{"title":"Development of protective egg yolk immunoglobulins (IgY) targeting CfaB, LTB, and EtpA recombinant proteins of Enterotoxigenic Escherichia coli (ETEC) for inhibiting toxin activity and bacterial adherence.","authors":"Maryam Mafi, Razieh Rezaei Adriani, Fatemeh Mohammadkhani, Seyed Latif Mousavi Gargari","doi":"10.1007/s42770-024-01554-0","DOIUrl":"https://doi.org/10.1007/s42770-024-01554-0","url":null,"abstract":"<p><p>Enterotoxigenic Escherichia coli (ETEC) stands as a prevalent bacterial cause of global diarrheal incidents. ETEC's primary virulence factors encompass the B subunit of the Heat Labile Enterotoxin, along with the adhesion factors CfaB and EtpA. In this study, we isolated IgY antibodies against the three virulence factors individually, in pairs, and as triple cocktails. The in vitro efficacy of these IgY antibodies was examined, focusing on inhibiting heat-labile enterotoxin (LT) toxin cytotoxicity and impeding ETEC adherence to HT29 cells. Assessing the impact of IgY-treated bacteria on intestinal epithelial cells utilized the standard ileal loop method. Results demonstrated that the anti-LTB IgY antibody at 125 µg/ml and IgY antibodies from double and tertiary cocktails at 200 µg/ml effectively inhibited LT toxin attachment to the Y1 cell line. Pre-incubation of HT29 intestinal cells with specific IgYs reduced bacterial attachment by 59.7%. In the ileal loop test, toxin neutralization with specific IgYs curtailed the toxin's function in the intestine, leading to a 74.8% reduction in fluid accumulation compared to control loops. These findings suggest that egg yolk immunoglobulins against recombinant proteins LTB, CfaB, and EtpA, either individually or in combination, hold promise as prophylactic antibodies to impede the functioning of ETEC bacteria.</p>","PeriodicalId":9090,"journal":{"name":"Brazilian Journal of Microbiology","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2024-11-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142581838","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Clinical, epidemiological, and molecular characteristics of SARS-CoV-2 Infections among healthcare workers at a research center in the amazon region of BRAZIL from 2020 to 2022.","authors":"Darciane Coelho Cordovil, Delana Andreza Melo Bezerra, Rayssa Layna Silva Bedran, Edvaldo Tavares Penha Junior, Dielle Monteiro Teixeira, Patricia Santos Lobo, Jones Anderson Monteiro Siqueira, Adinaura Gama Ramos, Amanda Mendes Silva, Kenny Costa Pinheiro, Jedson Cardoso Ferreira, Wanderley Dias Chagas Junior, Luana Soares Barbagelata, Fernando Neto Tavares, Mirleide Cordeiro Santos, Luana Silva Soares","doi":"10.1007/s42770-024-01557-x","DOIUrl":"https://doi.org/10.1007/s42770-024-01557-x","url":null,"abstract":"<p><p>The coronavirus disease-2019 (COVID-19) pandemic has affected different sectors of society, and healthcare workers have been particularly impacted. This study aimed to describe the clinical, epidemiological, and molecular characteristics of SARS-CoV-2 infections among healthcare workers in Evandro Chagas Institute, a research reference center in Brazil, from October 2020 to July 2022. 845 samples were collected from individuals who presented clinical symptoms of respiratory infection. Nasopharyngeal positive samples were submitted through genome sequencing. Clinical, epidemiological, and the SARS-CoV-2 lineages (or variants) were analyzed. SARS-CoV-2 positivity was detected in 31.8% (269/845) of samples with a higher prevalence of females (60.2%). The highest SARS-CoV-2 positivity rates were reported in March 2021 (39%), January 2022 (65%), and July 2022 (56%). On clinical symptoms, arthralgia, chills, and diarrhea were statistically significantly detected in 2020; fever, runny nose, and arthralgia in 2021; runny nose, and cough in 2022. On molecular analysis of SARS-CoV-2, 66 samples (25.3%, 66/269) were sequenced and the most prevalent lineage was the Omicron, representing 57.6%. Studies on the epidemiological and clinical characteristics of HCW are essential to propose control measures and work management since research centers play a major role in surveillance to identify and monitor infectious diseases.