Brazilian Journal of Microbiology最新文献

{"title":"Candida albicans migrates itself from the vagina to the uterus and ovaries in estrogenized mice.","authors":"Valeria Mosca, Glaucia Sayuri Arita, Karina Mayumi Sakita, Franciele Abigail Vilugron Rodrigues-Vendramini, Daniella Renata Faria, Pollyanna Cristina Vincenzi Conrado, Camila Barros Galinari, Érika Seki Kioshima, Tania Cristina Alexandrino Becker, Patrícia de Souza Bonfim-Mendonça, Terezinha Inez Estivalet Svidzinski","doi":"10.1007/s42770-024-01602-9","DOIUrl":"https://doi.org/10.1007/s42770-024-01602-9","url":null,"abstract":"<p><p>Vulvovaginal candidiasis (VVC) represents the second cause of vaginal infections in childbearing-age women. It mainly affects the vulva and vagina; however, other organs can be compromised, with consequences that are not well known yet. To evaluate the ability of Candida albicans, inoculated into the vaginal lumen of mice, to migrate to the uterus and ovaries. Female Balb/c mice, 6 weeks old, were estrogenized and infected intravaginally with 1 × 10⁶ CFU/ml of C. albicans for the dual objectives of histopathological evaluation and fungal burden quantification in the vaginal, uterus, and ovary tissues, on days 1, 3, and 5 post-infection. Estrogenized and infected mice presented vaginal exudate and an edematous and erythematous uterus. Vaginal histopathological analysis showed intense desquamation, keratinization, fungal structures, and an inflammatory reaction one day after infection. In the uterus, an inflammatory response and a few fungi from the third day onwards were observed. Similarly, the ovary showed the same changes, but at a lower intensity. A high fungal burden was detected in the vaginal tissue from the first day, reaching nearly 6 log CFU/g on the fifth day. C. albicans inoculated into the vaginal lumen of estrogenized mice was able to invade the vaginal tissue one-day post-infection and was detected in the uterus and ovaries within three days.</p>","PeriodicalId":9090,"journal":{"name":"Brazilian Journal of Microbiology","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142944491","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pathogenic Aspergillus spp. and Candida spp. in coastal waters from southern Brazil: an one health approach.","authors":"Emília Ferreira Andrade, Vanice Rodrigues Poester, Bruna Muradás Esperon, Mariana Rodrigues Trápaga, Jéssica Estefânia Dávila Hidalgo, Fabiana Barreiros Ferreira, Manuel Macedo de Souza, Cecília Bittencourt Severo, Andrea Von Groll, Melissa Orzechowski Xavier","doi":"10.1007/s42770-024-01604-7","DOIUrl":"https://doi.org/10.1007/s42770-024-01604-7","url":null,"abstract":"<p><p>Aspergillus and Candida are ubiquitous fungi included in the group of high priority in the World Health Organization list of fungal pathogens. They are found in various ecosystems and the environmental role in increasing the resistance to antifungals has been shown. Thus, we aimed to determine the occurrence of Aspergillus spp. and Candida spp. pathogenic species in water samples from a coastal ecosystem from southern Brazil, and its antifungal susceptibility profile. Water samples were collected monthly at three environmental sites, over 25 months. Abiotic parameters of the water samples were analyzed as well as antifungal susceptibility. Aspergillus spp. and Candida spp. were detected in 44% (n = 33/75) and 40% (n = 30/75) respectively of the samples, totaling 67 and 96 isolates. Section Fumigati and C. parapsilosis were the most section/species isolated. Triazole resistance was detected in 3% of the Aspergillus spp. (2/67) and in 1% of the Candida spp. (1/96) isolates. Our study contributes with data showing that coastal aquatic environments can serve as a source of infection of resistant fungal isolates, proving the need for environmental surveillance and monitoring of fungal resistance in the One Health perspective.