Brazilian Journal of Microbiology最新文献

{"title":"Emergence of bla_OXA-181-bearing tigecycline-resistant Klebsiella aerogenes in China.","authors":"Yinfei Fang, Zhaoxia Wu, Wen Zheng, Hongwei Zhou, Lingna Hu, Qiong Xiao, Lihong Bo, Kai Shen, Yongjun Ma","doi":"10.1007/s42770-024-01524-6","DOIUrl":"https://doi.org/10.1007/s42770-024-01524-6","url":null,"abstract":"<p><p>We report the isolation of a bla_OXA-181-positive, tigecycline-resistant Klebsiella aerogenes strain KA04 from a Chinese inpatient's fecal sample. Species identification was performed using MALDI-TOF MS. The antibiotic susceptibilities were assessed via the broth microdilution method. To elucidate the transmission and genetic structure of the bla_OXA-181 gene, conjugation assays and whole-genome sequencing (WGS) were performed. KA04 displayed resistance to carbapenems, quinolones, piperacillin/tazobactam and tigecycline. Through WGS and conjugation experiments, it was possible to confirm bla_OXA-181 and qnrS1 genes causing antibiotic resistance were located on a 51-kb IncX3 type mobile plasmid, bla_OXA-181 gene could be successfully transferred into E. coli EC600 at a conjugation frequency of 1.1 × 10^- 4. tet(A) gene was located on both the chromosome and non-transmissible IncFIB(K) plasmid. This is a tigecycline-resistant K. aerogenes harboring bla_OXA-181 isolate from human fecal sample, highlighting a significant public health concern. Further comprehensive surveillance is needed.</p>","PeriodicalId":9090,"journal":{"name":"Brazilian Journal of Microbiology","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2024-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142457838","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biotechnological potential of Lacticaseibacillus paracasei Shirota for bioemulsifier, bacteriocin and lipase production.","authors":"Naiany Silva de Medeiros, Felipe Ferreira da Nóbrega, Patricia Santos Lopes, Cristiane Fernandes de Assis, Francisco Canindé de Sousa Júnior","doi":"10.1007/s42770-024-01534-4","DOIUrl":"https://doi.org/10.1007/s42770-024-01534-4","url":null,"abstract":"<p><p>This study aimed to evaluate the biotechnological potential of Lacticaseibacillus paracasei Shirota to produce biosurfactants/bioemulsifiers, lipase, and bacteriocins. The production of biosurfactants/bioemulsifiers was evaluated through a central composite rotational design (CCRD) 2². L. paracasei produced bioemulsifiers using MRS supplemented with 4.8% glycerol and pH 6 or 7. In addition, the culture supernatants of L. paracasei were tested for antioxidant, antidiabetic, and lipolytic activities. The tested supernatants did not exhibit antioxidant activity. On the other hand, they showed inhibitory activity for amyloglucosidase (20.7% and 23.9%) and lipolytic activity (16.12 and 19.00 U/mL). In addition, a CCRD 2³ was performed to evaluate the production of bacteriocins. The peptone and lactose concentration variables, as well as pH positively influenced the production of bacteriocins by L. paracasei. In conclusion, L. paracasei is a viable source of antidiabetic metabolites, bacteriocins, bioemulsifiers, and lipase, suggesting that they are promising to be applied in the pharmaceutical, cosmetic, environmental, food, and biomedical industries.</p>","PeriodicalId":9090,"journal":{"name":"Brazilian Journal of Microbiology","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2024-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142399220","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Five new species of endophytic Penicillium from rubber trees in the Brazilian Amazon.","authors":"Kaliane Sírio Araújo, Janaina Lana Alves, Olinto Liparini Pereira, Marisa Vieira de Queiroz","doi":"10.1007/s42770-024-01478-9","DOIUrl":"https://doi.org/10.1007/s42770-024-01478-9","url":null,"abstract":"<p><p>The Amazon rainforest is the world's most diverse ecosystem, full of fauna and flora. Among the trees that make up the forest are the rubber trees of the genus Hevea (H. brasiliensis and H. guianensis), which stand out for the industrial use of latex. It was previously shown that endophytic fungi colonize the leaves, stems, and roots of Hevea spp. In this study, 47 Penicillium spp. and three Talaromyces spp. isolates were analyzed using specific DNA barcodes: internal transcribed spacers region (ITS), β-tubulin (BenA), calmodulin (CaM), and the DNA-dependent RNA polymerase II second largest subunit (RPB2) genes and additionally, for species delimitation, the