BMC Veterinary Research最新文献

{"title":"Knowledge of companion animals' practitionerson stem-cell based therapies in a clinical context: a questionnaire-based survey in Portugal.","authors":"Rafael S Lopes, João Requicha, Nuno Carolino, Eduardo Costa, Pedro Carvalho","doi":"10.1186/s12917-025-04872-z","DOIUrl":"10.1186/s12917-025-04872-z","url":null,"abstract":"<p><p>Stem cells therapies are a promising therapeutic option, and although not yet widely used in daily clinical practice in companion animals, they have shown great potential in the treatment of a variety of diseases. This study aims to investigate the individual profile of Portuguese veterinary practitioners, their knowledge about this type of therapy and their interest in its future use and application. This questionnaire-based descriptive study was carried out in Portugal from November 2021 to January 2022. A questionnaire-based survey was conducted online, using an electronic platform. The following topics were surveyed: (i) profile of veterinarians; (ii) knowledge about cell therapies; and (iii) interest in future use and application. After an internal validation, the survey was released online for three months in Portuguese veterinary social network groups. Data were collected and descriptive analysis and Chi-square test were performed. A total of 275 responses were obtained and analysed. Analysis of the results showed that the participating veterinarians know what mesenchymal stromal cells (MSCs) are (90.9%) and are generally aware of their therapeutic potential (94.5%). Regarding their use, 56.7% of the respondents do not know their application in veterinary pathologies. The remaining 43.3% indicated the situations in which they knew of its use. Orthopaedic and dental or oral diseases were the most frequently mentioned. Regarding the immunological effect of MSCs, there is a great lack of information. Data analysis showed that 72.4% of respondents were unaware of the possibility of an immunogenic response following their administration. However, 53.5% stated that they were aware of the positive immunomodulatory capacity. Most professionals who participated in this survey consider this topic relevant in veterinary medicine (95.6%), as well as the existence of this type of service in the national territory (96.4%). The great interest shown by 96.7% of the respondents in learning more about the subject highlights the importance of raising awareness, conducting further studies and promoting knowledge sharing in their daily clinical practice and research. The relationship between the national status of stem-cell therapy and veterinarians' knowledge is influenced by limited regulation but growing professional interest and driven by private companies and professional organisations. This study contributes to a better understanding of the trends related to this topic in the context of veterinary practice in Portugal.</p>","PeriodicalId":9041,"journal":{"name":"BMC Veterinary Research","volume":"21 1","pages":"487"},"PeriodicalIF":2.6,"publicationDate":"2025-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12288361/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144697549","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Total mixed ration enhances nutrient digestibility, blood biochemical parameters and faecal microbial diversity in horses.","authors":"Hongxin Jing, Pengshun Liu, Shengchen Zheng, Ketao Cao, Xiaobin Li","doi":"10.1186/s12917-025-04937-z","DOIUrl":"10.1186/s12917-025-04937-z","url":null,"abstract":"","PeriodicalId":9041,"journal":{"name":"BMC Veterinary Research","volume":"21 1","pages":"483"},"PeriodicalIF":2.6,"publicationDate":"2025-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12285169/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144688877","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pharmacokinetic study of voriconazole administered orally at 50 mg/kg in nursehound sharks (Scyliorhinus stellaris) and udulate skates (Raja undulata).","authors":"Daniela Cañizares-Cooz, Daniel García-Párraga, Teresa Álvaro-Álvarez, Sonia Rubio-Langre, Teresa Encinas, Pablo Morón-Elorza","doi":"10.1186/s12917-025-04930-6","DOIUrl":"10.1186/s12917-025-04930-6","url":null,"abstract":"<p><strong>Background: </strong>Fungal diseases represent an ongoing problem for elasmobranchs kept under human care worldwide. When present, mycoses often lead to high mortality rates, compromising welfare and conservation programs in aquariums. Various fungal species have been identified as causative agents, with Fusarium solani complex most frequently being isolated. Treatment remains unclear; however, recent clinical reports have shown positive outcomes after the administration of azole antifungal drugs to elasmobranchs affected by different fungal infections, specifically for voriconazole. Clinical cases report different dose regimens and administration routes, and pharmacokinetic (PK) studies in elasmobranchs are needed. For this purpose, a PK study was performed and voriconazole was administered at 50 mg/kg PO to six nursehound sharks (Scyliorhinus stellaris) and six undulate skates (Raja undulata). Plasma concentrations were determined using a validated high-performance liquid chromatography (HPLC) method, and PK parameters were estimated using a non-compartmental model.</p><p><strong>Results: </strong>The mean peak plasma concentration (C_max) ± standard error of the mean (SEM) after oral administration of 50 mg/kg of voriconazole was 3.32 ± 0.92 µg/ml in nursehound sharks and 10.53 ± 0.91 µg/ml in undulate skates. Terminal-half lives for sharks and skates resulted 113.43 ± 29.45 h and 9.88 ± 1.33 h, respectively. Plasma concentrations were above MIC₅₀ in vitro values for Fusarium solani for 54 to 72 h.</p><p><strong>Conclusions: </strong>These findings suggest that voriconazole persists longer in sharks due to extended half-life, whereas in skates, the drug reaches higher plasma concentrations but is eliminated more rapidly. These interspecies variations should be considered when selecting the oral administration route for treatment and determining the appropriate posology regimens for antifungal therapy in elasmobranchs.</p>","PeriodicalId":9041,"journal":{"name":"BMC Veterinary Research","volume":"21 1","pages":"484"},"PeriodicalIF":2.6,"publicationDate":"2025-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12285040/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144697550","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dietary supplementation of polysaccharides from Millettia speciosa Champ. ex Benth on growth performance, immune function, antioxidant abilities and intestinal health of Wenchang chickens.","authors":"Yu-Hang Liu, Jie Liu, Xin Feng, Quan-Wei Liu, Rui-Ping Sun, Wei Wu, Kun Ouyang, Jing-Li Yuan, Yan Zhang, Xiu-Ping Wang, Gui-Ping Zhao, Li-Min Wei","doi":"10.1186/s12917-025-04934-2","DOIUrl":"10.1186/s12917-025-04934-2","url":null,"abstract":"<p><p>This study aims to systematically evaluate the effects of Millettia speciosa Champ. ex Benth polysaccharides (MSCP) on broiler chickens through comprehensive assessment of growth performance parameters, immune system modulation, and intestinal microbial community dynamics. A total of 576 healthy 80-day-old Wenchang chickens were randomly assigned to six experimental groups in a completely randomized design. The control group (Control) received a basal diet, while the antibiotic-treated group was supplemented with 2 g/kg chlortetracycline hydrochloride (CTC) as a positive control. Four experimental groups were supplemented with different concentrations of MSCP: 400 mg/kg (MSCP₄₀₀), 800 mg/kg (MSCP₈₀₀), 1600 mg/kg (MSCP₁₆₀₀), and 3200 mg/kg (MSCP₃₂₀₀). The study employed a replicated pen design with 8 replicates per treatment group, each containing 12 birds. The results showed that dietary MSCP significantly increased the final body weight (P < 0.001) and average daily gain of chickens (P = 0.017), particularly at a dosage of 800 mg/kg. We also found that serum CAT level (P = 0.030) and GSH-Px level (P = 0.011) were significantly higher in the MSCP group compared to the CTC group. Adding MSCP to the feed raised serum IL-4 level (P = 0.016) and significantly increased IgA level (P = 0.047) at 1600 mg/kg. MSCP at 400 and 800 mg/kg significantly increased villus height in the duodenum, jejunum, and ileum (P < 0.001 for duodenum and jejunum; P = 0.040 for ileum). Moreover, MSCP also increased the relative abundance of beneficial bacteria Synergistota, Parabacteroides, Megamonas and Faecalibacterium, and increased the diversity of intestinal flora. To sum up, adding MSCP to feed had a positive impact on production performance, comparable to antibiotics, particularly at a dosage of 800 mg/kg. This suggests that MSCP is a promising, safe, and effective alternative to antibiotics in feed additives.