BioMed Research International最新文献

{"title":"Transcriptome Analysis of Platelet-Rich Plasma-Treated Osteoarthritic Chondrocyte.","authors":"Dae Keun Suh, Woo Jin Yeo, Kuhoang Cheong, Jae-Won Heo, Dong Hyeon Kim, Soo Mi Lee, Yong-Soo Lee, Dong Won Suh","doi":"10.1155/2024/7680736","DOIUrl":"10.1155/2024/7680736","url":null,"abstract":"<p><p>As a blood-derived biomaterial, platelet-rich plasma (PRP) has been considered a potential therapy and tried in knee and hip osteoarthritis with beneficial effects as an anti-inflammatory and potent regenerative agent. To better understand the substantial effect of PRP on chondrocytes in an inflammatory environment, we analyzed the transcriptome profile by RNA sequencing (RNA-seq) after PRP administration in IL-1<i>β</i>-treated osteoarthritic chondrocytes which were isolated from human knee articular cartilage tissue. A total of 24,424 genes were analyzed, and significant changes in expression were observed for 226 genes in the control (CTL) versus IL-1<i>β</i> group and 300 genes in the IL-1<i>β</i> versus IL-1<i>β</i>+PRP group. The Top 20 significantly upregulated and downregulated genes and the major altered genes in nine categories that are closely related to chondrocyte physiology were analyzed, and the expression of several important genes in each category was evaluated by qRT-PCR and western blot analysis. Our study revealed that the PRP, at the gene expression level, has apparent anti-inflammatory, cell proliferative, and regenerative activities in chondrocytes in the presence of IL-1<i>β</i>, which mimic an osteoarthritic environment. Identifying potent molecules that regulate cartilage physiology represents a promising therapeutic approach for suppressing cartilage degeneration, especially that caused by inflammation-induced osteoarthritis.</p>","PeriodicalId":9007,"journal":{"name":"BioMed Research International","volume":"2024 ","pages":"7680736"},"PeriodicalIF":2.6,"publicationDate":"2024-11-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11604281/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142749565","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Protective Action of <i>Coutarea hexandra</i> (Rubiaceae) on the Neuromuscular Blockade Induced by <i>Lachesis muta muta</i> (Viperidae: Crotalinae) Venom.","authors":"Grazielle D Pilon, Anna P Farias-de-França, Nathalia M Cantuária, Magali G Silva, Aline G Leão-Torres, Rafael S Floriano, Marcio G Dos Santos, Nelson Jorge da Silva, Otto M S Gerlach, Valdir Cechinel-Filho, Yoko Oshima-Franco","doi":"10.1155/2024/4714510","DOIUrl":"10.1155/2024/4714510","url":null,"abstract":"<p><p>Envenomations by snakes represent a neglected health problem in tropical and subtropical countries. In South America, <i>Lachesis muta</i> occasionally causes severe human envenomation, with treatment being conditioned to an unspecific antivenom. In this work, we examined the neutralizing ability of <i>Coutarea hexandra</i> stem bark hydroalcoholic extract (<i>Ch</i>-E), including the commercial phytochemicals coumarin and quinine, on the neuromuscular blockade induced by <i>L. m. muta</i> venom in mouse phrenic nerve-diaphragm preparation. Biological assays were performed following conventional myographic technique ex vivo. <i>Ch</i>-E was phytochemically characterized to detect the presence of coumarin and quinine using analytical methods. <i>Ch</i>-E and commercial phytochemicals were tested separately or combined under pre- and post-venom incubation protocols. <i>Ch</i>-E attenuated the venom-induced neuromuscular blockade only under the pre-venom incubation protocol. Quinine was not detected in <i>Ch</i>-E. Commercial coumarin and quinine exhibited a concentration-dependent counteracting effect on the venom-induced neuromuscular blockade. The pre-venom incubation protocol showed to be efficient in attenuating the <i>L. m. muta</i> venom-induced neuromuscular blockade, most likely due to the presence of coumarin derivatives and unknown alkaloids in this extract.