BMC Research Notes最新文献

{"title":"Sex-specific characteristics of special endurance and performance potential in female runners.","authors":"Wolfgang Blödorn, Frank Döring","doi":"10.1186/s13104-025-07256-6","DOIUrl":"https://doi.org/10.1186/s13104-025-07256-6","url":null,"abstract":"<p><strong>Objective: </strong>The coefficient of special endurance (KsA) is a metric that quantifies the relative pace loss between two consecutive distances (e.g., 100 m/200m). Here, we analyzed over 20,000 race times to determine KsA values for female runners across seven distance pairs from 100 m to 10,000 m. The data analyses are based on multiple official performance rankings at international to regional levels, exclusively compiled and processed for this study.</p><p><strong>Results: </strong>The KsA values obtained have remained stable for over four decades in national-level female runners and are applicable from world-class to regional levels. A sex-based analysis reveals that females undergo a more pronounced decrease in pace from 100 m to 1500 m in comparison to males. These sex differences in special endurance align with known variations in muscle fiber composition and fast-twitch type II fiber characteristics between males and females. In conclusion, we provide statistically valid KsA reference values for female runners from 100 m to 10,000 m. These values have practical implications for coaches and athletes seeking to assess runners' strengths, weaknesses, potential, and specific talents based on race times. Sex differences in KsA values may reflect muscle physiology and guide future research on KsA and muscle function.</p>","PeriodicalId":9234,"journal":{"name":"BMC Research Notes","volume":"18 1","pages":"196"},"PeriodicalIF":1.6,"publicationDate":"2025-04-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12032771/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143980845","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The influence of the geroprotective cytokine on the transcriptome of young and senescent mesenchymal stem cells.","authors":"Liya G Kondratyeva, Diana K Matveeva, Maria I Ezdakova, Marina V Utkina, Andrey Yu Ratushnyy","doi":"10.1186/s13104-025-07262-8","DOIUrl":"https://doi.org/10.1186/s13104-025-07262-8","url":null,"abstract":"<p><strong>Objectives: </strong>Increasing longevity and the growing elderly population necessitate a deeper understanding of aging mechanisms to prolong productive life and improve treatments for age-related diseases linked with cellular senescence. Mesenchymal stem cells (MSCs) are crucial for maintaining tissue homeostasis, but their physiological changes during senescence are not well understood. Growth differentiation factor 11 (GDF11) has emerged as a potential rejuvenation factor, enhancing MSC viability, mobility, and angiogenic functions, which improves outcomes in ischemic models and cardiac repair. This study aims to identify transcriptomic changes in young and senescent MSCs influenced by GDF11, highlighting its potential in MSC-based therapies.</p><p><strong>Data description: </strong>To evaluate transcriptomic changes induced by the potential geroprotective factor GDF11, we performed RNA sequencing on four groups of samples: 'young' MSCs (MmC-/GDF11-) and senescent MSCs (MmC+/GDF11-) without the addition of GDF11, as well as 'young' (MmC-/GDF11+) and senescent MSCs (MmC+/GDF11+) with the addition of GDF11. After 10 days of incubation, indexed cDNA libraries for Illumina sequencing were prepared from the samples, and the resulting cDNA library mix was subjected to NovaSeq 6000 sequencing. This paper describes the collection of 16 RNA sequencing samples comprising 4 sets of MSCs. FASTQ files from Illumina sequencing are available in the NCBI Gene Expression Omnibus.</p>","PeriodicalId":9234,"journal":{"name":"BMC Research Notes","volume":"18 1","pages":"195"},"PeriodicalIF":1.6,"publicationDate":"2025-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12023522/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143959723","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparison of methods of optimal cut-point selection for biomarkers in diagnostic medicine: a simulation study with application of clinical data in health informatics.","authors":"Mojtaba Hassanzad, Karimollah Hajian-Tilaki, Zinatossadat Bouzari, Shahla Yazdani","doi":"10.1186/s13104-025-07245-9","DOIUrl":"https://doi.org/10.1186/s13104-025-07245-9","url":null,"abstract":"<p><strong>Objectives: </strong>Several methods of cut-point selection for biomarkers have been suggested in biomedical research but the superiority of them over others was not studied comprehensively under different pairs of distributions, degree of overlap, and the ratio of sample sizes. This simulation study was aimed to compare five popular methods with application of clinical examples.</p><p><strong>Results: </strong>The data of simulation was generated from the 12 configurations of binormal, bigamma, and biexponential pairs with different sample sizes The results showed that the four popular methods of Youden, Euclidean, Product, and Index of Union (IU) yielded identical optimal cut-point under binormal model with homoscedastic. While, with high AUC, the Youden may produce less bias and MSE, but for moderate and low AUC, Euclidean has less bias and MSE than other methods. The IU yielded more precise findings than the Youden for moderate and low AUC in binormal pairs, but its performance was lower with skewed distributions. In contrast, the cut-points produced by diagnostic odds ratio (DOR) were extremely high with low sensitivity and high MSE and bias. The results of clinical data showed that when AUC > 0.95, the five methods may produce identical cut-point, but DOR yields an extremely high value of cut-point for AUC < 0.95.