</p>","PeriodicalId":9041,"journal":{"name":"BMC Veterinary Research","volume":"21 1","pages":"485"},"PeriodicalIF":2.6,"publicationDate":"2025-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12285003/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144697548","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sarcocystis species: molecular identification and seroprevalence in water buffaloes (Bubalus bubalis).","authors":"Nagwa I Toaleb, Raafat M Shaapan, Haitham Elaadli, Kadria N Abdel Megeed, Dina Aboelsoued","doi":"10.1186/s12917-025-04933-3","DOIUrl":"10.1186/s12917-025-04933-3","url":null,"abstract":"<p><p>Meat infection with the coccidian protozoan zoonotic Sarcocystis spp. causes public health hazards and high economic loss. The current study aimed to investigate molecular identification, cyst histological examination and seroprevalence of Sarcocystis spp. in water buffaloes slaughtered in the main abattoirs of 6 Egyptian Governorates; Cairo, Giza, Beni-suef, Al-Sharqia, Qalyubia, and El-Beheira. Each buffalo was visually inspected for the presence of Sarcocystis macrocysts and blood samples were collected. Out of 900 examined water buffaloes (Bubalus bubalis), 246 (27.3%) were found to be infected based on macroscopic examination. Histological examination of the macrocyst revealed metrocytes close to the cyst's edge and elongated curving basophilic bradyzoites occupying most of the cyst. Depending on age, we categorized naturally infected buffaloes into 3 groups: young (< 2 y.), adult (2-5 y.) and old (5-10 y.). The highest infection rate was observed in older buffaloes aged 5-10 years, meanwhile female animals exhibited a higher prevalence of infection compared to males. The esophagus had the highest presence of Sarcocystis compared to other organs. Using PCR based on 18S rRNA gene and sequencing, we isolated 3 Sarcocystis species from infected tissues: S. buffalonis, S. fusiformis and S. hirsuta-like. The prepared whole cyst antigen of S. fusiformis was characterized by 9 major polypeptides (140, 120, 78, 66, 53, 39, 32, 24 and 19 kDa) on 10% SDS-PAGE. Using western blotting, we identified 2 common immunogenic reactive bands (66 and 32 kDa) against naturally infected buffalo sera and hyperimmune rat sera. Additionally, sarcocystosis positive seroprevalence rate was 46.4% (418/900) with a sensitivity of 97.6% and specificity of 100% using indirect ELISA based on S. fusiformis whole cyst antigen. In conclusion, this study highlights the molecular identification, cyst histological examination and seroprevalence of Sarcocystis sp. among buffaloes in Egypt. ELISA using the S. fusiformis whole cyst antigen could be adapted to detect antibodies to Sarcocystis sp., in live animals, with an acceptable specificity and sensitivity.</p>","PeriodicalId":9041,"journal":{"name":"BMC Veterinary Research","volume":"21 1","pages":"482"},"PeriodicalIF":2.6,"publicationDate":"2025-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12281994/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144688876","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of long-term high-dose prednisolone in Beagle dogs on structural and functional responses of the HPA Axis.","authors":"Felicitas S Boretti, Livia Harburger, Barbara Riond, Elisabeth Neubert, Regina Hofmann-Lehmann, Stefan Unterer, Matthias Dennler, Nadja S Sieber-Ruckstuhl","doi":"10.1186/s12917-025-04876-9","DOIUrl":"10.1186/s12917-025-04876-9","url":null,"abstract":"","PeriodicalId":9041,"journal":{"name":"BMC Veterinary Research","volume":"21 1","pages":"480"},"PeriodicalIF":2.6,"publicationDate":"2025-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12281723/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144688874","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Immunologic response to first booster vaccination in dogs treated with zenrelia™ (ilunocitinib tablets) at