BMC Veterinary Research最新文献

{"title":"A field comparison study of two vaccine protocols against Erysipelothrix rhusiopathiae in two types of swine breeds in Spain.","authors":"E Sanchez-Tarifa, C Alonso, I Perez, L A García, A Fernández-Fontelo, O Gómez-Duran, B García-Morante, Francisco A García-Vázquez, I Hernández-Caravaca","doi":"10.1186/s12917-024-04065-0","DOIUrl":"10.1186/s12917-024-04065-0","url":null,"abstract":"<p><p>Erysipelas still causes large economic losses to pig industry. Maternal immunity is critical to prevent erysipelas in young animals, thus, intensive vaccination protocols or practices focused on the improvement of the maternally derived immunity could provide substantial benefits. The present study evaluates potential changes in antibodies levels in sows and their offspring using two types of tests (commercial ELISA, Ingenasa or rSpaA415 ELISA) when two different vaccination programs (before farrowing or after farrowing) against Erysipelothrix rhusiopathiae were applied to sows from Iberian (A) or conventional Large White-Landrace (B) pig farms. The results showed a statistical correlation between titers found in sows and their one-week old piglets in both tests. The overall mean of (log) antibody titers in farm B measured by the commercial ELISA test was significantly higher in pre-farrowing vaccinated sows compared to the post-farrowing vaccine protocol (p = 0.0278). Additionally, using the rSpaA415 ELISA test, the overall mean of (log) antibody titers was significantly higher in pre-farrowing sows (p = 0.0056) compared to sows following post-farrowing vaccine protocol (p = 0.0003) or non- vaccinated sows. None of the above-mentioned differences were found in farm A. The overall mean of (log) antibody titers in piglets from the pre-farrowing vaccination protocol was significantly higher than piglets from the post-farrowing vaccination protocol in farm A (p = 0.0059; rSpaA415 ELISA) and farm B (p = 0.0168 and p = 0.0098 for the commercial and rSpaA415 ELISA data, respectively). Additionally, higher proportion of piglets from pre-farrowing vaccinated sows remained seropositive during the post-weaning period (days 42 to 84) compared to piglets from non-vaccinated or post-farrowing vaccinated groups in both farms A and B.</p>","PeriodicalId":9041,"journal":{"name":"BMC Veterinary Research","volume":"20 1","pages":"461"},"PeriodicalIF":2.3,"publicationDate":"2024-10-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11468219/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142406029","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction: Nano-selenium supplementation: improving growth, digestibility and mineral absorption in freshwater fish, Catla catla.","authors":"Nisar Ahmad, Syed Makhdoom Hussain, Shafaqat Ali, Muhammad Farrukh Tahir, Pallab K Sarker, Mudassar Shahid","doi":"10.1186/s12917-024-04318-y","DOIUrl":"10.1186/s12917-024-04318-y","url":null,"abstract":"","PeriodicalId":9041,"journal":{"name":"BMC Veterinary Research","volume":"20 1","pages":"465"},"PeriodicalIF":2.3,"publicationDate":"2024-10-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11468431/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142406030","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nucleotide sequence variants, gene expression and serum profile of immune and antioxidant markers associated with bacterial diarrhea susceptibility in Barki lambs.","authors":"Asmaa Darwish, Eman Ebissy, Amani Hafez, Ahmed Ateya, Ahmed El-Sayed","doi":"10.1186/s12917-024-04288-1","DOIUrl":"10.1186/s12917-024-04288-1","url":null,"abstract":"&lt;p&gt;&lt;strong&gt;Background: &lt;/strong&gt;Despite the fact that diarrhea is more accurately described as a clinical symptom than a disease. Diarrhea is one of the most important issues in ovine medicine, particularly in lambs, and because of high morbidity and mortality rate, sluggish growth performance, and veterinary costs, it is believed to be a major source of economic loss. Salmonella and enterotoxigenic Escherichia coli are the most common and commercially significant agents responsible for diarrhea.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Objective: &lt;/strong&gt;The objective of this study was to monitor the nucleotide sequence variations, gene expression, serum inflammatory and oxidative stress biomarkers in diarrheic lambs. Another aim was to identify different pathotypes and virulence genes of Salmonella and E. coli causing diarrhea.