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Induction of iPSC-derived Prg4-positive cells with characteristics of superficial zone chondrocytes and fibroblast-like synovial cells. 诱导ipsc衍生的具有浅表带软骨细胞和成纤维细胞样滑膜细胞特征的prg4阳性细胞。
IF 2.8 3区 生物学
BMC Molecular and Cell Biology Pub Date : 2022-07-23 DOI: 10.1186/s12860-022-00431-8
Takashi Satake, Shingo Komura, Hitomi Aoki, Akihiro Hirakawa, Yuuki Imai, Haruhiko Akiyama
{"title":"Induction of iPSC-derived Prg4-positive cells with characteristics of superficial zone chondrocytes and fibroblast-like synovial cells.","authors":"Takashi Satake,&nbsp;Shingo Komura,&nbsp;Hitomi Aoki,&nbsp;Akihiro Hirakawa,&nbsp;Yuuki Imai,&nbsp;Haruhiko Akiyama","doi":"10.1186/s12860-022-00431-8","DOIUrl":"https://doi.org/10.1186/s12860-022-00431-8","url":null,"abstract":"<p><strong>Background: </strong>Lubricin, a proteoglycan encoded by the PRG4 gene, is synthesised by superficial zone (SFZ) chondrocytes and synovial cells. It reduces friction between joints and allows smooth sliding of tendons. Although lubricin has been shown to be effective against osteoarthritis and synovitis in animals, its clinical application remains untested. In this study, we aimed to induce lubricin-expressing cells from pluripotent stem cells (iPSCs) and applied them locally via cell transplantation.</p><p><strong>Methods: </strong>To generate iPSCs, OCT3/4, SOX2, KLF4, and L-MYC were transduced into fibroblasts derived from Prg4-mRFP1 transgenic mice. We established a protocol for the differentiation of iPSC-derived Prg4-mRFP1-positive cells and characterised their mRNA expression profile. Finally, we injected Prg4-mRFP1-positive cells into the paratenon, surrounding the Achilles tendons and knee joints of severe combined immunodeficient mice and assessed lubricin expression.</p><p><strong>Result: </strong>Wnt3a, activin A, TGF-β1, and bFGF were applied to induce the differentiation of iPSC-derived Prg4-mRFP1-positive cells. Markers related to SFZ chondrocytes and fibroblast-like synovial cells (FLSs) were expressed during differentiation. RNA-sequencing indicated that iPSC-derived Prg4-mRFP1-positive cells manifested expression profiles typical of SFZ chondrocytes and FLSs. Transplanted iPSC-derived Prg4-mRFP1-positive cells survived around the Achilles tendons and in knee joints.</p><p><strong>Conclusions: </strong>The present study describes a protocol for the differentiation of iPSC-derived Prg4-positive cells with characteristics of SFZ chondrocytes and FLSs. Transplantation of lubricin-expressing cells offers promise as a therapy against arthritis and synovitis.</p>","PeriodicalId":9099,"journal":{"name":"BMC Molecular and Cell Biology","volume":null,"pages":null},"PeriodicalIF":2.8,"publicationDate":"2022-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9308249/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40617457","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
High D-glucose levels induce ACE2 expression via GLUT1 in human airway epithelial cell line Calu-3. 高d -葡萄糖水平诱导人气道上皮细胞系Calu-3通过GLUT1表达ACE2。
IF 2.8 3区 生物学
BMC Molecular and Cell Biology Pub Date : 2022-07-15 DOI: 10.1186/s12860-022-00427-4
Yoshitaka Wakabayashi, Shin Nakayama, Ai Yamamoto, Takatoshi Kitazawa
{"title":"High D-glucose levels induce ACE2 expression via GLUT1 in human airway epithelial cell line Calu-3.","authors":"Yoshitaka Wakabayashi,&nbsp;Shin Nakayama,&nbsp;Ai Yamamoto,&nbsp;Takatoshi Kitazawa","doi":"10.1186/s12860-022-00427-4","DOIUrl":"https://doi.org/10.1186/s12860-022-00427-4","url":null,"abstract":"<p><strong>Background: </strong>Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) enters the host cell by binding to angiotensin-converting enzyme 2 (ACE2) receptors. ACE2 is expressed on human airway epithelial cells. Increased ACE2 expression may be associated with potentially high risk of COVID-19. However, the factors responsible for the regulation of ACE2 expression in human airway epithelial cells are unknown. Furthermore, hyperglycemia is a risk factor for poor disease prognosis.