</p>","PeriodicalId":9090,"journal":{"name":"Brazilian Journal of Microbiology","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142943853","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chlamydia in farms located in the Argentine-Brazilian-Paraguay tri-border.","authors":"Ezequiel A Vanderhoeven, Jessica P Mosmann, Adrián Díaz, Cecilia G Cuffini","doi":"10.1007/s42770-024-01586-6","DOIUrl":"https://doi.org/10.1007/s42770-024-01586-6","url":null,"abstract":"<p><p>Chlamydias are zoonotic pathogens, broadly present in several bird species and responsible for economic losses in animal production. Our study focused on assessing the prevalence of Chlamydial species posing zoonotic risks in farm animals within the highly biodiverse Argentine, Brazil, and Paraguay tri-border area, characterized by significant human interaction. We surveyed nine farms in an area and nasally swabbed a total of 62 animals, that included cattle, horses and pigs. DNA was extracted and specific PCR was performed to identify and sequenced chlamydial species. We detected Chlamydia spp. in 6.5% (4/62) of the tested animals, with all positive cases found in cattle. None of the cattle showed symptoms of respiratory disease or had been diagnosed with reproductive disorders. Specific nested PCR confirmed two samples belonged to Chlamydia pecorum and two to Chlamydia psittaci. We report for the first time Chlamydia circulation with zoonotic risk in the region. We propose that surveys in birds and wild mammals could give a better understanding to know what Chlamydial species are circulating in the wild interface. The zoonotic potential should be taking into account as farm workers and the surrounding population could be silent carriers or have respiratory diseases being underdiagnosed, and therefore should be considered in the differential diagnoses.</p>","PeriodicalId":9090,"journal":{"name":"Brazilian Journal of Microbiology","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-01-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142944492","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Globally approved vaccines for COVID-19: a systematic review.","authors":"Brenda de Almeida Perret Magalhães, Jéssica Medeiros Minasi, Rubens Caurio Lobato, Luiza Curi Lemos, Laryssa Saez de Britto, Rhaysa Madruga Barros, Ana Maria Barral de Martínez, Vanusa Pousada da Hora","doi":"10.1007/s42770-024-01600-x","DOIUrl":"https://doi.org/10.1007/s42770-024-01600-x","url":null,"abstract":"<p><p>COVID-19 caused a public health emergency, which instituted a global effort to develop vaccines using different platforms, such as basic types and new-generation vaccines. Considering the importance of vaccination in preventing the severity of infectious diseases and the success in developing and approving vaccines against COVID-19 in record time, it is essential to learn about the characteristics of these vaccines. This study aimed to conduct a structured, systematic review following the PRISMA guideline, to analyze the general characteristics of vaccines approved globally for use against COVID-19. We used the list of approved vaccines available by the WHO as guidance to search for studies in the literature. We searched the terms \"SARS-CoV-2 and vaccine and safety and efficacy\" in the MEDLINE via PUBMED and Web of Science databases. We conducted the research on both bases, including complete articles published from January 2020 to June 2023. The selection of files occurred between May/2021 and June/2023. Therefore, the paper did not consider articles published after this period or vaccines approved after this moment. This study only included approved vaccines; phase three studies published in English. We found 11 published articles from phase three that met the established criteria. The vaccines included in this study were: Cominarty, mRNA-1273 or Spikevax, Vaxzevria or AZD1222 or Covishield, CoronaVac or PicoVacc, and Ad26.COV2.S, SputnikV or Gam-Covid-Vac, Covaxin, NVX-CoV2373 or Covovax or Nuvaxovid, WIV04 and HB02, CoVLP or Covifenz and Convidecia or Ad5-nCoV. We summarized the main findings of each vaccine, considering the vaccine composition, number of doses, efficacy analyses, and main adverse effects. In general, the vaccines had high efficacy rates and few adverse effects. Efficacy values are important for vaccine approval, but they will not necessarily reflect the real-world impact of vaccination. It was seen that the effectiveness of COV2.S, CoronaVac/PicoVacc, Cominarty, and Covaxin vaccines was lower than the efficacy, whereas, for AZD1222/Vaxzevria/Covishield, the two parameters remained at similar rates. All vaccines evaluated have different compositions, dosages, populations, and study designs. All are effective in at least preventing symptomatic COVID-19, causing mild or moderate adverse effects when present.