BMC Veterinary Research最新文献

{"title":"Presence and shedding dynamics of Atypical Porcine Pestivirus (APPV) in semen of breeding boars: implications for virus transmission in pig production.","authors":"Anna Bergfeldt, Frida H Aae, Mette Myrmel, Birgit Ranheim, Maria Stokstad, Randi Sørby","doi":"10.1186/s12917-026-05514-8","DOIUrl":"https://doi.org/10.1186/s12917-026-05514-8","url":null,"abstract":"<p><strong>Background: </strong>Atypical porcine pestivirus (APPV), genus Pestivirus, is the causative agent of congenital tremor type A-II, a neurological disease in newborn piglets that is characterized by tremors. Although the virus can spread horizontally, clinical disease is observed only after in utero infection. While venereal transmission has been proposed as a potential route, the pathogenesis and transmission dynamics of APPV remain incompletely understood. In this study, we aimed to investigate the presence of APPV in semen from Norwegian breeding boars, its temporal shedding patterns, and the potential impact of APPV on semen producing organs.</p><p><strong>Results: </strong>An initial screening of 110 breeding boars revealed that 50.9% (56/110) were APPV-positive in semen, as determined by reverse transcription quantitative PCR (RT-qPCR). The virus load in semen was highly variable, with concentrations up to 4.0 × 10^6 genomic copies (GC)/mL. Extended sampling of a smaller subset of APPV-positive boars (n = 19) revealed that virus shedding in semen typically persisted for less than 3 months, although intermittent shedding for up to 7 months was observed in one boar. At slaughter, the virus was detected in reproductive organs and accessory glands - even in boars with negative semen and serum samples - but it was not associated with histopathological lesions.</p><p><strong>Conclusions: </strong>As artificial insemination (AI) is widely used in all tiers of pig production, APPV-positive semen from breeding boars may represent an important source of virus transmission. To assess the viability of APPV in AI semen and clarify its potential role in transmission, further research, such as virus isolation for infectivity studies and experimental infections in naïve sows, are required.</p>","PeriodicalId":9041,"journal":{"name":"BMC Veterinary Research","volume":" ","pages":""},"PeriodicalIF":2.6,"publicationDate":"2026-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147761466","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A comparative study of fluoroscopy-guided closed reduction with 3D-printed guide-assisted cannulated screw fixation versus open surgery with a lag screw fixation in 32 cats with sacroiliac luxation-fracture.","authors":"Kamil Serdar İnal, Can Nacar, Elif Bağatır Kurban, Taylan Önyay","doi":"10.1186/s12917-026-05497-6","DOIUrl":"https://doi.org/10.1186/s12917-026-05497-6","url":null,"abstract":"","PeriodicalId":9041,"journal":{"name":"BMC Veterinary Research","volume":" ","pages":""},"PeriodicalIF":2.6,"publicationDate":"2026-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147761283","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Changing the route of GnRH administration according to the initial cyclic phase of the Ovsynch protocol may optimize reproductive outcomes in dairy cows.","authors":"Murat Abay, Gökhan Uyanık, Aytaç Akçay, Esra Canooğlu, Kutlay Gürbulak, Tayfur Bekyürek, Ömer Orkun Demiral","doi":"10.1186/s12917-026-05525-5","DOIUrl":"https://doi.org/10.1186/s12917-026-05525-5","url":null,"abstract":"<p><strong>Background: </strong>This study aimed to investigate the effects and interactions of gonadotropin-releasing hormone (GnRH) analogues (lecirelin and buserelin), administration routes (intramuscular and epidural), and initial estrus cycle phases (follicular and luteal) on fertility outcomes in cyclic dairy cows subjected to the Ovsynch protocol.