Brazilian Journal of Microbiology最新文献

{"title":"Biosynthesis in Streptomyces sp. NRRL S-1813 and regulation between oxazolomycin A and A2.","authors":"Qingyun Zhang, Daotong Lv, Huiyu Gong, Jiayi Ren, Yunbin Lyu, Shaochen Wang, Zhiyang Feng","doi":"10.1007/s42770-025-01735-5","DOIUrl":"10.1007/s42770-025-01735-5","url":null,"abstract":"<p><p>A novel actinomycete strain, Streptomyces sp. NRRL S-1813 was employed to study its secondary metabolites under different mediums to activate its cryptic gene clusters and produce antimicrobial secondary metabolites. During fermentation optimization, and purification, oxazolomycin A and oxazolomycin A2 were isolated from one strain simultaneously. Their structure was elucidated using a series of characterization techniques, including full wavelength scanning, mass spectrometry (MS), and nuclear magnetic resonance (NMR) spectroscopy. Oxazolomycin A2 was found not to be a typical enzymatic product of fermentation process. Instead, a spontaneous, non-enzymatic ring cleavage reaction was identified as mechanism for conversion of oxazolomycin A to oxazolomycin A2. Basing on these results, if the target product is oxazolomycin A2, the best fermentation condition of Streptomyces sp. NRRL S-1813 should be the Medium B under the alkalescence condition. For the biosynthesis of oxazolomycin A, the medium pH and reaction time were both important. A slightly acidic environment suppresses the side reactions such as hydrolysis of product, while reasonable reaction time minimizes accumulation of byproducts.</p>","PeriodicalId":9090,"journal":{"name":"Brazilian Journal of Microbiology","volume":" ","pages":"1519-1528"},"PeriodicalIF":1.9,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12350879/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144607311","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corynebacterium pseudodiphtheriticum as etiologic agent of human infections worldwide: a review of case reports within the last 40 years (1983-2023).","authors":"Marcos Andrade-Silva, Louisy Sanches Dos Santos, Ana Luíza Mattos-Guaraldi","doi":"10.1007/s42770-025-01739-1","DOIUrl":"10.1007/s42770-025-01739-1","url":null,"abstract":"<p><p>Corynebacterium pseudodiphtheriticum has been increasingly reported as an etiologic agent of both community-acquired and nosocomial infections, despite its common presence as a harmless member of the human upper respiratory tract and skin microbiota. However, infections caused by C. pseudodiphtheriticum and other corynebacterial species are still frequently misclassified as contamination and overlooked during clinical and laboratory diagnoses in several countries. In this study, an extensive review of human infections and nosocomial outbreaks caused by C. pseudodiphtheriticum was conducted through the analysis of reported cases from various countries, over a 40-year period (1983-2023). This review highlights the dichotomous nature of C. pseudodiphtheriticum, emphasizing both its potential biotechnological applications and its role as a human pathobiont affecting immunocompromised and immunocompetent individuals worldwide. Therefore, this study supports the inclusion of C. pseudodiphtheriticum in the list of clinically relevant pathogens and underscores the need for research into the virulence mechanisms involved in its pathogenesis.</p>","PeriodicalId":9090,"journal":{"name":"Brazilian Journal of Microbiology","volume":" ","pages":"1929-1947"},"PeriodicalIF":1.9,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12350908/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144641801","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Veterinary application of LAMP: a simple and visual detection tool for feline sporotrichosis.","authors":"Lívia do Carmo Silva, Isabela Cristina de Oliveira Dos Santos, Juliana Santana de Curcio, Allana de Paula Castilho, João Vitor Gregolan Barbosa, Luiz Henrique Alves Costa, Álvaro Ferreira Júnior, Elisângela de Paula Silveira-Lacerda","doi":"10.1007/s42770-025-01694-x","DOIUrl":"10.1007/s42770-025-01694-x","url":null,"abstract":"<p><p>Early diagnosis of sporotrichosis, along with the surveillance of fungal circulation in both human and domestic animal populations, is critical for global health. Although the gold standard method is microbiological culture followed by microscopic analysis of the fungus, this methodology requires a long period and skilled professionals for pathogen identification. We developed a molecular assay and conducted a preliminary evaluation using clinical samples using Loop-Mediated Isothermal Amplification (LAMP) technology that detects Sporothrix schenckii and Sporothrix brasiliensis fungi, with results in 30 min. Results are visually interpreted and do not require DNA extraction, making it a promising tool for point-of-care diagnosis in veterinary settings.