BMC Veterinary Research最新文献

{"title":"Sub-bandage pressure changes in canine hindlimbs after re-application of custom rigid fiberglass splints.","authors":"Brandon Thibodaux, Kendra Eginton, Wanda Gordon-Evans","doi":"10.1186/s12917-024-04437-6","DOIUrl":"10.1186/s12917-024-04437-6","url":null,"abstract":"<p><strong>Background: </strong>While limited information is available, it is possible that high or low sub-bandage pressures cause injury with external coaptation. Fiberglass casting tape is a common splinting material that is custom made for the first bandage and reused in subsequent bandage changes. The aim of this preliminary study was to determine sub-bandage pressure changes in canine hindlimbs between initial splint placement and replacement of the bandage by a different person. The hypothesis was that there would be a clinically meaningful change in sub-bandage pressures with reapplication of the splint in at least one location. Sub-bandage pressures were measured at five different anatomic locations on each of 11 canine hind limbs with dogs standing and in lateral recumbency after customizing a fiberglass lateral tarsal splint for each dog. A second clinician then reapplied the bandage, reusing the same fiberglass splint. Second bandages failed if there was a change in pressure of 20 mmHg at any location.</p><p><strong>Results: </strong>Ten of 11 bandages failed (90%). All but one location failed due to increases in pressure. There were significant changes between lateral recumbency and standing pressures, but there was no consistent pattern of these changes among the dogs.</p><p><strong>Conclusion: </strong>There are changes in sub-bandage pressures when the splint is reused in 90% of bandages. In addition, changes in pressure occur unpredictably when transitioning from lateral recumbency to standing. Further study of the relationship of sub-bandage pressures to bandage complications is vital to help establish safe ranges and inform bandaging improvements.</p>","PeriodicalId":9041,"journal":{"name":"BMC Veterinary Research","volume":"20 1","pages":"574"},"PeriodicalIF":2.3,"publicationDate":"2024-12-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11664813/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142875863","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Population pharmacokinetics modelling for clinical dose adjustment of carboplatin in dogs.","authors":"Jérémy Béguin, Sarra Mahfoudhi, Marie Uzel, Antoine Rostang, Catherine Ibish, Aude A Ferran, Ludovic Pelligand, Anne Hulin, Matthias Kohlhauer","doi":"10.1186/s12917-024-04404-1","DOIUrl":"10.1186/s12917-024-04404-1","url":null,"abstract":"<p><strong>Background: </strong>Carboplatin is a human chemotherapeutic agent which is frequently used in dogs for the management of solid tumors. In human patient, its dosage is adjusted carefully, based on the creatinine clearance computation. In dogs however, the pharmacokinetics of carboplatin is poorly known and the dose 300 mg/m2 is based mostly on empirical data. Here, we aimed at characterizing the pharmacokinetics of carboplatin and determined the influence of several covariates, including creatinine plasma concentration and neutering status, in dogs, and used this model to predict myelotoxicity.</p><p><strong>Results: </strong>Sixteen client owned dogs were included after carboplatin administration (300 mg/m²). For each animals, three to four plasma samples were collected and free plasma concentration of carboplatin was determined by HPLC/MS and analysed using Monolix® software with Non-linear mixed effect modelling. A mono-compartmental model best described the plasma concentration of carboplatin with log plasma creatinine concentration and sterilization status as covariates. After adjustment with the covariates, median population clearance was 3.62 [3.15 - 4.12] L/h/kg and volume of distribution was 3.93 [3.84 - 4.14] L/kg. The application of this model in 14 additional dogs demonstrates that individual drug exposure (model-predicted Area Under the Curve) predicted thrombocyte blood reduction (Pearson coefficient r² = 0.73, p = 0.002) better than dose after 14 days following administration of carboplatin.