BMC Musculoskeletal Disorders最新文献

{"title":"Predictive factors of distal pedicle screw loosening followed posterior corrective surgery for degenerative lumbar scoliosis.","authors":"Hongru Yuan, Yilei Liu, Chenjie Dai, Puxin Yang, Yachong Huo, Di Zhang, Hui Wang","doi":"10.1186/s12891-025-08519-w","DOIUrl":"10.1186/s12891-025-08519-w","url":null,"abstract":"<p><strong>Introduction: </strong>To explore incidence and predictive factors for distal pedicle screw loosening (DPSL) followed posterior corrective surgery for degenerative lumbar scoliosis (DLS).</p><p><strong>Methods: </strong>The diagnostic criteria for DPSL developed by X-ray including radiolucent area around screw and \"double halo\" sign. According to occurrence of DPSL at two-year follow-up, 153 patients were divided into two groups: study group (screw loosening) and control group (without screw loosening). To investigate predictive factors for DPSL, three categorized factors including general data, surgical data and radiological data were analyzed statistically.</p><p><strong>Results: </strong>DPSL was detected in 72 patients at two-year follow up (study group). Hounsfield unit (HU) value was lower in study group than that in control group. Fusion level was longer in study group than that in control group. Lower instrumented vertebrae on L5 was less in study group than that in control group. Posterolateral fusion was less in study group than that in control group. Preoperative Cobb angle, postoperative Cobb angle, Cobb angle correction, preoperative lumbosacral coronal angle (LSCA), LSCA correction, preoperative thoracolumbar junction (TL), postoperative TL were larger in study group than those in control group. Logistic regression analysis revealed that low Bone mineral density (BMD) (< 169 HU), posterolateral fusion, Cobb angle correction (> 16 degrees), LSCA correction (> 9 degrees) were independently associated with DPSL.</p><p><strong>Conclusions: </strong>The incidence of DPSL following posterior decompression and instrumented fusion for DLS is 47.1%. Low BMD, large correction of both main curve and fractional curve are predictive factors for DPSL, posterolateral fusion is a protective factor.</p>","PeriodicalId":9189,"journal":{"name":"BMC Musculoskeletal Disorders","volume":"26 1","pages":"283"},"PeriodicalIF":2.2,"publicationDate":"2025-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11929243/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143691116","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Machine learning-based radiomics using MRI to differentiate early-stage Duchenne and Becker muscular dystrophy in children.","authors":"Taiya Chen, Haoran Zhu, Yingyi Hu, Yang Huang, Wengan He, Yizhen Luo, Zeqi Wu, Diangang Fang, Longwei Sun, Hongwu Zeng, Zhiyong Li","doi":"10.1186/s12891-025-08538-7","DOIUrl":"10.1186/s12891-025-08538-7","url":null,"abstract":"<p><strong>Objectives: </strong>Duchenne muscular dystrophy (DMD) and Becker muscular dystrophy (BMD) present similar symptoms in the early stage, complicating their differentiation. This study aims to develop a classification model using radiomic features from MRI T2-weighted Dixon sequences to increase the accuracy of distinguishing DMD and BMD in the early disease stage.</p><p><strong>Methods: </strong>We retrospectively analysed MRI data from 62 patients aged 36-60 months with muscular dystrophy, including 41 with DMD and 21 with BMD. Radiomic features were extracted from in-phase, opposed-phase, water, fat, and postprocessed fat fraction images. We employed a deep learning segmentation method to segment regions of interest automatically. Feature selection included the Mann‒Whitney U test for identifying significant features, Pearson correlation analysis to remove collinear features, and the LASSO regression method to select features with nonzero coefficients. These selected features were then used in various machine learning algorithms to construct the classification model, and their diagnostic performance was compared.