genealogical concordance phylogenetic species recognition (GCPSR) criteria were applied. The phylogenetic analyses placed the Penicillium isolates into four sections Lanata-Divaricata, Sclerotiora, Citrina, and Fasciculata. The morphological and molecular characteristics resulted in the discovery of five new species (P. heveae sp. nov., P. acrean sp. nov., P. aquiri sp. nov., P. amazonense sp. nov., and P. pseudomellis sp. nov.). The five new species were also compared to closely related species, with observations on morphologically distinguishing features and colony appearances. Bayesian inference and maximum likelihood analysis have supported the placement of P. heveae sp. nov. as a sister group to P. globosum; P. acrean sp. nov. and P. aquiri sp. nov. as sister groups to P. sumatrense; P. amazonense sp. nov. closely related to isolates of P. rolfsii, and P. pseudomellis sp. nov. closely related to P. mellis. The study of endophytic Penicillium species of rubber trees and the description of five new taxa of Penicillium sect. Citrina, Lanata-Divaricata, and Sclerotiora as endophytes add to the fungal biodiversity knowledge in native rubber trees. Reports of fungi in native tropical plants may reveal taxonomic novelties, potential pathogen control agents, and producers of molecular bioactive compounds of medical and agronomic interest.</p>","PeriodicalId":9090,"journal":{"name":"Brazilian Journal of Microbiology","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2024-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142387916","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genotypic and phenotypic characterization of the first Latin America isolates of Corynebacterium rouxii, a recently described member of the Corynebacterium diphtheriae complex reported in Europe.","authors":"Lincoln de Oliveira Sant'Anna, Louisy Sanches Dos Santos, Juliana Nunes Ramos, Sérgio Bokermann, Mireille Ângela Bernardes Sousa, Fernanda Diniz Prates, Ana Luíza Mattos-Guaraldi, Verônica Viana Vieira, Max Roberto Batista Araújo","doi":"10.1007/s42770-024-01526-4","DOIUrl":"10.1007/s42770-024-01526-4","url":null,"abstract":"<p><p>The genus Corynebacterium is the largest genera among corynebacteria and has a range of species widely spread in ecological niches, some with epidemic potential and capable of causing fatal diseases. In recent years, due to the reclassifications and discoveries of new potentially toxin-producing species, microbiological identification and epidemiological control have been compromised, becoming possible only with sequencing techniques. Two bacterial strains isolated from a cat were identified by MALDI-TOF mass spectrometry as Corynebacterium diphtheriae and sent to the collaborating center of the Brazilian Ministry of Health for molecular identification and determination of toxigenicity potential, which were initially performed by multiplex PCR method. In addition, the antimicrobial susceptibility profile was determined according to BrCAST. Finally, for the final identification at the species level and effective epidemiological monitoring, the sequencing of the 16S rRNA and rpoB housekeeping genes was carried out. The isolates were identified as nontoxigenic C. diphtheriae strains by mPCR. Both strains were found susceptible to all antimicrobial agents. Although the identification at the species level was not possible through similarity analysis of  S rRNA and rpoB housekeeping genes, the phylogenetic analysis showed that the isolates belonged to the species Corynebacterium rouxii with a high value of reliability. This is the first report of the isolation of C. rouxii in Latin America. Molecular identification, whether by the MALDI-TOF mass spectrometry or PCR techniques, does not discriminate C. rouxii from C. diphtheriae, requiring gene sequencing and phylogenetic analysis for correct identification at the species level.