</p>","PeriodicalId":9090,"journal":{"name":"Brazilian Journal of Microbiology","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142562622","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Márcio Garcia Ribeiro, Ana Beatriz da Silva Ribeiro, Ana Beatriz Matias da Silva, Gabriel Henrique Gomes Mariano, Larissa de Sá Teles Bertunes, Fábio Vinicius Ramos Portilho, Marcelo Fagali Arabe Filho, Thaís Spessotto Bello, Júlia Meira, Patrik Júnior de Lima Paz, Amanda Keller Siqueira, Rodrigo Garcia Motta, Lorrayne de Souza Araújo Martins Motta, Amanda Bezerra Bertolini, Rogério Giuffrida, Aline Garcia Casteleti, Fernando José Paganini Listoni, Antonio Carlos Paes
{"title":"Peritonitis-related bacterial infections: a large-scale case-series retrospective study in 160 domestic animals (2009-2022).","authors":"Márcio Garcia Ribeiro, Ana Beatriz da Silva Ribeiro, Ana Beatriz Matias da Silva, Gabriel Henrique Gomes Mariano, Larissa de Sá Teles Bertunes, Fábio Vinicius Ramos Portilho, Marcelo Fagali Arabe Filho, Thaís Spessotto Bello, Júlia Meira, Patrik Júnior de Lima Paz, Amanda Keller Siqueira, Rodrigo Garcia Motta, Lorrayne de Souza Araújo Martins Motta, Amanda Bezerra Bertolini, Rogério Giuffrida, Aline Garcia Casteleti, Fernando José Paganini Listoni, Antonio Carlos Paes","doi":"10.1007/s42770-024-01551-3","DOIUrl":"https://doi.org/10.1007/s42770-024-01551-3","url":null,"abstract":"<p><p>Bacterial peritonitis infections comprise a life-threatening clinical condition in domestic animals that commonly lead to sepsis and high mortality. A set of bacterial pathogens have been identified in septic peritonitis in livestock and companion animals. Nonetheless, most descriptions are restricted to case reports or limited to only one domestic species, and a restrict number of comprehensive studies involving this infection has focused on a great number of domestic animals. Here, we retrospectively investigated selected epidemiological data (with an emphasis in outcome), clinical signs, bacteriological culturing, and in vitro antimicrobial susceptibility patterns of microorganisms isolated of peritoneal fluid from 160 domestic animals (2009-2023) compatible with septic peritonitis. Bacteria were isolated from 71.9% (115/160) of the peritoneal fluid from 75 dogs (75/115 = 65.2%), 22 cats (22/115 = 19.1%), 14 horses (14/115 = 12.2%), and 4 cattle (4/115 = 3.5%). Among animals with bacterial isolation, Escherichia coli (34/115 = 29.6%), alfa-hemolytic Streptococcus (12/115 = 10.4%), Staphylococcus aureus (8/115 = 6.9%), beta-hemolytic Streptococcus (7/115 = 6.1%), and Pasteurella multocida (6/115 = 5.2%) were predominant in pure culture, in addition to a miscellaneous of other bacteria isolated in minor frequency, e.g., Pseudomonas sp., Trueperella pyogenes, Klebsiella pneumoniae, and Salmonella sp. In general, in vitro susceptibility tests of isolates revealed that florfenicol, chloramphenicol, and amoxicillin/clavulanic acid showed moderate effectivity (≥ 60%). Conversely, most of isolates exhibited resistance mainly to trimethoprim/sulfamethoxazole, enrofloxacin, and penicillin (> 60%). Additionally, multidrug resistance was found in 42.6% (49/115) of the isolates. Data related to the outcome were available in 37.4% (43/115) of animals that had bacterial isolation and, from these, the mortality rate was 79.1% (34/43), with a significant association (p < 0.036) between mortality and septic peritonitis by gram-negative bacteria. Neoplasia (7/43 = 16.3%), pneumonia/pulmonary abscess (5/43 = 11.6%), hepatitis (5/43 = 11.6%), metritis/pyometra (4/43 = 9.3%), and gall bladder rupture (3/43 = 7%) represented the probable main sources of septic peritonitis. Anorexia (34/115 = 29.6%), emesis (29/115 = 25.2%), lethargy (26/115 = 22.6%), respiratory distress (25/115 = 21.7%), ascites (20/115 = 17.4%), and fever (19/115 = 16.5%) were the most frequent clinical