</p><p><strong>Results: </strong>In this study, we investigated the effects of D-glucose on ACE2 mRNA and protein expressions in Calu-3 bronchial submucosal cells. The cells were cultured in minimal essential medium containing different D-glucose concentrations. After 48 and 72 h of high D-glucose (1000 mg/dL) treatment, ACE2 mRNA expressions were significantly increased. ACE2 protein expressions were significantly increased after 24 h of high D-glucose treatment. ACE2 mRNA expression was enhanced by a D-glucose concentration of 550 mg/dL or more after 72 h of treatment. In addition, we investigated the role of glucose transporters (GLUTs) in Calu-3 cells. ACE2 mRNA and protein expressions were suppressed by the GLUT1 inhibitor BAY-876 in high D-glucose-treated Calu-3 cells. GLUT-1 siRNA was also used and ACE2 mRNA expressions were suppressed in high D-glucose-treated Calu-3 cells with GLUT-1 knockdown.</p><p><strong>Conclusions: </strong>This is the first report indicating that high D-glucose levels induced ACE2 expression via GLUT1 in bronchial submucosal cells in vitro. As hyperglycemia can be treated appropriately, these findings could help reduce the risk of worsening of coronavirus disease 2019.</p>","PeriodicalId":9099,"journal":{"name":"BMC Molecular and Cell Biology","volume":null,"pages":null},"PeriodicalIF":2.8,"publicationDate":"2022-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9282902/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40506059","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
MicroRNA-145-5p modulates Krüppel-like factor 5 and inhibits cell proliferation, migration, and invasion in nasopharyngeal carcinoma. MicroRNA-145-5p调节kr<s:1> ppel样因子5并抑制鼻咽癌细胞增殖、迁移和侵袭。
IF 2.8 3区 生物学
BMC Molecular and Cell Biology Pub Date : 2022-07-14 DOI: 10.1186/s12860-022-00430-9
Chien-Han Yuan, Wei-Chi Hsu, A-Mei Huang, Ben-Chih Yuan, I-Hung Chen, Chia-An Hsu, Rong-Feng Chen, Yih-Min Chu, Hui-Hui Lin, Hung-Lung Ke
{"title":"MicroRNA-145-5p modulates Krüppel-like factor 5 and inhibits cell proliferation, migration, and invasion in nasopharyngeal carcinoma.","authors":"Chien-Han Yuan,&nbsp;Wei-Chi Hsu,&nbsp;A-Mei Huang,&nbsp;Ben-Chih Yuan,&nbsp;I-Hung Chen,&nbsp;Chia-An Hsu,&nbsp;Rong-Feng Chen,&nbsp;Yih-Min Chu,&nbsp;Hui-Hui Lin,&nbsp;Hung-Lung Ke","doi":"10.1186/s12860-022-00430-9","DOIUrl":"https://doi.org/10.1186/s12860-022-00430-9","url":null,"abstract":"<p><strong>Background: </strong>In several human cancers, Krüppel-like factor 5 (KLF5), a zinc finger transcription factor, can contribute to both tumor progression or suppression; however, the precise role of KLF5 in nasopharyngeal carcinoma (NPC) remains poorly understood. In this study, the association between KLF5 and microRNA-145-5p (miR-145-5p) in NPC cells was elucidated.</p><p><strong>Results: </strong>Our results showed that KLF5 expression was up-regulated in NPC group compared to normal group. We found that KLF5 exhibited an oncogenic role in NPC cells. The upregulation of miR-145-5p inhibited the proliferation, migration, and invasion of NPC cells. It was observed that miR-145-5p could down-regulate the mRNA and protein expression of KLF5 in NPC cell lines. Additionally, the activity of focal adhesion kinase (FAK), a migration marker, was regulated by miR-145-5p and KLF5 in NPC cells.</p><p><strong>Conclusions: </strong>The results of this study indicated that miR-145-5p could repress the proliferation, migration, and invasion of NPC cells via KLF5/FAK regulation, and could be a potential therapeutic target for patients with NPC.</p>","PeriodicalId":9099,"journal":{"name":"BMC Molecular and Cell Biology","volume":null,"pages":null},"PeriodicalIF":2.8,"publicationDate":"2022-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9284881/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40506063","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 3
Effects of the media conditioned by various macrophage subtypes derived from THP-1 cells on tunneling nanotube formation in pancreatic cancer cells. 由THP-1细胞衍生的各种巨噬细胞亚型调节的培养基对胰腺癌细胞隧道纳米管形成的影响
