BMC Pregnancy and Childbirth最新文献

{"title":"Effect of a multifaceted intervention with audit and feedback on low-risk childbirth practice: a multicentre prospective study.","authors":"Kayo Ueda, Mai Takeshita, Yoshimitsu Takahashi, Hatoko Sasaki, Naoki Ozu, Takeo Nakayama","doi":"10.1186/s12884-025-07681-2","DOIUrl":"https://doi.org/10.1186/s12884-025-07681-2","url":null,"abstract":"<p><strong>Background: </strong>Care for low-risk childbirths constitutes a large proportion of deliveries and is highly influenced by factors such as region, birthing facilities, and health care providers. Audit and feedback as a quality indicator (QI) intervention alone have limited effectiveness. Multidisciplinary approaches, including QI and organizational development, are reportedly effective; however, the impact on low-risk childbirth care remains unclear. We aimed to assess the impact of multifaceted intervention, including audit and feedback, on improving care for low-risk childbirths using QIs.</p><p><strong>Methods: </strong>We conducted a 1-year pre-post comparison targeting healthy pregnant women in four obstetric wards in Japan. The intervention included audit and feedback combined with multifaceted approaches, improvement efforts by a multidisciplinary team, and educational training on health care quality and organizational culture. The outcomes were 12 QIs. The main analysis used interrupted time-series analysis over 6 months pre- and post-intervention. We compared the 9 months pre-intervention with 3 months post-intervention in secondary analysis to assess delayed effects.</p><p><strong>Results: </strong>We included 288 women pre-intervention and 167 women post-intervention. \"The spontaneous vaginal delivery indicator showed a significant increase in slope (risk ratio [RR] 1∙08, 95% confidence interval [CI]: 1∙00-1∙16, p < 0∙05), indicating a trend-based improvement rather than an immediate change per month in the main analysis. Secondary analysis showed a significant increase in the administration of uterotonic agents during the third stage of labour (RR 1∙19, 95% CI: 1∙01-1∙41, p < 0∙05).</p><p><strong>Conclusion: </strong>The improvement effects of multifaceted interventions, including audit and feedback, using QIs for low-risk childbirths were limited. However, some indicators may improve over time, suggesting a potential delayed effect.</p><p><strong>Trial registration: </strong>Not applicable.</p>","PeriodicalId":9033,"journal":{"name":"BMC Pregnancy and Childbirth","volume":"25 1","pages":"571"},"PeriodicalIF":2.8,"publicationDate":"2025-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12076964/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144075803","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring healthcare providers' perspectives of childbirth education classes for quality of care and positive childbirth experience: an interpretative phenomenological analysis study.","authors":"Anwar Nader AlKhunaizi, Sami Abdulrahman Alhamidi, Areej Ghalib Al-Otaibi, Amany Anwar AlAbdullah, Kawther Saleh Alosaif, Meral Jehad Al Zayer","doi":"10.1186/s12884-025-07698-7","DOIUrl":"https://doi.org/10.1186/s12884-025-07698-7","url":null,"abstract":"<p><strong>Background: </strong>Pregnancy is a significant transitional life experience that can be one of the most stressful experiences in life. Childbirth education is designed to improve health behaviors and offers information on psychological and physical changes that occur in pregnancy, signs that labor has begun, hospital routines and what to expect, how to manage pain through non-pharmacological strategies, the first hours of a newborn's life, and the benefits of breastfeeding. Healthcare providers play an essential role in this education. To discover how healthcare providers view childbirth education classes, we explored their perceptions in relation to the quality of care and positive childbirth experiences.</p><p><strong>Methods: </strong>An interpretative phenomenological qualitative approach was conducted in three government hospitals in the Eastern Province of Saudi Arabia. Data collection involved conducting semi-structured interviews with 15 participants. The sample consisted of physicians, nurses, and educators, ensuring a diverse range of perspectives.</p><p><strong>Results: </strong>An interpretative phenomenological analysis was conducted for data analysis. One core category (Road to a Pleasant and Safe Journey) with three themes (Mother's Experience, Obstacles, and Struggles of Healthcare Providers, and Solutions & Suggestions) emerged from the data analysis. The study findings indicate that childbirth education programs boost maternal health and facilitate a more positive delivery experience.