</p>","PeriodicalId":9090,"journal":{"name":"Brazilian Journal of Microbiology","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-01-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142944494","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pioneering study of Egyptian Neem and Jojoba extracts with molecular docking combat hospital multidrug resistant bacteria.","authors":"Toka Khairy, Dina Hatem Amin, Hanaa Mohamed Salama, Iman Mohamed Amin Elkholy, Mostafa Elnakib, Hassan Mahmoud Gebreel, Hayam Abd Elnabi Sayed","doi":"10.1007/s42770-024-01590-w","DOIUrl":"https://doi.org/10.1007/s42770-024-01590-w","url":null,"abstract":"<p><p>Hospital surfaces are often contaminated with multidrug-resistant pathogenic bacteria that cause healthcare-associated infections and lead to increased mortality and morbidity. There is a need for new alternative antibacterial agents to overcome antibiotic resistance. Azadirachta indica and Simmondsia chinensis have been found to possess antibacterial activity and medicinal value. The antibacterial activity of these plant extracts against clinical isolates was investigated using the agar disc diffusion method. These clinical isolates included E. coli, Pseudomonas aeruginosa, Acinetobacter spp., Klebsiella pneumoniae, Stenotrophomonas maltophilia, and methicillin-resistant Staphylococcus aureus (MRSA), which were identified by the vitek-2 system, and resistance genes of selected bacterial strains were identified by using the bioFire FilmArray test. The most potent extract of these plants was the ethanolic extract, where the inhibition percentage of ethanolic Jojoba and Neem extracts was 90.9% and 74.5%, respectively against all the tested pathogens. On the other hand, the methanolic extracts of Neem and Jojoba have different degrees of antibacterial activity against the tested pathogens. The phytochemical components of the most potent extracts (ethanolic extracts) were investigated by gas chromatography‒mass spectrometry (GCMS), which revealed that the ethanolic extracts were enriched in phenolics, flavonoids, and sugars. FTIR analyses of the plant extracts confirmed the presence of alcoholic, carboxylic, and aldehydic moieties. The 2,2-diphenyl-1-picrylhydrazyl (DPPH) activity of the ethanolic extracts of Neem and Jojoba increased in a dose-dependent manner, with average IC50 values of 98.17 ± 0.85, 4.95 ± 0.06, and 4.17 ± 0.04 mg/mL, respectively, for the ethanolic Neem extract, the ethanolic Jojoba extract, and ascorbic acid (standard). Furthermore, increased cytotoxicity was demonstrated in the HFB4 cell line in a dose-dependent manner. The average IC50s of the ethanolic Neem extract and the ethanolic Jojoba extract were 18.18 ± 0.15 and 76.16 ± 1.49 mg/mL, respectively. Moreover, the results for the antibiofilm activity of the ethanolic Neem extract showed that 99.5% of the biofilms formed at 25 mg/ml. In addition, 50 mg/ml of the ethanolic extract of Jojoba had a suppressive effect of 98.2%. The significant components Nonanoic acid (21.9405%) and Palmitic Acid (16.0869%) from Neem and pinitol from Jojoba (82.85%) were selected throughout the molecular docking investigation, by which the chosen constituents inhibited the crystal structure of penicillin-binding protein 4 (PBP4) from Staphylococcus aureus (PDB ID: 1TVF) and the crystal structure of the OXA-48 beta-lactamase (PDB ID: 7AUX) from K. pneumoniae. Overall, our study reveals the effectiveness of antimicrobial plant extracts as therapeutic solutions for antibiotic resistance in Egypt and worldwide with some modifications to decrease their cytotoxicity.</p>","PeriodicalId":9090,"journal":{"name":"Brazilian Journal of Microbiology","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-01-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142944232","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Expression of a periplasmic β-glucosidase from Yarrowia lipolytica allows efficient cellobiose-xylose co-fermentation by industrial xylose-fermenting Saccharomyces cerevisiae strains.","authors":"Angela A Santos, Leonardo G Kretzer, Erika D R Dourado, Carlos A Rosa, Boris U Stambuk, Sérgio L Alves","doi":"10.1007/s42770-024-01609-2","DOIUrl":"https://doi.org/10.1007/s42770-024-01609-2","url":null,"abstract":"<p><p>This study aimed to compare the effects of cellobiose hydrolysis, whether occurring inside or outside the cell, on the ability of Saccharomyces cerevisiae strains to ferment this sugar and then apply the most effective strategy to industrial S. cerevisiae strains. Firstly, two recombinant laboratory S. cerevisiae strains were engineered: CEN.PK-X-Bgl1YL, expressing the periplasmic β-glucosidase BGL1 from