BMC Veterinary Research最新文献

{"title":"Metastasizing dysgerminoma in an inland bearded dragon (Pogona vitticeps).","authors":"Silvana Schmidt-Ukaj, Rene Brunthaler, Michaela Gumpenberger","doi":"10.1186/s12917-024-04368-2","DOIUrl":"10.1186/s12917-024-04368-2","url":null,"abstract":"<p><strong>Background: </strong>Malignant dysgerminomas are infrequently reported ovarian neoplasms in animals, especially in exotic pets (non-traditional companion animals [NTCAs]). In the few published case reports on reptilian species, examples are primarily postmortem without antemortem (clinical) assessment.</p><p><strong>Patient presentation: </strong>An adult, 13-year-old, spayed female inland bearded dragon (Pogona vitticeps) presented with lethargy, a right-sided head tilt, unilateral exophthalmos and ventrotemporal strabismus on the right eye. On examination, a palpable mass (approximately 3.5 cm in diameter) was detected within the mid coelomic cavity. Computed tomography revealed a retrobulbar swelling and lytic bone lesions affecting the right frontal bone and several vertebrae (T11, T13, and T14). Multiple nodules of soft tissue opacity were also detected within the lungs, liver, and coelomic fat bodies. Haematology revealed leukocytosis with heterophilia and toxic granulation of heterophils. On the basis of these results, differential diagnoses included disseminated abscesses, granulomas (e.g., due to mycobacteriosis) and neoplasms. The lizard was subsequently euthanized due to end-stage disease and a poor prognosis. Postmortem gross examination and histopathology revealed a primary ovarian dysgerminoma with evidence of widespread metastasis as well as localized tissue destruction affecting the right retrobulbar space and frontal bone, the spinal column, the lungs, the liver, and both coelomic fat bodies.</p><p><strong>Conclusions: </strong>This report describes a case of malignant dysgerminoma with widespread intraosseous and visceral metastases in a captive inland bearded dragon (Pogona vitticeps).</p>","PeriodicalId":9041,"journal":{"name":"BMC Veterinary Research","volume":"20 1","pages":"552"},"PeriodicalIF":2.3,"publicationDate":"2024-12-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11619615/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142778881","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The impact of stalking hunt season on long-term stress in big game.","authors":"Katarzyna Tajchman, Paweł Janiszewski, Patrycja Staniszewska, Vladimir Hanzal, Kornel Kasperek, Aneta Strachecka","doi":"10.1186/s12917-024-04416-x","DOIUrl":"10.1186/s12917-024-04416-x","url":null,"abstract":"<p><strong>Background: </strong>The frequent presence of hunters in hunting areas may have a negative impact on wild animals, which may disturb their welfare. Stressors may long affect individual animal species in different ways. Therefore, the study aimed to compare cortisol level in the hair of male mouflons, red deer, and wild boars harvested at the end of the stalking hunting season depending on the age and carcass mass. The cortisol level was determined using the DetectX^® Cortisol ELISA Kits.</p><p><strong>Results: </strong>The concentration of cortisol in the hair of mouflons was almost six times lower than that of red deer and wild boars. Carcass mass and age researched animals did not affect cortisol levels.</p><p><strong>Conclusions: </strong>Stalking hunts most likely do not impact the welfare of big game. There is probably habituation to long-term stress in the animals studied. Mouflons have the ability to respond effectively to specific stressors and are more resistant to long-term stress.</p>","PeriodicalId":9041,"journal":{"name":"BMC Veterinary Research","volume":"20 1","pages":"549"},"PeriodicalIF":2.3,"publicationDate":"2024-12-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11616240/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142778978","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Double-blinded, randomized tolerance study of a biologically enhanced Nanogel with endothelin-1 and bradykinin receptor antagonist peptides via intra-articular injection for osteoarthritis treatment in horses.","authors":"Antoinette Terlinden, Sandrine Jacquet, Seng Manivong, Aurélie Cullier, Frédéric Cassé, Florence Legendre, Araceli Ac Garcia, Gaëlle Roullin, Florina Moldovan, Pierre Sirois, Xavier Banquy, Philippe Galéra, Fabrice Audigié, Magali Demoor, Lélia