BMC Research Notes最新文献_第6页

The M91 metallopeptidase domain of the T6SS effector protein SED_RS06335 encoded in Salmonella Dublin SPI-19 exhibits antibacterial activity. 沙门氏菌都柏林SPI-19编码的T6SS效应蛋白SED_RS06335的M91金属肽酶结构域具有抗菌活性。

IF 1.7

BMC Research Notes Pub Date : 2026-06-25 DOI: 10.1186/s13104-026-07931-2

Carla Vargas-Del Río, Ayleen Parra-Calisto, Carlos J Blondel, Felipe Reyes-Méndez, Fernando A Amaya, Carlos A Santiviago, Andrea Avilés, Victoria Soriano-Mora, Daniel Chacín, David Pezoa

{"title":"The M91 metallopeptidase domain of the T6SS effector protein SED_RS06335 encoded in Salmonella Dublin SPI-19 exhibits antibacterial activity.","authors":"Carla Vargas-Del Río, Ayleen Parra-Calisto, Carlos J Blondel, Felipe Reyes-Méndez, Fernando A Amaya, Carlos A Santiviago, Andrea Avilés, Victoria Soriano-Mora, Daniel Chacín, David Pezoa","doi":"10.1186/s13104-026-07931-2","DOIUrl":"10.1186/s13104-026-07931-2","url":null,"abstract":"Objective: The Type VI Secretion System (T6SS) is a contractile apparatus made of several proteins playing a significant role in the fitness and virulence of many Gram-negative bacteria. The SPI-19 T6SS gene cluster is a virulence factor of Salmonella Dublin contributing to host colonization and antibacterial activity. Previously, we demonstrated that SED_RS06335 (an Rhs protein with a C-terminal M91 domain) is responsible for antibacterial activity of the T6SSSPI-19; however, the role of the M91 domain in this phenotype is unknown. Thus, the objective of this study is to provide experimental evidence that the antibacterial activity of SED_RS06335 effector is attributable to its C-terminal M91 domain.Results: Here, we determine through interbacterial competition and heterologous expression assays that M91 domain of SED_RS06335 displays antibacterial activity. Furthermore, a three-dimensional structural model of M91 domain revealed a high similarity with the metallopeptidase neurotoxin type A (BotA) from Clostridium botulinum. Interestingly, our results suggest that the M91 domain is responsible for the antibacterial activity of the SED_RS06335 effector in Salmonella Dublin.","PeriodicalId":9234,"journal":{"name":"BMC Research Notes","volume":" ","pages":""},"PeriodicalIF":1.7,"publicationDate":"2026-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13556075/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148326893","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sodium selenate stimulates growth and photosynthetic pigments in sweet potato plantlets in vitro. 硒酸钠对红薯试管苗生长和光合色素的影响。

IF 1.7

BMC Research Notes Pub Date : 2026-06-24 DOI: 10.1186/s13104-026-07917-0

Marcelo de Almeida Guimarães, Jorge Braga Ribeiro Junior, Filipe Almendagna Rodrigues, Lailla Sabrina Queiroz Nazareno, Moacir Pasqual, Gabrielen de Maria Gomes Dias, Helon Hébano de Freitas Sousa

{"title":"Sodium selenate stimulates growth and photosynthetic pigments in sweet potato plantlets in vitro.","authors":"Marcelo de Almeida Guimarães, Jorge Braga Ribeiro Junior, Filipe Almendagna Rodrigues, Lailla Sabrina Queiroz Nazareno, Moacir Pasqual, Gabrielen de Maria Gomes Dias, Helon Hébano de Freitas Sousa","doi":"10.1186/s13104-026-07917-0","DOIUrl":"10.1186/s13104-026-07917-0","url":null,"abstract":"Objective: Selenium deficiency is a widespread nutritional problem worldwide, and biofortification has been proposed as a strategy to increase selenium intake through food crops. This study evaluated the effects of sodium selenate on growth, photosynthetic pigments, and selenium accumulation in sweet potato (Ipomoea batatas) plantlets micropropagated in vitro.Results: Explants were cultured in hormone-free Murashige and Skoog medium supplemented with 0, 5, 10, 15, 20, and 25 µM sodium selenate. Plantlets grown with 5.0 µM sodium selenate showed the highest values for growth-related traits, including number of leaves, shoot length, root length, and dry biomass. Photosynthetic pigments were also enhanced at low selenium concentrations, with maximum chlorophyll and carotenoid contents observed at 5.0 µM. In contrast, higher selenium concentrations reduced plant growth and pigment accumulation, indicating phytotoxic effects. Selenium accumulation increased progressively with increasing sodium selenate concentration, reaching the highest levels at 25.0 µM. These findings indicate that 5.0 µM sodium selenate is suitable for in vitro propagation of sweet potato plantlets, whereas higher concentrations may be useful for initiating selenium biofortification strategies.","PeriodicalId":9234,"journal":{"name":"BMC Research Notes","volume":" ","pages":""},"PeriodicalIF":1.7,"publicationDate":"2026-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13551956/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148315910","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Association of IBS-associated E. coli strains with epithelial inflammatory responses and tight junction dysregulation in Caco-2 cells. 肠易激相关大肠杆菌菌株与上皮炎症反应和Caco-2细胞紧密连接失调的关系

