BioMed Research International最新文献

{"title":"Correction to \"Pomegranate Seeds Extract Possesses a Protective Effect against Tramadol-Induced Testicular Toxicity in Experimental Rats\".","authors":"","doi":"10.1155/bmri/9862436","DOIUrl":"10.1155/bmri/9862436","url":null,"abstract":"<p><p>[This corrects the article DOI: 10.1155/2020/2732958.].</p>","PeriodicalId":9007,"journal":{"name":"BioMed Research International","volume":"2026 ","pages":"9862436"},"PeriodicalIF":2.9,"publicationDate":"2026-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12965108/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147376170","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Soil-Transmitted Helminthes (STHs) and Asymptomatic Bacteriuria Amongst Preschool Children in the Bosomtwe District of Ghana.","authors":"Kofi Agyapong Addo, Kwadwo Boampong, Samuel Ayetibo Ofori, Papa Kofi Amissah-Reynolds, Victor Agyei, John Asiedu Larbi","doi":"10.1155/bmri/9946374","DOIUrl":"10.1155/bmri/9946374","url":null,"abstract":"<p><p>Soil-transmitted helminths (STHs) and urinary tract infections (UTIs) pose major public health challenges, especially in regions with poor healthcare access, inadequate sanitation, and limited clean water supply. When these two conditions recur, they can cause stunted growth in children between 24 and 59 months of age, a vital phase for physical development. This study sought to assess the prevalence of STHs and asymptomatic bacteriuria among preschool children aged 1-5 years in the Bosomtwe District. A total of 344 children from 5 educational circuits were randomly selected for this study. Fecal specimens were obtained from each child and examined for STHs via the formol-ether concentration method, while urine samples were inoculated onto CLED agar to isolate and identify asymptomatic bacteriuria isolates. Antibiotic susceptibility was assessed using the Kirby-Bauer disk diffusion test. Participant demographics were obtained using a predesigned and structured survey questionnaire. The study found a prevalence of 19.8% (68/344) for STHs and 44.8% (154/344) for bacteriuria, with 6.1% (21/344) having both infections. <i>Ascaris lumbricoides</i> was the most common STH, accounting for 12.2% (42/344), followed by <i>Trichuris trichiura</i> 4.4% (15/344) and hookworm 3.2% (11/344). More males, 24.2% (40/165), than females, 15.6% (28/179), were affected by STHs. The Kuntenase circuit recorded the highest STH positivity with 26.1% (18/69). For bacteriuria, more females, 47.5% (85/179), than males, 41.8% (69/165) were affected, with age 5 being the most at-risk group, 46.6% (41/88). <i>Escherichia coli</i> was the most isolated Bacteriuria pathogen, 37.5% (129/344), followed by <i>Klebsiella</i> spp. 6.1% (21/344) and <i>Proteus</i> spp. 1.2% (4/344). Gentamicin was the most effective antibiotic against Bacteriuria isolates. Given the high prevalence of both STHs and bacteriuria, stakeholders should ensure better sanitation and health services, distribute anthelminthic drugs regularly, and raise awareness of bacteriuria in the district.</p>","PeriodicalId":9007,"journal":{"name":"BioMed Research International","volume":"2026 ","pages":"9946374"},"PeriodicalIF":2.9,"publicationDate":"2026-01-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12781086/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145950998","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Inflammatory Bowel Disease Outcomes in Northern Iran: A Retrospective Cohort Study of Remission, Complications, and Treatment Strategies.","authors":"Poria Hoseinialiabadi, Iradj Maleki, Hafez Fakheri, Mahboobe Ebrahimi, Elham Yousefi Abdolmaleki, Tarang Taghvaei, Hajar Shokri-Afra","doi":"10.1155/bmri/5363565","DOIUrl":"10.1155/bmri/5363565","url":null,"abstract":"<p><strong>Background: </strong>Inflammatory bowel disease (IBD), encompassing Crohn's disease (CD) and ulcerative colitis (UC), is a chronic inflammatory condition affecting the gastrointestinal tract. While significant progress has been made in managing IBD, the long-term outcomes remain heterogeneous, prompting this cohort study.</p><p><strong>Methods: </strong>A retrospective analysis was performed on patients with IBD registered between 2000 and 2023. Clinical data, including demographics, disease activity, remission rates, therapeutic approaches, and complications, were collected.</p><p><strong>Results: </strong>Among 600 enrolled patients, 72.8% were diagnosed with UC. Both patients with UC and those with CD showed improvements in endoscopic severity by the end of follow-up (<i>p</i> < 0.001). Clinical remission was achieved by 96.3% of patients with UC and 91.2% of those with CD during the last 6 months of follow-up (<i>p</i> = 0.046). Disease progression occurred in 22.1% of patients with CD and 18% of those with UC (<i>p</i> = 0.100). Gastrointestinal complications were more prevalent in patients with CD (25.6% vs. 1.6%, <i>p</i> < 0.001). Relapse remained high, with 86.7% of patients with UC and 92.6% of those with CD experiencing relapses at least once during follow-up, particularly those with severe baseline disease (<i>p</i> < 0.01). Biologic therapies were used more frequently (<i>p</i> < 0.01) and initiated earlier in patients with CD (<i>p</i> = 0.031).