up to three times the recommended therapeutic dose compared to untreated controls.","authors":"Genevieve M Fent, Jay Jacela, Rodrigo Plazola-Ortiz, Jeremiah Olps, Erin E McCandless, Céline E Toutain, Sandra O'Kelley, Stephen King","doi":"10.1186/s12917-025-04929-z","DOIUrl":"10.1186/s12917-025-04929-z","url":null,"abstract":"<p><strong>Background: </strong>This prospective, non-inferiority study was designed to evaluate the serologic response to booster vaccination in dogs previously vaccinated according to standard of care practice and treated for 56 days with up to 3X the recommended label dose of ilunocitinib. Vaccination occurred 28 days post-treatment initiation and continued through day 56. Measured study parameters included: vaccine-induced serum antibody titers at 15- and 28-day post-vaccination, along with hematological and clinical chemistry parameters.</p><p><strong>Results: </strong>Serum antibody titer levels were not significantly different between the control and ilunocitinib treated groups (1X and 3X), and both met the non-inferiority criteria versus the control group after 56 days of treatment (SD 56). All dogs administered ilunocitinib had titers at or above threshold for rabies, CAV-2, and CPV at 15- and 28-day post-vaccination timepoints (SD 43 and SD 56, respectively). The percentage of dogs with titers at or above threshold on SD 43 for CDV were 96, 100, and 95% in the control, 1X and 3X groups, respectively. By SD 56, the percentage of animals with titers above threshold for CDV were similar to pre-vaccination levels in all groups.</p><p><strong>Conclusion: </strong>This study demonstrated treatment with ilunocitinib at 1X or 3X the therapeutic dose for 56 days did not significantly attenuate the serologic response to CAV-2, CPV, CDV or rabies booster vaccinations compared to untreated control animals. All animals remained clinically healthy during the study, with only mild gastrointestinal or skin abnormalities typical of laboratory dogs or JAK inhibitor therapy.</p>","PeriodicalId":9041,"journal":{"name":"BMC Veterinary Research","volume":"21 1","pages":"481"},"PeriodicalIF":2.6,"publicationDate":"2025-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12281846/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144688875","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular epidemiology and genetic characterization of PCV2 and PCV3 circulating in domestic pigs and wild boars in central-southern regions of Italy.","authors":"Irene Melegari, Shadia Berjaoui, Barbara Secondini, Valentina Curini, Valeria Di Lollo, Umberto Molini, Alessio Lorusso, Giovanni Franzo","doi":"10.1186/s12917-025-04928-0","DOIUrl":"10.1186/s12917-025-04928-0","url":null,"abstract":"","PeriodicalId":9041,"journal":{"name":"BMC Veterinary Research","volume":"21 1","pages":"478"},"PeriodicalIF":2.3,"publicationDate":"2025-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12278492/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144673839","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Lactobacilli-based postbiotic differentially affects chicken macrophage-like HD11 cells depending on stimulatory lipopolysaccharide dosage.","authors":"Samuel C G Jansseune, Jürgen van Baal, Fany Blanc, Aart Lammers","doi":"10.1186/s12917-025-04902-w","DOIUrl":"10.1186/s12917-025-04902-w","url":null,"abstract":"<p><strong>Background: </strong>This study investigated the dose-dependent effects of a lactobacilli-based postbiotic (Post) on the transcriptional reprogramming of the chicken macrophage-like HD11 cell line when exposed to Escherichia coli lipopolysaccharide (LPS). First, the HD11cells were treated with 0, 3, 30 and 300 ng/mL LPS in combination with 0, 0.2, 0.4, 0.6 and 0.8% v/v Post. Nitric oxide (NO) production was quantified at 20 h incubation and the early transcriptome reprogramming was analysed in a subset of treatments at 5 h incubation.