Bertoni","doi":"10.1186/s12917-024-04352-w","DOIUrl":"10.1186/s12917-024-04352-w","url":null,"abstract":"<p><strong>Background: </strong>Osteoarthritis is a leading cause of pain and retirement in athletic horses. Hydro-expansive functionalized nanogels, acting as Drug Delivery Systems, constitute one of the current therapeutic prospects. These nanogels have the potential to combine mechanical benefits through polymers with the biological effect of prolonged release of bioactive molecules. The purpose of this double-blinded randomized tolerance study versus negative control was to evaluate the response of healthy joints to a single injection of the expected efficient dose (further referred to as the trial dose) and overdose of nanogels composed of chitosan and hyaluronic acid and featuring a type A endothelin receptor antagonist and a type B1 bradykinin receptor antagonist. The metacarpophalangeal joints of 8 healthy horses were randomly injected with 2.4 mL of functionalized nanogels and 2.4 mL of saline as control on the contralateral limb. Injections were repeated twice at one-week intervals, followed by injection of a triple dose of nanogel on week four. Clinical, ultrasonographic and synovial fluid cellular and biochemical follow-ups were performed up to three months following the first injection.</p><p><strong>Results: </strong>No change in general clinical parameters, lameness or sensitivity to passive flexion of the fetlocks was noted. Mild to moderate synovitis was noted on the day following injection in the treated group, with a significant difference (p < 0.05) compared to the control group. It spontaneously resolved on day 3 following the injections and did not increase with repeated injections. Similar effects were noted after injection of the triple dose but lasted for a week. Synovial fluid markers of inflammation also showed a transient significant increase in the treated group one week after each injection, but no differences were detected at the end of the study.</p><p><strong>Conclusions: </strong>Injections of the expected therapeutic dose of functionalized nanogel in healthy joints induced a mild transient inflammatory response in the joint. Three injections of the trial dose at one-week intervals and injection of thrice the trial dose induce a mildly greater inflammation without harmful effects on joints. Functionalized nanogels are well tolerated prospects for the treatment of osteoarthritis in horses. Their beneficial effects on arthritic joints have yet to be evaluated to determine their therapeutic potential.</p>","PeriodicalId":9041,"journal":{"name":"BMC Veterinary Research","volume":"20 1","pages":"547"},"PeriodicalIF":2.3,"publicationDate":"2024-12-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11616385/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142778854","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Influence of volatile anaesthetics on haematology and clinical chemistry in ferrets.","authors":"Marie-Luise Schröder, Aline Reitmeier","doi":"10.1186/s12917-024-04407-y","DOIUrl":"10.1186/s12917-024-04407-y","url":null,"abstract":"<p><strong>Background: </strong>During our years of working with ferrets in our laboratory animal facility, we observed that in several healthy young female ferrets, signs of anaemia could be detected during the entry examination although none of the ferrets expressed any clinical symptoms at the time of blood withdrawal. We aimed to compare the influence of inhalation anaesthesia with isoflurane and sevoflurane to restrained, awake ferrets on several blood parameters. After arrival at our facility all ferrets received a hormone chip to subdue oestrus. Routine blood withdrawal was conducted followed by routine blood work including haematology, clinical chemistry and electrolytes. Since the size of the cannula for implementation of the hormone chip is relatively large and the insertion probably quite painful the procedure was always performed during a short inhalation anaesthesia with isoflurane or sevoflurane. In ferrets showing anaemia (haemetocrit below 0.37 l/L, haemoglobin below 11 g/dL), we performed a control blood work (venous blood) the following week. In order to rule out an effect of inhalation anaesthesia on the laboratory results, the blood withdrawal was performed in restraint and awake ferrets without anaesthesia. The study was performed as randomized controlled crossover design.