Brazilian Journal of Microbiology最新文献

{"title":"development and in vitro characterization of a Trichoderma-based biopesticide for the management of Fusarium solani in tomato.","authors":"Thulfiqear A Jabbar, Aqeel N Al-Abedy, AbdulZahra J Ali","doi":"10.1007/s42770-026-02062-z","DOIUrl":"https://doi.org/10.1007/s42770-026-02062-z","url":null,"abstract":"<p><p>| The aim of this research was to create a sustainable biopesticide based on Trichoderma intended for use against the pathogen Fusarium solani in tomato. Individual and collective antagonistic potential of six Trichoderma spp. isolates was assessed through in vitro dual culture assays. Individual isolates showed inhibitory rates of 68.2% to 87.4%, while the highest increase in suppressive efficacy to 94.5% was obtained with a synergistic consortium of all six isolates. Based on simple effects analysis, the ideal range for maximum physiological vigor was a 32-45% increase in growth at temperatures of 25-30°C, pH 6, and salinity of 5 dS m⁻¹. Additionally, the presence of 0.75 g L⁻¹ of chelated Fe, Mn, and Zn supplemented to the growth medium increased radial growth and biomass by 28% and 36%, respectively. For the purpose of large-scale production, Ziziphus spina-christi leaf powder was the best substrate, producing the highest propagule density of [Formula: see text] CFU mL⁻¹, which exceeded the productivity of conventional media by 22-40%. A composite formulation of a carrier blend (vermiculite, ground palm fronds, sand, and clay) showed satisfactory biological stability and [Formula: see text] CFU mL⁻¹ biological activity for 360 days of storage at refrigeration temperatures, with more than 75% of its viability maintained. Furthermore, time-series regression analysis confirmed that the temporal loss of fungal viability followed a highly predictable model ([Formula: see text]), while carrier-specific regression modeling demonstrated that the optimized 'mixture' carrier significantly decelerated the decline kinetics compared to the non-formulated control. The results show that the formulated consortium offers an innovative, stable, high quality, and environmentally friendly substitute to chemical fungicides for use in Integrated Disease Management (IDM) programs.</p>","PeriodicalId":9090,"journal":{"name":"Brazilian Journal of Microbiology","volume":"57 1","pages":""},"PeriodicalIF":2.1,"publicationDate":"2026-08-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13486428/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148787765","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biotechnological potential of Paenarthrobacter nicotinovorans ATCC 49919 for the microbial conversion of nicotine and tobacco extracts into 6-hydroxynicotine.","authors":"Iustin-Tiberius Munteanu, Ana Huşleag, Robert Iustin Susanu, Andreea-Mihaela Mleşniță, Marius Mihăşan","doi":"10.1007/s42770-026-02064-x","DOIUrl":"https://doi.org/10.1007/s42770-026-02064-x","url":null,"abstract":"<p><p>Nicotine-rich wastes from the tobacco industry represent an environmental liability but also a potential feedstock for value-added bioconversion. In this study, the biotechnological potential of Paenarthrobacter nicotinovorans ATCC 49919 for selective accumulation of 6-hydroxy-(L)-nicotine (6HLN), the first intermediate of the pyridine pathway, was evaluated under flask and bioreactor conditions. Two metabolic engineering strategies were explored: transcriptional silencing of the 6-hydroxynicotine oxidase gene (6hlnO) using a CRISPR/dCas9 system and overexpression of the nicotine dehydrogenase large subunit (ndhL) to enhance entry flux into the pathway. Among the tested strains, the ndhL-overexpressing derivative exhibited the highest 6HLN accumulation during growth (up to 1.78 g L⁻¹; space-time yield 104.4 mg L⁻¹ h⁻¹). Resting-cell bioconversion experiments identified 100 g wet cell weight L⁻¹ as an optimal biomass loading. Chemical inhibition of 6-hydroxy-L-nicotine oxidase with 0.8 mM ZnSO₄ further expanded the 6HLN accumulation window without substantially impairing nicotine depletion. Scale-up experiments in a stirred bioreactor demonstrated effective conversion of both pure nicotine and tobacco-derived extracts, with matrix-dependent differences in conversion kinetics. Dissolved oxygen profiles correlated reproducibly with substrate depletion and product turnover, providing a potential online process control signature. Although not achieving near-stoichiometric yields reported for knockout-based systems, the described platform offers a controllable and feedstock-flexible approach for nicotine waste valorization.