</p><p><strong>Results: </strong>Post increased NO production dose-dependently and an LPS-postbiotic interaction was present, with the cells eliciting a higher NO production in response to Post at 30 and 300 ng/ml LPS compared to the zero and 3 ng/ml LPS. To further understand this interaction, the early transcriptome reprogramming was investigated for treatments with 0, 3 and 300 ng/mL LPS and 0 and 0.8% v/v Post. A number of differentially expressed genes were identified and gene set enrichment analysis of KEGG pathways revealed that Post at 0 and 300 ng/mL LPS influenced similar inflammation-related pathways, until Post at 3 ng/mL LPS which had a minimal effect. Expression of transcription factors (TFs) and immune-related genes revealed differential effects induced by Post depending on LPS concentration which would have likely influenced the inflammatory response. Specifically, the only TFs affected by Post at 300 ng/ml LPS were STAT2, SMAD3 and IFR8, which all showed increased expression. The TFs consistently affected by Post at the zero and 3 ng/ml LPS increased and were JUN, ZFP36L2, SMAD1 and E2F3.</p><p><strong>Conclusion: </strong>Our results showed that Post had a pro-inflammatory effect, which was exacerbated in the presence of a 300 but not 3 ng/ml LPS. Furthermore, the dose of LPS affected the sensitivity of the cells to Post. Dose-response studies should be performed when investigating the effects of dietary compounds on inflammation in chicken macrophages.</p>","PeriodicalId":9041,"journal":{"name":"BMC Veterinary Research","volume":"21 1","pages":"479"},"PeriodicalIF":2.3,"publicationDate":"2025-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12278625/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144681946","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Weaning age impacts intestinal stabilization of jejunal intraepithelial T lymphocytes and mucosal microbiota in pigs.","authors":"Jayne E Wiarda, Hannah R Watkins, Melissa S Monson, Christopher L Anderson, Crystal L Loving","doi":"10.1186/s12917-025-04850-5","DOIUrl":"10.1186/s12917-025-04850-5","url":null,"abstract":"<p><p>Weaning in conventional pig production is a stressful event that involves abrupt dietary and environmental changes, and the post-weaning period is associated with increased incidence of disease and antibiotic use. As a result, there is a growing demand for non-antibiotic practices to enhance health during this phase of production. Current production systems wean piglets at a relatively young age, and it is unclear if age at weaning impacts shifts in intestinal immune populations, particularly intraepithelial T lymphocyte (T-IEL) populations, or bacterial communities, in a comparable timeframe and magnitude. T-IELs reside in the intestinal epithelium and play a role in intestinal integrity and defense. While later weaning may be an approach to minimize the negative impacts of weaning, the impact of age at weaning on T-IEL abundances and function is not clearly understood. Our results indicate pigs weaned at 18-21 days of age (doa; standard weaned, SW), which is consistent with production practices in the United States, had delayed shifts in T-IEL populations when compared to pigs weaned at 25-28 doa (late weaned, LW), which is consistent with practices in the European Union. Specifically, the abundance of induced T-IELs (CD8αβ⁺ αβ T cells) in the jejunum increased between 0 and 3 days post-weaning (dpw) for the LW group and stabilized, but shifts were delayed until between 3 and 7dpw in the SW group. Concomitant decreases in the abundance of natural T-IEL (γδ T cells) were detected. The structure of jejunal mucosal bacterial communities were comparable between SW and LW pigs at 0dpw, but the SW group had more pronounced shifts from 3 to 7dpw and 7dpw to 14dpw compared to minimal shift in LW group from 7 to 14dpw. Body weight between groups was comparable when adjusted for age. Overall, the bacterial communities of the jejunal mucosa were more stable after weaning in LW than SW pigs, and jejunal T-IEL abundances stabilized more rapidly in the LW group. As T-IELs play a key role in intestinal homeostasis and barrier integrity, the early differences in population abundance may be indicative of functional differences as well.</p>","PeriodicalId":9041,"journal":{"name":"BMC Veterinary Research","volume":"21 1","pages":"477"},"PeriodicalIF":2.3,"publicationDate":"2025-07-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12275383/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144667070","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}