BMC Molecular and Cell Biology最新文献

{"title":"Nutrient and heat stress induce changes to the solubility of predicted prion-like proteins in Dictyostelium discoideum.","authors":"Felicia N Williams, Kanesha Travis, Yaneli Guerra-Hernandez, Erik Soderblom, K Matthew Scaglione","doi":"10.1186/s12860-026-00582-y","DOIUrl":"10.1186/s12860-026-00582-y","url":null,"abstract":"<p><strong>Background: </strong>Dictyostelium discoideum has a unique proteome among sequenced organisms that encodes nearly 10,000 homopolymeric amino acid tracts longer than 10 amino acids long. These repeats are composed of every amino acid except tryptophan with asparagine and glutamine being the most prevalent. Interestingly, asparagine and glutamine-rich regions of proteins are the hallmark of prion-like domains and nearly 25% of the Dictyostelium proteome is predicted to be prion-like in nature.</p><p><strong>Results: </strong>Here we assessed the insoluble proteome upon heat stress or during nutrient stress which induces Dictyostelium development. We found that both heat and nutrient stress induce the accumulation of predicted prion-like proteins in the insoluble fraction; however, the overall amino acid composition of insoluble proteins is different depending on the stress with a greater percentage of predicted prion-like proteins becoming insoluble upon nutrient stress. We further confirmed that endogenous polyglutamine proteins accumulate in the insoluble fraction over the developmental time course and demonstrate that exogenously expressed polyglutamine tracts form puncta and have reduced solubility in a polyglutamine length dependent manner upon nutrient stress. Finally, using a proximity labeling approach, we found that exogenously expressed polyglutamine tracts have enhanced proximity to glutamine-rich proteins.</p><p><strong>Conclusion: </strong>During heat and nutrient stress Dictyostelium have numerous proteins that have decreased solubility. Among these proteins, predicted prion-like proteins are enriched and the presence of a polyglutamine tract is sufficient to cause decreased solubility in a polyglutamine length dependent manner.</p>","PeriodicalId":9099,"journal":{"name":"BMC Molecular and Cell Biology","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2026-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13093961/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147442815","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Regulation of tRNA expression during the social cycle of the amoeba Dictyostelium discoideum.","authors":"Dulce I Valdivia, Peter F Stadler","doi":"10.1186/s12860-026-00580-0","DOIUrl":"10.1186/s12860-026-00580-0","url":null,"abstract":"","PeriodicalId":9099,"journal":{"name":"BMC Molecular and Cell Biology","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2026-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13063481/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147372283","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Splicing isoforms associated with TGFβ-induced myofibroblast activation.","authors":"Opeoluwa Alli-Oke, Danny Bergeron, Fiona Kessai, Philippe Thibault, Mathieu Durand, Jean-Philippe Brosseau","doi":"10.1186/s12860-026-00579-7","DOIUrl":"10.1186/s12860-026-00579-7","url":null,"abstract":"","PeriodicalId":9099,"journal":{"name":"BMC Molecular and Cell Biology","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2026-03-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13077938/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147364009","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"ALIX and ITCH localize to the base of primary cilia and negatively regulate ciliary Polycystin-2 levels.","authors":"Christina Rahlff Berggreen, Julie Laplace, Fabiola Campestre, Anna-Louise With Petersen, Anna Maria Fixdahl, Benjamin Mary, Saba Ghazanfar, Geyi Li, Lotta Elisabeth Wagner, Csenge K Rezi, Mohamed Chamlali, Zeinab Anvarian, Søren T Christensen, Helen L May-Simera, Lotte B Pedersen","doi":"10.1186/s12860-026-00571-1","DOIUrl":"10.1186/s12860-026-00571-1","url":null,"abstract":"","PeriodicalId":9099,"journal":{"name":"BMC Molecular and Cell Biology","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2026-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13041057/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147302134","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"ATP-related purinergic signaling via P2Y12 contributes to airway epithelial barrier integrity.","authors":"Kota Tsuya, Shuichiro