</p><p><strong>Conclusions: </strong>Receiving childbirth education about natural and instinctive childbirth was necessary for low-risk mothers to experience a positive childbirth experience. The education also enabled mothers to feel in control during pregnancy, birth, and postpartum. From the results of this research the following recommendations can be made: childbirth education should be mandatory for all hospitals and primary healthcare institutions in the Kingdom and its curriculum should be standardized by the ministry of health and all healthcare providers employed in maternity care should be required to attend the standardized childbirth education programs.</p>","PeriodicalId":9033,"journal":{"name":"BMC Pregnancy and Childbirth","volume":"25 1","pages":"570"},"PeriodicalIF":2.8,"publicationDate":"2025-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12076960/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144075807","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Overreliance on cultural doulas: the paradox of entrusting the communication and care of high-risk migrant women to cultural doulas.","authors":"Birgitta Essén, Ayanthi Wickramasinghe, Lise Eriksson","doi":"10.1186/s12884-025-07700-2","DOIUrl":"https://doi.org/10.1186/s12884-025-07700-2","url":null,"abstract":"<p><strong>Background: </strong>It is widely recognized that migrant women from low-income countries are considered to be a group with increased obstetric challenges. To address these challenges, cultural doulas were introduced to provide continuous emotional and practical support during childbirth in Sweden. Leveraging their shared cultural background, language skills, and understanding, the idea behind these doulas was supposed to facilitate effective communication between the woman, her partner, and healthcare staff, with the assumption that this would lead to better maternity care for migrants. The aim of this study was to explore healthcare providers reflections on the role of cultural doulas and to explore their perceptions of cultural doulas' impact on childbirth.</p><p><strong>Methods: </strong>A qualitative study was conducted in 2022, involving semi-structured interviews with 18 healthcare providers; obstetricians and midwives from two Swedish counties. The data was analyzed using reflexive thematic analysis and discourse analysis, guided by Bacchi's 'What Is the Problem Represented to Be?'</p><p><strong>Approach: </strong></p><p><strong>Results: </strong>Using Bacchi's 'What Is the Problem Represented to Be?' approach, the analysis highlights how healthcare providers interpreted cultural doulas as an asset in relation to problems in migrants' maternity care. Three key discourses that emerged were: underlying social and cultural factors, assumptions of improved outcomes and integration, and cultural doulas as informal interpreters. Instead of emphasizing medical risks, healthcare providers focused on social risks and overlooked the importance of professional training.</p><p><strong>Conclusions: </strong>Cultural doulas are recognized as valuable in addressing gaps in migrant maternity care, yet their role presents a paradox. Entrusting the care of high-risk migrant women to minimally trained non-medical professionals paradoxically risks miscommunication and compromised care quality. Insufficient training, unclear roles, and the overextension of cultural doulas further exacerbate this issue, underscoring the need for systemic reforms. To resolve this paradox and improve maternal outcomes, the maternity care system must redefine the role of cultural doulas, prioritize professional interpretation services, and implement integrated care models tailored to the evidence based medical needs of migrant women.</p>","PeriodicalId":9033,"journal":{"name":"BMC Pregnancy and Childbirth","volume":"25 1","pages":"574"},"PeriodicalIF":2.8,"publicationDate":"2025-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12076999/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144075812","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Association between late pregnancy A/G ratio and the risk of neonatal admission for neonatal hyperbilirubinemia.","authors":"Hongjuan Wei, Xin Chang, Rufeng Ji, Yinyan Tang","doi":"10.1186/s12884-025-07706-w","DOIUrl":"10.1186/s12884-025-07706-w","url":null,"abstract":"<p><strong>Objective: </strong>To investigate the association between late pregnancy A/G (Albumin to globulin) ratio and the risk of admission for neonatal hyperbilirubinemia (NHB).