most resistant genes were associated with beta-lactam and trimethoprim drugs. All S. sonnei isolates had genes that encoded blaEC-8 and blaZEG-1. The E. coli O37:H10 had the Extended-spectrum beta-lactamase (ESBL) blaEC-15 for cephalosporins, blaMIX-2, and blaMIX-6 for penicillin. In contrast to S. sonnei, the most prevalent plasmid type in E. coli O37:H10 isolates was IncFII, while virulence genes like gad were frequently detected. Composite transposons, such as cn_43599_IS621 and cn_3785_IS3, were detected in all S. sonnei and E. coli O37:H10. Generally, the study highlighted a significant discrepancy between phenotypic and genotypic AMR, as well as variations in serotypes and phylogenetic relationships with global isolates.","PeriodicalId":9234,"journal":{"name":"BMC Research Notes","volume":"18 1","pages":"369"},"PeriodicalIF":1.7,"publicationDate":"2025-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12372388/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144943585","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0