</p><p><strong>Results: </strong>Thirty ferrets were enrolled, and divided in three groups. Comparison of the three methods (isoflurane, sevoflurane or restraint and awake without anaesthesia) of blood withdrawal showed statistically significant (p < 0.05) differences in most haematological parameters (e.g. red blood cell count, haematocrit), clinical chemistry parameters (e.g. total protein, albumin, alkaline phosphatase) and electrolytes (e.g. sodium, chloride and potassium). Restraint ferrets without anaesthesia showed no signs of anaemia. In anaesthetized ferrets, reticulocytes were about four to six times lower compared to ferrets anaesthetized with isoflurane or sevoflurane.</p><p><strong>Conclusions: </strong>The results of the study suggest that inhalation anesthesia has a significant effect on hematological and biochemical parameters in ferrets. In particular, the detection of anemia in an animal undergoing inhalation anesthesia needs to be contextualized in a clinical setting and in research context.</p>","PeriodicalId":9041,"journal":{"name":"BMC Veterinary Research","volume":"20 1","pages":"551"},"PeriodicalIF":2.3,"publicationDate":"2024-12-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11616128/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142778858","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Paratuberculosis in South American camelids: two independent cases in alpacas in Germany.","authors":"Heike Köhler, Jana Müller, Elena Kloß, Petra Möbius, Stefanie A Barth, Marlene Sickinger, Nicole Gies, Carsten Heydel, Martin Peters","doi":"10.1186/s12917-024-04414-z","DOIUrl":"10.1186/s12917-024-04414-z","url":null,"abstract":"<p><strong>Background: </strong>Paratuberculosis, caused by Mycobacterium avium subspecies paratuberculosis (MAP), is a chronic granulomatous enteritis that affects domestic and wild ruminants and camelids. The disease has rarely been reported in alpacas in Germany. This publication describes epidemiologically independent cases of paratuberculosis in two alpacas in Germany.</p><p><strong>Case presentation: </strong>Two alpacas, a 26-year-old female zoo animal (case 1) and a 2.5-year-old breeding stallion from a private owner (case 2), presented with progressive emaciation, leading to death (case 2) or euthanasia (case 1) because of deteriorating general condition. In both cases typical granulomatous lesions in the intestinal mucosa and mesenteric lymph nodes were found. In case 2, other lymph nodes were severely enlarged and MAP was detected in the mandibular lymph node, lung, and liver by qPCR. The MAP isolates differed between the alpacas, with two distinct phylogenetic clades (Clade 1 and 8) within Subgroup A of the MAP-C type group and two distinct INMV profiles (INMV 2 and 1) found. These genotypes have been identified in cattle and goats in different regions in Germany. The genotype isolated from case 1 has been detected in goats from the zoo since 2011, indicating transmission between these species.</p><p><strong>Conclusions: </strong>MAP can cause severe clinical disease in alpacas of variable age and under different husbandry conditions. Therefore, paratuberculosis should be considered for differential diagnosis in alpacas with emaciation and poor general condition. Although not definitely shown, cross-species infection between ruminant species and camelids is exceedingly likely.</p>","PeriodicalId":9041,"journal":{"name":"BMC Veterinary Research","volume":"20 1","pages":"550"},"PeriodicalIF":2.3,"publicationDate":"2024-12-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11616164/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142778964","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A quantitative survey on exposures, knowledge, attitudes, and practices (KAP) related to swine influenza among villagers in different regions of Thailand.","authors":"Kanokwan Suwannarong, Kannika Thammasutti, Thanomsin Ponlap, Phitsanuruk Kanthawee, Chutarat Saengkul, Paisit Boonyakawee, Rungsimun Pothita, Darunee Phosri, Supaporn Anuragudom, Suthutta Changtes, Alongkorn Amonsin","doi":"10.1186/s12917-024-04406-z","DOIUrl":"10.1186/s12917-024-04406-z","url":null,"abstract":"<p><p>This analytical cross-sectional study aimed to determine factors influencing knowledge, attitudes, and practices (KAP) toward the swine influenza virus (SIV) among pig farm owners, workers, and villagers in selected provinces of Thailand. This study was carried out from February to December 2022 in pig farms and villages across the provinces. A structured and standardized quantitative