BMC Molecular and Cell Biology最新文献

{"title":"Immune gene expression in salmon keratocytes upon bacterial exposure.","authors":"Marie Kristin Mikkelborg, Antonette Skram Helgestad, Roy Ambli Dalmo, Dhivya Borra Thiyagarajan","doi":"10.1186/s12860-025-00553-9","DOIUrl":"10.1186/s12860-025-00553-9","url":null,"abstract":"","PeriodicalId":9099,"journal":{"name":"BMC Molecular and Cell Biology","volume":"26 1","pages":"28"},"PeriodicalIF":2.7,"publicationDate":"2025-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12462119/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145136425","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rescue of ciliogenesis and hyperglutamylation mutant phenotype in AGBL5^-/- cell model of retinitis pigmentosa.","authors":"Suly S Villa-Vasquez, Liliya Nazlamova, Reuben J Pengelly, David I Wilson, Diana Baralle, Gabrielle Wheway","doi":"10.1186/s12860-025-00551-x","DOIUrl":"10.1186/s12860-025-00551-x","url":null,"abstract":"<p><p>Retinitis pigmentosa (RP) affects around 1 in 4000 individuals and represents approximately 25% of cases of vision loss in adults, through death of retinal rod and cone photoreceptor cells. It remains a largely untreatable disease, and research is needed to identify potential targets for therapy. Mutations in 94 different genes have been identified as causing RP, including AGBL5 which encodes the main deglutamylase that regulates and maintains functional levels of cilia tubulin glutamylation, which is essential to initiate ciliogenesis, maintain cilia stability and motility. In this study we use CRISPR-mutated AGBL5 clonal retinal pigmented epithelial cell lines to characterise the cilia defects and hyperglutamylation in these cells and identify potential targets for treatment. We demonstrate rescue of glutamylation to wild-type levels and restoration of ciliogenesis in AGBL5 mutant cells through exogenous expression of AGBL5, and independently through both stable genomic mutation and transient siRNA knockdown of TTLL5, which encodes a tubulin glutamylase. This identifies two potential routes to treatment for patients with RP associated with mutations in AGBL5 which will need to be explored further in retinal organoid models of this disease.</p>","PeriodicalId":9099,"journal":{"name":"BMC Molecular and Cell Biology","volume":"26 1","pages":"27"},"PeriodicalIF":2.7,"publicationDate":"2025-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12418683/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145028907","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evolutionary and structural insights into DNMTs and TETs: decoding their functional heterogeneity and oncogenic roles in methylation regulation.","authors":"Siqi Yang, Xinyi Li, Lingling Bao, Jiafu Cui, Jing Liu, Shan Cong, Yongchun Zuo","doi":"10.1186/s12860-025-00552-w","DOIUrl":"https://doi.org/10.1186/s12860-025-00552-w","url":null,"abstract":"<p><p>DNA methylation in mammals is dynamically regulated by DNMTs and TETs. Despite their critical roles, comparative structural analyses of these protein families have been relatively scarce. To address the above problems, this study first constructed a phylogenetic tree of DNMT and TET proteins to investigate their evolutionary relationships. Furthermore, the structural exploration revealed that both protein families possess conserved β-sheet structures and exhibit the characteristics of alternating β-sheets in their catalytic domains. Interestingly, DNMTs contain more α helices and fewer loops compared to TETs. Several notable structural changes were discovered, including unique flexibility of the CXXC domain and divergences in DNA binding mechanisms among DNMT1, TET1, and TET3. Additionally, the results showed that a distinctive loop present in DNMT2 may indicate its specialized functional role. This research provides fundamental evolutionary and structural insights into DNMT and TET proteins, emphasizing their significance in tissue-specific distribution and cancer signaling, thereby establishing a foundation for future investigations in the field of epigenetics.