Antimicrobial resistance and genetic diversity of Escherichia coli isolated from marine bivalves.

IF 1.9 4区 生物学 Q3 MICROBIOLOGY
Brazilian Journal of Microbiology Pub Date : 2025-09-01 Epub Date: 2025-05-22 DOI:10.1007/s42770-025-01696-9
Jailton Lobo da Costa Lima, Marcelly Miranda Aybal Jayme, Felipe Piedade Gonçalves Neves, Angela Corrêa de Freitas-Almeida, Mara Lúcia Penna Queiroz, Fabio Vieira de Araujo
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引用次数: 0

Abstract

Escherichia coli can contaminate the marine environment through sewage and accumulate in bivalve molluscs. We assessed antimicrobial resistance (AMR) mechanisms and genetic diversity of 69 E. coli isolates recovered from in natura, boiled, and purchased Perna perna mussels in Niterói city, Brazil. All isolates were sensitive to cefoxitin, imipenem, meropenem, and fosfomycin. In contrast, 61 (88.4%) isolates were not susceptible to streptomycin. We found ten (14.5%) multidrug-resistant (MDR) isolates. Extended-spectrum beta-lactamase (ESBL) production was detected in 30 (43.5%) isolates, with 14 (46.7%) isolates carrying ESBL genes (blaTEM, blaCTX-M9, blaSHV, blaCTX-M2, and blaCTX-M8/25). Quinolone/fluoroquinolone resistance was observed in 12 (17.4%) isolates, with qnrS and qnrB genes identified. Nine (13%) isolates were resistant to folate pathway inhibitors, with sul1 and sul2 genes identified. Only one (1.5%) isolate had the intI integrase gene. Most isolates had the A/C replicon (73.9%) and belonged to phylogenetic group B1 (50.7%). PFGE analysis revealed the existence of 54 genotypes, with four clusters containing isolates from different sources. The presence of ESBL-producing strains, AMR genes, and diverse plasmid replicons highlights the role of marine environments in AMR dissemination. Continuous AMR surveillance in marine ecosystems is crucial to mitigate public health risks linked to seafood consumption.

海洋双壳类分离的大肠埃希菌的耐药性和遗传多样性。
大肠杆菌可以通过污水污染海洋环境,并在双壳类软体动物体内积累。我们评估了从巴西Niterói市的天然、煮熟和购买的Perna Perna贻贝中分离的69株大肠杆菌的抗微生物药物耐药性机制和遗传多样性。所有分离株对头孢西丁、亚胺培南、美罗培南和磷霉素均敏感。61株(88.4%)对链霉素不敏感。我们发现10株(14.5%)耐多药(MDR)菌株。30株(43.5%)分离物检测到广谱β -内酰胺酶(ESBL)产生,14株(46.7%)分离物携带ESBL基因(blaTEM、blaCTX-M9、blaSHV、blaCTX-M2和blaCTX-M8/25)。12株(17.4%)对喹诺酮类/氟喹诺酮类药物耐药,鉴定出qnrS和qnrB基因。9株(13%)菌株对叶酸途径抑制剂具有耐药性,其中sul1和sul2基因已被鉴定。只有1株(1.5%)具有整合酶基因。大多数分离株存在A/C复制子(73.9%),属于系统发育类群B1(50.7%)。PFGE分析显示存在54个基因型,其中4个聚类包含来自不同来源的分离株。产生esbl的菌株、抗菌素耐药性基因和多种质粒复制子的存在突出了海洋环境在抗菌素耐药性传播中的作用。在海洋生态系统中持续监测抗微生物药物耐药性对于减轻与海产品消费有关的公共卫生风险至关重要。
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来源期刊
Brazilian Journal of Microbiology
Brazilian Journal of Microbiology 生物-微生物学
CiteScore
4.10
自引率
4.50%
发文量
216
审稿时长
1.0 months
期刊介绍: The Brazilian Journal of Microbiology is an international peer reviewed journal that covers a wide-range of research on fundamental and applied aspects of microbiology. The journal considers for publication original research articles, short communications, reviews, and letters to the editor, that may be submitted to the following sections: Biotechnology and Industrial Microbiology, Food Microbiology, Bacterial and Fungal Pathogenesis, Clinical Microbiology, Environmental Microbiology, Veterinary Microbiology, Fungal and Bacterial Physiology, Bacterial, Fungal and Virus Molecular Biology, Education in Microbiology. For more details on each section, please check out the instructions for authors. The journal is the official publication of the Brazilian Society of Microbiology and currently publishes 4 issues per year.
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