</p>","PeriodicalId":9090,"journal":{"name":"Brazilian Journal of Microbiology","volume":" ","pages":"1483-1493"},"PeriodicalIF":1.9,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12350994/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144156844","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antimicrobial potential and extracellular metabolite profiling of bichitrapur mangrove sediment derived Micromonospora sp. BSS-D-04 and Streptomyces sp. BSS-D-05 using LC-MS/MS and GC-MS analysis.","authors":"Dipransu Pradhan, Mihir Tanay Das","doi":"10.1007/s42770-025-01710-0","DOIUrl":"10.1007/s42770-025-01710-0","url":null,"abstract":"<p><p>Terrestrial actinomycetes have historically dominated the production of antimicrobial compounds. However, recent investigations into aquatic strains, particularly those from marine habitats, have revealed a vast reservoir of bioactive compounds with potential therapeutic applications. This study focuses on actinomycetes isolated from Bichitrapur Mangrove sediments which creates an extreme habitat for the microorganisms. Six distinct colonies were isolated, and their potential for antimicrobial compound production was explored. The investigation integrates molecular and chemical analyses, employing genetic sequencing for taxonomic identification and gas chromatography-mass spectrometry (GC-MS) and liquid chromatography-mass spectrometry (LC-MS/MS) for compound characterization. The 16S rRNA gene sequences of BSS-D-04 and BSS-D-05 were submitted to the NCBI database, confirming their identities as Micromonospora sp. and Streptomyces sp., respectively. Phylogenetic analysis provided insight into the evolutionary relationships of these isolates. The organic solvent extracts from the selected strains were subjected to GC-MS and LC-MS/MS analyses, revealing many novel bioactive compounds. The identified compounds, documented for their broad-spectrum biological activities, encompassed antimicrobial, antifungal, insecticidal, antioxidative, anti-inflammatory, anticancer, antiviral, and antiparasitic properties. Especially, the study highlighted the higher pharmaceutical potential of Micromonospora sp. compared to Streptomyces sp. which is responsible for the 70% of the total discovered antibiotics. Hence, this comprehensive investigation sets the stage for further exploration and purification of metabolites from these highly potent actinobacterial strains which will help in new drug development and potential applications in the pharmaceutical industry.</p>","PeriodicalId":9090,"journal":{"name":"Brazilian Journal of Microbiology","volume":" ","pages":"1843-1859"},"PeriodicalIF":1.9,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12351003/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144474014","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification of additional probiotic attributes in yeasts isolated from tarhana fermentation.","authors":"Belgin Erdem, Halil İbrahim Kaya, Şener Tulumoğlu, Ebru Coteli, Ömer Şimşek","doi":"10.1007/s42770-025-01728-4","DOIUrl":"10.1007/s42770-025-01728-4","url":null,"abstract":"<p><p>Fermented foods constitute a valuable source of probiotics for both bacteria and yeasts. To date, however, there has been a limited amount of research conducted on Saccharomyces cerevisiae, Kazakhstania servazzi, Kluyveromyces marxianus and Torulaspora delbrueckii isolated from tarhana. The objective of the research is to identify additional probiotic characteristics other than the leavening activity exhibited by yeasts that were previously isolated from tarhana fermentation. In this study, yeasts were subjected to subsequent acid and bile salt tolerance, bile salt hydrolysis, antagonistic