</p>","PeriodicalId":9007,"journal":{"name":"BioMed Research International","volume":"2024 ","pages":"4714510"},"PeriodicalIF":2.6,"publicationDate":"2024-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11584258/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142709063","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unveiling the Link Between Breast Cancer Treatment and Osteoporosis: Implications for Anticancer Therapy and Bone Health.","authors":"Bhawna Goel, Tarun Virmani, Vikas Jain, Girish Kumar, Ashwani Sharma, Abdullah Al Noman","doi":"10.1155/2024/5594542","DOIUrl":"10.1155/2024/5594542","url":null,"abstract":"<p><p><b>Background:</b> The interplay between breast cancer treatment and osteoporosis has important consequences for anticancer therapy and patient bone health. Many breast cancer therapies involve hormonal treatments that lower estrogen levels, which can lead to an increased risk of osteoporosis due to reduced bone mineral density. Aromatase inhibitors, chemotherapy, and surgeries such as oophorectomy can further aggravate bone loss, highlighting the necessity of prioritizing bone health during cancer treatment. <b>Objective:</b> This review is aimed at investigating the complex relationship between breast cancer therapies and bone health by examining the molecular and cellular mechanisms through which anticancer treatments lead to bone loss. It also seeks to assess the effects of various treatment options, such as selective estrogen receptor modulators (SERMs) and bisphosphonates, on reducing bone loss and maintaining bone health during cancer therapy. <b>Method:</b> The review explores the mechanisms underlying bone loss in breast cancer patients undergoing treatment, focusing on factors such as estrogen depletion, inflammatory cytokines, and changes in bone remodelling processes. Additionally, it evaluates the efficacy of different therapeutic interventions, including pharmacological treatments like bisphosphonates and third-generation SERMs, in mitigating bone-related side effects. <b>Results:</b> The findings indicate a critical need to balance the effectiveness of breast cancer treatments with the preservation of bone health. Pharmacological treatments like bisphosphonates and denosumab have been identified as essential for managing bone health in breast cancer patients. Furthermore, third-generation SERMs show potential in reducing bone loss associated with cancer therapy.</p>","PeriodicalId":9007,"journal":{"name":"BioMed Research International","volume":"2024 ","pages":"5594542"},"PeriodicalIF":2.6,"publicationDate":"2024-11-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11581800/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142685915","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Patterns, Potential Teratogenicity, and Associated Factors of Drugs Prescribed to Pregnant Women Attending Antenatal Care Units in Debre Tabor Comprehensive Specialized Hospital, Debre Tabor, Northwest Ethiopia.","authors":"Muluken Adela Alemu, Woretaw Sisay Zewdu, Yared Andargie Ferede, Mulugeta Molla Zeleke, Teklie Mengie Ayele, Abraham Nigussie Assefa, Tirsit Ketsela Zeleke, Achenef Bogale Kassie","doi":"10.1155/2024/5577862","DOIUrl":"10.1155/2024/5577862","url":null,"abstract":"<p><p><b>Background:</b> About 80% of pregnant women use at least one medication during their pregnancy period. Many drugs that are not allowed to be used during pregnancy (from FDA Pregnancy Categories D and X) were used. Irrational use of these drugs during pregnancy may result in different birth defects, as explained by thalidomide and diethylstilbestrol's tragedy. Knowledge of drug utilization and associated factors that affect the pattern is important to enhance rational prescribing. But information about prescription patterns and associated factors among pregnant women is scarce in the Debre Tabor area and generally in Ethiopia. <b>Objective:</b> This study was aimed at assessing drug prescription patterns, potential teratogenicity, and associated factors among pregnant women attending the antenatal care unit at Debre Tabor Comprehensive Specialized Hospital, Debre Tabor, Northwest Ethiopia. <b>Methods:</b> A retrospective cross-sectional study design was performed on 359 pregnant women attending antenatal care units from June 01, 2022, to August 30, 2022, in the hospital. Necessary data were obtained through a questionnaire by reviewing the medical charts of the women. Analysis of the data