Brazilian Journal of Microbiology最新文献

{"title":"Genetic diversity, drug resistance, and biofilm formation in Klebsiella pneumoniae associated with nosocomial infection in Pelotas, RS, Brazil.","authors":"Daniela R Wozeak, Isabel L Pereira, Thayná L Cardoso, Amilton C P S Neto, Daiane D Hartwig","doi":"10.1007/s42770-024-01570-0","DOIUrl":"https://doi.org/10.1007/s42770-024-01570-0","url":null,"abstract":"<p><p>Antibiotic resistance and the potential persistence of Klebsiella pneumoniae strains in hospital environments is an important challenge for human medicine. This research aims to detect resistance to antibiotics, biofilm formation, and the genetic pattern among clinical isolates associated with nosocomial infection obtained from a university hospital in the city of Pelotas, RS, Brazil. Twenty-eight isolates were identified at the species level by polymerase chain reaction (PCR) and were characterized regarding the profile of biofilm formation and antibiotic resistance. The genetic relationship was determined through pulsed-field gel electrophoresis (PFGE). The antibiotic resistance profile was made following the standards established by CLSI. All clinical isolates included in this study were confirmed as belonging to the species K. pneumoniae, 96.42% were considered strong biofilm formers and all were positive in the Congo Red agar (CRA) test. Thus, 64.29% of isolates were classified as multidrug-resistant (MDR), 25% as extensively drug-resistant (XDR), and 7.14% as pandrug-resistant (PDR). PFGE fingerprint analysis revealed 18 clones and of these, 15 have a unique pattern and another three were groups with patterns > 80% similarity. The clinical isolates used were collected over two years and revealed a genetic relationship. The same clone was identified in different types of samples and different years, demonstrating the permanence of the strain in the hospital environment. Our results reaffirm the need for greater measures of control and disinfection within the hospital environment, and the priority of therapeutic measures to contain the propagation of K. pneumoniae.</p>","PeriodicalId":9090,"journal":{"name":"Brazilian Journal of Microbiology","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2024-12-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142791079","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rapid detection of the phytopathogenic oomycete Phytopythium helicoides with a visualized loop-mediated isothermal amplification assay.","authors":"Le-Yao Ren, Ke Wen, Bao-Ping Cheng, Jing-Hao Jin, Vaibhav Srivastava, Xiao-Ren Chen","doi":"10.1007/s42770-024-01578-6","DOIUrl":"https://doi.org/10.1007/s42770-024-01578-6","url":null,"abstract":"<p><p>The phytopathogenic oomycete Phytopythium helicoides, previously known as Pythium helicoides, has emerged as a new threat to the Shatangju citrus cultivar (Citrus reticulata cv. Shatangju; '' in Chinese) in southern China. To enable rapid diagnosis and control of the leaf blight and stem rot caused by P. helicoides, we developed a visualized loop-mediated isothermal amplification (LAMP) assay targeting the β-tubulin gene of the pathogen. Using a carefully selected set of primers and optimized reaction conditions at 60 °C for 60 min, the LAMP assay can specifically detect the pathogen among various citrus-associated phytopathogens. The assay demonstrated high sensitivity, detecting as little as 30 pg of the pathogen DNA, comparable to the sensitivity of quantitative polymerase chain reaction (qPCR). We validated the practical application of this LAMP assay for diagnosing infections in citrus leaves and strawberry crowns. This newly developed LAMP assay offers a specific, sensitive, and rapid detection tool for P. helicoides, aiding in the control of Shatangju citrus leaf blight and stem rot, as well as other related crop diseases like strawberry crown rot.</p>","PeriodicalId":9090,"journal":{"name":"Brazilian Journal of Microbiology","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2024-12-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142791082","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Coinfection with Dengue virus (DENV) serotypes 1 and 2 during a dengue outbreak in the state of Paraná, Brazil.","authors":"Léo Shigueki Sato, Deborah de Castro Moreira, Sonia Kaori Miyamoto, Luciana Dias Ghiraldi Lopes, Dennis Armando Bertolini","doi":"10.1007/s42770-024-01580-y","DOIUrl":"https://doi.org/10.1007/s42770-024-01580-y","url":null,"abstract":"","PeriodicalId":9090,"journal":{"name":"Brazilian Journal of Microbiology","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2024-12-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142784035","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of COVID-19 pandemic on antimicrobial