Brazilian Journal of Microbiology最新文献

{"title":"Unveiling remarkable bacterial diversity trapped by cowpea (Vigna unguiculata) nodules inoculated with soils from indigenous lands in Central-Western Brazil.","authors":"Fernanda Terezinha Moura, Caroline Vanzzo Delai, Milena Serenato Klepa, Renan Augusto Ribeiro, Marco Antonio Nogueira, Mariangela Hungria","doi":"10.1007/s42770-025-01622-z","DOIUrl":"https://doi.org/10.1007/s42770-025-01622-z","url":null,"abstract":"<p><p>Cowpea (Vigna unguiculata) is recognized as a promiscuous legume in its symbiotic relationships with rhizobia, capable of forming associations with a wide range of bacterial species. Our study focused on assessing the diversity of bacterial strains present in cowpea nodules when inoculated with soils from six indigenous lands of Mato Grosso do Sul state, Central-Western Brazil, comprising the Cerrado and the Pantanal biomes, which are known for their rich diversity. The DNA profiles (BOX-PCR) of 89 strains indicated great genetic diversity, with 20 groups and 23 strains occupying single positions, and all strains grouped at a final similarity level of only 25%. Further characterization using 16S rRNA gene sequencing revealed a diverse array of bacterial genera associated with the cowpea nodules. The strains (number in parenthesis) were classified into ten genera: Agrobacterium (47), Ancylobacter (2), Burkholderia (12), Ensifer (1), Enterobacter (1), Mesorhizobium (1), Microbacterium (1), Paraburkholderia (1), Rhizobium (22), and Stenotrophomonas (1), split into four different classes. Notably, only Ensifer, Mesorhizobium, Rhizobium, and Paraburkholderia are classified as rhizobia. Phylogenetic analysis was conducted based on the classes of the identified genera and the type strains of the closest species. Our integrated analyses, combining phenotypic, genotypic, and phylogenetic approaches, highlighted the significant promiscuity of cowpea in associating with a diverse array of bacteria within nodules, showcasing the Brazilian soils as a hotspot of bacterial diversity.</p>","PeriodicalId":9090,"journal":{"name":"Brazilian Journal of Microbiology","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-01-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143022096","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative evaluation of protective efficacy of experimental inactivated vaccines against haemorrhagic septicaemia.","authors":"Pankaj Dhakarwal, Manisha Medhi, Dhanvelu Muthuchelvan, Pallab Chaudhuri, K N Viswas, B H Manjunatha Patel, Sathish Bhadravati Shivachandra, V Bhanuprakash, Muthannan Andavar Ramakrishnan","doi":"10.1007/s42770-024-01610-9","DOIUrl":"https://doi.org/10.1007/s42770-024-01610-9","url":null,"abstract":"<p><p>Developing an effective vaccine for haemorrhagic septicaemia (HS) in cattle and buffaloes is urgently needed. While preferred for their safety, achieving sufficient, cross-protective, and long-lasting immunity is still challenging when administering inactivated vaccines. This study aimed to assess the efficacy of four inactivating components comprising three inactivating agents: (1) Binary ethylenimine (BEI), (2) Formalin, (3) a combination of BEI and Formalin, and (4) Hydrogen peroxide (H₂O₂), in inactivating Pasteurella multocida to enhance HS vaccine potency. H₂O₂ demonstrated the fastest inactivation kinetics, killing Pasteurella multocida within a few minutes. The combination of BEI and Formalin showed relatively rapid inactivation compared to Formalin or BEI alone. BEI, known for targeting nucleic acids with minimal impact on protein integrity, showed promise but required higher concentrations (8 mM) and a longer duration (16 h) for complete inactivation. Based on the inactivation kinetics, 8 mM BEI, 0.1% Formalin, 8 mM BEI + 0.1% Formalin, and 1% H₂O₂ were chosen for the experimental vaccine formulations. The inactivated Pasteurella multocida were adjuvanted with Montanide ISA-201 oil adjuvant and evaluated in mice model. All vaccine formulations elicited protective responses of over 6.5 log₁₀ units, indicating the high potency of the vaccine formulations. Notably, the vaccine prepared with an H₂O₂ inactivating agent elicited protection of > 8 log₁₀ units. Our study concludes that H₂O₂, with its rapid inactivation kinetics and safety profile, presents a promising alternative inactivating agent for HS vaccine development. Future studies should evaluate the protective efficacy of H₂O₂-inactivated vaccines in target animals.