was performed using SPSS Version 23. The association of the independent variables to medication use was assessed using multivariate logistic regression. A <i>p</i> value of less than 0.05 was considered significant. <b>Results:</b> Most of the study participants (325/359) were married (90.5%). From a total of 359 participants, 350 (97.5%) were prescribed with drugs. About 64% (385/602) of the prescribed medications were iron and vitamins. The most commonly prescribed medications are iron and folic acid combination (340/602, 56.5%), albendazole (48/602, 8%), mebendazole (37/602, 6.1%), omeprazole (33/602, 5.5%), followed by amoxicillin (32/602, 5.3%). The majority (79.3%) of the drugs were from FDA Pregnancy Categories A and B. Prescribed drug utilization was more probable in women who first visited the facility at their second (AOR = 2.91, 95% CI [1.12-6.64]) and third trimesters (AOR = 4.32, 95% CI [1.37-6.81]), had chronic illness (AOR = 7.54, 95% CI [2.34-14.68]), and live in rural areas (AOR = 2.47, 95% CI [1.56-8.43]). <b>Conclusion:</b> The study revealed that the prescription pattern in the hospital is in line with the WHO reference. Age, gravidity, number of ANC visits, first visit to the facility, presence of chronic illness, educational status, and residency were significantly associated with prescription drug use in pregnant mothers. But still, some pregnant women received drugs that may have teratogenicity risk (FDA Category C).</p>","PeriodicalId":9007,"journal":{"name":"BioMed Research International","volume":"2024 ","pages":"5577862"},"PeriodicalIF":2.6,"publicationDate":"2024-11-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11573445/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142667406","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Role of Genetically Modified Microorganisms for Effective Elimination of Heavy Metals.","authors":"Shashi Kiran Misra, Ajay Kumar, Kamla Pathak, Girish Kumar, Tarun Virmani","doi":"10.1155/2024/9582237","DOIUrl":"10.1155/2024/9582237","url":null,"abstract":"<p><p>Heavy metals are lethal and hazardous pollutants for the ecosystem owing to their virtues including acute toxicity, prolonged persistence, and bioaccumulation. These contaminants are not only a threat to aquatic/terrestrial biota but also pose serious health issues to humans. Natural and anthropologic processes consistently upsurge heavy metal concentration beyond acceptable limits and mobilization and hence disturb biogeochemical cycles and the food chain, although several conventional strategies including adsorption, chemical precipitation, ion exchange, and membrane separation methods are being employed for the removal of these lethal heavy metals from the ecosystem but failed due to lower efficiency rates and high application charges. The current scenario highly demands advanced biosorption or bioaccumulation processes that slow down heavy metal mobilization within the acceptable limit in the ecosystem. Genetically modified microorganisms (GMMs) with desired features are developed through interdisciplinary participation of genomics, molecular microbiology, and bioinformatics that have more potential to bioremediate heavy metals than the native microbes from polluted ecosystems. The study focuses on different sources of heavy metals, their impact on the ecosystem, and the bioremediation of toxic heavy metals via GMMs.</p>","PeriodicalId":9007,"journal":{"name":"BioMed Research International","volume":"2024 ","pages":"9582237"},"PeriodicalIF":2.6,"publicationDate":"2024-11-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11568892/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142646983","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Examining Cesarean Section Rates in Ghana's 10 Regions Over a Decade a Comprehensive National Investigation.","authors":"Senyefia Bosson-Amedenu, Abdulzeid Anafo, Ahmed Ouerfelli, Nabil Ouerfelli, Noureddine Ouerfelli","doi":"10.1155/2024/3774435","DOIUrl":"10.1155/2024/3774435","url":null,"abstract":"<p><p>This study examines cesarean section (C-section) deliveries in Ghana from 2008 to 2017 in 10 regions, distinguishing between scheduled and emergency procedures. Scheduled C-sections target specific maternal