</p>","PeriodicalId":9090,"journal":{"name":"Brazilian Journal of Microbiology","volume":"57 1","pages":""},"PeriodicalIF":2.1,"publicationDate":"2026-08-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13486417/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148788074","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genetic architecture and functional dynamics of integrons in Staphylococcus aureus resistance.","authors":"Anjaneyulu Musini, Madeeha Owais, Rerelly Joshika","doi":"10.1007/s42770-026-02019-2","DOIUrl":"https://doi.org/10.1007/s42770-026-02019-2","url":null,"abstract":"<p><p>Staphylococcus aureus is a major opportunistic pathogen and a leading cause of community and hospital-acquired infections worldwide, with a growing capacity to develop multidrug resistance (MDR). Among the genetic mechanisms driving this resistance, integrons play a critical role by capturing, rearranging, and expressing antimicrobial resistance gene cassettes through site-specific recombination. Although integrons are traditionally associated with Gram-negative bacteria, increasing evidence highlights their significant contribution to resistance dissemination in S. aureus, particularly in methicillin-resistant S. aureus (MRSA) strains. This review focuses on current knowledge on the structure, function, and types of integrons, and their role in prevalence and mechanistic role in S. aureus. Integrons interact with other mobile genetic elements like transposons and plasmids to enable horizontal gene transfer across strains and species. Among the four recognized classes of integrons, class 1 is the most prevalent in S. aureus frequently associated with plasmids and transposons and show resistance to β-lactams, aminoglycosides, fluoroquinolones, and tetracyclines. Class 2 integrons occur less commonly and exhibit limited cassette diversity, while class 3 integrons remain rare. Epidemiological studies report class 1 integron prevalence ranging from 40 to 70% in clinical isolates across different regions, often correlating with resistance to β-lactams, aminoglycosides, fluoroquinolones, tetracyclines, and rifampicin, and co-occurring with SCCmec elements in MRSA. Integron-mediated resistance is further enhanced by stress-induced integrase activity and biofilm formation, promoting persistence in healthcare settings. Molecular detection methods, particularly multiplex PCR targeting integrase genes, are essential for surveillance. Understanding integron dynamics in S. aureus is crucial for informing antimicrobial stewardship, infection control strategies, and future interventions aimed at limiting the spread of MDR pathogens.</p>","PeriodicalId":9090,"journal":{"name":"Brazilian Journal of Microbiology","volume":"57 1","pages":""},"PeriodicalIF":2.1,"publicationDate":"2026-08-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13486446/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148787853","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An experimental multiplex PCR/RT-PCR for detection of the main viruses associated with equine neurological disease.","authors":"S O Scherer, J V J Silva Júnior, N H Pedroso, I F Dionisio, R Weiblen, E F Flores","doi":"10.1007/s42770-026-02065-w","DOIUrl":"10.1007/s42770-026-02065-w","url":null,"abstract":"<p><p>Viral neurological infections are important causes of morbidity and mortality in horses worldwide. Herein, we describe an end-point multiplex PCR/RT-PCR for simultaneous and differential detection of major equine encephalitis-related agents: rabies virus (RABV), equine herpesvirus type 1 (EHV-1), alphaviruses [Venezuelan equine encephalitis virus (VEEV), Eastern equine encephalitis virus (EEEV), Western equine encephalitis virus (WEEV) and Madariaga virus (MADV)] and flaviviruses [West Nile virus (WNV), Saint Louis encephalitis virus (SLEV), Rocio virus (ROCV) and Japanese encephalitis virus (JEV)]. The multiplex assay successfully amplified all targets either individually or simultaneously: alphavirus (381 bp-experimentally evaluated for MADV and potentially applicable to EEEV and WEEV based on primer design and sequence conservation), EHV-1 (255 bp), RABV (173 bp), VEEV (119 bp), and flavivirus (97-101 bp-experimentally evaluated using ROCV, with the potential to also detect WNV, SLEV and JEV, based on primer design and sequence conservation). Furthermore, the assay demonstrated analytical sensitivity and specificity that support its use for the comprehensive etiological diagnosis of equine neurological diseases. Overall, the multiplex PCR/RT-PCR described here may contribute to the diagnosis of viral neurological infections in horses, reducing costs and turnaround time, while contributing to prevention and control measures.