</p><p><strong>Conclusion: </strong>The study showed significant clinical improvement, yet many patients, particularly those with severe initial disease, had incomplete remission and frequent relapses, necessitating more effective long-term management strategies. The distinction between CD and UC highlights the need for specialized treatments.</p>","PeriodicalId":9007,"journal":{"name":"BioMed Research International","volume":"2026 ","pages":"5363565"},"PeriodicalIF":2.9,"publicationDate":"2026-01-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12780852/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145950992","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"XMP-Net: An XAI-Based Modified Xception Model for Recognizing Monkeypox and Other Skin Diseases.","authors":"Ferdib-Al-Islam, Prithvi Biswas, Partha Protim Gharami, Md Rahatul Islam","doi":"10.1155/bmri/1113178","DOIUrl":"10.1155/bmri/1113178","url":null,"abstract":"<p><p>This research introduces \"XMP-Net,\" a modified Xception-based deep learning architecture constructed for the categorization of skin conditions, with a particular focus on identifying monkeypox. The study recognizes skin images of four categories: normal, chickenpox, measles, and monkeypox. To enhance interpretability and foster confidence in the model's predictions, Grad-CAM (gradient-weighted class activation mapping) and LIME (local interpretable model-agnostic explanations) were employed to illustrate the model's thinking manner. The model demonstrated impressive classification performance, attaining an accuracy of 98.33% for normal skin, 98.25% for monkeypox, 84.21% for measles, and 77.27% for chickenpox. Precision, recall, and <i>F</i>1-score values were also analyzed for each class, with monkeypox achieving a precision of 91.80%, a recall of 98.25%, and an <i>F</i>1-score of 94.92%. The visual explanations generated by Grad-CAM and LIME highlighted critical parts in the input images that affected the model's likelihoods, offering clinicians valuable insights into the diagnostic process. This research underscores the potential of explainable artificial intelligence (XAI) in augmenting traditional diagnostic methods, particularly for emerging communicable maladies like monkeypox, and provides a foundation for developing reliable, interpretable, and accessible diagnostic tools for resource-constrained settings.</p>","PeriodicalId":9007,"journal":{"name":"BioMed Research International","volume":"2026 ","pages":"1113178"},"PeriodicalIF":2.9,"publicationDate":"2026-01-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12780539/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145951150","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hypertension and Its Determinants Among White-Collar Workers: A Cross-Sectional Study Focusing on Lifestyle Risk Factors and Health Literacy.","authors":"Sandeepa Karki, Shyam Sundar Budhathoki, Shishir Paudel, Dhurba Khatri, Anup Ghimire, Deepak Kumar Yadav, Paras Kumar Pokharel","doi":"10.1155/bmri/3545756","DOIUrl":"10.1155/bmri/3545756","url":null,"abstract":"<p><strong>Background: </strong>Hypertension is a growing public health concern, particularly among white-collar workers exposed to sedentary lifestyles and occupational stress. This study examines the prevalence and determinants of hypertension among commercial bank employees in Sunsari, Nepal, with a focus on lifestyle risk factors and health literacy.</p><p><strong>Methods: </strong>A cross-sectional study was conducted among 240 bank employees using stratified random sampling. Data were collected through a self-administered questionnaire adapted from the WHO STEPS survey and clinical measurements of blood pressure, BMI, and waist circumference. Hypertension was classified based on the JNC 8 guidelines. Descriptive statistics, chi-square tests, and independent <i>t</i> tests were used for preliminary analysis. Logistic regression was performed to identify independent predictors of hypertension, controlling for potential confounders.</p><p><strong>Results: </strong>The prevalence of hypertension was 62.1% (95% CI 58.6-65.6), with 47.5% in Stage I and 14.6% in Stage II. Males had significantly higher odds of hypertension than females (aOR 2.237, 95% CI 1.170-4.276). Behavioral risk factors such as alcohol consumption (aOR 4.732, 95% CI 1.386-16.160) and frequent processed food intake (aOR 2.640, 95% CI 1.024-7.096) were significantly associated with hypertension. Overweight (aOR 1.819) and obesity (aOR 1.575) were also found to be the major risk factors. Lower health literacy scores, particularly in healthcare engagement and self-management, were associated with hypertension.