resistance of Proteus mirabilis in a Brazilian hospital.","authors":"Luana Karolyne Salomão de Almeida, Luana Carvalho Silva, Gustavo Henrique Migliorini Guidone, Bruno Henrique Dias de Oliva, Arthur Bossi do Nascimento, Gabriela Faustino, Julia da Silva Pimenta, Eliana Carolina Vespero, Sergio Paulo Dejato Rocha","doi":"10.1007/s42770-024-01568-8","DOIUrl":"https://doi.org/10.1007/s42770-024-01568-8","url":null,"abstract":"<p><p>This study analyzes the resistance and virulence profiles of Proteus mirabilis isolates obtained from patients admitted to the University Hospital of Londrina, Paraná, between 2019 and 2022. We evaluated the antimicrobial resistance phenotypes, genes associated with resistance, biofilm formation through a phenotypic assay, and the presence of specific virulence genes. When comparing the \"pre-pandemic\" (2019) and \"pandemic\" (2020-2022) periods, we observed an increase in resistance rates to all tested antimicrobials. Multidrug-resistant (MDR) pathogens producing extended-spectrum β-lactamase (ESBL) phenotypes were isolated in both periods, but their occurrence was significantly higher during the pandemic. We also observed an increase in the frequency of nearly all studied resistance genes. The virulence profiles remained largely unchanged. Analysis of patients' clinical and demographic data revealed that those hospitalized during the pandemic were older, required longer hospital stays, and had a higher usage of invasive devices. These findings suggest that the recent COVID-19 pandemic has impacted the antimicrobial resistance of P. mirabilis, a bacterium of significant clinical interest associated with urinary tract infections (UTIs) and healthcare-associated infections (HAIs).</p>","PeriodicalId":9090,"journal":{"name":"Brazilian Journal of Microbiology","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2024-12-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142766166","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biodegradation of various edible oils and fat by Staphylococcus petrasii sub sp. jettensis VSJK R1 for application in bioremediation of lipid rich restaurant wastewater.","authors":"Vinayak P Sutar, Varsha K Singh, Rajeshwar P Sinha","doi":"10.1007/s42770-024-01577-7","DOIUrl":"https://doi.org/10.1007/s42770-024-01577-7","url":null,"abstract":"<p><p>The disposal of fat, oil, and grease (FOG) pollutants from various sources, including restaurants, food processing facilities, and domestic kitchens, poses significant challenges to wastewater treatment systems. In this study, we isolated and characterized a novel bacterial strain. The result of 16 S rRNA gene and phylogenetic analysis showed that the isolate was Staphylococcus petrasii sub sp. jettensis and named as VSJK R1 (Fig. S1). It was tested for its biodegradation potential of FOG contaminants. Our investigation revealed that Staphylococcus petrasii sub sp. jettensis VSJK R1 effectively degraded a variety of edible oils, including soybean, sunflower, cottonseed, palm, groundnut, and butter, with notable efficiency. Optimization studies were conducted to determine the optimal conditions for biodegradation, including the effects of nitrogen, carbon, phosphorus, pH, temperature, and salt concentration. Results indicated that organic nitrogen sources and glucose as carbon source significantly enhanced biodegradation rates, while the addition of phosphorus further improved degradation efficiency within specific concentration ranges. Moreover, the optimal pH for biodegradation was found to be neutral, with temperature ranging between 22°C and 45°C favoring microbial activity. Remarkably, Staphylococcus petrasii sub sp. jettensis VSJK R1 exhibited resilience to high salt concentrations, making it suitable for treatment of wastewater with elevated salt content. Additionally, comparative studies with other microbial strains underscored the unique biodegradation capabilities of Staphylococcus petrasii sub sp. jettensis VSJK R1, particularly in degrading various edible oils. The results suggest promising applications of this novel isolate in bioremediation efforts targeting FOG pollutants in wastewater treatment plants and grease traps. Future research may focus on scaling up the bioremediation process and field testing the efficacy of Staphylococcus petrasii sub sp. jettensis VSJK R1 in real-world wastewater treatment scenarios.