BMC Veterinary Research最新文献

{"title":"Hepatic encephalopathy associated with extensive hepatic diaphragmatic herniation in an adult horse.","authors":"Markyson Tavares Linhares, Isabelle Vieira de Sousa, Lilian Rayanne de Castro Eloy, José Machado de Oliveira Neto, Luis Jhordy Alfaro Quillas, Daniel Guimarães Ubiali, Lucas Rannier Ribeiro Antonino Carvalho, Ricardo Barbosa Lucena","doi":"10.1186/s12917-026-05752-w","DOIUrl":"10.1186/s12917-026-05752-w","url":null,"abstract":"<p><strong>Background: </strong>Diaphragmatic hernia in horses is a rare condition, most commonly presenting with signs of colic and, less frequently, respiratory compromise. Hepatic herniation through a diaphragmatic defect with secondary hepatic failure and encephalopathy has rarely been documented in horses.</p><p><strong>Case presentation: </strong>A 5-year-old Mangalarga Marchador stallion was evaluated for progressive neurological deterioration, including obtundation, head pressing, ataxia, and circling. Serum biochemistry revealed severe hepatobiliary dysfunction, characterised by increased gamma-glutamyl transferase and alkaline phosphatase activities, hyperbilirubinaemia and hypertriglyceridaemia. The horse died within 12 h of admission. Post-mortem examination revealed a chronic pleuroperitoneal diaphragmatic defect with herniation of the left lateral, left medial, and quadrate hepatic lobes into the thoracic cavity. The herniated portion showed severe atrophy, haemorrhagic necrosis, cholestasis, and ductular proliferation, while the non-herniated hepatic mass exhibited bridging fibrosis and nodular regenerative remodelling. Histopathological examination of the brain confirmed the presence of Alzheimer type II astrocytes in the cerebral cortical grey matter, consistent with hepatic encephalopathy.</p><p><strong>Conclusions: </strong>This case documents an unusual manifestation of equine diaphragmatic hernia in which extensive chronic hepatic incarceration resulted in severe hepatobiliary dysfunction and hepatic encephalopathy. The findings suggest that diaphragmatic hernia with hepatic involvement should be considered in the differential diagnosis of horses presenting with severe hepatopathy and neurological signs compatible with hepatic encephalopathy, particularly when classical signs of colic are absent.</p>","PeriodicalId":9041,"journal":{"name":"BMC Veterinary Research","volume":"22 1","pages":""},"PeriodicalIF":3.1,"publicationDate":"2026-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13428427/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148652843","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction: Application of XGBoost and Random Forest algorithms for body weight prediction in Blackbelly sheep using biometric measurements.","authors":"Miguel Ángel Gastelum Delgado, Cem Tırınk, Rosa Inés Parra-Cortés, Ignacio Vázquez Martínez, Armando Gomez-Vazquez, Aldenamar Cruz-Hernandez, Enrique Camacho-Pérez, Dany Alejandro Dzib-Cauich, Hasan Önder, Uğur Şen, Kadyrbai Chekirov, Yüksel Aksoy, Hilal Tozlu Çelik, Olatunbosun Odu, Alfonso J Chay-Canul","doi":"10.1186/s12917-026-05748-6","DOIUrl":"10.1186/s12917-026-05748-6","url":null,"abstract":"","PeriodicalId":9041,"journal":{"name":"BMC Veterinary Research","volume":"22 1","pages":""},"PeriodicalIF":3.1,"publicationDate":"2026-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13421091/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148618062","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Longitudinal changes in selected biochemical, hematological, and vital parameters in Gurcu goat kids: effects of age, sex, and birth type.","authors":"Yusuf Umut Bati, Mert Sezer, Enes Akyüz, Mushap Kuru","doi":"10.1186/s12917-026-05754-8","DOIUrl":"https://doi.org/10.1186/s12917-026-05754-8","url":null,"abstract":"<p><strong>Background: </strong>Biochemical, hematological, and vital parameters are important indicators of health status, growth, and physiological adaptation in young ruminants. However, breed-specific longitudinal data for Gurcu goat kids raised under traditional management conditions are limited. The primary objective of this study was to longitudinally evaluate age-related changes in selected biochemical, hematological, and vital parameters in healthy Gurcu goat kids, while sex and birth type were assessed as secondary modifying factors during postnatal growth.