Yarrowia lipolytica; and CEN.PK-X-B7-T2, co-expressing the intracellular β-glucosidase SpBGL7 from Spathaspora passalidarum and the cellobiose transporter MgCBT2 from Meyerozyma guilliermondii. Both engineered strains were able to grown in media with cellobiose and to ferment this disaccharide. However, CEN.PK-X-Bgl1YL, which hydrolyzes cellobiose extracellularly, exhibited faster growth and superior batch fermentation performance. Furthermore, enzymatic and transport activities revealed that sugar uptake was possibly the limiting factor in cellobiose fermentation by CEN.PK-X-B7-T2. Since extracellular hydrolysis with the periplasmic β-glucosidase was more efficient for cellobiose fermentation, we integrated the BGL1 gene into two industrial xylose-fermenting S. cerevisiae strains. The resulting strains (MP-C5H1-Bgl1YL and MP-P5-Bgl1YL) efficiently co-consumed ∼ 22 g L^- 1 of cellobiose and ∼ 22 g L^- 1 of xylose in 24 h, achieving high ethanol production levels (∼ 17 g L^- 1 titer, ∼ 0.50 g L^- 1 h^- 1 volumetric productivity, and 0.40 g g^- 1 ethanol yield). Our findings suggest that the expression of periplasmic β-glucosidases in S. cerevisiae could be an effective strategy to overcome the disaccharide transport problem, thus enabling efficient cellobiose fermentation or even cellobiose-xylose co-fermentation.</p>","PeriodicalId":9090,"journal":{"name":"Brazilian Journal of Microbiology","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2024-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142908859","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The efficacy of egg albumin nanoparticles adjuvanted Clostridium perfringens type D toxoid vaccine in rabbits.","authors":"Mehwish Saeed, Saad Zafar, Zarreen Sajjad, Rizwan Aslam, Sultan Ali, Muhammad Shahid Mahmood, Mueed Aayan, Maria Sophy, Saqib Umer, Sajjad Ur Rahman, Muhammad Naveed Anwar","doi":"10.1007/s42770-024-01589-3","DOIUrl":"https://doi.org/10.1007/s42770-024-01589-3","url":null,"abstract":"<p><p>Epsilon toxin (ETX) is an exotoxin produced by Clostridium perfringens type D that induces enterotoxaemia or necrotic intestinal infection in small ruminants and bovine. Immunization is an essential element in preventing the spread of infectious diseases. In recent literature, nanocarriers have exhibited the capacity to deliver protection, stability, and regulated distribution properties to protein-based antigens. Furthermore, egg albumin is a highly adaptable protein nanocarrier in vaccine delivery systems due to its biocompatible, biodegradable, non-toxic, and non-immune-modulating properties. In this study, we assessed the efficacy, safety, immunogenicity, and dose-effect relationships of the nanoparticle-advanced toxoid vaccine (G1) in contrast to the commercially available vaccine (ETV) (G2). Two different vaccines (1 ml) were inoculated in experimental animals (rabbits) on days 1, 7, 14, 21, and 28. The geometric mean titers (GMT) of Groups 2 and 3 were recorded on the respective day of inoculation. The findings reveal that the GMT of group 2 was significantly higher than group 3. The use of nanoparticles to detain toxins demonstrated enhanced immune protection against the harmful effects caused by the toxins. This work is anticipated to explore new opportunities in developing improved vaccinations using nanoparticles to combat the pathogenicity/ virulence factors that present potential risks to livestock.</p>","PeriodicalId":9090,"journal":{"name":"Brazilian Journal of Microbiology","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2024-12-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142892060","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Efficiency of mycorrhizal fungi for seed germination and protocorms development of commercial Cattleya species (Orchidaceae).","authors":"Pedro T S Nogueira, Emiliane F S Freitas, Jessica A R Silva, Maria C M Kasuya, Olinto L Pereira","doi":"10.1007/s42770-024-01597-3","DOIUrl":"https://doi.org/10.1007/s42770-024-01597-3","url":null,"abstract":"<p><p>Orchidaceae is one of the largest plant families and stands out for its wide variety of flowers with ornamental and environmental importance. Cattleya is one of the main commercial genera, presenting a great diversity of species and hybrids that attract the attention of collectors, orchid enthusiasts, and consumers. In their natural environment, orchids associate with mycorrhizal fungi, which are responsible for providing carbon and other