</p><p><strong>Conclusion: </strong>Based on our results, plasma creatinine concentration and the sterilization status are relevant explanatory covariates for the pharmacokinetics variability of carboplatin in client owned dogs. Dose adjustment based on these parameters could represent a promising strategy for minimizing thrombocyte toxicity.</p>","PeriodicalId":9041,"journal":{"name":"BMC Veterinary Research","volume":"20 1","pages":"575"},"PeriodicalIF":2.3,"publicationDate":"2024-12-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11664934/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142881206","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Spatial distribution of Q fever in sheep and goats of selective villages of Punjab Province, Pakistan.","authors":"Freeha Amin, Shahzad Ali, Ahmad Hassan, Imran Rashid, Heinrich Neubauer, Katja Mertens-Scholz","doi":"10.1186/s12917-024-04421-0","DOIUrl":"10.1186/s12917-024-04421-0","url":null,"abstract":"<p><p>This study aimed to assess the geographical distribution of Q fever in sheep and goats in different areas of Punjab, Pakistan. Three hundred blood samples of small ruminants including sheep and goats were collected from 60 villages of three districts (Okara, Kasur, and Pakpattan) of Punjab Province Pakistan and tested for the detection of anti-Coxiella burnetii antibodies using a commercial Indirect ELISA kit. Data related to sampling location, host species, gender, age, and GPS coordinates were collected for spatial analysis. A surface plot was created using inverse distance weight (IDW) by interpolation of the Aeronautical Reconnaissance Coverage Geographic Information system (Arc GIS). The district Kasur (14%) and the tehsil Chunian (24%) had the most prominent Q fever prevalence in both species. No ovine males were seropositive but 19.2% of male goats were seropositive. No samples of sheep younger than 1 year were found seropositive. Gender in sheep and age in goats have to be considered as significant risk factors based on multiple logistic regression analysis. Based on spatial analysis, seropositivity for C. burnetii antibodies was more likely observed in villages of tehsil Kasur, Pattoki, Okara, Depalpur, and Renala Khurd in the case of goats while in case of sheep villages of tehsil Chunian, Renala Khurd and Pakpattan. The main outcome of this study is that Q fever-specific antibodies of C. burnetii are prevalent in the goat and sheep populations of the study area and we have identified potential risk zones. The findings of this study can be used for the control of Q fever in small ruminants of the study area to minimize the risk of this zoonosis in other animals and the associated human population.</p>","PeriodicalId":9041,"journal":{"name":"BMC Veterinary Research","volume":"20 1","pages":"577"},"PeriodicalIF":2.3,"publicationDate":"2024-12-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11665226/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142881209","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Early pregnancy diagnosis in cows using corpus luteum blood flow analysis based on colour Doppler ultrasonography and mRNA analysis.","authors":"Malshani De Silva, Devinda Wickramasingha, Nirodha Abayalath, Basil Alexander, Missaka Wijayagunawardane, Kai Fai Lee, Suranga Kodithuwakku","doi":"10.1186/s12917-024-04438-5","DOIUrl":"10.1186/s12917-024-04438-5","url":null,"abstract":"<p><strong>Background: </strong>Reproductive efficiency is paramount in the dairy industry, where early pregnancy detection of dairy cows will allow to detect the non-pregnant animals early, thus enabling to re-synchronize them and getting them pregnant leading to decrease in calving interval, which, in turn, is critical for maximizing productivity and economic gain. The objective of this study was to evaluate the colour Doppler ultrasonography (CDUS) and peripheral blood leukocytes (PBLs)-based pregnancy-associated biomarker mRNAs expression for the earliest detection of pregnancy status in the dairy cows at post insemination. Intensively managed animals were ovulation synchronized and subjected to timed artificial insemination (TAI). On day 20, corpus luteum blood flow (CLBF) was evaluated using CDUS in 30 cows. The percentage of the incoming blood flow (as an area) of the corpus luteum (CL) was determined using an image analysis software. On day 35, the same operator performed a final pregnancy diagnosis using transrectal ultrasonography to confirm the pregnancy. Blood samples were collected on day 20 and 28 after TAI for biomarkers analysis. The mRNA expression levels of ISG15, MX1, MX2, and PAG9 genes in PBLs were determined by quantitative polymerase chain reaction (qPCR).