BMC Molecular and Cell Biology最新文献_第3页

Genetic suppressor of autophagy defects in huntingtin null cells identified using a mutagenesis screen in Dictyostelium discoideum. 利用诱变筛选在盘状盘齿钢中鉴定亨廷顿蛋白缺失细胞中自噬缺陷的遗传抑制因子。

IF 2.7 3区生物学

BMC Molecular and Cell Biology Pub Date : 2026-02-02 DOI: 10.1186/s12860-026-00570-2

Lauren Cuoco, Shelbi E Gill, Luc Francois, Sarah Souders, Caroline DeMasi, Jeffrey R Moore, Frédéric J J Chain

{"title":"Genetic suppressor of autophagy defects in huntingtin null cells identified using a mutagenesis screen in Dictyostelium discoideum.","authors":"Lauren Cuoco, Shelbi E Gill, Luc Francois, Sarah Souders, Caroline DeMasi, Jeffrey R Moore, Frédéric J J Chain","doi":"10.1186/s12860-026-00570-2","DOIUrl":"10.1186/s12860-026-00570-2","url":null,"abstract":"Background: Huntingtin (HTT) is an important gene for cellular processes such as autophagy, and its loss leads to neurodegenerative disease phenotypes. In Dictyostelium discoideum, HTT-null (htt-) cells exhibit impaired basal autophagy and fail to develop in the presence of ammonium chloride.Results: Here we conducted a mutagenesis screen on htt- cells to identify potential genetic suppressors of the htt- phenotype. A mutant strain was isolated that counteracts many of the hallmark defects engendered with htt loss. This mutant, htt-;supX, rescues the growth, cargo degradation defects, developmental timing, and autophagic flux of htt- cells under ammonium chloride stress, representing a partial rescue of the htt- phenotype. Whole-genome sequencing revealed four mutations in the mutant strain affecting genes involved in vesicle trafficking, signal transduction, metal ion regulation, and fatty acid elongation. Transcriptome sequencing further identified 208 differentially expressed genes in the mutant strain, including genes whose expression was returned to wild-type levels, suggesting a potential mechanism by which htt-;supX mediates phenotypic recovery. Among these, five genes have known autophagy-related functions and may be implicated in pathways such as Rab GTPase regulation and SNARE-mediated vesicle fusion.Conclusions: Our study highlights the ability of second-site mutations to restore autophagic function in the absence of HTT and identifies candidate genes and pathways for further investigation into Huntington's Disease models and autophagy modulation.","PeriodicalId":9099,"journal":{"name":"BMC Molecular and Cell Biology","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2026-02-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12954983/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146104115","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Wnt/β-catenin pathway maintains homeostasis of amniocytes in Down syndrome. Wnt/β-catenin通路维持唐氏综合征羊膜细胞的稳态。

IF 2.7 3区生物学

BMC Molecular and Cell Biology Pub Date : 2026-01-31 DOI: 10.1186/s12860-026-00569-9

Xiaoying Chen, Miaochun Lin, Shan Chen, Zhengsen Wang, Zhaohui Li, Juan Zuo

引用次数: 0

Isolation and characterization of mesenchymal stem cells from nasal polyps and olfactory mucosa: a comparative analysis of proliferation and multi-lineage differentiation capacity. 鼻息肉和嗅觉粘膜间充质干细胞的分离和鉴定：增殖和多系分化能力的比较分析。

IF 2.7 3区生物学

BMC Molecular and Cell Biology Pub Date : 2026-01-30 DOI: 10.1186/s12860-026-00564-0

Negin Khosravi, Zahra Pourmohammadi-Bejarpasi, Mehryar Habibi Roudkenar, Samin Abed, Ehsan Kazemnezhad Leyli, Shadman Nemati

引用次数: 0

Effects of irisin on inflammatory and apoptotic markers in the Caco-2 colon cancer cell line. 鸢尾素对Caco-2结肠癌细胞炎症和凋亡标志物的影响。

IF 2.7 3区生物学

BMC Molecular and Cell Biology Pub Date : 2026-01-22 DOI: 10.1186/s12860-026-00568-w

Elif Zeynep Ozturk, Ebubekir Bakan, Nurcan Kilic Baygutalp, Zafer Bayraktutan

引用次数: 0

Dysregulation of caspase-8 and caspase-9 in T-lymphocyte apoptosis: implications for pathogenesis, diagnosis, and therapeutic targeting in psoriatic arthritis. t淋巴细胞凋亡中caspase-8和caspase-9的失调：银屑病关节炎的发病机制、诊断和治疗靶向的意义

IF 2.7 3区生物学

BMC Molecular and Cell Biology Pub Date : 2026-01-18 DOI: 10.1186/s12860-026-00565-z