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Methodology: &lt;/strong&gt;Blood samples were taken from 50 Barki who were diarrheal and 50 who appeared to be healthy, and then divided in 3 portions, with EDTA added to the first part for CBC, DNA and RNA extraction. The second sample received 5000 I.U. of heparin calcium, and a clean plain tube was used for the third component. The second and third sections were centrifuged to extract serum and plasma until the biochemical and immunological analysis was completed. Fecal samples were collected for bacteriological examination, and the bacteria were identified by PCR analysis. PCR-DNA sequencing was conducted for immune (SELL, JAK2, SLC11A1, IL10, FEZF1, NCF4, LITAF, SBD2, NFKB, TNF-α, IL1B, IL6, LGALS, and CATH1), antioxidant (SOD1, CAT, GPX1, GST, Nrf2, Keap1, HMOX1, and NQO1), and GIT health (CALB1, GT, and MUC2) genes in healthy and diarrheic lambs.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Results: &lt;/strong&gt;Virulent genetic markers of pathogenic characteristics of E. coli (astA, Vt2e (Stx2e), CFA/I, groES and luxS) and Salmonella (invA, SopB, bcfC and avrA) were detected in all diarrheic lambs. PCR-DNA sequencing of immune, antioxidant and intestinal health genes found eleven single nucleotide polymorphisms (SNPs) linked to either diarrhea resistance or susceptibility in Barki lambs. Transcript levels of immune, antioxidant, and GIT health (CALB1, GT, and MUC2) genes varied between healthy and diarrheic lambs. Nucleotide sequence variation of the genes under inquiry between reference sequences in GenBank and those of the animals under investigation verified all identified SNPs. Significant (P = 0.001) erythrocytosis, neutrophilic leukocytosis, with lymphocytopenia were observed in diarrheic lambs. Significant (P = 0.001) increases in serum IL-1α, IL-1β, IL-6, TNF-α (90.5 ± 1.7, 101.8 ± 1.7, 72.3 ± 6.6, 71.26 ± 4.89 Pg/ml, respectively), serum Fb, Cp, Hp, SAA (230.7 ± 12.4 mg/dl, 6.5 ± 0.07 mg/dl, 2.5 ± 0.09 g/dl, 7.4 ± 0.4 mg/L, respectively), free radicals (MDA, NO), cortisol (6.91 ± 0.18 μg/dl) and growth hormone, with significant (P = 0.001) decreases in serum IL-10 (81.71 ± 1.05 Pg/ml), antioxidants (CAT, GPx), insulin, triiodothyronine (T3) and thyroxine ","PeriodicalId":9041,"journal":{"name":"BMC Veterinary Research","volume":"20 1","pages":"462"},"PeriodicalIF":2.3,"publicationDate":"2024-10-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11468138/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142406057","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Morphological identification and distribution comparison of telocytes in pituitary gland between normal and cryptorchid yaks.","authors":"Yumei Qi, Ligang Yuan, Jianlin Zeng, Xiaofen Wang, Long Ma, Jinghan Lv","doi":"10.1186/s12917-024-04307-1","DOIUrl":"10.1186/s12917-024-04307-1","url":null,"abstract":"<p><strong>Background: </strong>Telocytes (TCs) is a novel type of interstitial cells in many mammals organs, which participate in the organizational metabolism, mechanical support, immunomodulation and other aspects. The aim of this study was to explore the organizational chemical characteristics of TCs in pituitary gland and their changes in cryptorchid yaks.</p><p><strong>Methods: </strong>Transmission electron microscopy (TEM), toluidine blue staining, immunofluorescence, qRT-PCR, and Western blotting may enable us to understand TCs distribution characteristics and biological functions.</p><p><strong>Result: </strong>TEM confirmed the presence of TCs in the pituitary gland with small bodies and moniliform telopodes (Tps). The Tps extending out from the cell body to the peri-sinusoidal vessels spaces, the number of Tps is closely related to the morphology of the nucleus. The most obvious changes of TCs in the pituitary gland of cryptorchid yaks is the Tps are relatively shorter and decreased secretory vesicles. H.E. and toluidine blue staining revealed that TCs not only distributed between the sinusoidal blood vessels and the glandular cell clusters, but also present on the surface of vascular endothelial cells. The co-expression of TCs biomarkers, such as Vimentin/CD34, CD117/CD34 and α-SMA/CD34, were evaluated by immunofluorescence to further determine the phenotypic characteristics of TCs. Besides, we analyzed the mRNA and protein expression of these biomarkers to determine the characteristics of TCs changes and possible biological roles. Both the mRNA and protein expression of CD117 were significantly higher in the pituitary gland of cryptorchid yaks than in the normal (p < 0.01), the protein expression of CD34 in the cryptorchid yaks was significantly higher than the normal (p < 0.01). There were no significant difference in mRNA expression of Vimentin and α-SMA (p>0.05), while the protein expression were significantly increased in the normal yaks (p < 0.05).</p><p><strong>Conclusions: </strong>In summary, this study reports for the first time that the biological characteristics of TCs in yak pituitary gland. Although there is no significant change in the distribution characteristics, the changes in biological features of TCs in cryptorchid yaks are clear, suggesting that TCs participated in alteration in the local microenvironment of the pituitary gland. Therefore, our study provides clues for further investigating the role of TCs in the pituitary gland during the occurrence of cryptorchidism in yaks.