{"title":"陕西省宠物戊型肝炎病毒血清学和分子学调查。","authors":"Yiyang Chen, Huanqing Lin, Shenhao Xu, Longzhi Nie, Yujia Tang, Xinru Li, DanBa Zhaxi, Chengwei Zhang, Qin Zhao, En-Min Zhou, Baoyuan Liu","doi":"10.1186/s12917-025-04898-3","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>Hepatitis E virus (HEV) is a zoonotic pathogen, and its infection is widespread in China. However, few studies were carried out on pets, especially in western China. Therefore, this study aimed to characterize the prevalence of HEV infection in pet dogs, cats, and rabbits. Serum samples from 177 pet dogs, 98 pet cats, and 56 pet rabbits were collected from a pet hospital located in Xi'an city of Shaanxi province, western China, to detect anti-HEV antibodies and HEV RNA.</p><p><strong>Results: </strong>The positivity rates of HEV IgG were 18.08% (32/177), 16.33% (16/98), and 10.71% (6/56) in pet dogs, cats, and rabbits, respectively. In addition, higher OD values of the anti-HEV IgM antibody were chosen for HEV detection, and the HEV RNA positivity rates were 0% (0/177), 0% (0/98), and 8.93% (5/56). Meanwhile, two different partial HEV ORF1 genes were obtained from 5 positive pet rabbit samples. These sequences shared 88.2% identity with each other and 48.6-98.2% identity with other HEVs. Phylogenetic analysis revealed that CN-SX-R1 and CN-SX-R2 (GenBank Nos. PP982770 and PP982771) detected in this study were closely related to genotype 3 HEV strains and belonged to rabbit HEVs.</p><p><strong>Conclusions: </strong>These results showed that HEV was prevalent in pet dogs, cats, and rabbits in Shaanxi, suggesting that necessary measures must be taken to prevent and control HEV infection in these species.</p>","PeriodicalId":9041,"journal":{"name":"BMC Veterinary Research","volume":"21 1","pages":"434"},"PeriodicalIF":2.3000,"publicationDate":"2025-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12225384/pdf/","citationCount":"0","resultStr":"{\"title\":\"Serological and molecular survey of hepatitis E virus in pets in shaanxi, China.\",\"authors\":\"Yiyang Chen, Huanqing Lin, Shenhao Xu, Longzhi Nie, Yujia Tang, Xinru Li, DanBa Zhaxi, Chengwei Zhang, Qin Zhao, En-Min Zhou, Baoyuan Liu\",\"doi\":\"10.1186/s12917-025-04898-3\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Background: </strong>Hepatitis E virus (HEV) is a zoonotic pathogen, and its infection is widespread in China. However, few studies were carried out on pets, especially in western China. Therefore, this study aimed to characterize the prevalence of HEV infection in pet dogs, cats, and rabbits. Serum samples from 177 pet dogs, 98 pet cats, and 56 pet rabbits were collected from a pet hospital located in Xi'an city of Shaanxi province, western China, to detect anti-HEV antibodies and HEV RNA.</p><p><strong>Results: </strong>The positivity rates of HEV IgG were 18.08% (32/177), 16.33% (16/98), and 10.71% (6/56) in pet dogs, cats, and rabbits, respectively. In addition, higher OD values of the anti-HEV IgM antibody were chosen for HEV detection, and the HEV RNA positivity rates were 0% (0/177), 0% (0/98), and 8.93% (5/56). Meanwhile, two different partial HEV ORF1 genes were obtained from 5 positive pet rabbit samples. These sequences shared 88.2% identity with each other and 48.6-98.2% identity with other HEVs. Phylogenetic analysis revealed that CN-SX-R1 and CN-SX-R2 (GenBank Nos. 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引用次数: 0
摘要
背景：戊型肝炎病毒（HEV）是一种人畜共患病原体，在中国广泛感染。然而，很少对宠物进行研究，特别是在中国西部。因此，本研究旨在描述宠物狗、猫和兔子中HEV感染的流行情况。从陕西省西安市某宠物医院采集177只宠物狗、98只宠物猫和56只宠物兔的血清，检测抗HEV抗体和HEV RNA。结果：宠物犬、猫和家兔的HEV IgG阳性率分别为18.08%（32/177）、16.33%（16/98）和10.71%（6/56）。此外，选择较高OD值的抗HEV IgM抗体检测HEV， HEV RNA阳性率分别为0%（0/177）、0%（0/98）和8.93%（5/56）。同时，从5份阳性家兔样品中获得了两种不同的HEV ORF1部分基因。这些序列之间的同源性为88.2%，与其他hev的同源性为48.6% ~ 98.2%。系统发育分析表明，本研究检测到的CN-SX-R1和CN-SX-R2 （GenBank编号PP982770和PP982771）与3型HEV株密切相关，属于兔HEV。结论：HEV在陕西省宠物犬、猫和家兔中流行，应采取必要的预防和控制措施。
Serological and molecular survey of hepatitis E virus in pets in shaanxi, China.
Background: Hepatitis E virus (HEV) is a zoonotic pathogen, and its infection is widespread in China. However, few studies were carried out on pets, especially in western China. Therefore, this study aimed to characterize the prevalence of HEV infection in pet dogs, cats, and rabbits. Serum samples from 177 pet dogs, 98 pet cats, and 56 pet rabbits were collected from a pet hospital located in Xi'an city of Shaanxi province, western China, to detect anti-HEV antibodies and HEV RNA.
Results: The positivity rates of HEV IgG were 18.08% (32/177), 16.33% (16/98), and 10.71% (6/56) in pet dogs, cats, and rabbits, respectively. In addition, higher OD values of the anti-HEV IgM antibody were chosen for HEV detection, and the HEV RNA positivity rates were 0% (0/177), 0% (0/98), and 8.93% (5/56). Meanwhile, two different partial HEV ORF1 genes were obtained from 5 positive pet rabbit samples. These sequences shared 88.2% identity with each other and 48.6-98.2% identity with other HEVs. Phylogenetic analysis revealed that CN-SX-R1 and CN-SX-R2 (GenBank Nos. PP982770 and PP982771) detected in this study were closely related to genotype 3 HEV strains and belonged to rabbit HEVs.
Conclusions: These results showed that HEV was prevalent in pet dogs, cats, and rabbits in Shaanxi, suggesting that necessary measures must be taken to prevent and control HEV infection in these species.
期刊介绍：
BMC Veterinary Research is an open access, peer-reviewed journal that considers articles on all aspects of veterinary science and medicine, including the epidemiology, diagnosis, prevention and treatment of medical conditions of domestic, companion, farm and wild animals, as well as the biomedical processes that underlie their health.
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