Brazilian Journal of Microbiology最新文献_第8页

Antimicrobial and antibiofilm activities of Brazilian organic honey against oral microorganisms. 巴西有机蜂蜜对口腔微生物的抗菌和抗生物膜活性。

IF 2.1 4区生物学

Brazilian Journal of Microbiology Pub Date : 2024-09-01 Epub Date: 2024-05-15 DOI: 10.1007/s42770-024-01343-9

Diego Romário-Silva, Marcelo Franchin, Severino Matias Alencar, Bruno Bueno-Silva, Janaína de Cássia Orlandi Sardi, Andréa Cristina Barbosa da Silva, Fabiane Cruz-Vieira, Priscila Vieira da Silva, Pedro Luiz Rosalen

{"title":"Antimicrobial and antibiofilm activities of Brazilian organic honey against oral microorganisms.","authors":"Diego Romário-Silva, Marcelo Franchin, Severino Matias Alencar, Bruno Bueno-Silva, Janaína de Cássia Orlandi Sardi, Andréa Cristina Barbosa da Silva, Fabiane Cruz-Vieira, Priscila Vieira da Silva, Pedro Luiz Rosalen","doi":"10.1007/s42770-024-01343-9","DOIUrl":"10.1007/s42770-024-01343-9","url":null,"abstract":"Objective: To evaluate the antimicrobial activity of Brazilian honeys against oral microorganisms.Design: Organic honeys (OH-1 to OH-8) were diluted (%-w/v) and sterilized by filtration. Antimicrobial activity was defined by determining MIC and CBM against oral Streptococcus. The component responsible for the antimicrobial action was defined by a catalase assay. Antibiofilm activity was evaluated against the monospecies biofilm of Streptococcus mutans (ATCC 700610).Results: OHs showed antimicrobial activity principally OH-1, OH-2, OH-3, and OH-7 with MIC values ranging between 10 and 25%. The mechanism of action occurs mainly by hydrogen peroxide produced by honey enzymes. OH-1, OH-2, and OH-7 showed total biofilm destruction at low concentrations.Conclusion: Brazilian honeys have promising antimicrobial and antibiofilm activity with the potential to control oral microbiota.","PeriodicalId":9090,"journal":{"name":"Brazilian Journal of Microbiology","volume":" ","pages":"2285-2292"},"PeriodicalIF":2.1,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11405583/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140921317","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Heterologous expression of pediocin PA-1 in Pichia pastoris: cloning, expression, characterization, and application in pork bologna preservation. 在 Pichia pastoris 中异源表达 pediocin PA-1：克隆、表达、表征及在猪肉腊肠保存中的应用。

IF 2.1 4区生物学

Brazilian Journal of Microbiology Pub Date : 2024-09-01 Epub Date: 2024-05-27 DOI: 10.1007/s42770-024-01388-w

Nguyen Pham Anh Thu, Nguyen Hieu Nghia, Dang Thi Phuong Thao, Nguyen Thi My Trinh

{"title":"Heterologous expression of pediocin PA-1 in Pichia pastoris: cloning, expression, characterization, and application in pork bologna preservation.","authors":"Nguyen Pham Anh Thu, Nguyen Hieu Nghia, Dang Thi Phuong Thao, Nguyen Thi My Trinh","doi":"10.1007/s42770-024-01388-w","DOIUrl":"10.1007/s42770-024-01388-w","url":null,"abstract":"Objective: Pediocin PA-1, an antimicrobial peptide derived from Pediococcus acidilactici PAC1.0, has a potential application as a food preservative thanks to its strong inhibitory activity against the foodborne pathogen L. monocytogenes. This study aimed to produce Pediocin PA-1 from the yeast P. pastoris and evaluate its characteristics.Methods: Gene encoding Pediocin PA-1 was integrated into P. pastoris X33 genome to establish the strain X33::ped, which could produce and secrete this peptide into culture medium. The antimicrobial activity of Pediocin PA-1 was examined using agar diffusion assay. The stability of pediocin PA-1 was determined based on its remaining antibacterial activity after exposure to proteases and extreme pH and temperatures. The potential use of this bacteriocin in food preservation was demonstrated using the L. monocytogenes infected pork bologna. The anticancer activity of Pediocin PA-1 was also investigated on some cancer cells using MTT assay.Results: We established the yeast P. pastoris X33::ped capable of producing pediocin PA-1 with antimicrobial activity against L. monocytogenes and some other harmful bacteria. Pediocin PA-1 was stable at 100˚C and resistant against pH 1-12 for 1 h, but susceptible to trypsin, α-chymotrypsin, and proteinase K. This peptide could reduce the number of L. monocytogenes in pork bologna by 3.59 log CFU/g after 7 days of storage at 4˚C. Finally, Pediocin PA-1 (25 µg/ml) inhibited the proliferation of A549 and Hela cancer cells.Conclusion: We succeeded in producing active Pediocin PA-1 from P. pastoris and demonstrated its potential use in food preservation and pharmaceutical industry.","PeriodicalId":9090,"journal":{"name":"Brazilian Journal of Microbiology","volume":" ","pages":"2169-2177"},"PeriodicalIF":2.1,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11405644/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141154083","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development and standardization of an enzyme-linked inmunosorbent for the detection of orthohantavirus infection in Argentina based on its bacterial-expressed nucleocapside protein. 基于细菌表达的核苷酸蛋白，开发用于检测阿根廷正泛病毒感染的酶联免疫吸附剂并使之标准化。