</p><p><strong>Results: </strong>Our proposed radiomic and machine learning methods effectively distinguished early DMD and BMD. The machine learning models significantly outperformed the radiologists in terms of accuracy (81.2-90.6% compared with 69.4%), specificity (71.0-86.0% compared with 19.0%), and F1 score (85.2-92.6% compared with 80.5%), while maintaining relatively high sensitivity (85.6-95.0% compared with 95.1%).</p><p><strong>Conclusion: </strong>Radiomics based on Dixon sequences combined with machine learning methods can effectively distinguish between DMD and BMD in the early stages, providing a new and effective tool for the early diagnosis of these muscular dystrophies.</p><p><strong>Clinical trial number: </strong>Not applicable.</p>","PeriodicalId":9189,"journal":{"name":"BMC Musculoskeletal Disorders","volume":"26 1","pages":"287"},"PeriodicalIF":2.2,"publicationDate":"2025-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11929326/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143691114","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Femoral inherent torsion is more accurate than femoral anteversion angle in evaluating femoral torsion to determine whether combine derotational distal femoral osteotomy or not.","authors":"Zhengyi Ni, Kehan Li, Xiaobo Chen, Yitong Hu, Jingting Zhang, Fei Wang","doi":"10.1186/s12891-025-08522-1","DOIUrl":"10.1186/s12891-025-08522-1","url":null,"abstract":"<p><strong>Background: </strong>Previous view is that femoral anteversion angle (FAA) is equivalent to femoral torsion (FT) and as an indication for derotational distal femoral osteotomy (DDFO) combined with medial patellofemoral ligament reconstruction (MPFLR), but posterior femoral condylar deformity affects FAA. Therefore, FAA is not accurate in assessing FT. Whether the femoral inherent torsion (FIT), which avoids the influence of the posterior condyle, can better reflect FT during surgery remains unknown. Meanwhile, the impact of the posterior femoral condyle on surgical outcomes remains unclear.</p><p><strong>Methods: </strong>Twenty-five patellar dislocation (PD) patients from 2017 to 2021 were conducted. All patients underwent both preoperative and postoperative computed tomography scans. Categorized by posterior condylar angle (PCA), they were divided into Group A (PCA ≤ 6.4°) and Group B (PCA > 6.4°). Radiographic measurements included FAA, femoral inherent torsion (FIT), patellar tilt angle, congruence angle and tibial tubercle-trochlear groove distance. For clinical outcomes, the Kujula score, Lysholm score, IKDC score to reflect the knee function. The Tegner activity score was used to assess the activity level. The VAS score was used to assess the pain control.</p><p><strong>Results: </strong>In both groups, the postoperative radiographic outcomes demonstrated a statistically significant improvement. Preoperatively, the FAA was similar in the two groups, but the FIT was greater in the Group A (21.7° ± 1.2° vs 18.4° ± 1 .3°, P < 0.001). However, there was no statistically significant difference between them in the postoperative period (7.4° ± 1.5° vs 7.1° ± 1.8°). In terms of clinical outcomes, both groups demonstrated a significant improvement in the postoperative period. However, the scores of the Group A significantly better (Kujula: 85.7 ± 5.0 vs 79.6 ± 4.8, P = 0.005; Lysholm: 86.8 ± 5.3 vs 80.2 ± 5.7, P = 0.006; IKDC: 86.1 ± 8.8 vs 75.5 ± 7.6, P = 0.004).</p><p><strong>Conclusion: </strong>FIT may be a more reliable indicator than FAA for evaluating FT in PD to determine whether combine DDFO or not, especially in the presence of posterior femoral condylar deformity. Posterior femoral condylar deformity appeared to result in a pseudo-increase in FAA. Simultaneous evaluation of FIT and FAA to identify true posterior condylar deformity offers the potential to prevent enlargement of DDFO and enable precision treatment.