signs among animals with bacterial isolation. A variety of bacteria were isolated in the peritoneal fluid of animals, with a predominance of Enterobacteriaceae, streptococci, and staphylococci, highlighting the opportunistic nature of the pathogens in septic peritonitis. High in vitro multidrug resistance of isolates and high mortality of animals reinforce the need for early diagnosis and therapy based on the in vitro antimicrobial profile of the pathogens involved in septic peritonitis. Our ","PeriodicalId":9090,"journal":{"name":"Brazilian Journal of Microbiology","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2024-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142543577","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Santiago M Lattar, Rene Peter Schneider, Vidal Jorge Eugenio, Gabriel Padilla
{"title":"High release of Candida albicans eDNA as protection for the scaffolding of polymicrobial biofilm formed with Staphylococcus aureus and Streptococcus mutans against the enzymatic activity of DNase I.","authors":"Santiago M Lattar, Rene Peter Schneider, Vidal Jorge Eugenio, Gabriel Padilla","doi":"10.1007/s42770-024-01550-4","DOIUrl":"10.1007/s42770-024-01550-4","url":null,"abstract":"<p><p>This study aimed to determine the protective role of the high release of C. albicans extracellular DNA (eDNA) in a polymicrobial biofilm formed by S. aureus and S. mutans in the course of DNase I treatment. A tube-flow biofilm bioreactor was developed to mimic biofilm formation in the oral cavity. eDNA release was quantified by real-time PCR (qPCR) and confocal microscopy analysis were used to determine the concentration and distribution of eDNA and intracellular DNA (iDNA). The mean amount of eDNA released by each species in the polymicrobial was higher than that in monospecies biofilms (S. aureus: 3.1 × 10<sup>-2</sup> ng/μl polymicrobial versus 5.1 × 10<sup>-4</sup> ng/μl monospecies; S. mutans: 3 × 10<sup>-1</sup> ng/μl polymicrobial versus 2.97 × 10<sup>-2</sup> ng/μl monospecies; C. albicans: 8.35 ng/μl polymicrobial versus 4.85 ng/μl monospecies). The large amounts of eDNA released by C. albicans (96%) in polymicrobial biofilms protects the S. aureus and S. mutans cells against the degradation by DNase I and dampens the effect of clindamycin.</p>","PeriodicalId":9090,"journal":{"name":"Brazilian Journal of Microbiology","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2024-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142557123","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Anti-quorum sensing activity of essential oils against multidrug-resistant Klebsiella pneumoniae: a novel approach to control bacterial virulence.","authors":"Akshatha Naik, Ramya Premanath","doi":"10.1007/s42770-024-01546-0","DOIUrl":"https://doi.org/10.1007/s42770-024-01546-0","url":null,"abstract":"<p><p>The increasing resistance of microbes to conventional drugs is a serious problem worldwide that has increased the need for alternative antimicrobial compounds. Naturally occurring essential oils (EOs) are considered an important component of traditional pharmacopeia because of their antimicrobial and antioxidant properties. This has attracted researchers to identify novel therapeutic anti-pathogenic agents that could act as non-toxic quorum sensing inhibitors, thus controlling infections without encouraging the development of bacterial resistance. This prompted to undertake the current investigation to unravel the efficacy of EOs as QS modulators in reducing the virulence of multidrug resistant (MDR) strains of Klebsiella pneumoniae. The study highlighted the anti-QS activity of fifteen EOs in modulating the QS-related traits by a reduction in capsular polysaccharide, exopolysaccharide and siderophore production in addition to inhibition of biofilm formation. The overall results suggest using EOs to develop alternate intervention strategies to mitigate infections caused by MDR strains of K. pneumoniae.</p>","PeriodicalId":9090,"journal":{"name":"Brazilian Journal of Microbiology","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2024-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142543575","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Osvaldo Manuel Núñez Nogueira, Suzan Prado Fernandes Bernal, Cleto Kaveski Peres, Marcela Boroski, Michel Rodrigo Zambrano Passarini
