Brazilian Journal of Microbiology最新文献_第7页

β-Lapachone encapsulated into stealth liposomes: inhibition of biofilm and cell wall thickness of MRSA. β-Lapachone包封隐身脂质体：抑制MRSA生物膜和细胞壁厚度。

IF 1.9 4区生物学

Brazilian Journal of Microbiology Pub Date : 2025-09-01 Epub Date: 2025-06-04 DOI: 10.1007/s42770-025-01701-1

Alyson Mykael Albuquerque Florenço, Sayonara Stéfane Tavares de Moura, Sandrelli Meridiana de Fátima Ramos Dos Santos Medeiros, Hanne Lazla Rafael de Queiroz Macêdo, Luís André de Almeida Campos, Nereide Stela Santos-Magalhães, Isabella Macário Ferro Cavalcanti

引用次数: 0

Labeling Corynebacterium pseudotuberculosis using superfolder green fluorescent protein to evaluate its infection in macrophages. 应用超文件夹绿色荧光蛋白标记假结核棒状杆菌，评价其在巨噬细胞中的感染。

IF 1.9 4区生物学

Brazilian Journal of Microbiology Pub Date : 2025-09-01 Epub Date: 2025-07-09 DOI: 10.1007/s42770-025-01729-3

Xincan Li, Yongfeng Yuan, Hong Lv, Chanyu Wu, Pei Li, Zhiying Wang, Zuoyong Zhou

{"title":"Labeling Corynebacterium pseudotuberculosis using superfolder green fluorescent protein to evaluate its infection in macrophages.","authors":"Xincan Li, Yongfeng Yuan, Hong Lv, Chanyu Wu, Pei Li, Zhiying Wang, Zuoyong Zhou","doi":"10.1007/s42770-025-01729-3","DOIUrl":"10.1007/s42770-025-01729-3","url":null,"abstract":"To develop a visual detection method for Corynebacterium pseudotuberculosis (CP), a Gram-positive and facultative intracellular bacterium that causes chronic suppurative diseases in humans and animals, we constructed a superfolder green fluorescent protein (sfGFP) expression plasmid, pXMJ19-sfGFP, which constitutively expresses sfGFP in CP. The effects of pXMJ19-sfGFP on the biological characteristics of CP were evaluated, and no significant effects on the morphology, growth, or pathogenicity of CP were noticed following sfGFP expression. The stability of pXMJ19-sfGFP was determined through sequential propagation and infection tests in macrophages and mice. pXMJ19-sfGFP was stable within CP in the presence of chloramphenicol; however, certain degree of plasmid loss was noticed without antibiotic selection pressure. The rates of loss of pXMJ19-sfGFP in CP recovered from the macrophages infected with sfGFP-labeled strains of different pathogenicity were similar, indicating the feasibility of sfGFP-labeled CP monitoring during infection of macrophages by bacteria. The rate of macrophage infection with sfGFP-labeled CP with or without phospholipase D (pld) mutation was evaluated using flow cytometry, and the results showed that the rate of macrophage infection by CP was significantly higher than that by mutant CP. Therefore, sfGFP expression in CP is a valuable tool for visual analysis of macrophage infection by CP.","PeriodicalId":9090,"journal":{"name":"Brazilian Journal of Microbiology","volume":" ","pages":"2059-2068"},"PeriodicalIF":1.9,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12350864/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144599416","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The use of high-resolution melting analysis to distinguish feline enteric and feline infectious peritonitis coronaviruses. 利用高分辨率熔融分析区分猫肠道和猫传染性腹膜炎冠状病毒。

IF 1.9 4区生物学

Brazilian Journal of Microbiology Pub Date : 2025-09-01 Epub Date: 2025-07-21 DOI: 10.1007/s42770-025-01742-6