</p>","PeriodicalId":9189,"journal":{"name":"BMC Musculoskeletal Disorders","volume":"26 1","pages":"284"},"PeriodicalIF":2.2,"publicationDate":"2025-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11929340/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143691100","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Recurrent diffuse tenosynovial giant cell tumor of the posterior knee: a rare presentation with synovial herniation (Baker's cyst) in a young patient.","authors":"Khalid AlHarbi, Sultan AlHussain","doi":"10.1186/s12891-025-08511-4","DOIUrl":"10.1186/s12891-025-08511-4","url":null,"abstract":"<p><strong>Introduction: </strong>Tenosynovial giant cell tumor (TGCT), previously referred to as pigmented villonodular synovitis (PVNS), is a rare, locally aggressive proliferative disorder of the synovium. It typically affects large joints, most commonly the knee. Posterior compartment involvement in the knee is less common compared to anterior or suprapatellar involvement, and it presents unique diagnostic and therapeutic challenges due to the complex anatomy and proximity to neurovascular structures. While popliteal (Baker's) cysts may coexist with intra-articular TGCT, they are considered synovial herniation cysts rather than true extra-articular involvement, which can be associated with diagnostic and therapeutic challenges.</p><p><strong>Case presentation: </strong>This case report describes a 24-year-old male with recurrent diffuse TGCT of the knee, predominantly involving the posterior compartment, along with a large Baker's cyst and synovial thickening adjacent to the medial femoral condyle. Magnetic resonance imaging (MRI) revealed extensive synovial proliferation with characteristic low T2 signal intensity, confirming the diagnosis and guiding surgical planning. The patient underwent arthroscopic-assisted synovectomy to address both the suprapatellar and posterior compartments while preserving critical neurovascular structures. Postoperatively, the patient had improved knee function and was referred for adjuvant therapy to minimize recurrence risk.</p><p><strong>Conclusion: </strong>This case highlights the importance of accurate imaging, meticulous surgical planning, and a multidisciplinary approach in managing rare presentations of TGCT. Long-term follow-up and consideration of adjuvant therapies such as radiotherapy or systemic therapies, including Pexidartinib, remain essential to minimize recurrence and optimize outcomes.</p>","PeriodicalId":9189,"journal":{"name":"BMC Musculoskeletal Disorders","volume":"26 1","pages":"281"},"PeriodicalIF":2.2,"publicationDate":"2025-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11929234/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143691118","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bioinformatics and machine learning approaches to explore key biomarkers in muscle aging linked to adipogenesis.","authors":"Yumin Zhang, Li Qin, Juan Liu","doi":"10.1186/s12891-025-08528-9","DOIUrl":"10.1186/s12891-025-08528-9","url":null,"abstract":"<p><p>Adipogenesis is intricately linked to the onset and progression of muscle aging; however, the relevant biomarkers remain unclear. This study sought to identify key genes associated with adipogenesis in the context of muscle aging. Firstly, gene expression profiles from biopsies of the vastus lateralis muscle in both young and elderly population were retrieved from the GEO database. After intersecting with the results of differential gene analysis, weighted gene co-expression network analysis, and sets of adipogenesis-related genes, 29 adipogenesis-related differential expressed genes (ARDEGs) were selected. Connectivity Map (cMAP) analysis identified tamsulosin, fraxidin, and alaproclate as key target compounds. In further, using three machine learning algorithms and the friends analysis, four hub ARDEGs, ESRRA, RXRG, GADD45A, and CEBPB were identified and verified in vivo aged mice muscles. Immune infiltration analysis showed a strong link between several immune cells and hub ARDEGs. In all, these findings suggested that ESRRA, RXRG, GADD45A, and CEBPB could serve as adipogenesis related biomarkers in muscle aging.