</p>","PeriodicalId":9090,"journal":{"name":"Brazilian Journal of Microbiology","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2024-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142379987","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bio-adsorption of Fe (II) by dry biomass of metal-tolerant haloarchaeon Haloferax alexandrinus GUSF-1.","authors":"Sanika Naik-Samant, Sanket Gaonkar, Irene Furtado","doi":"10.1007/s42770-024-01535-3","DOIUrl":"https://doi.org/10.1007/s42770-024-01535-3","url":null,"abstract":"<p><p>Mining-associated activities result in iron pollution exceeding the acceptable limit of 0.3 mg L^- 1 and are rampant in estuarine soil and water bodies that harbor halophilic microorganisms. Biotechnologies are underway to unveil the concentrations and recover the metals that skip existing physico-chemical methods. Concerning this, the present study describes for the first time the development of a bio-adsorption batch system using dried cells of Haloferax alexandrinus GUSF-1 for Fe (II) from saline water under microaerophilic conditions. A maximum of 99.5% Fe (II) was adsorbed at pH 6.0, 30 ºC in 3 h with 92% efficiency over three adsorption-desorption cycles with saturation and pseudo-second-order kinetics and heterogeneity of Freundlich model having K_F of 1.38 mg g^- 1 with the n value of 0.96. Adsorbed Fe (II) by the cells was detected by scanning electron microscopy. The involvement of the carboxyl, amino, hydroxyl, and phosphate groups of the cells in interaction with the metal ions was detected by infrared spectroscopy. Conclusively, the study is the first report of whole dried cells mediated metal adsorption by the haloarcheon Haloferax alexandrinus GUSF-1 which acts as promising candidate for metal clean-up strategy and bioremediation in hypersaline ecosystems.</p>","PeriodicalId":9090,"journal":{"name":"Brazilian Journal of Microbiology","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2024-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142379986","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antibacterial and antibiofilm activity of human gut lactic acid bacteria.","authors":"Sherin Joy Parappilly, E K Radhakrishnan, Sumi Mary George","doi":"10.1007/s42770-024-01530-8","DOIUrl":"https://doi.org/10.1007/s42770-024-01530-8","url":null,"abstract":"<p><p>The present study focused on the antibacterial and antibiofilm activity of novel lactic acid bacterial (LAB) strains isolated from the healthy human volunteers of different age groups and their consortium (LABCON), against the enteropathogenic bacteria. From the study, methanolic extract of LAB isolates and their consortia were found to have promising antibacterial activity and antibiofilm activity against Escherichia coli (ATCC 35218) and Staphylococcus aureus (ATCC 25923). The antimicrobial compounds including the DL-3 phenyllactic acid, DL-p-hydroxyphenyllactic acid, and Succinic acid produced by the LAB could be considered to inhibit the growth and biofilm formation by E. coli (ATCC 35218) and S. aureus (ATCC 25923). Detailed insight into the antibiofilm activity could also be demonstrated by Confocal Raman microscopy attached with AFM and Fluorescent microscope. From the results of the study, the consortium LABCON was superior in antimicrobial and antibiofilm activity and can be considered to have promising application in infection control.</p>","PeriodicalId":9090,"journal":{"name":"Brazilian Journal of Microbiology","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2024-10-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142370957","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The gut microbiota of wild birds undergoing rehabilitation as a reservoir of multidrug-resistant enterococci in a metropolitan area in Brazil.","authors":"Andrea de Andrade Rangel Freitas, Adriana Rocha Faria, Luiza Tomé Mendes, Vânia Lúcia Carreira Merquior, Daniel Marchesi Neves, Jeferson Rocha Pires, Lúcia Martins Teixeira","doi":"10.1007/s42770-024-01527-3","DOIUrl":"https://doi.org/10.1007/s42770-024-01527-3","url":null,"abstract":"<p><p>Enterococci are ubiquitous usually commensal bacteria that can act as opportunistic pathogens frequently associated with resistance to multiple antimicrobial classes. A variety of animals may carry potentially harmful enterococci. In the present work, the occurrence and characteristics of enterococci recovered from the fecal microbiota of wild birds belonging to four families (Accipitridae, Cathartidae, Falconidae and Strigidae) were investigated. Enterococci were recovered from 104 (92.0%) fecal samples obtained from 113 birds, and 260 strains were selected for additional characterization. Enterococcus faecalis was the predominant species (63.8%), followed by Enterococcus hirae (16.2%), Enterococcus faecium (11.5%), Enterococcus gallinarum (5.4%), Enterococcus avium (1.5%), Enterococcus casseliflavus (0.8%), and Enterococcus raffinosus and Enterococcus cecorum (0.4% each). Major percentages (11.9% 75.0%) of nonsusceptibility were observed to quinolones (particularly to enrofloxacin), erythromycin, rifampin, nitrofurantoin, tetracycline