Brazilian Journal of Microbiology最新文献

{"title":"Molecular detection of respiratory viruses: an observational study on respiratory co-infections in children and adults.","authors":"Tereza de Jesus Pinheiro Gomes Bandeira, Ana Leticia Sousa de Oliveira, Luis Fernando Pontes Martins, Rodrigo Moreira Matos, Sheryda Raynna Nobre Guedes Dos Santos, Mateus Cedro Lopes, Raimundo Tadeu Pires Sobreira, Hermano Alexandre Lima Rocha","doi":"10.1007/s42770-024-01581-x","DOIUrl":"https://doi.org/10.1007/s42770-024-01581-x","url":null,"abstract":"<p><p>Respiratory tract infection (RTI) is the most important cause of visiting both public and private medical care services. During the decade preceding Covid-19, in developing countries, there was a gap in the epidemiology of respiratory viral infections, probably due to the difficulty of detecting viral agents in clinical microbiology laboratories routine, at that time. In this context, the aim of this study was to describe the occurrence of virus, demographic factors associated and the likelihood of coinfections detection by multiplex PCR methods. The target also was to determine the involvement of respiratory viruses other than SARS-CoV-2 as agents of upper respiratory tract infections (URTIs) using rapid respiratory panels by multiplex real time PCR with flow chip methodology. Samples of SARSs patients, 530 in total, were tested between 2022 and 2023, obtained from nasopharyngeal swabs, nasopharyngeal exudates or nasopharyngeal aspirates. Of 530 tests, 30% was human rhinovirus, the most prevalent, followed by respiratory syncytial virus B (21.5%) and human adenovirus (17.4%). Among the total of positive samples, 83.8% belonged to patients aged between 0 and 10 years, 62.6% of whom were aged between 1 and 10 years. Multiple virus detection was found in 50.4% of the samples tested, 93.1% of which were from patients under the age of 18. Viral co-infection was evident in the finding and was higher (88.7%) in patients under the age of 18 yeas.</p>","PeriodicalId":9090,"journal":{"name":"Brazilian Journal of Microbiology","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2024-12-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142845981","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Viability of microencapsulated species of Trichoderma as a strategy to optimize use in biological control.","authors":"Thalesram Izidoro Pinotti, Tiago de Oliveira Sousa, Wéverson Lima Fonseca, Thiago Pajeú Nascimento, Helane França Silva, Romero Marcos Pedrosa Brandão Costa, Alice Maria Gonçalves Santos","doi":"10.1007/s42770-024-01594-6","DOIUrl":"https://doi.org/10.1007/s42770-024-01594-6","url":null,"abstract":"<p><p>The increasing use of chemical fungicides without effective control of phytopathogens has led to the development of resistance in microorganisms. As a promising alternative, products formulated with Trichoderma have emerged for their sustainable and effective potential in integrated disease management. However, the predominant formulations do not offer the necessary protection against abiotic factors. In this study, we investigated Trichoderma species encapsulated in sodium alginate through storage viability experiments and their antagonistic potential. The viability and storage conditions of the capsules were evaluated by plating at 5, 15, 30, 45, 60, and 150 days after production, kept in dry or refrigerated environments. The antagonistic potential was determined by the culture pairing method using the phytopathogen Fusarium sp. The results demonstrated that it was possible to maintain the viability of the conidia, with no differences between storage environments. Additionally, the capsule provided UV protection to the conidia encapsulated species possess antagonistic potential, inhibiting 52.54% of Fusarium sp. growth. Consequently, encapsulation is an alternative formulation method that ensures the viability of Trichoderma conidia and optimizes its use in biological control.</p>","PeriodicalId":9090,"journal":{"name":"Brazilian Journal of Microbiology","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2024-12-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142845983","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular genetic characterization of porcine reproductive and respiratory syndrome virus outbreak in Assam, India and neighbouring regions.","authors":"Seema Rani Pegu, Joyshikh Sonowal, Pranab Jyoti Das, Swaraj Rajkhowa, Manjisa Choudhury, Rajib Deb, Gyanendra S Sanger, Ajay K Yadav, Nabajyoti Deka, Sunil Kumar, Vivek K Gupta","doi":"10.1007/s42770-024-01592-8","DOIUrl":"https://doi.org/10.1007/s42770-024-01592-8","url":null,"abstract":"<p><p>Porcine reproductive and respiratory syndrome (PRRS) is a significant swine disease with no effective vaccine due to high viral