</p>","PeriodicalId":9041,"journal":{"name":"BMC Veterinary Research","volume":"20 1","pages":"463"},"PeriodicalIF":2.3,"publicationDate":"2024-10-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11468414/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142406056","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Variation in the gut microbiota during the early developmental stages of common carp (Cyprinus carpio L.) and its correlation with feed and pond water microflora.","authors":"Jiahui Zhang, Yu Liu, Shijuan Shan, Cong Xu, Liguo An, Guiwen Yang, Lei Wang, Hua Li","doi":"10.1186/s12917-024-04321-3","DOIUrl":"10.1186/s12917-024-04321-3","url":null,"abstract":"<p><strong>Background: </strong>Fish gut microbiota undergo dynamic changes under the influence of many factors and play an important role in the nutrition, immunity and development in fish. Although common carp (Cyprinus carpio L.) is an economically important freshwater fish, there are few reports on its gut microbiota changes at different early developmental stages. In the present study, the gut microbiota of common carp during the early developmental stages and its correlation with the feed and pond water flora were studied using the Illumina MiSeq sequencing platform.</p><p><strong>Results: </strong>The results showed that the gut microbiota of common carp underwent continuous and mild changes over the development process, and the pond water environment might provide bacterial resources and have a certain influence on the changes in the gut microbiota of common carp. However, host selection pressure played a more important role in shaping the gut microbiota. Although the gut microbiota was affected by many factors, the presence of core microbiota indicated that some bacterial species adapt to the gut microenvironment of common carp and played a role in its growth process.</p><p><strong>Conclusions: </strong>The dynamic changes of gut microbiota of carp in early development stage were related to the feed, water environment and host selection. The results of this study provide a theoretical basis for healthy farming and disease prevention of common carp.</p>","PeriodicalId":9041,"journal":{"name":"BMC Veterinary Research","volume":"20 1","pages":"464"},"PeriodicalIF":2.3,"publicationDate":"2024-10-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11468302/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142406058","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparison of productive performance of Marandi, White Leghorn, and Marandi-White Leghorn crossbred chickens.","authors":"Somayeh Pourhamidi, Ali Esmailizadeh, Mohamad Salarmoini, Masood Asadi Fozi","doi":"10.1186/s12917-024-04314-2","DOIUrl":"10.1186/s12917-024-04314-2","url":null,"abstract":"<p><strong>Background: </strong>Recently, efforts like crossbreeding, strain crossing, and inbred hybridization have been performed to improve productive performance in native chickens. This experiment was carried out to evaluate the effects of crossbreeding on the improvement of the productivity of a native chicken. The White Leghorn exotic chicken (WL) as dam line and the Marandi indigenous chicken (MA) as sire line were used to generate the crossbreds (MA × WL). Body weight and egg production traits were measured in the three groups.</p><p><strong>Results: </strong>There are significant performance differences between WL and MA, with WL exhibiting superior body weight and egg production compared to MA (P < 0.05). Crossbreeding between WL and MA significantly improved most traits, with MA × WL outperforming MA. Negative heterosis was observed for body weight showing similarities between MA and MA × WL. Notably, MA × WL showed a closer resemblance to WL than MA in hen-day egg production at 40 and 44 weeks (P < 0.05).</p><p><strong>Conclusions: </strong>The results derived from this study show that the economic performance of native chickens can be enhanced through crossbreeding with exotic chickens. Using the crossbred chickens in rural areas can be more efficient than the native chickens. In addition, the resilience of the native chickens to the local environment can be transmitted genetically to the crossbred which should be investigated in further studies.