Biological synthesis of gold nanoparticles using endophytic fungus Cladosporium sphaerospermum (RUV07) extract: characterization and in vitro antiproliferative effect of A549 cells.

IF 1.9 4区 生物学 Q3 MICROBIOLOGY
Brazilian Journal of Microbiology Pub Date : 2025-09-01 Epub Date: 2025-04-26 DOI:10.1007/s42770-025-01675-0
Surendirakumar Kannaiah, Prabhu Raju, Narmatha Sekar, Bandana Sanasam, Thokchom Nepolian Singh, Nongthombam Kistu Singh, Sathish Muthukrishnan
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Abstract

In this groundbreaking research, we utilized the fungal extract of Cladosporium sphaerospermum -RVU07 as a powerful reducing agent to create bioactive gold nanoparticles (Au-NPs). The comprehensive material characterization of the Au-NPs was conducted using methods including XRD, SEM, TEM, UV-vis, and DLS analysis. Notably, the successful formation of Au-NPs was confirmed by a striking color change of the precursor from pale yellow to vibrant violet. This transformation underscores the pivotal role of fungal bioactive molecules, which impart distinctive physicochemical and biological properties to the Au-NPs. The extraction of these compounds significantly influenced both the particle size and surface morphology, enhancing their effectiveness. We investigated the antiproliferative effect of the bioactive Au-NPs against lung cancer cells specifically A549. Our findings revealed that these nanoparticles and bioactive molecules effectively induce ROS production and drive nuclear damage-mediated cell death. With their nanoscale dimensions, the Au-NPs rapidly penetrate the atomic region of A549 cancer cells, facilitating substantial cellular dysfunction. Our nuclear damage assays confirmed that the Au-NPs lead to pronounced nuclear fragmentation and shrinkage in A549 cells following treatment. Additionally, we evaluated the biocompatibility of the Au-NPs against PBMC cells. The biosafety assay results convincingly showed that the bioactive molecules on the Au-NPs' surface significantly mitigate their toxic effects on normal cells. This research highlights the immense potential of mycosynthesized bioactive Au-NPs as innovative pharmaceuticals for a wide array of cancer therapies and biomedical applications, heralding a new era in targeted treatment solutions.

内生真菌球藻枝孢(Cladosporium sphaerospermum, RUV07)提取物生物合成纳米金:表征及体外抗A549细胞增殖作用
在这项开创性的研究中,我们利用球形枝孢菌的真菌提取物-RVU07作为强效还原剂来制备生物活性金纳米粒子(Au-NPs)。采用XRD、SEM、TEM、UV-vis、DLS等方法对Au-NPs进行了全面的材料表征。值得注意的是,Au-NPs的成功形成是由前体从淡黄色到鲜艳的紫色的显著颜色变化所证实的。这种转变强调了真菌生物活性分子的关键作用,这些活性分子赋予Au-NPs独特的物理化学和生物特性。这些化合物的提取显著影响了颗粒大小和表面形貌,增强了它们的有效性。我们研究了生物活性Au-NPs对肺癌细胞A549的抗增殖作用。我们的研究结果表明,这些纳米颗粒和生物活性分子有效地诱导活性氧的产生,并驱动核损伤介导的细胞死亡。凭借其纳米级的尺寸,Au-NPs迅速穿透A549癌细胞的原子区域,促进实质性的细胞功能障碍。我们的核损伤试验证实,Au-NPs导致A549细胞在治疗后出现明显的核碎裂和收缩。此外,我们还评估了Au-NPs对PBMC细胞的生物相容性。生物安全性试验结果令人信服地表明,Au-NPs表面的生物活性分子显著减轻了它们对正常细胞的毒性作用。这项研究强调了真菌合成的生物活性Au-NPs作为广泛的癌症治疗和生物医学应用的创新药物的巨大潜力,预示着靶向治疗解决方案的新时代。
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来源期刊
Brazilian Journal of Microbiology
Brazilian Journal of Microbiology 生物-微生物学
CiteScore
4.10
自引率
4.50%
发文量
216
审稿时长
1.0 months
期刊介绍: The Brazilian Journal of Microbiology is an international peer reviewed journal that covers a wide-range of research on fundamental and applied aspects of microbiology. The journal considers for publication original research articles, short communications, reviews, and letters to the editor, that may be submitted to the following sections: Biotechnology and Industrial Microbiology, Food Microbiology, Bacterial and Fungal Pathogenesis, Clinical Microbiology, Environmental Microbiology, Veterinary Microbiology, Fungal and Bacterial Physiology, Bacterial, Fungal and Virus Molecular Biology, Education in Microbiology. For more details on each section, please check out the instructions for authors. The journal is the official publication of the Brazilian Society of Microbiology and currently publishes 4 issues per year.
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