Brazilian Journal of Medical and Biological Research最新文献_第7页

Brief results of heterotopic xenotransplantation of meningioma into the peritoneum of Wistar rats: an experimental study. Wistar大鼠脑膜瘤异位异种移植腹腔的实验研究。

IF 1.9 4区医学

Brazilian Journal of Medical and Biological Research Pub Date : 2026-03-02 eCollection Date: 2026-01-01 DOI: 10.1590/1414-431X2025e15012

L F M da Silva Junior, M J S Dos Santos, A O do Carmo, R O L Silva, M A G Campos, G E B Silva, O J Dos Santos, N Salgado Filho

{"title":"Brief results of heterotopic xenotransplantation of meningioma into the peritoneum of Wistar rats: an experimental study.","authors":"L F M da Silva Junior, M J S Dos Santos, A O do Carmo, R O L Silva, M A G Campos, G E B Silva, O J Dos Santos, N Salgado Filho","doi":"10.1590/1414-431X2025e15012","DOIUrl":"10.1590/1414-431X2025e15012","url":null,"abstract":"Meningiomas are the most common primary intracranial tumors, and xenotransplantation models help to study their behavior and test therapies. This research developed a model implanting WHO grade I meningothelial meningioma into the peritoneum of immunosuppressed rats, comparing it to subcutaneous implantation. The primary objective was to analyze tumor growth and progression, focusing on the role of the microenvironment. Peritoneal implants (1.87±0.25 cm) grew significantly larger than subcutaneous implants (0.86±0.14 cm) (P<0.0001). An inverse correlation was found between weight variation and the difference in implant sizes, indicating that weight loss in animals was associated with larger implant growth. Animals that lost weight had significantly larger implants compared to those that gained weight. Implantation site and the animal's weight variation can significantly impact the growth of meningothelial meningioma fragments, with peritoneal implants showing greater growth and weight loss correlating with larger tumors.","PeriodicalId":9088,"journal":{"name":"Brazilian Journal of Medical and Biological Research","volume":"59 ","pages":"e15012"},"PeriodicalIF":1.9,"publicationDate":"2026-03-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12990303/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147353777","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Therapeutic potential of p-cymene in mitigating alcohol-induced damage in umbilical cord-derived mesenchymal stem cells through restoration of Nanog, VEGF, and antioxidants levels; in silico and in vitro approaches. 对伞花素通过恢复Nanog、VEGF和抗氧化剂水平减轻酒精诱导的脐带间充质干细胞损伤的治疗潜力在硅和体外的方法。

IF 1.9 4区医学

Brazilian Journal of Medical and Biological Research Pub Date : 2026-03-02 eCollection Date: 2026-01-01 DOI: 10.1590/1414-431X2025e15185

N Aziz, T Maqbool, M Arooj, H S Afzal, A Altaf, M Atif, S Naz, M N H Malik

{"title":"Therapeutic potential of p-cymene in mitigating alcohol-induced damage in umbilical cord-derived mesenchymal stem cells through restoration of Nanog, VEGF, and antioxidants levels; in silico and in vitro approaches.","authors":"N Aziz, T Maqbool, M Arooj, H S Afzal, A Altaf, M Atif, S Naz, M N H Malik","doi":"10.1590/1414-431X2025e15185","DOIUrl":"10.1590/1414-431X2025e15185","url":null,"abstract":"Human stem cells can divide and differentiate into various cell types. Umbilical cord-derived mesenchymal stem cells (UC-MSCs) are multipotent cells with high regenerative, anti-inflammatory, and immunomodulatory properties. Ethanol, the main component of alcoholic beverages, induces cytotoxicity and oxidative stress in cells. In recent years, natural compounds have gained attention for their potential protective effects against ethanol-induced cellular damage. p-Cymene is one such compound that acts as an antioxidant. This study aimed to evaluate the potential of p-cymene to reduce the harmful effects of alcohol on ethanol-induced cytotoxicity and oxidative stress in UC-MSCs through in silico and in vitro approaches. In silico pharmacokinetic and toxicity analyses of p-cymene were used, followed by in vitro evaluation using ethanol-injured UC-MSCs. Cell viability, antioxidant [glutathione (GSH), super-oxide dismutase (SOD)], inflammatory, proliferative, apoptotic, and wound healing assays were performed across different concentrations to assess protective effects. The pharmacokinetic analysis showed that p-cymene exhibited considerable pharmacokinetic properties by following Lipinski's Rule of Five. Toxicity analysis revealed no toxic effects of p-cymene, suggesting its potential as a natural compound. Further in vitro experimentation showed that p-cymene independently restored cell viability, reduced inflammation, stabilized Nanog, improved vascular endothelial growth factor (VEGF), enhanced antioxidants (GSH, SOD), promoted wound healing, and reduced cell death in ethanol-injured cells, with the 50 µM concentration being the most effective. These findings support our hypothesis that p-cymene protects UC-MSCs from ethanol-induced damage.","PeriodicalId":9088,"journal":{"name":"Brazilian Journal of Medical and Biological Research","volume":"59 ","pages":"e15185"},"PeriodicalIF":1.9,"publicationDate":"2026-03-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12990310/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147353791","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Precision application of target-controlled infusion of esketamine combined with sufentanil in anesthesia for thoracoscopic surgery and its effect on hemodynamics, postoperative pain, and safety. 靶控输注艾氯胺酮联合舒芬太尼在胸腔镜手术麻醉中的精确应用及其对血流动力学、术后疼痛和安全性的影响。