</p>","PeriodicalId":9099,"journal":{"name":"BMC Molecular and Cell Biology","volume":"26 1","pages":"26"},"PeriodicalIF":2.7,"publicationDate":"2025-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12392533/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144942601","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cell biology of Dictyostelium.","authors":"Robert J Huber, Paul A Steimle, Cynthia K Damer","doi":"10.1186/s12860-025-00550-y","DOIUrl":"10.1186/s12860-025-00550-y","url":null,"abstract":"<p><p>The Cell Biology of Dictyostelium article collection highlights the benefits of using Dictyostelium as a model system for studying fundamental cellular processes. The studies compiled in this collection showcase the many ways Dictyostelium is used to enhance our understanding of the eukaryotic cell.</p>","PeriodicalId":9099,"journal":{"name":"BMC Molecular and Cell Biology","volume":"26 1","pages":"25"},"PeriodicalIF":2.7,"publicationDate":"2025-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12362907/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144882103","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Indian medicinal phytocompounds for targeting apoptosis and high-penetrance genes in triple-negative breast cancer: an in-silico exploration.","authors":"Reshmi Kumari, Satarupa Banerjee","doi":"10.1186/s12860-025-00548-6","DOIUrl":"10.1186/s12860-025-00548-6","url":null,"abstract":"<p><p>Triple-negative breast cancer (TNBC) presents a significant therapeutic challenge due to its aggressive nature, lack of hormone receptors, and limited targeted treatment options. The complexity of the disease is further compounded by mutations in high-penetrance genes such as BRCA1, BRCA2, and BAX, along with other apoptotic genes involved in tumorigenesis, apoptosis, and drug resistance. Targeting these genes through innovative therapeutic approaches is crucial for improving treatment outcomes. This in-silico study explores the potential of phytochemicals as natural, multi-targeted therapeutic agents against high-penetrance and apoptotic genes implicated in TNBC. Using the IMPPAT 2.0 database, 300 phytochemicals were systematically screened based on their pharmacokinetic properties and toxicity profiles to identify promising candidates. Among them, Bayogenin exhibited strong binding to BRCA2 (-9.3 kcal/mol) and PALB2 (-8.7 kcal/mol), surpassing the FDA-approved drug Olaparib in molecular docking studies. Molecular dynamics simulations over 200 ns further confirmed the stability of these phytochemical-protein complexes, showing consistent root mean square deviation, hydrogen bonding, and free energy profiles. These findings highlight the therapeutic potential of phytochemicals and their possible advantages over existing TNBC treatments. By targeting key molecular pathways, this study provides insights into the development of natural, multi-targeted therapeutic strategies, emphasizing their translational relevance for TNBC therapy.</p>","PeriodicalId":9099,"journal":{"name":"BMC Molecular and Cell Biology","volume":"26 1","pages":"24"},"PeriodicalIF":2.7,"publicationDate":"2025-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12312594/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144752258","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In vivo genetic labeling of primary cilia in developing astrocytes.","authors":"Rachel Bear, Claire Wei, Tamara Caspary","doi":"10.1186/s12860-025-00547-7","DOIUrl":"10.1186/s12860-025-00547-7","url":null,"abstract":"<p><strong>Background: </strong>Astrocyte cilia are largely understudied due to the lack of available tools. Astrocyte research advanced with the establishment of Aldh1l1-Cre^ERT2, an inducible Cre line that specifically targets the astrocyte lineage. Here, we develop and compare genetic models that label astrocyte cilia in the developing prefrontal cortex (PFC) using Aldh1l1-Cre^ERT2 and Cre-dependent cilia reporters. We evaluate these models by testing different tamoxifen-induction protocols and quantifying the percentage of astrocytes labeled with the cilia reporters.</p><p><strong>Results: </strong>We show that tamoxifen dosage impacts the expression of cilia reporters in astrocytes. We uncover the maximum cilia-labeling efficiency using recombined, constitutively-expressed cilia reporters.