</p>","PeriodicalId":9189,"journal":{"name":"BMC Musculoskeletal Disorders","volume":"26 1","pages":"285"},"PeriodicalIF":2.2,"publicationDate":"2025-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11929359/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143691099","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Arthroscopic Bankart repair using trans-glenoid double-loaded grand knots versus double-loaded suture anchors; is there a difference? a randomized controlled study.","authors":"Amr Samir Rashwan, Al-Qassem Amin, Sherif Hamdy Zawam, Alaa Mohy-Eldin Soliman, Mahmoud El-Desouky","doi":"10.1186/s12891-025-08477-3","DOIUrl":"10.1186/s12891-025-08477-3","url":null,"abstract":"<p><strong>Background: </strong>Anatomical repair of Bankart lesions and restoring the tension of the antero-inferior capsulo-labral complex is the optimum method of surgical treatment with a variety of fixation methods including suture anchors and trans-glenoid sutures. Grand knot technique is a modification of the trans-glenoid sutures technique that can be an alternative to double-loaded suture anchors with a lower cost. We aimed to compare the outcomes and complications of both techniques.</p><p><strong>Methods: </strong>This is a randomized controlled study that was conducted on 200 patients with recurrent anterior glenohumeral dislocation, of whom 170 patients completed at least a three-year follow-up period. Arthroscopic Bankart repair using two double-loaded knotted suture anchors was performed in 78 cases (Group A) while repair was done using two trans-glenoid grand knots in other 92 cases (Group B). Patients were evaluated in terms of range of motion, functional scores (Constant, Rowe, and ASES), and complication rate.</p><p><strong>Results: </strong>The mean operative time was significantly longer in Group B (87.7 ± 24) minutes compared to Group A (61.2 ± 28.1) minutes (P = 0.002). No statistically significant difference was found between both groups regarding postoperative external rotation range of adducted arm, functional scores, and rate of recurrence. Only forward flexion and external rotation of abducted arm were significantly better in Group A (P = 0.005 and < 0.001 respectively).</p><p><strong>Conclusion: </strong>Trans-glenoid double-loaded grand knot technique is an alternative surgical option for the treatment of Bankart lesions with comparable results to double-loaded anchors regarding the functional outcomes and failure rates.</p><p><strong>Clinical trial registration (retrospectively registered): </strong>Registration number: NCT06394609 28-4-2024.</p>","PeriodicalId":9189,"journal":{"name":"BMC Musculoskeletal Disorders","volume":"26 1","pages":"280"},"PeriodicalIF":2.2,"publicationDate":"2025-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11927316/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143668935","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparison of the clinical efficacy of patellar lateral retraction and patellar eversion in total knee arthroplasty: a systematic review and meta-analysis.","authors":"Mingjie Dong, Xiaoyu Sun, Hao Fan, Weiping Ren, Yushan Wang, Yingjie Gao, Pengfei Shao, Yu Gao, Qiang Jiao, Yi Feng","doi":"10.1186/s12891-025-08532-z","DOIUrl":"10.1186/s12891-025-08532-z","url":null,"abstract":"<p><strong>Background: </strong>According to the mobilization technique of the intraoperative patella, total knee arthroplasty (TKA) can be categorized into patellar eversion (PE) and patellar lateral retraction (PLR). Comparisons between the two procedures are inconclusive; therefore, the study purpose was to assess the postoperative clinical efficacy to identify the most suitable procedure.</p><p><strong>Methods: </strong>Electronic databases were searched, including Web of Science, ScienceDirect, PubMed, Embase, OVID, the Cochrane Library, CINAHL, CNKI, and WANFANG, to identify clinical trials of PLR versus PE from inception to May 2023. The statistical software Stata 15.0 and Review Manager 5.4 were applied to the data analysis.</p><p><strong>Results: </strong>Fifteen studies evaluating a total of 1349 patients and 1409 knees were ultimately included. Statistically significant differences emerged between the PLR and PE groups with respect to blood loss (P = 0.02), incision length (P < 0.001), operation time (P = 0.01), straight leg raise (P < 0.001), knee range of motion (ROM; P < 0.05), the Knee Society Score (KSS) functional score (P = 0.0003), the visual analogue scale (VAS) score (1 and 3 months, both P < 0.05), and operative complications (P = 0.02). Furthermore, the PLR and PE groups had similar clinical efficacy in terms of quadriceps strength, VAS score (1 week and 1 year), Hospital for Special Surgery score, KSS pain score, Insall-Salvati ratio, and the occurrence of patella baja (all P ≥ 0.05).