Gastrodin plays a protective role in alleviating hepatic ischemia reperfusion injury by regulating heme oxygenase-1 expression.

IF 1.9 4区 医学 Q2 BIOLOGY
Shan-Shan He, Han-Fei Huang, Shao-Qing Shi, Jing-Jiao Zhao, Bo Yuan, Xiang Ji, Hong-Bin Zhang
{"title":"Gastrodin plays a protective role in alleviating hepatic ischemia reperfusion injury by regulating heme oxygenase-1 expression.","authors":"Shan-Shan He, Han-Fei Huang, Shao-Qing Shi, Jing-Jiao Zhao, Bo Yuan, Xiang Ji, Hong-Bin Zhang","doi":"10.1590/1414-431X2024e14248","DOIUrl":null,"url":null,"abstract":"<p><p>Hepatic ischemia reperfusion injury (HIRI) is a pathophysiological and complex systemic process involving multiple tissues and organs. Gastrodin (GSTD), a natural compound from Gastrodia elata, displays a variety of interesting pharmacological activities. Heme oxygenase-1 (HO-1), a stress-responsive protein, has a cytoprotective defense response against oxidative and inflammatory injuries. The aim of this investigation was to elucidate whether GSTD plays a protective role against HIRI by regulating HO-1 expression. GSTD (100 mg/kg) or zinc protoporphyrin (15 mg/kg; an HO-1 inhibitor) was administered to HIRI C57 male mice. GSTD decreased glutamic pyruvic transaminase and glutamic oxaloacetic transaminase levels in HIRI mice. Inflammatory (TNF-α and IL-6) and oxidative-stress (malondialdehyde, MDA) markers of HIRI mice were decreased by GSTD. GSTD up-regulated HO-1 protein and mRNA expression in HIRI mice but decreased caspase-3 and -9 protein expression. GSTD lowered mRNA expression of apoptosis-related genes (caspase-3, -9, -12, and Bax) in the liver of HIRI mice but enhanced mRNA level of the anti-apoptotic Bcl-2 gene. Consistent with in vivo results, GSTD displayed a similar regulatory effect on the expression of mRNA (HO-1, caspase-3, -9, -12, Bax, and Bcl-2) and protein (HO-1, caspase-3 and -9) as well as inflammatory (TNF-α and IL-6) and on oxidative stress factors (superoxide dismutase and MDA) in BRL-3A cells transfected with small interfering HO-1 RNA in a hypoxia-reperfusion model. In conclusion, GSTD up-regulated HO-1 expression to play a protective role in HIRI by anti-apoptotic, anti-inflammatory, and antioxidant effects. GSTD is a promising natural compound that alleviated HIRI in liver surgery.</p>","PeriodicalId":9088,"journal":{"name":"Brazilian Journal of Medical and Biological Research","volume":"58 ","pages":"e14248"},"PeriodicalIF":1.9000,"publicationDate":"2025-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11793152/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Brazilian Journal of Medical and Biological Research","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1590/1414-431X2024e14248","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/1/1 0:00:00","PubModel":"eCollection","JCR":"Q2","JCRName":"BIOLOGY","Score":null,"Total":0}
引用次数: 0

Abstract

Hepatic ischemia reperfusion injury (HIRI) is a pathophysiological and complex systemic process involving multiple tissues and organs. Gastrodin (GSTD), a natural compound from Gastrodia elata, displays a variety of interesting pharmacological activities. Heme oxygenase-1 (HO-1), a stress-responsive protein, has a cytoprotective defense response against oxidative and inflammatory injuries. The aim of this investigation was to elucidate whether GSTD plays a protective role against HIRI by regulating HO-1 expression. GSTD (100 mg/kg) or zinc protoporphyrin (15 mg/kg; an HO-1 inhibitor) was administered to HIRI C57 male mice. GSTD decreased glutamic pyruvic transaminase and glutamic oxaloacetic transaminase levels in HIRI mice. Inflammatory (TNF-α and IL-6) and oxidative-stress (malondialdehyde, MDA) markers of HIRI mice were decreased by GSTD. GSTD up-regulated HO-1 protein and mRNA expression in HIRI mice but decreased caspase-3 and -9 protein expression. GSTD lowered mRNA expression of apoptosis-related genes (caspase-3, -9, -12, and Bax) in the liver of HIRI mice but enhanced mRNA level of the anti-apoptotic Bcl-2 gene. Consistent with in vivo results, GSTD displayed a similar regulatory effect on the expression of mRNA (HO-1, caspase-3, -9, -12, Bax, and Bcl-2) and protein (HO-1, caspase-3 and -9) as well as inflammatory (TNF-α and IL-6) and on oxidative stress factors (superoxide dismutase and MDA) in BRL-3A cells transfected with small interfering HO-1 RNA in a hypoxia-reperfusion model. In conclusion, GSTD up-regulated HO-1 expression to play a protective role in HIRI by anti-apoptotic, anti-inflammatory, and antioxidant effects. GSTD is a promising natural compound that alleviated HIRI in liver surgery.

Abstract Image

Abstract Image

Abstract Image

天麻素通过调节血红素氧化酶-1的表达,起到减轻肝脏缺血再灌注损伤的保护作用。
肝缺血再灌注损伤(HIRI)是一个涉及多组织、多器官的病理生理、复杂的全身性过程。天麻素是天麻中的一种天然化合物,具有多种药理活性。血红素加氧酶-1 (HO-1)是一种应激反应蛋白,对氧化和炎症损伤具有细胞保护性防御反应。本研究的目的是阐明GSTD是否通过调节HO-1表达对HIRI起保护作用。GSTD(100毫克/公斤)或原卟啉锌(15毫克/公斤);一种HO-1抑制剂)给予HIRI C57雄性小鼠。GSTD降低了HIRI小鼠的谷丙转氨酶和谷草酰乙酸转氨酶水平。GSTD降低了HIRI小鼠的炎症(TNF-α和IL-6)和氧化应激(丙二醛,MDA)标志物。GSTD上调HIRI小鼠HO-1蛋白和mRNA的表达,降低caspase-3和-9蛋白的表达。GSTD降低了HIRI小鼠肝脏中凋亡相关基因(caspase-3、-9、-12和Bax)的mRNA表达,但提高了抗凋亡基因Bcl-2的mRNA表达水平。与体内实验结果一致,GSTD对缺氧-再灌注模型中转染小干扰HO-1 RNA的BRL-3A细胞中mRNA (HO-1、caspase-3、-9、-12、Bax和Bcl-2)和蛋白(HO-1、caspase-3和-9)的表达以及炎症(TNF-α和IL-6)和氧化应激因子(超氧化物歧化酶和MDA)的表达具有类似的调节作用。综上所述,GSTD上调HO-1表达,通过抗凋亡、抗炎和抗氧化作用对HIRI起保护作用。GSTD是一种很有前途的天然化合物,可以减轻肝脏手术中的HIRI。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
CiteScore
4.00
自引率
0.00%
发文量
129
审稿时长
2 months
期刊介绍: The Brazilian Journal of Medical and Biological Research, founded by Michel Jamra, is edited and published monthly by the Associação Brasileira de Divulgação Científica (ABDC), a federation of Brazilian scientific societies: - Sociedade Brasileira de Biofísica (SBBf) - Sociedade Brasileira de Farmacologia e Terapêutica Experimental (SBFTE) - Sociedade Brasileira de Fisiologia (SBFis) - Sociedade Brasileira de Imunologia (SBI) - Sociedade Brasileira de Investigação Clínica (SBIC) - Sociedade Brasileira de Neurociências e Comportamento (SBNeC).
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:604180095
Book学术官方微信