Wei Zhang, Jinglong Luo, Litao Yan, Li Xiao

{"title":"The use of high-resolution melting analysis to distinguish feline enteric and feline infectious peritonitis coronaviruses.","authors":"Wei Zhang, Jinglong Luo, Litao Yan, Li Xiao","doi":"10.1007/s42770-025-01742-6","DOIUrl":"10.1007/s42770-025-01742-6","url":null,"abstract":"Objectives: Feline coronavirus (FCoV) infections are a widespread occurrence among feline populations globally. The FCoV family encompasses two distinct biotypes: feline enteric coronavirus (FECV) and feline infectious peritonitis viruses (FIPV). FECV infections are asymptomatic or result in only mild enteric disease, whereas FIPV can cause a high mortality rate. Distinguishing FIPV from FCoV is crucial for prompt treatment. High resolution Melting (HRM) technology can accurately solve this problem.Methods: In our work, one pair of primers was designed from the 7b gene of FCoV. There are three mutations specific for the same fragment amplification between FECV and FIPV. Thus, these mutations causes different dissociation curves and Tm values.Results: HRM analysis showed that Tm values of FIPV was about 2 °C higher than that of FECV. Our assay had high specificity, sensitivity and reproducibility. We have tested the high accuracy of this method in 11 clinical samples and confirmed it with DNA sequencing. The high-resolution melting curve assay provides an alternative mean to distinguish FIPV from FCoV.Conclusions and relevance: This HRM-based assay provides a rapid (< 2 h), cost-effective alternative to sequencing for discriminating FIPV from FECV. Its high accuracy and suitability for routine diagnostics could significantly improve early FIPV identification, enabling timely therapeutic decisions in veterinary practice.","PeriodicalId":9090,"journal":{"name":"Brazilian Journal of Microbiology","volume":" ","pages":"2081-2086"},"PeriodicalIF":1.9,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12350980/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144682013","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Isolation and characterization of a novel phage targeting Klebsiella pneumoniae K2 capsular type. 一种新型靶向肺炎克雷伯菌K2荚膜型噬菌体的分离与鉴定。

IF 1.9 4区生物学

Brazilian Journal of Microbiology Pub Date : 2025-09-01 Epub Date: 2025-06-06 DOI: 10.1007/s42770-025-01707-9

Rahma Kazdaghli, Donia Mourali, Lucas Mora-Quilis, Pilar Domingo-Calap, Kamel Ben-Mahrez

{"title":"Isolation and characterization of a novel phage targeting Klebsiella pneumoniae K2 capsular type.","authors":"Rahma Kazdaghli, Donia Mourali, Lucas Mora-Quilis, Pilar Domingo-Calap, Kamel Ben-Mahrez","doi":"10.1007/s42770-025-01707-9","DOIUrl":"10.1007/s42770-025-01707-9","url":null,"abstract":"Phage therapy is a promising therapeutic weapon in the fight against multidrug-resistant Klebsiella pneumoniae infections. A Klebsiella phage, KpTRp1, was isolated from wastewater sample collected in Tunisia. It exhibited specific lytic activity against a reference strain of K. pneumoniae serotype K2. Phage isolation and enrichment were performed using the double-layer agar method. It was characterized by transmission electron microscopy, physiological investigation and genomic sequence analysis. Transmission electron micrographs of KpTRp1 showed a myovirus morphology. The optimal multiplicity of infection by KpTRp1 was 0.0001 with a latent period lasted for 30 min. Its rise phase showed a burst period of almost 150 min and released a small burst size of about six plaque-forming units per infected cell. KpTRp1 was stable over a broad pH and temperature range. The phage genome sequencing revealed a linear double-stranded DNA genome of 48,003 base pairs with a G + C content of 48.79%, defining a new species of Sircambvirus genus of Jameshumphriesvirinae subfamily. Among the 68 open reading frames identified, about half were hypothetical proteins with unknown functions, the others being involved in DNA replication and metabolism, phage structure, host recognition and lysis. Remarkably, lytic activity of KpTRp1 against K. pneumoniae serotype K2 was confirmed by genomic analysis of life cycle, making it suitable candidate for phage therapy, especially since no antibiotic resistance, or lysogenic genes were detected in the phage genome.","PeriodicalId":9090,"journal":{"name":"Brazilian Journal of Microbiology","volume":" ","pages":"1887-1900"},"PeriodicalIF":1.9,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12350976/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144246393","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Isolation and molecular characterization of Mycoplasma ovipneumoniae associated with respiratory infection in sheep and goats in South India. 南印度绵羊和山羊呼吸道感染相关卵肺炎支原体的分离和分子特征