</p><p><strong>Methods: </strong>This cross-sectional study selected mothers in labor and their newborns delivered at Nanjing Lishui People's Hospital, from January to December 2022. Multivariate logistic regression was utilized to analyze the relationship between late pregnancy A/G ratio and the risk of admission for NHB.</p><p><strong>Results: </strong>Out of 1432 pregnant women, 15.7% of newborns were admitted for NHB. Outcome 1: Dichotomizing the A/G ratio at 1.29, the risk of NHB admission decreased by 33% (95% CI: 0.46-0.97) for every 0.1 increase in A/G ratio < 1.29. Conversely, when the A/G ratio ≥ 1.29, the risk of NHB admission increased by 16% (95% CI: 1.01-1.32) for each 0.1 increase in A/G ratio. Outcome 2: When A/G ratio was categorized into three groups using thresholds of 1.15 and 1.40, the risk of NHB admission increased by 107% (95% CI: 1.17-3.66) for G1 and 60% (95% CI: 1.16-2.19) for G3, compared to G2.</p><p><strong>Conclusion: </strong>Late pregnancy A/G ratio is closely associated with the risk of admission for NHB. A/G ratio within different ranges affects the risk of NHB in varying directions and to different extents. Monitoring the A/G ratio may help identify pregnancies at higher risk of NHB.</p>","PeriodicalId":9033,"journal":{"name":"BMC Pregnancy and Childbirth","volume":"25 1","pages":"563"},"PeriodicalIF":2.8,"publicationDate":"2025-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12070661/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143961911","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring uterine niche: a systemic review on secondary infertility rates, pathophysiological correlations, impact on assisted reproduction technology (ART), and the efficacy of surgical interventions.","authors":"D M C S Jayasundara, I A Jayawardane, T D K M Jayasingha, S D S Weliange","doi":"10.1186/s12884-025-07638-5","DOIUrl":"10.1186/s12884-025-07638-5","url":null,"abstract":"<p><strong>Background: </strong>The rising trend of cesarean sections worldwide has resulted in an increased incidence of uterine niches, a cavity formed at the cesarean scar site due to impaired tissue healing. Secondary infertility in women with uterine niches is a hot topic in obstetrics and gynecology. Therefore, the current study aims to untwist the link between secondary infertility and uterine niche, exploring the pathophysiological correlations, effects on assisted reproduction technology, and role of surgical interventions in resuming fertility.</p><p><strong>Methodology: </strong>PubMed, Cochrane Library, Embase, and Science Direct were searched systematically. Rayyan was employed as a semi-automated tool for study selection. Full-text articles in the English language were included. Systematic reviews, meta-analyses, or book chapters were excluded. Newcastle-Ottawa Scale assessed the quality of cohort and case-control studies, while the Cochrane Risk-of-bias tool evaluated randomized controlled trials. Data synthesis followed a thematic analysis.</p><p><strong>Results: </strong>35 articles from 3301 studies met the inclusion criteria. Among those, 25 were cohort studies, only one was a randomized controlled trial, and the rest had different study designs. The study quality assessment revealed average to good quality. The incidence of secondary infertility in women with uterine niches ranged from 27.37% (n = 95) to 75% (n = 16). Decreased residual myometrial thickness, chronic inflammatory changes at the niche site, and fluid accumulation within the niche cavity were identified as leading causes of secondary infertility. The uterine niche adversely affected assisted reproductive outcomes through multiple mechanisms. Various surgical interventions, including hysteroscopy, laparoscopy, or combined surgery, showed differing efficacies in restoring fertility.</p><p><strong>Conclusion: </strong>The study provides valuable insights regarding the association between secondary infertility and uterine niche. However, smaller sample sizes, retrospective nature of study designs, reliance on observational data, and heterogeneity of study reporting have limited the ability to arrive at solid conclusions. Therefore, we encourage well-designed prospective studies, including randomized controlled trials, to further explore this trending area.