</p><p><strong>Conclusions: </strong>The PLR procedure is superior to PE in terms of blood loss, incision length, straight leg raise, knee ROM, VAS score (1 and 3 months), KSS functional score, and operative complications, although PE could decrease the operation time. PLR could achieve better postoperative clinical outcomes than could PE. Therefore, it is recommended that experienced surgeons prioritize PLR in TKA.</p><p><strong>Registration: </strong>This study was registered in the PROSPERO international registry (Registration ID: CRD42023440722).</p>","PeriodicalId":9189,"journal":{"name":"BMC Musculoskeletal Disorders","volume":"26 1","pages":"279"},"PeriodicalIF":2.2,"publicationDate":"2025-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11924621/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143662595","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The prevalence and surgical outcome of late diagnosed hip dysplasia in children with Prader-Willi syndrome: a retrospective study.","authors":"Yueqiang Mo, Chunxing Wu, Peng Huang, Dahui Wang, Yanhui Jing, Bo Ning","doi":"10.1186/s12891-025-08470-w","DOIUrl":"10.1186/s12891-025-08470-w","url":null,"abstract":"<p><strong>Background: </strong>Prader-Willi syndrome (PWS) is a rare disease. Hip dysplasia is an orthopedic-related disease of PWS. Limited literature exists on the prevalence, diagnosis, and surgical management of late diagnosed hip dysplasia in PWS. This study assessed the prevalence of hip dysplasia in children with PWS and evaluated the outcomes following surgical intervention of late diagnosed hip dysplasia.</p><p><strong>Methods: </strong>A retrospective analysis was conducted on patients diagnosed with PWS at our institution from January 1, 2017 to December 31, 2021. Patient demographics were collected, the acetabular index (AI) and the central edge angle (CEA) were measured. A single fellowship-trained pediatric orthopedic surgeon determined the presence of hip dysplasia based on radiographic measurements.</p><p><strong>Results: </strong>The prevalence of hip dysplasia with PWS is 33.3%. There was no significant association between prevalence and sex or genetic subtype. The mean age at the time of diagnosis was 34.4 months (6 months to 109 months). Five patients (2 right side, 3 bilateral) underwent surgical intervention at an average age of 82.4 months. The acetabular index decreased from 42.8 ± 5.9 degrees preoperatively to 21.7 ± 7.7 degrees postoperatively. No serious postoperative complications were reported during the follow-up.</p><p><strong>Conclusions: </strong>The present study demonstrated a higher prevalence of hip dysplasia in patients with PWS than in the general population. The prevalence does not significantly differ across sexes or genetic subtypes. While preliminary findings suggest outcomes may be comparable to those of HD without PWS, further studies with larger cohorts are required to validate these observations.</p>","PeriodicalId":9189,"journal":{"name":"BMC Musculoskeletal Disorders","volume":"26 1","pages":"278"},"PeriodicalIF":2.2,"publicationDate":"2025-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11924822/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143662613","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Muscle loading and endochondral ossification are involved in the regeneration of a fibrocartilaginous enthesis during tendon to bone healing in rabbits.","authors":"Xiaoke Shang, Can Chen, Jiefu Zhou, Yang Yang, Jin Qu","doi":"10.1186/s12891-025-08508-z","DOIUrl":"10.1186/s12891-025-08508-z","url":null,"abstract":"<p><strong>Purpose: </strong>The purposes of the present study are to investigate the effects of reduced muscle loading by prolonged immobilization on the regeneration of fibrocartilaginous enthesis through endochondral ossification in rabbits.</p><p><strong>Methods: </strong>Forty-eight rabbits underwent standard partial patellectomy were randomly divided into the control group and the prolonged immobilization (PIM) group. The immobilized cast was only maintained for the first 4 weeks in the control group, while for the first 12 weeks or until euthanization in the PIM group. The Patella-patella tendon complexes were harvested for Micro-CT and histology at week 6, 12 and 18.