IF 1.9 4区医学

Brazilian Journal of Medical and Biological Research Pub Date : 2026-03-02 eCollection Date: 2026-01-01 DOI: 10.1590/1414-431X2025e15157

Tianyu Qi, Xiangliu Liu, Sen Liu, Anqi Yin, Lidong Zhang

{"title":"Precision application of target-controlled infusion of esketamine combined with sufentanil in anesthesia for thoracoscopic surgery and its effect on hemodynamics, postoperative pain, and safety.","authors":"Tianyu Qi, Xiangliu Liu, Sen Liu, Anqi Yin, Lidong Zhang","doi":"10.1590/1414-431X2025e15157","DOIUrl":"10.1590/1414-431X2025e15157","url":null,"abstract":"We aimed to expound the precise application of target-controlled infusion (TCI) of esketamine combined with sufentanil in anesthesia for video-assisted thoracoscopic surgery (VATS) and its effect on hemodynamics, postoperative pain, and safety. Eighty patients scheduled for thoracoscopic procedures were randomly assigned to either a control group [n=40, conventional empiric anesthesia (sufentanil plus propofol)] or an observation group [n=40, TCI of esketamine and sufentanil]. Hemodynamic indices [mean arterial pressure (MAP), heart rate (HR), central venous pressure (CVP), stroke volume (SV), cardiac output (CO), systemic vascular resistance (SVR), and oxygen saturation (SpO2)] were recorded before anesthesia (T0), after induction of anesthesia (T1), 30 min of anesthesia (T2), and at the end of surgery (T3). Recovery profiles [length of stay in the post-anesthesia care unit (PACU), awakening time], Ramsay sedation scores (T0-T3), visual analog scale (VAS) pain scores at 2, 24, and 48 h post-op, and adverse event rates were compared. The observation group showed smaller hemodynamic fluctuations from T1 to T3. At T3, this group had higher MAP, SV, and CO (P<0.05), steadier CVP and SVR, faster recovery (PACU stay and awakening times shorter, P<0.01), lower VAS scores at 24 and 48 h, higher Ramsay scores at T2 and T3, and lower overall adverse event rates (P=0.018) than the control group. TCI of esketamine plus sufentanil improved intraoperative hemodynamic stability, shortened recovery, enhanced early analgesia, and reduced adverse reactions in VATS, supporting its precision and safety.","PeriodicalId":9088,"journal":{"name":"Brazilian Journal of Medical and Biological Research","volume":"59 ","pages":"e15157"},"PeriodicalIF":1.9,"publicationDate":"2026-03-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12990302/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147353774","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

IF 1.9 4区医学

Brazilian Journal of Medical and Biological Research Pub Date : 2026-03-02 eCollection Date: 2026-01-01 DOI: 10.1590/1414-431X2025e15097