</p>","PeriodicalId":9090,"journal":{"name":"Brazilian Journal of Microbiology","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2024-12-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142766086","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring ultraviolet and nitrous acid-induced mutagenesis of Rhizopus oligosporus for extracellular inulinase production under solid-state culture.","authors":"Syeda Wajiha Khalid, Sikander Ali, Sadaf Mutahir, Muhammad Asim Khan, Abdulrahman A Almehizia, Jahangir Khan, Amna Waseem","doi":"10.1007/s42770-024-01571-z","DOIUrl":"https://doi.org/10.1007/s42770-024-01571-z","url":null,"abstract":"<p><p>This study details the synthesis and optimization of extracellular inulinase through solid-state fermentation using improved strain of Rhizopus oligosporus. The wild-type was procured from IIB culture bank and subsequently enhanced through UV-radiation and Nitrous acid treatments. The resulting mutant strain was subjected to further optimization for heightened enzyme production via solid-state fermentation. The substrate, pressmud (20 g), and moisture content (20 mL molasses) were carefully selected. Key parameters such as inoculum size, incubation time, and inulin concentration were optimized to maximize enzyme yield. Mutation procedures involved optimizing spore suspension, UV-radiation exposure duration, and the distance of culture plate from UV rays. Nitrous acid treatment, followed by resistance development using L-cysteine HCl, further refined the mutant strains. Evaluation through UV-Vis spectrophotometry and comprehensive characterization using SEM, FTIR, and XRD were conducted to compare the results between the wild-type and mutant strains. The mutant strain exhibited significantly higher inulinase activity (111 ± 0.49 U/ml) as compared to its wild-type counterpart (59 ± 0.65 U/ml), indicating successful enhancement through the applied mutation techniques. The molecular weight of the inulinase enzyme produced from mutant strain and wild-type was 83 and 86 kDa respectively. These findings suggest potential industrial applications of the improved strain in various biotechnological processes.</p>","PeriodicalId":9090,"journal":{"name":"Brazilian Journal of Microbiology","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2024-12-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142766090","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Macaw palm is a harbor of new non-grass species of dark septate endophytes belonging to the genus Pseudophialophora (Magnaporthaceae).","authors":"Jaqueline Aparecida de Oliveira, Fábio Alex Custódio, Olinto Liparini Pereira","doi":"10.1007/s42770-024-01579-5","DOIUrl":"https://doi.org/10.1007/s42770-024-01579-5","url":null,"abstract":"<p><p>Macaw palm (Acrocomia aculeata) is an oleaginous crop native to Brazil with significant economic and environmental value. It has been explored commercially in Brazil within sustainable management. The microbiota associated with macaw palm is still little known and there is no report about their root's fungal endophytes. Recently, the community of dark septate endophytes (DSE) from macaw palm began to be investigated, and new taxa were found. The present study aimed to describe two new Pseudophialophora species associated with macaw palm roots. The isolation method performed was the dilution-to-extinction cultivation. ITS, RPB1, tef1-α, LSU, and SSU regions were amplified and sequenced for phylogenetic analyses. The isolates formed two phylogenetically independent lineages and we proposed the new species P. chlamydospora and P. endophytica. For the first time, new taxa of DSE fungi were described from macaw palm.</p>","PeriodicalId":9090,"journal":{"name":"Brazilian Journal of Microbiology","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2024-12-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142766168","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular detection of Chlamydiales organisms in ruminant abortions in Brazil.","authors":"Ana Paula Vieira Pinto, Wanderson Sirley Reis Teixeira, Camila Dantas Malossi, Fábio Sossai Possebon, Luan Cleber Henker, Marina Paula Lorenzett, Saulo Petinatti Pavarini, David Driemeier, Renata Assis Casagrande, João Pessoa Araújo Júnior, Jane Megid, Mateus Souza Ribeiro de Mioni","doi":"10.1007/s42770-024-01574-w","DOIUrl":"https://doi.org/10.1007/s42770-024-01574-w","url":null,"abstract":"<p><p>Infections caused by the Chlamydiales order are highly prevalent worldwide and have a well-known association with reproductive and non-reproductive disorders in livestock and humans. This study aimed to identify Chlamydiales organisms in ruminant abortions using real-time polymerase chain reaction (qPCR) in samples collected from three Brazilian states: Rio Grande do Sul, Santa Catarina, and São Paulo. The Chlamydiales order was found in 7.8% (18/231) of the samples, including 10 bovine and 8 ovine samples. For the Chlamydiaceae family, the positivity was 2.2% (5/231), with detection in one bovine and two ovine fetuses from Rio Grande do Sul, one ovine fetus from Santa Catarina, and one bovine from São Paulo. Chlamydiales positive samples detection with Ct value lower than 35 were subjected to Sanger sequencing and phylogenetic analysis, which revealed the presence of an organism belonging to the Waddliaceae family in a bovine fetus and two Simkaneceae bacteria in ovine fetuses. This study is the first report of Chlamydia-like organisms in ruminant abortions in Brazil, highlighting the importance of conducting further surveys to evaluate their role as abortifacient pathogens in the country.