Lívia do Carmo Silva, Isabela Cristina de Oliveira Dos Santos, Juliana Santana de Curcio, Allana de Paula Castilho, João Vitor Gregolan Barbosa, Luiz Henrique Alves Costa, Álvaro Ferreira Júnior, Elisângela de Paula Silveira-Lacerda
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引用次数: 0
Abstract
Early diagnosis of sporotrichosis, along with the surveillance of fungal circulation in both human and domestic animal populations, is critical for global health. Although the gold standard method is microbiological culture followed by microscopic analysis of the fungus, this methodology requires a long period and skilled professionals for pathogen identification. We developed a molecular assay and conducted a preliminary evaluation using clinical samples using Loop-Mediated Isothermal Amplification (LAMP) technology that detects Sporothrix schenckii and Sporothrix brasiliensis fungi, with results in 30 min. Results are visually interpreted and do not require DNA extraction, making it a promising tool for point-of-care diagnosis in veterinary settings.
期刊介绍：
The Brazilian Journal of Microbiology is an international peer reviewed journal that covers a wide-range of research on fundamental and applied aspects of microbiology.
The journal considers for publication original research articles, short communications, reviews, and letters to the editor, that may be submitted to the following sections: Biotechnology and Industrial Microbiology, Food Microbiology, Bacterial and Fungal Pathogenesis, Clinical Microbiology, Environmental Microbiology, Veterinary Microbiology, Fungal and Bacterial Physiology, Bacterial, Fungal and Virus Molecular Biology, Education in Microbiology. For more details on each section, please check out the instructions for authors.
The journal is the official publication of the Brazilian Society of Microbiology and currently publishes 4 issues per year.
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