</p><p><strong>Conclusion: </strong>The data reveal that only a subset of SOX9- positive astrocytes in the PFC possess cilia throughout development. Our work highlights the utility of Cre-Lox systems to target specific cell types and the importance of carefully validating genetic models.</p>","PeriodicalId":9099,"journal":{"name":"BMC Molecular and Cell Biology","volume":"26 1","pages":"23"},"PeriodicalIF":2.7,"publicationDate":"2025-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12288362/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144706239","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Methodological approach for allele-specific antibody responses to HEK-293T-based cell lines expressing single MHC class I chain-related gene B antigens.","authors":"Ji-Ho Jeon, Cheol-Hwa Hong, You-Seok Hyun, Hyeyoung Lee, Eun-Jee Oh, Tai-Gyu Kim, In-Cheol Baek","doi":"10.1186/s12860-025-00549-5","DOIUrl":"10.1186/s12860-025-00549-5","url":null,"abstract":"<p><strong>Background: </strong>Antibodies against non-HLA antigens, such as MICA and MICB, have emerged as potential contributors to antibody-mediated rejection and graft failure. While MICA antibodies are well characterized, MICB-specific antibodies remain poorly understood due to the lack of standardized detection tools. To address this gap, we aimed to develop a cell-based platform expressing individual MICB antigens to evaluate the feasibility of detecting allele-specific anti-MICB antibodies in pre-kidney transplant sera.</p><p><strong>Methods: </strong>HLA class I, MICA, and MICB-null human embryonic kidney (HEK)-293T cells were previously generated by CRISPR/Cas9. We established five cell lines expressing single MICB antigens (each MICB*002, *003, *004, *005:02, and *008 allele). A total of 64 pre-kidney transplant sera were tested to assess anti-MICB antibody responses to the five cell lines using flow cytometry.</p><p><strong>Results: </strong>We successfully established and validated five HEK-293T cell lines each expressing a single MICB antigen using anti-MICB monoclonal antibody staining. No anti-MICB antibodies were detected in any of the 64 pre-transplant sera tested. This finding may reflect a low incidence of sensitization to MICB in this patient population and suggests the need for larger, more diverse cohorts in future studies to fully assess the prevalence of anti-MICB responses. The established cell lines provide a promising tool for future investigation of allele-specific anti-MICB antibody responses.</p><p><strong>Conclusions: </strong>While the present study did not detect allele-specific anti-MICB antibody responses, establishing HEK-293T cell lines expressing single MICB antigens represents a significant methodological advance. This platform enables the potential assessment of immune responses targeted to individual MICB allotypes, thus offering new avenues for the future study of MICB immunogenicity in transplantation settings.</p>","PeriodicalId":9099,"journal":{"name":"BMC Molecular and Cell Biology","volume":"26 1","pages":"22"},"PeriodicalIF":2.4,"publicationDate":"2025-07-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12265220/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144648435","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"circFTO binding with IGF2BP2 regulates trophoblast cells proliferation, migration, and invasion while mediating m6A modification of CCAR1 mRNA in spontaneous abortion.","authors":"Yiwen Zhang, Meiyao Wu, Xiaoling Lin, Bingfeng Lu, Xi Chen, Qianhui Li, Yinan Jiang, Baixue Li, Dongmei Zhou, Xiujie Sheng","doi":"10.1186/s12860-025-00546-8","DOIUrl":"10.1186/s12860-025-00546-8","url":null,"abstract":"<p><strong>Background: </strong>Spontaneous abortion (SA) is a complex reproductive disease that poses significant clinical challenge. Circular RNAs (circRNAs), a specific class of endogenous non-coding RNAs, hold significant potential for preclinical diagnosis and therapeutic interventions in various diseases. However, the precise roles of circRNAs in SA have yet to be fully elucidated.