Brazilian Journal of Microbiology最新文献

{"title":"Revisiting the emergence of the Chikungunya virus in Alagoas, Northeast of Brazil.","authors":"Mykaella Andrade de Araújo, Ana Carla Eugênio Lima, Jean Phellipe Marques do Nascimento, Ana Paula Andreolla, Thiago Pina Goes de Araújo, Michel Alves do Nascimento, Ana Rachel Vasconcelos de Lima, Magliones Carneiro de Lima, Hazerral de Oliveira Santos, Anderson Brandão Leite, Juliano Bordignon, Claudia Nunes Duarte Dos Santos, Abelardo Silva-Júnior, Alessandra Abel Borges","doi":"10.1007/s42770-025-01705-x","DOIUrl":"10.1007/s42770-025-01705-x","url":null,"abstract":"<p><p>Chikungunya virus (CHIKV), an Alphavirus, emerged in the Americas in 2013 and was first documented in Brazil in September 2014, in the states of Pará and Bahia. Although Alagoas state officially reported its first case in late 2015, this study investigated potential earlier unreported cases by analyzing samples from a 2013-2014 Orthoflavivirus serological survey. We screened sera from patients with acute febrile illness, initially suspected of dengue but negative in molecular tests for the genus Orthoflavivirus, using ELISA (IgM/IgG), viral isolation, PCR, and next-generation sequencing. Two samples collected in June and August 2014 tested positive for anti-CHIKV IgM, and four additional samples collected between June and September 2014 tested positive for anti-CHIKV IgG antibodies. From one sample IgM-positive (630H) collected in August 2014, we isolated and sequenced a nearly complete genome (95.53% coverage, 2714× depth) classified as the East-Central-South-African (ECSA) genotype. Phylogenetic analyses revealed that the Alagoas-2014 genome formed a distinct, well-supported clade separate from the Bahia 2014 lineage. Temporal inference dated this lineage's origin to October 2013 (90% CI: April 2013-March 2014), suggesting a new introduction of ECSA into Alagoas. This genomic evidence, along with serological data, confirms the undetected early circulation of CHIKV in Alagoas and suggests a possible introduction of ECSA in Brazil in 2014, distinct from the well-documented introduction in Bahia.</p>","PeriodicalId":9090,"journal":{"name":"Brazilian Journal of Microbiology","volume":" ","pages":"1901-1911"},"PeriodicalIF":1.9,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12350965/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144156843","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prevalence and antibiotic resistance of extended spectrum β-lactamase producing Escherichia coli in sheep and slaughterhouse environment in Kayseri, Türkiye: A special focus on β-lactamase genes.","authors":"Kursat Koskeroglu, Yeliz Ucar","doi":"10.1007/s42770-025-01708-8","DOIUrl":"10.1007/s42770-025-01708-8","url":null,"abstract":"<p><p>Antimicrobial resistance is a worldwide threat that causes serious health problems in both humans and animals. There is limited information regarding the prevalence of extended-spectrum β-lactamase (ESBL) producing Escherichia coli in small ruminants. The purpose of this study was to determine the prevalence, and antibiotic resistance profiles of ESBL E. coli in sheep and the slaughterhouse environment in Kayseri, Türkiye. E. coli isolates were further screened for the presence of ESBL and carbapenemase-encoding genes (bla_CTX-M, bla_SHV, bla_TEM, bla_KPC, bla_NDM, and bla_OXA-48). A year-long study was conducted to include all seasons. Samples from sheep included feces, carcass swabs, paddock feces, and cecum content (n = 108) while samples from the slaughterhouse included surface and wastewater samples (n = 76). McConkey agar containing 4 µg/ml of cefotaxime was used to isolate ESBL E. coli. A double disk-diffusion test was performed to confirm β-lactamase production. Using the conventional method, 40.7% (44/108) isolates from sheep and 23.7% (18/76) isolates from environmental samples were tested to be positive. The prevalence of ESBL E. coli in sheep samples was significantly higher in summer (70.4%) than in spring (40.7%), autumn (33.3%), and winter (18.5%) (P₁ < 0.001). In regard to β-lactamase genes; bla_TEM 62 (100%), bla_CTX-M 56 (90.3%), and bla_SHV 2 (3.2%) were found positive. Of the 15 (24.2%) isolates tested to be positive for the bla_OXA-48 gene, none had the ability to produce carbapenamase. Most isolates harbored multiple genes with bla_TEM + bla_CTX-M was observed in 40 (64.5%), bla_TEM + bla_CTX-M + bla_OXA-48 in 13 (20.9%), and bla_TEM + bla_CTX-M + bla_OXA-48 + bla_SHV in 2 isolates (3.2%). Based on antibiotic resistance testing, all ESBL-producing isolates were found to be resistant to ampicilin and ceftazidime. Resistance to cloramphenicol 36 (58.1%) was evidently followed by gentamicin 27 (43.5%), nalidixic acid 27 (43.5%), ciprofloxacin 25 (40.3%), amoxicillin-clavulanic acid 7 (11.3%), azithromycin 6 (9.7%) and imipenem 4 (6.5%). The results indicated 43 (69.4%) isolates to be multidrug-resistant.