IF 1.7

BMC Research Notes Pub Date : 2026-06-23 DOI: 10.1186/s13104-026-07924-1

Zeinab Merzaei, Hossein Kazemian, Zahra Bamzadeh, Mansoureh Paknejadi, Ebrahim Kouhsari

引用次数: 0

Celecoxib modulates inflammatory and immunoregulatory gene expression in human monocyte-derived dendritic cells. 塞来昔布调节人类单核细胞来源的树突状细胞中的炎症和免疫调节基因表达。

IF 1.7

BMC Research Notes Pub Date : 2026-06-22 DOI: 10.1186/s13104-026-07921-4

Vida Hashemi, Behzad Baradaran, Bahar Naseri, Javad Masoumi, Elham Baghbani, Nazila Alizadeh, Mahya Zolfaghar Ghoshooni, Arezoo Hosseini

{"title":"Celecoxib modulates inflammatory and immunoregulatory gene expression in human monocyte-derived dendritic cells.","authors":"Vida Hashemi, Behzad Baradaran, Bahar Naseri, Javad Masoumi, Elham Baghbani, Nazila Alizadeh, Mahya Zolfaghar Ghoshooni, Arezoo Hosseini","doi":"10.1186/s13104-026-07921-4","DOIUrl":"10.1186/s13104-026-07921-4","url":null,"abstract":"Dendritic cells (DCs) play a pivotal role in regulating immune responses, influencing both pro-inflammatory and anti-inflammatory environments. Given the critical role of the COX-2/PEG2 pathway in regulating DC maturation and cytokine production, we investigated whether selective COX-2 inhibition by celecoxib could shift human monocyte-derived dendritic cells (mDCs) toward a toerogenic profile. The impact of celecoxib treatment on the expression of various inflammatory and anti-inflammatory factors, including IL-12, TNF-α, IL-10, IDO, TGF-β, and STAT3, was evaluated. Our results demonstrate that the expression of key anti-inflammatory factors was enhanced in DCs, suggesting that COX-2 inhibition could promote a tolerogenic phenotype. In conclusion, the therapeutic modulation of DCs through COX-2 inhibition could have applications beyond autoimmune diseases, offering potential for broader immunomodulatory therapies. Further studies are required to validate these findings and explore clinical applications.","PeriodicalId":9234,"journal":{"name":"BMC Research Notes","volume":" ","pages":""},"PeriodicalIF":1.7,"publicationDate":"2026-06-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13536593/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148293417","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Widespread adhesion and iron acquisition traits in Escherichia coli strains obtained from irritable bowel syndrome (IBS) patients. 肠易激综合征（IBS）患者大肠杆菌菌株的广泛粘附和铁获取特性

IF 1.7

BMC Research Notes Pub Date : 2026-06-22 DOI: 10.1186/s13104-026-07922-3

Arezo Khadiv, Mohammad Yazdanmanesh, Hamid Heidari, Fateme Yazdani, Hamidreza Houri, Behzad Badakhsh, Sobhan Ghafourian, Hossein Kazemian