</p><p><strong>Methods: </strong>Twenty clinically healthy Gurcu goat kids, including 10 males and 10 females, were followed from 1 to 11 months of age. The study population included 10 single-born and 10 twin-born goat kids, with five animals in each combined sex-by-birth type subgroup. Blood samples and vital measurements were obtained at six sampling points: 1, 3, 5, 7, 9, and 11 months of age. Selected biochemical, hematological, and vital parameters were evaluated longitudinally according to age, sex, birth type, and combined sex-by-birth type subgroups.</p><p><strong>Results: </strong>Age-related variation was the predominant finding across the evaluated parameter groups. Time significantly affected most biochemical variables, most evaluated hematological parameters, and all three vital parameters, indicating marked physiological variation during postnatal growth. Representative time-related effects were observed for RT (P < 0.001, η_p² = 0.45), PR (P < 0.001, η_p² = 0.62), and RR (P < 0.001, η_p² = 0.44). In contrast, sex- and birth type-related differences were more limited and were detected only in selected parameters at specific sampling months. Significant group × time interactions were observed in selected biochemical parameters, including Ca (P = 0.002, η_p² = 0.34), Phos (P < 0.001, η_p² = 0.41), Crea (P < 0.001, η_p² = 0.43), AST (P = 0.03, η_p² = 0.27), and GGT (P < 0.001, η_p² = 0.51). These findings show that subgroup differences were stage-dependent rather than constant throughout the postnatal growth period.</p><p><strong>Conclusions: </strong>The main contribution of this study is the provision of breed-specific longitudinal baseline data for healthy Gurcu goat kids raised under traditional management conditions. Age should be considered the primary factor when interpreting biochemical, hematological, and vital parameters in growing Gurcu goat kids, whereas sex and birth type should be regarded as additional, parameter-specific modifiers. Because of the relatively small sample size and single-farm study design, further studies with larger populations raised under different environmental and management conditions are needed to confirm and expand these findings.</p>","PeriodicalId":9041,"journal":{"name":"BMC Veterinary Research","volume":"22 1","pages":""},"PeriodicalIF":3.1,"publicationDate":"2026-07-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13637090/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149103815","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Livestock vaccination during humanitarian crises in Sub-Saharan Africa: a scoping review of evidence of impact.","authors":"Buke Yussuf Wako, Theodore J D Knight-Jones, Christine Jost, Uswa Jafary, Chris Daborn, Shauna Richards","doi":"10.1186/s12917-026-05744-w","DOIUrl":"https://doi.org/10.1186/s12917-026-05744-w","url":null,"abstract":"<p><strong>Background: </strong>In Sub-Saharan Africa (SSA), livestock are central to livelihoods and food security for hundreds of millions of people, yet are acutely vulnerable during humanitarian crises including drought, conflict, and disease outbreaks. Mass vaccination is routinely deployed as an emergency livestock intervention, but evidence of its actual impact on herd survival, livelihoods, and human nutrition remains scarce and methodologically weak.</p><p><strong>Methods: </strong>This scoping review synthesized available evidence on livestock vaccination during humanitarian crises in SSA, drawing on 57 sources identified through structured database searches supplemented by grey literature and expert workshop insights.</p><p><strong>Results: </strong>Of these, only 20 directly addressed vaccination in crisis or disaster-response contexts, nine reported direct measures of impact such as mortality reduction, herd recovery, or economic outcomes and eleven comprising of guidance documents or programme reports without outcome measurement. Most relied on coverage figures, programme reports, or operational guidance rather than rigorous outcome evaluation. Findings are labelled according to their context of origin: crisis setting (CS), non-crisis setting (NCS), or programme guidance (PG). Three studies from Kenya and Ethiopia reported reduced livestock mortality, while one study from South Sudan reported a positive benefit-cost ratio in a crisis context with vaccination associated with reduced livestock mortality and positive economic returns, particularly when implemented early and integrated with feed, water, and veterinary support. Key barriers to effectiveness included cold chain failures, delayed delivery, antigenic mismatch, weak coordination, and inequitable access, with women consistently less likely to benefit from vaccination programmes.