BMC Veterinary Research最新文献

{"title":"Pasteurella multocida filamentous hemagglutinin B1 (fhaB1) gene is not involved with avian fowl cholera pathogenesis in turkey poults.","authors":"Rohana P Dassanayake, Robert E Briggs, Bryan S Kaplan, Harish Menghwar, Carly Kanipe, Eduardo Casas, Fred M Tatum","doi":"10.1186/s12917-025-04668-1","DOIUrl":"10.1186/s12917-025-04668-1","url":null,"abstract":"<p><strong>Background: </strong>Pasteurella multocida is a Gram-negative coccobacillus and is the causative agent of fowl cholera in avian species. P. multocida expresses two large filamentous hemagglutinin (FhaB) proteins encoded by fhaB1 and fhaB2 genes. Previously, it was demonstrated that P. multocida FhaB2 is an important virulence factor in the development of fowl cholera disease. In the current study, we examined the potential role of FhaB1 in fowl cholera disease development. An fhaB1 deletion mutant, devoid of foreign DNA, was constructed using a temperature sensitive plasmid in a well-characterized P. multocida avian strain P-1059 (A:3).</p><p><strong>Results: </strong>Real-time PCR assay confirmed the expression of full-length fhaB1 mRNA in the wild-type parent strain and truncated fhaB1 mRNA in the ΔfhaB1 mutant strain. Both parent and the mutant strain produced biofilm; however, the ΔfhaB1 mutant produced significantly lower amounts of biofilm. Turkey poults were challenged intranasally and intramuscularly to assess the virulence of the fhaB1 mutant and the wild-type parent strains. Contrary to our expectation, inactivation of fhaB1 did not reduce virulence by either challenge route.</p><p><strong>Conclusions: </strong>These findings indicate that this large and highly conserved FhaB1 protein is not necessary for the development of acute fowl cholera disease in turkeys.</p>","PeriodicalId":9041,"journal":{"name":"BMC Veterinary Research","volume":"21 1","pages":"207"},"PeriodicalIF":2.3,"publicationDate":"2025-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11938644/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143717940","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Long term use of lokivetmab (Cytopoint®) in atopic dogs.","authors":"Margaret Gober, Deb Amodie, Marnie Mellencamp, Andrew Hillier","doi":"10.1186/s12917-025-04645-8","DOIUrl":"10.1186/s12917-025-04645-8","url":null,"abstract":"<p><p>Lokivetmab (Cytopoint®, Zoetis) has been shown to be effective for the short-term treatment of dogs with allergic and atopic dermatitis but there are no studies at US label dosing (at least 2 mg/kg every 4-8 weeks as necessary) which evaluate long-term usage. The objective of this study was to follow a cohort of dogs receiving lokivetmab to treat their canine atopic dermatitis (CAD) over 12 months. The initial phase of this interventional cohort study evaluated a dog's pruritus following monthly injections (up to 3 injections) of lokivetmab. Dogs who achieved pruritus < 36 mm using a Pet Owner Pruritus Visual Analogue Scale (PVAS) scoring system during the initial phase, were included in this study. Dogs received lokivetmab injections per the US label every 4-8 weeks and returned on days 180 and 365 (± 7 days) after their initial Day 0 for examination by investigators. Pet owners were asked to complete an electronic PVAS assessment every 2 weeks. At each visit, investigators completed a Canine Atopic Dermatitis Extent and Severity Index (CADESI-4) score and VetVAS to measure skin lesion scores. There were 87% (64/75) of dogs who maintained a PVAS below their baseline PVAS on Day 0. Over the course of the study, 88% (65/75) of dogs obtained a mean PVAS below 36 mm. Of those dogs, 31% (23/75) achieved a biweekly PVAS that was below 36 for the entirety of the study with 11% (8/75) having a biweekly PVAS score that stayed below 20 (considered normal dog level of pruritus) for the entire study. Most owners (93%; 64/49) were satisfied with lokivetmab with 88% planning to continue lokivetmab usage. The majority (80%; 55/69) of pet owners reported they were able to reduce the use of other products while their dog was using lokivetmab, and 87% (60/69) of owners found caring for their dog's atopic dermatitis was easier with lokivetmab compared to prior treatments.