questionnaire was utilized to collect data on socio-demographic variables and KAP related to SIV from 215 participants. The quantitative data was analyzed in two steps using R software. The study findings showed that the average knowledge score on SIV was 6.92 out of 12, indicating moderate understanding of SIV in study participants. Attitudes towards SIV were generally positive, with an average score of 3.74 out of 5. Practices for SIV prevention averaged a score of 3.63 out of 5. Key factors associated with better SIV knowledge included higher education levels, higher monthly income, and direct involvement in vaccination processes. Moreover, those with higher education, employment on pig farms, and prior vaccination experience showed more positive attitudes towards SIV. Occupations related to pig farming and the availability of vaccination services significantly influenced SIV prevention practices. The findings highlighted that improved education and stronger connections with healthcare professionals and the pig farming sector may significantly enhance KAP regarding SIV among targeted populations. In contrast, participants with limited exposure to health services or pig farming activities, such as nearby villagers, require specialized educational interventions. This study recommends that local health authorities should develop and implement communication strategies and interventions focused on educating pig farm owners, managers, and villagers about SIV to mitigate the risks associated with SIV and other zoonotic diseases.</p>","PeriodicalId":9041,"journal":{"name":"BMC Veterinary Research","volume":"20 1","pages":"548"},"PeriodicalIF":2.3,"publicationDate":"2024-12-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11616355/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142778817","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Live attenuated goatpox vaccination in pregnant Murcia-Granada goats: dosage implications and outcomes.","authors":"Hossein Esmaeili, Mohammadreza Ghorani, Seyed Mehdi Joghataei, Sergio Villanueva-Saz, Delia Lacasta","doi":"10.1186/s12917-024-04395-z","DOIUrl":"10.1186/s12917-024-04395-z","url":null,"abstract":"<p><strong>Background: </strong>Infectious diseases, particularly the Goatpox virus (GTPV) from the Poxviridae family, significantly impact livestock health and agricultural economies, especially in developing regions. Recent GTPV outbreaks in previously eradicated areas underscore the need for effective control measures, with vaccination being the most reliable strategy. This study investigates the effects of administering standard and double doses of live attenuated goatpox vaccine in pregnant Murcia-Granada goats, a non-native breed in Iran, to determine optimal vaccination protocols.</p><p><strong>Results: </strong>In 2018, 400 healthy and pregnant Murcia Granada goats imported from Spain were divided into groups of 200 and vaccinated with either a standard dose (0.5 ml) or a double dose (single 0.9 ml injection) of live attenuated goatpox vaccine. Post-vaccination, the goats were monitored daily for clinical signs of infection, with samples collected for PCR analysis to detect the presence of GTPV strains. In group A, which received the standard vaccine dose, no abortions or vaccine-related side effects were observed, and body temperatures remained normal. In group B, administered a double dose, 37% of the goats experienced abortions, displaying signs of GTPV infection, such as skin lesions (pox lesions) and increased body temperatures. Molecular analysis confirmed the vaccine strain of GTPV as the infection source, ruling out external contamination. Statistical analysis showed no significant differences in abortion rates concerning gestational age or t he age of the pregnant goats.</p><p><strong>Conclusion: </strong>The study highlights the importance of adhering to standard vaccine dosages in pregnant Murcia Granada goats to prevent adverse outcomes like abortions. This study emphasizes the necessity to review and revise vaccination protocols tailored to specific breeds and varying maintenance conditions, including pregnancy and outbreak scenarios. These findings stress the necessity for cautious and tailored vaccination strategies to ensure the safety and efficacy of vaccines in different goat breeds.