nutrients during seed germination. This study investigated the potential of mycorrhizal fungi isolated from the genus Cattleya for in vitro symbiotic germination of seeds from three contrasting Cattleya species, comparing them with non-symbiotic germination in a commercially used culture medium for orchid propagation. The isolated fungi were molecularly identified through phylogenetic analyses of DNA sequences using the ITS (Internal Transcribed Spacer) region. Three isolates obtained were identified as Tulasnella amonilioides, and through microscopic evaluations, the formation of monilioid cells was observed, a morphological characteristic previously unknown for this species. The T. amonilioides isolates were efficient in promoting seed germination of Cattleya bicolor, Cattleya walkeriana and Cattleya jongheana and accelerated the germination process when compared with the non-symbiotic commercial medium, showing to be promised for commercial seed production of these orchids species.</p>","PeriodicalId":9090,"journal":{"name":"Brazilian Journal of Microbiology","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2024-12-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142892053","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Role of horizontal gene transfer and cooperation in rhizosphere microbiome assembly.","authors":"Simone Raposo Cotta, Armando Cavalcante Franco Dias, Rodrigo Mendes, Fernando Dini Andreote","doi":"10.1007/s42770-024-01583-9","DOIUrl":"https://doi.org/10.1007/s42770-024-01583-9","url":null,"abstract":"<p><p>Microbes employ a variety of mechanisms, encompassing chemical signaling (e.g., quorum-sensing molecules) and genetic processes like horizontal gene transfer (HGT), to engage in interactions. HGT, in particular, holds a pivotal role as it facilitates the generation of metabolic diversity, thus directly or indirectly influencing microorganisms' interactions and functioning within their habitat. In this study, we investigate the correlations between enhanced metabolic diversity through HGT and cooperative behavior in the rhizosphere. Despite the potential drawbacks of cooperative behavior, which renders it susceptible to exploitation by cheaters based on evolutionary theory, HGT emerges as a mitigating factor. It serves as a valuable and adaptive tool for survival in competitive environments, notably the rhizosphere. By initiating a comprehensive discussion on these processes combined, we anticipate achieving a profound understanding of the rhizosphere microbiome, ultimately enhancing soil microbiology management and the exploitation of this ecological niche.</p>","PeriodicalId":9090,"journal":{"name":"Brazilian Journal of Microbiology","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2024-12-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142892056","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Kraft lignin biobleaching by a dye-decolorizing peroxidase from the Antarctic Pseudomonas sp. AU10 strain.","authors":"Célica Cagide, Diego Vallés, Susana Castro-Sowinski","doi":"10.1007/s42770-024-01595-5","DOIUrl":"https://doi.org/10.1007/s42770-024-01595-5","url":null,"abstract":"<p><p>Pseudomonas sp. AU10 is an Antarctic psychrotolerant bacterium that produces a dye-decolorizing peroxidase (DyP-AU10). The recombinant enzyme (rDyP-AU10) is a heme-peroxidase that decolors dyes and modifies kraft lignin. In this work, we report the best activity parameters for lignin modification (at 45 °C and pH 4) and show that the enzyme increases the number of aldehydes, ketones, and phenolic compounds. The analyses of the HPLC profile of samples also support that rDyP-AU10 induces the chemical change of kraft lignin. The enzyme also acts as a biobleaching agent on cellulose pulps, as shown by the reduction in kappa number. We also included experiments with a commercial laccase from Trametes versicolor and performed experiments using single enzymes and, in combination. The results show that rDyP-AU10 and the commercial laccase do not have a synergic activity as a modifying system, on cellulose pulp as substrates. However, results suggest that rDyP-AU10 holds potential as a member of the portfolio of lignin-modifying enzymes.</p>","PeriodicalId":9090,"journal":{"name":"Brazilian Journal of Microbiology","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2024-12-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142881107","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}