</p><p><strong>Results: </strong>There was significantly lower bone volume in the PIM group than the control group at week 12, but not at week 6 and 18. At week 6, new bone was formed at the osteotomy site of the residual patella through endochondral ossification. At week 12, the chondrocytes in the tendon to bone interface were ordered and arranged in longitudinal rows separated by collagen fibres in the control group. While there were no visible fibers running continuously from tendon into bone in the PIM group. At week 18, a nearly normal fibrocartilaginous enthesis were regenerated in the control group. A similar fibrocartilaginous enthesis were also formed at the tendon to bone interface in the PIM group, but the four zones were not as distinct as that in the control group.</p><p><strong>Conclusion: </strong>Muscle loading and endochondral ossification are involved in the regeneration of a fibrocartilaginous enthesis during tendon to bone healing in this partial patellectomy model.</p>","PeriodicalId":9189,"journal":{"name":"BMC Musculoskeletal Disorders","volume":"26 1","pages":"277"},"PeriodicalIF":2.2,"publicationDate":"2025-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11921697/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143662611","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hepcidin knockout exacerbates hindlimb unloading-induced bone loss in mice through inhibiting osteoblastic differentiation.","authors":"Xin Chen, Jianping Wang, Chenxiao Zhen, Gejing Zhang, Zhouqi Yang, Youjia Xu, Peng Shang","doi":"10.1186/s12891-025-08515-0","DOIUrl":"10.1186/s12891-025-08515-0","url":null,"abstract":"<p><strong>Background: </strong>An oligopeptide hepcidin is encoded by the human HAMP gene (Hamp in mice). Its deficiency can result in iron overload, while excess may lead to iron deficiency. Hepcidin knockout mice exhibited iron accumulation in multiple tissues, accompanied by degeneration of bone microarchitecture and reduced biomechanical properties. Astronauts who are exposed to weightlessness during prolonged spaceflight experience bone loss. After space missions, an interrelation exists between iron stores and bone mineral density (BMD). Bone loss in mice due to unloading is linked to iron excess and involves hepcidin. The potential role of hepcidin in unloading-induced bone loss remains unclear.</p><p><strong>Methods: </strong>Our study conducted relevant experiments using hepcidin knockout mice and their primary osteoblasts as the research subjects. We used the hindlimb unloading (HLU) model and the random positioning machine (RPM) system to simulate weightlessness in vivo and in vitro.</p><p><strong>Results: </strong>HLU mice exhibited reduced hepcidin levels in the serum and liver. Hepcidin knockout further diminished BMD and bone mineral content (BMC) in the femurs of HLU mice. Similarly, the bone volume fraction (BV/TV) and connectivity density (Conn.Dn) followed this downward trend, whereas trabecular separation (Tb.Sp) showed an inverse pattern. Moreover, hepcidin knockout decreased the ultimate load and elastic modulus in the tibias of HLU mice. Hepcidin knockout decreased PINP levels in the serum, a commonly used marker for bone formation, alongside elevated iron levels in the serum, liver, and bone of HLU mice. We also found higher serum MDA and SOD levels in these mice. In vitro, experimental data indicated that hepcidin knockout suppresses the osteoblastic differentiation capacity under RPM conditions. Additionally, this condition upregulates SOST protein levels and downregulates LRP6 and β-catenin protein levels in osteoblasts.</p><p><strong>Conclusion: </strong>Hepcidin knockout exacerbates bone loss in HLU mice, most likely due to reduced osteoblastic activity.</p>","PeriodicalId":9189,"journal":{"name":"BMC Musculoskeletal Disorders","volume":"26 1","pages":"276"},"PeriodicalIF":2.2,"publicationDate":"2025-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11917043/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143655819","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}