</p>","PeriodicalId":9041,"journal":{"name":"BMC Veterinary Research","volume":"20 1","pages":"460"},"PeriodicalIF":2.3,"publicationDate":"2024-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11466034/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142399286","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of host breeds on gut microbiome and fecal metabolome in commercial pigs.","authors":"Sui Liufu, Kaiming Wang, Bohe Chen, Wenwu Chen, Xiaolin Liu, Sheng Wen, Xintong Li, Dong Xu, Haiming Ma","doi":"10.1186/s12917-024-04308-0","DOIUrl":"10.1186/s12917-024-04308-0","url":null,"abstract":"<p><strong>Background: </strong>Gut microbial composition and its metabolites are crucial for livestock production performance. Metabolite profiles from autopsied biospecimens provide vital information on the basic mechanisms that affect the overall health and production traits in livestock animals. However, the role of the host breed in the gut microbiome and fecal metabolome of commercial pigs remains unclear. In this work, differences in microbiota composition among three commercial pig breeds Duroc, Yorkshire, and Landrace were measured by 16S rRNA gene sequencing. Fecal metabolite compositions of the three pig breeds were detected using untargeted metabolomics.</p><p><strong>Results: </strong>There were significant differences in the gut microbiomes of the three species, indicating that host breed affects the diversity and structure of gut microbiota. Several breed-associated microorganisms were identified at different taxonomic levels. Notely, most microbial taxa were annotated as Lactobacillacea, Muribaculaceae, and Oscillospiraceae. Several bacteria, including Lactobacillus, Subdoligranulum, Faecalibacterium, Oscillospira, Oscillospiraceae_UCG-002, and Christensenellaceae_R-7_group, could be considered as biomarkers for improving the backfat thickness (BF) for commercial pigs. Additionally, KEGG analysis of gut microbiota further revealed that arginine biosynthesis, pyruvate metabolism, and fatty acid biosynthesis varied greatly among pig breeds. Multiple gut bacterial metabolites (e.g., spermidine, estradiol, and palmitic acid) were identified as breed-associated. Mediation analysis ultimately revealed the cross-talk among gut microbiota, metabolites, and BF thickness, proclaiming that the microbial and metabolic biomarkers identified in this study could be used as biomarkers for improving BF phenotype.</p><p><strong>Conclusions: </strong>This work provides vital insights into breed effects on gut microbiota and metabolite compositions of commercial pigs and uncovers potential biomarkers that are significant for pig breed improvement.</p>","PeriodicalId":9041,"journal":{"name":"BMC Veterinary Research","volume":"20 1","pages":"458"},"PeriodicalIF":2.3,"publicationDate":"2024-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11465751/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142399287","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"First molecular investigation to detect avian Mycoplasma species in clinical samples from laying-hen farms in Tunisia.","authors":"Soumaya Sabri, Lamia Khannous, Séverine Ferré, Adel Souissi, Radhouane Gdoura, Anne V Gautier-Bouchardon","doi":"10.1186/s12917-024-04311-5","DOIUrl":"10.1186/s12917-024-04311-5","url":null,"abstract":"<p><strong>Background: </strong>Avian mycoplasmas are known pathogens, which cause severe economic losses in poultry flocks. PCR is a rapid, sensitive, and less expensive diagnostic tool than culture for the identification of mycoplasmas in poultry farms. The objective of this study was to determine by PCR the presence of Mycoplasma spp., Mycoplasma gallisepticum (MG), Mycoplasma synoviae (MS), and Mycoplasma pullorum (MP) in laying hens located in the Sfax region, in the South of Tunisia.</p><p><strong>Results: </strong>A total of 781 tracheal swabs were collected from 13 laying-hen farms without clinical signs at the date of sampling. MP was detected by a newly described specific PCR assay. The prevalence calculated from PCR results at the flock level was 100% for Mycoplasma spp., 0% for MG, 84.6% for MS and 61.5% for MP. The overall prevalence at the animal level was 38.7% for Mycoplasma spp., 0% for MG, 25% for MS and 6.4% for MP. The overall prevalence of 100% of avian mycoplasmas in laying-hen farms (38.7% prevalence at the animal level) shows an alarming situation.</p><p><strong>Conclusions: </strong>These results underline the importance of monitoring the emergence and spread of Mycoplasma strains in farms in order to decrease economic losses due to mycoplasmoses.</p>","PeriodicalId":9041,"journal":{"name":"BMC Veterinary Research","volume":"20 1","pages":"459"},"PeriodicalIF":2.3,"publicationDate":"2024-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11465938/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142399288","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The comparative morphology of the oral cavity glands in captive South African painted dogs (Lycaon pictus pictus) and captive fennec foxes (Vulpes zerda) (Carnivora: Canidae).","authors":"Joanna Klećkowska-Nawrot, Krzysztof Stegmann, Arkadiusz Dziech, Gabriela Jędrszczyk, Igor Jucenco, Karolina Barszcz, Karolina Goździewska-Harłajczuk","doi":"10.1186/s12917-024-04305-3","DOIUrl":"10.1186/s12917-024-04305-3","url":null,"abstract":"<p><strong>Background: </strong>The African painted dog is classified as a relic canid lineage, inhabiting areas south of the Sahara. The fennec fox is the smallest member of the Canidae family, found in the Arabian Peninsula and northern Sahara.