IF 2.1 4区生物学

Brazilian Journal of Microbiology Pub Date : 2024-09-01 Epub Date: 2024-06-14 DOI: 10.1007/s42770-024-01412-z

Patricia M Muzulin, Julia Brignone, Néstor Gabriel Iglesias, Marcelo Rodríguez, Lucía Irazu, Jorge B García, Carina Sen, Silvana Levis

{"title":"Development and standardization of an enzyme-linked inmunosorbent for the detection of orthohantavirus infection in Argentina based on its bacterial-expressed nucleocapside protein.","authors":"Patricia M Muzulin, Julia Brignone, Néstor Gabriel Iglesias, Marcelo Rodríguez, Lucía Irazu, Jorge B García, Carina Sen, Silvana Levis","doi":"10.1007/s42770-024-01412-z","DOIUrl":"10.1007/s42770-024-01412-z","url":null,"abstract":"We conducted a development and standardization of an IgG ELISA assay for serological detection of human orthohantavirus infections using the recombinant antigen rLECH13 produced in bacterial and derived from the LECHV. The evaluation and standardization were carried out by analyzing serum samples from a total of 50 patients with confirmed Hantavirus Pulmonary Syndrome (HPS) diagnosis through the reference technique, 50 negative sera, and 53 patients with other medical conditions. The data from the assay analysis showed a diagnostic sensitivity value of 95% and a diagnostic specificity of 80%. The high sensitivity of this novel assay leads us to conclude that rLECH13 is a feasible option for use in the immunodiagnostic of orthohantavirus infection. Additionally, it is crucial to have an antigen that can be produced under conditions that do not require highly complex laboratories. Furthermore, the new assay is cost-effective, reproducible, and demonstrates excellent performance.","PeriodicalId":9090,"journal":{"name":"Brazilian Journal of Microbiology","volume":" ","pages":"2683-2691"},"PeriodicalIF":2.1,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11405623/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141315917","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Metagenomics insight into Puga geothermal geyser located in Himalayan Geothermal Belt (Trans-Himalayan Plateau) Ladakh, India. 元基因组学对位于印度拉达克喜马拉雅地热带（跨喜马拉雅高原）的普加地热间歇泉的深入研究。

IF 2.1 4区生物学

Brazilian Journal of Microbiology Pub Date : 2024-09-01 Epub Date: 2024-06-14 DOI: 10.1007/s42770-024-01408-9