</p>","PeriodicalId":9090,"journal":{"name":"Brazilian Journal of Microbiology","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142999954","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antifungal susceptibility, clinical findings, and biofilm resistance of Fusarium species causing keratitis: a challenge for disease control.","authors":"Pedro de Freitas Santos Manzi de Souza, Edlâny Pinho Romão Milanez, Ana Raquel Colares de Andrade, Lua Silva, Maria Laína Silva, Ruan Costa Monteiro, Anderson Messias Rodrigues, Débora Castelo Branco de Souza Collares Maia, Glaucia Morgana de Melo Guedes, Rossana de Aguiar Cordeiro","doi":"10.1007/s42770-024-01611-8","DOIUrl":"https://doi.org/10.1007/s42770-024-01611-8","url":null,"abstract":"<p><p>Fusarium keratitis (FK) is an important clinical condition that can lead to blindness and eye loss, and is most commonly caused by the Fusarium solani species complex (FSSC). This study evaluated the susceptibility of planktonic cells and biofilms of FSSC (n = 7) and non-FSSC (n = 7) isolates obtained from patients with keratitis from a semi-arid tropical region to amphotericin B (AMB), natamycin (NAT), voriconazole (VRZ), efinaconazole (EFZ), and luliconazole (LCZ). Analysis of clinical data showed that trauma was the most common risk factor for FK patients. Disease onset was longer in non-FSSC group (3-30 days) than in the FSSC group (3-7 days). FSSC strains were less susceptible to AMB and VRZ than non-FSSC strains (p < 0.05). Susceptibility to NAT, LCZ and EFZ was similar between isolates of FSSC and non-FSSC groups. Overall, patients infected with non-FSSC showed a better response to antifungal treatment. Corneal transplantation was more common in patients infected with FSSC (3/7) than in those infected with non-FSSC (1/7). Mature biofilms showed a poor response to antifungal treatment. Patients infected with Fusarium strains capable of forming antifungal tolerant biofilms had more complex therapeutic management, requiring two antifungals and/or corneal transplantation (p < 0.05). This study highlights the importance of mycological diagnosis and the antifungal susceptibility testing in the clinical management of FK. The ability of Fusarium to form antifungal tolerant biofilms poses a challenge to clinicians and urges the development of new antibiofilm therapeutics.</p>","PeriodicalId":9090,"journal":{"name":"Brazilian Journal of Microbiology","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-01-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142999953","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Carbon-dot pequi-nut in the development of immunosensor to detect pathogenic bacteria.","authors":"Rachel Menezes Castelo, Marília de Albuquerque Oliveira, Roselayne Ferro Furtado, Bruno Peixoto de Oliveira, Lucas Vinicius Leite Martoni, Terezinha Feitosa Machado, Celli Rodrigues Muniz, Flávia Oliveira Monteiro da Silva Abreu, Sérgio Antônio Spinola Machado, Airis Maria Araújo Melo, Huai N Cheng, Atanu Biswas, Carlucio Roberto Alves","doi":"10.1007/s42770-025-01612-1","DOIUrl":"https://doi.org/10.1007/s42770-025-01612-1","url":null,"abstract":"<p><p>Carbon dots in biosensing have advanced significantly, adding improvements to different detection techniques. In this study, an amperometric immunosensor for Salmonella Thyphimurium was designed using antibodies labeled with carbon dots (Cdots) from pequi almond (Caryocar brasiliensis). Cdots were synthesized by pyrolysis and characterized by FTIR, UV/fluorescence, electrochemistry, zeta potential, and transmission electron microscopy (TEM). A particle size of 6.80 ± 2.13 nm was estimated, and the zeta potential was - 47.4 mV, indicating the preponderant presence of acidic groups, as confirmed by FTIR. The impedance evaluation of the response of biosensors assembled for live (Rct = 13.4 kΩ) and dead (Rct = 499.7 Ω) Salmonella showed a significant difference in their values, in agreement with chronoamperometric analyses, which had their current values drastically reduced from - 2.2 mA (live) to 0 mA (dead). An analytical curve for Salmonella was established with the limit of detection lower than 1 CFU/mL. This electrochemical biosensor using pequi carbon dots for antibody labeling showed promising results for detecting the pathogen. Thus, carbon dots can be used as substitutes for enzymes in labeling antibodies used in the design and production of sensors.