activity, aggregation activity (auto-aggregation and co-aggregation), cholesterol assimilation, folate production, biofilm production, and hemolysis activity. S. cerevisiae PCF122, S. cerevisiae PCF107, and S. cerevisiae PCF134 strains grew at pH 2 and 2,5 but remained at pH 3. Except for S. cerevisiae PCF115 and T. delbrueckii PCF150, all yeasts were found to be 0,5% and 1,0% oxalate tolerant. All yeasts hydrolyze oxalate (bile salt), but only S. cerevisiae PCF115 and T. delbrueckii PCF150 produced EPS. Yeasts also exhibited significant amounts of autoaggregation (46-87%). After 24 and 48 h incubation, all strains assimilated cholesterol at rates ranging from 10,4% to 87,5% and 10,6-91%, respectively. The highest folate production was determined at S. cerevisiae PCF108 (56 µg/mL) and the lowest was at S. cerevisiae PCF110 and K. marxianus PFC120 (18 µg/mL). In conclusion, yeasts that existed in tarhana fermentation showed cholesterol assimilation, folate production, and aggregation activity which are additional probiotic attributes that would have consumer health promotion, beside these yeast leaven the tarhana dough.</p>","PeriodicalId":9090,"journal":{"name":"Brazilian Journal of Microbiology","volume":" ","pages":"1765-1773"},"PeriodicalIF":1.9,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12350889/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144590406","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fungal diversity present in ornithogenic soils of extreme equatorial Atlantic São Pedro and São Paulo archipelago using DNA metabarcoding.","authors":"Vívian Nicolau Gonçalves, Fábio Oliveira Soares, Guilherme Resende Corrêa, Eduardo Osório Senra, Fabyano A C Lopes, Micheline C Silva, Peter Convey, Paulo E A S Câmara, Alysson Wagner Fernandes Duarte, Luiz Henrique Rosa","doi":"10.1007/s42770-025-01698-7","DOIUrl":"10.1007/s42770-025-01698-7","url":null,"abstract":"<p><p>We evaluated the fungal diversity present in ornithogenically-influenced soils of the polyextreme Brazilian Archipelago of São Pedro and São Paulo, using a DNA metabarcoding approach. We detected 1,596,919 fungal DNA reads grouped into 232 amplicon sequence variants (ASVs). The phylum Ascomycota was the dominant phylum, followed by Basidiomycota, Blastocladiomycota, Mortierellomycota, Mucoromycota, Aphelidiomycota, Rozellomycota, Chytridiomycota and Zoopagomycota, in rank abundance order. Cladosporium sp., Hortaea werneckii, Penicillium sp., Aspergillus sp. and Fungal sp. were the dominant assigned taxa. The fungal assemblages displayed high diversity indices, although differing between the sites sampled. Fifty-four fungal ASVs could only be assigned to higher taxonomic levels, primarily those of the cryptic and poorly known phyla Aphelidiomycota, Blastocladiomycota, Chytridiomycota and Rozellomycota. These may represent taxa not currently included in the available databases or new taxa and/or new records for the São Pedro and São Paulo archipelago. Only Cladosporium sp., Hortaea werneckii, Blastobotrys serpentis and Penicillium sp. were detected in all sites. Even though the São Pedro and São Paulo archipelago experiences extreme environmental conditions, the use of metabarcoding revealed a diverse and complex fungal community potentially present in the samples examined. The assigned fungal communities were dominated by genera which commonly display high adaptive plasticity when facing challenging and extreme conditions. The melanized yeast H. werneckii, known for its high resistance to polyextreme conditions, illustrates that this archipelago represents a potential source of extremophilic fungi. The dominant assigned fungi include taxa with different ecological roles, with some also recognized as potentially important animal pathogens.