Shalini Kumari, Geetanjli Choudhary, Kumari Anu, Sarita Devi

{"title":"Metagenomics insight into Puga geothermal geyser located in Himalayan Geothermal Belt (Trans-Himalayan Plateau) Ladakh, India.","authors":"Shalini Kumari, Geetanjli Choudhary, Kumari Anu, Sarita Devi","doi":"10.1007/s42770-024-01408-9","DOIUrl":"10.1007/s42770-024-01408-9","url":null,"abstract":"Puga geothermal geyser and surrounding area, located in the Himalayan Geothermal Belt of the Trans-Himalayan Plateau in Ladakh, India, are very geographically isolated and considered pristine and free of anthropogenic activities. In this study, we have conducted the first metagenomic investigation of the microbes in and around the geyser. The whole genome sequencing analysis showed the presence of a total of 44.8%, 39.7% and 41.4% bacterial phyla in the PugW, PugS, and PugSo samples respectively, 8.6% of archaeal phyla (in all the samples), unclassified (derived from other sequences, PugW: 27.6%, PugS: 27.6%, and PugSo: 15.5%) and unclassified (derived from bacteria, PugW: 12%, PugS: 13.8%, and PugSo: 13.8%). The majority of archaeal sequences were linked to Euryarchaeota (2.84%) while the majority of the bacterial communities that predominated in most geothermal locations were linked to Pseudomonadota (67.14%) and Bacteroidota (12.52%). The abundant bacterial strains at the species level included Dechloromonas aromatica, Acinetobacter baumannii, and Arcobacter butzleri, in all the samples while the most abundant archaeal species were Methanosaeta thermophile, Methanoregula boonei, and Methanosarcina berkeri. Further, this geothermal geyser metagenome has a large number of unique sequences linked to unidentified and unclassified lineages, suggesting a potential source for novel species of microbes and their products. The present study which only examined one of the many geothermal geysers and springs in the Puga geothermal area, should be regarded as a preliminary investigation of the microbiota that live in the geothermal springs on these remote areas. These findings suggest that further investigations should be undertaken to characterize the ecosystems of the Puga geothermal area, which serve as a repository for unidentified microbial lineages.","PeriodicalId":9090,"journal":{"name":"Brazilian Journal of Microbiology","volume":" ","pages":"2321-2334"},"PeriodicalIF":2.1,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11405596/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141315954","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Utilization of Fusarium Solani lipase for enrichment of polyunsaturated Omega-3 fatty acids. 利用 Fusarium Solani 脂肪酶富集多不饱和 Omega-3 脂肪酸。

IF 2.1 4区生物学

Brazilian Journal of Microbiology Pub Date : 2024-09-01 Epub Date: 2024-06-14 DOI: 10.1007/s42770-024-01411-0

Allysson Kayron de Carvalho Silva, Fernanda Jeniffer Lindoso Lima, Katia Regina Assunção Borges, Laís Araújo Souza Wolff, Marcelo Souza de Andrade, Rita de Nazaré Silva Alves, Carolina Borges Cordeiro, Marcos Antônio Custódio Neto da Silva, Maria do Desterro Soares Brandão Nascimento, Talita da Silva Espósito, Geusa Felipa de Barros Bezerra

{"title":"Utilization of Fusarium Solani lipase for enrichment of polyunsaturated Omega-3 fatty acids.","authors":"Allysson Kayron de Carvalho Silva, Fernanda Jeniffer Lindoso Lima, Katia Regina Assunção Borges, Laís Araújo Souza Wolff, Marcelo Souza de Andrade, Rita de Nazaré Silva Alves, Carolina Borges Cordeiro, Marcos Antônio Custódio Neto da Silva, Maria do Desterro Soares Brandão Nascimento, Talita da Silva Espósito, Geusa Felipa de Barros Bezerra","doi":"10.1007/s42770-024-01411-0","DOIUrl":"10.1007/s42770-024-01411-0","url":null,"abstract":"Omega-3 fatty acids, such as eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), offer numerous health benefits. Enriching these fatty acids in fish oil using cost-effective methods, like lipase application, has been studied extensively. This research aimed to investigate F. solani as a potential lipase producer and compare its efficacy in enhancing polyunsaturated omega-3 fatty acids with commercial lipases. Submerged fermentation with coconut oil yielded Lipase F2, showing remarkable activity (215.68 U/mL). Lipase F2 remained stable at pH 8.0 (activity: 93.84 U/mL) and active between 35 and 70 °C, with optimal stability at 35 °C. It exhibited resistance to various surfactants and ions, showing no cytotoxic activity in vitro, crucial for its application in the food and pharmaceutical industries. Lipase F2 efficiently enriched EPA and DHA in fish oil, reaching 22.1 mol% DHA and 23.8 mol% EPA. These results underscore the economic viability and efficacy of Lipase F2, a partially purified enzyme obtained using low-cost techniques, demonstrating remarkable stability and resistance to diverse conditions. Its performance was comparable to highly pure commercially available enzymes in omega-3 production. These findings highlight the potential of F. solani as a promising lipase source, offering opportunities for economically producing omega-3 and advancing biotechnological applications in the food and supplements industry.","PeriodicalId":9090,"journal":{"name":"Brazilian Journal of Microbiology","volume":" ","pages":"2211-2226"},"PeriodicalIF":2.1,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11405586/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141315956","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Equidae pythiosis in Brazil and the world: a systematic review of the last 63 years (1960-2023). 巴西和全世界的马科焦虫病：对过去 63 年（1960-2023 年）的系统回顾。