</p>","PeriodicalId":9090,"journal":{"name":"Brazilian Journal of Microbiology","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2024-12-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142766170","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of Resveratrol on the intestinal microbiota in Type2 diabetes Mellitus mice.","authors":"Hao Liu, Shumin Wang, Xin Yu, Shuguang Sun, Yingzhen Su","doi":"10.1007/s42770-024-01573-x","DOIUrl":"https://doi.org/10.1007/s42770-024-01573-x","url":null,"abstract":"<p><p>Some natural products have been found to have a positive effect on the treatment of Type 2 Diabetes Mellitus (T2DM). The effective mechanism by which these natural products work may be attributed to their ability to alter the gut microbiome. In this study, considering the beneficial properties of resveratrol for T2DM, the mechanism of resveratrol in releasing of T2DM, the correlation between the apparent traits of resveratrol in T2DM mice and the changes in intestinal flora structure were explored. The intervention of resveratrol in different dose did effectively improve the apparent T2DM symptoms in mice, including reduced fasting blood glucose, enhance to both the insulin tolerance and glucose tolerance. Simultaneously, resveratrol can effectively ameliorate the pathological condition of the colon and rectum in T2DM mice, leading to a reduction in intestinal villi swelling and fat vacuoles. The intervention of resveratrol led to changes in the gut flora structure of T2DM mice. Erysipelas and Ileibacterium are crucial taxa of resveratrol involved in maintaining the balance of the gut microbiota. The abundance of Erysipelotrichaceae and Ileibacterium was significantly elevated under the treatment of resveratrol, and the compose of the gut microbiota was more inclined to healthy mice. T2DM symptoms could be alleviated by resveratrol through different mechanisms compared to metformin intervention.</p>","PeriodicalId":9090,"journal":{"name":"Brazilian Journal of Microbiology","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2024-12-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142766089","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Study on the early diagnostic value of nanopore sequencing in alveolar lavage fluid smear-negative pulmonary tuberculosis.","authors":"Yangjing Ou, Dan Li, Xi Long, Huiyang He, Ling Qing, Yuqiu Tian, Jing Ren, Qing Zhou, Yingzheng Tan","doi":"10.1007/s42770-024-01575-9","DOIUrl":"https://doi.org/10.1007/s42770-024-01575-9","url":null,"abstract":"<p><p>The objective of this study was to investigate the early diagnostic value of nanopore sequencing in alveolar lavage smear-negative pulmonary tuberculosis (PTB). A prospective study was conducted on patients hospitalized at Zhuzhou Central Hospital from October 2021 to June 2022 and suspected to have PTB. Alveolar lavage fluid specimens were collected from these patients and simultaneously subjected to centrifugal bacterial collection smear method in a sandwich cup, bifidobacteria solid culture (referred to as culture), Mycobacterium tuberculosis-DNA (TB-DNA), and nanopore sequencing for detection of Mycobacterium tuberculosis. These patients were ultimately diagnosed with smear-negative PTB. The final clinical diagnosis was used as a reference to compare the diagnostic efficacy of Nanopore sequencing, culture, and TB-DNA for PTB. The results showed that the positive rates detected by nanopore sequencing, culture, and TB-DNA in the 103 suspected tuberculosis patients were 73.8%, 13.6%, and 33.0%, respectively. The sensitivity of nanopore sequencing was significantly higher than culture (P < 0.001) and TB-DNA (P < 0.001) for the identification of smear-negative PTB. Similarly, the positive rate of TB-DNA was also significantly higher than that of culture (P = 0.001). In conclusion, nanopore sequencing exhibited the highest sensitivity for the rapid diagnosis of Mycobacterium tuberculosis in alveolar lavage fluid specimens, using clinical diagnosis as a reference standard, and it could improve the PTB clinical diagnosis.</p>","PeriodicalId":9090,"journal":{"name":"Brazilian Journal of Microbiology","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2024-12-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142766171","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}