IF 2.8 3区 生物学
BMC Molecular and Cell Biology Pub Date : 2022-07-06 DOI: 10.1186/s12860-022-00428-3
Chia-Wei Lee, Chia-Chen Kuo, Chi-Jung Liang, Huei-Jyuan Pan, Chia-Ning Shen, Chau-Hwang Lee
{"title":"Effects of the media conditioned by various macrophage subtypes derived from THP-1 cells on tunneling nanotube formation in pancreatic cancer cells.","authors":"Chia-Wei Lee,&nbsp;Chia-Chen Kuo,&nbsp;Chi-Jung Liang,&nbsp;Huei-Jyuan Pan,&nbsp;Chia-Ning Shen,&nbsp;Chau-Hwang Lee","doi":"10.1186/s12860-022-00428-3","DOIUrl":"https://doi.org/10.1186/s12860-022-00428-3","url":null,"abstract":"<p><strong>Background: </strong>Tunneling nanotubes (TNTs) are special membrane structures for intercellular communications. Vital cargoes (such as mitochondria) could be delivered from healthy cells to rescue damaged ones through TNTs. The TNTs could be utilized for the purpose of systematic delivery of therapeutic agents between cells. However, there are insufficient studies on the controlled enhancement of TNT formations. The purpose of this study is to understand how macrophages influence the TNT formation in cancer cells.</p><p><strong>Results: </strong>Here we compared the capabilities of inducing TNTs in human pancreatic cancer cells (PANC-1) of the media conditioned by M0, M1 and M2 macrophages derived from THP-1 cells. The M0 and M1 macrophage conditioned media promoted TNT formation. Using a focused ion beam to cut through a TNT, we observed tunnel-like structures inside dense cytoskeletons with scanning electron microscopy. The TNT formation correlated with raised motility, invasion, and epithelial-mesenchymal transition in the PANC-1 cells. Mitochondria and lysosomes were also found to be transported in the TNTs.</p><p><strong>Conclusions: </strong>These results suggest that TNT formation could be one of the responses to the immune stress in pancreatic cancer cells caused by M0 and M1 macrophages. This finding is valuable for the development of macrophage-targeting cancer therapy.</p>","PeriodicalId":9099,"journal":{"name":"BMC Molecular and Cell Biology","volume":null,"pages":null},"PeriodicalIF":2.8,"publicationDate":"2022-07-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9258106/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40589065","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 3
Hik28-dependent and Hik28-independent ABC transporters were revealed by proteome-wide analysis of ΔHik28 under combined stress. 在联合胁迫下,通过蛋白质组分析ΔHik28揭示了依赖hik28和不依赖hik28的ABC转运蛋白。
IF 2.8 3区 生物学
BMC Molecular and Cell Biology Pub Date : 2022-07-06 DOI: 10.1186/s12860-022-00421-w
Pavinee Kurdrid, Rayakorn Yutthanasirikul, Sirilak Saree, Jittisak Senachak, Monpaveekorn Saelee, Apiradee Hongsthong
{"title":"Hik28-dependent and Hik28-independent ABC transporters were revealed by proteome-wide analysis of ΔHik28 under combined stress.","authors":"Pavinee Kurdrid,&nbsp;Rayakorn Yutthanasirikul,&nbsp;Sirilak Saree,&nbsp;Jittisak Senachak,&nbsp;Monpaveekorn Saelee,&nbsp;Apiradee Hongsthong","doi":"10.1186/s12860-022-00421-w","DOIUrl":"https://doi.org/10.1186/s12860-022-00421-w","url":null,"abstract":"<p><p>Synechocystis histidine kinase, Sll0474: Hik28, a signal protein in a two-component signal transduction system, plays a critical role in responding to a decrease in growth temperature and is also involved in nitrogen metabolism. In the present study, under combined stress from non-optimal growth temperature and nitrogen depletion, a comparative proteomic analysis of the wild type (WT) and a deletion mutant (MT) of Synechocystis histidine kinase, Sll0474: Hik28, in a two-component signal transduction system identified the specific groups of ABC transporters that were Hik28-dependent, e.g., the iron transporter, and Hik28-independent, e.g., the phosphate transporter. The iron transporter, AfuA, was found to be upregulated only in the WT strain grown under the combined stress of high temperature and nitrogen depletion. Whereas, the expression level of the phosphate transporter, PstS, was increased in both the WT and MT strains. Moreover, the location in the genome of the genes encoding Hik28 and ABC transporters in Synechocystis sp. PCC6803 were analyzed in parallel with the comparative proteomic data. The results suggested the regulation of the ABC transporters by the gene in a two-component system located in an adjacent location in the genome.