Hadeel A Al-Rawaf, Sami A Gabr, Amir Iqbal, Ahmad H Alghadir

引用次数: 0

Intracellular Ca²⁺ is not essential for SHH signaling but is promoted by Shh ligand in embryonic fibroblasts. 在胚胎成纤维细胞中，细胞内Ca2+对SHH信号传导不是必需的，但可由SHH配体促进。

IF 2.7 3区生物学

BMC Molecular and Cell Biology Pub Date : 2026-01-18 DOI: 10.1186/s12860-026-00567-x

Xuanming Shi, Zhaomin Wang, Shupeng Li, Yiming Pan, Bing Shen, Shuzhen Liu

引用次数: 0

Mitochondrial HMGCS1 mediates cisplatin resistance in cervical cancer through regulation of mitochondrial transcription. 线粒体HMGCS1通过调控线粒体转录介导宫颈癌顺铂耐药。

IF 2.7 3区生物学

BMC Molecular and Cell Biology Pub Date : 2026-01-16 DOI: 10.1186/s12860-026-00566-y

Wenxuan Yang, Shumin Liu, Dandan Shang, Ping Liu, Hongtang Shi, Hongtao Zhang, Chao Zhou

引用次数: 0

The nascent RNA labelling compound 5-ethynyl uridine (EU) integrates into DNA in some animals. 新生的RNA标记化合物5-乙基尿苷（EU）在一些动物中整合到DNA中。

IF 2.7 3区生物学

BMC Molecular and Cell Biology Pub Date : 2025-12-26 DOI: 10.1186/s12860-025-00560-w

Malin A Kjosavik, Katherine L P Downham, Ruth Styfhals, Leonie Adelmann, Marios Chatzigeorgiou, Florian Raible, Pawel Burkhardt, Fergal O'Farrell, Patrick R H Steinmetz, Kathrin Garschall

引用次数: 0

IF 2.7 3区生物学

BMC Molecular and Cell Biology Pub Date : 2025-12-25 DOI: 10.1186/s12860-025-00562-8

Nicholas M Kiger, Nicholas W Mason, Md Ikram Rafid, Jeffrey A Hadwiger

引用次数: 0

Molecular architecture of mesoderm cells across early to middle stage of human embryo development at single-cell resolution. 单细胞分辨率下人类胚胎发育早期至中期中胚层细胞的分子结构。

IF 2.7 3区生物学

BMC Molecular and Cell Biology Pub Date : 2025-12-25 DOI: 10.1186/s12860-025-00561-9

Wei Zhang, Haiyan Yu, Zhibin Zhang, Dandan Li, Yane Yang, Wei Shi, Wenting Li, Yan Jiang, Wenlong Hu, Zhipeng Zeng, Xinqiong Liu, Zhanye Zheng, Minglin Ou, Donge Tang, Yong Dai

{"title":"Molecular architecture of mesoderm cells across early to middle stage of human embryo development at single-cell resolution.","authors":"Wei Zhang, Haiyan Yu, Zhibin Zhang, Dandan Li, Yane Yang, Wei Shi, Wenting Li, Yan Jiang, Wenlong Hu, Zhipeng Zeng, Xinqiong Liu, Zhanye Zheng, Minglin Ou, Donge Tang, Yong Dai","doi":"10.1186/s12860-025-00561-9","DOIUrl":"10.1186/s12860-025-00561-9","url":null,"abstract":"Background: The differentiation of mesodermal cells (MCs) in the early stage of embryonic development contributes to the organogenesis of several core organs. However, the single-cell molecular architecture of MCs and the key molecular events during the differentiation remain unclear.Methods: We performed single-cell RNA sequencing (RNA sequencing) and single-cell assay for transposase-accessible chromatin (ATAC-Seq) to analyze the developmental features of MCs to heart, kidney, spleen, liver, and brain in human embryos at gestational ages 7-17 weeks.Results: We found that EGR1 might be relevant to the differentiation of heterogeneous MC sub-clusters. Meanwhile, RPL10P9+PTMAP5+ MCs had the closest expression profiling with endocardial cells. NDUFA4L2+A2M+ MCs presented the potentials to form endothelial cells (ECs) and hematopoietic stem cells, and MEF2C might be involved in this process.Conclusions: These findings provide insights into the molecular architecture and lineage progression of MCs during early human embryonic organogenesis, offering a valuable reference for regenerative medicine and organ bioengineering.","PeriodicalId":9099,"journal":{"name":"BMC Molecular and Cell Biology","volume":" ","pages":"3"},"PeriodicalIF":2.7,"publicationDate":"2025-12-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12849250/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145833014","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0