</p>","PeriodicalId":9090,"journal":{"name":"Brazilian Journal of Microbiology","volume":" ","pages":"1709-1720"},"PeriodicalIF":1.9,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12350987/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144274211","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sarcocystid pathogens in captive monkeys from southern Brazil.","authors":"Gisele Vaz Aguirre Samoel, Fagner D'ambroso Fernandes, Helton Fernandes Dos Santos, Juliana Felipetto Cargnelutti, Fernanda Silveira Flores Vogel","doi":"10.1007/s42770-025-01738-2","DOIUrl":"10.1007/s42770-025-01738-2","url":null,"abstract":"<p><p>Non-human primates (NHP) can serve as intermediate or accidental hosts for sarcocystid pathogens, which vary in pathogenicity and relevance to wildlife and public health. This study aimed to detect DNA from the members of the Sarcocystidae family in tissue samples from 22 captive monkeys that died between 2019 and 2023 in a zoo located in southern Brazil. A total of 84 samples were analyzed using nested PCR, restriction fragment length polymorphism (RFLP), and specific primers to identify Sarcocystis spp., Toxoplasma gondii, Neospora sp., and Hammondia sp. According to our results, the DNA of sarcocystid pathogen was detected in 10 animals (45.45%), including T. gondii (18.18%), Sarcocystis sp. (27.27%) and H. hammondi (4.54%). One capuchin monkey developed fatal disseminated toxoplasmosis caused by an atypical T. gondii genotype, characterized by multilocus PCR-RFLP and submitted to ToxoDB. Additionally, DNA from H. hammondi was detected in a lung sample of a white-tufted marmoset (Callithrix jacchus), suggesting that this species may act as an intermediate host. These findings contribute to the understanding of sarcocystid infections in NHPs under human care and underscore the importance of surveillance in zoological institutions, especially regarding T. gondii diversity in South America.</p>","PeriodicalId":9090,"journal":{"name":"Brazilian Journal of Microbiology","volume":" ","pages":"1611-1617"},"PeriodicalIF":1.9,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12350851/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144658320","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biosynthesis in Streptomyces sp. NRRL S-1813 and regulation between oxazolomycin A and A2.","authors":"Qingyun Zhang, Daotong Lv, Huiyu Gong, Jiayi Ren, Yunbin Lyu, Shaochen Wang, Zhiyang Feng","doi":"10.1007/s42770-025-01735-5","DOIUrl":"10.1007/s42770-025-01735-5","url":null,"abstract":"<p><p>A novel actinomycete strain, Streptomyces sp. NRRL S-1813 was employed to study its secondary metabolites under different mediums to activate its cryptic gene clusters and produce antimicrobial secondary metabolites. During fermentation optimization, and purification, oxazolomycin A and oxazolomycin A2 were isolated from one strain simultaneously. Their structure was elucidated using a series of characterization techniques, including full wavelength scanning, mass spectrometry (MS), and nuclear magnetic resonance (NMR) spectroscopy. Oxazolomycin A2 was found not to be a typical enzymatic product of fermentation process. Instead, a spontaneous, non-enzymatic ring cleavage reaction was identified as mechanism for conversion of oxazolomycin A to oxazolomycin A2. Basing on these results, if the target product is oxazolomycin A2, the best fermentation condition of Streptomyces sp. NRRL S-1813 should be the Medium B under the alkalescence condition. For the biosynthesis of oxazolomycin A, the medium pH and reaction time were both important. A slightly acidic environment suppresses the side reactions such as hydrolysis of product, while reasonable reaction time minimizes accumulation of byproducts.