Isolation and characterization of Pseudomonas phage HJ01 and its therapeutic efficacy in canine pyoderma.

IF 2.3 2区 农林科学 Q1 VETERINARY SCIENCES
Yixian Wei, Ziyong Li, Jianlong Lao, Jijie Huang, Hailin Chen, Jiajia Li, Yayu Deng, Xinyu Mao, Runwen Ma, Yuxing Wu, Yizhou Tan, Xun Li, Yangqing Lu, Shunjin Jiang, Xiaoye Wang
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Abstract

Background: Pseudomonas aeruginosa is a major pathogen in canine pyoderma, with increasing antibiotic resistance necessitating the development of alternative therapies. This study aimed to isolate and characterize a lytic phage targeting multidrug-resistant P. aeruginosa and evaluate its therapeutic efficacy in a canine pyoderma model. The key methods include isolation of phage HJ01 from wastewater, identification of genomic and biological characteristics, and subcutaneous injection of HJ01 in a canine pyoderma model induced by P. aeruginosa.

Results: The host strain GDPA-01 exhibited multidrug resistance, with a 75% resistance gene detection rate, and harbored virulence genes such as exoU and exoT. Phage HJ01, classified as Pakpunavirus, exhibited optimal activity at pH 6.0-8.0, thermal stability up to 50 °C, a latent period of 20 min, and a burst size of 52 PFU/cell. In vivo, compared with the controls, HJ01 significantly reduced pruritus, cytology, and skin lesion scores (p < 0.05). Histopathology confirmed that in dogs treated with HJ01, epidermal repair was accelerated and inflammation was reduced.

Conclusions: HJ01 emerges as a promising therapeutic candidate for managing multidrug-resistant P. aeruginosa infections in companion animals. This study pioneers the application of phage therapy in canine pyoderma, highlighting its potential to mitigate antibiotic overuse and address public health risks associated with zoonotic multidrug-resistant bacteria.

假单胞菌噬菌体HJ01的分离鉴定及其治疗犬脓皮病的疗效。
背景:铜绿假单胞菌是犬脓皮病的主要病原体,随着抗生素耐药性的增加,需要开发替代疗法。本研究旨在分离和鉴定一种针对耐多药铜绿假单胞菌的噬菌体,并在犬脓皮病模型中评价其治疗效果。关键方法包括从废水中分离噬菌体HJ01,鉴定其基因组和生物学特性,并皮下注射HJ01建立铜绿假单胞菌诱导的犬脓皮病模型。结果:宿主菌株GDPA-01表现出多药耐药,耐药基因检出率达75%,携带exoU、exoT等毒力基因。噬菌体HJ01属于Pakpunavirus,在pH 6.0 ~ 8.0时具有最佳活性,热稳定性可达50℃,潜伏期为20 min,爆发大小为52 PFU/cell。在体内,与对照组相比,HJ01显著降低了瘙痒、细胞学和皮肤损伤评分(p)。结论:HJ01是治疗伴侣动物多药耐药铜绿假单胞菌感染的有希望的治疗候选药物。本研究开创了噬菌体治疗犬脓皮病的应用,强调了其在减轻抗生素过度使用和解决与人畜共患多重耐药细菌相关的公共卫生风险方面的潜力。
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来源期刊
BMC Veterinary Research
BMC Veterinary Research VETERINARY SCIENCES-
CiteScore
4.80
自引率
3.80%
发文量
420
审稿时长
3-6 weeks
期刊介绍: BMC Veterinary Research is an open access, peer-reviewed journal that considers articles on all aspects of veterinary science and medicine, including the epidemiology, diagnosis, prevention and treatment of medical conditions of domestic, companion, farm and wild animals, as well as the biomedical processes that underlie their health.
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