</p>","PeriodicalId":9090,"journal":{"name":"Brazilian Journal of Microbiology","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142982756","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Decontamination of DNA sequences from a Streptomyces genome for optimal genome mining.","authors":"Raul Vitor Ferreira de Oliveira, Leandro Maza Garrido, Gabriel Padilla","doi":"10.1007/s42770-024-01598-2","DOIUrl":"https://doi.org/10.1007/s42770-024-01598-2","url":null,"abstract":"<p><p>Despite meticulous precautions, contamination of genomic DNA samples is not uncommon, which can significantly compromise the analysis of microorganisms' whole-genome sequencing data, thus affecting all subsequent analyses. Thanks to advancements in software and bioinformatics techniques, it is now possible to address this issue and prevent the loss of the entire dataset obtained in a contaminated whole-genome sequencing, where the DNA of another bacterium is present. In this study, it was observed that the sequencing reads from Streptomyces sp. BRB040, generated using the HiSeq System platform (Illumina Inc., San Diego, USA), were contaminated with the DNA of Bacillus licheniformis. To eliminate the contamination in Streptomyces sp. BRB040, a combination of tools available on the Galaxy platform and other web-based resources were used (MeDuSa and Blast). The contaminated reads were treated as a metagenome to isolate the genome of the contaminating organism. They were assembled using the metaSPAdes, resulting in a large scaffold of 4.187 Mb, which was identified as Bacillus licheniformis. After the identification of the contaminating organism, its genome was used as a filter to remove sequencing reads that could align using then Bowtie 2 software for this step. Once the contaminated reads were removed a new assembly was performed using the Unicycler software, yielding 117 contigs with a total size of 7.9 Mb. The completeness of this genome was assessed through BUSCO, resulting in a completeness of 95.9%. We also used an alternative tool (BBduk) to eliminate contaminated reads and the resulting assembly by Unicycler generated 85 contigs with a total size of 8.3 Mb and completeness of 99.5%. These results were better than the assembly obtained via SPAdes, which generated less complete genomes (maximum of 97.8% completeness) compared to Unicycler and which was unable to perform an adequate assembly of the data obtained from decontamination by BBduk. When compared with the uncontaminated BRB040 genome, which has a total size of 8.2 Mb and completeness of 99.8%, this pipeline revealed that the assembly performed with the decontaminated reads via BBduk presented better results, with completeness 0.3% lower than the reference. The genome mining of both genomes using antiSMASH 7.0 revealed the number of 24 Biosynthetic Gene Clusters (BGCs) for BBduk data as well as in the control assembly of the BRB040. In silico decontamination process allows the genome mining of BGCs despite the loss of nucleotides. These findings show that contamination can be effectively removed from a genome using readily available online tools, while preserving a dataset suitable for extracting valuable insights into the secondary metabolism of the target organism. This approach is particularly beneficial in scenarios where resequencing samples is not immediately feasible.</p>","PeriodicalId":9090,"journal":{"name":"Brazilian Journal of Microbiology","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142982762","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microbiological and toxicity analyses of the synthetic polymer polyhexamethylene guanidine hydrochloride against endodontic microorganisms.","authors":"Laila Dainize Finotelli, Carlos Henrique Gomes Martins, Sara Lemes de Souza, Anna Livia Oliveira Santos, Mariana Brentini Santiago, Sérgio Ricardo Ambrósio, Rodrigo Cássio Sola Veneziani, Renato Luis Tame Parreira, Leandro Aparecido Mello, Lucas de Freitas Pereira, Fernanda Gosuen Gonçalves Dias","doi":"10.1007/s42770-024-01603-8","DOIUrl":"https://doi.org/10.1007/s42770-024-01603-8","url":null,"abstract":"<p><p>Failures in endodontic treatments are common due to microbial resistance in the pulp canal. The study evaluated the in vitro activity of polyhexamethylene guanidine hydrochloride (PHMGH) against endodontic strains, as well as in vivo toxicity. Using minimum inhibitory concentration and minimum bactericidal concentration techniques, PHMGH was effective against all microorganisms, even at low concentrations. At 50.0 µg/mL, it inhibited Enterococcus faecalis; furthermore, when compared to chlorhexidine (CLX), it demonstrated values 19 times lower against Candida albicans. The polymer's activity was also determined by agar diffusion, evaluating products A (calcium hydroxide - Ca(OH)2, as a reference), B (Ca(OH)2 combined with physiological solution, reference with a vehicle), C (PHMGH 6.25%), D (PHMGH 3.125%), E (PHMGH 1.5625%), F (PHMGH 0.78125%), G (PHMGH 6.25% and Ca(OH)2), H (PHMGH 3.125% and Ca(OH)2), I (PHMGH 1.5625% and Ca(OH)2), J (PHMGH 0.78125% and Ca(OH)2), and K (positive control, CLX 0.12%). Products containing PHMGH were more effective than the references against all strains, and C, D, and G were more