</p>","PeriodicalId":9041,"journal":{"name":"BMC Veterinary Research","volume":"21 1","pages":"203"},"PeriodicalIF":2.3,"publicationDate":"2025-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11938560/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143708385","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessment of physical facilities and detection of post-mortem lesions in public abattoirs in selected districts of Wolaita zone, Ethiopia.","authors":"Melese Yilma Zaba, Asefa Asmare, Joseph P Cassidy, Stephen V Gordon","doi":"10.1186/s12917-025-04697-w","DOIUrl":"10.1186/s12917-025-04697-w","url":null,"abstract":"<p><strong>Background: </strong>Participatory abattoir appraisals and observational analysis were conducted in selected local abattoirs in the Wolaita, Ethiopia with the aims of assessing (i) the physical facilities available in these abattoirs and (ii) the detection of post-mortem gross lesions upon routine meat inspection that may have been due to infectious diseases.</p><p><strong>Methods: </strong>Our study surveyed public abattoirs to evaluate meat inspection services and facility standards. Gross lesions in slaughtered animals were categorized by consistency, prioritized using a scoring matrix, and analyzed for anatomical distribution. Concordance in prioritization was assessed with Kendall's W test.</p><p><strong>Results: </strong>Our investigation revealed that almost all public abattoirs surveyed had substandard physical facilities for conducting meat inspection services. According to statistics from the studied abattoirs, 26.6% of slaughtered animals exhibited at least one grossly visible lesion, of which 65% were considered by the local veterinary inspectorate to have potential animal or public health significance. Among the identified lesions, 10% were classified as having a 'soft' consistency (e.g., cystic or caseous/abscess), while 5% were categorized as 'hard' (e.g., fibrotic, firm, fibrous, or mineralized). The remaining 85% of lesions were uncategorized. Using a prioritization matrix scoring method, the overall mean proportional priority rank data indicated that 'mineralized/calcified' hard lesions were assigned the highest priority (0.67), followed by 'cystic' lesions (0.58). The evidence for group concordance in prioritization was moderate (W = 0.275; p = 0.019). Regarding anatomical distribution, the survey groups ranked gross lesions in the lungs and associated mediastinal and bronchial lymph nodes as the top priority (0.67). Lesions in the small intestine and mesenteric lymph nodes (0.33) were ranked second, followed by lesions in the hepatic lymph nodes (0.42). There was strong concordance in the overall mean ranking of these lesion sites within the survey groups (W = 0.518; p = 0.0001). Some of the gross lesions detected in this study may pose a potential zoonotic risk (e.g. tuberculosis).</p><p><strong>Conclusions: </strong>This study highlights how infrastructural deficiencies and operational procedures in abattoirs can contribute to poor quality and potentially diseased meat entering the human food chain, features which need to be addressed locally to safeguard public health.</p>","PeriodicalId":9041,"journal":{"name":"BMC Veterinary Research","volume":"21 1","pages":"208"},"PeriodicalIF":2.3,"publicationDate":"2025-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11938597/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143717934","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Association between sperm DNA fragmentation and fertility parameters in farm animals: a systematic review and meta-analysis.","authors":"Kenneth Owoicho Abah, Zuzanna Ligocka-Kowalczyk, Joy Iyojo Itodo, Grace Ameh, Agnieszka Partyka, Wojciech Nizanski","doi":"10.1186/s12917-025-04652-9","DOIUrl":"10.1186/s12917-025-04652-9","url":null,"abstract":"<p><strong>Background: </strong>Accurately predicting male fertility is crucial for the animal breeding industry due to its significant economic implications. Existing literature suggests that mammalian fertility is partially dependent on sperm DNA integrity. However, routine semen analysis often fails to detect DNA damage and does not consistently correlate with field fertility outcomes. While assessing sperm DNA integrity provides valuable biological insights, its role in diagnosing animal infertility remains uncertain.