</p>","PeriodicalId":9090,"journal":{"name":"Brazilian Journal of Microbiology","volume":" ","pages":"1619-1629"},"PeriodicalIF":1.9,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12350997/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144172124","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antimicrobial susceptibility and genetic profiles of Escherichia coli isolated from poultry hatcheries in the state of Rio Grande do Sul, Brazil.","authors":"Jônatas Grellmann Breunig, Kelly Cristina Tagliari de Brito, Tamara Flores, Thales Quedi Furian, Karen Apellanis Borges, Hamilton Luiz de Souza Moraes, Carlos Tadeu Pippi Salle, Luciana Kazue Otutumi, Luís Eduardo de Souza Gazal, Gerson Nakazato, Vanessa Lumi Koga, Renata Katsuko Takayama Kobayashi, Camila de Cuffa Matusaiki, Gabriela Rocha Santos, Benito Guimarães de Brito","doi":"10.1007/s42770-025-01688-9","DOIUrl":"10.1007/s42770-025-01688-9","url":null,"abstract":"<p><p>Escherichia coli is widely distributed in human and animals as part of commensal microbiota, but some isolates can act as major pathogens causing severe diseases. Data regarding the characterization of E. coli isolated from hatchery is limited. Extensive genetic similarity among isolates has been previously reported, including similar virulence and antimicrobial resistance genes, which make it difficult to differentiate between pathogenic and commensal bacteria. The aim of this study was to characterize 84 E. coli isolates from four hatcheries in Rio Grande do Sul state (Brazil) for antimicrobial susceptibility, detection of multidrug resistance (MDR) and extended-spectrum β-lactamases (ESBL) isolates, and phylogenetic groups. Most antimicrobial agents presented moderate (20-50%) or high (> 50%) resistance rates, and 58 phenotypic resistance profiles were observed. A total of 51.19% and 20.24% of isolates were classified as MDR and ESBL, respectively. The majority of the isolates (80.95%) were classified in the pathogenic phylogroups (B2 and D), regardless the hatchery establishment. The hatchery did not influence in any of the results. The results demonstrate the high antibiotic resistance demonstrated by E. coli isolated from hatcheries in southern Brazil. The dissemination of pathogenic isolates in all establishments evaluated is a matter of particular concern. In this context, the results found in this study are important and may be the first step on monitoring pathogenicity and antimicrobial resistance fluctuation in Brazilian hatcheries.</p>","PeriodicalId":9090,"journal":{"name":"Brazilian Journal of Microbiology","volume":" ","pages":"2155-2162"},"PeriodicalIF":1.9,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12350989/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144172753","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Simultaneous mitigation of ochratoxin A and zearalenone by Amano lipase A: conditions and application.","authors":"Sabrina de Oliveira Garcia, Ana Flávia Vendramin Comunello, Diean Fabiano Alvares Pinheiro, Karen Vanessa Marimón-Sibaja, Wesclen Vilar Nogueira, Jaqueline Garda-Buffon","doi":"10.1007/s42770-025-01679-w","DOIUrl":"10.1007/s42770-025-01679-w","url":null,"abstract":"<p><p>Mycotoxins such as ochratoxin A (OTA) and zearalenone (ZEA) are secondary metabolites produced by fungi that exhibit high toxicity and are frequently detected in food and beverages, including beer, the third most consumed beverage worldwide, posing a significant public health concern. The mitigation of these contaminants has become an increasingly urgent priority, particularly in the face of climate change, which is expected to exacerbate their prevalence and concentration throughout the food supply chain. In this context, the development of effective, safe, and food-compatible strategies for reducing mycotoxin levels in complex food matrices is essential to ensure both food quality and consumer safety. Accordingly, this study aimed to evaluate the action of the enzyme Amano lipase A (ALA) in the simultaneous mitigation of OTA and ZEA in model solution and Pilsen type beer. The reaction and kinetic parameters (K_M and V_max) were optimized for this. The application of the enzyme (0.3 U mL^-1) in the mitigation of OTA and ZEA in beer was evaluated. Under optimal reaction conditions to ALA in model solution, consisting of 50 mM pH 7 phosphate buffer, 40 ºC and 22 h of incubation, it simultaneously degraded OTA and ZEA by up to 100.0 and 30.6%, respectively. The kinetic parameters K_M and V_max of ALA in the mitigation of OTA and ZEA were 0.03 and 3.14 µM and 6.56 × 10^-05 and 19.57 × 10^-03 μM min^-1, respectively. The enzyme degraded 89.5% OTA and 6.5% ZEA. The enzyme ALA presents as an alternative for controlling these contaminants in beer or food.