mutation rates. This study investigates a natural PRRS outbreak through molecular, pathological, and serological analyses. Nineteen affected pigs were clinically examined, and 10 underwent post-mortem examination. PRRS virus (PRRSV) presence was confirmed in all tissue samples by RT-PCR targeting open reading frame (ORF) 5 and ORF7 genes. Clinical signs, especially in boars and sows, included fever, appetite loss, movement reluctance, erythematous skin patches, vomiting, and abortions in sows. Post-mortem findings highlighted lung consolidation, severe lymph node enlargement, interstitial pneumonia with mononuclear cells, macrophage accumulation and necrotic cells in alveolar spaces. Multifocal myocarditis, lymphoid follicular degeneration, and follicular necrosis were observed in the tonsil, spleen, and lymph nodes. PRRSV-specific antibodies were detected in 32.75% of serum samples, confirming the outbreak. Phylogenetic analysis of the PRRSV-ORF5 and ORF7 genes revealed a close genetic relationship between the outbreak samples from Assam and recent outbreaks in Idukki, Kerala, India (2018), and neighbouring country China, indicating the circulation of Genotype 2 virus in Assam. However, the sequences showed some differences from the isolates of Mizoram, India. In conclusion, this study provides molecular and pathological evidence of a PRRSV outbreak, confirms the presence of PRRSV-specific antibodies and viral RNA, and shed light on the virus's genetic characteristics in India.</p>","PeriodicalId":9090,"journal":{"name":"Brazilian Journal of Microbiology","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2024-12-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142845982","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chemical profile, antioxidant, antifungal, and cytotoxic activities of propolis from the stingless bee Tetragona clavipes.","authors":"Ariane Pinheiro Cruz Bergamini, Brendo Victor Siqueira de Almeida Bergamini, Iana Soares Pessoa, Thiago Antônio de Sousa Cutrim, Tamires Cruz Dos Santos, Matheus Campos Dos Santos, Victor da Rocha Fonseca, Wanderson Romão, Rodrigo Scherer, Denise Coutinho Endringer, Marcio Fronza","doi":"10.1007/s42770-024-01591-9","DOIUrl":"https://doi.org/10.1007/s42770-024-01591-9","url":null,"abstract":"<p><p>Propolis is derived from plant resin and bees' salivary secretions, are commonly used in folk medicine. Studies on propolis from stingless bees have increased in recent years, highlighting their relevance due to the variety of species and the bioactive components. However, scientific records confirming these activities in species occurring in Brazil are still scarce. In that context, this study aims to determine the chemical profile and the antioxidant, antifungal, and cytotoxic activities of the stingless bees Tetragona clavipes propolis. The hydroalcoholic extract was prepared, and its chemical profile was determined by Fourier transform ion cyclotron resonance mass spectrometry combined with a direct infusion electrospray ionization. Total polyphenols and flavonoid content were determined by colorimetric methods. We determined the antioxidant activity by the ability to scavenge the free radicals 2,2-diphenyl-1-picrylhydrazyl and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid). The antifungal activity was evaluated by determining the minimum inhibitory concentration and minimum fungicidal concentration against Aspergillus fumigatus, Trichophyton rubrum, Candida albicans, and Candida parapisilosis. The results revealed high contents of total phenolics and promising antioxidant and antifungal activities. T. clavipes propolis from the rainy season showed better antioxidant and antifungal activities and higher content of flavonoids, with less variability of chemical species. The extracts exhibited fungistatic activity for C. albicans, C. parapisilosis, and A. fumigatus and fungicide for T. rubrum. Additionally, a sample collected during the rainy season demonstrated synergism with fluconazole and amphotericin B for T. rubrum and additivity for C. albicans and C. parapisilosis. In summary, the results of T. clavipes propolis revealed elevated levels of total phenolics, indicating its potential as a rich source of bioactive compounds, significant antioxidant activity, and promising antifungal activity against common pathogenic fungi. These findings contribute to our understanding of the therapeutic potential of T. clavipes propolis and provide a basis for further research and development of natural products for various applications in medicine and healthcare.