</p><p><strong>Results: </strong>This meta-analysis evaluated the association between sperm DNA fragmentation (SDF) and fertility in farm animals. Comprehensive searches were conducted using PubMed, Google Scholar, and Springer Link Library, with results stratified by animal species and SDF detection methods. Across 30 studies, the overall correlation coefficient (COR) between SDF and male fertility was -0.46 (95% confidence interval [CI]: -0.54 to -0.37; Z = -8.97; p < 0.001). A significant association was observed in bulls (COR = -0.47; 95% CI: -0.54 to -0.40; Z = -11.13; p < 0.001) and stallions (COR = -0.54; 95% CI: -0.72 to -0.29; Z = -3.83; p < 0.001), whereas no significant relationship was found in boars (COR = -0.19; 95% CI: -0.37 to 0.01; Z = -1.84; p = 0.07). The effect of SDF on male fertility was analyzed in 15 studies, demonstrating significantly higher SDF values in low-fertility animals compared to high-fertility groups (SMD = 0.85; 95% CI: 0.68 to 1.01; Z = 10.07; p < 0.001). This pattern was observed in both bulls (SMD = 1.21; 95% CI: 0.85 to 1.57; Z = 6.59; p < 0.001) and stallions (SMD = 0.64; 95% CI: 0.44 to 0.85; Z = 6.14; p < 0.001) subgroups.</p><p><strong>Conclusions: </strong>These findings suggest that incorporating SDF assays into breeding soundness evaluations could enhance the accuracy of selecting high-quality breeding males for artificial breeding programs. However, further research with adequately powered studies, standardized methodologies, and appropriate sample sizes is necessary to fully elucidate the impact of elevated SDF on fertility in farm animals.</p>","PeriodicalId":9041,"journal":{"name":"BMC Veterinary Research","volume":"21 1","pages":"204"},"PeriodicalIF":2.3,"publicationDate":"2025-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11938742/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143708327","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The impact of in ovo injection of cluster bean peptide on hatchability, growth performance, carcass characteristics, digestive enzymes, and blood indices of broiler chickens.","authors":"Hussein H El-Fakhrany, Zenat A Ibrahim, Elwy A Ashour, Mahmoud Alagawany","doi":"10.1186/s12917-025-04636-9","DOIUrl":"10.1186/s12917-025-04636-9","url":null,"abstract":"<p><p>The administration of bioactive short peptides through in ovo feeding can improve the overall health and performance of broiler chickens for the poultry industry. Additionally, bioactive peptides possess biological features that have the potential to be beneficial in preventing many metabolic illnesses; hence, the ingestion of these peptides holds the potential to be advantageous for human health. In light of this, the current work aimed to study the impacts of in ovo feeding during the late stages of embryonic development with cluster bean peptide (CBP) on the hatchability, productive performance, lipid profile, liver and kidney functions, immunological response, and antioxidant status of broilers. Six hundred and forty-eight (648) fertilized Ross 308 broiler breeder eggs were used in this study. To remove infertile eggs and dead embryos, the eggs were manually candled on 7 and 17 day of incubation (DOI). On the 18.5th DOI, the eggs were separated into four treatment groups (156 eggs/each), and the first group did not receive any treatment and represented the negative control (NC). Meanwhile, the other treatment groups were injected into the amnion membrane. The second group was only subjected to needle penetration and represented the positive control (PC). The third group was denoted by the letter T1 and received an injection of 1 mg CBP/egg. The fourth group was denoted by the letter T2 and received an injection of 2 mg CBP/egg. In ovo feeding by CBP exhibited significant improvements in the body weight of newly hatched chicks, particularly at the 2 mg CBP level. The administration with CBP did not significantly affect the carcass characteristics of 28-day-old broilers. In ovo-administrated groups with CBP, higher plasma concentrations of total protein and its fractions were observed at hatch and on day 28 of age. In ovo treatment with CBP, blood lipid profile parameters significantly improved at hatch and 28 days of age. Liver and kidney function parameters were improved in response to the in ovo administration with CBP in newly hatched chicks and on day 28 of age. Blood levels of glutathione (GSH) and superoxide dismutase (SOD) were considerably higher in