Zhixiang Mao, Linjian Zhou, Yi Nie, Hongbing Wang, Junying Zhang

{"title":"Drug-related proteinuria: a vigilance analysis based on the FAERS database.","authors":"Zhixiang Mao, Linjian Zhou, Yi Nie, Hongbing Wang, Junying Zhang","doi":"10.1590/1414-431X2025e15097","DOIUrl":"10.1590/1414-431X2025e15097","url":null,"abstract":"Proteinuria is a prevalent and significant adverse response (ADR) associated with numerous pharmaceuticals, and we employed the online public FDA Adverse Event Reporting System (FAERS) database to investigate a cohort of medications that may induce this ADR. This analysis aimed to identify and assess the most prevalent and significant medicines linked to the risk of proteinuria. We examined the publicly accessible FAERS database from 2004 to 2024. Utilizing the search term \"proteinuria\" and classifying by generic drug name, we aggregated reports of drug-related responses or trends in proteinuria, subsequently analyzing the data through a combination of ratio-of-reported-ratio (ROR) and proportional-reported-ratio (PRR) to identify and examine twelve medications that may induce proteinuria. A total of 16,355 adverse event reports related to proteinuria were identified in the FAERS database between 2004 and 2024. Among these, 21 drugs demonstrated statistically significant associations with proteinuria based on multivariate logistic regression, with the highest signals observed for voclosporin (ROR: 63.57) and lenvatinib (ROR: 41.01). Drug classes most strongly associated included anticancer agents, immunosuppressants, and antiviral drugs. Notably, the onset of proteinuria varied significantly across drug types, with anti-inflammatory agents showing the earliest median onset (5.4 days), while digestive system drugs and antivirals exhibited delayed onset exceeding 1,000 days on average. These findings underscore the need for early and long-term renal monitoring depending on drug category. Prompt assessment of nephrotoxicity risk is essential during the initial phase of medication, hence offering a more precise foundation for drug screening and optimization.","PeriodicalId":9088,"journal":{"name":"Brazilian Journal of Medical and Biological Research","volume":"59 ","pages":"e15097"},"PeriodicalIF":1.9,"publicationDate":"2026-03-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12990312/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147353749","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Perinatal and laboratory determinants of fortified human milk use: a case-control study. 强化母乳使用的围产期和实验室决定因素：一项病例对照研究。

IF 1.9 4区医学

Brazilian Journal of Medical and Biological Research Pub Date : 2026-02-16 eCollection Date: 2026-01-01 DOI: 10.1590/1414-431X2025e14750

C B G Tavares, J M V Zacarias, V H Souza, J V Visentainer, J E L Visentainer

{"title":"Perinatal and laboratory determinants of fortified human milk use: a case-control study.","authors":"C B G Tavares, J M V Zacarias, V H Souza, J V Visentainer, J E L Visentainer","doi":"10.1590/1414-431X2025e14750","DOIUrl":"10.1590/1414-431X2025e14750","url":null,"abstract":"This case-control study compared perinatal and laboratory factors between preterm infants who received unfortified human milk (HM) and those who received HM fortified with FM85®. The sample included 38 low birth weight (LBW) preterm infants admitted to a neonatal intensive care unit between June 2022 and May 2023, excluding those with comorbidities or formula use. Perinatal variables including gestational age, birth weight, head circumference (HC), and mode of delivery were analyzed for associations with fortifier use using chi-squared and Student's t-tests. Laboratory values were compared across nutritional stages using the Kruskal-Wallis and Mann-Whitney tests. Very preterm infants (confidence interval [CI]: 4.02-21.10; P<0.001) and those with LBW (CI: 1.30-5.75; P=0.009) were more likely to receive the fortifier, particularly those with poor weight progression during trophic feeding (CI: 1.12-16.30; P=0.039). Altered HC (CI: 1.49-8.24; P=0.004) and cesarean delivery (CI: 2.24-11.30; P<0.001) were also associated with FM85® use. Both groups showed laboratory abnormalities with neutrophils, initially elevated, decreasing (P<0.0001), while lymphocytes increased (P<0.0001). Red blood cell (P=0.00026) and hemoglobin levels (P=0.00007) worsened. Sodium and calcium levels rose significantly (P<0.0001 for both). HM fortification did not significantly alter most laboratory values, except for red blood cell count. LBW, altered HC, and cesarean delivery were associated with a greater likelihood of fortifier use. Immunological changes occurred even with fortification. Homologous fortifiers may represent a more physiological alternative.","PeriodicalId":9088,"journal":{"name":"Brazilian Journal of Medical and Biological Research","volume":"59 ","pages":"e14750"},"PeriodicalIF":1.9,"publicationDate":"2026-02-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12919753/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146225534","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sorting nexin 10 knockdown: new strategies for alleviating sepsis-associated acute lung injury. 筛选连接蛋白10敲低：减轻脓毒症相关急性肺损伤的新策略。

IF 1.9 4区医学

Brazilian Journal of Medical and Biological Research Pub Date : 2026-02-16 eCollection Date: 2026-01-01 DOI: 10.1590/1414-431X2025e15117