</p>","PeriodicalId":9234,"journal":{"name":"BMC Research Notes","volume":"18 1","pages":"193"},"PeriodicalIF":1.6,"publicationDate":"2025-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12020263/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143981307","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Thermal potentiation improves IFN-γ production but not cytotoxicity in human CAR-T cells.","authors":"Niladri Bhusan Pati, Yixin Jin, Suman Kumar, Jon Amund Kyte, Rafal Ciosk","doi":"10.1186/s13104-025-07249-5","DOIUrl":"https://doi.org/10.1186/s13104-025-07249-5","url":null,"abstract":"<p><strong>Objective: </strong>Body temperature plays an important role in cancer, with febrile temperature generally associated with improved cancer resistance. In murine models, this resistance has been linked to the cytotoxic T cells, whose differentiation into cancer-killing effector cells is poor at lower but robust at elevated temperatures. If conserved, temperature-mediated potentiation of patient-derived T cells could be implemented to improve the existing cancer treatments, including the chimeric antigen receptor T-cell therapy (CAR T-cell therapy). Here, we tested this possibility using human STEAP1 CAR-T cells developed to target prostate cancer.</p><p><strong>Results: </strong>In mice, transient exposure to febrile temperature (39-40 ºC) increases the production of IFN-γ and the cancer-killing ability of CD8 + T cells. Using a similar temperature treatment, we observed elevated levels of IFN-γ also in the human CAR-T cells. However, these cells displayed no improvement in their ability to kill cancer cells. Although we cannot discount the possibility that alternative protocols might lead to other outcomes, our findings suggest that incorporating thermal potentiation into existing protocols of CAR-T cell therapy may be more complicated than anticipated.</p>","PeriodicalId":9234,"journal":{"name":"BMC Research Notes","volume":"18 1","pages":"192"},"PeriodicalIF":1.6,"publicationDate":"2025-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12020131/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143976823","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Using LDpred2 to adapt polygenic risk score techniques for methylation score creation.","authors":"Kristoffer Sandås, Leticia Spindola, Solveig Løkhammer, Anne-Kristin Stavrum, Ole Andreassen, Markos Tesfaye, Stéphanie Le Hellard","doi":"10.1186/s13104-025-07222-2","DOIUrl":"https://doi.org/10.1186/s13104-025-07222-2","url":null,"abstract":"<p><strong>Objective: </strong>This study sought to determine if the R package LDpred2, designed for polygenic risk score creation for genome-wide association studies using summary statistics, could be adapted for deriving DNA methylation scores from methylome-wide association studies. Recognizing that linkage disequilibrium, used as prior in LDpred2, does not apply to methylation, we explored co-methylated regions and topologically associating domains as alternative structural priors for correlation between methylation sites. A genomic sliding-window approach was also tested. The performance of the LDpred2-based models was evaluated on methylation data from schizophrenia and control samples (N = 1,227).</p><p><strong>Results: </strong>LDpred2 models employing topologically associating domains and sliding window clusters as priors performed similarly to existing methods, explaining approximately 3.6% of schizophrenia phenotypic variance. The co-methylated regions model underperformed due to insufficient clustering of probes. The similarity in performance between the model using topologically associating domains and a null model consisting of random clusters suggests that the structural information provided by these domains enhances performance only marginally. In conclusion, while LDpred2 can be adapted for methylation data, it does not substantially enhance methylation score performance over existing methods, and the choice of structural prior may not be a critical factor.</p>","PeriodicalId":9234,"journal":{"name":"BMC Research Notes","volume":"18 1","pages":"190"},"PeriodicalIF":1.6,"publicationDate":"2025-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12016360/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143989022","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comprehensive single-cell RNA-sequencing study of Tollip deficiency effect in IL-13-stimulated human airway epithelial cells.","authors":"Grace Yihua Lee, Niccolette Schaunaman, Hamid Reza Nouri, Monica Kraft, Hong Wei Chu","doi":"10.1186/s13104-025-07255-7","DOIUrl":"https://doi.org/10.1186/s13104-025-07255-7","url":null,"abstract":"<p><strong>Objective: </strong>Toll-interacting protein (Tollip) suppresses excessive pro-inflammatory signaling, but its function in airway epithelial responses to IL-13, a key mediator in allergic diseases, remains unclear. This study investigates Tollip knockdown (TKD) effects in primary human airway epithelial cells using single-cell RNA sequencing, providing the first single-cell analysis of TKD and the first exploring its interaction with IL-13.