</p><p><strong>Conclusions: </strong>Based on this review, we recommend: (1) shifting evaluation frameworks from coverage outputs to measurable livestock and livelihood outcomes; (2) investing in preparedness and pre-crisis vaccination before animals are debilitated; (3) adopting thermostable vaccine formulations for remote and crisis settings; (4) systematically integrating gender equity into programme design and monitoring; and (5) prioritizing rigorous crisis-specific evaluation to strengthen the evidence base. Evaluation of crisis-response livestock vaccination remains methodologically weak and further rigorous evaluation is urgently needed.</p>","PeriodicalId":9041,"journal":{"name":"BMC Veterinary Research","volume":"22 1","pages":""},"PeriodicalIF":3.1,"publicationDate":"2026-07-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149242879","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Isolation of feline-derived scFvs against the VP1-CDE region of feline calicivirus from a phage display library and characterization of their antigen-binding and antiviral potential in vitro.","authors":"Yanquan Wei, Hongmei Wang, Zhen Han, Huitian Gou, Dongdong Sun, Yihan Liu","doi":"10.1186/s12917-026-05702-6","DOIUrl":"10.1186/s12917-026-05702-6","url":null,"abstract":"<p><strong>Background: </strong>Feline calicivirus (FCV) is a significant causative pathogen of upper respiratory tract disease and virulent systemic infection in feline populations, yet specific targeted antiviral agents for FCV infection remain unavailable.</p><p><strong>Methods: </strong>A feline-derived single-chain variable fragment (scFv) phage display library targeting the VP1-CDE neutralizing region of FCV was constructed using peripheral blood mononuclear cells isolated from cats immunized with recombinant VP1-CDE protein. Positive scFv clones were screened via three rounds of affinity panning and phage ELISA. The scFv-Fc fusion proteins were expressed in eukaryotic cells, and their antigen-binding properties and antiviral potential were characterized through Western blotting, bio-layer interferometry, immunofluorescence assay and viral neutralization assay.</p><p><strong>Results: </strong>The constructed library reached a size of 2.1 × 10⁸ independent phages with favorable sequence diversity. Five unique scFv clones, designated 3A5, 3B8, 3H5, 2H5, and 2I5, were identified. All five clones could specifically bind to both recombinant VP1-CDE protein and FCV-infected F81 feline kidney cells. Viral neutralization assays demonstrated that scFv-Fc-3A5 exhibited the most potent neutralizing activity against the genogroup II FCV LZ2023 isolate (IC₅₀= 1.862 µg/mL), scFv-Fc-2H5 showed weak neutralization (IC₅₀= 6.168 µg/mL), and the remaining three clones had no appreciable neutralizing capacity.</p><p><strong>Conclusions: </strong>scFv-Fc-3A5 and scFv-Fc-2H5 are promising candidate molecules for the development of diagnostic reagents and antiviral therapeutics targeting genogroup II FCV.</p>","PeriodicalId":9041,"journal":{"name":"BMC Veterinary Research","volume":"22 1","pages":""},"PeriodicalIF":3.1,"publicationDate":"2026-07-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13560130/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148918379","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multiscale epidemiology of gastrointestinal nematode infections in small ruminants: field evidence from Inner Mongolia and a national meta-analysis in China.","authors":"Xiaoqing Jia, Wei Wei, Zhihong Zhang, Lei Zhang, Ling Bai, Rui Shi, Yuan Ma, Rui Wang","doi":"10.1186/s12917-026-05738-8","DOIUrl":"10.1186/s12917-026-05738-8","url":null,"abstract":"<p><strong>Background: </strong>Gastrointestinal nematodes (GINs) are among the most important parasites constraining small-ruminant production. However, epidemiological evidence in China remains fragmented because existing studies differ substantially in geographic coverage, host species, sampling periods, diagnostic approaches, and ecological settings.