</p><p><strong>Conclusion: </strong>The high prevalence of hypertension among bank employees highlights the need for workplace interventions promoting healthy lifestyles, routine screenings, and health literacy programs to improve hypertension awareness and management.</p>","PeriodicalId":9007,"journal":{"name":"BioMed Research International","volume":"2026 ","pages":"3545756"},"PeriodicalIF":2.9,"publicationDate":"2026-01-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12780851/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145950953","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Heart Rate Variability-Derived Decision Support Tool for Prognostication in Emergency Department Patients With Suspected Infection.","authors":"Andrew J E Seely, Douglas P Barnaby, Natasha Hudek, Christophe L Herry, Nathan B Scales, Shannon M Fernando, Jamie C Brehaut, Jeffrey J Perry","doi":"10.1155/bmri/3778740","DOIUrl":"10.1155/bmri/3778740","url":null,"abstract":"<p><strong>Aims: </strong>Prediction of future deterioration in emergency department patients with infection is difficult, and existing prognostic tools are inaccurate. We evaluated the feasibility of deployment of a clinical decision support tool, Sepsis Advisor, which utilizes heart rate variability and laboratory values to predict future deterioration in emergency department patients with treated infection.</p><p><strong>Methods: </strong>This study was an observational, prospective, Pilot Phase 1 feasibility implementation study involving two sites within a single academic health sciences centre. Then, 71 patients were enrolled, all with suspected/treated infection and systemic inflammatory response. Patients underwent 30 min of electrocardiograph recording. The generated predictive model and Sepsis Advisor report were shown to physicians observationally, > 48 h after clinical encounter, while assessing perceived usability, value, barriers and drivers with using the tool through interviews with nurses and physicians.</p><p><strong>Results: </strong>Of the 71 patients enrolled, 65 (92%) had adequate duration of heart rate variability measurements to generate a predictive model (average recording: 25 ± 7 min); 100% had clinical data entry. Creatinine, lactate, and INR were drawn 97%, 56%, and 28% of the time and were incorporated into predictive models. Physician and nurse reported drivers for use included potential to facilitate communication, improve care, and ease of integration. Barriers included the need to understand and interpret results from the tool, time constraints, changing routines, and gaining buy-in. User-centered feedback informed four improved versions of the tool.</p><p><strong>Conclusions: </strong>Observational deployment of a heart rate variability-based clinical decision support tool within the emergency department is feasible and perceived to have the potential to improve care.</p>","PeriodicalId":9007,"journal":{"name":"BioMed Research International","volume":"2026 ","pages":"3778740"},"PeriodicalIF":2.9,"publicationDate":"2026-01-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12781178/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145951328","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Quantitative Treatments for Explaining the Mechanism and Kinetics of Catalytic Electron Transfers in Murburn Processes, Particularly Involving Heme Enzymes Like (Per)oxidases and P450s.","authors":"Kelath Murali Manoj, Daniel Andrew Gideon, Philip Moses Samuel, Suhotra Das","doi":"10.1155/bmri/3079294","DOIUrl":"10.1155/bmri/3079294","url":null,"abstract":"<p><p>The seminal Michaelis-Menten theorization for biological catalysis was based on \"transition state\" (TS), involving the formation of a topologically complementary substrate (S) and enzyme (E) complex (ES) at the \"active site\" of the latter. Rudolph Marcus put forth the theory of outer sphere electron transfer (ET) in a \"donor-acceptor\" TS complex, which was seen as a foundational framework for understanding ET reactions in chemical systems. Although these two theories are quite robust, the active site treatment of Michaelis-Menten may not be relevant in promiscuous/nonspecific xenobiotic-metabolizing redox enzymes, and Marcus theory's applicability to biological ET (BET) systems can be limited in interfacial protein-protein interactions. Herein, the \"mathematical\" necessity to venture beyond the \"active site constraints\" of interpreting redox enzyme kinetics and BETs is established first with fresh data. Also, (i) the classical explanation vouching for active site binding and protein-protein complexation-based BET in xenobiotic metabolism (mediated at the endoplasmic reticulum membranes