</p>","PeriodicalId":9090,"journal":{"name":"Brazilian Journal of Microbiology","volume":" ","pages":"1519-1528"},"PeriodicalIF":1.9,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12350879/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144607311","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corynebacterium pseudodiphtheriticum as etiologic agent of human infections worldwide: a review of case reports within the last 40 years (1983-2023).","authors":"Marcos Andrade-Silva, Louisy Sanches Dos Santos, Ana Luíza Mattos-Guaraldi","doi":"10.1007/s42770-025-01739-1","DOIUrl":"10.1007/s42770-025-01739-1","url":null,"abstract":"<p><p>Corynebacterium pseudodiphtheriticum has been increasingly reported as an etiologic agent of both community-acquired and nosocomial infections, despite its common presence as a harmless member of the human upper respiratory tract and skin microbiota. However, infections caused by C. pseudodiphtheriticum and other corynebacterial species are still frequently misclassified as contamination and overlooked during clinical and laboratory diagnoses in several countries. In this study, an extensive review of human infections and nosocomial outbreaks caused by C. pseudodiphtheriticum was conducted through the analysis of reported cases from various countries, over a 40-year period (1983-2023). This review highlights the dichotomous nature of C. pseudodiphtheriticum, emphasizing both its potential biotechnological applications and its role as a human pathobiont affecting immunocompromised and immunocompetent individuals worldwide. Therefore, this study supports the inclusion of C. pseudodiphtheriticum in the list of clinically relevant pathogens and underscores the need for research into the virulence mechanisms involved in its pathogenesis.</p>","PeriodicalId":9090,"journal":{"name":"Brazilian Journal of Microbiology","volume":" ","pages":"1929-1947"},"PeriodicalIF":1.9,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12350908/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144641801","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Veterinary application of LAMP: a simple and visual detection tool for feline sporotrichosis.","authors":"Lívia do Carmo Silva, Isabela Cristina de Oliveira Dos Santos, Juliana Santana de Curcio, Allana de Paula Castilho, João Vitor Gregolan Barbosa, Luiz Henrique Alves Costa, Álvaro Ferreira Júnior, Elisângela de Paula Silveira-Lacerda","doi":"10.1007/s42770-025-01694-x","DOIUrl":"10.1007/s42770-025-01694-x","url":null,"abstract":"<p><p>Early diagnosis of sporotrichosis, along with the surveillance of fungal circulation in both human and domestic animal populations, is critical for global health. Although the gold standard method is microbiological culture followed by microscopic analysis of the fungus, this methodology requires a long period and skilled professionals for pathogen identification. We developed a molecular assay and conducted a preliminary evaluation using clinical samples using Loop-Mediated Isothermal Amplification (LAMP) technology that detects Sporothrix schenckii and Sporothrix brasiliensis fungi, with results in 30 min. Results are visually interpreted and do not require DNA extraction, making it a promising tool for point-of-care diagnosis in veterinary settings.</p>","PeriodicalId":9090,"journal":{"name":"Brazilian Journal of Microbiology","volume":" ","pages":"1483-1493"},"PeriodicalIF":1.9,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12350994/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144156844","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antimicrobial potential and extracellular metabolite profiling of bichitrapur mangrove sediment derived Micromonospora sp. BSS-D-04 and Streptomyces sp. BSS-D-05 using LC-MS/MS and GC-MS analysis.","authors":"Dipransu Pradhan, Mihir Tanay Das","doi":"10.1007/s42770-025-01710-0","DOIUrl":"10.1007/s42770-025-01710-0","url":null,"abstract":"<p><p>Terrestrial actinomycetes have historically dominated the production of antimicrobial compounds. However, recent investigations into aquatic strains, particularly those from marine habitats, have revealed a vast reservoir of bioactive compounds with potential therapeutic applications. This study focuses on actinomycetes isolated from Bichitrapur Mangrove sediments which creates an extreme habitat for the microorganisms. Six distinct colonies were isolated, and their potential for antimicrobial compound production was explored. The investigation integrates molecular and chemical analyses, employing genetic sequencing for taxonomic identification and gas chromatography-mass spectrometry (GC-MS) and liquid chromatography-mass