Min Wei, Jian Yang, Zejia Yu, Shan Li, Hui Xie, Shiyang Yuan, Qiujie Huang, Hui Zhang, Jun Feng

{"title":"Sorting nexin 10 knockdown: new strategies for alleviating sepsis-associated acute lung injury.","authors":"Min Wei, Jian Yang, Zejia Yu, Shan Li, Hui Xie, Shiyang Yuan, Qiujie Huang, Hui Zhang, Jun Feng","doi":"10.1590/1414-431X2025e15117","DOIUrl":"10.1590/1414-431X2025e15117","url":null,"abstract":"Acute lung injury (ALI) is a common complication of sepsis in connection with excessive inflammation and accumulation of oxidative stress. Sorting nexin 10 (SNX10) is a sorting nexin family member involved in inflammatory processes. This study aimed to explore the function of SNX10 in ALI. The cecal ligation and puncture (CLP) model was established to induce ALI in C57BL/6J mice. CLP mice exhibited elevated levels of SNX10 expression in the lung tissues. Mice were intratracheally injected with 50 μL adenovirus (108 PFU) containing short hairpin RNA plasmid targeting SNX10. SNX10 knockdown mice showed remission of CLP-induced pulmonary edema, hemorrhage, inflammatory infiltration, and thickened alveolar septum. SNX10 downregulation reduced reactive oxygen species (ROS) levels, increased superoxide dismutase activity and glutathione content, and decreased malondialdehyde content in the lung tissues. SNX10 knockdown decreased the phosphorylation of NF-κB p65 and its nuclear translocation, thus inhibiting the levels of tumor necrosis factor (TNF)-α and interleukin (IL)-6. Furthermore, SNX10 downregulation inhibited the NLRP3, p20 caspase 1, and ASC protein levels and the levels of IL-18 and IL-1β. A549 cells were treated with lipopolysaccharide (LPS) (10 μg/mL) for 24 h to simulate the inflammatory condition and SNX10 was knocked down using small interfering RNA. SNX10 knockdown cells showed increased viability and less ROS accumulation. Consistent with the in vivo results, the NF-κB/NLRP3 pathway and the secretion of inflammatory cytokines were inhibited after SNX10 knockdown in A549 cells. In summary, SNX10 downregulation mitigated sepsis-induced oxidative stress and pulmonary inflammation by inhibiting the NF-κB/NLRP3 pathway.","PeriodicalId":9088,"journal":{"name":"Brazilian Journal of Medical and Biological Research","volume":"59 ","pages":"e15117"},"PeriodicalIF":1.9,"publicationDate":"2026-02-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12919756/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146225526","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Energy production pathways of female soccer players during championships: a metabolomics approach. 女子足球运动员在锦标赛期间的能量产生途径：代谢组学方法。

IF 1.9 4区医学

Brazilian Journal of Medical and Biological Research Pub Date : 2026-02-16 eCollection Date: 2026-01-01 DOI: 10.1590/1414-431X2025e14589

M B A Nascimento, M M S Gouveia, M P P Santos, E R da Rocha-Junior, A C Crispim, E S Bento, T M Aquino, F A B Sousa, G G de Araujo, T Ataide-Silva

{"title":"Energy production pathways of female soccer players during championships: a metabolomics approach.","authors":"M B A Nascimento, M M S Gouveia, M P P Santos, E R da Rocha-Junior, A C Crispim, E S Bento, T M Aquino, F A B Sousa, G G de Araujo, T Ataide-Silva","doi":"10.1590/1414-431X2025e14589","DOIUrl":"10.1590/1414-431X2025e14589","url":null,"abstract":"Athletes mobilize both aerobic and anaerobic systems to produce energy during soccer matches, and it drastically modifies the concentration of metabolites related to muscle damage and energy metabolism. The fatigue-associated mechanism seems to encompass tricarboxylic acid cycle (TCA) disturbances, with a greater contribution of lipid-pathway metabolites in women during soccer matches. However, to the best of our knowledge, the effects of matches played during two championships on the metabolites and pathways associated with energy production in female players have not yet been described. Metabolomic analysis in sports context can better characterize the main metabolites related to energy production and the metabolic pathways. The aim of this study was to describe the variation in metabolites over the course of two championships in female players' urine, highlighting the occurrence of energy production-associated metabolites and the metabolic pathways they may arise from. Urine samples were collected before and after six matches of two championships. Nuclear magnetic resonance metabolomic approach was used for this purpose. Citrate, succinate, 1-methylnicotinamide, alpha-hydroxyisobutyrate, malonic and glycolic acids, phosphocreatine, and lactate were the compounds originated from energy generation processes with high scores in variable importance prediction (VIP), impacting on partial least squares discriminant analysis (PLS-DA) groupings of players. Phenylalanine biosynthesis, tyrosine and tryptophan biosynthesis, and the TCA cycle were mobilized before the matches, and the metabolism of taurine and hypotaurine were modulated across both moments.","PeriodicalId":9088,"journal":{"name":"Brazilian Journal of Medical and Biological Research","volume":"59 ","pages":"e14589"},"PeriodicalIF":1.9,"publicationDate":"2026-02-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12919752/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146225612","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cardiovascular system characteristics in HIV/AIDS patients with talaromycosis. HIV/AIDS患者伴talaromyosis的心血管系统特征