</p><p><strong>Register: </strong>The study protocol was prospectively registered in the International Prospective Register of Systematic Reviews (PROSPERO CRD4204526319).</p>","PeriodicalId":9033,"journal":{"name":"BMC Pregnancy and Childbirth","volume":"25 1","pages":"566"},"PeriodicalIF":2.8,"publicationDate":"2025-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12076856/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143969915","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A mitochondrial regulator protein, mitofusin 2, is elevated in the maternal blood of women with preeclampsia.","authors":"Dandan Sun, Haijin Zhang, Yanting Wu, Jiawei Zhou, Ling Ai","doi":"10.1186/s12884-025-07663-4","DOIUrl":"10.1186/s12884-025-07663-4","url":null,"abstract":"<p><strong>Objective: </strong>To investigate the expression of mitofusin 2 (Mfn2) in the placenta and peripheral blood of patients with early-onset preeclampsia (eoPE) and late-onset preeclampsia (loPE), and to evaluate its possibility as a diagnostic and therapeutic target for preeclampsia.</p><p><strong>Methods: </strong>A total of 68 pregnant women with preeclampsia in Jiaxing Maternal and Child Health Hospital from June 2022 to June 2024 were selected, including 32 patients with eoPE and 36 patients with loPE, and 68 term pregnant women as negative controls. Real-time fluorescence reverse transcription (RT-qPCR) was used to determine the expression level of Mfn2 mRNA in placenta and peripheral blood, and the expression of Mfn2 was analyzed; enzyme-linked immunosorbent assay (ELISA) was used to determine the level of Mfn2 in peripheral blood of patients, and the outcomes of pregnant women (placental weight, neonatal birth weight, 1 min Apgar) were recorded. The correlation between the level of Mfn2 in peripheral blood and the severity of preeclampsia and pregnancy outcomes was analyzed.</p><p><strong>Result: </strong>The expression of Mfn2 mRNA in the placenta tissue of the eoPE group was significantly lower than that of the term pregnancy group and the loPE group (P < 0.001), while the expression of Mfn2 in the placenta tissue of the loPE group was only lower than that of the term pregnancy group, with no significant difference(P > 0.05). The expression of Mfn2 mRNA in the peripheral blood of eoPE and loPE was significantly higher than that of term pregnancy group (all P < 0.001). In the peripheral blood, the levels of Mfn2 in eoPE group and loPE group were significantly higher than that of the term pregnancy group (all P < 0.001). The Pearson correlation analysis showed that the expression level of Mfn2 protein in peripheral blood of patients are positively correlated with blood pressure and urinary protein, and negatively correlated with neonatal birth weight and 1 min Apgar score.</p><p><strong>Conclusion: </strong>In early-onset placenta, the expression of Mfn2 is significantly lower than that in full-term pregnancy, while in late-onset eclampsia, it is not, indicating that the abnormal expression of Mnf2 in placenta is related to the occurrence period of preeclampsia and the progression of the disease; Mfn2 in peripheral blood can be used as a biological marker to evaluate the occurrence and progression of preeclampsia.</p>","PeriodicalId":9033,"journal":{"name":"BMC Pregnancy and Childbirth","volume":"25 1","pages":"567"},"PeriodicalIF":2.8,"publicationDate":"2025-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12076813/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143957243","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Differences in women's experiences of labour according to type of fetal monitoring: a quantitative analysis of an Australian national survey.","authors":"Kate M Levett, Deborah Fox, Panashe Bamhare, Rebecca Coddington, Kerry L Sutcliffe, Elizabeth Newnham, Vanessa Scarf","doi":"10.1186/s12884-025-07509-z","DOIUrl":"10.1186/s12884-025-07509-z","url":null,"abstract":"<p><strong>Intro: </strong>While monitoring of the fetal heart rate in labour is recommended, few studies have compared women's experiences of different forms of monitoring technologies, their impact on labour and perceived risks and benefits.</p><p><strong>Methods: </strong>The Women's experiences of Monitoring Baby (WOMB) study, an Australian national survey, examined experiences of intrapartum fetal monitoring in labour. This study is one of two quantitative analyses of survey responses received.