IF 1.9 4区生物学

Brazilian Journal of Microbiology Pub Date : 2025-09-01 Epub Date: 2025-04-26 DOI: 10.1007/s42770-025-01663-4

Shanmugasundaram Udhayavel, Kuppannan Sukumar, Kuppusamy Senthilkumar, Palani Srinivasan, Ayyasamy Elango

{"title":"Isolation and molecular characterization of Mycoplasma ovipneumoniae associated with respiratory infection in sheep and goats in South India.","authors":"Shanmugasundaram Udhayavel, Kuppannan Sukumar, Kuppusamy Senthilkumar, Palani Srinivasan, Ayyasamy Elango","doi":"10.1007/s42770-025-01663-4","DOIUrl":"10.1007/s42770-025-01663-4","url":null,"abstract":"Mycoplasma ovipneumoniae is a significant pathogen causing atypical or non-progressive pneumonia in small ruminants, leading to considerable economic losses in the global small-ruminant industry. In this study, M. ovipneumoniae was isolated from sheep and goats showing respiratory symptoms in Tamil Nadu, India. A total of 200 samples, consisting of 186 nasal swabs and 14 lung tissue samples, were collected from animals exhibiting characteristic respiratory signs. The samples were cultured in Mycoplasma experience liquid media, and a color change indicated growth of mycoplasma, which was subsequently analyzed using a PCR assay targeting the 16 S rRNA gene specific to M. ovipneumoniae. Out of the 200 samples, 60 (30%) tested positive for M. ovipneumoniae in the PCR test. The broth cultures positive by PCR were further subjected to isolation on Mycoplasma Experience solid medium. Among the 60 PCR-positive cultures, 45 displayed typical \"fried egg\" micro-colonies, which were confirmed as M. ovipneumoniae through PCR. 16 S rRNA gene sequencing was conducted on seven of these isolates. The sequences were submitted to GenBank with accession numbers (PQ013702 to PQ013708). Phylogenetic analysis revealed that the seven isolates were closely related, forming two main clusters and grouping with Indian strains from the states of Kerala and Karnataka. This study highlights the prevalence of M. ovipneumoniae in small ruminants in Tamil Nadu and its genetic similarity with strains from neighboring regions. Molecular characterization of local isolates of M. ovipneumoniae could form the basis for development of rapid pen side diagnostic kit and vaccine for M. ovipneumoniae.","PeriodicalId":9090,"journal":{"name":"Brazilian Journal of Microbiology","volume":" ","pages":"2087-2095"},"PeriodicalIF":1.9,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12350858/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143969160","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Lactobacilli and gut pH: features of correlation in model experiments. 乳酸杆菌与肠道pH：模型实验的相关性特征。

IF 1.9 4区生物学

Brazilian Journal of Microbiology Pub Date : 2025-09-01 Epub Date: 2025-07-10 DOI: 10.1007/s42770-025-01734-6

Maria S Mazanko, Elena V Mazanko, Sergey A Emelyantsev, Michael L Chikindas, Dimitry V Rudoy