Maruoka, Shinichi Okamoto, Sotaro Shikano, Mari Ujike, Shiho Yamada, Yusuke Kurosawa, Kenji Mizumura, Yutaka Kozu, Yasuhiro Gon","doi":"10.1186/s12860-026-00578-8","DOIUrl":"10.1186/s12860-026-00578-8","url":null,"abstract":"","PeriodicalId":9099,"journal":{"name":"BMC Molecular and Cell Biology","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2026-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13041268/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147302143","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Insulin signaling engages divergent transcriptional mechanisms in neural and metabolic tissues.","authors":"Roshni Jain, Rutuj Kolhe, Cassandra Hui, Juli Petereit, Dennis Mathew","doi":"10.1186/s12860-026-00577-9","DOIUrl":"10.1186/s12860-026-00577-9","url":null,"abstract":"","PeriodicalId":9099,"journal":{"name":"BMC Molecular and Cell Biology","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2026-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13019849/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146225283","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A nanobody-based degron system for targeted protein knockdown in Dictyostelium discoideum.","authors":"Hidenori Hashimura, Shoko Fujishiro, Nao Shimada, Tomoko Adachi, Toyoko Sugita, Satoshi Kuwana, Satoshi Sawai","doi":"10.1186/s12860-026-00572-0","DOIUrl":"10.1186/s12860-026-00572-0","url":null,"abstract":"<p><strong>Backgrounds: </strong>The cellular slime mold Dictyostelium discoideum is a widely used model system for studying basic processes in cell and developmental biology. While genetic tools, such as targeted gene disruption by homologous recombination and genome editing using CRISPR/Cas9, are well-established in D. discoideum, efficient methods for conditional loss-of-function studies are limited. Here, we developed a nanobody-based degron system for D. discoideum based on ALFA-tagged protein recruitment to the Skp1-Cullin-F-box (SCF) complex.</p><p><strong>Results: </strong>ALFA-tagged Histone H1 was efficiently degraded by expressing anti-ALFA nanobody (NbALFA) fused to the D. discoideum FbxD F-box domain ('dictyGrad-ALFA'). Cell type-specific targeting was achieved by expressing dictyGrad-ALFA under prestalk- and prespore-specific gene promoters. Furthermore, targeting of adenylyl cyclase A (ACA) resulted in the expected aggregation-deficient phenotype, validating the efficacy of dictyGrad-ALFA-mediated protein depletion. Cell type-specific ACA degradation delayed development but eventually resulted in normal fruiting bodies. Our ALFA-tag approach was further used for conditional knockdown in combination with the auxin-inducible degron 2 (AID2) system, which relies on indole-3-acetic acid (IAA)-dependent binding between NbALFA-mAID and a OsTIR-F-box-Skp1A fusion protein. We obtained efficient IAA-induced degradation in prestalk cells; however, efficiency was low in other cell types.</p><p><strong>Conclusions: </strong>Together, these systems pave the way for conditional and cell type-specific protein degradation in D. discoideum, enabling functional analyses of genes essential for growth and development.</p>","PeriodicalId":9099,"journal":{"name":"BMC Molecular and Cell Biology","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2026-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13020093/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146225226","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Human urine-derived stem exosomes inhibit inflammation and oxidative stress in sepsis-related acute kidney injury.","authors":"Lulu Liu, Lili Ding, Zhaorui Sun, Weichao Ding, Haijun Sun, Maohong Xia, Quan Li","doi":"10.1186/s12860-026-00575-x","DOIUrl":"10.1186/s12860-026-00575-x","url":null,"abstract":"<p><strong>Objective: </strong>The objective of this study was to explore the mechanisms by which urine - derived stem cell exosomes (USCexo) alleviate sepsis - related acute kidney injury (SAKI), as acute kidney injury often complicates sepsis with unclear role of USCexo in SAKI while stem cell exosomes show potential in AKI treatment.</p><p><strong>Methods: </strong>Human urine - derived stem cells (USCs) were isolated from human urine and characterized via flow cytometry. A sepsis - related acute kidney injury mouse model was induced by cecal ligation and puncture (CLP). The mice were divided into a control group receiving phosphate - buffered saline (PBS) and an experimental group receiving USCexo via tail vein injection. Survival rate, renal damage evaluated by HE staining, and renal function assessed by sCr and BUN levels were measured. Renal RNA was extracted to analyze apoptosis and oxidative stress. In vitro, qRT - PCR was used to assess the inflammatory response in macrophages treated with USCexo.