Brazilian Journal of Microbiology最新文献

{"title":"Comparative analysis of two immunosuppression protocols for vulvovaginal candidiasis induction in Wistar rats.","authors":"Félix Roman Munieweg, Thais Pinheiro Ribeiro, Jean Ramos Boldori, Nathane Rosa Rodrigues, Nicolle Lauay Carvalho Sanches Hoesel, Ana Luiza Reetz Polleto, Cheila Denise Ottonelli Stopiglia, Sandra Elisa Haas, Cristiane Casagrande Denardin","doi":"10.1007/s42770-025-01661-6","DOIUrl":"https://doi.org/10.1007/s42770-025-01661-6","url":null,"abstract":"<p><p>Vulvovaginal candidiasis (VVC) is a prevalent public health problem worldwide. The frequent recurrences and treatment failures associated with existing therapies highlight the urgent need to explore new potential treatments. However, protocols for inducing vulvovaginal candidiasis in rodents are limited and lack standardization. Most approaches rely on immunosuppression to mimic human conditions. This study aimed to compare two immunosuppression protocols in a vulvovaginal candidiasis infection model using female Wistar rats. The animals were subjected to two immunosuppression: Chemical immunosuppression using cyclophosphamide (IMS) and a chronic unpredictable mild stress model (CUMS). Following the immunosuppression period, the rats were inoculated with C. albicans ATCC10231. After confirming the infection, the animals were treated with a vaginal cream containing clotrimazole (10 mg/g) or a vehicle for 7 days. During this period, behavioral parameters, food consumption, body weight, and vaginal microbial load were evaluated. At the end of treatment, the animals were euthanized, and blood, histological tissue, and microbiological parameters were analyzed. Animals subjected to the CUMS protocol exhibited significant behavioral changes, reduced food consumption, and impaired weight gain. They also displayed hematological and histological alterations, indicating severe immunosuppression and infection. Clotrimazole treatment failed to cure all animals in this group. In contrast, animals in the IMS protocol experienced milder immunosuppression, and clotrimazole treatment successfully cured all treated animals. The IMS method is more suitable for investigating primary vulvovaginal candidiasis infection compared to the CUMS method. The CUMS protocol induced more pronounced changes, such as weight loss and behavioral alterations, which may not accurately reflect primary VVC in humans. The IMS method, however, resulted in a vaginal infection with minimal systemic changes, more closely resembling the condition observed in women with primary VVC.</p>","PeriodicalId":9090,"journal":{"name":"Brazilian Journal of Microbiology","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143779109","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Utilizing actinobacteria for glyphosate biodegradation: innovative solutions for sustainable agricultural soil remediation.","authors":"Hadjer Rebai, Cherifa Lefaida, Essam Nageh Sholkamy, Parkasam Thanka Pratheesh, Ashraf Aly Hassan, Djaber Tazdait, Thavasimuthu Citarasu, Allaoueddine Boudemagh","doi":"10.1007/s42770-025-01655-4","DOIUrl":"https://doi.org/10.1007/s42770-025-01655-4","url":null,"abstract":"<p><p>Glyphosate is one of the most widely used herbicides globally, yet its extensive application has raised significant ecological concerns. The objective of this study is to evaluate the ability of actinobacteria to degrade glyphosate under various environmental conditions. Four strains of actinobacteria were selected for their ability to thrive in a minimal medium containing 50 mg/L of glyphosate. The optimization of glyphosate biodegradation was assessed through a colorimetric method, which showed that the highest biodegradation rate occurred at a pH of 7.2, a temperature of 30 °C and an inoculum volume of 4%. The isolates were identified as follows: Streptomyces sp. strain SPA2 (accession number pp413753), Streptomyces rochei. strain IT (accession number pp413751), Streptomyces variabilis. strain Herb (accession number pp413750), and Streptomyces griseoincarnatus. strain SC (accession number PP413754). Analysis of total organic carbon reduction demonstrated that the strains SPA2, IT, Herb, and SC achieved reductions of 56.11%, 47.96%, 82.06%, and 67.12%, respectively. Furthermore, ATR-FTIR spectroscopy indicated alterations in the chemical structure of glyphosate post-biodegradation. These findings underscore the significant potential of the identified actinobacterial strains as viable agents for the bioremediation of glyphosate-contaminated agricultural soils.