the in ovo-administered groups with CBP; while levels of malondialdehyde (MDA) were significantly reduced due to CBP administration. The activity of digestive enzymes in blood plasma was decreased in newly hatched chicks but increased in 28-day-old broilers in response to in ovo administration with CBP. There was an improvement in the immunological response of hatched chicks from groups injected with CBP, particularly the T2 group (2 mg CBP), as evidenced by increased IgM and IgG levels. The findings presented here indicate that the in ovo administration with CBP, specifically at a dosage of 2 mg, improved growth performance and immune and antioxidant functions.</p>","PeriodicalId":9041,"journal":{"name":"BMC Veterinary Research","volume":"21 1","pages":"200"},"PeriodicalIF":2.3,"publicationDate":"2025-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11934734/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143699452","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"De-novo assembled mitochondrial genome of Bhadawari buffalo (Bubalus bubalis) reveals close divergence to Egyptian buffalo.","authors":"Ameya Santhosh, Vikas Vohra, Ravi Kumar Gandham, Rani Alex, Gopal Gowane","doi":"10.1186/s12917-025-04675-2","DOIUrl":"10.1186/s12917-025-04675-2","url":null,"abstract":"<p><strong>Background: </strong>The objective of this study was to assemble the mitochondrial genome of Bhadawari buffalo and do phylogenetic analysis of it. We assembled the complete mitochondrial sequence of Bhadawari buffalo de novo from short Illumina sequences generated from the paired-end library. Phylogenetic analysis was done on 24 assembled mitochondrial genomes from the Bovidae family using the Maximum Likelihood method and General Time Reversible Substitution Model.</p><p><strong>Results: </strong>The complete circularized mitochondrial assembly of Bhadawari consists of 16,358 bp and this encodes 36 genes (13 Protein coding 22 tRNA and 2 rRNA sequences). The phylogenetic analysis with other published mitochondrial assemblies revealed a divergence time of Bubalis and Syncerus from the Bos group about 6.4 million years from today. The divergence of Syncerus-Bubalis, Mediterranean occurred at about 4.17 MYA and 1.84 MYA respectively. The Indian and Egyptian buffaloes were grouped in a subclade while Chinese and Iraqi buffaloes were in another. The Indonesian and Indian buffaloes diverged from the Chinese subclade by about 0.72 MYA.</p><p><strong>Conclusions: </strong>The study suggests an independent selection and improvement of the Asiatic buffaloes in different geographical locations 10,000-40,000 years from today which coincides with the domestication period of buffaloes. The Bhadawari and Egyptian water buffalo revealed a more recent divergence.</p>","PeriodicalId":9041,"journal":{"name":"BMC Veterinary Research","volume":"21 1","pages":"202"},"PeriodicalIF":2.3,"publicationDate":"2025-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11934611/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143708329","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Morphometric analysis of ventricular indexes and intracranial cerebrospinal fluid spaces in the brain using magnetic resonance imaging healthy in Van cats.","authors":"Veysel Delibaş, Cemil Göya","doi":"10.1186/s12917-025-04674-3","DOIUrl":"10.1186/s12917-025-04674-3","url":null,"abstract":"<p><strong>Background: </strong>Ventricular indexes are defined as a numerical marker of ventricular dimensions in domestic mammals. The anatomical size of the brain ventricles has been the subject of many studies so far and has been accepted as a potential indicator of many brain disorders in the clinical field. Currently, the number of studies describing the morphometry of the brain ventricular system in cats is limited. Furthermore, no studies have been conducted specifically on indexes characterizing the numerical compatibility of the brain and brain ventricles in cats. The aim of this study was to reveal the morphometric status of intracranial cerebrospinal fluid spaces and ventricular indexes in healthy Van cats by magnetic resonance imaging method.