{"title":"Widespread adhesion and iron acquisition traits in Escherichia coli strains obtained from irritable bowel syndrome (IBS) patients.","authors":"Arezo Khadiv, Mohammad Yazdanmanesh, Hamid Heidari, Fateme Yazdani, Hamidreza Houri, Behzad Badakhsh, Sobhan Ghafourian, Hossein Kazemian","doi":"10.1186/s13104-026-07922-3","DOIUrl":"10.1186/s13104-026-07922-3","url":null,"abstract":"Background: The intestinal microbiome is essential for maintaining a balanced and healthy gut environment. Certain Escherichia coli strains can disrupt it through various pathogenic factors and have been linked to gastrointestinal diseases. In this study, E. coli strains were isolated from individuals with irritable bowel syndrome (IBS) and healthy controls. Identification was performed using microbiological and molecular methods. The presence of key virulence factors, including adhesins (iha, lpfA, afaC, sfaDE, papC, focG, aafII), iron acquisition systems (chuA, iroN, fepC, irp2, iutA, ireA), hemolysin (hlyA), microcins and colicins (cva, colY), and multifunctional factors (malX, yajQ), was assessed by polymerase chain reaction.Results: The genes lpfA, papC, iroN, ireA, cva, and malX were found to be significantly more prevalent in IBS-associated isolates following Bonferroni correction (p < 0.0031). A higher prevalence of sfaDE, focG, chuA, and hlyA was also observed, though these differences were not statistically significant. Conversely, the genes iha and irp2 were significantly more common in E. coli isolates from healthy individuals. Notably, none of the isolates harbored the afaC and aafII genes. This study demonstrates significant differences in the distribution of specific virulence genes between E. coli isolates from IBS patients and healthy individuals. The higher prevalence of specific genes in IBS isolates may contribute to their pathogenic potential, whereas the frequent occurrence of iha and irp2 in healthy individuals may suggest a role in maintaining a balanced gut microbiome. These findings highlight distinct virulence gene profiles, suggesting a potential association between these E. coli factors and IBS disease status.","PeriodicalId":9234,"journal":{"name":"BMC Research Notes","volume":" ","pages":""},"PeriodicalIF":1.7,"publicationDate":"2026-06-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13540835/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148300957","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Monitoring infestation levels using an AI-based Chironomid quantification tool (CQT) in a laboratory Xenopus laevis facility. 在实验室非洲爪蟾设施中使用基于人工智能的摇尾虫定量工具（CQT）监测虫害水平。

IF 1.7

BMC Research Notes Pub Date : 2026-06-19 DOI: 10.1186/s13104-026-07919-y

Maximilian Schwarzbach, Bastian Popper

{"title":"Monitoring infestation levels using an AI-based Chironomid quantification tool (CQT) in a laboratory Xenopus laevis facility.","authors":"Maximilian Schwarzbach, Bastian Popper","doi":"10.1186/s13104-026-07919-y","DOIUrl":"10.1186/s13104-026-07919-y","url":null,"abstract":"Objective: Insect infestations in laboratory animal facilities represent a potential risk to animal welfare, staff hygiene, and experimental integrity. In this study, we developed and applied a custom AI-assisted image analysis workflow, the Chironomid Quantification Tool (CQT-AI), to detect and quantify infestations of non-biting midges (Chironomidae) in an aquatic housing system for Xenopus laevis. To evaluate the applicability of CQT-AI for monitoring infestation dynamics and treatment effects, different control strategies, including increased water exchange and biological control using Bacillus thuringiensis israelensis (Bti), were tested in parallel.Results: CQT-AI proved to be a reliable tool for detecting and quantifying infestation dynamics of adult Chironomidae, enabling objective assessment of treatment effects within 1 min. Increased water exchange resulted in measurable changes in water chemistry but only moderately reduced infestation levels. In contrast, treatment with Bti led to rapid and near-complete eradication of adult midges within six weeks without detectable adverse effects on animal health. Overall, the study demonstrates that CQT-AI provides an efficient and objective approach for monitoring infestation dynamics and evaluating control strategies in aquatic laboratory systems.","PeriodicalId":9234,"journal":{"name":"BMC Research Notes","volume":" ","pages":""},"PeriodicalIF":1.7,"publicationDate":"2026-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13340404/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148283308","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Adaptive cargo deletion as an evolutionary dead-end during phage-based directed evolution. 基于噬菌体的定向进化中适应性货物缺失是进化的死胡同。