</p>","PeriodicalId":9099,"journal":{"name":"BMC Molecular and Cell Biology","volume":null,"pages":null},"PeriodicalIF":2.8,"publicationDate":"2022-07-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9258054/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40476561","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Upregulation of CCT3 predicts poor prognosis and promotes cell proliferation via inhibition of ferroptosis and activation of AKT signaling in lung adenocarcinoma. 在肺腺癌中,CCT3表达上调可通过抑制铁下垂和激活AKT信号传导来预测预后不良,促进细胞增殖。
IF 2.8 3区 生物学
BMC Molecular and Cell Biology Pub Date : 2022-06-30 DOI: 10.1186/s12860-022-00424-7
Kun Wang, Jian He, Changling Tu, Hui Xu, Xugang Zhang, Yongchang Lv, Chao Song
{"title":"Upregulation of CCT3 predicts poor prognosis and promotes cell proliferation via inhibition of ferroptosis and activation of AKT signaling in lung adenocarcinoma.","authors":"Kun Wang,&nbsp;Jian He,&nbsp;Changling Tu,&nbsp;Hui Xu,&nbsp;Xugang Zhang,&nbsp;Yongchang Lv,&nbsp;Chao Song","doi":"10.1186/s12860-022-00424-7","DOIUrl":"https://doi.org/10.1186/s12860-022-00424-7","url":null,"abstract":"<p><strong>Background: </strong>Chaperonin containing TCP1 subunit 3 (CCT3) acts as an oncogene in cancers, whereas its role and underlying mechanisms in lung adenocarcinoma (LUAD) are poorly understood. This study investigated the clinical relevance and function of CCT3 in LUAD.</p><p><strong>Methods: </strong>Clinical relevance of CCT3 in LUAD and lung squamous cell carcinoma (LUSC) was analyzed based on TCGA database. qRT-PCR and Western blot were used to detect mRNA and protein expression, respectively. CCK8 and colony formation were performed to measure cell viability. PI and PI/Annexin V-FITC assay kit was used to determine cell cycle and cell death, respectively. Luciferase activity was performed to check whether CCT3 regulated slc7a11's transcription activity. Ferroptosis was determined by incubating the cells with ferroptosis and apoptosis inducer, their inhibitor and autophagy inhibitor, followed by cell viability examination.</p><p><strong>Results: </strong>We found that CCT3 was overexpressed in LUAD and LUSC tissues. Overexpression of CCT3 predicted the poor prognosis of LUAD patients. Loss-of-function and gain-of-function experiments demonstrated that CCT3 promoted the proliferation and colony formation of LUAD cells. In addition, CCT3 promoted cell cycle progression and suppressed slc7a11-mediated cell ferroptosis, but not apoptosis. We also found that CCT3 activated AKT. MK2206 significantly reduced the viability of CCT3 overexpressed LUAD cells, while had smaller inhibitory effect on the proliferation of control cells, suggesting that CCT3 dictates the sensitivity of LUAD cells to AKT inhibition.</p><p><strong>Conclusion: </strong>Our study demonstrates that CCT3 contributes to the proliferation and growth of LUAD cells through inhibition of ferroptosis and activation of AKT.</p>","PeriodicalId":9099,"journal":{"name":"BMC Molecular and Cell Biology","volume":null,"pages":null},"PeriodicalIF":2.8,"publicationDate":"2022-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9245217/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40552218","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 5
Decreased Krüppel-like factor 4 in adenomyosis impairs decidualization by repressing autophagy in human endometrial stromal cells. 子宫腺肌病患者kr<s:1> ppel样因子4的降低通过抑制人子宫内膜基质细胞的自噬而损害去细胞化。
IF 2.8 3区 生物学
BMC Molecular and Cell Biology Pub Date : 2022-06-27 DOI: 10.1186/s12860-022-00425-6
Jie Mei, Xiaoqiang Sheng, Yuan Yan, Xinyu Cai, Chunxue Zhang, Jiao Tian, Mei Zhang, Jidong Zhou, Huizhi Shan, Chenyang Huang