</p>","PeriodicalId":9090,"journal":{"name":"Brazilian Journal of Microbiology","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143779146","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bartonella spp., Coxiella burnetii and Rickettsia rickettsii in urban bats: molecular investigation of neglected zoonoses in Brazil.","authors":"Danilo Alves de França, Alana Vitor Barbosa da Costa, Leandro Meneguelli Biondo, Ana Íris de Lima Duré, Benedito Donizete Menozzi, Felipe Fornazari, Helio Langoni","doi":"10.1007/s42770-025-01660-7","DOIUrl":"https://doi.org/10.1007/s42770-025-01660-7","url":null,"abstract":"<p><p>In the last decade, cases of bartonellosis, Q fever and Brazilian spotted fever have been recorded in Brazil. Despite this, their occurrence is still underestimated. This study aimed to investigate Bartonella spp., Coxiella burnetii and Rickettsia rickettsii DNA in organs of urban bats collected from the São Paulo state, southeastern Brazil. Spleen and liver samples were collected from 102 bats from urban areas and evaluated by real-time PCR. Positive samples were submitted to conventional PCR and subsequent sequencing for species identification. In total, 3.9% of the bats were positive for Bartonella spp. in the real-time PCR and 2.9% were sequenced. Of the positive bats, two were Artibeus lituratus and two were Myotis nigricans. The sequences obtained were analyzed using the BLASTn, identified as B. koehlerae (n = 2) and B. clarridgeiae (n = 1) and deposited in Genbank. A close phylogenetic relationship was observed between the bat isolates in the study and global and Brazilian isolates from cats and humans. No sample in the study was positive for C. burnetii or R. rickettsii. The presence of Bartonella associated with infection of cats and humans in Brazilian bats reflects the neglected state of the disease in the country and the need for epidemiological surveillance actions. Cat scratch disease is currently a public health problem in Brazil and needs to be tracked.</p>","PeriodicalId":9090,"journal":{"name":"Brazilian Journal of Microbiology","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143708459","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cyphellophora denticulata sp. nov. a new dark septate endophyte from banana roots in Brazil.","authors":"Jaciara Dos Santos Santana, Fábio Alex Custódio, Jaqueline Aparecida de Oliveira, Olinto Liparini Pereira","doi":"10.1007/s42770-025-01654-5","DOIUrl":"https://doi.org/10.1007/s42770-025-01654-5","url":null,"abstract":"<p><p>The genus Cyphellophora includes fungal species with dark mycelia and diverse lifestyles. These morphological characteristics are similar to those of dark septate endophytes (DSEs), which are strict root fungi. However, DSEs are rarely reported in economically important crops such as bananas. The present study aimed to describe dark septate endophytes belonging to Cyphellophora isolated from healthy banana plants in Brazil. The isolates were obtained from healthy banana roots of the Prata Anã, Prata Catarina, and Nanica cultivars. Posteriorly, the fungal isolates were submitted for phylogenetic and morphological analyses. Phylogenetic analysis was performed based on the Internal transcribed spacer (ITS), Large subunit ribosomal ribonucleic acid (LSU rRNA) and Beta-tubulin gene (tub2). The morphological analysis was performed using four culture media: Malt Extract Agar, Oatmeal Agar, Potato Carrot Agar and Potato Dextrose Agar. The fungal isolates were cultivated for three weeks in the dark at 25 °C and 37 °C. Based on morphological and phylogenetic analyses, the isolates represents a new Cyphellophora species, namely C. denticulata. The new species has some morphological characteristics different from those shared by the most species of the genus Cyphellophora, such as the denticles presence in the conidiogenous cells. In this study, we report for the first time a Cyphellophora species isolated from banana plants as dark septate endophyte and provided a key for Cyphellophora species.