{"title":"巴西Hope斑点气单胞菌和假单胞菌的抗性和适应性的基因组特征。","authors":"Anna Luiza Bauer Canellas, Marianne Pataro Mello, Rodolfo Paranhos, Marinella Silva Laport","doi":"10.1007/s42770-025-01706-w","DOIUrl":null,"url":null,"abstract":"<p><p>The Cagarras Islands Archipelago, a marine protected area in Rio de Janeiro, Brazil, is endowed with a rich and abundant biodiversity. Despite being a \"Hope Spot\" for marine conservation, it suffers from the impacts of urban pollution, given its proximity to a major metropolis. To date, few studies have delved into the characterization of the bacterial community in the Cagarras Islands, and limited knowledge regarding the pollution effects on a microbiological scale is available. This study presents the first genomic characterization of seven potentially pathogenic strains of Aeromonas and Pseudomonas isolated from water samples collected in four expeditions. Whole genome sequencing was conducted to assess antimicrobial and heavy metal resistance, as well as pathogenicity. Comparative genomic analyses were performed using additional Aeromonas and Pseudomonas genomes from different sources. The identification of multiple genes associated with heavy metal resistance, alongside clinically relevant antimicrobial resistance genes such as mcr-3 (conferring resistance to the last-resort antimicrobial colistin) and bla<sub>OXA</sub> (associated with beta-lactam resistance), showcases the adaptability of these strains and the presence of potentially pathogenic, and antibiotic-resistant bacteria in a protected marine ecosystem. This study highlights the complex genomic landscape of bacteria isolated from a Brazilian Hope Spot, underscoring the importance of integrated microbial monitoring strategies.</p>","PeriodicalId":9090,"journal":{"name":"Brazilian Journal of Microbiology","volume":" ","pages":"1641-1651"},"PeriodicalIF":1.9000,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12350978/pdf/","citationCount":"0","resultStr":"{\"title\":\"Genomic signatures of resistance and adaptability in Aeromonas and Pseudomonas from a Brazilian Hope Spot.\",\"authors\":\"Anna Luiza Bauer Canellas, Marianne Pataro Mello, Rodolfo Paranhos, Marinella Silva Laport\",\"doi\":\"10.1007/s42770-025-01706-w\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>The Cagarras Islands Archipelago, a marine protected area in Rio de Janeiro, Brazil, is endowed with a rich and abundant biodiversity. Despite being a \\\"Hope Spot\\\" for marine conservation, it suffers from the impacts of urban pollution, given its proximity to a major metropolis. To date, few studies have delved into the characterization of the bacterial community in the Cagarras Islands, and limited knowledge regarding the pollution effects on a microbiological scale is available. This study presents the first genomic characterization of seven potentially pathogenic strains of Aeromonas and Pseudomonas isolated from water samples collected in four expeditions. Whole genome sequencing was conducted to assess antimicrobial and heavy metal resistance, as well as pathogenicity. Comparative genomic analyses were performed using additional Aeromonas and Pseudomonas genomes from different sources. The identification of multiple genes associated with heavy metal resistance, alongside clinically relevant antimicrobial resistance genes such as mcr-3 (conferring resistance to the last-resort antimicrobial colistin) and bla<sub>OXA</sub> (associated with beta-lactam resistance), showcases the adaptability of these strains and the presence of potentially pathogenic, and antibiotic-resistant bacteria in a protected marine ecosystem. This study highlights the complex genomic landscape of bacteria isolated from a Brazilian Hope Spot, underscoring the importance of integrated microbial monitoring strategies.</p>\",\"PeriodicalId\":9090,\"journal\":{\"name\":\"Brazilian Journal of Microbiology\",\"volume\":\" \",\"pages\":\"1641-1651\"},\"PeriodicalIF\":1.9000,\"publicationDate\":\"2025-09-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12350978/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Brazilian Journal of Microbiology\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://doi.org/10.1007/s42770-025-01706-w\",\"RegionNum\":4,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2025/6/6 0:00:00\",\"PubModel\":\"Epub\",\"JCR\":\"Q3\",\"JCRName\":\"MICROBIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Brazilian Journal of Microbiology","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1007/s42770-025-01706-w","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/6/6 0:00:00","PubModel":"Epub","JCR":"Q3","JCRName":"MICROBIOLOGY","Score":null,"Total":0}
Genomic signatures of resistance and adaptability in Aeromonas and Pseudomonas from a Brazilian Hope Spot.
The Cagarras Islands Archipelago, a marine protected area in Rio de Janeiro, Brazil, is endowed with a rich and abundant biodiversity. Despite being a "Hope Spot" for marine conservation, it suffers from the impacts of urban pollution, given its proximity to a major metropolis. To date, few studies have delved into the characterization of the bacterial community in the Cagarras Islands, and limited knowledge regarding the pollution effects on a microbiological scale is available. This study presents the first genomic characterization of seven potentially pathogenic strains of Aeromonas and Pseudomonas isolated from water samples collected in four expeditions. Whole genome sequencing was conducted to assess antimicrobial and heavy metal resistance, as well as pathogenicity. Comparative genomic analyses were performed using additional Aeromonas and Pseudomonas genomes from different sources. The identification of multiple genes associated with heavy metal resistance, alongside clinically relevant antimicrobial resistance genes such as mcr-3 (conferring resistance to the last-resort antimicrobial colistin) and blaOXA (associated with beta-lactam resistance), showcases the adaptability of these strains and the presence of potentially pathogenic, and antibiotic-resistant bacteria in a protected marine ecosystem. This study highlights the complex genomic landscape of bacteria isolated from a Brazilian Hope Spot, underscoring the importance of integrated microbial monitoring strategies.
期刊介绍:
The Brazilian Journal of Microbiology is an international peer reviewed journal that covers a wide-range of research on fundamental and applied aspects of microbiology.
The journal considers for publication original research articles, short communications, reviews, and letters to the editor, that may be submitted to the following sections: Biotechnology and Industrial Microbiology, Food Microbiology, Bacterial and Fungal Pathogenesis, Clinical Microbiology, Environmental Microbiology, Veterinary Microbiology, Fungal and Bacterial Physiology, Bacterial, Fungal and Virus Molecular Biology, Education in Microbiology. For more details on each section, please check out the instructions for authors.
The journal is the official publication of the Brazilian Society of Microbiology and currently publishes 4 issues per year.