IF 2.1 4区生物学

Brazilian Journal of Microbiology Pub Date : 2024-09-01 Epub Date: 2024-07-05 DOI: 10.1007/s42770-024-01435-6

Daniela Isabel Brayer Pereira, Sônia Avila Botton, Lara Baccarin Ianiski, Caroline Quintana Braga, Aline Fontanella Maciel, Luíze Garcia Melo, Cristina Gomes Zambrano, Fábio Raphael Pascoti Bruhn, Janio Morais Santurio

{"title":"Equidae pythiosis in Brazil and the world: a systematic review of the last 63 years (1960-2023).","authors":"Daniela Isabel Brayer Pereira, Sônia Avila Botton, Lara Baccarin Ianiski, Caroline Quintana Braga, Aline Fontanella Maciel, Luíze Garcia Melo, Cristina Gomes Zambrano, Fábio Raphael Pascoti Bruhn, Janio Morais Santurio","doi":"10.1007/s42770-024-01435-6","DOIUrl":"10.1007/s42770-024-01435-6","url":null,"abstract":"This systematic review compiles reports of clinical pythiosis in horses, mules and donkeys from 1960 to 2023 worldwide, focusing on Brazil. We searched databases and included 71 articles detailing clinical characteristics, geographic distribution, epidemiology, diagnostic methods, therapies, and outcomes. The results showed that publications on equine pythiosis have significantly increased since 2010. Brazil reported the highest incidence, comprising 55% of cases, predominantly in the southern, northeastern, and central-western regions during summer and autumn. Cutaneous pythiosis was the most prevalent form, generally presenting as single lesions in the appendicular region, and affected females more than males. Diagnosis typically involved histopathology, used alone or with other methods. Various treatments have been employed, with surgery, often combined with chemotherapy and immunotherapy, being the most common. Notably, 80.84% of treated animals recovered, highlighting the effectiveness of these therapies in enhancing survival rates. The limitations of the study included the lack of data in published case reports, which made it difficult to collect and calculate epidemiological data. Additionally, we recognize that pythiosis in Brazil is underreported, since this disease does not have mandatory notification and several cases are not registered and/or reported in the literature. Lastly, it is hypothesized that equid pythiosis may be more widespread than currently known, and its real occurrence in Brazil remains uncertain.","PeriodicalId":9090,"journal":{"name":"Brazilian Journal of Microbiology","volume":" ","pages":"2969-2981"},"PeriodicalIF":2.1,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11405592/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141533606","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Long-term occurrence of multiple antimicrobial drug resistant Klebsiella pneumoniae isolates harboring virulent potential in a tertiary hospital from Brazil. 巴西一家三甲医院长期出现多种耐抗菌药物的肺炎克雷伯菌分离株，并具有致病潜能。

IF 2.1 4区生物学

Brazilian Journal of Microbiology Pub Date : 2024-09-01 Epub Date: 2024-05-14 DOI: 10.1007/s42770-024-01358-2

Ana Paula Cardoso Almeida, Miguel Augusto de Moraes, Amanda Kamyla Ferreira da Silva, Mariana Oliveira-Silva, Rafael Nakamura-Silva, Felipe Marques de Almeida, Georgios Joannis Pappas Junior, André Pitondo-Silva, Tatiana Amabile de Campos