IF 1.7

BMC Research Notes Pub Date : 2026-06-19 DOI: 10.1186/s13104-026-07913-4

Zidan Li, Ibrahim Al'Abri, Nathan Crook

{"title":"Adaptive cargo deletion as an evolutionary dead-end during phage-based directed evolution.","authors":"Zidan Li, Ibrahim Al'Abri, Nathan Crook","doi":"10.1186/s13104-026-07913-4","DOIUrl":"10.1186/s13104-026-07913-4","url":null,"abstract":"Objective: The purpose of this project was to evaluate the performance and reliability of the P1 phage-based inducible directed evolution (IDE) method for performing global transcriptional machinery engineering (gTME) to improve pH tolerance in Escherichia coli Nissle. Unlike prior gTME approaches that evolved single sigma factors, we simultaneously mutated three sigma factors associated with pH tolerance (rpoD, rpoN, and rpoH) through five rounds of mutagenesis and growth-based selection at pH 4.2.Results: Mutations in rpoD (V98E and R603C) emerged during early rounds and reduced the lag phase under acidic conditions. However, library improvement plateaued after the third cycle. In later rounds, an empty vector resulting from the deletion of plasmid-encoded sigma factors became dominant. This deletion of plasmid-encoded sigma factors improved low-pH fitness, but it prevented further mutations and led to an evolutionary dead end. Additionally, transcriptome analysis showed that the single mutations (V98E, R603C) that preceded sigma factor deletion improved fitness through mechanisms resembling those observed upon sigma factor deletion. Together, these findings indicate that phage-based and continuous directed evolution approaches may face challenges if the evolutionary substrate itself reduces performance relative to a wild-type strain.","PeriodicalId":9234,"journal":{"name":"BMC Research Notes","volume":" ","pages":""},"PeriodicalIF":1.7,"publicationDate":"2026-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13528150/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148283353","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Hydroxyurea interferes with point-of-care creatinine testing in children with sickle cell anemia. 羟基脲干扰镰状细胞性贫血患儿的即时肌酸酐检测。

IF 1.7

BMC Research Notes Pub Date : 2026-06-19 DOI: 10.1186/s13104-026-07916-1

Ruth Namazzi, Robert O Opoka, Charles Ssuuna, Michael J Goings, Kathryn McElhinney, Dibyadyuti Datta, Russell E Ware, Chandy C John, Andrea L Conroy

{"title":"Hydroxyurea interferes with point-of-care creatinine testing in children with sickle cell anemia.","authors":"Ruth Namazzi, Robert O Opoka, Charles Ssuuna, Michael J Goings, Kathryn McElhinney, Dibyadyuti Datta, Russell E Ware, Chandy C John, Andrea L Conroy","doi":"10.1186/s13104-026-07916-1","DOIUrl":"10.1186/s13104-026-07916-1","url":null,"abstract":"Objective: To evaluate the performance of the point-of-care i-STAT device for creatinine measurement in Ugandan children with sickle cell anemia (SCA), and to determine the clinical impact of hydroxyurea-associated assay interference.Results description: This secondary analysis was nested within a randomized clinical trial involving 248 Ugandan children with SCA. The mean age at enrollment was 32 months, and 115/248 (46.2%) initiated hydroxyurea during 12 months of follow-up as part of routine clinical care. At enrollment, creatinine values measured by i-STAT and reference laboratory testing were clinically comparable. At follow-up, children receiving hydroxyurea had significantly higher creatinine values measured by i-STAT than by reference laboratory testing (mean difference + 0.19 mg/dL; p = 0.006), with several i-STAT results falling within the clinically abnormal range. No such discrepancy was observed among children not receiving hydroxyurea. In a subset of samples, serum hydroxyurea concentrations measured by high-performance liquid chromatography accounted for 73% of the variability in i-STAT creatinine values. These findings demonstrate clinically meaningful interference of hydroxyurea with enzymatic point-of-care creatinine testing, which may result in misclassification of kidney function and inappropriate clinical decision-making in children with SCA receiving hydroxyurea.Clinicaltrials: gov identifier: NCT03528434 (date registered: May 7th, 2018).","PeriodicalId":9234,"journal":{"name":"BMC Research Notes","volume":" ","pages":""},"PeriodicalIF":1.7,"publicationDate":"2026-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13523223/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148283340","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dysregulation of GDNF and GFRα1 expression in multifactorial Hirschsprung disease. 多因子巨结肠疾病中GDNF和GFRα1表达异常

IF 1.7

BMC Research Notes Pub Date : 2026-06-19 DOI: 10.1186/s13104-026-07912-5

Gunadi, Setiani Silvy Nurhidayah, Aleyy Adany, Farah Shahnaz Pravinovia, Siti Maisaroh, Kurnia Corie Tonda, Pramana Adhityo, Estelita Liana, Kristy Iskandar, Andi Dwihantoro