</p><p><strong>Results: </strong>A total of 406 multiparous Holstein cows were randomly assigned to four groups: intramuscular lecirelin (L_im, n = 102), intramuscular buserelin (B_im, n = 102), epidural lecirelin (L_epi, n = 101), and epidural buserelin (B_epi, n = 101). During the Ovsynch protocol, the first GnRH treatment was administered on D0, followed by PGF₂α on D7, the second GnRH treatment on D9, and fixed-time artificial insemination (FTAI) 20 h later on D10. At each GnRH administration, cows received either 62.5 µg lecirelin acetate or 10 µg buserelin acetate by the intramuscular or epidural route. Blood samples for serum progesterone (P₄) determination were collected on D0, D7, D9, D10, and D13. The initial cyclic phase was determined on D0 by ovarian ultrasonography and serum P₄ concentration. Pregnancy status was assessed on D30 after FTAI, and pregnancy losses on D60. No significant differences in pregnancy or pregnancy-loss rates were detected between GnRH analogues or routes of administration, irrespective of the initial cyclic phase (P > 0.05). Likewise, within each initial cyclic phase, the route of administration did not significantly affect pregnancy rate (P > 0.05), although opposite numerical trends were observed. Pregnancy rates were numerically higher after intramuscular administration in cows starting the protocol during the luteal phase (44.31% vs. 31.95%, P = 0.083), but numerically higher after epidural administration in those starting during the follicular phase (46.66% vs. 34.48%, P = 0.083). Epidural GnRH administration was also associated with higher P₄ concentrations on D13 (P < 0.001).</p><p><strong>Conclusion: </strong>Consequently, although changing the GnRH analogue and administration route did not significantly affect overall fertility outcomes, the findings suggest a possible phase-dependent relationship between administration route and pregnancy outcome. However, this trend requires confirmation in further studies.</p>","PeriodicalId":9041,"journal":{"name":"BMC Veterinary Research","volume":" ","pages":""},"PeriodicalIF":2.6,"publicationDate":"2026-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147761440","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Detection and differentiation of pestivirus antibodies in sheep and suckler cow herds in Southern Germany.","authors":"Judith Köster, Karla Schneider, Kerstin Wernike","doi":"10.1186/s12917-026-05522-8","DOIUrl":"10.1186/s12917-026-05522-8","url":null,"abstract":"<p><strong>Background: </strong>Pestiviruses such as bovine viral diarrhea virus (BVDV) or the ovine border disease virus (BDV) are not strictly species-specific and can be transmitted between related animal species. Due to serological cross-reactivity, antibodies against e.g. BDV may interfere with BVDV diagnostics. To assess the impact of non-BVDV pestiviruses on BVDV surveillance during the transition period from virological to serological testing in a region free from BVDV for six years, the presence and type of pestivirus antibodies in sheep and cattle in Southern Germany were investigated.</p><p><strong>Results: </strong>Pestivirus antibodies were detected in 15.1% of the examined sheep farms by ELISA and identified as BDV-specific by virus neutralization test. No BVDV-specific antibodies were detected in sheep, consistent with the low prevalence of BVDV in cattle in the investigated region. In cattle, 8.1% of farms and 1.7% of animals were tested positive for pestivirus antibodies by ELISA. Most antibodies were differentiated as BVDV-specific and in those cases, all samples originated from animals that were at least 75 months old, with an average age of 183 months. However, BDV-specific antibodies were detected in six cattle farms.</p><p><strong>Conclusions: </strong>BDV seems to be currently the predominant pestivirus in sheep in Southern Germany, while interspecies transmission to cattle appears to be rare. Nevertheless, the presence of BDV-specific antibodies in cattle is relevant for serological BVDV surveillance due to serological cross-reactivity, particularly in the context of declining BVDV seroprevalence.