</p><p><strong>Results: </strong>The identified CLBF cutoff point resulted 100% sensitivity and negative predictive value (NPV) in determining non-pregnant status on day 20 in the cows. Overall, MX2 and ISG15 mRNAs showed the most significant (P < 0.05) expression levels in pregnant animals on day 20 and 28 compared to non-pregnant animals. Among them, MX2 showed the highest expression levels on both days, ascertaining it as the better candidate biomarker for the earliest identification of pregnancy.</p><p><strong>Conclusions: </strong>The CDUS-based CLBF analysis on day 20 after TAI can be potentially used for the early identification of non-pregnancy status in dairy cows and MX2 could be a potential mRNA candidate for the identification of pregnancy in cows. Further studies should be conducted in large scale to validate these findings due to the small sample number used in the current study.</p>","PeriodicalId":9041,"journal":{"name":"BMC Veterinary Research","volume":"20 1","pages":"572"},"PeriodicalIF":2.3,"publicationDate":"2024-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11661188/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142871265","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antimicrobial resistance of Escherichia coli in cats and their drinking water: drug resistance profiles and antimicrobial-resistant genes.","authors":"Panpicha Sattasathuchana, Suttiporn Srikullabutr, Anusak Kerdsin, Sathidpak Nantasanti Assawarachan, Patamabhorn Amavisit, Win Surachetpong, Naris Thengchaisri","doi":"10.1186/s12917-024-04435-8","DOIUrl":"10.1186/s12917-024-04435-8","url":null,"abstract":"<p><strong>Background: </strong>Antimicrobial resistance (AMR) is a global health concern that is exacerbated by the transmission of bacteria and genetic material between humans, animals and the environment. This study investigated AMR of Escherichia coli (E. coli) isolated from cats' feces and their drinking water. The study compared the AMR of fecal and environmental E. coli isolates from pet cats.</p><p><strong>Results: </strong>A total of 104 samples (52 cat feces and 52 cat drinking water samples) was cultured for E. coli. The study compared the AMR of fecal and environmental E. coli isolates from pet cats. An analysis of carbapenemase and extended-spectrum β-lactamase (ESBL)-producing E. coli genes (bla_TEM, bla_SHV and bla_CTX-M) and phylogroups of E. coli was also performed. E. coli was identified from all fecal (100%) and almost half of drinking water (44.2%) samples. All E. coli isolate was susceptible to amikacin or imipenem. Clindamycin showed the highest resistance rate. β-lactam was the most found with co-resistance profiles, comprising β-lactams with aminoglycosides, quinolones, sulfonamides, macrolides or carbapenems. Very strong positive correlations of bactericidal agents were found among quinolones (r > 0.8, p < 0.01). Within the group of bacteriostatic agents, moderate correlation was observed between azithromycin and sulfa-trimethoprim (r = 0.5253, p < 0.01). Carbapenemase gene was not detected in this study. Extended-spectrum β-lactamase-producing E. coli genes (bla_TEM, bla_SHV and bla_CTX-M) were identified in E. coli isolates, with bla_TEM being the most predominant. Furthermore, phylogroup B2 was the dominant segregation among the E. coli, particularly in fecal isolates.</p><p><strong>Conclusions: </strong>This study identified AMRin E. coli isolated from cats' feces and their drinking water.  The results revealed that the phylogroup B2 was predominant, with bla_TEM being the most widespread ESBL gene.