</p><p><strong>Results: </strong>We received 861 valid responses. The most common form of monitoring across all hospital settings was wired CTG (53% of total). Women who used wired CTG were more likely to be primiparous (OR = 3.220, [95%CI:2.080-4.987], p < 0.001), and give birth at a private hospital (OR = 3.017 [95%CI:1.632-5.576], p < 0.001). Women who were monitored via wired CTG were more likely to use pharmacological pain management, and have an emergency caesarean section (p < 0.001), which remained significant when adjusting for epidural. Women who gave birth vaginally were more likely to have been monitored via intermittent auscultation (OR = 3.582, [95%CI:2.007-6.390], p < 0.001), and to use non-pharmacological techniques such as mobility (p < 0.001) and supportive care (p < 0.01). Of the women monitored via wired CTG 58% felt that monitoring had a negative impact on their labour.</p><p><strong>Conclusion: </strong>This study has substantial implications for research, policy and practice, including the implementation of less invasive and more humanised forms of fetal monitoring. The promotion of freedom of movement and bodily autonomy in labour is essential. This includes implementation of evidence-based practices and information about methods of fetal monitoring that support woman-centred care and optimise physiological processes.</p>","PeriodicalId":9033,"journal":{"name":"BMC Pregnancy and Childbirth","volume":"25 1","pages":"565"},"PeriodicalIF":2.8,"publicationDate":"2025-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12076947/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143958264","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"'This is like tradition, lie on your back, hold your leg, and push': understanding midwives' perspectives on their choice of labour positions in a Ugandan hospital.","authors":"Zuhaira Husna Fatma, Helle Mölsted Alvesson, Gertrude Namazzi, Josephine Babirye Kyobe, Elizabeth Ayebare","doi":"10.1186/s12884-025-07657-2","DOIUrl":"10.1186/s12884-025-07657-2","url":null,"abstract":"<p><strong>Introduction: </strong>Despite the well-documented benefits of upright positions and mobility during labour and childbirth, the adoption of these remains limited. Offering women choices in labour positions is essential for respectful maternity care. Since midwives play a pivotal role in guiding women through labour, their perspectives are crucial to the effective integration of these practices.</p><p><strong>Aim: </strong>The study aimed to understand midwives' perspectives and reasoning behind their choice of labour positions.</p><p><strong>Methods: </strong>This qualitative study was conducted in a regional referral hospital in eastern Uganda. Twelve midwives were recruited purposively at two different time points in December 2020 and February 2024. A data-driven reflexive thematic analysis was conducted.</p><p><strong>Results: </strong>Midwives' choice of labour positions was based on their understanding of the advantages and disadvantages for mothers and babies. Midwives were only open to changing their practices when they were confident in their knowledge and skills. The availability of resources influenced their perspectives on which positions were most feasible and effective in different situations. The midwives prioritised assessment of the mother's clinical condition rather than her preferences when choosing birth positions.</p><p><strong>Conclusion: </strong>This study highlighted midwives' willingness to support different and, to them, new labour positions when confident in their efficacy and safety. Addressing misconceptions about risks and equipment needs is therefore crucial. The prevailing provider-centric norms in hospitals may shape midwives' approach to care, highlighting the need for supportive environments to foster midwives' confidence in new practices.</p>","PeriodicalId":9033,"journal":{"name":"BMC Pregnancy and Childbirth","volume":"25 1","pages":"564"},"PeriodicalIF":2.8,"publicationDate":"2025-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12070630/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143953384","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Maternal plasma extracellular vesicles tsRNA as potential biomarkers for assessing preterm labor risk.","authors":"Xinrui Sun, Ying Feng, Liang Xie, Yilun Wu, Jia Mao, Yi Zhang, Linyu Zhang, Xin Yuan, Jiangping Ni, Xue Xiao, Hanmin Liu, Li Dai, Fang Ma","doi":"10.1186/s12884-025-07672-3","DOIUrl":"https://doi.org/10.1186/s12884-025-07672-3","url":null,"abstract":"<p><strong>Background: </strong>Spontaneous preterm labor (PTL) accounts for approximately 70% of preterm births, posing significant risks to both maternal and neonatal health. Current predictive biomarkers lack sufficient reliability, underscoring the need for non-invasive and dependable indicators. Emerging research indicates that tRNA-derived small RNAs (tsRNAs) are involved in various diseases; however, their potential association with PTL remains underexplored.