{"title":"Long-term occurrence of multiple antimicrobial drug resistant Klebsiella pneumoniae isolates harboring virulent potential in a tertiary hospital from Brazil.","authors":"Ana Paula Cardoso Almeida, Miguel Augusto de Moraes, Amanda Kamyla Ferreira da Silva, Mariana Oliveira-Silva, Rafael Nakamura-Silva, Felipe Marques de Almeida, Georgios Joannis Pappas Junior, André Pitondo-Silva, Tatiana Amabile de Campos","doi":"10.1007/s42770-024-01358-2","DOIUrl":"10.1007/s42770-024-01358-2","url":null,"abstract":"Klebsiella pneumoniae strains are globally associated with a plethora of opportunistic and severe human infections and are known to spread genes conferring antimicrobial resistance. Some strains harbor virulence determinants that enable them to cause serious disease in any patient, both in the hospital and in the community. The aim of this study was to determine the frequency of antimicrobial resistance and virulence traits (by gene detection and string test) among 83 K. pneumoniae isolates obtained from patient cultures of a scholar tertiary hospital in the Midwestern Brazil (Brasília, DF). Antimicrobial susceptibility analysis showed that 94% (78/83) of the isolates presented one of the following resistance profiles: resistant (R, 39), multidrug-resistant (MDR, 29), or extensively drug-resistant (XDR, 10). Several MDR and XDR strains harbored multiple virulence genes and displayed hypermucoviscous phenotype. These characteristics were observed among isolates obtained throughout all the sample collection period (2013 - 2017). The K2 serotype gene, a molecular marker of hypervirulence, was detected in three isolates, one of which classified as XDR. Sequence typing revealed the occurrence of isolates belonged to high-risk (ST13) and multiple resistance-spreading clones (ST105). Thus, our findings showed the occurrence of virulent potential isolates that also presented MDR/XDR phenotypes from 2013 to 2015. This study also indicates the probable convergence of virulence and resistance since at least 2013 in Brazil.","PeriodicalId":9090,"journal":{"name":"Brazilian Journal of Microbiology","volume":" ","pages":"2313-2320"},"PeriodicalIF":2.1,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11405615/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140920216","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Unveiling the impact of selected essential oils on MRSA strain ATCC 33591: antibacterial efficiency, biofilm disruption, and staphyloxanthin inhibition. 揭示精选精油对 MRSA 菌株 ATCC 33591 的影响：抗菌效率、生物膜破坏和抑制钉螺黄素。

IF 2.1 4区生物学

Brazilian Journal of Microbiology Pub Date : 2024-09-01 Epub Date: 2024-05-22 DOI: 10.1007/s42770-024-01374-2

Fares Elghali, Ibtissem Ibrahim, Maha Guesmi, Fakher Frikha, Sami Mnif

{"title":"Unveiling the impact of selected essential oils on MRSA strain ATCC 33591: antibacterial efficiency, biofilm disruption, and staphyloxanthin inhibition.","authors":"Fares Elghali, Ibtissem Ibrahim, Maha Guesmi, Fakher Frikha, Sami Mnif","doi":"10.1007/s42770-024-01374-2","DOIUrl":"10.1007/s42770-024-01374-2","url":null,"abstract":"This work aimed to evaluate the effects of 4 selected essential oils on planktonic cells and microbial biofilms of the Staphylococcus aureus strain (MRSA ATCC 33591). The antibacterial activities of the four essential oils Geranium (Pelargonium graveolens), PgEO, Tea Tree (Melaleuca alternifolia) MaEO, Lemon peel (Citrus limon) ClEO and Peppermint (Mentha piperita) MpEO had MICs ranging from 1.56 to 12.5 µl/ml. The evaluation of the antibiofilm activities of the 4 EOs revealed that they had antiadhesive activities against S. aureus MRSA biofilms; the activity reached 60% (the EO of MpEO peppermint at a concentration of 3.12 µl/ml), and the eradication activity was 80% (the EO of PgEO and MpEO at 3.12 µl/ml). The antibiofilm activity of S. aureus has been explained by the binding of several essential oil bioactive molecules to the SarA protein, the main target protein involved in biofilm formation. The synthesis of the virulence factor staphyloxanthin by S. aureus MRSA ATCC 33591 was significantly inhibited in the presence of PgEO at a concentration of MIC/2. This inhibition was explained by the binding of the main PgEO molecules (β-citronellol and geraniol) to the CrTM protein involved in the staphyloxanthin synthesis pathway. There is evidence that these essential oils could be used as potential anti-virulents to control Staphylococcus biofilm formation.","PeriodicalId":9090,"journal":{"name":"Brazilian Journal of Microbiology","volume":" ","pages":"2057-2069"},"PeriodicalIF":2.1,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11405564/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141075276","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Anti-obesity effects of potential probiotic Lactobacillus strains isolated from Mongolian fermented dairy products in high-fat diet-induced obese rodent model. 从蒙古发酵乳制品中分离的潜在益生菌乳酸杆菌对高脂饮食诱导的肥胖啮齿动物模型的抗肥胖作用

IF 2.1 4区生物学

Brazilian Journal of Microbiology Pub Date : 2024-09-01 Epub Date: 2024-05-24 DOI: 10.1007/s42770-024-01372-4

Unurjargal Galindev, Uugantsetseg Erdenebold, Galindev Batnasan, Oyundelger Ganzorig, Batjargal Batdorj

