利用高分辨率熔融分析区分猫肠道和猫传染性腹膜炎冠状病毒。

IF 1.9 4区 生物学 Q3 MICROBIOLOGY
Brazilian Journal of Microbiology Pub Date : 2025-09-01 Epub Date: 2025-07-21 DOI:10.1007/s42770-025-01742-6
Wei Zhang, Jinglong Luo, Litao Yan, Li Xiao
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引用次数: 0

摘要

目的:猫冠状病毒(FCoV)感染在全球猫科动物种群中广泛发生。FCoV家族包括两种不同的生物型:猫肠道冠状病毒(FECV)和猫感染性腹膜炎病毒(FIPV)。FECV感染无症状或仅导致轻度肠道疾病,而FIPV可导致高死亡率。区分FIPV和FCoV对于及时治疗至关重要。高分辨率熔融(HRM)技术可以准确地解决这一问题。方法:从FCoV的7b基因设计一对引物。在FECV和FIPV之间有三种特定于相同片段扩增的突变。因此,这些突变导致不同的解离曲线和Tm值。结果:HRM分析显示,FIPV的Tm值比FECV高约2°C。本方法具有较高的特异性、敏感性和重复性。我们在11个临床样本中测试了该方法的高准确性,并通过DNA测序证实了这一点。高分辨率熔化曲线分析提供了区分FIPV和FCoV的另一种方法。结论及相关性:这种基于hrm的检测方法提供了快速(
本文章由计算机程序翻译,如有差异,请以英文原文为准。
The use of high-resolution melting analysis to distinguish feline enteric and feline infectious peritonitis coronaviruses.

Objectives: Feline coronavirus (FCoV) infections are a widespread occurrence among feline populations globally. The FCoV family encompasses two distinct biotypes: feline enteric coronavirus (FECV) and feline infectious peritonitis viruses (FIPV). FECV infections are asymptomatic or result in only mild enteric disease, whereas FIPV can cause a high mortality rate. Distinguishing FIPV from FCoV is crucial for prompt treatment. High resolution Melting (HRM) technology can accurately solve this problem.

Methods: In our work, one pair of primers was designed from the 7b gene of FCoV. There are three mutations specific for the same fragment amplification between FECV and FIPV. Thus, these mutations causes different dissociation curves and Tm values.

Results: HRM analysis showed that Tm values of FIPV was about 2 °C higher than that of FECV. Our assay had high specificity, sensitivity and reproducibility. We have tested the high accuracy of this method in 11 clinical samples and confirmed it with DNA sequencing. The high-resolution melting curve assay provides an alternative mean to distinguish FIPV from FCoV.

Conclusions and relevance: This HRM-based assay provides a rapid (< 2 h), cost-effective alternative to sequencing for discriminating FIPV from FECV. Its high accuracy and suitability for routine diagnostics could significantly improve early FIPV identification, enabling timely therapeutic decisions in veterinary practice.

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来源期刊
Brazilian Journal of Microbiology
Brazilian Journal of Microbiology 生物-微生物学
CiteScore
4.10
自引率
4.50%
发文量
216
审稿时长
1.0 months
期刊介绍: The Brazilian Journal of Microbiology is an international peer reviewed journal that covers a wide-range of research on fundamental and applied aspects of microbiology. The journal considers for publication original research articles, short communications, reviews, and letters to the editor, that may be submitted to the following sections: Biotechnology and Industrial Microbiology, Food Microbiology, Bacterial and Fungal Pathogenesis, Clinical Microbiology, Environmental Microbiology, Veterinary Microbiology, Fungal and Bacterial Physiology, Bacterial, Fungal and Virus Molecular Biology, Education in Microbiology. For more details on each section, please check out the instructions for authors. The journal is the official publication of the Brazilian Society of Microbiology and currently publishes 4 issues per year.
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