</p>","PeriodicalId":9090,"journal":{"name":"Brazilian Journal of Microbiology","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2024-12-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142821747","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sulfur fertilization and inoculation of soybean with Azospirillum brasilense and Bradyrhizobium spp. can improve grain yield and quality.","authors":"Evandro Ademir Deak, Thomas Newton Martin, Jessica Deolinda Leivas Stecca, Gerusa Mussuquini Conceição, Matheus Martins Ferreira, Vítor Sauzem Rumpel, Acir Felipe Grolli Carvalho, Francisco Javier López Baena","doi":"10.1007/s42770-024-01585-7","DOIUrl":"https://doi.org/10.1007/s42770-024-01585-7","url":null,"abstract":"<p><p>The success of biological nitrogen fixation (BNF) in soybean depends on two crucial factors, viz., seed inoculation and soil nutritional balance. The macronutrient sulfur (S) is vital to the formation of ferredoxin, a common source of electrons that controls the proper functioning of the subunits of the enzyme nitrogenase, responsible for the conversion of atmospheric nitrogen (N₂) to ammonia (NH₃⁺). However, as the S dynamics is a complex process in soil, it may cause to the plants to be sulfur limited. This study aims at assessing the relationship between S fertilization through the use of elemental-S, and bacterial inoculation (Bradyrhizobium spp.) and co-inoculation (Bradyrhizobium spp. and Azospirillum brasilense) on nodulation, production and quality of soybean. The study was performed on the 2017/2018 and 2018/2019 crop seasons, involving four experiments where two were carried out in Santa Maria and two in Augusto Pestana, Rio Grande do Sul, Brazil. Adopting the randomized experimental block design, the treatments included a 3 × 4 factorial design, with three inoculations (Non-inoculated control, Inoculation and Co-inoculation) together with four doses of S (0, 20, 40 and 60 kg ha^- 1). Evaluations were done of the plant nodulation, accumulation of shoot dry matter, yield constituents, and quality of the soybean grain. S fertilization and co-inoculation promote an increase in plant nodulation, proving to be an important strategy to support nitrogen supply to soybean crops. The application of elemental sulfur in doses between 20 and 40 kg ha^- 1 promotes nodulation, the accumulation of dry mass of plants, the productivity and quality of soybeans, in addition to benefiting nodulation when combined with co-inoculation of Bradyrhizobium ssp. and Azospirillum brasilense.</p>","PeriodicalId":9090,"journal":{"name":"Brazilian Journal of Microbiology","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2024-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142811844","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Anti-Pythium insidiosum activity of three novel triazole compounds: synthesis, pharmacokinetic and toxicological parameters.","authors":"Carolina Martins Fernandes, Alessandro de Souza Prestes, Lara Baccarin Ianiski, Aline Fontanella Maciel, Bruna Godoy Noro, Fernanda D'Avila da Silva, Bruno Stefanello Vizzotto, Sônia de Avila Botton, Ricardo Frederico Schumacher, Daniela Isabel Brayer Pereira, Nilda Vargas Barbosa","doi":"10.1007/s42770-024-01572-y","DOIUrl":"https://doi.org/10.1007/s42770-024-01572-y","url":null,"abstract":"<p><p>Pythiosis, caused by Pythium insidiosum, is an infectious and non-transmissible disease affecting horses, dogs, and humans, with no effective drug treatment available. Triazoles are compounds of interest for their potential pharmacological properties against fungi and bacteria. In this study, we synthesized three new triazole compounds (C1, C2, and C3) to assess their in vitro activities against P. insidiosum and their safety on human leukocytes. Susceptibility testing was performed against P. insidiosum isolates (n = 15) to determine the minimum inhibitory concentration (MIC) and minimum oomicidal concentration (MOC). The leukocyte toxicity of triazoles was evaluated by measuring cell viability, morphological aspects, and oxidative stress endpoints. In silico prediction of the compounds absorption, distribution, metabolism, excretion and toxicity (ADMET) was determined using the pkCSM platform. Both triazoles C1 and C2 exhibited anti-Pythium insidiosum activity at concentrations from 2 to 64 µg/mL to MIC and MOC, while C3 MIC was 4-64 µg/mL and MOC 8-64 µg/mL. The three compounds did not induce viability loss and/or morphologic changes to human leukocytes, and showed absence of a pro-oxidant profile. ADMET properties prediction of the compounds was similar to the reference drug fluconazole. This study introduces novel triazole compounds exhibiting anti-P. insidiosum activity at concentrations non-toxic to human leukocytes.