</p><p><strong>Methods: </strong>We applied a multiscale framework integrating a regional survey in Inner Mongolia with a national systematic review and meta-analysis. In the regional survey, 2,084 fecal samples were collected from sheep aged 6-12 months in Ulanqab, Alxa, and Ordos from August 2020 to September 2021 and examined using a modified McMaster method. Detected eggs were classified into broad morphologic categories. For the national analysis, six databases were searched following PRISMA guidelines, and eligible cross-sectional studies were synthesized using random-effects models.</p><p><strong>Results: </strong>The regional survey showed an overall GIN prevalence of 28.8% (601/2,084), with marked spatial variation among Ordos (74.0%), Ulanqab (53.2%), and Alxa (11.9%). Strongyle-type and Nematodirus-type eggs were detected in 25.3% and 17.5% of samples, respectively, with co-positivity in 14.0%. Nationally, 50 studies comprising 53,955 small ruminants were included. The random-effects pooled prevalence was 71% (95% CI: 64%-78%), with extreme heterogeneity (I² = 100%). Marshallagia and Haemonchus showed relatively high pooled estimates among reported taxa, while subgroup differences by host species, age, and feeding system were not statistically significant.</p><p><strong>Conclusions: </strong>The findings demonstrate marked local variation in GIN prevalence in the regional survey and substantial descriptive spatial variation across the provincial-level estimates included in the national meta-analysis. The regional microscopy-based survey supports broad egg-category inference but not reliable genus- or species-level identification of non-Nematodirus strongylid nematodes. These results support geographically targeted surveillance and locally adapted parasite-control strategies, while national pooled and subgroup estimates should be interpreted cautiously because of marked heterogeneity.</p>","PeriodicalId":9041,"journal":{"name":"BMC Veterinary Research","volume":"22 1","pages":""},"PeriodicalIF":3.1,"publicationDate":"2026-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13685266/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149227246","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Combined cold atmospheric plasma and photobiomodulation is associated with improved early macroscopic and ultrasonographic wound-healing parameters after unilateral mastectomy in female dogs: an exploratory randomized intra-individual controlled clinical study.","authors":"Guilherme Ramon Vieira da Silva, Márcio Santos de Oliveira Filho, Samuel Ferreira de França Silvestre, Gabrielle Arruda Carneiro, Rivaldo Bruno Medeiros de Lucena, Clarisse Maria Rodrigues Costa, Pedro Lucas Diogo Facundo, Fernando Lucas Costa Silva, João Marcelo Azevedo de Paula Antunes, Larissa de Castro Demoner, Valéria Veras de Paula, Airton Torres Carvalho, Carlos Augusto Galvão Barboza, Clodomiro Alves Júnior, Moacir Franco de Oliveira, Marcela Maria de Almeida Amorim, Carlos Eduardo Bezerra de Moura","doi":"10.1186/s12917-026-05746-8","DOIUrl":"10.1186/s12917-026-05746-8","url":null,"abstract":"<p><strong>Background: </strong>Postmastectomy complications remain relevant in veterinary surgery. Although cold atmospheric plasma (CAP) and photobiomodulation (PBM) may improve tissue repair, evidence on their combined use is limited. This exploratory pilot study evaluated CAP-PBM therapy in nine female dogs undergoing unilateral mastectomy.</p><p><strong>Methods: </strong>A prospective, randomized, blinded, intra-individual controlled clinical study was conducted in nine female dogs with mammary neoplasia undergoing clinically indicated unilateral mastectomy. In each dog, one half of the surgical wound was randomly assigned to receive sequential CAP and PBM therapy, while the contralateral half remained untreated and served as an intra-individual control. Evaluations were performed on postoperative days 3, 7, and 14 (D3, D7, and D14) using macroscopic scoring systems, ultrasonography, and infrared thermography; histomorphometric analysis was performed only on D7.</p><p><strong>Results: </strong>Treated regions showed significantly lower Modified Hollander Scar Scale (p < 0.001) and Modified Draize Scoring System (p = 0.001) scores. Ultrasonographic assessment demonstrated lower semiquantitative scores (p = 0.029) and reduced subcutaneous thickness (p = 0.034), especially during the early postoperative period. Thermographic findings did not differ significantly between groups. Histological analysis revealed numerically greater re-epithelialization in treated areas, although without statistical significance (p > 0.05).