</p><p><strong>Results: </strong>For this retrospective study, T2-weighted magnetic resonance imaging (MRI) scans were performed at 1.5 T on 20 (10 male and 10 female) Van cats, under general anaesthesia. The animals were at the age of mean 4 (3-5 age). All Van cats were selected from individuals who had not undergone any surgical procedures, were not neutered, and had no visible anomalies. The statistical analysis of first, descriptive statistics such as mean and standard deviation were calculated. In line with the mean results obtained, the difference between sex was examined statistically. 'A Mann-Whitney U test' was applied to detect sex differences in measurement parameters in the study. The results are as follows (mean ± standard deviation): İntracranial cerebrospinal fluid spaces measurements: R-fss: 1.02 ± 0.19 mm, L-fss: 1.03 ± 0.18 mm, A-if: 1.06 ± 0.26 mm, R-sf: 1.38 ± 0.32 mm, L-sf: 1.37 ± 0.27, V₁: 4.26 ± 0.53 mm. Indexes: Fourth ventricle: 15.95 ± 1.73%, Bifrontal: 17.45 ± 1.78%, Bioccipital: 47.53 ± 9.36%, Evans: 13.76 ± 2.93%, Lateral ventricle: 35.41 ± 2.50%, Callosal angle: 85.06°±4.42°.</p><p><strong>Conclusions: </strong>The present study provides baseline values of intracranial cerebrospinal fluid spaces and linear indexes of the ventricles in the Van cats. The acquisition of these data contributes to filling the knowledge void on important anatomical and morphological features of the Van cats brain.</p>","PeriodicalId":9041,"journal":{"name":"BMC Veterinary Research","volume":"21 1","pages":"198"},"PeriodicalIF":2.3,"publicationDate":"2025-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11934450/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143699448","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Maize stover burning exposure accountable for remarkable environmental and health risk in broiler chickens.","authors":"Manal A M Mahmoud, Abd El-Aziz A Said, Hanan H Abd-Elhafeez, Soha A Soliman, Usama T Mahmoud","doi":"10.1186/s12917-025-04476-7","DOIUrl":"10.1186/s12917-025-04476-7","url":null,"abstract":"<p><strong>Background: </strong>Biomass burning presents significant environmental and health problems worldwide. Health effects on broilers (as an animal model) exposed to intensive maize stover burning (MSB) were studied. Carbon monoxide (CO) and fine particulate matter (PM2.5) were estimated during the MSB season. Sixty apparently healthy broilers from 12 farms were included for blood-gas analysis, bilirubin, and liver enzyme analysis. In addition, histopathological changes of the lung, liver, and heart were investigated.</p><p><strong>Results: </strong>Highly significant differences for CO and PM2.5 levels, hemoglobin (Hb), and hematocrit (Hct) values during MSB season were found compared to burning free events which resulted in higher incidence of blood coagulation and cardiovascular diseases risk. Highly significant elevations of liver enzymes were verified during MSB. Respiratory function was significantly decreased due to airway obstruction accompanied by severe tissue damage including pulmonary fibrosis (39%) and metaplasia. Pulmonary and hepatic blood vessel embolisms were indicative of systemic embolic phenomena.</p><p><strong>Conclusion: </strong>The study highlighted the substantial health risk and a threat to air quality from one season exposure to leftover straw burning. Agriculture waste burning should be banned by legislation to restore the environment and protect health.</p>","PeriodicalId":9041,"journal":{"name":"BMC Veterinary Research","volume":"21 1","pages":"199"},"PeriodicalIF":2.3,"publicationDate":"2025-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11934768/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143699478","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Changes in fecal microbiota of dairy cows with and without endometritis.","authors":"Zhi-Hai Shi, Ya-Li Lan, Zhi-Hui Qiao, Xiang-Zhou Yan, Ya-Zhou Wang, Bin Zhang, Xiao-Ya Ma, Faiz-Ul Hassan, Wen-Jia Wang, Ting-Xian Deng","doi":"10.1186/s12917-025-04580-8","DOIUrl":"10.1186/s12917-025-04580-8","url":null,"abstract":"<p><strong>Background: </strong>Endometritis is a uterine infection caused by bacterial pathogens and has detrimental effects on productive and reproductive performance in dairy cows. A large number of studies have demonstrated the association of gut microbiota with infectious diseases. However, the role of gut microbiota in dairy cows with endometritis is still poorly understood.