{"title":"Anti-obesity effects of potential probiotic Lactobacillus strains isolated from Mongolian fermented dairy products in high-fat diet-induced obese rodent model.","authors":"Unurjargal Galindev, Uugantsetseg Erdenebold, Galindev Batnasan, Oyundelger Ganzorig, Batjargal Batdorj","doi":"10.1007/s42770-024-01372-4","DOIUrl":"10.1007/s42770-024-01372-4","url":null,"abstract":"This study aims to investigate the anti-obesity properties of lactic acid bacteria (LAB) isolated from fermented dairy products such as \"Airag\" and \"Khoormog\" in Mongolia. These traditional dairy products are widely used in Mongolia and believe in having potential probiotic, anti-diabetes, anti-cancer, and anti-tuberculosis properties and are made from unheated two-humped camel milk and mare milk, respectively. We chose three LAB strains based on their probiotic characteristics, including tolerance of gastric and bile acids. Then we checked the anti-obesity activity of probiotic strains in vivo. An animal model was evaluated in twenty male C57BL/6J mice by inducing obesity with a high-fat diet (HFD), which was divided into five groups: regular diet group (Negative control), HFD group (Positive control), HFD with Lacticaseibacillus paracasei X-1 (X-1), Lacticaseibacillus paracasei X-17 (X-17), and Limosilactobacillus fermentum BM-325 (BM-325). For six weeks, 5 × 109 colony-forming units (CFU) of bacteria were given orally to the LAB-fed groups. Fasting blood glucose (FBG), lipid profiles, organ index, and organ morphology were all measured. The probiotic strains suppressed growth in adipose cell volume, stabilized FBG, reduced liver cell degeneration, and slowed HFD-induced body weight gain. The results suggest that some strains increase general metabolism while lowering body weight.","PeriodicalId":9090,"journal":{"name":"Brazilian Journal of Microbiology","volume":" ","pages":"2501-2509"},"PeriodicalIF":2.1,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11405555/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141092897","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Influence of different culture media on the antimicrobial activity of Pediococcus pentosaceus ST65ACC against Listeria monocytogenes. 不同培养基对五味 Pediococcus ST65ACC 对李斯特菌抗菌活性的影响。

IF 2.1 4区生物学

Brazilian Journal of Microbiology Pub Date : 2024-09-01 Epub Date: 2024-05-24 DOI: 10.1007/s42770-024-01391-1

Francielly Soares Oliveira, Rafaela da Silva Rodrigues, Valéria Quintana Cavicchioli, Antônio Fernandes de Carvalho, Luís Augusto Nero

{"title":"Influence of different culture media on the antimicrobial activity of Pediococcus pentosaceus ST65ACC against Listeria monocytogenes.","authors":"Francielly Soares Oliveira, Rafaela da Silva Rodrigues, Valéria Quintana Cavicchioli, Antônio Fernandes de Carvalho, Luís Augusto Nero","doi":"10.1007/s42770-024-01391-1","DOIUrl":"10.1007/s42770-024-01391-1","url":null,"abstract":"Pediococcus pentosaceus ST65ACC is a bacteriocinogenic lactic acid bacteria (LAB) isolated from Brazilian artisanal cheese that is capable of inhibiting different food pathogens, mainly Listeria monocytogenes. The production of bacteriocins can be influenced by several growth conditions, such as temperature, pH, and medium composition. This study aimed to evaluate the effect of different culture media on the production of bacteriocins and antimicrobial activity of P. pentosaceus ST65ACC on L. monocytogenes Scott A. The strains were inoculated alone and in coculture in four different media: BHI broth, MRS broth, meat broth, and reconstituted skim milk (RSM) 10% (w/v). The culture media were then incubated at 37 °C for 96 h, and count analysis, pH measurement, and bacteriocin production were performed at 0, 24, 48, 72 and 96 h. L. monocytogenes was inhibited to nondetectable levels in coculture with P. pentosaceus ST65ACC in MRS broth within 96 h, consistent with the high production of bacteriocin throughout the analysis period (3,200-12,800 AU/mL). However, lower inhibitory activities of P. pentosaceus ST65ACC on L. monocytogenes Scott A were recorded in BHI, RSM, and meat broth, with low or no production of bacteriocins at the analyzed times. The composition of these culture media may have repressed the production and activity of bacteriocins and, consequently, the antagonist activity of P. pentosaceus ST65ACC on L. monocytogenes Scott A. The results showed that the antimicrobial activity was more effective in MRS broth, presenting greater production of bacteriocins and less variability when compared to the other media analyzed.","PeriodicalId":9090,"journal":{"name":"Brazilian Journal of Microbiology","volume":" ","pages":"2539-2545"},"PeriodicalIF":2.1,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11405628/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141092970","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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