多药耐药肺炎克雷伯菌的发病率及药敏分析

IF 1.9 4区 生物学 Q3 MICROBIOLOGY
Brazilian Journal of Microbiology Pub Date : 2025-09-01 Epub Date: 2025-05-02 DOI:10.1007/s42770-025-01684-z
Olayinka Remilekun Anifowose, Gbolahanmi Akinola Oladosu, Olayinka Ayotunde Oridupa
{"title":"多药耐药肺炎克雷伯菌的发病率及药敏分析","authors":"Olayinka Remilekun Anifowose, Gbolahanmi Akinola Oladosu, Olayinka Ayotunde Oridupa","doi":"10.1007/s42770-025-01684-z","DOIUrl":null,"url":null,"abstract":"<p><p>Klebsiella pneumoniae is associated with substantial economic losses in the fish industry and with food-borne illness in humans. This study aimed to determine the incidence and antibiogram profiles of multi-drug resistant Klebsiella pneumoniae strains isolated from diseased Clarias gariepinus. A total of 384 diseased Clarias gariepinus that showed clinical signs of red spots and hemorrhages on the pectoral fin were sampled from commercial fish farms in Lagos, Ogun, and Oyo States, Nigeria. Samples of fin, liver, kidney, and gill were taken from diseased C. gariepinus. Samples were subjected to bacteriological examinations and isolates were identified based on morphological characteristics, biochemical tests, and 16 S rRNA gene characterisation. The retrieved isolates were tested for sequence analysis and antibiogram profile. The incidence of K. pneumoniae among the examined diseased Clarias gariepinus was 61.7% (237/384). The highest prevalence was observed in the gill (32.9%). The percentage of K. Pneumoniae isolates in diseased C. gariepinus from selected Southwest states were; Lagos State 46.8%, Ogun State 78.1%, and Oyo State 60.1% (p > 0.05). The phylogenetic analyses revealed that the tested K. pneumoniae strains shared high genetic similarity with other K. pneumoniae strains from South Africa, Saudi Arabia, India, USA, Spain, Bangladesh, and China. Antibiogram profiles revealed that 100% (120/120) of the retrieved K. pneumoniae strains were MDR to four different antimicrobial classes (penicillins, tetracyclines, nitroimidazole, and macrolides). This is the first report to reveal the occurrence of MDR K. pneumoniae in diseased C. gariepinus, an emergence that represents a risk to public health.</p>","PeriodicalId":9090,"journal":{"name":"Brazilian Journal of Microbiology","volume":" ","pages":"1997-2006"},"PeriodicalIF":1.9000,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12350899/pdf/","citationCount":"0","resultStr":"{\"title\":\"Incidence and antimicrobial susceptibility profiles of multi-drug resistant Klebsiella pneumoniae strains isolated from diseased Clarias gariepinus.\",\"authors\":\"Olayinka Remilekun Anifowose, Gbolahanmi Akinola Oladosu, Olayinka Ayotunde Oridupa\",\"doi\":\"10.1007/s42770-025-01684-z\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Klebsiella pneumoniae is associated with substantial economic losses in the fish industry and with food-borne illness in humans. This study aimed to determine the incidence and antibiogram profiles of multi-drug resistant Klebsiella pneumoniae strains isolated from diseased Clarias gariepinus. A total of 384 diseased Clarias gariepinus that showed clinical signs of red spots and hemorrhages on the pectoral fin were sampled from commercial fish farms in Lagos, Ogun, and Oyo States, Nigeria. Samples of fin, liver, kidney, and gill were taken from diseased C. gariepinus. Samples were subjected to bacteriological examinations and isolates were identified based on morphological characteristics, biochemical tests, and 16 S rRNA gene characterisation. The retrieved isolates were tested for sequence analysis and antibiogram profile. The incidence of K. pneumoniae among the examined diseased Clarias gariepinus was 61.7% (237/384). The highest prevalence was observed in the gill (32.9%). The percentage of K. Pneumoniae isolates in diseased C. gariepinus from selected Southwest states were; Lagos State 46.8%, Ogun State 78.1%, and Oyo State 60.1% (p > 0.05). The phylogenetic analyses revealed that the tested K. pneumoniae strains shared high genetic similarity with other K. pneumoniae strains from South Africa, Saudi Arabia, India, USA, Spain, Bangladesh, and China. Antibiogram profiles revealed that 100% (120/120) of the retrieved K. pneumoniae strains were MDR to four different antimicrobial classes (penicillins, tetracyclines, nitroimidazole, and macrolides). This is the first report to reveal the occurrence of MDR K. pneumoniae in diseased C. gariepinus, an emergence that represents a risk to public health.</p>\",\"PeriodicalId\":9090,\"journal\":{\"name\":\"Brazilian Journal of Microbiology\",\"volume\":\" \",\"pages\":\"1997-2006\"},\"PeriodicalIF\":1.9000,\"publicationDate\":\"2025-09-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12350899/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Brazilian Journal of Microbiology\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://doi.org/10.1007/s42770-025-01684-z\",\"RegionNum\":4,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2025/5/2 0:00:00\",\"PubModel\":\"Epub\",\"JCR\":\"Q3\",\"JCRName\":\"MICROBIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Brazilian Journal of Microbiology","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1007/s42770-025-01684-z","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/5/2 0:00:00","PubModel":"Epub","JCR":"Q3","JCRName":"MICROBIOLOGY","Score":null,"Total":0}
引用次数: 0