</p>","PeriodicalId":9041,"journal":{"name":"BMC Veterinary Research","volume":" ","pages":""},"PeriodicalIF":2.6,"publicationDate":"2026-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13134168/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147761375","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prevalence and mobile genetic element-mediated dissemination of antimicrobial resistance along the swine industry chain of Guizhou, China.","authors":"Yanan Zhang, Lihong Luo, Ting Li, Yong Pan, Lexin Xie, Danping Dai, Jinge Xu, Chuanzhen Zhang","doi":"10.1186/s12917-026-05490-z","DOIUrl":"https://doi.org/10.1186/s12917-026-05490-z","url":null,"abstract":"","PeriodicalId":9041,"journal":{"name":"BMC Veterinary Research","volume":" ","pages":""},"PeriodicalIF":2.6,"publicationDate":"2026-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147761461","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular and pathological characteristics of co-infection with PRRSV-1 and PRRSV-2 recombinant strains in a pig farm in Xinjiang, China.","authors":"Shuhua Liu, Zhen Zhang, Xin Chen, Baihe Ma, Meiliang Guo, Fanxin Liang, Lianrui Li","doi":"10.1186/s12917-026-05521-9","DOIUrl":"https://doi.org/10.1186/s12917-026-05521-9","url":null,"abstract":"","PeriodicalId":9041,"journal":{"name":"BMC Veterinary Research","volume":" ","pages":""},"PeriodicalIF":2.6,"publicationDate":"2026-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147761542","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multiple congenital malformations in a litter of feline fetuses associated with gestational exposure to a synthetic estrus-suppressing progestin.","authors":"Wellida Karinne Lacerda, Ana Letícia Pereira Fernandes, Mônica Shinneider de Sousa, Pedro Lucas Chaves de Gusmão, Lucas Rannier Ribeiro Antonino Carvalho, Ricardo Barbosa Lucena","doi":"10.1186/s12917-026-05506-8","DOIUrl":"10.1186/s12917-026-05506-8","url":null,"abstract":"","PeriodicalId":9041,"journal":{"name":"BMC Veterinary Research","volume":"22 1","pages":""},"PeriodicalIF":2.6,"publicationDate":"2026-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13122987/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147761604","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ultrasonographic features of a pulmonary artery pseudoaneurysm secondary to a migrating intrathoracic grass awn in a dog: a case report.","authors":"Michał Gruss, Anna Łukomska, Maciej Głębicki, Maciej Gogulski, Roland Kozdrowski","doi":"10.1186/s12917-026-05517-5","DOIUrl":"https://doi.org/10.1186/s12917-026-05517-5","url":null,"abstract":"","PeriodicalId":9041,"journal":{"name":"BMC Veterinary Research","volume":" ","pages":""},"PeriodicalIF":2.6,"publicationDate":"2026-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147761470","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The effect of in ovo stimulation with oligosaccharides on oxidative stress markers, enzymatic activity, and mucin concentration in the intestinal mucosa of broiler chickens.","authors":"Adrianna Konopka, Marcin Barszcz, Anna Tuśnio, Ewa Święch, Kamil Gawin, Natalia Frączek, Aleksandra Bełdowska, Jakub Biesek, Mirosław Banaszak, Aleksandra Dunisławska, Maria Siwek","doi":"10.1186/s12917-026-05452-5","DOIUrl":"https://doi.org/10.1186/s12917-026-05452-5","url":null,"abstract":"","PeriodicalId":9041,"journal":{"name":"BMC Veterinary Research","volume":" ","pages":""},"PeriodicalIF":2.6,"publicationDate":"2026-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147761491","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"CRISPR/Cas12a-assisted visual and on-site detection of porcine circovirus type 2.","authors":"Xun Liao, Junxiu Zhou, Ying Shan, Xiaoliang Li, Yong Peng","doi":"10.1186/s12917-026-05513-9","DOIUrl":"https://doi.org/10.1186/s12917-026-05513-9","url":null,"abstract":"","PeriodicalId":9041,"journal":{"name":"BMC Veterinary Research","volume":" ","pages":""},"PeriodicalIF":2.6,"publicationDate":"2026-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147761383","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}