{"title":"Decreased Krüppel-like factor 4 in adenomyosis impairs decidualization by repressing autophagy in human endometrial stromal cells.","authors":"Jie Mei,&nbsp;Xiaoqiang Sheng,&nbsp;Yuan Yan,&nbsp;Xinyu Cai,&nbsp;Chunxue Zhang,&nbsp;Jiao Tian,&nbsp;Mei Zhang,&nbsp;Jidong Zhou,&nbsp;Huizhi Shan,&nbsp;Chenyang Huang","doi":"10.1186/s12860-022-00425-6","DOIUrl":"https://doi.org/10.1186/s12860-022-00425-6","url":null,"abstract":"<p><strong>Background: </strong>Poor decidualization and abnormal autophagy conditions in the endometria of adenomyosis patients have been reported previously. However, the specific regulatory mechanism of decidualization in adenomyosis and its relationship with autophagy levels have not been clarified.</p><p><strong>Methods: </strong>Endometrial tissues from adenomyosis patients and uteri from an adenomyosis mouse model were collected for the detection of different expression patterns of KLF4 and autophagy markers (LC3-B/LC3-A and Beclin-1) compared with control groups. Human endometrial stromal cells (hESCs) isolated from adenomyosis and control endometrial tissues were employed to elucidate the biological functions of KLF4 in autophagy and decidualization. Gene expression regulation was examined by quantitative real-time PCR (qRT-PCR), western blotting and luciferase reporter assays. In addition, DNA promoter-protein interactions were examined by chromatin immunoprecipitation (ChIP)/PCR assay and avidin-biotin conjugate DNA precipitation (ABCD) assay.</p><p><strong>Results: </strong>KLF4 expression was decreased in endometrial tissues from adenomyosis patients compared with those from fertile controls, especially in stromal compartments. The opposite results were observed for autophagy marker (LC3-B/LC3-A and Beclin-1) expression. At the same time, KLF4 reversed the poor decidualization of hESCs from adenomyosis patients. In addition, KLF4 could induce hESC decidualization by promoting the autophagy level. Mechanistically, KLF4 bound to a conserved site in the autophagy-related 5 (ATG5) promoter region and promoted ATG5 expression. Similar expression patterns of KLF4 and autophagy markers were detected in adenomyotic mice.</p><p><strong>Conclusions: </strong>KLF4 overexpression increases the autophagy level of hESCs by transcriptionally promoting ATG5 expression, and abnormally decreased KLF4 in adenomyosis impairs hESC decidualization by repressing autophagy.</p>","PeriodicalId":9099,"journal":{"name":"BMC Molecular and Cell Biology","volume":null,"pages":null},"PeriodicalIF":2.8,"publicationDate":"2022-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9238063/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40404290","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 3
Evaluation of the putative lymphoma-associated point mutation D427H in the STAT3 transcription factor. STAT3转录因子中疑似淋巴瘤相关点突变D427H的评估。
IF 2.8 3区 生物学
BMC Molecular and Cell Biology Pub Date : 2022-06-25 DOI: 10.1186/s12860-022-00422-9
Lena Sophie Behrendsen, Priyanka Rajeev Menon, Muhammad Jawad Khan, Anke Gregus, Oliver Wirths, Thomas Meyer, Julia Staab
{"title":"Evaluation of the putative lymphoma-associated point mutation D427H in the STAT3 transcription factor.","authors":"Lena Sophie Behrendsen,&nbsp;Priyanka Rajeev Menon,&nbsp;Muhammad Jawad Khan,&nbsp;Anke Gregus,&nbsp;Oliver Wirths,&nbsp;Thomas Meyer,&nbsp;Julia Staab","doi":"10.1186/s12860-022-00422-9","DOIUrl":"https://doi.org/10.1186/s12860-022-00422-9","url":null,"abstract":"<p><strong>Background: </strong>Signal transducer and activator of transcription 3 (STAT3) is an oncogenic transcription factor that promotes cell proliferation and immunomodulation in untransformed cells and maintains stemness of transformed cells, facilitating invasion and metastasis. Numerous point mutations in the STAT3 protein have been identified that drive malignancy in various tumor entities. The missense mutation D427H localized in the STAT3 DNA-binding domain has been previously reported in patients with NK/T cell lymphomas. To assess the biological activity of this missense mutation, we compared the STAT3-D427H mutant to wild-type (WT) protein as well as the known hyper-active mutant F174A.