</p>","PeriodicalId":9090,"journal":{"name":"Brazilian Journal of Microbiology","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143708460","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Anellovirus species in the serum of acute febrile patients from Mato Grosso, central Western Brazil, 2019.","authors":"Eduarda Pavan, Janeth Aracely Ramirez Pavón, Márcio Roberto Teixeira Nunes, Millena Moreira Carrasco, Marcelo Adriano Mendes Dos Santos, Renata Dezengrini Slhessarenko","doi":"10.1007/s42770-025-01658-1","DOIUrl":"https://doi.org/10.1007/s42770-025-01658-1","url":null,"abstract":"<p><p>The Anelloviridae family is one of the most diverse group of viruses within human virome, comprising 155 species distributed across 30 genera, four of which infect humans. Using a metagenomic approach to examine the frequency and diversity of viruses in the serum of patients with acute febrile illness in Mato Grosso during 2019, we identified and characterized two complete, three nearly complete and nine partial anellovirus genomes, including eleven putatively new species. Alphatorquevirus was the most prevalent genus (50%; n = 7), followed by Gammatorquevirus (35.7%; n = 5) and Betatorquevirus (14.3%; n = 2), consistent with global reports showing its dominance within Anelloviridae. Additionally, 65% of genomes were recovered from children, supporting higher anellovirus diversity and load in younger individuals.</p>","PeriodicalId":9090,"journal":{"name":"Brazilian Journal of Microbiology","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143708457","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The effect of different culture mediums on the morphological characters, growth parameters, chemical contents, and biological activities of Kamptonema formosum (Bory ex Gomont) Strunecký, Komárek & J. Smarda.","authors":"Alican Bahadir Semerci, Ayşe Gül Tekbaba, Tuğba Ongun Sevindik","doi":"10.1007/s42770-025-01657-2","DOIUrl":"https://doi.org/10.1007/s42770-025-01657-2","url":null,"abstract":"<p><p>In this study, the effects of different culture mediums (Spirulina, BG11 and F/2) on Kamptonema formosum strain were evaluated in terms of morphological characters and colonial structure, growth parameters (OD, chlorophyll-a), pH, dry biomass, biochemical content (total proteins, total carbohydrates, total lipids, and total phenolic contents) and biological activities (antioxidant and antibacterial activity). Based on the light and electron microscope observations, variations were detected in the morphological characters and colonial structures of the Cyanobacteria grown in the three culture mediums. K. formosum grown in Spirulina medium developed more than those grown in BG11 and F/2 mediums according to OD560, OD680, chlorophyll-a, and pH. The dry biomass, total protein, and total lipid content of the strain produced in the Spirulina medium were found to be significantly higher (p < 0.05) compared to other mediums. The methanolic extract obtained from the biomass grown in Spirulina medium showed strong antibacterial activity on Staphylococcus aureus (16.5 mm) and Enterecoccus faecalis (15 mm). In addition, the highest DPPH scavenging activity and total phenolic content were determined in the extract obtained from the Spirulina medium, while the lowest was detected in the extract obtained from the F/2 medium. As a result, K. formosum developed in Spirulina medium proved to be more effective in obtaining biomass, revealing biochemical contents and pharmacological activities.</p>","PeriodicalId":9090,"journal":{"name":"Brazilian Journal of Microbiology","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143708466","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genomic and phenotypic insights into Serratia interaction with plants from an ecological perspective.","authors":"Ubiana C Mourão Silva, Daliane R C da Silva, Sara Cuadros-Orellana, Leandro M Moreira, Laura R Leite, Julliane D Medeiros, Erica B Felestrino, Washington L Caneschi, Nalvo F Almeida, Robson S Silva, Christiane A Oliveira-Paiva, Vera Lúcia Dos Santos","doi":"10.1007/s42770-025-01652-7","DOIUrl":"https://doi.org/10.1007/s42770-025-01652-7","url":null,"abstract":"&lt;p&gt;&lt;p&gt;We investigated the plant growth-promoting potential of two endophytic strains of Serratia marcescens, namely SmCNPMS2112 and SmUFMG85, which were isolated from the roots of the same maize (Zea mays) plant. The strains were evaluated in vitro for their ability to produce siderophores and indoleacetic acid, form biofilm, solubilize iron phosphate (Fe-P) and Araxá rock phosphate (RP), mineralize phytate, and for their ability to adhere and colonize host roots. Additionally, their plant growth-promoting potential was tested in vivo under greenhouse conditions using millet grown in soil under two fertilization schemes (triple superphosphate, TSP, or commercial rock phosphate, cRP). Both