</p>","PeriodicalId":9041,"journal":{"name":"BMC Veterinary Research","volume":"20 1","pages":"573"},"PeriodicalIF":2.3,"publicationDate":"2024-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11660686/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142871319","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Investigation of growth traits in Turkish Merino lambs using multi-locus GWAS approaches: Middle Anatolian Merino.","authors":"Yalçın Yaman, Şükrü Doğan, Mesut Kirbaş, A Taner Önaldi, Yavuz Kal","doi":"10.1186/s12917-024-04428-7","DOIUrl":"10.1186/s12917-024-04428-7","url":null,"abstract":"<p><p>This study explored the genetic basis of growth traits in Middle Anatolian Merino lambs using multi-locus genome-wide association study (GWAS) analyses. Descriptive statistics indicated moderate heritability (h² = 0.363) for birth weight (BW) and (h² = 0.309) for weaning weight (WW), both statistically significant (p < 0.001). Strong genetic correlations were observed between WW and BW (rG = 0.922) and WW and Kleiber ratio (KR, rG = 0.896), implying that simultaneous improvements may be possible through targeted selection. Five multi-locus methods (mrMLM, FASTmrMLM, pLARmEB, FASTmrEMMA, and ISIS EM-BLASSO) were used to identify the polygenic basis of the traits. For BW and WW, 20 and 18 significant SNPs (LOD ≥ 5) were detected, respectively, with some SNPs co-detected by multiple methods. In contrast, only 10 significant SNPs were identified for KR, all exclusively by the ISIS EM-BLASSO approach. Pathway analyses within ± 100 Kb of associated SNPs revealed genes and pathways influencing these traits, which could be leveraged in future breeding programs for enhanced growth performance. The identified SNPs, particularly those associated with BW and WW traits, could facilitate genomic selection if validated in larger and more diverse populations.</p>","PeriodicalId":9041,"journal":{"name":"BMC Veterinary Research","volume":"20 1","pages":"567"},"PeriodicalIF":2.3,"publicationDate":"2024-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11658107/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142852548","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Serum metabolic alterations in chickens upon infectious bursal disease virus infection.","authors":"Dan Wang, Jiangwei Song, Jing Wang, Rong Quan","doi":"10.1186/s12917-024-04402-3","DOIUrl":"10.1186/s12917-024-04402-3","url":null,"abstract":"<p><strong>Background: </strong>Infectious bursal disease virus (IBDV) is a highly contagious immunosuppressive virus of chickens. Chickens acquire infection by the oral route under natural conditions. Although the histological and pathological changes after IBDV infection are well described, the alterations in serum metabolome have not been reported. In this study, SPF chickens were infected with attenuated IBDV (atIBDV) strain LM and very virulent IBDV (vvIBDV) strain LX, respectively. On the seventh day after oral infection, serum samples of experimental chickens were identified using ultra-high performance liquid chromatography-MS/MS (UHPLC-MS/MS). The serum metabolic profiles were analyzed by multivariate statistical methods. KEGG enrichment analysis was performed to evaluate the dysregulated biological pathways.</p><p><strong>Results: </strong>We identified 368 significantly altered metabolites in response to both atIBDV and vvIBDV infection. The metabolic disorder of amino acid and lipid was associated with IBDV infection, especially tryptophan, glycerophospholipid, lysine, and tyrosine metabolism. The differential metabolites enriched in the four metabolic pathways were PC(20:4(5Z,8Z,11Z,14Z)/18:0), PE(16:0/18:2(9Z,12Z)), PE(16:0/22:6(4Z,7Z,10Z,13Z,16Z,19Z)), PE(18:0/20:4(5Z,8Z,11Z,14Z)), PE(18:0/20:4(8Z,11Z,14Z,17Z)), PE(18:0/22:6(4Z,7Z,10Z,13Z,16Z,19Z)), PE(20:3(8Z,11Z,14Z)/16:0), PE(22:6(4Z,7Z,10Z,13Z,16Z,19Z)/16:0), PE-NMe(20:5(5Z,8Z,11Z,14Z,17Z)/18:0), PS(20:3(5Z,8Z,11Z)/18:2(9Z,12Z)), 2-aminobenzoic acid, 4-(2-aminophenyl)-2,4-dioxobutanoic acid, N-acetylserotonin, 5-hydroxyindoleacetate, indole-3-acetaldehyde, indole-3-acetate, p-coumaric acid, L-tyrosine, homovanillin, and S-glutaryldihydrolipoamide.