</p><p><strong>Results: </strong>Treatment with USC - derived exosomes led to a reduction in the levels of pathological injury, sCr, and BUN. It inhibited renal cell apoptosis and oxidative stress, decreased the infiltration of inflammatory cells, and protected renal function in SAKI mice. Additionally, USCexo suppressed the inflammatory response of primary peritoneal macrophages induced by lipopolysaccharide (LPS).</p><p><strong>Conclusions: </strong>USC - derived exosomes protect against SAKI by inhibiting oxidative stress and inflammation, which provides a theoretical basis for the potential use of USCexo in the treatment of SAKI.</p><p><strong>Clinical trial number: </strong>Not applicable.</p>","PeriodicalId":9099,"journal":{"name":"BMC Molecular and Cell Biology","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2026-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13014871/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146212219","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dose-dependent effects of atorvastatin on inflammatory response in endothelial cells: MMP/TIMP balance and in vitro wound closure assay.","authors":"Betül İşiner, Aslı Fahriye Ceylan, Büşra Görgün, Leyla Didem Kozacı","doi":"10.1186/s12860-026-00573-z","DOIUrl":"10.1186/s12860-026-00573-z","url":null,"abstract":"<p><strong>Background: </strong>Clinical and preclinical evidence suggests that inflammation is closely associated with various arterial diseases. Multiple studies have reported the effects of statins on different cell types, yet the effects of atorvastatin (ATV) and its mechanisms on inflamed HUVECs' cellular responses are still under investigation. This study investigates how different doses of ATV affect inflammatory responses, extracellular matrix (ECM) regulators, and cell migration capacity assessed by an in vitro scratch wound closure assay in lipopolysaccharide (LPS) stimulated endothelial cells.</p><p><strong>Methods: </strong>ATV was applied to cells at different doses (5-10-50 µM) with or without LPS (20 ng/mL). Cell proliferation and toxicity were investigated in the indicated groups. Then, the possible effects of ATV on MMP-2, MMP-9, TIMP-1,TIMP-2 expression levels, scratch-closure (cell migration) time were examined. Gene expression of MMP-2, MMP-9, TIMP-1, and TIMP-2 was quantified by qPCR, while protein levels were determined by Western blotting. Cell migration was evaluated using a scratch assay and real-time imaging system.</p><p><strong>Results: </strong>High-dose ATV was more cytotoxic, while wound closure times showed a numerical increase that did not reach statistical significance under LPS stimulation. While low-dose ATV increased MMP and TIMP expressions, high-dose treatment reduced TIMP-1 and disturbed the MMP/TIMP balance. This imbalance was accompanied by reduced cellular recovery capacity under inflammatory stress.</p><p><strong>Conclusions: </strong>This study highlights the complex cellular effects of ATV under inflammatory conditions, supporting its context- and dose-dependent role in endothelial cell behavior and matrix regulation. These findings highlight the importance of dosage optimization in therapeutic contexts targeting vascular inflammation and provide novel insight into how statin dosing influences endothelial recovery mechanisms, beyond cholesterol regulation.</p>","PeriodicalId":9099,"journal":{"name":"BMC Molecular and Cell Biology","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2026-02-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13011320/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146206687","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of prey quality through the life cycle of the social amoeba Dictyostelium discoideum.","authors":"Heng Liang, Margaret I Steele, Kaifeng Hu, Steven M Wolf, David C Queller, Joan E Strassmann","doi":"10.1186/s12860-026-00574-y","DOIUrl":"10.1186/s12860-026-00574-y","url":null,"abstract":"","PeriodicalId":9099,"journal":{"name":"BMC Molecular and Cell Biology","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2026-02-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13011474/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146206676","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}