Brazilian Journal of Microbiology最新文献

Evaluation of the potential virulence of Mycobacterium avium subspecies paratuberculosis isolates from cattle in Uganda. 乌干达牛禽分枝杆菌亚种副结核分离株潜在毒力的评估。

IF 1.9 4区生物学

Brazilian Journal of Microbiology Pub Date : 2026-05-06 DOI: 10.1007/s42770-026-01951-7

Judah Ssekitoleko, Franziska Tanneberger, Kamal H Eltom, ElSagad Eltayeb, Uwe Truyen, Lonzy Ojok, Ahmad Amanzada, Edward Wampande, Moses Luutu Nsubuga, Peregrine Sebulime, Ahmed Abd El Wahed, Julius Boniface Okuni

{"title":"Evaluation of the potential virulence of Mycobacterium avium subspecies paratuberculosis isolates from cattle in Uganda.","authors":"Judah Ssekitoleko, Franziska Tanneberger, Kamal H Eltom, ElSagad Eltayeb, Uwe Truyen, Lonzy Ojok, Ahmad Amanzada, Edward Wampande, Moses Luutu Nsubuga, Peregrine Sebulime, Ahmed Abd El Wahed, Julius Boniface Okuni","doi":"10.1007/s42770-026-01951-7","DOIUrl":"10.1007/s42770-026-01951-7","url":null,"abstract":"Understanding the pathogenesis of paratuberculosis (PTB), caused by Mycobacterium avium subspecies paratuberculosis (MAP), is essential in the management of this economically important disease. MAP strains are known to differ genotypically, which could contribute to differences in strain virulence and disease outcomes. The behaviour of MAP inside the host and the mechanisms utilized by MAP to overcome the host defence system are not entirely understood. This study aimed at evaluating the potential virulence of different MAP isolates from cattle in Uganda. This was achieved by analysing the cytokine expression profiles of RAW 264.7 macrophages when infected with four different MAP isolates: MAP1, MAP2, MAP3 and MAP4. Specific real-time PCR was used to determine the expression of different cytokines, probable virulence genes of MAP as well as MAP quantities inside the macrophages at different time points (3 h, 24 h, 48 h) post-infection (pi). There were considerable variations in the cytokine expressions among macrophages infected with the different MAP isolates. The expression of IL-6 in macrophages infected with MAP1 at 48 h pi was noticeably high compared to the other isolates; and this is a known strategy for MAP survival inside macrophages. The regulation of the probable virulence genes: kdpC, katG, papA2, impA, umaA1, and the anti-apoptotic (BCL-2) gene also varied considerably among the isolates. Isolates MAP1, MAP2 and MAP3 demonstrated higher virulent behaviour regarding intracellular replication and survival compared to MAP4 isolate. The behaviour of these MAP isolates clearly indicates variations in virulence and further research needs to focus on how this can be exploited in the design of PTB diagnostic and control strategies.","PeriodicalId":9090,"journal":{"name":"Brazilian Journal of Microbiology","volume":"57 1","pages":""},"PeriodicalIF":1.9,"publicationDate":"2026-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13149813/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147833412","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Frequency and characterization of β-lactam-resistant Escherichia coli from wild birds from Marmara Region in Turkey. 土耳其马尔马拉地区野生鸟类β-内酰胺耐药大肠埃希菌的频率和鉴定

IF 1.9 4区生物学

Brazilian Journal of Microbiology Pub Date : 2026-05-06 DOI: 10.1007/s42770-026-01941-9

Barış Halaç, Ayşe Ilgın Kekeç, Baran Çelik, Deniz Kesen, Beren Başaran Kahraman, Belgi Diren Sığırcı, Arzu Funda Bağcıgil, Kemal Metiner, Yavuz Çokal, Seyyal Ak