{"title":"Lactobacilli and gut pH: features of correlation in model experiments.","authors":"Maria S Mazanko, Elena V Mazanko, Sergey A Emelyantsev, Michael L Chikindas, Dimitry V Rudoy","doi":"10.1007/s42770-025-01734-6","DOIUrl":"10.1007/s42770-025-01734-6","url":null,"abstract":"Representatives of lactobacilli are among the most common probiotics, largely due to their prevalence as microorganisms involved in the fermentation of dairy products. However, research on their properties is often conducted under laboratory conditions, which are different from the conditions in the gut, making it difficult to extrapolate the obtained data. In our study, we demonstrate how the properties of two probiotic strains, Limosilactobacillus frumenti KL31 and Ligilactobacillus salivarius KL61, change under model conditions that approximate those of the gut, and we explore the relationship between lactic acid bacteria and the pH levels of the intestinal environment. Under gut-simulating conditions, lactobacilli produce less lactic acid and exhibit antioxidant and DNA-protective properties to a lesser extent than in laboratory conditions. The addition of simple or complex sugars stimulates a decrease in the pH of the model cecal contents. In turn, the pH level itself affects the growth of lactobacilli. The growth rate of Limosilactobacillus frumenti KL31 decreases when the pH of the medium increases above 7.0 since this microorganism prefers a lower pH. In contrast, Enterococcus spp. prefer a more alkaline pH, while the growth of Escherichia coli is largely independent of the environment's acidity. Due to the difficulty of assessing the probiotic properties of lactobacilli under standard laboratory conditions and the strong dependence of their growth and activity on intestinal conditions, it is necessary to use media and conditions that simulate the intestinal environment of the potential host organism when screening potential probiotic strains.","PeriodicalId":9090,"journal":{"name":"Brazilian Journal of Microbiology","volume":" ","pages":"1471-1481"},"PeriodicalIF":1.9,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12350998/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144599417","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enhanced antibacterial and anti-inflammatory properties of Hwangryeonhaedok-tang fermented with Kimchi-derived Lactiplantibacillus plantarum WiKim0111 for potential acne treatment. 泡菜来源的植物乳酸杆菌WiKim0111发酵的黄莲海德汤具有增强的抗菌和抗炎特性，可用于治疗痤疮。

IF 1.9 4区生物学

Brazilian Journal of Microbiology Pub Date : 2025-09-01 Epub Date: 2025-06-23 DOI: 10.1007/s42770-025-01703-z

Ho Jae Lee, Chang Hee Jeong, Tae-Woon Kim, Sang Wan Seo, Do-Won Jeong, Sung Wook Hong

{"title":"Enhanced antibacterial and anti-inflammatory properties of Hwangryeonhaedok-tang fermented with Kimchi-derived Lactiplantibacillus plantarum WiKim0111 for potential acne treatment.","authors":"Ho Jae Lee, Chang Hee Jeong, Tae-Woon Kim, Sang Wan Seo, Do-Won Jeong, Sung Wook Hong","doi":"10.1007/s42770-025-01703-z","DOIUrl":"10.1007/s42770-025-01703-z","url":null,"abstract":"Recent years have seen an increase in the incidence of acne in adults and teenagers resulting in active research in acne treatment. In the present study, we aimed to improve the anti-acne effects of Hwangryeonhaedok-tang (HHT) through fermentation using a kimchi-derived lactic acid bacterium. Lactiplantibacillus plantarum WiKim0111 (Lpb. plantarum WiKim0111) was selected to ferment HHT due to its strong antioxidant activity and its ability to effectively survive and grow in the HHT medium. The antimicrobial activity of HHT and fermented HHT (FHHT) against Cutibacterium acnes (C. acnes) was determined using an agar well diffusion assay. FHHT exhibited stronger antimicrobial, radical scavenging, and superoxide dismutase activity compared to HHT. RAW 264.7 cells pretreated HHT or FHHT showed significantly lower production of nitric oxide, interleukin (IL) -1β, and IL-6 than lipopolysaccharide -treated cells, with FHHT demonstrating higher efficiency than HHT. Overall, HHT fermented by Lpb. plantarum WiKim0111 displayed enhanced antioxidant and anti-inflammatory properties, as well as greater antibacterial activity against C. acnes compared to unfermented HHT. These findings suggest that FHHT may help mitigate acne symptoms by reducing oxidative stress, modulating inflammation, and directly inhibiting the growth of C. acnes. Further in vitro studies focusing on sebum-producing skin cells, as well as clinical trials evaluating the topical or oral application of FHHT, would be valuable to confirm its efficacy and safety in acne treatment.","PeriodicalId":9090,"journal":{"name":"Brazilian Journal of Microbiology","volume":" ","pages":"1459-1469"},"PeriodicalIF":1.9,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12350970/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144474017","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mycoplasma haemofelis and 'Candidatus Mycoplasma haemominutum' in domestic cats from Pelotas, RS: molecular detection and risk factors. 来自RS Pelotas家猫的血支原体和候选血支原体：分子检测和危险因素。

IF 1.9 4区生物学

Brazilian Journal of Microbiology Pub Date : 2025-09-01 Epub Date: 2025-06-23 DOI: 10.1007/s42770-025-01712-y