effective than CLX against Peptostreptococcus anaerobius, Actinomyces naeslundii, and Actinomyces viscosus. According to the fractional inhibitory concentration index, the combination of PHMGH and CLX showed indifference for Peptostreptococcus anaerobius, Actinomyces naeslundii, Actinomyces viscosus and Escherichia coli, antagonism for Candida albicans, and synergy for Enterococcus faecalis. The toxicity of PHMGH at different concentrations was tested in Caenorhabditis elegans and did not show lethality in nematodes, with the LC50 observed only at the highest concentration (100 µg/mL) after two days of exposure. It is suggested that PHMGH exhibited antimicrobial activity against endodontic strains and low toxicity, raising expectations for new preventive and therapeutic products in endodontics.</p>","PeriodicalId":9090,"journal":{"name":"Brazilian Journal of Microbiology","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142982834","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Virulent and multidrug-resistant Aeromonas in aquatic environments of Kerala, India: potential risks to fish and humans.","authors":"Vandan Nagar, Farhat Ansari, Murugadas Vaiyapuri, Toms C Joseph","doi":"10.1007/s42770-024-01601-w","DOIUrl":"https://doi.org/10.1007/s42770-024-01601-w","url":null,"abstract":"<p><p>Aeromonas inhabit diverse aquatic habitats and are recognized as both opportunistic and primary pathogens of fish and humans. This study delineates the biochemical and gyrB sequence-based molecular identification of 14 Aeromonas strains isolated from aquatic environments in Kerala, India, identifying them as A. dhakensis (50%), A. hydrophila (28.6%), and A. jandaei (21.4%). These strains exhibit a high prevalence of virulence genes (act, flaA, ser, gcat, lip, and ela) implicated in pathogenesis in both fish and humans. These findings underline the emergence of A. dhakensis, often misidentified as A. hydrophila, as a potential pathogen, highlighting the necessity for comprehensive identification methods. Significantly, all strains demonstrated beta-hemolysis and moderate to strong biofilm formation, enhancing their infectivity potential. Moreover, all isolates exhibited multidrug resistance, with a multiple antimicrobial resistance (MAR) index ranging from 0.39 to 0.56, and a significant presence of class 1 (500-1100 bp) and class 2 (250-700 bp) integrons, indicating their potential risk to both fish and human populations. Our results underscore the role of aquatic environment as a repository for virulent and multidrug-resistant Aeromonas spp., emphasizing the imperative for prudent antimicrobial usage and regular monitoring of antimicrobial resistance (AMR) in these environments.</p>","PeriodicalId":9090,"journal":{"name":"Brazilian Journal of Microbiology","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-01-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142976887","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cecal microbial composition and serum concentration of short-chain fatty acids in laying hens fed different fiber sources.","authors":"Lorena Salim de Sousa, Dayse Helena Lages da Silva, Alexandre Rodrigues Cardoso, Larissa Gonçalves Moreira, Diego Lisboa Rios, Roselene Ecco, Itallo Conrado Sousa Araújo, Leonardo José Camargos Lara","doi":"10.1007/s42770-024-01606-5","DOIUrl":"https://doi.org/10.1007/s42770-024-01606-5","url":null,"abstract":"<p><p>The intestinal microbiota is widely recognized as an integral factor in host health, metabolism, and immunity. In this study, the impact of dietary fiber sources on the intestinal microbiota and the production of short-chain fatty acids (SCFAs) was evaluated in Lohmann White laying hens. The hens were divided into four treatment groups: a control diet without fiber, a diet with wheat bran (mixed fibers), a diet with insoluble fiber (cellulose), and a diet with soluble fiber (pectin), with six replicates of four hens each. Cecal content from 24 hens was analyzed using 16 S rRNA sequencing, while SCFA concentrations were measured in blood serum. Alpha diversity analysis revealed significant variations in microbial richness and diversity among treatments, with higher species richness observed in hens fed wheat bran and cellulose, as indicated by Shannon indices. Principal Coordinates Analysis (PCoA) showed significant differences in microbial composition between the control group and the fiber-supplemented groups. The predominant phyla were Bacteroidetes, Campilobacterota, Firmicutes, and Spirochaetota, with a notable increase in Bacteroidetes in fiber-supplemented groups. Regarding SCFAs, fiber inclusion increased acetic and propionic acid concentrations compared to the control group. Diets with mixed fibers (wheat bran) resulted in the highest acetic acid levels, while propionic acid was most abundant in hens fed soluble fiber (pectin). These findings demonstrate that dietary fiber inclusion to laying hens enhances microbial diversity, stimulates SCFA production, and contributes to host metabolism and health.