</p>","PeriodicalId":9090,"journal":{"name":"Brazilian Journal of Microbiology","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2024-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142811720","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Detection of Brno loanvirus (Loanvirus brunaense) in common noctule bats (Nyctalus noctula) in Southern Russia.","authors":"Olesia V Ohlopkova, Kristina A Stolbunova, Ilia V Popov, Igor V Popov, Emmanuel Kabwe, Yuriy N Davidyuk, Marina A Stepanyuk, Alexey D Moshkin, Yulia V Kononova, Ekaterina A Lukbanova, Alexey M Ermakov, Michael L Chikindas, Ivan A Sobolev, Svetlana F Khaiboullina, Aleksandr M Shestopalov","doi":"10.1007/s42770-024-01587-5","DOIUrl":"https://doi.org/10.1007/s42770-024-01587-5","url":null,"abstract":"<p><p>Hantaviruses that infect humans are rodent-derived viruses with zoonotic potential. Several studies show that before emerging in rodents hantaviruses could emerge in bats, which makes it important to study bat-derived hantaviruses. In this study, we performed PCR screening of hantaviruses in samples from common noctules (Nyctalus noctula [182 fecal and 81 blood serum samples]), parti-coloured bats (Vespertilio murinus [41 fecal samples]), Kuhl's pipistrelles (Pipistrellus kuhlii [15 fecal samples]), and serotine bats (Eptesicus serotinus [8 fecal samples]) from Rostov Bat Rehabilitation Center (Rostov-on-Don, Russia) and phylogenetic analysis of detected viruses. As a result, hantaviruses were detected in samples from N. noctula bats with an overall prevalence of 4.94% (4/81, 95% CI 0.22-9.66%) in blood serum samples and 1.1% (2/182, 95% CI 0-2.61%) in fecal samples. Phylogenetic analysis revealed that detected hantaviruses are highly homologic to Brno loanviruses (Loanvirus brunaense) previously discovered in N. noctula bats from Central Europe, which brings some evidence that these are the same bat-derived viruses. This study shows that Loanvirus brunaense could be species-specific to the host and has a wide area of habitat: from Central Europe to Southern Russia. These are the first findings of this virus in Southern Russia and Ciscaucasus/Fore-Caucasus. Further studies with wider screening and genomic assays of Loanvirus brunaense in bats could reveal trends in the molecular evolution of hantaviruses and provide valuable data for the control of potential spillovers.</p>","PeriodicalId":9090,"journal":{"name":"Brazilian Journal of Microbiology","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2024-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142811760","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Analysis of antibiotic susceptibility and genomic characteristics of Helicobacter pylori by whole-genome resequencing in Northern China.","authors":"Yaxuan Wang, Tiantong Jiang, Xiaochuan Liu, Rina Sa, Xiaoyan Zhu, Jihong Hu","doi":"10.1007/s42770-024-01582-w","DOIUrl":"https://doi.org/10.1007/s42770-024-01582-w","url":null,"abstract":"<p><strong>Introduction: </strong>Helicobacter pylori (H. pylori) is a major human pathogen whose increased antibiotic resistance poses a serious threat to human health.</p><p><strong>Aim: </strong>The aim of this study is to further explore the association between H. pylori resistance to clarithromycin, levofloxacin, metronidazole, amoxicillin, rifampicin, tetracycline and its genomic characteristics.</p><p><strong>Methodology: </strong>Using H. pylori isolates, we studied their susceptibility to six antibiotics by the agar dilution method. By performing whole-genome resequencing of the H. pylori genomic DNA, the differences in single nucleotide polymorphisms (SNPs) between phenotype resistant and sensitive strains were statistically analyzed to identify potential mutation sites related to drug resistance, and the consistency between genotype and phenotype resistance was analyzed.</p><p><strong>Results: </strong>The drug resistance rates of 65 H. pylori isolates are as follows: clarithromycin 36.9%, levofloxacin 29.2%, metronidazole 63.1%, amoxicillin 7.7%, rifampicin 12.3%, and tetracycline 3.1%. Based on the whole genome resequencing results of H. pylori isolates, 10 new mutations that may be related to drug resistance were identified. There is strong consistency between the genotype and phenotype resistance of clarithromycin and levofloxacin.</p><p><strong>Conclusion: </strong>The resistance rate to amoxicillin and tetracycline is relatively low in Northern China. and the above two antibiotics can be given priority for clinical treatment. It has a high resistance rate to metronidazole and should be avoided as much as possible, or combined with other drugs for treatment. The 10 mutations identified through analysis that only exist in drug-resistant strains may be associated with levofloxacin, metronidazole, amoxicillin, and rifampicin resistance, respectively. The results indicate that genotype testing of H. pylori can serve as a method for predicting its resistance to clarithromycin and levofloxacin.