{"title":"Isolation of marine-derived filamentous fungi and their potential application for bioremediation process.","authors":"Osvaldo Manuel Núñez Nogueira, Suzan Prado Fernandes Bernal, Cleto Kaveski Peres, Marcela Boroski, Michel Rodrigo Zambrano Passarini","doi":"10.1007/s42770-024-01536-2","DOIUrl":"https://doi.org/10.1007/s42770-024-01536-2","url":null,"abstract":"<p><p>We evaluated the bioremediation potential of petroleum-derived compounds using fungal strains isolated from marine samples collected on the coast of the states of Paraná, Brazil. About 75 isolated filamentous fungi were subjected to assays including decolorization of the synthetic dye Remazol Brilliant Blue R (RBBR), tolerance to diesel oil, production of bioemulsifying and degradation of pyrene. Nine isolates could decolorize RBBR between 3.4% and 88.16%. Ten were able to tolerate diesel oil and/or pyrene. One isolate was able to produce compounds with emulsifying properties. Three strains, Trichoderma sp. FM14 (Penicillium spp. FM02 and FM16, and FM14) were able to degrade pyrene between 33.0 and 42.4%, after 8 days. The results of the present work encourage future studies to optimize enzymatic conditions using isolates with biotechnological potential in bioremediation studies of marine environments contaminated with industrial pollutants including hydrocarbons derived from petroleum such as diesel oil and PAHs and synthetic dyes.</p>","PeriodicalId":9090,"journal":{"name":"Brazilian Journal of Microbiology","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2024-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142543576","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ilana Nascimento de Sousa Santos, Walter Lilenbaum, Daniela Sales Alviano Moreno, Maria do Carmo de Freire Bastos
{"title":"Production of bacteriocin-like inhibitory substance (BLIS) by Staphylococcus spp. isolates from dogs.","authors":"Ilana Nascimento de Sousa Santos, Walter Lilenbaum, Daniela Sales Alviano Moreno, Maria do Carmo de Freire Bastos","doi":"10.1007/s42770-024-01532-6","DOIUrl":"https://doi.org/10.1007/s42770-024-01532-6","url":null,"abstract":"<p><p>In the present study, 39 canine isolates of Staphylococcus spp. were tested for antimicrobial substance (AMS) production. Seven AMS producers were identified, whose products exhibited a non-acidic character and a proteinaceous nature, therefore being considered bacteriocin-like inhibitory substances (BLIS). The producer strains of BLIS P1, P16 and I3 showed a broad spectrum of antimicrobial activity. Human, veterinary and plant pathogens, such as Listeria monocytogenes, Klebsiella pneumoniae, Staphylococcus spp. and Clavibacter michiganensis, were among the inhibited micro-organisms, suggesting the potential biotechnological application of these peptides. MALDI-TOF mass spectrometry and 16 S rDNA sequencing identified the producer strains of BLIS P1, P16 and I3 as Staphylococcus pseudintermedius P1, Staphylococcus schleiferi P16 and Staphylococcus pseudintermedius I3. The plasmid profile of these strains suggests that the BLIS production is linked to biosynthetic genes located on plasmids. PCR analyses revealed that BLIS P1, P16 and I3 are different from 11 staphylococcins already described in the literature and that their genomic DNAs do not carry the most prevalent staphylococcal enterotoxin genes. The highest levels of BLIS production were achieved after 18-24 h of growth of the producer strains in TSB medium. Moreover, BLIS P1 and I3 exhibited high resistance to temperature and pH variations, and BLIS P16 maintained 100% of its activity in almost all conditions tested. The characteristics associated with BLIS P1, P16 and I3 described in this work encourage further investigation of these substances, in addition to this study being the first report of BLIS production by a strain of S. schleiferi.</p>","PeriodicalId":9090,"journal":{"name":"Brazilian Journal of Microbiology","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2024-10-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142521019","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Anderson X B Velasquez, Giselle B Klautau, Mariana Neri L Kurihara, Ingrid Nayara M Santos, Laura B Campos, Mayara Muniz Silva, Icaro S Oliveira, Thomas Stravinskas Durigon, Lais S Seriacopi, Mauro J Salles