Paola Renata Joanol Dallmann, Diago Dutra Lima, Alexsander Ferraz, Taise Vencato Isquierdo, Ceres Cristina Tempel Nakasu, Charles Silva de Lima, William Borges Domingues, Leandro Silva Nunes, Antonio Duarte Pagano, Vinicius Farias Campos, Kauê Rodrigues Martins, Rodrigo Casquero Cunha

{"title":"Mycoplasma haemofelis and 'Candidatus Mycoplasma haemominutum' in domestic cats from Pelotas, RS: molecular detection and risk factors.","authors":"Paola Renata Joanol Dallmann, Diago Dutra Lima, Alexsander Ferraz, Taise Vencato Isquierdo, Ceres Cristina Tempel Nakasu, Charles Silva de Lima, William Borges Domingues, Leandro Silva Nunes, Antonio Duarte Pagano, Vinicius Farias Campos, Kauê Rodrigues Martins, Rodrigo Casquero Cunha","doi":"10.1007/s42770-025-01712-y","DOIUrl":"10.1007/s42770-025-01712-y","url":null,"abstract":"Hemotropic mycoplasmas are small, pleomorphic, gram-negative, epierithrocytic bacteria that infect a wide variety of animals, including domestic cats. These microorganisms cause the disease known as feline hemotropic mycoplasmosis (MHF), also called feline infectious anemia. The objective of the study was to use PCR assays based on amplification of 16S rDNA to detect the species Mycoplasma haemofelis (Mhf) and 'Candidatus Mycoplasma haemominutum' (CMhm) in felines treated at a clinic specialized exclusively in cats in the city of Pelotas, Rio Grande do Sul. Furthermore, the research aimed to identify potential risk factors associated with the infection. A total of 253 blood samples from domestic felines were analyzed, of which 76 samples (30%) were positive for at least one species. The most detected hemoplasma was CMhm, with 51/253 (20.1%), followed by Mhf, with 23/253 (9.1%). Mhf and CMhm co-infection was detected in 2/253 (0.8%) samples. Felines with access to the street, presence of ectoparasites, co-infection with FeLV, FIV and other diseases (unhealthy animals) were more likely to be infected by hemoplasmas.","PeriodicalId":9090,"journal":{"name":"Brazilian Journal of Microbiology","volume":" ","pages":"2163-2168"},"PeriodicalIF":1.9,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12350854/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144474018","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biological synthesis of gold nanoparticles using endophytic fungus Cladosporium sphaerospermum (RUV07) extract: characterization and in vitro antiproliferative effect of A549 cells. 内生真菌球藻枝孢（Cladosporium sphaerospermum, RUV07）提取物生物合成纳米金：表征及体外抗A549细胞增殖作用

IF 1.9 4区生物学

Brazilian Journal of Microbiology Pub Date : 2025-09-01 Epub Date: 2025-04-26 DOI: 10.1007/s42770-025-01675-0

Surendirakumar Kannaiah, Prabhu Raju, Narmatha Sekar, Bandana Sanasam, Thokchom Nepolian Singh, Nongthombam Kistu Singh, Sathish Muthukrishnan