</p>","PeriodicalId":9041,"journal":{"name":"BMC Veterinary Research","volume":"20 1","pages":"544"},"PeriodicalIF":2.3,"publicationDate":"2024-12-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11610293/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142766137","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Differential modulation of inflammatory cytokines by recombinant IL-10 in IL-1β and TNF-α ̶ stimulated equine chondrocytes and synoviocytes: impact of washing and timing on cytokine responses.","authors":"Hoda A Elkhenany, Renata L Linardi, Kyla F Ortved","doi":"10.1186/s12917-024-04403-2","DOIUrl":"10.1186/s12917-024-04403-2","url":null,"abstract":"<p><p>Osteoarthritis (OA) remains a challenging joint disorder necessitating effective anti-inflammatory interventions. In this study, our primary objective was to establish an in vitro protocol that replicates the clinical investigation of anti-inflammatory drugs intended for OA management. Focusing on recombinant IL-10 (r.IL-10) as a potential anti-inflammatory treatment, we designed and implemented two distinct protocols to evaluate the efficacy of r.IL-10 in modulating chondrocyte and synoviocyte inflammation.The experimental design involved sequential stimulation with IL-1β and TNF-α for 24 h, followed by washing (model 1) or not washing (model 2) the cells before r.IL-10 treatment. Samples were collected after 6-24 h of treatment. Cellular responses were evaluated by quantifying gene expression and synthesis of key inflammatory cytokines and proteases.The expression and synthesis of inflammatory cytokines and proteases was significantly affected by washing and treatment time. The expression of IL-1β, TNF-α, IL-8, MMP-13, and ADAMTS5 were effectively reduced in r.IL-10-treated chondrocytes and synoviocytes in model 2 after 24 h, particularly at concentrations of 10 and 20 ng/mL. r.IL-10 treatment significantly increased IL-6 gene expression in chondrocytes at all time points. However, in synoviocytes, IL-6 expression was significantly lower in model 2 after 24 h of r.IL-10 treatment. r.IL-10 treatment significantly decreased IL-1β and TNF-α content in synoviocyte supernatants, particularly in model 2 at concentrations of 10 and 20 ng/mL after 6 and 24 h. r.IL-10 treatment in chondrocytes led to a significant decrease in IL-1β supernatant concentrations in model 2 after 24 h only.This study demonstrated that r.IL-10 treatment effectively reduces key inflammatory markers and matrix metalloproteinase activity in both chondrocytes and synoviocytes, particularly in model 2 where cells were not washed prior to treatment. These findings highlight r.IL-10's potential as a robust anti-inflammatory agent for OA management and suggest its critical role in developing effective therapeutic strategies for OA.</p>","PeriodicalId":9041,"journal":{"name":"BMC Veterinary Research","volume":"20 1","pages":"546"},"PeriodicalIF":2.3,"publicationDate":"2024-12-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11610082/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142766135","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Subcutaneous inoculation of Escherichia coli in broiler chickens causes cellulitis and elicits innate and specific immune responses.","authors":"Liv Jonare, Eva Wattrang, Emma Östlund, Helena Wall, Magdalena Jacobson, Désirée S Jansson","doi":"10.1186/s12917-024-04392-2","DOIUrl":"10.1186/s12917-024-04392-2","url":null,"abstract":"<p><strong>Background: </strong>Cellulitis caused by Escherichia coli is a common cause of condemnation of broiler chickens at slaughter worldwide and is associated with economic losses and a possible negative impact on animal welfare. The study objective was to monitor clinical signs and immune responses after subcutaneous E. coli inoculation (1.1-1.8 × 10⁷ CFU), aiming to induce cellulitis. Three groups of broiler chickens (n = 15/group) were inoculated with well-characterized E. coli strains (group A: ECA18 O24:H4/ST117 and group B: ECB11 O153:H9/ST38) or with saline (control) at 22 days-of-age. Clinical signs of disease, body weight and immune parameters were monitored until euthanasia 12-14 days after inoculation followed by post-mortem examination.