{"title":"Improving the microbiological diagnosis of fracture-related infection and prosthetic joint infection through culturing sonication fluid in Bactec blood culture bottles.","authors":"Anderson X B Velasquez, Giselle B Klautau, Mariana Neri L Kurihara, Ingrid Nayara M Santos, Laura B Campos, Mayara Muniz Silva, Icaro S Oliveira, Thomas Stravinskas Durigon, Lais S Seriacopi, Mauro J Salles","doi":"10.1007/s42770-024-01545-1","DOIUrl":"https://doi.org/10.1007/s42770-024-01545-1","url":null,"abstract":"<p><strong>Background: </strong>Sonication of surgically removed implants appears to optimize the microbiological diagnosis in orthopedic implant-associated infections (OIAI). However, reports of infection with negative cultures can still reach high rates. A study evaluating the inoculation of sonication fluid into blood culture bottles (SFBCB) in patients with fracture-related infection (FRI) and prosthetic joint infection (PJI) is necessary. This study compared the accuracy SFBCB over the conventional sonication fluid cultures (CSFC) and tissue culture (TC).</p><p><strong>Methods: </strong>Consecutive patients who underwent implant removal surgeries due to any reason had their implants sonicated according to standardized method. Definitions of PJI and FRI were based upon criteria by European Bone and Joint Infection Society (EBJIS) and Metsemakers, respectively. Between three to five intraoperative tissue samples were processed. The implant`s sonication fluid was seeded onto sheep blood agar, chocolate agar, thioglycolate broth and on tryptic soy broth for CSFC, while was also inoculated into blood culture bottles and incubated in the automated system during 5 days for SFBCB.</p><p><strong>Results: </strong>Overall, 74 patients were analyzed, of which 57 with OIAI (48 FRI and 09 PJI) and 17 aseptic failures (03 arthroplasties and 14 osteosynthesis). Interestingly, SFBCB demonstrated significantly higher sensitivity compared to CSFC (96.5% [95% CI, 88-100] vs. 78.9% [95% CI, 66-89], p = 0.004), and to TC (96.5% [95% CI, 88-100], vs. 57.9% [95% CI, 44-71], p < 0.001), whereas there were no significant differences in specificity between the three methods.</p><p><strong>Conclusion: </strong>In comparison to CSFC and TC, SFBCB improved sensitivity for diagnosing OIAI without compromising specificity.</p>","PeriodicalId":9090,"journal":{"name":"Brazilian Journal of Microbiology","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2024-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142521016","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Emad Al-Ebshahy, Mohammed AboElkhair, Awad Shehata, Emad Elgendy
{"title":"First report on genetic characterization of egg drop syndrome 1976 virus in Egypt.","authors":"Emad Al-Ebshahy, Mohammed AboElkhair, Awad Shehata, Emad Elgendy","doi":"10.1007/s42770-024-01537-1","DOIUrl":"https://doi.org/10.1007/s42770-024-01537-1","url":null,"abstract":"<p><p>Since its first description in 1991 in Egypt, egg drop syndrome 1976 (EDS-76) virus has received a little attention as a potential cause for the drop in egg production as well as the reduction in egg quality. To date, no studies have been carried out to describe the genetic characteristics of the circulating field EDS-76 virus strains. Thus, the present study was attempted to estimate the emergence of EDS-76 virus in layer flocks and to determine the genetic diversity between the field strains and the vaccine