IF 1.9 4区医学

Brazilian Journal of Medical and Biological Research Pub Date : 2026-02-16 eCollection Date: 2026-01-01 DOI: 10.1590/1414-431X2025e14655

Lanwei Nong, Jie Ma, Yanqing Zheng, Qingdong Zhu, Cailing Wei, Jieling Chen, Sijun Li

{"title":"Cardiovascular system characteristics in HIV/AIDS patients with talaromycosis.","authors":"Lanwei Nong, Jie Ma, Yanqing Zheng, Qingdong Zhu, Cailing Wei, Jieling Chen, Sijun Li","doi":"10.1590/1414-431X2025e14655","DOIUrl":"10.1590/1414-431X2025e14655","url":null,"abstract":"Patients with acquired immune deficiency syndrome (AIDS) are more vulnerable to opportunistic infections (OIs) such as Talaromycosis marneffei (TSM), which is associated with a high mortality rate. Nevertheless, the effect of TSM on the cardiovascular system of affected patients remains elusive. To that end, this research aimed to investigate the impact of TSM on the cardiovascular system in the individuals with HIV/AIDS. Participants were assigned to the HIV/AIDS patients group or the HIV/AIDS patients with TSM (HIV/AIDS+TSM) group. A total of 120 individuals were included in the present study, with 59 in the HIV/AIDS group and 61 in the HIV/AIDS+TSM group. Myocardial serum markers, color Doppler cardiovascular ultrasound (CCU), and 24-h ambulatory electrocardiograph (AECG) data were collected and analyzed for both groups. Compared with the HIV/AIDS group, the HIV/AIDS+TSM group exhibited a significant increase in left and right diameters of the right atrium (LR-RA) and right ventricle (LR-RV), while the anteroposterior diameter of the right ventricle outflow tract (AT-RVOT), the interventricular septal thickness (IVS), and the left ventricular posterior wall thickness (LVPW) were significantly reduced. The HIV/AIDS+TSM group showed higher AECG abnormality rates, particularly for non-sinus rhythms and ST-T changes. Our findings demonstrated significant cardiac functional alterations in HIV/AIDS patients with TSM co-infection compared to HIV/AIDS alone, underscoring the necessity for enhanced cardiovascular monitoring in this vulnerable population.","PeriodicalId":9088,"journal":{"name":"Brazilian Journal of Medical and Biological Research","volume":"59 ","pages":"e14655"},"PeriodicalIF":1.9,"publicationDate":"2026-02-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12919754/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146225558","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Psychometric properties of the Pittsburgh Fatigability Scale for assessing physical and mental fatigability in Brazilian older adults (PFS-Brasil). 评估巴西老年人身心疲劳的匹兹堡疲劳量表（PFS-Brasil）的心理测量特性。

IF 1.9 4区医学

Brazilian Journal of Medical and Biological Research Pub Date : 2026-02-16 eCollection Date: 2026-01-01 DOI: 10.1590/1414-431X2025e14619

M S B Santos, J Bento-Torres, L L Santana, A A da Costa, I P P C Gesta, L M Silva, C W Picanço-Diniz, N W Glynn, N V O Bento-Torres