</p><p><strong>Results: </strong>In the present study, we characterized the fecal microbial populations in the dairy cows suffering from metritis (n = 10) and healthy cows (n = 9) using the 16 S rRNA gene sequencing. Results revealed an increased abundance of Firmicutes and Bacteroidetes in the affected cows indicating the potential role of these two bacterial taxa in the pathogenesis of endometritis. The Ruminococcaceae_UCG-005 was the predominant genus while Olsenella and Succinivibrio were the most abundant genera in the cows affected with metritis. Further, the association of specific genera from Firmicutes and Bacteroidetes indicated three co-occurrence groups indicating the potential interaction of these genera in modulating the immune response, dysbiosis and inflammatory reaction. In addition, a significantly higher abundance of genes involved in the excretory system was observed in affected cows.</p><p><strong>Conclusions: </strong>Our findings provide evidence of changes in gut microbiota composition in cows suffering from metritis and advocate the need to explore the effect of commensal gut bacteria specifically co-occurring taxa in uterine inflammation and infection.</p>","PeriodicalId":9041,"journal":{"name":"BMC Veterinary Research","volume":"21 1","pages":"201"},"PeriodicalIF":2.3,"publicationDate":"2025-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11934761/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143699476","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Outcomes of non-limited versus cranial-limited extensive hemilaminectomy and durotomy in dogs with thoracolumbar intervertebral disc extrusion and presumptive progressive myelomalacia.","authors":"Yuya Nakamoto, Kyosuke Hidari, Mei Matsuo, Miwa Nakamoto","doi":"10.1186/s12917-025-04651-w","DOIUrl":"10.1186/s12917-025-04651-w","url":null,"abstract":"<p><strong>Background: </strong>Progressive myelomalacia (PMM) can occur secondary to thoracolumbar intervertebral disc extrusion (TL-IVDE) and is typically fatal. A recent study suggested that compared with dogs undergoing standard hemilaminectomy, the survival rate of dogs with presumptive PMM improved with non-limited extensive hemilaminectomy and durotomy (EHLD) conducted on the region with a hyperintense intramedullary signal on T2-weighted magnetic resonance imaging (MRI). Applying EHLD results in a large wound and entails prolonged surgical duration. The current study aimed to retrospectively compare the outcomes between an EHLD group and a limited EHLD group (EHLD-L) with TL-IVDE and presumptive PMM. EHLD-L was performed from one additional vertebral body cranial to the spinal cord parenchyma with the T2-weighted hyperintense region identified on MRI, extending caudally until spinal cord swelling and/or softening was visually confirmed during hemilaminectomy and durotomy. Twelve dogs diagnosed with TL-IVDE and presumptive PMM based on clinical features and MRI findings were retrospectively recruited. After diagnosis, seven and five dogs immediately underwent EHLD-L and EHLD, respectively. Medical records of all dogs were retrospectively reviewed, and the postoperative survival outcome, length of the hemilaminectomy window, and surgical operating time between the two groups were compared.</p><p><strong>Results: </strong>One month after the surgery, the survival rates of the EHLD-L and EHLD groups were 6/7 and 5/5, respectively. The median length of the hemilaminectomy window was 4 (range, 4-6) for the EHLD-L group and 10 (range, 6-14) for the EHLD group (p = 0.076). The mean surgical operating times were 106 (range, 80-168) minutes in the EHLD-L group and 192 (range, 128-207) minutes in the EHLD group (p = 0.030). There was no improvement in any surviving dogs' urinary continence or pelvic limb function.</p><p><strong>Conclusions: </strong>Compared with EHLD, EHLD-L can be associated with a smaller surgical wound and a shorter anesthesia time. EHLD-L did not affect the recovery of pelvic limb function or urinary continence. However, it can be an alternative to EHLD for preserving the life of dogs with presumed PMM.</p>","PeriodicalId":9041,"journal":{"name":"BMC Veterinary Research","volume":"21 1","pages":"197"},"PeriodicalIF":2.3,"publicationDate":"2025-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11929197/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143690912","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}