</p><p><strong>Conclusions: </strong>In this exploratory pilot study, combined CAP + PBM therapy was associated with improved early macroscopic and ultrasonographic healing parameters after mastectomy in female dogs. These preliminary findings support further investigation of CAP + PBM as a promising non-invasive adjuvant strategy for postoperative wound management, pending confirmation in larger, adequately powered randomized studies.</p>","PeriodicalId":9041,"journal":{"name":"BMC Veterinary Research","volume":"22 1","pages":""},"PeriodicalIF":3.1,"publicationDate":"2026-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13685181/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149236757","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Atypical case of an orf virus infection in sheep.","authors":"Anna Segna, Isaac David Portobanco Villalobos, Martin Ganter, Martin Ludlow, Wolfgang Baumgärtner, Katharina Manuela Gregor","doi":"10.1186/s12917-026-05750-y","DOIUrl":"10.1186/s12917-026-05750-y","url":null,"abstract":"<p><strong>Background: </strong>Atypical manifestations with a podal distribution have been occasionally reported in the USA, the UK and Israel in the early 2000s. The lesions were characterised by a tumor-like progression with a lack of remission over months and seemed to affect mostly older lambs and adult sheep, and ultimately led to euthanasia. Here, we report a case from Germany with similar findings, raising the question of whether this presentation may represent a distinct syndrome associated with orf virus infection in adult animals.</p><p><strong>Case presentation: </strong>In February 2024, an eleven-month-old lamb showed extensive non-resolving proliferative lesions on all four legs around the coronary bands. After euthanasia, pathomorphological and molecular analyses of the affected area were performed. Histologically, the haired skin showed prominent vacuolation and swelling of keratinocytes of the stratum spinosum, reticular degeneration, and extensive epidermal proliferation with eosinophilic cytoplasmic inclusion bodies indicating a poxvirus infection. Transmission electron microscopy and PCR for the B2L and RPA gene confirmed an infection with the orf virus. Phylogenetic analysis revealed that the identified German orf strain was most closely related to an Iranian strain. The strains only differed by one amino acid (A98E) in the B2L open reading frame.</p><p><strong>Conclusions: </strong>The observed podal tumor-like lesion did not spontaneously resolve and ultimately led to euthanasia of the lamb due to severe lameness. Host and viral factors may play a role in this unusual presentation. The close genetic relationship between the German strain and the Iranian IRFH9 strain is noteworthy but remains elusive. Full- genome sequencing will be required to better understand the evolutionary origins of the viral strain. This appears to be the first report of an atypical manifestation of CE in Germany.</p>","PeriodicalId":9041,"journal":{"name":"BMC Veterinary Research","volume":"22 1","pages":""},"PeriodicalIF":3.1,"publicationDate":"2026-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13401306/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148590410","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Detection of β-lactam and chloramphenicol resistance genes in Gram-negative bacteria isolated from Clarias gariepinus in selected aquaculture farms.","authors":"Yusuf Abdurrahman, Ayodele Timilehin Adesoji, Ado A","doi":"10.1186/s12917-026-05737-9","DOIUrl":"10.1186/s12917-026-05737-9","url":null,"abstract":"<p><strong>Background: </strong>Antibiotic-resistant bacteria (ARB) and their corresponding antibiotic resistance genes (ARGs) are of global public health concern. Documented information on the epidemiology and spread of ARB and ARGs among fish farms in Katsina State, Nigeria is scarce. This study isolated and characterized selected ARB and ARGs from Gram-negative bacteria in fish from aquaculture farms within Katsina State, Nigeria.