{"title":"Frequency and characterization of β-lactam-resistant Escherichia coli from wild birds from Marmara Region in Turkey.","authors":"Barış Halaç, Ayşe Ilgın Kekeç, Baran Çelik, Deniz Kesen, Beren Başaran Kahraman, Belgi Diren Sığırcı, Arzu Funda Bağcıgil, Kemal Metiner, Yavuz Çokal, Seyyal Ak","doi":"10.1007/s42770-026-01941-9","DOIUrl":"10.1007/s42770-026-01941-9","url":null,"abstract":"This study aimed to phenotypically and genotypically detect beta-lactam-resistant Escherichia coli in the feces of wild and migratory birds residing in or passing through the Marmara Region during seasonal migration. A total of 272 fresh fecal samples were non-invasively collected and grouped as follows: (1) resident wild waterfowl, (2) resident urban birds (e.g., pigeons, crows, sparrows), (3) migratory birds overwintering in Turkey, and (4) migratory birds staying during spring and summer. Samples were cultured on MacConkey agar with and without cefotaxime (1 mg/L). Carbapenem resistance was assessed using the Modified Hodge Test; none of the 212 isolates tested positive. Among the 272 birds sampled, 62 (22.8%) carried ESBL and/or AmpC producing isolates. Antimicrobial-resistant E. coli were identified in 59 of 272 birds (21.7%), including 47 ESBL-positive, 6 AmpC-positive, and 5 harboring both resistance types. Group 1 (mainly gulls) showed the highest resistance rates. PCR analysis revealed that among 84 isolates, 48 carried only beta-lactamase genes, 3 only AmpC genes, and 5 both. Detected genes included: blaCTX-M (n = 50), blaSHV (n = 2), and blaOXA10 (n = 10); for AmpC: blaMOX (n = 6) and blaCIT (n = 2). Species-specific results confirmed the highest frequency of resistance genes in gulls. The Frequency of resistant isolates was significantly higher in Groups 1 and 2 (P < 0.001). The resistance profiles identified in gulls especially those feeding at landfills or in direct contact with human, animal, and agricultural waste suggest a likely anthropogenic and/or zoonotic origin of the beta lactam resistance observed in this study conducted in Marmara Region.","PeriodicalId":9090,"journal":{"name":"Brazilian Journal of Microbiology","volume":"57 1","pages":""},"PeriodicalIF":1.9,"publicationDate":"2026-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13149730/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147833397","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Integrated Morphological, Functional, and Genomic Analysis of a Novel Lytic Phage (VIT_AJPSC) Infecting Multidrug-Resistant Shigella dysenteriae. 一种感染多重耐药痢疾志贺氏菌的新型裂解噬菌体（VIT_AJPSC）的形态、功能和基因组分析。

IF 1.9 4区生物学

Brazilian Journal of Microbiology Pub Date : 2026-05-06 DOI: 10.1007/s42770-026-01947-3

Aishwarya Srivastava, Jayaprakash N S

{"title":"Integrated Morphological, Functional, and Genomic Analysis of a Novel Lytic Phage (VIT_AJPSC) Infecting Multidrug-Resistant Shigella dysenteriae.","authors":"Aishwarya Srivastava, Jayaprakash N S","doi":"10.1007/s42770-026-01947-3","DOIUrl":"10.1007/s42770-026-01947-3","url":null,"abstract":"Bacteria are historically known to cause a disproportionate number of deaths because of infectious diseases worldwide. Annually, 55 million deaths are accounted for because of bacterial infections, out of which approximately 3 million deaths are because of diarrhoea and dysentery. Shigellosis is an infectious disease caused by Shigella species, and it targets the intestinal tract. It is characterised by acute diarrhoea mixed with blood. The oral antibiotics recommended for shigellosis are ciprofloxacin and azithromycin, with ciprofloxacin as the first-line treatment. Recently, Shigella species have shown a rapid increase in resistance to these antibiotics, which is leading to overdose and prolonged treatment for the same. There is a need of the hour to find a potential substitute for antibiotics. Many alternatives, such as antimicrobial peptides, plant-based substances, bacteriophages, and probiotics, have shown promise compared to antibiotics. This paper focuses on the isolation and characterization of bacteriophages against Shigella dysenteriae. Bacteriophages are viruses that kill bacteria and are very specific to their host. They require host machinery to replicate; therefore, there is very little chance of developing resistance in bacteria against phage. The paper describes screening and isolation of phages from environmental samples against Shigella dysenteriae, followed by optimization studies and their characterization, such as host range, burst size and one-step growth curve. Morphological characterization using transmission electron microscopy (TEM) and whole genome sequencing has been performed, indicating that the phage belongs to the Myoviridae family and has a genome size of 38kbp.","PeriodicalId":9090,"journal":{"name":"Brazilian Journal of Microbiology","volume":"57 1","pages":""},"PeriodicalIF":1.9,"publicationDate":"2026-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13149728/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147833383","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Optimization of cellulase production by Albifimbria verrucaria TRQ88 and its application in saccharification of cellulose pulp from wheat straw. 疣状白弧菌TRQ88产纤维素酶的优化及其在麦草纤维素浆糖化中的应用。

IF 1.9 4区生物学

Brazilian Journal of Microbiology Pub Date : 2026-05-03 DOI: 10.1007/s42770-026-01953-5

Alma Marisol Badilla-Ruíz, Sara Roxana Maldonado-Bustamante, Iram Mondaca-Fernández, José de Jesús Balderas-Cortés, María Mercedes Meza-Montenegro, Héctor Arvayo-Enríquez

引用次数: 0

Effects of light colors on the biochemical composition of Scenedesmus obliquus. 不同光色对斜花菜生化组成的影响。

IF 1.9 4区生物学

Brazilian Journal of Microbiology Pub Date : 2026-05-03 DOI: 10.1007/s42770-026-01957-1

Vanessa Campos Guedes, Jaqueline Carmo da Silva, Antonio Carlos Luperni Horta, Ana Teresa Lombardi