</p>","PeriodicalId":9090,"journal":{"name":"Brazilian Journal of Microbiology","volume":" ","pages":"1681-1694"},"PeriodicalIF":1.9,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12350875/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143980318","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Respiratory virus circulation during pandemic: Why were some viruses still circulating?","authors":"Eva Maria de Assis Carvalho, Katia Ozanic, Alessandra Ferreira Barbosa Machado, Vanessa Cordeiro Dias, Claudio Galuppo Diniz, Vania Lucia da Silva, Nancy Bellei, Aripuanã Watanabe","doi":"10.1007/s42770-025-01681-2","DOIUrl":"10.1007/s42770-025-01681-2","url":null,"abstract":"<p><p>A few months after the beginning of the coronavirus disease 2019 (COVID-19) pandemic in March 2020, several non-pharmacological measures were adopted worldwide, with varying degrees of strictness, to contain the transmission of the virus and mitigate its impacts. These measures, in addition to effectively reducing the circulation of SARS-CoV-2 (Severe Acute Respiratory Syndrome Coronavirus 2), these measures also appeared to impact the circulation of other respiratory viruses. Therefore, this study aims to discuss the most relevant data available regarding the circulation of the major respiratory viruses during the COVID-19 pandemic, exploring the factors that allowed some viruses to continue circulating while others experienced a decline. Several authors report that the detection of influenza, respiratory syncytial virus (RSV), human coronaviruses (hCoVs), human parainfluenza viruses (HPIVs), and human metapneumovirus (hMPV) dropped significantly. However, non-enveloped viruses such as adenovirus, and especially human rhinovirus (HRV), did not seem to be as affected. Hypotheses for this scenario include adopting of non-pharmacological measures to curb the spread of COVID-19, behavioral changes in hygiene habits, intrinsic characteristics of each virus such as transmission mode, the presence or absence of a viral envelope and viral interference. Rhinovirus is particularly intriguing, as it maintained a high prevalence during the years of the pandemic. Further investigation into the possible explanations for this phenomenon may be worthwhile.</p>","PeriodicalId":9090,"journal":{"name":"Brazilian Journal of Microbiology","volume":" ","pages":"1913-1928"},"PeriodicalIF":1.9,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12350972/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143971796","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Gene cloning, phenol-responsive transcriptional profiling and recombinant protein characterization of phenol hydroxylase in Candida tropicalis GY8.","authors":"Jianhua Lv, Xiangnan Gu, Minmin Hui, Yingjie Luo, Wenbo Luo, Huiting Zhao, Shuqin Yin, Yan Feng, Zhiquan Xue","doi":"10.1007/s42770-025-01685-y","DOIUrl":"10.1007/s42770-025-01685-y","url":null,"abstract":"<p><p>Phenol hydroxylase (PH) plays a central role in phenol biodegradation pathways. In this study, the enzymatic activity of PH in Candida tropicalis GY8 was evaluated following phenol-induced cultivation, and oxidation products were analyzed via high-performance liquid chromatography (HPLC). Two phenol hydroxylase genes, CtPHE1 and CtPHE2, were identified, and their expression patterns were examined using RT-qPCR. Functional analysis of the corresponding proteins was conducted by cloning and expressing the genes in Escherichia coli BL21(DE3). Enzymatic activity reached its maximum after 15 h of cultivation (p < 0.01), and phenol conversion to catechol was verified by HPLC. RT-qPCR results indicated peak transcript levels at 10 h, with CtPHE2 showing consistently higher expression than CtPHE1 at 5, 10, and 15 h (p < 0.01). Both recombinant proteins were capable of phenol degradation, with CtPHE2 exhibiting greater catalytic activity. These findings provide essential molecular information for advancing the understanding of phenol biodegradation mechanisms.</p>","PeriodicalId":9090,"journal":{"name":"Brazilian Journal of Microbiology","volume":" ","pages":"1985-1995"},"PeriodicalIF":1.9,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12350870/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144198190","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}