</p><p><strong>Results: </strong>Although previously reported as an activating mutation, the STAT3-D427H mutant neither showed elevated cytokine-induced tyrosine phosphorylation nor altered nuclear accumulation, as compared to the WT protein. However, the D427H mutant displayed enhanced binding to STAT-specific DNA-binding sites but a reduced sequence specificity and dissociation rate from DNA, which was demonstrated by electrophoretic mobility shift assays. This observation is consistent with the phenotype of the homologous E421K mutation in the STAT1 protein, which also displayed enhanced binding to DNA but lacked a corresponding increase in transcriptional activity.</p><p><strong>Conclusions: </strong>Based on our data, it is unlikely that the D427H missense mutation in the STAT3 protein possesses an oncogenic potential beyond the WT molecule.</p>","PeriodicalId":9099,"journal":{"name":"BMC Molecular and Cell Biology","volume":null,"pages":null},"PeriodicalIF":2.8,"publicationDate":"2022-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9233852/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40399837","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
OSGIN2 regulates osteogenesis of jawbone BMSCs in osteoporotic rats. OSGIN2对骨质疏松大鼠颌骨间充质干细胞成骨的调控作用。
IF 2.8 3区 生物学
BMC Molecular and Cell Biology Pub Date : 2022-06-21 DOI: 10.1186/s12860-022-00423-8
Yi Shuai, Bingyao Liu, Liang Rong, Bingyi Shao, Bo Chen, Lei Jin
{"title":"OSGIN2 regulates osteogenesis of jawbone BMSCs in osteoporotic rats.","authors":"Yi Shuai,&nbsp;Bingyao Liu,&nbsp;Liang Rong,&nbsp;Bingyi Shao,&nbsp;Bo Chen,&nbsp;Lei Jin","doi":"10.1186/s12860-022-00423-8","DOIUrl":"https://doi.org/10.1186/s12860-022-00423-8","url":null,"abstract":"<p><strong>Background: </strong>Augmentation of oxidative stress after estrogen deficiency leading to functional deficiency of jawbone bone marrow mesenchymal stem cells (BMSCs) causes jawbone loss in osteoporosis. OSGIN2, an oxidative stress induced factor, has been found to be associated with skeletal diseases. This study aims to investigate the function of OSGIN2 in jawbone BMSCs of osteoporotic rats. Jawbone BMSCs were used.</p><p><strong>Results: </strong>Oxidative stress was increased in jawbone BMSCs of osteoporotic rats, meanwhile OSGIN2 was also up-regulated. Osteogenesis of jawbone BMSCs was declined under oxidative stress, while silence of OSGIN2 ameliorated the osteogenic deficiency. RORα and its downstream osteogenic markers (BSP and OCN) decreased under oxidative stress, while knocking-down of OSGIN2 restored their expressions. Inhibition of OSGIN2 improved the osteogenesis of jawbone BMSCs under oxidative stress, whereas down-regulation of RORα offset the effect. Intra-jawbone infusion of si-OSGIN2 rescued jawbone loss and promoted new bone deposition of osteoporotic rats.</p><p><strong>Conclusions: </strong>Oxidative stress is redundant in osteoporosis, which results in up-regulation of OSGIN2. OSGIN2 restricts osteogenic ability of jawbone BMSCs via regulating RORα, while silencing of OSGIN2 rescues the osteogenic deficiency of osteoporotic rats.</p>","PeriodicalId":9099,"journal":{"name":"BMC Molecular and Cell Biology","volume":null,"pages":null},"PeriodicalIF":2.8,"publicationDate":"2022-06-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9215015/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40164232","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
RNAP II produces capped 18S and 25S ribosomal RNAs resistant to 5′-monophosphate dependent processive 5′ to 3′ exonuclease in polymerase switched Saccharomyces cerevisiae 在聚合酶开关的酿酒酵母中,RNAP II产生抗5 ' -单磷酸依赖的5 '到3 '过程核酸外切酶的18S和25S核糖体rna
IF 2.8 3区 生物学
BMC Molecular and Cell Biology Pub Date : 2022-04-10 DOI: 10.1186/s12860-022-00417-6
Miguel A. Rocha, B. S. Gowda, J. Fleischmann
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