</p>","PeriodicalId":9090,"journal":{"name":"Brazilian Journal of Microbiology","volume":"57 1","pages":""},"PeriodicalIF":2.1,"publicationDate":"2026-08-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13486407/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148788072","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Performance evaluation of methods for detecting enteric viruses in meat products.","authors":"Lucas Zanchetta, Paula Rogovski, Henrique Borges da Silva Grisard, Beatriz Pereira Savi, Catielen Paula Pavi, Sabrina Carvalho Suominsky, Giulia Von Tönnemann Pilati, Gislaine Fongaro","doi":"10.1007/s42770-026-02058-9","DOIUrl":"https://doi.org/10.1007/s42770-026-02058-9","url":null,"abstract":"<p><p>Meat products represent important vehicles for foodborne viral transmission, yet standardized detection methodologies for these complex matrices remain limited. This study systematically compared three viral recovery methods across nine meat products representing chicken, pork, and processed deli meat matrices: polyethylene glycol 6000 (PEG) precipitation, glycine buffer-chloroform (GBC) phase separation, and β-mercaptoethanol (B-M) chaotropic lysis. Using Murine Norovirus 1 (MNV-1) and Murine Hepatitis Virus 3 (MHV-3) as process controls, recovery efficiency was assessed by RT-qPCR. The B-M method demonstrated the most consistent performance, successfully detecting MNV-1 across all tested matrices and significantly outperforming PEG and GBC for MNV-1 in chicken and pork. Applying the B-M method to 25 commercial retail products revealed the genetic material of Hepatitis E virus (HEV) in four samples and Porcine Circovirus type 2 (PCV-2) in one sample, exclusively within pork products. These findings provide critical comparative performance data for method selection in viral surveillance of meat and document the occurrence of HEV and PCV-2 genetic material in commercial pork products from Santa Catarina, Brazil, highlighting a significant food and agricultural biosafety concern in this major meat-producing and exporting state.</p>","PeriodicalId":9090,"journal":{"name":"Brazilian Journal of Microbiology","volume":"57 1","pages":""},"PeriodicalIF":2.1,"publicationDate":"2026-08-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13486440/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148788128","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterization of 2-deoxyribose-5-phosphate aldolase from Bacillus altitudinis JYY-02.","authors":"Wentao Feng, Chenglei Qin, Wenjun Deng, Qunqun Guo, Chao Wang, Guicai Du, Ronggui Li","doi":"10.1007/s42770-026-02055-y","DOIUrl":"https://doi.org/10.1007/s42770-026-02055-y","url":null,"abstract":"<p><p>HMG-CoA reductase inhibitors, commonly known as statins, effectively reduce low-density lipoprotein cholesterol levels in the blood, thereby playing a key role in the treatment of cardiovascular and cerebrovascular diseases. The biosynthesis of the compounds can be achieved via deoxyriboaldolase. In this study, a 672-bp gene coding 2-deoxyribose-5-phosphate aldolase (DERA) was identified from the genome of Bacillus altitudinis JYY-02 and overexpressed in Escherichia coli BL21(DE3). The recombinant DERA exhibited a molecular weight of 23.15 kDa and was purified by Ni-NTA affinity chromatography with a 12.9-fold increase in purity and a final yield of 51.2%. Molecular docking analysis revealed that Lys, Leu, Thr, Ser, and Gly are the key amino acid residues involved in the interaction between DERA and its substrate, 2-deoxyribose-5-phosphate. The effects of pH, temperature, and metal ions on enzyme activity were investigated. The optimal pH and temperature for activity were pH7.0 and 45 °C, respectively. Using 2-deoxyribose-5-phosphate as the substrate, the Vmax and the Km values were calculated as 6.978 µmol/mg·pr/min and 8.398 mM, respectively. Thermal stability assays showed that the enzyme retained 79% and 39% of its initial activity after 1 h of incubation at 60 °C and 90 °C respectively. Bioassay of acetaldehyde tolerance of DERA revealed that DERA retained 77.5%, 55.6%, and 34.7% activity after treatment with 100, 200 and 300 mM acetaldehyde for 2 h, respectively. Furthermore, using acetaldehyde and chloroacetaldehyde as substrates, DERA catalyzed the formation of S-4-chloro-3-hydroxybutyraldehyde (S-CHBAL), indicating its potential for application in the biosynthesis of statin intermediates.