</p>","PeriodicalId":9090,"journal":{"name":"Brazilian Journal of Microbiology","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2024-12-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142806440","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"On the backward excursions in the free-swimming magnetotactic multicellular prokaryote 'Candidatus Magnetoglobus multicellularis'.","authors":"Carolina N Keim, Marcos Farina","doi":"10.1007/s42770-024-01584-8","DOIUrl":"https://doi.org/10.1007/s42770-024-01584-8","url":null,"abstract":"<p><p>Magnetotactic bacteria align to magnetic field lines while swimming in a behavior known as magnetotaxis. They are diverse phylogenetically and morphologically and include both unicellular and multicellular morphologies. The magnetotactic multicellular prokaryote (MMP) 'Candidatus Magnetoglobus multicellularis' has been extensively studied, even though it remains uncultured up to now. It swims back and forth along magnetic field lines, exhibiting a preferential swimming direction that is usually south-seeking, as described for most magnetotactic microorganisms from the Southern Hemisphere. In order to understand the effects of the magnetic field intensity on the backward excursions of 'Ca. M. multicellularis', we applied magnetic fields ranging from 0.09 to 3.4 mT and recorded their movements. Each microorganism was followed frame by frame generating position coordinates, which were used to calculate the frequency of reversal events, as well as the time, distance, and velocity. The velocities in forward movements before and after backward excursions are similar, but no relation was found with the velocity in backward movements. The shapes of the trajectories are distinct in forward and backward movements. In addition, the backward velocities are usually higher. The sharp changes in direction (approximately 180°) indicate that reversal of the flagella rotation direction is the probable mechanism for swimming backward. In conclusion, the backward excursions provide additional freedom of movement to the microorganism, especially when it is constrained by magnetic fields stronger than the Earth's. Backward movements integrate the 'Ca. M. multicellularis' behavioral toolbox, which includes also negative phototaxis, photokinesis, magnetotaxis and possibly helical klinotaxis.</p>","PeriodicalId":9090,"journal":{"name":"Brazilian Journal of Microbiology","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2024-12-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142805605","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Candida krusei (Pichia kudriavzevii) multilocus sequence typing and antifungal susceptibility profile in Cameroon.","authors":"Cyrille Levis Kountchou, Jihane Kabtani, Aude Ngueguim Dougue, Claude Nangwat, Alfred Itor Ekpo, Marie Chantal Ngonde, Cyrille Bruno Mogo, Jean Paul Dzoyem, Stéphane Ranque, Thierry Kammalac Ngouana","doi":"10.1007/s42770-024-01569-7","DOIUrl":"https://doi.org/10.1007/s42770-024-01569-7","url":null,"abstract":"<p><p>Among the Candida species commonly involved in superficial and more significant life-threatening infections, C. krusei exhibits the most worrisome resistance profile to antifungals. This study aimed to analyse the population structure using multilocus sequence typing (MLST), and to evaluate the antifungal susceptibility profile of C. krusei isolated from patients living with human immunodeficiency virus (HIV) in Cameroon. C. krusei isolated from stool, urine, mouth and vaginal samples were identified using routine laboratory techniques and matrix-assisted laser desorption ionization-time-of-flight mass spectrometry (MALDI-TOF MS). The C. krusei isolates were further analysed by MLST. In vitro antifungal susceptibility testing was performed using the Sensititre Yeast One™ microdilution technique. Forty three (43) C. krusei isolates were included in the study. The MLST identified 32 Diploid sequence type (DST), of which 31 were new that were not included in the current database. New alleles were not observed. Different DSTs were observed in isolates from the same geographical area, from different anatomical sites in the same patient. An eBURST analysis clustered all identified DSTs of former isolates in clonal complex 1. Heterogeneous antifungal MICs were observed in isolates of the same DST and/or from the same geographical area. 32.6% of the isolates displayed a resistant or non-wild-type phenotype to at least 3 distinct antifungal agent classes. The achieved results support the setting up of molecular epidemiology and antifungal resistance surveillance of C. krusei.</p>","PeriodicalId":9090,"journal":{"name":"Brazilian Journal of Microbiology","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2024-12-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142799430","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}