conditions, such as advanced age, multiparity, and medical history, while emergency C-sections address acute fetal distress, preeclampsia, bleeding, and other urgent situations. The analysis reveals various regional patterns, with the Brong-Ahafo Region showing a potential deceleration after 2017 and the Upper West Region indicating a possible acceleration. The high number of C-sections in Greater Accra and Ashanti may be related to population density and health facilities. The study proposes empirical models, including linear, quadratic, and exponential components, emphasizing quasilinearity. The exponential model suggests transient and permanent phases of cesarean frequency, with the latter dominated by quasilinearity. Optimal parameter values are determined, which highlights the stability of the model. However, caution is advised when projecting too far into the future due to the inevitable slowing of observed trends. The findings offer insights for healthcare planning, resource allocation, and policymaking, emphasizing the need for region-specific approaches and ongoing monitoring of cesarean dynamics to inform nuanced interventions.</p>","PeriodicalId":9007,"journal":{"name":"BioMed Research International","volume":"2024 ","pages":"3774435"},"PeriodicalIF":2.6,"publicationDate":"2024-11-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11568890/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142646982","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prevalence of Women's Home Birth Preferences and Its Associated Factors in Ethiopia: A Systematic Review and Meta-Analysis.","authors":"Temesgen Geta, Dereje Haile, Abiy Girma","doi":"10.1155/2024/5780900","DOIUrl":"10.1155/2024/5780900","url":null,"abstract":"<p><p><b>Background:</b> In low-income countries, such as Ethiopia, home birth is the main cause of maternal and neonatal mortality. Several separate studies have been conducted on the prevalence of home birth preference. However, there is no pooled prevalence of home birth preferences. So, this systematic review and meta-analysis is aimed at assessing the overall preference for home birth and related factors among Ethiopian women. <b>Methods and Materials:</b> The review included only published articles. Medline/PubMed, Web Science, Google Scholar, Scopus, and the Cochrane Library are the main databases. The review includes cross-sectional studies in English that meet eligibility requirements. The combined prevalence of women's preference for home birth is calculated by random effect models. In addition, Egger's tests and funnel diagrams were used to investigate publication biases. STATA Version 14 is used to perform all statistical analyses. <b>Results:</b> The review included 14 studies with 6631 participants. In Ethiopia, the prevalence of women's preference for home birth was 41.48% (confidence interval (CI): 49.99; 63, 56; I2:98.7%; <i>p</i> ≤ 0.001). In the analysis of the subgroups, the Oromia region had the highest home birth preference rate at 61.40% (55.54%, 67.16), while southern Ethiopia had the lowest value at 20.52% (5.18, 29.75). The probability of preferring home birth was higher for women without education (OR = 0.22, <i>p</i> ≤ 0.001, <i>I</i> ² = 69.7%) and for younger women (ODR = 0.47, <i>p</i> ≤ 0.001, <i>I</i> ² = 84.2%). <b>Conclusion:</b> According to the study, 41% of Ethiopian women prefer home births over institutional births. Age and education of women are statistically important factors in the choice of birthplace. To solve this problem, health professionals and other stakeholders are strongly encouraged to provide women's health education at the community and institutional levels.