</p><p><strong>Methodology: </strong>Hundred apparently healthy C. gariepinus fish samples were randomly collected from five (A, B, C, D, E) aquaculture farms. Gram-negative bacteria were isolated from samples' intestines using selective culture media. Isolates were putatively identified using biochemical characterization. Susceptibility of isolates to antimicrobial agents were determined by disk diffusion method while resistance to at least one antibiotic agent in three classes of antibiotics were categorized as Multi-Drug Resistant (MDR). MDR isolates' identities were further confirmed by Vitek-2 compact system. Beta-lactam and chloramphenicol resistance genes were amplified among all MDR isolates using PCR amplification.</p><p><strong>Results: </strong>Forty-Nine Gram-negative bacteria were isolated. Proteus spp was the most prevalent among samples from farms A (12.24%), D (8.16%) and E (12.24%) while E. coli was highest (8.16%) on farm C. Among the isolates, higher resistance to each of chloramphenicol (48.9%) and cefoxitin (36.7%) compared to gentamicin (2%) and ciprofloxacin (8.2%) was observed. Five (10.20%) MDR isolates were identified while vitek-2 identified Proteus mirabilis (2/5, 40%) and Sphingomonas paucimobilis (2/5, 40%) as the most prevalent MDR bacteria. ARGs detected include: beta-lactam (bla_TEM (4/5), bla_SHV (3/5), bla_CTX-M (1/5)) and chloramphenicol (Cat1 (1/5)) resistance genes.</p><p><strong>Conclusion: </strong>Presence of ARGs among Gram-negative bacteria from this study highlights a potential public health concern. Hence, urgent need to monitor and control antibiotics use within aquaculture to mitigate the spread of ARB and ARG to consumers through the food chain is urgently important within the study localities.</p>","PeriodicalId":9041,"journal":{"name":"BMC Veterinary Research","volume":"22 1","pages":""},"PeriodicalIF":3.1,"publicationDate":"2026-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13540951/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148879044","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Subcutaneous platelet-rich plasma and topical graphitic carbon nitride in a lanolin carrier enhance healing of surgically induced cutaneous wounds in dogs.","authors":"Mona N Wafy, Elham A Hassan, Samar Saeed, Marwa S Khattab, Huda O AbuBakr, Ashraf M Abu-Seida","doi":"10.1186/s12917-026-05739-7","DOIUrl":"10.1186/s12917-026-05739-7","url":null,"abstract":"<p><strong>Background: </strong>Graphitic carbon nitride nanoparticles (g-C3N4) are a promising material in wound healing; however, their effects and potential synergy with PRP (platelet-rich plasma) remain unexplored. This study evaluates the single and synergistic effects of PRP injection and g-C3N4 nanoparticle ointment on wound healing in dogs. Six full-thick skin wounds per group (a total of 6 groups) were surgically created on the chest region of six adult mongrel dogs, with each dog having three bilateral chest wounds. Wounds were treated with either normal saline, lanolin dressings, PRP infiltration, PRP infiltration combined with lanolin dressings, g-C3N4 NPs ointment, or PRP infiltration with g-C3N4 NPs application. Healing progression was monitored, with the evaluation of epithelialization and wound contraction. Total antioxidant capacity (TAC), malondialdehyde (MDA), and PDGFβ concentration were measured in wound fluid. Matrix extracellular phosphoglycoprotien (MEPE) and transforming growth factor beta (TGF-β) gene expressions were assessed in skin biopsies at 0, 5, 10 and 20 days. Wound histopathology and tumor necrosis factor alpha (TNF-α) immunohistochemistry (IHC) were performed.</p><p><strong>Results: </strong>Both PRP and PRP-g-C3N4 nanoparticles significantly enhanced healing. PRP alone greatly improved regeneration, yet with increased oxidative stress. These included maximal healing, epithelialization, high levels of gene expression, efficient inflammation control and well-organised granulation tissue and collagen bundles. PRP-g-C3N4 NPs offered a more balanced profile, exhibiting minimal lipid peroxidation, superior antioxidant preservation, and moderate but sustained regeneration.</p><p><strong>Conclusion: </strong>Although PRP is excellent for quick tissue repair, PRP-g-C3N4 NPs might be better suited for situations requiring oxidative stability and controlled inflammation.</p>","PeriodicalId":9041,"journal":{"name":"BMC Veterinary Research","volume":"22 1","pages":""},"PeriodicalIF":3.1,"publicationDate":"2026-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13401319/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148590340","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}