</p><p><strong>Methods: </strong>Bioinformatics analyses of public GEO datasets (PRJNA415953 and PRJNA428989) were conducted to identify tsRNAs associated with PTL. Validation was performed using plasma extracellular vesicles samples collected at 12 weeks of gestation from PTL patients (n = 45) and healthy controls (n = 38). Functional assays were used to assess the impact of tsRNA1 (tRNA-Gly-GCC-5p-tRF-921) on extravillous trophoblast (EVT) function, including apoptosis, migration, invasion, and endothelial-like tube formation in HTR8/SVneo cells. Transcriptomic sequencing was conducted to identify tsRNA1-mediated pathways, and DNA methylation patterns were predicted based on the transcriptomic data. Statistical significance was determined using Student's t-test.</p><p><strong>Results: </strong>Two tsRNAs, tsRNA1 and tsRNA3 (tRNA-Gly-GCC-5p-tR-half-368), were significantly upregulated in PTL patient samples compared to controls. Overexpression of tsRNA1 impaired EVT function, increased apoptosis, and altered DNA methylation profiles, implicating its critical role in PTL mechanisms.</p><p><strong>Conclusions: </strong>This study identifies tsRNA1 as a key regulator of EVT dysfunction and placental pathology in PTL. The findings provide novel insights into the mechanistic role of tsRNAs in PTL and highlight tsRNA1 as a promising biomarker for early risk stratification and prediction of the condition.</p><p><strong>Clinical trial number: </strong>Not applicable.</p>","PeriodicalId":9033,"journal":{"name":"BMC Pregnancy and Childbirth","volume":"25 1","pages":"553"},"PeriodicalIF":2.8,"publicationDate":"2025-05-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12065249/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143962527","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Birthing partners - validation of a questionnaire to assess the well-being of support persons.","authors":"Nadine Schmitt, Juliane Lamprecht, Sabine Striebich, Gabriele Meyer","doi":"10.1186/s12884-025-07685-y","DOIUrl":"https://doi.org/10.1186/s12884-025-07685-y","url":null,"abstract":"<p><strong>Background: </strong>There is little research on the well-being of the persons giving support during childbirth and how they feel when doing this. The aim of this study is to validate a questionnaire that assesses the well-being of those support persons during childbirth. This publication focuses on the validation of the questionnaire; the development is described in more detail in a previous publication.</p><p><strong>Methods: </strong>After the questionnaire had been developed, it was sent online to a sample for validation. Subsequently, the internal consistency was determined to assess the reliability and the correlation with an external criterion in order to assess the criterion validity. Known-groups validation was used to assess the construct validity of the questionnaire. Linear regressions were carried out to analyse which variables influence well-being.</p><p><strong>Results: </strong>The results show good reliability and high criterion validity. The known-groups analyses identified group differences between the different birth modes regarding the different domains of well-being of the support persons. Factors influencing well-being are whether it is a first time or repeated support, whether the birthing woman is a primiparous or multiparous woman and whether the birth is vaginal or operative.</p><p><strong>Conclusion: </strong>Our results suggest that this questionnaire adequately captures important aspects of the well-being of support persons during childbirth. The questionnaire is designed for all birth experiences. In the case of negative experience it can be used to assess additional support and counselling and thus potentially promote the mental health of the support persons preventively.</p>","PeriodicalId":9033,"journal":{"name":"BMC Pregnancy and Childbirth","volume":"25 1","pages":"561"},"PeriodicalIF":2.8,"publicationDate":"2025-05-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12065179/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143978446","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}