</p>","PeriodicalId":9007,"journal":{"name":"BioMed Research International","volume":"2024 ","pages":"5780900"},"PeriodicalIF":2.6,"publicationDate":"2024-11-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11561181/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142614121","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Multicentre Cross-Sectional Study on Hepatitis B Vaccination Coverage and Associated Factors Among Personnel Working in Health Facilities in Kumasi, Ghana.","authors":"Daniel Kobina Okwan, Godfred Yawson Scott, Pius Takyi, Clinton Owusu Boateng, Philemon Boasiako Antwi, Akwasi Amponsah Abrampah, De-Graft Kwaku Ofosu Boateng, Michael Agyemang Obeng","doi":"10.1155/2024/8899638","DOIUrl":"10.1155/2024/8899638","url":null,"abstract":"<p><p><b>Background:</b> As part of efforts to reach the elimination target by 2030, the WHO and CDC recommend that all HCWs adhere to the three-dose Hepatitis B vaccination schedule to protect themselves against the infection. This study assessed Hepatitis B vaccination coverage and associated factors among personnel working in health facilities in Kumasi, Ashanti Region, Ghana. <b>Materials and Methods:</b> A cross-sectional study involving 530 HCWs was conducted in four hospitals in Kumasi from September to November 2023. An investigator-administered questionnaire was employed in gathering participant demographics and other information related to vaccination coverage. IBM SPSS Version 26.0 and GraphPad Prism 8.0 were used for analysing the data. <b>Results:</b> Even though the majority (70.6%) reported having taken at least one dose of the vaccine, only 43.6% were fully vaccinated (≥ 3 doses). More than a quarter (29.4%) had not taken any dose of the HBV vaccine. Close to a quarter (23.6%) had not screened or tested for HBV infection in their lifetime. The statistically significant variables influencing vaccination status were age, marital status, profession, and status in the hospital. Nearly one-half (44.9%) of the participants who have not taken the vaccine reported they do not have a reason for not taking it, and a high proportion (80.1%) were willing to take the vaccine when given for free. <b>Conclusion:</b> To combat the low Hepatitis B vaccination coverage among healthcare workers in Kumasi, Ghana, amidst the significant public health threat of HBV infection, comprehensive measures are necessary. These include implementing infection prevention control programmes, enhancing occupational health and safety, and conducting health promotion campaigns in healthcare facilities. Extending and intensifying Hepatitis B screening and vaccination initiatives to tertiary institutions and encouraging employers, supervisors, or team leaders to provide these services nationwide are also recommended.</p>","PeriodicalId":9007,"journal":{"name":"BioMed Research International","volume":"2024 ","pages":"8899638"},"PeriodicalIF":2.6,"publicationDate":"2024-11-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11557171/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142614120","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Self-Management and Its Associated Factors Among People Living With HIV at University of Gondar Comprehensive Specialized Hospital: A Cross-Sectional Study.","authors":"Abdisa Gemedi Jara, Masho Tigabe Tekle, Faisel Dula Sema, Banchamlak Teferi Mekonen, Asrat Elias Ergena, Amensisa Hailu Tesfaye, Saron Naji Gebremariam, Rahel Belete Abebe, Eyayaw Ashete Belachew, Abenezer Melaku Tafese, Eden Abetu Mehari","doi":"10.1155/2024/5590331","DOIUrl":"https://doi.org/10.1155/2024/5590331","url":null,"abstract":"<p><p><b>Background:</b> Self-management (SM) is the gold standard of care and is the direct active participation of patients in their disease management. Condition-specific factors, physical and social environment, individual and family, and the process of SM are factors that influence SM in people living with human immunodeficiency virus (PLHIV). Poor SM leads to high retroviral infection transmission, mortality, and morbidity. <b>Objective:</b> This study was aimed at assessing SM and its associated factors among PLHIV at the University of Gondar Comprehensive Specialized Hospital (UOGCSH), Northwest Ethiopia. <b>Methods:</b> A cross-sectional study was conducted using a systematic random sampling technique at the UOGCSH from May 20 to July 30, 2022. The data were collected using a previously validated tool and were entered and analyzed using Statistical Package for Social Sciences Version 25. A binary logistic regression analysis was used to identify factors associated with poor SM. The statistical significance was considered at a <i>p</i> value < 0.05. <b>Result:</b> Of 419 PLHIV, the median (IQR) SM score was 39 (9), and above half (52.6%, 95% CI: 48%-57%) of