</p><p><strong>Methods: </strong>The gross anatomy and light microscopic examination of the oral cavity glands were studied in five adult captive South African painted dogs and five adult captive fennec foxes from the Wroclaw Zoological Garden, Poland. In this research, the zygomatic gland, monostomatic sublingual gland, polystomatic sublingual gland, mandibular gland, and parotid gland were examined for their topography, morphometry, histology, and histochemistry using hematoxylin and eosin, azan trichrome, mucicarmine, PAS, AB pH 1.0, AB pH 2.5, AB pH 2.5 PAS, and HID.</p><p><strong>Results: </strong>We found that the parotid glands were consistently the largest, followed by the mandibular and sublingual glands (both monostomatic and polystomatic). The zygomatic gland was the smallest in both South African painted dogs and fennec foxes. Interestingly, there were noticeable differences in the size, shape, and even composition of the secretory products between the two species. The zygomatic and polystomatic sublingual glands in the South African painted dog and the fennec fox were complex branched tubular. In the South African painted dog, the monostomatic gland was a branched tubular compound gland, while in the fennec fox, it was a branched tubuloalveolar compound gland. The mandibular gland in hunting dogs was a branched tubular compound gland, while in the fennec fox a branched tubuloalveolar compound gland. The parotid gland in the fennec fox was a branched acinar compound gland, whereas in the painted dog was a branched tubuloacinar.</p><p><strong>Conclusions: </strong>The basic structure of their glands is similar to that of other terrestrial carnivores, indicating a shared evolutionary origin and function. However, differences in the composition of their secretory products can reflect adaptations to their specific diets. This research provides valuable insights for veterinary medicine and underscores the importance of further studies. By analyzing wild canid populations and including a broader range of species with diverse diets, we could gain a deeper understanding of how diet influences salivary gland morphology within the Canidae.</p>","PeriodicalId":9041,"journal":{"name":"BMC Veterinary Research","volume":"20 1","pages":"457"},"PeriodicalIF":2.3,"publicationDate":"2024-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11465492/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142399289","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Immune modulatory effects of tulathromycin, gamithromycin, and oxytetracycline in cattle.","authors":"W Wheat, L Chow, K Still-Brooks, R Moore-Foster, J Herman, R Hunter, F Garry, S Dow","doi":"10.1186/s12917-024-04254-x","DOIUrl":"10.1186/s12917-024-04254-x","url":null,"abstract":"<p><p>Certain classes of antibiotics, including tetracyclines and macrolides, are known to exert immune suppressive effects in other species but the immune modulatory effects of these antibiotics have not been previously studied in cattle. To address this question, we investigated the effects of oxytetracycline, gamithromycin, and tulathromycin on T cell and macrophage responses to activation, using in vitro assays. In addition, we assessed the impact of these antibiotics on T cell responses in vivo following treatment of healthy cattle with currently recommended doses of each of the three antibiotics. We found that all 3 antibiotics markedly suppressed T cell proliferation in vitro at relevant therapeutic drug concentrations and significantly suppressed macrophage activation responses to LPS. In cattle treated with a single dose of each antibiotic, we observed significant suppression of T cell proliferation and cytokine production beginning as early as 6 h after administration, with increasing immune suppression observed at 48 h. Taken together, these results indicate that commonly used antibiotics in cattle exert significant immune modulatory activity, in addition to their antimicrobial activity. These off-target effects should be considered when using antibiotics for prophylaxis or metaphylaxis in high-risk dairy or beef cattle (192 words).</p>","PeriodicalId":9041,"journal":{"name":"BMC Veterinary Research","volume":"20 1","pages":"456"},"PeriodicalIF":2.3,"publicationDate":"2024-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11462805/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142387749","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}