</p>","PeriodicalId":9090,"journal":{"name":"Brazilian Journal of Microbiology","volume":"57 1","pages":""},"PeriodicalIF":2.1,"publicationDate":"2026-08-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13473057/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148757394","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Simultaneous nitrogen removal and diclofenac degradation by Alcaligenes sp. FDN-09 under carbon-limited conditions.","authors":"Chuangchuang Yin, Zhiqi Lu, Hongjing Li, Jiong Du, Weibin Bao","doi":"10.1007/s42770-026-02056-x","DOIUrl":"10.1007/s42770-026-02056-x","url":null,"abstract":"<p><p>Microorganisms capable of simultaneously achieving nitrogen removal and emerging organic contaminants (EOCs) degradation under carbon-limited conditions are highly desirable for wastewater treatment, however, such multifunctional performance remains poorly understood. Diclofenac (DCF), a frequently detected pharmaceutical with recognized environmental risks, is particularly recalcitrant to biological removal. In this study, an Alcaligenes sp. FDN-09 was identified as a strain capable of concurrent ammonium nitrogen removal and DCF attenuation under low C/N conditions. Optimal performance was achieved at C/N ratio of 4.0, with total nitrogen and DCF removal efficiencies of 63.3% and 80.2%, respectively, without detectable accumulation of NO₂⁻-N, NO₃⁻-N, or N₂O. Nitrogen balance analysis combined with genomic evidence revealed the presence of the dnfA/B/C gene cluster and the absence of conventional ammonia monooxygenase and hydroxylamine oxidoreductase genes, suggesting the involvement of alternative nitrogen transformation routes, including Dirammox-like pathways. Functional gene annotation and liquid chromatography-mass spectrometry analysis further indicated that DCF attenuation by strain FDN-09 may proceed through multiple transformation reactions, including hydroxylation, dechlorination, C-N bond cleavage, and aromatic ring opening, leading to the identification of nine major transformation products. Overall, this study provides process-level and genomic insights into the potential of Alcaligenes sp. FDN-09 for simultaneous nitrogen transformation and DCF degradation under carbon-limited conditions, supporting the feasibility of integrated nitrogen and EOCs removal in biological wastewater treatment systems.</p>","PeriodicalId":9090,"journal":{"name":"Brazilian Journal of Microbiology","volume":"57 1","pages":""},"PeriodicalIF":2.1,"publicationDate":"2026-08-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13469058/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148720762","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Production optimization scale-up and application of a multifunctional enzyme cocktail for eco-friendly pulp biobleaching.","authors":"Steffy Angural, Sunena Jassal, Anupama Sharma, Kumud Sharma, Naveen Gupta","doi":"10.1007/s42770-026-02051-2","DOIUrl":"10.1007/s42770-026-02051-2","url":null,"abstract":"<p><p>In the paper industry, pulp bleaching is an essential step to increase the whiteness and brightness of paper, which is typically achieved using chlorine-based or oxidative chemicals that cause significant environmental pollution. In the present study, Bacillus tequilensis LXM 55 produced a cocktail of lignocellulolytic and hemicellulolytic enzymes. Solid-state fermentation (SSF) is a cost-effective and efficient method suitable for large-scale enzyme production. Therefore, the SSF process was optimized for the production of the enzyme cocktail employing traditional and statistical methods. Optimization resulted in a significant improvement in enzyme yields, with 34.0-, 22.85-, and 20.37-fold increases in enzyme yield for laccase (L) (4518.54 IU g⁻¹), xylanase (X) (2664.81 IU g⁻¹), and mannanase (M) (1448.42 IU g⁻¹), respectively. For commercial viability, the optimized process was successfully scaled up to a 1 kg substrate level without any significant loss in enzyme productivity. The enzyme cocktail (L+X+M) was applied to pulp bleaching, resulting in a 48% reduction in kappa number and a 12.1% improvement in brightness. Most significantly, the enzymatic pre-treatment of pulp led to a 40% reduction in chlorine usage while achieving the same brightness and whiteness as pulp treated with the traditional chemical-based process. These findings demonstrate the potential of the enzyme cocktail as an efficient, eco-friendly, and industrially scalable alternative for sustainable pulp bleaching in the paper industry.