strains improved at least five physiological traits of millet or P content in soil. In order to elucidate the genetic basis of the plant growth-promoting ability of these strains, their genomes were compared. While both genomes exhibited a similar overall functional profile, each strain had unique features. SmCNPMS2112 contained genes related to arsenic and aromatic hydrocarbons degradation, whereas SmUFMG85 harbored genes related to rhamnolipid biosynthesis and chromium bioremediation. Also, we observe a unique repertoire of genes related to plant growth-promotion (PGP) in the SmUFMG85 genome, including oxalate decarboxylase (OxdC), associated with the catabolism of oxalic acid, and aerobactin siderophore (lucD) in the genome of SmCNPMS2112. The alkaline phosphatase was observed on two strains, but acid phosphatase was exclusive to SmUFMG85. Eighteen secondary metabolic gene clusters, such as those involved in the biosynthesis of macrolides and bacillomycin, among others, occur in both strains. Moreover, both genomes contained prophages, suggesting that viral-mediated horizontal gene transfer may be a key mechanism driving genomic variability in the endophytic environment. Indeed, the most genes unique and accessory of SmUFMG85 and SmCNPMS2112 were localized in genomic islands, highlighting genome plasticity and its underlying drivers. To investigate the ecological distribution of plant-interaction traits in the genus Serratia, the genomes of SmUFMG85 and SmCNPMS2112 strains were compared with those of other 19 Serratia strains of different species, which were isolated from different environments. We observe that many features for PGP are present in all genomes, regardless of niche, for instance: formation of flagella, fimbriae and pili, chemotaxis, biosynthesis of siderophores, indole-3-acetic acid (IAA) and volatile organic (VOC) and inorganic (VIC) compounds, such as acetoin and HCN. Also, all the analyzed genomes show an antimicrobial resistance repertoire of genes that confer resistance to several antibiotics belonging to the groups of aminoglycosides and quinolones, for instance. Also, from a niche partitioning perspective, secretion system preference and the ability to produce exopolysaccharides involved in biofilm formation are among the features that v","PeriodicalId":9090,"journal":{"name":"Brazilian Journal of Microbiology","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143708462","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characteristics and virulence gene profiles of a pathogenic Lactococcus garvieae isolated from diseased Macrobrachium rosenbergii.","authors":"Anirban Paul, Samikshya Parida, Snatashree Mohanty, Subham Biswal, Bindu R Pillai, Debabrata Panda, Pramoda Kumar Sahoo","doi":"10.1007/s42770-025-01653-6","DOIUrl":"https://doi.org/10.1007/s42770-025-01653-6","url":null,"abstract":"<p><p>Macrobrachium rosenbergii is a commercially important freshwater prawn cultured on a large scale mostly in south and south east Asian countries. Diseases are one of the bottlenecks for the successful culture and production of this important species. Lactococcus garvieae is a Gram-positive coccus commonly found in aquatic environments causing fish and shellfish diseases. In the present investigation, we have isolated and characterized L. garvieae, as etiological agent of white muscle disease in freshwater prawn juveniles. The infected prawn samples showing clinical signs of opaque and whitish muscles, sluggishness and mortality were collected and processed. The isolated bacterium was identified using biochemical methods and 16s rDNA sequencing and species-specific PCR. The sequence obtained revealed > 99% identity with L. garvieae reported elsewhere. Koch's postulate was experimentally established through intramuscular challenge and the infected prawn muscle revealed massive coagulative necrosis with the presence of cocci. The isolate was found to be resistant to antibiotics namely clindamycin, cefoxitin, amoxicillin and co-trimoxazole. The heavy metal tolerance assay revealed the isolate to be tolerant to Cu²⁺ and Cr⁶⁺ and less tolerant to Hg²⁺. Class I integron was also identified in L. garvieae isolate. Further, the isolate was screened for several virulence genes and found to have hemolysins 1, 2, and 3, adhesin PsaA, adhesin Pav, enolase, LPxTG 1, 3, and 4, adhesin clusters 1 and 2, and adhesin in the PCR assay. It is the first report of L. garvieae infection in freshwater prawns in India and will pave the way for developing suitable preventive measures for future sustainable culture and production of this important aquaculture species.