</p><p><strong>Results: </strong>The daily weight gain of the inoculated chickens was significantly lower one day after inoculation compared to the controls. Seven (23%) of the inoculated chickens displayed clinical signs: ruffled feathers, mild weakness, open-beak breathing and/or reluctance to stand, of which two birds were euthanized and one bird died. Five chickens in group B were observed with bacteraemia, which lasted up to three days after inoculation for two chickens. A transient increase in chicken mannose receptor MRC1L-B expression on circulating monocytes was observed one day after inoculation in both E. coli inoculated groups, with a more pronounced increase in group B. On day 7 after inoculation, the in vitro adherence of heterophils, monocytes and thrombocytes to the inoculated strain was increased in group B. Antibody titers to the inoculation strains were increased in some chickens in both groups on days 7 and 14 after inoculation, with the highest titers in group B. Seven (47%) and 13 (87%) of the chickens in group A and B, respectively, were diagnosed with cellulitis at post-mortem examination. In most birds, lesions consisted of plaque-like material embedded in the subcutaneous tissue of the abdominal wall.</p><p><strong>Conclusions: </strong>Inoculation of E. coli caused cellulitis and prompted a rapid activation/redistribution of circulating monocytes followed by antibody production. The responses were most pronounced in chickens inoculated with E. coli strain ECB11, presumably because of a higher virulence.</p>","PeriodicalId":9041,"journal":{"name":"BMC Veterinary Research","volume":"20 1","pages":"545"},"PeriodicalIF":2.3,"publicationDate":"2024-12-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11610265/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142766138","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Seroprevalence and serotype distribution of foot and mouth disease virus and associated risk factors in cattle across various export livestock sourcing districts of Bale Zone, Ethiopia.","authors":"Daniel Gizaw, Demessa Negessu, Ayenalem Fentie, Ayelech Muluneh, Hagos Asgedom, Chala Guyassa, Hagos Ashenafi, Wudu T Jemberu, Mengistu Legesse, Tesfu Kassa","doi":"10.1186/s12917-024-04382-4","DOIUrl":"10.1186/s12917-024-04382-4","url":null,"abstract":"<p><strong>Background: </strong>Foot and mouth disease (FMD) is a contagious and economically important viral disease affecting cloven-hoofed animals. A cross-sectional study was conducted from January to March 2022 in selected districts of Bale zone, Ethiopia to determine the seroprevalence and serotypes of FMD virus circulating in cattle. Blood samples were collected from cattle and tested for antibodies against non-structural proteins (NSP) of FMD virus using a 3ABC enzyme-linked immunosorbent assay (ELISA). Positive samples were further tested for serotype-specific antibodies using solid phase competitive ELISA (SPCE). Descriptive statistics, both univariable and multivariable logistic regression were used to analyse FMD serostatus and associated risk factors.</p><p><strong>Results: </strong>A total of 962 cattle sera were tested and 200 samples, 20.8% (95% CI: 18.3-23.5) were positive for antibodies against NSP of the FMD virus. The highest seroprevalence was observed in the Seweyna district (35.2%, 95%CI: 26.8-44.7) and the lowest in the Dinsho district (13.0%, 95% CI: 9.5-17.5). The seroprevalence of FMD virus antibody was significantly associated with district (P < 0.05). The seroprevalence among different age groups was statistically significant (P < 0.05). The odds of FMD infection for males was 0.76 (95% CI: 0.534-1.082) compared to females, indicating that male cattle have a lower risk for FMD infection. Out of 200 samples tested for serotype O, A, SAT 1, and SAT 2, 85(43%) were found to have serotype O, 59(30%) serotype A, 142(71%) serotype SAT 1, and 75 (38%) serotype SAT2. Furthermore, multiple FMD serotypes were observed in 15-40% (30-80) of animals tested.</p><p><strong>Conclusions: </strong>Serotype-specific antibodies against the FMD virus indicate the occurrence and distribution of serotypes O, A, SAT1, and SAT2 in cattle across various districts of the Bale zone in Ethiopia. These findings also highlight the importance of continuously monitoring the seroprevalence of FMD virus serotypes circulation in export livestock sourcing areas. The results indicated that four FMD virus serotypes were distributed across the studied districts. This study supports the inclusion of all four FMD serotypes in vaccine production.</p>","PeriodicalId":9041,"journal":{"name":"BMC Veterinary Research","volume":"20 1","pages":"543"},"PeriodicalIF":2.3,"publicationDate":"2024-11-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11607933/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142754671","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}