</p>","PeriodicalId":9090,"journal":{"name":"Brazilian Journal of Microbiology","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-01-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142969563","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Presence of polyketide synthases and nonribosomal peptide synthetase in culturable bacteria associated with Aplysina fulva and Aplysina caissara (Porifera).","authors":"Letícia Sanfilippo Rojas, Michelle Guzmán de Fernandes, Andressa Cristina Ramaglia da Motta, Luiza Porteiro Carminato, Ana Caroline Zanatta, Gisele Lôbo-Hajdu, Paula Jimenez, Márcio Reis Custódio, Cristiane Cassiolato Pires Hardoim","doi":"10.1007/s42770-024-01588-4","DOIUrl":"https://doi.org/10.1007/s42770-024-01588-4","url":null,"abstract":"<p><p>Culture-dependent and -independent studies have provided access to symbiont genes and the functions they play for host sponges. Thus, this work investigates the diversity, presence of genes of pharmacological interest, biological activities and metabolome of the bacteria isolated from the sponges Aplysina caissara and Aplysina fulva collected on the southwestern Atlantic Coast. The genes for Polyketide Synthases types I and II and Nonribosomal Peptide Synthetases were screened in more than 200 bacterial strains obtained, from which around 40% were putatively novel. Twenty-two were positive for at least one of the genes screened. Among them, 12 exhibited antimicrobial activities and one inhibited the proliferation of cancer cells. The metabolic profiles of the 22 strains were analyzed by liquid chromatography with tandem mass spectrometry and molecular network. The Global Natural Products Social Molecular Networking MolNetEnhancer workflow provided a more comprehensive understanding of the metabolic profiles. The results revealed the existence of a wide range of metabolites, however more than half of the compounds could not be identified. It was further observed that the metabolic diversity among the strains varied primarily due to the cultivation medium used. Together the results obtained here revealed the pharmacological potential of the bacteria isolated from Aplysina species.</p>","PeriodicalId":9090,"journal":{"name":"Brazilian Journal of Microbiology","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142944239","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Expanding the evidence for cross-species viral transmission from trophic interactions of parasitoid wasps and their hosts.","authors":"Gabriela B Caldas-Garcia, Ícaro Santos Lopes, Eric Roberto Guimarães Rocha Aguiar","doi":"10.1007/s42770-024-01596-4","DOIUrl":"https://doi.org/10.1007/s42770-024-01596-4","url":null,"abstract":"<p><p>Parasitoid wasps act as natural biological control agents for several harmful insect species. However, there is a lack of information regarding the exogenous RNA viruses that infect parasitoids and may contribute to the success of their parasitism strategies. This study aimed to investigate the presence, abundance, and replication of known exogenous viruses in two parasitoid wasp species and their corresponding preys. Utilizing publicly available RNA deep-sequencing data, two previously validated viruses from the parasitoid Tetrastichus brontispae were assessed in the target beetles Brontispa longissima and Octodonta nipae from the same geographic region. This study revealed the presence of the iflavirus TbRV-3 in both T. brontispae and O. nipae-derived samples, suggesting a potential exchange of the virus between the parasitoid and its host. In addition, there is substantial evidence that the Halyomorpha halys virus infects the parasitoid Telenomus podisi. Thus, this study proposes a close evolutionary relationship between the HhV strain identified in the parasitoid Telenomus podisi and the original strain detected in the prey H. halys. The viral association between trophically related species, such as parasitoids and their hosts, is demonstrated using features such as abundance and the presence of double-stranded RNA, which serves as a proxy for virus replication. Therefore, RNA viruses may coexist at both trophic levels, conferring an evolutionary advantage to the parasitism strategy.</p>","PeriodicalId":9090,"journal":{"name":"Brazilian Journal of Microbiology","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142944493","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}