them had poor SM. Being unemployed (AOR = 2.49, 95%CI = 1.19, 5.19), living alone (AOR = 2.16, 95%CI = 1.12, 4.17), unfamiliar with the management of HIV-related symptoms (AOR = 3.59, 95%CI = 2.08, 6.20), poor social support (AOR = 3.02, 95%CI = 1.54, 5.93), poor self-efficacy (AOR = 3.04, 95%CI = 1.83, 5.06), and unsupported by the adherence support group (AOR = 17.17, 95%CI = 8.37, 35.22) were significantly associated with poor SM. <b>Conclusion:</b> The majority of PLHIV had poor SM. This study supports the findings of individual family SM theory and previously published studies regarding factors affecting SM. The government, hospital, adherence support groups, and PLHIV should work on modifiable sociodemographic, condition-specific, and process of SM to improve SM of PLHIV.</p>","PeriodicalId":9007,"journal":{"name":"BioMed Research International","volume":"2024 ","pages":"5590331"},"PeriodicalIF":2.6,"publicationDate":"2024-11-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11557183/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142614143","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Anticancer and Antioxidant Properties of <i>Vernonia amygdalina</i> Delile and <i>Citrus aurantifolia</i> (Christm.) Swingle Juice Extracts: An In Vitro Study.","authors":"Eunice E Ampem Danso, Eunice Dotse, Abigail Aning, Trudy Philips, Sherif Hamidu, Janet Ampofo","doi":"10.1155/2024/9692656","DOIUrl":"https://doi.org/10.1155/2024/9692656","url":null,"abstract":"<p><p><b>Introduction:</b> <i>Vernonia amygdalina</i> Delile (VAD), also known as bitter leaf, is widely utilized in traditional medicine for the treatment of various ailments, including cancer. The presence of bioactive compounds in VAD is believed to be responsible for its characteristic bitterness. In Ghana, it is a common practice to mitigate the bitterness of VAD by combining it with <i>Citrus aurantifolia</i> (Christm.) Swingle (lime) juice extracts, although this method lacks scientific evidence and documentation. Therefore, the antioxidant and anticancer activities of VAD and lime juice extracts (V5) and their combined effects were evaluated in vitro. <b>Method:</b> The antioxidant activity and cytotoxic effects of VAD extracts were determined against Jurkat, MCF-7, HepG2, and PNT2 cells using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay to quantify antioxidant activity and the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay to assess cytotoxicity. The statistical analysis of the data was conducted using Microsoft Excel and GraphPad Prism 8.0. Linear regression was employed to determine the correlation between the concentration and the percentage of antioxidant activity, while <i>p</i> values were calculated using Student's <i>t</i>-test. <b>Results:</b> The laboratory analysis focused on the extracts V1, V2, V3, V4, and V5. Briefly, V1 and V2 contained equal amounts of saponins and terpenoids. Among these, V2 exhibited the highest free radical scavenging activity, as indicated by an EC50 value of 2.14 ± 0.06 mg/mL. V2 also demonstrated cytotoxicity against the MCF-7, HepG2, Jurkat, and PNT2 cell lines. On the other hand, V3 and V4 did not show any cytotoxic effects across all tested cell lines. In contrast, V5 was toxic to HepG2 and MCF-7 cells but had no cytotoxic effect on Jurkat cell lines. V2 exhibited dose-dependent cytotoxicity (0-1000 <i>μ</i>g/mL), with the strongest inhibition observed against Jurkat cells (IC50 value = 96.341 <i>μ</i>g/mL) and a selective index of 3.567. The difference in activity between the extracts from different parts of the plant and the extract combined with lime juice was significant (<i>p</i> < 0.05), indicating a synergistic effect of the phytochemicals in both VAD and lime juice. <b>Conclusion:</b> V2 and V5 demonstrated a remarkable antioxidant property, and they are effective in inhibiting cancer cell lines, respectively.</p>","PeriodicalId":9007,"journal":{"name":"BioMed Research International","volume":"2024 ","pages":"9692656"},"PeriodicalIF":2.6,"publicationDate":"2024-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11540877/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142602933","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}