{"title":"Psychometric properties of the Pittsburgh Fatigability Scale for assessing physical and mental fatigability in Brazilian older adults (PFS-Brasil).","authors":"M S B Santos, J Bento-Torres, L L Santana, A A da Costa, I P P C Gesta, L M Silva, C W Picanço-Diniz, N W Glynn, N V O Bento-Torres","doi":"10.1590/1414-431X2025e14619","DOIUrl":"10.1590/1414-431X2025e14619","url":null,"abstract":"Fatigability is critical for understanding older adults' physical and mental health. The Pittsburgh Fatigability Scale (PFS) is widely employed to measure perceived fatigability, reflecting how fatigue impacts performance and its association with adverse health outcomes. This study aimed to evaluate the psychometric properties of the PFS adapted for Brazilian older adults (PFS-Brasil), focusing on perceived physical and mental fatigability. Confirmatory factor analysis (CFA) validated the bifactorial model. Internal consistency was assessed using Cronbach's alpha, while test-retest reliability was evaluated through the intraclass correlation coefficient (ICC) and Bland-Altman Plot. Convergent validity was determined by correlating PFS-Brasil score with physical and cognitive performance measures, and ceiling and floor effects were analyzed. CFA confirmed the two-factor structure of the PFS-Brasil. The Physical and Mental subscales showed high internal consistency (α=0.89 and 0.86, respectively). Test-retest reliability demonstrated good agreement (ICC for Physical=0.84; Mental=0.83). Higher fatigability score correlated with poorer physical performance on the Short Physical Performance Battery (SPPB) and the 6-Minute Walk Test (6MWT), with physical score showing weak to moderate correlations (P=-0.22 to -0.37) and mental score showing weak correlations (P=-0.20 to -0.25). Mental fatigability was weakly correlated with inhibitory control. The PFS-Brasil demonstrated robust psychometric properties, supporting its reliability and validity for assessing perceived physical and mental fatigue in older adults. Its use is recommended in clinical and research settings to identify individuals at risk of physical and psychological decline, promoting better health outcomes and quality of life in aging.","PeriodicalId":9088,"journal":{"name":"Brazilian Journal of Medical and Biological Research","volume":"59 ","pages":"e14619"},"PeriodicalIF":1.9,"publicationDate":"2026-02-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12919755/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146225568","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Expression of MIR155HG, LOC283856, KIAA0125, and LOC100190986 as potential prognostic and predictive biomarkers for breast cancer. MIR155HG、LOC283856、KIAA0125和LOC100190986作为乳腺癌潜在预后和预测性生物标志物的表达

IF 1.9 4区医学

Brazilian Journal of Medical and Biological Research Pub Date : 2026-02-06 eCollection Date: 2026-01-01 DOI: 10.1590/1414-431X2025e14805

A C Pavanelli, F R R Mangone, G P de Jesus, M A Nagai

{"title":"Expression of MIR155HG, LOC283856, KIAA0125, and LOC100190986 as potential prognostic and predictive biomarkers for breast cancer.","authors":"A C Pavanelli, F R R Mangone, G P de Jesus, M A Nagai","doi":"10.1590/1414-431X2025e14805","DOIUrl":"10.1590/1414-431X2025e14805","url":null,"abstract":"Accumulating evidence has pointed out that the altered expression of long non-coding RNAs (lncRNAs) is involved in the physiopathology of breast cancer (BC). However, the role of lncRNAs in BC progression remains poorly understood. Here, we evaluated cDNA microarray data from a previous study from our group to investigate the effects of SPARC expression on the transcriptome of MCF7 cells before and after treatment with docetaxel. We analyzed our gene expression data to identify differentially expressed long non-coding RNAs (DELncRNAs). In combination with in silico analysis, we selected a group of DELncRNAs with potential prognostic and predictive value for BC patients with tumors of different intrinsic subtypes. Overall, we identified 260 DELncRNAs comparing MCF7 cells with different expressions of SPARC after docetaxel treatment. Nine DELncRNAs (LOC646762, FLJ13224, CASC2, LOC100130691, MGC12916, LOC100190986, LOC283856, KIAA0125, and MIR155HG) showed significant associations with BC survival on the KM Plotter platform. Of these 9 DELncRNAs, MIR155HG, LOC283856, LOC100190986, and KIAA0125 were significantly correlated with recurrence-free survival and overall survival rates of BC patients, suggesting they could help predict the outcome of BC patients as prognostic factors. Moreover, in silico analysis showed that these DELncRNAs were able to predict BC patients' responses to different treatment protocols.","PeriodicalId":9088,"journal":{"name":"Brazilian Journal of Medical and Biological Research","volume":"59 ","pages":"e14805"},"PeriodicalIF":1.9,"publicationDate":"2026-02-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12892257/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146155968","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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