{"title":"Dysregulation of GDNF and GFRα1 expression in multifactorial Hirschsprung disease.","authors":"Gunadi, Setiani Silvy Nurhidayah, Aleyy Adany, Farah Shahnaz Pravinovia, Siti Maisaroh, Kurnia Corie Tonda, Pramana Adhityo, Estelita Liana, Kristy Iskandar, Andi Dwihantoro","doi":"10.1186/s13104-026-07912-5","DOIUrl":"10.1186/s13104-026-07912-5","url":null,"abstract":"Objective: HSCR is caused by disruption of complex signaling pathways within the gene regulatory network (GRN) during enteric nervous system (ENS) development, including glial cell line-derived neurotrophic factor (GDNF) and GDNF family receptor alpha-1 (GFRα1). However, pathogenic variants in all GRN genes account for only ~ 80% cases; therefore, the epigenetic role remains to be elucidated. We compared GDNF and GFRα1 expression between HSCR patients and controls.Results: qPCR revealed an upregulated GDNF expression in both ganglionic (206.37-fold) and aganglionic (126.35-fold) HSCR compared to control colons (ΔCT 7.65 ± 2.19 vs. 15.34 ± 1.34; p = 0.0001; and ΔCT 8.35 ± 2.57 vs. 15.34 ± 1.34; p = 0.0001). qPCR also showed an upregulated GFRα1 expression in both ganglionic (29.66-fold) and aganglionic (18.44-fold) HSCR compared to control colons (ΔCT 8.44 ± 1.98 vs. 13.33 ± 1.36; p = 0.0001; and ΔCT 9.13 ± 1.46 vs. 13.33 ± 1.36; p = 0.0001). In addition, no significant differences were observed between ganglionic and aganglionic segments for either GDNF or GFRα1 (ΔCT 7.65 ± 2.19 vs. 8.35 ± 2.57; p = 0.43; and ΔCT 8.44 ± 1.98 vs. 9.13 ± 1.46; p = 0.29). Our study demonstrates global aberrant expression of GDNF and GFRα1 in HSCR patients. These findings underscore the complexity of HSCR as a multifactorial disorder and highlight the importance of integrated signaling networks in ENS development, providing a rationale for further mechanistic and translational studies.","PeriodicalId":9234,"journal":{"name":"BMC Research Notes","volume":" ","pages":""},"PeriodicalIF":1.7,"publicationDate":"2026-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13528072/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148283317","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ecological association between Grindr user characteristics and HIV prevalence in Malaysia: a cross-sectional study. Grindr用户特征与马来西亚艾滋病毒流行之间的生态关联：一项横断面研究。

IF 1.7

BMC Research Notes Pub Date : 2026-06-19 DOI: 10.1186/s13104-026-07920-5

Hakimi Kassim, Kamarul Ismail, Md Enamul Huq, Hein Htet Aung, Ethan Trey Cardwell, Fabian Yuh Shiong Kong

{"title":"Ecological association between Grindr user characteristics and HIV prevalence in Malaysia: a cross-sectional study.","authors":"Hakimi Kassim, Kamarul Ismail, Md Enamul Huq, Hein Htet Aung, Ethan Trey Cardwell, Fabian Yuh Shiong Kong","doi":"10.1186/s13104-026-07920-5","DOIUrl":"10.1186/s13104-026-07920-5","url":null,"abstract":"Objective: Men who have sex with men in Malaysia face a high HIV burden, but demographic, behavioral, and digital health data remain limited by social constraints. This nationwide, cross-sectional ecological study utilized a grid-based geographic sampling method to systematically collect publicly accessible Grindr profiles across all Malaysian states and federal territories. Population-normalized Grind user rates, profile characteristics, and regional people living with HIV rates were analyzed using Spearman's rank-order correlation to evaluate whether geosocial networking platform data offer insights into regional HIV epidemiology.Results: A significant positive correlation was observed between population-normalized Grindr user rates and PLHIV rates (rs = 0.5821, P = 0.0252). While unadjusted analyses showed nominal positive correlations between regional PLHIV rates and specific profile characteristics, none maintained significance following false discovery rate correction (Q = 5%). This lack of post-correction significance is driven by the limited statistical power of the regional sample size (n = 16) and high percentages of profile non-disclosure. Thus, while grid-based sampling systematically assesses digital user density, individual profile characteristics cannot reliably indicate regional HIV prevalence when data omissions are widespread.","PeriodicalId":9234,"journal":{"name":"BMC Research Notes","volume":" ","pages":""},"PeriodicalIF":1.7,"publicationDate":"2026-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13523420/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148276174","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0