</p><p><strong>Methods: </strong>In this study, we employed RNA sequencing and quantitative real-time polymerase chain reaction (qRT-PCR) to identify an upregulated circRNA, circFTO (hsa_circ_0005941), in the placental villi of SA patients. We conducted in vitro and in vivo experiments to ascertainthe functional significance of circFTO in trophoblast cell lines. The molecular mechanisms associated with circFTO were predicted using online databases and confirmed through RNA immunoprecipitation (RIP) assays, Western blotting, and rescue experiments.Additionally, Actinomycin D was employed to assess changes in the stability of target messenger RNA (mRNA) under different treatments. Furthermore, colorimetric examinations were used to evaluate the m6A methylation levels of trophoblast cells, and meRIP-qPCR assays confirmed the m6A modification of CCAR1 mRNA.</p><p><strong>Results: </strong>Our findings revealed that circFTO was upregulated in the placenta of SA patients. Functionally, downregulating circFTO expression enhanced trophoblast cell proliferation, migration, and invasion. Conversely, overexpression of circFTO inhibited these functions in trophoblast cells. Trophoblast organoids derived from normal pregnancies exhibited reduced proliferation upon overexpression of circFTO. Bioinformatics prediction and subsequent experiments demonstrated that circFTO directly bound to and negatively regulated IGF2BP2. Reducing the level of IGF2BP2 partially restored the alterations in trophoblast function caused by circFTO knockdown. Colorimetric assay, RNA decay experiments, and meRIP-qRT-PCR analysis revealed that circFTO knockdown increased m6A methylation levels in CCAR1 mRNA, while circFTO overexpression decreased m6A methylation levels. This modification is known to play a crucial role in Zygotic gene activation.</p><p><strong>Conclusions: </strong>Our study unveils the pivotal functions of circFTO within trophoblasts and elucidates a unique circFTO-IGF2BP2-CCAR1 axis, which may hold significant potential as diagnostic and therapeutic targets for the treatment of SA.</p><p><strong>Clinical trial number: </strong>Not applicable.</p>","PeriodicalId":9099,"journal":{"name":"BMC Molecular and Cell Biology","volume":"26 1","pages":"21"},"PeriodicalIF":2.4,"publicationDate":"2025-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12220032/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144552168","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Anti-Inflammatory effects of Lactobacillus helveticus and Arthrospira platensis on colonic cells inflamed by Crohn's disease-associated Escherichia coli.","authors":"Samira Alipour, Hossein Halimi, Nastaran Asri, Mohammad Rostami-Nejad, Leila Pishkar, Hamidreza Houri","doi":"10.1186/s12860-025-00545-9","DOIUrl":"10.1186/s12860-025-00545-9","url":null,"abstract":"<p><strong>Background: </strong>Adherent-invasive Escherichia coli (AIEC) is linked to intestinal inflammation in inflammatory bowel disease (IBD). Arthrospira platensis and Lactobacillus helveticus exhibit anti-inflammatory properties individually, yet their effects remain underexplored in IBD-associated inflammation. We aimed to investigate the anti-inflammatory potential of L. helveticus and the hydroalcoholic extract of A. platensis (HA-A. platensis) in Caco-2 cells inflamed by IBD-associated E. coli.</p><p><strong>Methods: </strong>Caco-2 cells inflamed by a Crohn's disease (CD)-associated E. coli strain (MOI 10) were treated with HA-A. platensis (2 mg/mL) and/or L. helveticus (MOI 50) in live (LBC), heat-killed (HKC), or cell-free supernatant (CFS) forms. The anti-invasion/adhesion properties of L. helveticus and/or HA-A. platensis were investigated by assessing the CD-associated E. coli invasion/adhesion rate (%). Signaling molecules (NF-κB, STAT3, NOD2) were analyzed via qPCR to capture pathway activation dynamics, while cytokines (TNF-α, IL-1β, IL-8, IL-10) were quantified by ELISA to assess secreted functional proteins.