摘要

肺炎克雷伯菌与养鱼业的重大经济损失和人类食源性疾病有关。本研究旨在确定从患病的加里平克拉虫中分离出的多重耐药肺炎克雷伯菌的发病率和抗生素谱。从尼日利亚拉各斯、奥贡州和奥约州的商业养鱼场共取样384只患病的加里平克拉丽鱼,其临床症状为胸鳍出现红斑和出血。从患病的加里滨棘鱼身上取鳍、肝、肾和鳃标本。对样品进行细菌学检查,并根据形态学特征、生化试验和16s rRNA基因特征鉴定分离株。对检索到的分离株进行序列分析和抗生素谱分析。肺炎克雷伯菌在患病的加里宾克拉虫中的发病率为61.7%(237/384)。鳃部患病率最高(32.9%)。西南各州的肺炎克雷伯菌分离株在患病的加里宾支原体中所占的比例为;Lagos州46.8%,Ogun州78.1%,Oyo州60.1% (p > 0.05)。系统发育分析显示,检测的肺炎克雷伯菌菌株与来自南非、沙特阿拉伯、印度、美国、西班牙、孟加拉国和中国的其他肺炎克雷伯菌菌株具有高度的遗传相似性。抗生素谱显示,100%(120/120)的肺炎克雷伯菌对4种不同的抗菌药物(青霉素类、四环素类、硝基咪唑类和大环内酯类)耐药。这是第一份揭示多药耐药肺炎克雷伯菌在患病的加里宾鸡中发生的报告,这种情况对公共卫生构成风险。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Incidence and antimicrobial susceptibility profiles of multi-drug resistant Klebsiella pneumoniae strains isolated from diseased Clarias gariepinus.

Klebsiella pneumoniae is associated with substantial economic losses in the fish industry and with food-borne illness in humans. This study aimed to determine the incidence and antibiogram profiles of multi-drug resistant Klebsiella pneumoniae strains isolated from diseased Clarias gariepinus. A total of 384 diseased Clarias gariepinus that showed clinical signs of red spots and hemorrhages on the pectoral fin were sampled from commercial fish farms in Lagos, Ogun, and Oyo States, Nigeria. Samples of fin, liver, kidney, and gill were taken from diseased C. gariepinus. Samples were subjected to bacteriological examinations and isolates were identified based on morphological characteristics, biochemical tests, and 16 S rRNA gene characterisation. The retrieved isolates were tested for sequence analysis and antibiogram profile. The incidence of K. pneumoniae among the examined diseased Clarias gariepinus was 61.7% (237/384). The highest prevalence was observed in the gill (32.9%). The percentage of K. Pneumoniae isolates in diseased C. gariepinus from selected Southwest states were; Lagos State 46.8%, Ogun State 78.1%, and Oyo State 60.1% (p > 0.05). The phylogenetic analyses revealed that the tested K. pneumoniae strains shared high genetic similarity with other K. pneumoniae strains from South Africa, Saudi Arabia, India, USA, Spain, Bangladesh, and China. Antibiogram profiles revealed that 100% (120/120) of the retrieved K. pneumoniae strains were MDR to four different antimicrobial classes (penicillins, tetracyclines, nitroimidazole, and macrolides). This is the first report to reveal the occurrence of MDR K. pneumoniae in diseased C. gariepinus, an emergence that represents a risk to public health.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
Brazilian Journal of Microbiology
Brazilian Journal of Microbiology 生物-微生物学
CiteScore
4.10
自引率
4.50%
发文量
216
审稿时长
1.0 months
期刊介绍: The Brazilian Journal of Microbiology is an international peer reviewed journal that covers a wide-range of research on fundamental and applied aspects of microbiology. The journal considers for publication original research articles, short communications, reviews, and letters to the editor, that may be submitted to the following sections: Biotechnology and Industrial Microbiology, Food Microbiology, Bacterial and Fungal Pathogenesis, Clinical Microbiology, Environmental Microbiology, Veterinary Microbiology, Fungal and Bacterial Physiology, Bacterial, Fungal and Virus Molecular Biology, Education in Microbiology. For more details on each section, please check out the instructions for authors. The journal is the official publication of the Brazilian Society of Microbiology and currently publishes 4 issues per year.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:604180095
Book学术官方微信