</p><p><strong>Results: </strong>IL-13 treatment upregulated key genes, including SPDEF, MUC5AC, POSTN, ALOX15, and CCL26, confirming IL-13's effects and validating our methods. IL-13 reduced TNF-α signaling and epithelial-mesenchymal transition in certain cell types, suggesting a dual role in promoting type 2 inflammation while suppressing Th1-driven inflammation. Tollip deficiency alone significantly amplified TNF-α signaling and inflammatory pathways in goblet, club, and suprabasal cells. Comparisons between TKDIL13 vs IL13 and TKD vs CTR revealed that IL-13 does not substantially alter Tollip deficiency response in most cell types, reinforcing findings in TKD vs CTR. Tollip deficiency alters the response to IL-13 in a cell-type-specific manner, strongly downregulating TNF-α signaling in goblet cells but only weakly in basal and club cells. Tollip deficiency enhances IL-13's suppression of Th1 inflammatory responses in goblet cells. These novel insights in Tollip-IL-13 interactions offer potential therapeutic targets for asthma and related diseases.</p>","PeriodicalId":9234,"journal":{"name":"BMC Research Notes","volume":"18 1","pages":"194"},"PeriodicalIF":1.6,"publicationDate":"2025-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12020103/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143980795","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of the price-efficacy relationship for multiple brands of ciprofloxacin and gentamicin in Kabul: a cross-sectional study.","authors":"Ahmad Zia Noori, Hashmatullah Yousufi, Haji Mohammad Naimi","doi":"10.1186/s13104-025-07258-4","DOIUrl":"https://doi.org/10.1186/s13104-025-07258-4","url":null,"abstract":"<p><strong>Background: </strong>The quality of consumer products constitutes a prominent issue on a global scale. The proliferation of counterfeit pharmaceuticals poses a significant challenge not only in developing and underdeveloped nations but also represents a considerable concern in developed countries. In Kabul, a major issue is the availability of multiple brands of antibiotics with fluctuating prices. To ascertain the quality and correlation of pricing with the efficacy of antibiotics, an evaluation of their effectiveness is deemed both essential and significant. Ciprofloxacin and Gentamicin are widely utilized antibiotics for treating infections induced by specific strains of Gram-negative and Gram-positive bacteria. The aim of this study was to investigate the relationship between brand price and the efficacy of these antibiotics.</p><p><strong>Methods: </strong>A total of 40 ciprofloxacin 500 mg tablet brands and 15 gentamicin 80 mg ampule brands were procured from pharmacies in Kabul across eight countries. Ten samples from each brands were assessed for efficacy through MIC and MBC assays against Staphylococcus aureus (ATCC 29213), following Clinical and Laboratory Standards Institute protocols. Efficacy data were obtained by inoculating S. aureus suspensions in Mueller-Hinton medium with various concentrations of each antibiotic, incubated at 35-37˚C for 24 h. Following MIC determination, inoculated cultures were plated on Mueller-Hinton agar and incubated at 35-37˚C for 24 h for colony enumeration. Statistical analysis was conducted using ANOVA, Student's t-test, and Pearson correlation via SPSS version 26, with p-values of ≤ 0.05 considered significant.</p><p><strong>Results: </strong>The prices of Ciprofloxacin tablets ranged from 25 to 275 Afghanis (mean = 99.60), while Gentamicin prices varied from 3 to 15 Afghanis (mean = 8.37). Of the 40 ciprofloxacin brands, 6 (15%) were unregistered, and from 15 gentamicin brands, 3 (20%) were unregistered. There was no significant difference in Gentamicin efficacy against S. aureus. However, a significant difference was noted in Ciprofloxacin efficacy against S. aureus (p < 0.01).</p><p><strong>Conclusions: </strong>The results of this study highlight the critical need for stringent quality control and regulatory oversight, especially for unregistered antibiotics, due to the significant variations observed in Ciprofloxacin efficacy (p < 0.01), even though no such difference was noted with Gentamicin. Policymakers should implement regulations to ensure all antibiotic brands adhere to quality standards, while pharmacists and healthcare professionals should prioritize using registered, effective medications to safeguard public health.</p>","PeriodicalId":9234,"journal":{"name":"BMC Research Notes","volume":"18 1","pages":"189"},"PeriodicalIF":1.6,"publicationDate":"2025-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12016291/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143966150","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nursing students' attitudes toward cross-gender care: a cross-sectional study.","authors":"Saumya P Srivastava, Soni Chauhan, Anuj Singh, Surya Kant Tiwari, Surat Ram Kudi, Anchal Gupta","doi":"10.1186/s13104-025-07254-8","DOIUrl":"https://doi.org/10.1186/s13104-025-07254-8","url":null,"abstract":"<p><strong>Objectives: </strong>This study aimed to assess nursing students' attitudes toward providing cross-gender care and to identify the factors influencing these attitudes.