spectrometry (LC-MS/MS) for compound characterization. The 16S rRNA gene sequences of BSS-D-04 and BSS-D-05 were submitted to the NCBI database, confirming their identities as Micromonospora sp. and Streptomyces sp., respectively. Phylogenetic analysis provided insight into the evolutionary relationships of these isolates. The organic solvent extracts from the selected strains were subjected to GC-MS and LC-MS/MS analyses, revealing many novel bioactive compounds. The identified compounds, documented for their broad-spectrum biological activities, encompassed antimicrobial, antifungal, insecticidal, antioxidative, anti-inflammatory, anticancer, antiviral, and antiparasitic properties. Especially, the study highlighted the higher pharmaceutical potential of Micromonospora sp. compared to Streptomyces sp. which is responsible for the 70% of the total discovered antibiotics. Hence, this comprehensive investigation sets the stage for further exploration and purification of metabolites from these highly potent actinobacterial strains which will help in new drug development and potential applications in the pharmaceutical industry.</p>","PeriodicalId":9090,"journal":{"name":"Brazilian Journal of Microbiology","volume":" ","pages":"1843-1859"},"PeriodicalIF":1.9,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12351003/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144474014","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification of additional probiotic attributes in yeasts isolated from tarhana fermentation.","authors":"Belgin Erdem, Halil İbrahim Kaya, Şener Tulumoğlu, Ebru Coteli, Ömer Şimşek","doi":"10.1007/s42770-025-01728-4","DOIUrl":"10.1007/s42770-025-01728-4","url":null,"abstract":"<p><p>Fermented foods constitute a valuable source of probiotics for both bacteria and yeasts. To date, however, there has been a limited amount of research conducted on Saccharomyces cerevisiae, Kazakhstania servazzi, Kluyveromyces marxianus and Torulaspora delbrueckii isolated from tarhana. The objective of the research is to identify additional probiotic characteristics other than the leavening activity exhibited by yeasts that were previously isolated from tarhana fermentation. In this study, yeasts were subjected to subsequent acid and bile salt tolerance, bile salt hydrolysis, antagonistic activity, aggregation activity (auto-aggregation and co-aggregation), cholesterol assimilation, folate production, biofilm production, and hemolysis activity. S. cerevisiae PCF122, S. cerevisiae PCF107, and S. cerevisiae PCF134 strains grew at pH 2 and 2,5 but remained at pH 3. Except for S. cerevisiae PCF115 and T. delbrueckii PCF150, all yeasts were found to be 0,5% and 1,0% oxalate tolerant. All yeasts hydrolyze oxalate (bile salt), but only S. cerevisiae PCF115 and T. delbrueckii PCF150 produced EPS. Yeasts also exhibited significant amounts of autoaggregation (46-87%). After 24 and 48 h incubation, all strains assimilated cholesterol at rates ranging from 10,4% to 87,5% and 10,6-91%, respectively. The highest folate production was determined at S. cerevisiae PCF108 (56 µg/mL) and the lowest was at S. cerevisiae PCF110 and K. marxianus PFC120 (18 µg/mL). In conclusion, yeasts that existed in tarhana fermentation showed cholesterol assimilation, folate production, and aggregation activity which are additional probiotic attributes that would have consumer health promotion, beside these yeast leaven the tarhana dough.</p>","PeriodicalId":9090,"journal":{"name":"Brazilian Journal of Microbiology","volume":" ","pages":"1765-1773"},"PeriodicalIF":1.9,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12350889/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144590406","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fungal diversity present in ornithogenic soils of extreme equatorial Atlantic São Pedro and São Paulo archipelago using DNA metabarcoding.","authors":"Vívian Nicolau Gonçalves, Fábio Oliveira Soares, Guilherme Resende Corrêa, Eduardo Osório Senra, Fabyano A C Lopes, Micheline C Silva, Peter Convey, Paulo E A S Câmara, Alysson Wagner Fernandes Duarte, Luiz Henrique Rosa","doi":"10.1007/s42770-025-01698-7","DOIUrl":"10.1007/s42770-025-01698-7","url":null,"abstract":"<p><p>We evaluated the fungal diversity present in ornithogenically-influenced soils of the polyextreme Brazilian Archipelago of São Pedro and São Paulo, using a DNA metabarcoding approach. We detected 1,596,919 fungal DNA reads