of hepatocytes) and oxidative phosphorylation (multiprotein machinery at mitochondrial cristae) is demonstrated to be untenable, and (ii) tangible/viable murburn models were proposed in lieu. Therefore, toward the imperative goal of arriving at quantitative expressions correlating the parameters/variables involved, the foundational considerations of murburn ET and murzyme catalysis in simple heme systems are presented, with some assumptions/constraints. While some derivations are from ab initio considerations, others are heuristic/empirical, often needing experimental fitting. The linear time-course profiles of ET (substrate depletion) and the biphasic substrate-dependent (product formation) are well fit with the newly derived expressions. A mechanistic comparison of the murburn model vis-à-vis the longstanding P450cam explanation for drug/xenobiotic metabolism is also provided.</p>","PeriodicalId":9007,"journal":{"name":"BioMed Research International","volume":"2026 ","pages":"3079294"},"PeriodicalIF":2.9,"publicationDate":"2026-01-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12780854/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145950983","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Condylar Bone Quality in Growing Children Is Associated With Genetic Polymorphisms in Genes Involved in Calcium and Phosphate Maintenance.","authors":"Erika Calvano Küchler, Caio Luiz Bitencourt Reis, Gabriela Fonseca-Souza, Daniel Hemming, Flares Baratto-Filho, Cristiano Miranda de Araujo, Svenja Beisel-Memmert, Juliana Feltrin-Souza, Michelle Nascimento Meger, Bianca Lopes Cavalcante-Leão","doi":"10.1155/bmri/9337029","DOIUrl":"10.1155/bmri/9337029","url":null,"abstract":"<p><p>Single nucleotide polymorphisms (SNPs) play a crucial role in regulating vitamin D, parathyroid hormone (PTH), and calcitonin concentrations, which are involved in bone health. Some reports suggested that fractal analysis is useful in the morphometric analysis of the mandible trabecular bone in panoramic radiographs. Therefore, we investigated if SNPs in genes that influence vitamin D, calcitonin, and PTH levels are involved in condylar bone quality during the active growing phase of the mandible. Fractal dimension was obtained from the condyle region of interest (ROI) using panoramic radiographs and used to measure the complexity and the microarchitecture of the bone. Fractal dimension using the box-counting algorithm was then calculated. In order to avoid information bias, a script to automate the commands in the software ImageJ was generated to ensure consistency and minimize the potential for human error during the data analysis process. SNPs in vitamin D receptor (<i>VDR</i>), cytochrome P450 family 27 subfamily B member 1 (<i>CYP27B1</i>), cytochrome P450 family 24 subfamily A member 1 (<i>CYP24A1</i>), vitamin D binding protein (<i>VDBP</i>), SEC23 homolog A (<i>SEC23A</i>), calcitonin receptor (<i>CALCR</i>), and parathyroid hormone (<i>PTH</i>) were analyzed. DNA extracted from saliva was used for genotyping analysis of <i>VDR</i> (rs7975232, rs2228570, and rs1544410), <i>CYP27B1</i> (rs4646536), <i>CYP24A1</i> (rs927650), <i>VDBP</i> (rs4588), <i>SEC23A</i> (rs8018720), <i>CALCR</i> (rs1801197), and <i>PTH</i> (rs6256, rs307247, and rs694). A statistical analysis was performed with an alpha error tolerance of 5%. A total of 100 children were included; 50 (50%) were boys and the age ranged from 5 to 14 years old. Fractal dimensions were compared among genotypes. The GT (mean = 1.20 and standard error = 0.03, <i>p</i> = 0.024) and TT genotypes (mean = 1.16 and standard error = 0.06, <i>p</i> = 0.047) in the gene VDBP (rs4588) presented lower fractal dimension. The GG genotype in SEC23A (rs8018720) (mean = 1.34 and standard error = 0.03, <i>p</i> = 0.011) and the TC genotype in PTH (rs694) showed an increased fractal dimension (mean = 1.29 and standard error = 0.03, <i>p</i> = 0.020). In conclusion, SNPs in VDBP, SEC23A, and PTH encoding genes are associated with mandibular condylar trabecular bone structure in children.</p>","PeriodicalId":9007,"journal":{"name":"BioMed Research International","volume":"2026 ","pages":"9337029"},"PeriodicalIF":2.9,"publicationDate":"2026-01-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12776005/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145931580","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Factors Associated With <i>Toxoplasma gondii</i> Infection Among Pregnant Women Admitted for Delivery in Selected Hospitals in Dar es Salaam, Tanzania.","authors":"Masauko Liponda, Secilia Ng Weshemi, David Munisi","doi":"10.1155/bmri/8584162","DOIUrl":"10.1155/bmri/8584162","url":null,"abstract":"<p><p>Toxoplasmosis is asymptomatic in infected individuals; however, infections acquired during pregnancy pose a significant risk of the development of congenital parasitosis, leading