噬菌体tec -噬菌体tg和BC-VP对致病性大肠杆菌生物膜的抗菌活性研究。

IF 1.7 Q2 MULTIDISCIPLINARY SCIENCES

BMC Research Notes Pub Date : 2025-08-14 DOI:10.1186/s13104-025-07432-8

King Archell, Javier Stuart, Diana Elizabeth Waturangi

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引用次数: 0

摘要

目的：测定淡水湖泊中分离的噬菌体ETEC-phage-TG和BC-VP对聚苯乙烯和不锈钢表面肠出血性大肠杆菌和肠致病性大肠杆菌生物膜的抗菌活性。结果：从先前的研究中分离出的噬菌体具有降解生物膜的潜力。噬菌体ETEC-Phage-TG对肠出血性大肠杆菌的滴度为4.50 × 105 PFU/mL，最小感染抑制倍数为0.01（10- 2）。噬菌体BC-VP对EPEC的效价为8.0877 × 104 PFU/mL，最小感染抑制倍数为0.001（10- 3）。噬菌体ec -phage- tg对聚苯乙烯表面生物膜的抑制和破坏率分别为25.43%和11.41%。噬菌体ec -phage- tg对不锈钢表面生物膜形成的破坏率分别为37.87%和39.21% （24 h和48 h）。BC-VP噬菌体对聚苯乙烯表面生物膜的抑制和破坏率分别为54.57%和26.36%，对不锈钢表面生物膜形成（24 h和48 h）的破坏率分别为33.21%和43.94%。

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Investigation of antibiofilm activity of bacteriophage ETEC-phage-TG and BC-VP against pathogenic Escherichia coli biofilms.

Objectives: The aim of this study was to determine the antibiofilm activity of bacteriophages, ETEC-phage-TG and BC-VP, isolated from freshwater lakes against biofilm of Enterohemorrhagic Escherichia coli and Enteropathogenic Escherichia coli on polystyrene and stainless-steel surfaces.

Results: Bacteriophages isolated from previous research have the potential to degrade biofilm. Bacteriophage ETEC-Phage-TG have titer 4.50 × 10⁵ PFU/mL towards EHEC and a Minimum Inhibitory Multiplicity of Infection of 0.01 (10^- 2). Bacteriophage BC-VP had titer 8.0877 × 10⁴ PFU/mL towards EPEC and a Minimum Inhibitory Multiplicity of Infection of 0.001 (10^- 3). The bacteriophage ETEC-phage-TG was able to inhibit and destruct biofilm formation on polystyrene surface at 25.43% and 11.41% respectively. Bacteriophage ETEC-phage-TG was able to destruct biofilm formation (24 h and 48 h) on stainless steel surface at 37.87% and 39.21% respectively. Bacteriophage BC-VP was able to inhibit and destruct biofilm on polystyrene surface at 54.57% and 26.36% respectively, it was also able to destruct biofilm formation (24 h and 48 h) on stainless steel surface 33.21% and 43.94% respectively.

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来源期刊

BMC Research Notes Biochemistry, Genetics and Molecular Biology-Biochemistry, Genetics and Molecular Biology (all)

CiteScore

3.60

自引率

0.00%

发文量

363

审稿时长

15 weeks

期刊介绍： BMC Research Notes publishes scientifically valid research outputs that cannot be considered as full research or methodology articles. We support the research community across all scientific and clinical disciplines by providing an open access forum for sharing data and useful information; this includes, but is not limited to, updates to previous work, additions to established methods, short publications, null results, research proposals and data management plans.