</p><p><strong>Conclusion: </strong>The atIBDV and vvIBDV infection causes metabolic changes in chicken serum. The differential metabolites and dysregulated metabolic pathways reflect the host response to the IBDV infection.</p>","PeriodicalId":9041,"journal":{"name":"BMC Veterinary Research","volume":"20 1","pages":"569"},"PeriodicalIF":2.3,"publicationDate":"2024-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11657878/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142852574","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Shaken, not stirred: magnetic bead DNA extraction as a rapid and effective method for the scaling up of bovine tuberculosis diagnosis.","authors":"V Lorente-Leal, A Gomez-Buendia, A Gutiérrez-Tobaruela, L de Juan, J Bezos, B Romero","doi":"10.1186/s12917-024-04427-8","DOIUrl":"10.1186/s12917-024-04427-8","url":null,"abstract":"<p><strong>Background: </strong>The growing use of real-time PCR (qPCR) as a diagnostic method for bovine TB (bTB) requires rapid and effective DNA extraction methods, which are crucial for its success. Automated DNA extraction methods based on magnetic beads are a promising alternative to conventional silica column-based protocols (COL protocol) due to their high throughput capacity and reduced hands-on time. This study aimed to assess the performance of the MagMax CORE Nucleic Acid Purification kit and the KingFisher Flex instrument (KF protocol) as an alternative for scaling up the use of qPCR in bTB diagnosis.</p><p><strong>Methodology: </strong>Performance was evaluated with two different real-time PCR (qPCR) protocols, based on the IS6110 element and the QuantiFast and VetMAX™ (QF and VM protocols) kits, on 145 frozen tissue homogenates confirmed as either bTB-positive or negative through a composite reference standard based on microbiological culture, column-based extraction, and qPCR, as well as on negative tissue samples spiked with 10⁶ to 10³ CFU/ml of M. bovis BCG.</p><p><strong>Results: </strong>The performance of both qPCR protocols was very high on samples extracted using the KF protocol, with positive percent agreement (PPA) values of 89.04% [95% Confidence Interval (CI): 79.54-95.15%] and 93.15% [95% CI: 84.74-97.74%] for the QF and VM protocols, respectively, and negative percent agreement (NPA) values of 100% [95% CI: 95.01-100.00%]. A higher variability was identified in samples analysed with the same qPCR protocol but different extraction methods. Higher Ct values were identified for samples extracted using the KF protocol in both routine and spiked samples, likely due to using the same amount of starting material for both extraction methods, which was lower than recommended by the manufacturer for the KF protocol.</p><p><strong>Discussion: </strong>The results of this study indicate that the MagMAX CORE Nucleic Acid Purification kit coupled with a KingFisher Flex instrument is a valuable alternative for the extraction of MTBC DNA from bovine tissues. However, the increased variability and Ct values suggest that a larger amount of starting material is recommended for this methodology, warranting further studies.</p>","PeriodicalId":9041,"journal":{"name":"BMC Veterinary Research","volume":"20 1","pages":"568"},"PeriodicalIF":2.3,"publicationDate":"2024-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11657575/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142852577","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The cow GUTBIOME CY study: investigating the composition of the cattle gut microbiome in health and infectious disease transmission in cyprus.","authors":"Daphne E Mavrides, Maria Liapi, Despo Ierodiakonou, Christodoulos Pipis, Stavros Malas, Eleni Gentekaki, Anastasios D Tsaousis","doi":"10.1186/s12917-024-04419-8","DOIUrl":"10.1186/s12917-024-04419-8","url":null,"abstract":"<p><strong>Background: </strong>Recent evidence suggests that the lower gut microbiome of ruminants presents roles in their health and environment, including the development of the mucosal immune system, milk production efficiency and quality and subsequent methane emissions. However, there are proportionately fewer studies on this complex microbial community in cattle and region-focus studies are non- existent.