to poor pregnancy outcomes and various degrees of chorioretinitis later in life. This study was therefore designed to determine the seroprevalence of <i>Toxoplasma gondii</i> and its associated factors among pregnant women admitted for delivery in selected hospitals in Dar es Salaam. A hospital-based analytical cross-sectional study was conducted among 191 pregnant women who were admitted for delivery in the selected hospitals in Dar es Salaam, Tanzania. A pretested, Kiswahili-translated semistructured questionnaire was used to collect demographic characteristics of the respondents, as well as their risk profile and awareness on <i>T. gondii</i>. Collected blood samples were screened for <i>T. gondii</i> IgG and IgM using the FaStep TORCH IgG/IgM rapid test device as per the manufacturer's instructions. Data analysis was done using STATA Version 14 (StataCorp, Texas, United States); figures were plotted using Microsoft Excel [2024] (Microsoft Corporation, Redmond, Washington, United States). Crude and adjusted OR were estimated by bivariable and multivariable logistic regression analysis with respective 95% CIs, respectively. A <i>p</i> value less than or equal to 0.05 was considered statistically significant. The seroprevalence of <i>T. gondii</i> among study participants was found to be 20.94%. Multivariable logistic regression for factors associated with <i>T. gondii</i> serostatus was modeled. After controlling for other factors, consumption of rodents for food (<i>A</i> <i>O</i> <i>R</i> = 10.52, 95% <i>C</i> <i>I</i> = 1.13-97.79, <i>p</i> = 0.039) and consumption of game meat (<i>A</i> <i>O</i> <i>R</i> = 3.84, 95% <i>C</i> <i>I</i> = 1.56-9.46, <i>p</i> = 0.003) were the best predictors of <i>T. gondii</i> seropositivity. Consumption of untreated water for drinking among study participants was found to be very high, although it was not associated with <i>T. gondii</i> seropositivity. This calls for the need for implementation of public health education with a particular emphasis on proper cooking of game meat and rodents before consumption and proper treatment of drinking water.</p>","PeriodicalId":9007,"journal":{"name":"BioMed Research International","volume":"2026 ","pages":"8584162"},"PeriodicalIF":2.9,"publicationDate":"2026-01-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12766278/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145910376","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unraveling the Causal Linkages of <i>RBP7</i> and <i>SCGB3A1</i> on Pelvic Organ Prolapse: Multifaceted Insights From Genome-Wide Mendelian Randomization, Single-Cell RNA Analysis, and Network Pharmacology.","authors":"Ying Yang, Weiyuan Xing, Xiaoqin Wang, Qiran Sun, Ningning Hu, Liwen Zhang, Fuyun Dong, Rujun Chen","doi":"10.1155/bmri/9785848","DOIUrl":"10.1155/bmri/9785848","url":null,"abstract":"<p><strong>Background: </strong>Pelvic organ prolapse (POP) is a common pelvic floor disorder in middle-aged and elderly women. Its pathophysiology is complex, involving weakened pelvic floor muscles and connective tissues. There is a need to explore its underlying pathogenesis and develop effective treatments.</p><p><strong>Methods: </strong>We integrated single-cell sequencing (scRNA-seq) data analysis with Mendelian randomization (MR) analysis. scRNA-seq data of vaginal mucosal tissue were obtained from individuals with and without pelvic organ prolapse (POP); we then performed dimensionality reduction and cell subset identification. MR was conducted using GWAS summary statistics and eQTL data, following STROBE-MR guidelines. We also performed protein-protein interaction analysis, functional enrichment analysis, drug prediction, and molecular docking.</p><p><strong>Results: </strong>We identified <i>RBP7</i> as a POP risk factor and <i>SCGB3A1</i> as a protective factor. <i>RBP7</i> high expression increased POP risk (IVW, OR 1.262, 95% CI 1.093-1.459, <i>p</i> = 0.002), whereas <i>SCGB3A1</i> high expression decreased it (IVW, OR 0.907, 95% CI 0.844-0.975, <i>p</i> = 0.008). We found associated key genes and their biological processes and signaling pathways. We also predicted potential drugs and their binding affinities.</p><p><strong>Conclusion: </strong>The study highlights the significance of fibroblast gene expression changes in collagen metabolism for POP. It identified risk and protective genes and explored potential drugs. Future research should verify <i>SCGB3A1</i> functions in fibroblasts, conduct preclinical drug trials, and clarify POP molecular mechanisms.</p>","PeriodicalId":9007,"journal":{"name":"BioMed Research International","volume":"2026 ","pages":"9785848"},"PeriodicalIF":2.9,"publicationDate":"2026-01-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12765987/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145910289","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}