strain 127. During 2022, a total of 5 apparently healthy backyard layer flocks were investigated for the presence of EDS-76 virus infection following complaints of sudden drop in egg production (25-30%), accompanied by high incidence of eggshell defects. EDS-76 virus DNA was detected in the oviduct samples of 4 (80%) flocks by polymerase chain reaction (PCR) assay targeting the hexon gene of the viral capsid. Attempts of viral isolation in duck embryo revealed no embryonic mortality, however, the allantoic fluids of inoculated eggs exhibited a sustained increase in the hemagglutinating (HA) activity throughout three consecutive passages. The obtained strain, designated BH-1, was characterized on the basis of partial hexon gene sequence analysis (GenBank accession number OR531368). The BH-1 strain displayed 99.6% nucleotide identity with the vaccine strain 127. However, amino acid alignments with the vaccine strain 127 revealed that the BH-1 strain carried 5 non-synonymous mutations. In addition, two of these mutations were incorporated into the hexon hypervariable regions (HVRs), which are strictly responsible for eliciting serotype-specific neutralizing antibodies. In conclusion, the present study represents a starting point for genetic characterization of EDS-76 virus in Egypt and highlights the importance for continuous monitoring and characterization of the circulating field EDS-76 virus strains, in order to determine the proper control strategy.</p>","PeriodicalId":9090,"journal":{"name":"Brazilian Journal of Microbiology","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2024-10-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142494964","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Jéssica Daniel Martins da Silva, Lucas Borges Pereira, Fabiana Rossi Varallo, Mariana Martins Gonzaga do Nascimento, Larissa Helena Torres, Paulo Roberto Maia, Carla Speroni Ceron, Tiago Marques Dos Reis
{"title":"Evaluation of the perspective of pharmacists on antimicrobial stewardship and the barriers to its consolidation in hospitals: a cross-sectional study.","authors":"Jéssica Daniel Martins da Silva, Lucas Borges Pereira, Fabiana Rossi Varallo, Mariana Martins Gonzaga do Nascimento, Larissa Helena Torres, Paulo Roberto Maia, Carla Speroni Ceron, Tiago Marques Dos Reis","doi":"10.1007/s42770-024-01540-6","DOIUrl":"https://doi.org/10.1007/s42770-024-01540-6","url":null,"abstract":"<p><p>Antimicrobial Stewardship Programs (ASPs) are associated with positive outcomes, but seem to be consolidated in few hospitals. This study aimed to evaluate ASPs in Brazilian hospitals and the barriers to their consolidation from the pharmacists' perspective. This is a cross-sectional study. Data were collected through an online questionnaire applied to hospital pharmacists. Data analysis was performed by descriptive statistics and Spearman's ordinal correlation test. A total of 83 pharmacists participated in the study and 45.8% stated that the hospital in which they worked had an ASP. The ASPs were predominantly implemented in public hospitals, with a greater number of beds in the intensive care unit and beds in general. The main barriers to the consolidation of ASPs were: reduced number of pharmacists, time allocated to other demands of the pharmacy, the ineffectiveness of the communication process with doctors, and lack of access to reliable information about antimicrobials in the hospital. Pharmacists that work in hospitals where ASPs were implemented were more confident (p = 0.000) and with a better perception of the barriers that prevent their consolidation (p = 0.003. The ASP was commonly adopted in public hospitals with a greater number of beds. The main obstacles to consolidation were infrastructure, economic, and educational resources.</p>","PeriodicalId":9090,"journal":{"name":"Brazilian Journal of Microbiology","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2024-10-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142494963","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}