and streptomycin. Gentamicin and ampicillin resistances (13.3% each) were only detected among E. faecium. A total of 133 (51.2%) strains were MDR, showing a large variety of MDR profiles, composed by simultaneous resistance encompassing 3 to 12 antimicrobials. MDR strains were found in 68.2% of the birds. Antimicrobial resistance was associated with the presence of the aac(6')-aph(2″)-Ia, aph(2″)-Id, ant(6)-Ia, ant(9)-Ia, ant(9)-Ib, tet(M), tet(L), tet(S), erm(B), mef(A/E), msrC, and vat(D) genes. The most common virulence genes were efaA, gelE, ace, eeP, and asa1. PFGE analysis revealed a large genetic diversity among most of the strains. MLST performed for 35 E. faecalis strains revealed 23 different STs, whereas 14 STs were found among 18 E. faecium strains. Hospital-associated lineages ST22, ST25, ST56, ST1274 were identified. The results show that the wild birds investigated can carry a diversity of potentially hazardous enterococcal strains displaying multiple antimicrobial resistance and virulence genes, reinforcing the assumption that these animals provide an important target to monitor the circulation of microorganisms that deserve consideration under the One Health perspective.</p>","PeriodicalId":9090,"journal":{"name":"Brazilian Journal of Microbiology","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2024-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142360998","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Application of biosurfactants in enhanced oil recovery ex-situ: a review.","authors":"Anny Andrade, Ana Mehl, Eduardo Mach, Paulo Couto, Claudia Regina Elias Mansur","doi":"10.1007/s42770-024-01515-7","DOIUrl":"https://doi.org/10.1007/s42770-024-01515-7","url":null,"abstract":"<p><p>With a growing focus on environmentally friendly solutions, biosurfactants derived from plants or microorganisms have gained attention for Enhanced Oil Recovery (EOR) applications. Biosurfactants offer several advantages over existing options, including biodegradability, low toxicity, availability of raw materials, resistance to harsh reservoir conditions, and improved water/oil interfacial tension reduction. Different organisms, such as bacteria, fungi, and plants, can produce these natural surfactants. Bacillus sp. and Pseudomonas sp. bacteria are extensively studied for their ability to produce biosurfactants using low-cost carbon and nitrogen sources, exhibiting excellent surface activity and low critical micellar concentration (CMC). Fungi, though less commonly used, can also produce biosurfactants, albeit with lower interfacial activity. Plant-derived natural surfactants find wide application in laboratory tests for EOR, despite having higher CMC. This review not only summarizes the current knowledge on biosurfactants but also offers a novel comparative analysis of those produced by bacteria, fungi, and plants, examining their CMC, surface tension, and interfacial tension properties. Additionally, it quantifies the number of publications on the use of biosurfactants for Microbial Enhanced Oil Recovery ex-situ (MEOR ex-situ) over the past 30 years and compares these with biosurfactants derived from plant sources. Our study is unique in its comparative approach and the quantification of literature on MEOR ex-situ. The findings reveal that biosurfactants produced by bacteria generally exhibit superior surface activity, even at lower concentrations, compared to those produced by plants or fungi. This new comparative perspective and thorough literature analysis highlight the distinctive contributions of this study. Overall, the use of biosurfactants for EOR represents a promising approach to cleaner energy production, with the potential to reduce environmental impact while improving oil recovery.</p>","PeriodicalId":9090,"journal":{"name":"Brazilian Journal of Microbiology","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2024-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142360997","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prevalence and molecular characterization of carbapenem-resistant Enterobacterales in patients from a public referral hospital in a non-metropolitan region of Brazil during and post the SARS-CoV-2 pandemic.","authors":"Romário Costa Fochat, Ana Clara de Lelis Araújo, Olavo Dos Santos Pereira Júnior, Marcelo Silva Silvério, Alessandra Figueiredo de Castro Nassar, Maria de Lourdes Junqueira, Marcio Roberto Silva, Patrícia Guedes