</p>","PeriodicalId":9090,"journal":{"name":"Brazilian Journal of Microbiology","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143656086","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Investigation of anti-microbial and cytotoxic potential of Streptomyces werraensis GRS9 derived from the sediments of river Ganga.","authors":"Deepa Malik, Himalaya Panwar, Swati Rani, Kartikey Kumar Gupta","doi":"10.1007/s42770-025-01642-9","DOIUrl":"https://doi.org/10.1007/s42770-025-01642-9","url":null,"abstract":"<p><p>The aim of the present work was to screen bacterial and actinomycetes strains from the sediments of river Ganga (India) as a promising source of anti-microbial and anti-cancer agents along with spectroscopic and chromatographic identification of bio-active compounds. The strain GRS9 exhibited broad-spectrum bio-activity against all the 15-test organisms incorporated in our study with minimum inhibitory concentrations (MICs) ranging from 16 µg/ml against Staphylococcus aureus MTCC3160 to 500 μg/ml for Escherichia coli (MTCC118). The cytotoxic profile of ethyl acetate extract was also evaluated against Human Colon Cancer Cell Line (HCT116) by Neutral Red Uptake (NRU) assay, followed by in silico study to determine its pre-qualification for drug suitability. The results indicated that Streptomyces werraensis GRS9 extract possessed anti-cancer properties (IC₅₀ = 22.95 µg/ml) and found suitable for further drug development as reflected in Absorption, Distribution, Metabolism and Excretion (ADME) prediction having no violation of Lipinski's rule of five. Bioactive compounds associated with GRS9 were identified by Gas Chromatography Mass Spectroscopy (GC-MS) and Fourier Transform Infrared Spectroscopy (FTIR), revealing 29 compounds along with 10 major compounds identified via National Institute of Standards and Technology (NIST) /Wiley library. These compounds include N-(4-methyl-1-Piperzinyl)-1-Napthamide (a compound of immense pharmacological potential especially in oncology) along with anti-microbials i.e. Dodecanamide and 1,2-Benzenedicarboxylic acid, Diethyl ester. The findings revealed our sediment isolate Streptomyces werraensis GRS9 to be a suitable candidate for the isolation and purification of bio-active compounds that may act as a source of anti-microbial and anti-cancer agents.</p>","PeriodicalId":9090,"journal":{"name":"Brazilian Journal of Microbiology","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143596225","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Insights into fungal diversity and dynamics of vaginal mycobiota.","authors":"Liza Ouarabi, Bernard Taminiau, Georges Daube, Nacim Barache, Farida Bendali, Djamel Drider, Anca Lucau-Danila","doi":"10.1007/s42770-025-01650-9","DOIUrl":"https://doi.org/10.1007/s42770-025-01650-9","url":null,"abstract":"<p><p>Although less studied than its bacterial counterpart, the fungal component of the vaginal microbiota plays a critical role in maintaining vaginal homeostasis. Most research on the composition of the vaginal mycobiota has focused on pathological conditions, with relatively few studies involving healthy women. To gain comprehensive insights into the vaginal mycobiota of Algerian women in two different age groups, we performed a targeted metagenomic analysis using ITS2 region sequencing data from 14 vaginal samples collected from healthy women in reproductive and postmenopausal stages. A single dominant fungal species per individual was observed in both young and postmenopausal women, with differences in fungal community composition between the two groups being related to hormone levels. Our results show that Candida and Saccharomyces were the dominant genera in both young and postmenopausal women. Notably, the postmenopausal group had twice as many species, along with the presence of uncommon taxa such as Dipodascus and Fusarium, indicating greater taxonomic diversity. These findings suggest that menopause is associated with increased microbial variability, likely due to hormonal changes that disrupt the vaginal environment. This study paves the way for more extensive analyses involving diverse age groups and ethnic backgrounds.</p>","PeriodicalId":9090,"journal":{"name":"Brazilian Journal of Microbiology","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143596219","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}