</p><p><strong>Methods: </strong>Herein, we present the research protocol of the GUTBIOME CY project pertaining to determine the composition of the lower gut microbiome in dairy cows situated in 37 farms across five districts of the island of Cyprus. Detailed questionnaires on animal husbandry and farming practices will be gathered from each farm. Faecal, milk (individual and bulk) and water samples will also be collected from cows and their offspring. Samples will be analysed using a combination of molecular biology and bioinformatics pipelines to define microbiome profiles and antimicrobial resistance (AMR). Information collected from the questionnaires will be used to test for associations between animal husbandry or farming practices and microbiome components and AMR.</p><p><strong>Discussion: </strong>Collected samples will establish the first dairy cattle biobank in the country for contributing substantially towards scientific advancements in microbiome research and providing insights to all stakeholders, tailored to the unique agricultural context of Cyprus.</p>","PeriodicalId":9041,"journal":{"name":"BMC Veterinary Research","volume":"20 1","pages":"566"},"PeriodicalIF":2.3,"publicationDate":"2024-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11657508/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142852582","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Investigation on parasite infection and anthelmintic resistance of gastrointestinal nematodes in sheep in Hinggan league (City), China.","authors":"Yanmin Zhang, Weijie Wu, Zhiming Bai, Hao Zhang, Hao Liu, Lili Zhang, Chagan Luo, Mulan Chen, Jing Lu, Wa Gao, Wenlong Wang, Chunxia Liu","doi":"10.1186/s12917-024-04420-1","DOIUrl":"10.1186/s12917-024-04420-1","url":null,"abstract":"<p><strong>Background: </strong>Different areas of sheep infected with different types of parasites, all will cause serious harm to the local sheep, and the widespread use and repeated use of anthelmintics have produced different degrees of anthelmintic resistance (AR) in various regions. We re-investigated the infection of common parasites and AR of Gastrointestinal Nematodes (GINs) in sheep in Horqin Right Wing Front Banner, and first investigated the common parasite types and AR of GINs in sheep at other four areas in Hinggan league (city), China.</p><p><strong>Results: </strong>A total of 1770 fecal samples were collected from 1 prefecture-level city and 4 counties in Hinggan league, in which the infection rate of Coccidia ranged from 83.3% to 96.06%, that of Ascaris ovis ranged from 10.17% to 15.19%, that of Moniezia benedeni ranged from 0.6% to 1%, that of Moniezia expansa ranged from 0.33% to 8.15%. The infection rate of GINs was 100%, and Haemonchus contortus was still the dominant species. The AR results showed that only the closantel in Horqin Right Wing Middle Banner was low resiatant, and the other three regions had been resistant. Levamisole also occurred AR in the other four regions, the widely used ivermectin and albendazole had produced serious AR in five areas. The research shows that GINs are becoming more and more resistant to various anthelmintics, which has made the problem worse.</p><p><strong>Conclusions: </strong>Understanding the dynamic changes of parasite infections and the development trend of AR in sheep in the region in this paper, the development trend seems to be more serious than imagined. Therefore only by deeply understanding the parasitic infections of sheep in this land can more reasonable medication guidance be carried out. It is expected to provide new ideas formore innovative, scientific and sustainable methods of preventing and controlling parasites.</p>","PeriodicalId":9041,"journal":{"name":"BMC Veterinary Research","volume":"20 1","pages":"564"},"PeriodicalIF":2.3,"publicationDate":"2024-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11657611/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142852550","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}