</p>","PeriodicalId":9090,"journal":{"name":"Brazilian Journal of Microbiology","volume":"57 1","pages":""},"PeriodicalIF":2.1,"publicationDate":"2026-08-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13462030/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148705361","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"From decay to discovery: A new antimicrobial peptide from Chrysomya megacephala (Diptera: Calliphoridae) larvae.","authors":"Nurul Azmiera, Hassanain Al-Talib, Anna Krasilnikova, Shariza Sahudin, Noraziah Sahlan, Chong Chin Heo","doi":"10.1007/s42770-026-02047-y","DOIUrl":"10.1007/s42770-026-02047-y","url":null,"abstract":"<p><p>The larvae of Chrysomya megacephala (Diptera: Calliphoridae) thrive in environments rich in decaying organic matter and dead animals, which are often colonized by bacteria. This adaptation suggests the larvae possess antimicrobial substances that provide them with a defense against bacterial infection. The hemolymph from around 200 C. megacephala larvae previously exposed to methicillin-resistant Staphylococcus aureus (MRSA) (clinical isolate) was collected, purified, and isolated utilizing Reverse Phase High Performance Liquid Chromatography (RP-HPLC). Fractions exhibiting antimicrobial properties underwent further analysis using Quadrupole Time-of-Flight Liquid Chromatography Mass Spectrometry (QTOF-LCMS). The resulting mass spectra were translated into amino acid sequences through de novo algorithms. Antimicrobial prediction tools from repositories such as APD3, Database of Antimicrobial Activity and Structure of Peptides (DBSAAP), Database of Antimicrobial Peptide (DBAMP), and Collection of Antimicrobial Peptide Version 4 (CAMPR4) were employed to validate the peptide sequences. We found the peptide (HGCGRLSKWFRQPGLLLSVKR) exhibited partial similarity with C. megacephala's heat shock protein 70 (hsp70). The peptide demonstrated activity against Staphylococcus aureus, including MRSA, Micrococcus luteus, Staphylococcus epidermidis, and Bacillus subtilis with a minimum inhibitory concentration (MIC) of 0.06 mg/ml and an inhibition zone ranging from 8 to 11 mm at a concentration of 1 mg/ml. The cytotoxicity test of the peptide on immortalized keratinocyte cells (HaCat) and human corneal epithelial cells (HCEC) revealed an average cell viability > 80% at concentration of 0.06 mg/ml. In conclusion, the novel peptide (HGCGRLSKWFRQPGLLLSVKR) exhibits a broad spectrum of activity against Gram-positive bacteria while demonstrating minimal toxicity towards human cells.</p>","PeriodicalId":9090,"journal":{"name":"Brazilian Journal of Microbiology","volume":"57 1","pages":""},"PeriodicalIF":2.1,"publicationDate":"2026-08-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13457817/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148700765","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of lactic acid bacterial starter Pediococcus acidilactici KUB-M6 on corn stover silage quality: microbial and biochemical properties.","authors":"Kritsada Phetduang, Luu Tang Phuc Khang, Niphawan Panti, Nguyen Vu Linh, Massalin Nakphaichit, Siriwat Jinsiriwanit, Sunee Nitisinprasert, Phatthanaphong Therdtatha","doi":"10.1007/s42770-026-02042-3","DOIUrl":"10.1007/s42770-026-02042-3","url":null,"abstract":"<p><p>Corn stover is an abundant agricultural residue that can be converted into a stable feed resource through ensiling, yet the combined effects of moisture content and microbial inoculation on fermentation dynamics remain poorly resolved. Here, we investigated the temporal effects of Pediococcus acidilactici KUB-M6 inoculation under three moisture regimes during 21 days of corn stover ensiling. KUB-M6 substantially altered the physicochemical, organic acid and microbial profiles of the silage. Inoculated silages accumulated significantly more lactic acid and total organic acids than uninoculated controls, with the highest concentrations observed at 70% moisture on day 14 (9.35% DM and 13.50% DM, respectively). Acetic acid also reached its maximum concentration under this condition (4.15% DM), whereas propionic and butyric acids were not detected in KUB-M6-inoculated silages. Inoculation was accompanied by higher total bacterial and lactic acid bacterial populations and lower yeast and Enterobacteriaceae abundances, particularly under higher-moisture conditions. Multivariate analyses further separated inoculated and control silages and revealed coordinated associations among organic acid accumulation, physicochemical properties and microbial populations. These findings demonstrate that Pd. acidilactici KUB-M6 substantially reshaped the fermentation trajectory of corn stover, with 70% moisture associated with the greatest accumulation of lactic and total organic acids during ensiling.</p>","PeriodicalId":9090,"journal":{"name":"Brazilian Journal of Microbiology","volume":"57 1","pages":""},"PeriodicalIF":2.1,"publicationDate":"2026-08-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13457520/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148700722","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}