{"title":"Biological synthesis of gold nanoparticles using endophytic fungus Cladosporium sphaerospermum (RUV07) extract: characterization and in vitro antiproliferative effect of A549 cells.","authors":"Surendirakumar Kannaiah, Prabhu Raju, Narmatha Sekar, Bandana Sanasam, Thokchom Nepolian Singh, Nongthombam Kistu Singh, Sathish Muthukrishnan","doi":"10.1007/s42770-025-01675-0","DOIUrl":"10.1007/s42770-025-01675-0","url":null,"abstract":"In this groundbreaking research, we utilized the fungal extract of Cladosporium sphaerospermum -RVU07 as a powerful reducing agent to create bioactive gold nanoparticles (Au-NPs). The comprehensive material characterization of the Au-NPs was conducted using methods including XRD, SEM, TEM, UV-vis, and DLS analysis. Notably, the successful formation of Au-NPs was confirmed by a striking color change of the precursor from pale yellow to vibrant violet. This transformation underscores the pivotal role of fungal bioactive molecules, which impart distinctive physicochemical and biological properties to the Au-NPs. The extraction of these compounds significantly influenced both the particle size and surface morphology, enhancing their effectiveness. We investigated the antiproliferative effect of the bioactive Au-NPs against lung cancer cells specifically A549. Our findings revealed that these nanoparticles and bioactive molecules effectively induce ROS production and drive nuclear damage-mediated cell death. With their nanoscale dimensions, the Au-NPs rapidly penetrate the atomic region of A549 cancer cells, facilitating substantial cellular dysfunction. Our nuclear damage assays confirmed that the Au-NPs lead to pronounced nuclear fragmentation and shrinkage in A549 cells following treatment. Additionally, we evaluated the biocompatibility of the Au-NPs against PBMC cells. The biosafety assay results convincingly showed that the bioactive molecules on the Au-NPs' surface significantly mitigate their toxic effects on normal cells. This research highlights the immense potential of mycosynthesized bioactive Au-NPs as innovative pharmaceuticals for a wide array of cancer therapies and biomedical applications, heralding a new era in targeted treatment solutions.","PeriodicalId":9090,"journal":{"name":"Brazilian Journal of Microbiology","volume":" ","pages":"1447-1457"},"PeriodicalIF":1.9,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12350880/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143976696","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Antimicrobial resistance and genetic diversity of Escherichia coli isolated from marine bivalves. 海洋双壳类分离的大肠埃希菌的耐药性和遗传多样性。

IF 1.9 4区生物学

Brazilian Journal of Microbiology Pub Date : 2025-09-01 Epub Date: 2025-05-22 DOI: 10.1007/s42770-025-01696-9

Jailton Lobo da Costa Lima, Marcelly Miranda Aybal Jayme, Felipe Piedade Gonçalves Neves, Angela Corrêa de Freitas-Almeida, Mara Lúcia Penna Queiroz, Fabio Vieira de Araujo

{"title":"Antimicrobial resistance and genetic diversity of Escherichia coli isolated from marine bivalves.","authors":"Jailton Lobo da Costa Lima, Marcelly Miranda Aybal Jayme, Felipe Piedade Gonçalves Neves, Angela Corrêa de Freitas-Almeida, Mara Lúcia Penna Queiroz, Fabio Vieira de Araujo","doi":"10.1007/s42770-025-01696-9","DOIUrl":"10.1007/s42770-025-01696-9","url":null,"abstract":"Escherichia coli can contaminate the marine environment through sewage and accumulate in bivalve molluscs. We assessed antimicrobial resistance (AMR) mechanisms and genetic diversity of 69 E. coli isolates recovered from in natura, boiled, and purchased Perna perna mussels in Niterói city, Brazil. All isolates were sensitive to cefoxitin, imipenem, meropenem, and fosfomycin. In contrast, 61 (88.4%) isolates were not susceptible to streptomycin. We found ten (14.5%) multidrug-resistant (MDR) isolates. Extended-spectrum beta-lactamase (ESBL) production was detected in 30 (43.5%) isolates, with 14 (46.7%) isolates carrying ESBL genes (blaTEM, blaCTX-M9, blaSHV, blaCTX-M2, and blaCTX-M8/25). Quinolone/fluoroquinolone resistance was observed in 12 (17.4%) isolates, with qnrS and qnrB genes identified. Nine (13%) isolates were resistant to folate pathway inhibitors, with sul1 and sul2 genes identified. Only one (1.5%) isolate had the intI integrase gene. Most isolates had the A/C replicon (73.9%) and belonged to phylogenetic group B1 (50.7%). PFGE analysis revealed the existence of 54 genotypes, with four clusters containing isolates from different sources. The presence of ESBL-producing strains, AMR genes, and diverse plasmid replicons highlights the role of marine environments in AMR dissemination. Continuous AMR surveillance in marine ecosystems is crucial to mitigate public health risks linked to seafood consumption.","PeriodicalId":9090,"journal":{"name":"Brazilian Journal of Microbiology","volume":" ","pages":"1585-1593"},"PeriodicalIF":1.9,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12350904/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144118865","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0