grouped into 232 amplicon sequence variants (ASVs). The phylum Ascomycota was the dominant phylum, followed by Basidiomycota, Blastocladiomycota, Mortierellomycota, Mucoromycota, Aphelidiomycota, Rozellomycota, Chytridiomycota and Zoopagomycota, in rank abundance order. Cladosporium sp., Hortaea werneckii, Penicillium sp., Aspergillus sp. and Fungal sp. were the dominant assigned taxa. The fungal assemblages displayed high diversity indices, although differing between the sites sampled. Fifty-four fungal ASVs could only be assigned to higher taxonomic levels, primarily those of the cryptic and poorly known phyla Aphelidiomycota, Blastocladiomycota, Chytridiomycota and Rozellomycota. These may represent taxa not currently included in the available databases or new taxa and/or new records for the São Pedro and São Paulo archipelago. Only Cladosporium sp., Hortaea werneckii, Blastobotrys serpentis and Penicillium sp. were detected in all sites. Even though the São Pedro and São Paulo archipelago experiences extreme environmental conditions, the use of metabarcoding revealed a diverse and complex fungal community potentially present in the samples examined. The assigned fungal communities were dominated by genera which commonly display high adaptive plasticity when facing challenging and extreme conditions. The melanized yeast H. werneckii, known for its high resistance to polyextreme conditions, illustrates that this archipelago represents a potential source of extremophilic fungi. The dominant assigned fungi include taxa with different ecological roles, with some also recognized as potentially important animal pathogens.</p>","PeriodicalId":9090,"journal":{"name":"Brazilian Journal of Microbiology","volume":" ","pages":"1619-1629"},"PeriodicalIF":1.9,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12350997/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144172124","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antimicrobial susceptibility and genetic profiles of Escherichia coli isolated from poultry hatcheries in the state of Rio Grande do Sul, Brazil.","authors":"Jônatas Grellmann Breunig, Kelly Cristina Tagliari de Brito, Tamara Flores, Thales Quedi Furian, Karen Apellanis Borges, Hamilton Luiz de Souza Moraes, Carlos Tadeu Pippi Salle, Luciana Kazue Otutumi, Luís Eduardo de Souza Gazal, Gerson Nakazato, Vanessa Lumi Koga, Renata Katsuko Takayama Kobayashi, Camila de Cuffa Matusaiki, Gabriela Rocha Santos, Benito Guimarães de Brito","doi":"10.1007/s42770-025-01688-9","DOIUrl":"10.1007/s42770-025-01688-9","url":null,"abstract":"<p><p>Escherichia coli is widely distributed in human and animals as part of commensal microbiota, but some isolates can act as major pathogens causing severe diseases. Data regarding the characterization of E. coli isolated from hatchery is limited. Extensive genetic similarity among isolates has been previously reported, including similar virulence and antimicrobial resistance genes, which make it difficult to differentiate between pathogenic and commensal bacteria. The aim of this study was to characterize 84 E. coli isolates from four hatcheries in Rio Grande do Sul state (Brazil) for antimicrobial susceptibility, detection of multidrug resistance (MDR) and extended-spectrum β-lactamases (ESBL) isolates, and phylogenetic groups. Most antimicrobial agents presented moderate (20-50%) or high (> 50%) resistance rates, and 58 phenotypic resistance profiles were observed. A total of 51.19% and 20.24% of isolates were classified as MDR and ESBL, respectively. The majority of the isolates (80.95%) were classified in the pathogenic phylogroups (B2 and D), regardless the hatchery establishment. The hatchery did not influence in any of the results. The results demonstrate the high antibiotic resistance demonstrated by E. coli isolated from hatcheries in southern Brazil. The dissemination of pathogenic isolates in all establishments evaluated is a matter of particular concern. In this context, the results found in this study are important and may be the first step on monitoring pathogenicity and antimicrobial resistance fluctuation in Brazilian hatcheries.</p>","PeriodicalId":9090,"journal":{"name":"Brazilian Journal of Microbiology","volume":" ","pages":"2155-2162"},"PeriodicalIF":1.9,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12350989/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144172753","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}