Garcia","doi":"10.1007/s42770-024-01531-7","DOIUrl":"https://doi.org/10.1007/s42770-024-01531-7","url":null,"abstract":"<p><p>Antimicrobial resistance (AMR) poses a global threat, with carbapenem-resistant Enterobacterales (CRE) representing a significant concern due to limited therapeutic options. This study investigated the prevalence of carbapenemase genes in CRE strains isolated from tracheal aspirates of patients at a Brazilian university hospital between January 2020 and August 2023. Bacterial identification was conducted using MALDI-TOF, while carbapenemase genes were detected by qPCR. Demographic and clinical data were collected, and univariate analysis was performed using the chi-square test (p < 0.05). Variables with p ≤ 0.10 were further investigated using the chi-square test for linear trend, along with stratified analysis. Out of 1,133 samples, 111 (9.79%) showed CRE growth, with 46 isolates included in the final sample, predominantly comprising Klebsiella pneumoniae (65.21%) and Serratia marcescens (19.57%). The bla_KPC gene was prevalent (78.26%), while bla_NDM was detected in 21.74% of cases. The identified population was predominantly male (67.39%), elderly (69.57%), white (56.52%), unmarried (63.04%), and had a low level of education (56.52%). Most patients (69.57%) were in the intensive care unit and remained hospitalized for more than 30 days (76.08%). There was a significant inverse trend between Klebsiella pneumoniae and age (p = 0.045), as well as a direct linear trend between bla_NDM and the annual increase in COVID-19 cases in Brazil (p = 0.050). A high probability of finding non-Klebsiella pneumoniae bacteria was observed in patients with prolonged hospital stays, independent of COVID-19 (p = 0.006) and the type of resistance genes (p = 0.020). The persistent prevalence of CRE, especially with bla_KPC, underscores the urgency of effective control measures.</p>","PeriodicalId":9090,"journal":{"name":"Brazilian Journal of Microbiology","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142341531","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antifungal susceptibility and biofilm production of Candida species- causative agents of female genital tract infections.","authors":"Marina Ranđelović, Marina Dimitrijević, Stefan Mijatović, Aleksandra Ignjatović, Valentina Arsić-Arsenijević, Zorica Stojanović-Radić, Roderick Hay, Suzana Otašević","doi":"10.1007/s42770-024-01529-1","DOIUrl":"https://doi.org/10.1007/s42770-024-01529-1","url":null,"abstract":"<p><strong>Background: </strong>Recurrent vulvovaginal candidosis (RVVC) is a chronic infection affecting 8-10% of women worldwide. Biofilm production of the infecting species and reduced sensitivity to antimycotics could contribute to the recurrence of this infection. This study aimed to examine the biofilm production ability and antifungal susceptibility of genital yeast isolates to determine their virulence potential.</p><p><strong>Methods: </strong>Matrix-assisted laser desorption in ionization-time of flight mass spectrometry (MALDI-TOF MS) was used to identify 300 Candida species. Using crystal violet method, strains were categorized into non-producers, weak, moderate, and strong biofilm producers (BFP). Antifungal susceptibility testing was performed using commercial Integral System YEASTS Plus test (ISYPT) and broth microdilution method (BMM).</p><p><strong>Results: </strong>MALDI-TOF MS identified 150 Candida albicans, 124 non-albicans Candida (NAC), and 26 Saccharomyces cerevisiae strains. Within 138 (46.0%) BFP, 23 (16.7%) were strong, 44 (31.9%) moderate, and 71 (51.4%) weak. BMM was done for 43 BFP selected isolates with nystatin MIC ˃1.25 μl, fluconazole MIC ˃64 μl, and clotrimazole MIC ˃1.0 μl determined by ISYPT. Compared to all examined isolates, BMM confirmed that: i) C. albicans and NAC BFP showed low sensitivity to fluconazole (12% and 4%, respectively); ii) all BFP showed low sensitivity to nystatin (12.7% C. albicans, 14.5% NAC, and 23.1% S. cerevisiae); iii) clotrimazole in vitro was the most efficient regarding C. albicans and S. cerevisiae strains, but in 4.0% NAC BFP for this antimycotic higher MIC was established.</p><p><strong>Conclusion: </strong>Novel antimycotics or possible combinations of antifungal agents and natural products could be a new treatment option for RVVC.</p>","PeriodicalId":9090,"journal":{"name":"Brazilian Journal of Microbiology","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142341527","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}