{"title":"Effects of light colors on the biochemical composition of Scenedesmus obliquus.","authors":"Vanessa Campos Guedes, Jaqueline Carmo da Silva, Antonio Carlos Luperni Horta, Ana Teresa Lombardi","doi":"10.1007/s42770-026-01957-1","DOIUrl":"10.1007/s42770-026-01957-1","url":null,"abstract":"This study explores the impact of different light colors on the population growth, photosynthesis, and biochemical composition of Scenedesmus obliquus. Literature shows various biotechnological solutions to mitigate the environmental problems caused by greenhouse gas emissions from environmentally unsustainable processes, and microalgae cultures have emerged as a promising technology with the potential to support diverse production chains. In this context, Scenedesmus has demonstrated considerable potential. The present study presents a straightforward approach to enhance protein production in S. obliquus through the strategic manipulation of light wavelengths, with the potential for practical implementation. The paper goes on to outline future research directions for integrating light control technologies, emphasizing the potential for maximizing biomolecules accumulation. Cell density and viability, growth rates, carbohydrates and proteins, total carotenoids and photosynthesis inferred by pulse amplitude modulated fluorescence (PhytoPAM) were evaluated. Blue light was identified as optimal for total proteins production (5.5 µg/mL vs. 2.5-3.8 µg/mL in other light colors), while white light showed promise for carotenoid accumulation (0.160 µg/mL vs. 0.10-0.13 in other light colors). Notably, the observed variations in the light spectrum did not result in any significant changes in the maximum growth rates (1.08-1.13), underscoring the robustness of the organism under study. Photosynthetic parameters remained unaffected by light quality, indicating a robust photosynthetic machinery (qP 1.0, NPQ 0.2, and effective quantum yield 0.58). These findings contribute to our understanding of algal biology and provide practical insights for biotechnological applications, paving the way for sustainable solutions.","PeriodicalId":9090,"journal":{"name":"Brazilian Journal of Microbiology","volume":"57 1","pages":""},"PeriodicalIF":1.9,"publicationDate":"2026-05-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13136458/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147810720","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Optimization, partial characterization, and application of a biosurfactant produced by the wheat endophytic Bacillus sp. WL2-23. 小麦内生芽孢杆菌WL2-23生物表面活性剂的优化、部分表征及应用

IF 1.9 4区生物学

Brazilian Journal of Microbiology Pub Date : 2026-04-30 DOI: 10.1007/s42770-026-01952-6

Anamika Jha, Srushti Kachhiya, Shivangikumari Chaudhari, Nishita Master, Janki K Patel

引用次数: 0

Chemical profiling and cytotoxic activity of the endophytic fungus Epicoccum sorghinum isolated from Disynaphia filifolia. 荆芥内生真菌高粱表occum的化学特性及细胞毒活性。

IF 1.9 4区生物学

Brazilian Journal of Microbiology Pub Date : 2026-04-29 DOI: 10.1007/s42770-026-01927-7

Anderson Valdiney Gomes Ramos, Nathalia da Silva Malaco, Rodolfo Bento Balbinot, Drielli Rhiane Peres Colhado Areas, Francielli Alana Pereira Valeze, Camila Botin Francisco, Jesieli Beraldo Borrazzo, Andressa Domingos Polli, Julio Cesar Polonio, Celso Vataru Nakamura, João Alencar Pamphile, Ernani Abicht Basso, Maria Helena Sarragiotto, Debora Cristina Baldoqui

引用次数: 0

Whole genome-sequence-based molecular characterisation of Salmonella Kentucky co-resistant to fluoroquinolones and cephalosporins isolated from children during 2017 to 2024: emergence of bla_CTX-M-55 harbouring Salmonella Kentucky in India. 2017年至2024年从儿童中分离的肯塔基沙门氏菌对氟喹诺酮类药物和头孢菌素共耐药的全基因组序列分子特征：印度出现携带肯塔基沙门氏菌的blaCTX-M-55

IF 1.9 4区生物学

Brazilian Journal of Microbiology Pub Date : 2026-04-29 DOI: 10.1007/s42770-026-01950-8

Paulami Dutta, Gourab Halder, Priyanka Denny, Arindam Ganai, Shanta Dutta

引用次数: 0

Evaluating the presence of bovine coronavirus in diarrheic calves in Lahore, Punjab, Pakistan: a food security risk. 评估巴基斯坦旁遮普省拉合尔腹泻小牛中牛冠状病毒的存在：粮食安全风险

IF 1.9 4区生物学

Brazilian Journal of Microbiology Pub Date : 2026-04-27 DOI: 10.1007/s42770-026-01931-x

Saima Abbas, Shahan Azeem, Sohail Raza, Sehrish Firyal

引用次数: 0

Ups and downs in the experimental treatment of murine typhoid fever using gentamicin combined with caffeine. 庆大霉素联合咖啡因治疗小鼠伤寒的实验研究。

IF 1.9 4区生物学

Brazilian Journal of Microbiology Pub Date : 2026-04-27 DOI: 10.1007/s42770-026-01948-2

Esther de Souza Silva, Roseane Thays Dos Santos Rocha, Joyce Nayara Gomes da Silva, Victor Vinicius Pereira Ribeiro, Islana Silva Ponte, Isabela Cristina Bandeira Fraga, Camila Sant Ana de Oliveira, Viviane Moreira Lima Galvão, Alison Hideo Jolo Silva, Valdemiro Amaro da Silva Junior, José Vitor Lima-Filho

引用次数: 0