</p><p><strong>Results: </strong>A cross-sectional study of 338 nursing students in Northern India found that over half (50.9%) had unfavorable attitudes towards cross-gender care. Female students demonstrated significantly more positive attitudes than male students (p < 0.01). Multiple regression analysis identified gender (β = 0.246, p < 0.001), academic year (β=-0.150, p = 0.009), and prior experience with cross-gender care (β = 0.100, p = 0.048) as significant predictors of attitudes. The regression model explained 19.9% of the variance in attitudes (R²=0.199, p < 0.001). Male students expressed concerns about providing quality physical care and emotional support for patients and felt inadequately prepared to provide physical and emotional support to opposite-gender patients. These findings highlight the need for enhanced gender-sensitive training in nursing education to improve attitudes and competencies in cross-gender care provision.</p>","PeriodicalId":9234,"journal":{"name":"BMC Research Notes","volume":"18 1","pages":"191"},"PeriodicalIF":1.6,"publicationDate":"2025-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12020158/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143980798","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Food and nutrition security of adolescents and young adults NEETs and non-NEETs in two low socioeconomic urban settlements in South Africa.","authors":"Sicelosethu S Siro, Nazeeia Sayed, Elizabeth Catherina Swart","doi":"10.1186/s13104-025-07257-5","DOIUrl":"https://doi.org/10.1186/s13104-025-07257-5","url":null,"abstract":"<p><strong>Introduction: </strong>Adolescents and young adults not yet in employment, education, or training (NEETs) account for a sizable fraction of the global population. In South Africa, 34.3% of young individuals aged 15 to 24 were classified as NEETs in 2022. Lack of work in low-income areas may lead to poverty and food insecurity, negatively impacting diet quality.</p><p><strong>Objective: </strong>This cross-sectional study aimed to evaluate and compare the anthropometric status, dietary intake, and food security of NEET and non-NEET adolescents and young adults (aged 18-24 years) in Langa and Fisantekraal, two low socioeconomic settlements in South Africa.</p><p><strong>Methods: </strong>Participants were recruited through door-to-door visits. Standardized instruments including a socio-demographic questionnaire, household hunger score, lived poverty index, anthropometric assessments, and 24-hour dietary recalls were used for data collection.</p><p><strong>Results: </strong>There were no significant differences in the prevalence of household hunger (p = 0.496), the lived poverty index (p = 0.111) and the prevalence of low micronutrient intakes between the NEETS and non-NEETs (p > 0.05). The prevalence of inadequate daily iron intake had the lowest prevalence while the prevalence of inadequate calcium intake was highest in this group of adolescents and young adults. The insufficient intake of micronutrients among adolescents and young adults can be attributed to the diets consumed by this demographic group.</p>","PeriodicalId":9234,"journal":{"name":"BMC Research Notes","volume":"18 1","pages":"187"},"PeriodicalIF":1.6,"publicationDate":"2025-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12016356/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143965854","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optimization of mucilage secretion of Plantago ovata, Alyssum desertorum, and Ocimum basilicum seeds by response surface method.","authors":"Bahram Tafaghodinia","doi":"10.1186/s13104-023-06517-6","DOIUrl":"https://doi.org/10.1186/s13104-023-06517-6","url":null,"abstract":"<p><p>Diaspore comprises a collection of seed coats and other organ components that effectively protect the embryo and dispersal. The mucilage in many species, a gelatinous mixture of cell wall polysaccharides, is secreted by the pericarp. Although the modification, synthesis, and secretion of the strain of mucilage as well as structure and roles of plant cell wall have been of great interest and study by researchers, the understanding of the best conditions for the secretion of mucilage has received low attention. This research aimed to investigate effective factors in the process of mucilage secretion in P. ovata, A. desertorum, and O. basilicum seeds. To achieve this objective, an investigation was conducted to examine the impact of seed number, polarity, pH and species on mucilage secretion. This study showed that the number of seeds and type of species had the least and the most effect on mucilage production, respectively. Finally, according to the results of Response Surface Method design experiments, pH, polarity and species had a significant effect on the process of mucilage secretion. pH = 4 and polarity = 15 for P. ovata and pH = 10 and polarity = 15 for O. basilicum and A. desertorum were the most favorable conditions for secretion of 10.3 mm, 9.4 mm, and 2.9 mm of mucilage, respectively.</p>","PeriodicalId":9234,"journal":{"name":"BMC Research Notes","volume":"18 1","pages":"188"},"PeriodicalIF":1.6,"publicationDate":"2025-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12016475/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143973887","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}