</p><p><strong>Results: </strong>HA-A. platensis reduced E. coli adhesion by 68% (P < 0.001) and completely inhibited invasion. L. helveticus (live form) decreased adhesion by 88% and invasion by 90%. Combined treatment showed synergistic effects, reducing adhesion by 89% and fully blocking invasion. HA-A. platensis downregulated STAT3 expression by 0.4-fold (P < 0.01), while L. helveticus (heat-killed form) reduced NF-κB by 0.51-fold (P < 0.05) and increased NOD2 by 1.8-fold (P < 0.01). Cytokine analysis revealed that HA-A. platensis decreased IL-1β by 0.61-fold (P < 0.001), and L. helveticus (heat-killed) reduced TNF-α (0.51-fold) and IL-8 (0.23-fold) while elevating anti-inflammatory IL-10 (4.39-fold; P < 0.001).</p><p><strong>Conclusions: </strong>L. helveticus and HA-A. platensis synergistically inhibit CD-associated E. coli pathogenicity and modulate inflammatory responses in vitro. These findings highlight their potential as adjunctive therapies for CD, warranting further preclinical validation.</p>","PeriodicalId":9099,"journal":{"name":"BMC Molecular and Cell Biology","volume":"26 1","pages":"20"},"PeriodicalIF":2.4,"publicationDate":"2025-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12160103/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144274212","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sex alters thyroid hormone's effect on protein O-GlcNAcylation in the aged mouse heart.","authors":"Aaron K Olson, Wei Zhong Zhu, Dolena Ledee","doi":"10.1186/s12860-025-00543-x","DOIUrl":"10.1186/s12860-025-00543-x","url":null,"abstract":"<p><strong>Background: </strong>The aging heart undergoes physiological changes, many of which are sex dependent and encompass differential responses to cardiac stress. However, much about the molecular changes that occur within the aging heart is still unknown. Thyroid hormone (TH) and the posttranslational modification O-GlcNAcylation (O-GlcNAc) are independently known to regulate cardiac function; therefore, we tested the hypothesis that TH disorders affect cardiac protein O-GlcNAcylation in aged hearts.</p><p><strong>Results: </strong>We treated male and female 18-22 month-old aged C57BL/6 mice to create euthyroid, hypothyroid, or hyperthyroid states. Western blots and RT-qPCR from cardiac tissue were used to determine changes in global O-GlcNAc levels along with key regulatory proteins in the O-GlcNAcylation process. Immunoprecipitation and western blotting compared global O-GlcNAc changes to differences on an individual protein. We found increased total O-GlcNAc levels for female hypo- and hyperthyroid mice and male hyperthyroid mice compared to sex-matched euthyroid hearts, with no change for male hypothyroid mice. TH's O-GlcNAc effect on female mice appears heart specific as liver O-GlcNAc levels were unchanged. The proteins regulating O-GlcNAcylation also demonstrated sex differences. Female hyperthyroid mice had increased protein expression of the O-GlcNAc regulatory proteins GFAT 1, GFAT 2, and OGT, whereas the hyperthyroid male mice showed decreased expression for the regulatory protein OGA. The hypothyroid female mice had increased protein expression for OGT and NAGK, whereas the hypothyroid male mice showed increased protein expression for NAGK alone. Interestingly, the directional changes in these protein levels did not match RNA transcription. We further found O-GlcNAc levels of the mitochondrial thiolase protein ACAA2 diverged from global O-GlcNAc changes. ACAA2 was hyper O-GlcNAcylated in the female hypothyroid group and hypo O-GlcNAcylated in the male hyperthyroid group whereas there was no change in female hyperthyroid or male hypothyroid.</p><p><strong>Conclusion: </strong>Protein O-GlcNAcylation is potentially an important mechanism whereby TH perturbations affect the aged heart. We found sex influences O-GlcNAc regulation, global O-GlcNAc levels, and O-GlcNAc protein specificity in response to thyroid hormone perturbations. Our results also suggest the changes in cardiac O-GlcNAc levels are not solely due to TH transcriptional regulation of key O-GlcNAc regulatory enzymes.</p>","PeriodicalId":9099,"journal":{"name":"BMC Molecular and Cell Biology","volume":"26 1","pages":"19"},"PeriodicalIF":2.4,"publicationDate":"2025-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12150571/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144265229","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}