Journal of stem cell research & therapy最新文献

{"title":"Autologous Stromal Vascular Fraction Containing Stem Cells Combined with Low Intensity Shock Wave for the Treatment of Human Erectile Dysfunction","authors":"Elliot B. Lander, M. Berman","doi":"10.4172/2157-7633.1000438","DOIUrl":"https://doi.org/10.4172/2157-7633.1000438","url":null,"abstract":"Objective: This study seeks to determine whether the addition of autologous Adipose-Derived Stem Cells (ADSCs) may safely synergize with the clinical effects of Low-Intensity Shock Wave Therapy (LIST) to improve Erectile Dysfunction (ED) in a small series of men. Methods: Under IRB protocols, 52 patients with multifactorial ED were treated with LIST immediately followed by a single intracavernosal injection of lipoaspirate derived autologous Stromal Vascular Fraction (SVF). Human SVF derived using our protocols has been shown to contain Mesenchymal Stem Cells (MSCs) and Hematopoietic Stem Cells (HSCs). Subjective outcomes testing with the International Index of Erectile Function (IIEF) Score and Erectile Hardness Score (EHS) were administered to the patients at baseline and every 3 months for 2 years. All patients were queried for adverse reactions and all outcomes were self-reported. Results: Excellent safety was seen and none of the patients experienced any adverse reactions related to LIST or harvesting and deployment of SVF. A retrospective review of efficacy indicated that 37 out of 52 patients (71%) responded positively to the question of whether they experienced “overall improvement” after their treatment. Mean IIEF reference questionnaire scores and Mean Erectile Hardness Grading Score (EHS) were also reported by patients. Mean IIEF scores went from 10.21 baseline to 18.40 at 6 months (p=0.0008). Mean EHS scores improved from 1.34 baseline to 2.17 at 6 months (p=0.012). Conclusion: Intracavernosal deployment of autologous SVF combined with low-intensity acoustic shock wave therapy to the penis appears to be safe and may offer some benefits to a cross-section of patients who suffer from ED. Larger trials are indicated to identify which types of stem cells are optimal, which subsets of ED patients appear to benefit the most, and which types of shock wave protocols yield the best results. Autologous Stromal Vascular Fraction Containing Stem Cells Combined with Low Intensity Shock Wave for the Treatment of Human Erectile Dysfunction Elliot B Lander* and Mark H Berman Cell Surgical Network, Rancho Mirage, California, USA *Corresponding author: Elliot B Lander, Cell Surgical Network, Country Club Drive, Rancho Mirage, California, USA, Tel: 760-346-0145; Fax: 760-776-0041; E-mail: elliot@cellsurgicalnetwork.com Received August 03, 2018; Accepted September 11, 2018; Published September 17, 2018 Citation: Lander EB, Berman MH (2018) Autologous Stromal Vascular Fraction Containing Stem Cells Combined with Low Intensity Shock Wave for the Treatment of Human Erectile Dysfunction. Stem Cell Res Ther 8: 438. doi: 10.4172/21577633.1000438 Copyright: © 2018 Lander EB, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.","PeriodicalId":89694,"journal":{"name":"Journal of stem cell research & therapy","volume":"08 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70403453","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Anti-Inflammatory Therapeutic Development and Optimization of Umbilical Cord Tissue Derived Mesenchymal Stem Cells","authors":"M. Sutton, Sukhmani Kaur, K. Brown, M. L. Skiles, Michael Folz, A. Caplan, T. Bonfield","doi":"10.4172/2157-7633.1000435","DOIUrl":"https://doi.org/10.4172/2157-7633.1000435","url":null,"abstract":"Inflammation is important in the etio-pathogenesis of pulmonary diseases such as Cystic Fibrosis (CF), bronchoalveolar dysplasia and asthma, often contributing to severe morbidity and mortality. Human Mesenchymal Stem Cells (hMSCs) are known to have anti-inflammatory potential. Umbilical cord tissue is rich in hMSCs, thus making it a feasible option for anti-inflammatory hMSC therapeutic sourcing. Human Cord Tissue (HCT) derived hMSCs were evaluated for their functional potential to treat inflammation, with the hypothesis that HCT hMSCs secrete products that are anti-inflammatory, and that they alter epithelial cell expression of inflammatory properties as well. Further, these studies begin to identify optimized growth conditions to produce the most potent anti-inflammatory effect from the cord tissue derived hMSCs as well as address donor variability in hMSC sourcing. HCT hMSCs cultured with or without Lipopolysaccharide (LPS) or Peptidoglycan (PEP) were analyzed for secreted cytokines using Luminex and for gene expression utilizing RT-PCR and functionality by anti-inflammatory activity on epithelial cell line A549 cells. hMSCs expressed chemokines Interleukin 6 (IL-6), Interleukin 8 (IL-8), Chemokine Ligand 20 (CCL-20), Stem Cell Factor (SCF), and Monocyte Chemoattractant Protein-1 (MCP-1); LPS and PEP enhanced cytokine secretion. hMSCs secreted IL-6, IL-8, Macrophage Inflammatory Protein1-alpha (MIP1a), Tumor Necrosis Factor-alpha (TNF-a) and Interleukin 1-beta (IL-1B); with LPS and PEP stimulation following similar trends. HCT hMSCs showed IL-6 and IL-8 increased expression in functionality assay, which could be altered for optimization through changes in media glucose concentration. hMSCs secrete a variety of cytokines and display anti-inflammatory activity, with growth medium and donor variability. Optimization of HCT through growth medium as well as donor sourcing may be important for the development of disease specific therapeutics, but ultimately holds promise as a rich source for hMSCs.","PeriodicalId":89694,"journal":{"name":"Journal of stem cell research & therapy","volume":"08 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.4172/2157-7633.1000435","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70403386","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Review of the Placenta and Trophoblast Induced Pluripotent Stem Cells in Autism Spectrum Disorder Research","authors":"S. Jasani, G. Tartaglia, P. Yeung, Chiwei Lu","doi":"10.4172/2157-7633.1000413","DOIUrl":"https://doi.org/10.4172/2157-7633.1000413","url":null,"abstract":"Autism spectrum disorder (ASD) imparts a tremendous health burden with psychological, social, and economic implications. The biology of ASD is complex involving genetic, molecular, hormonal and immunologic factors however the convergence point of these various factors has not been identified as of yet. Limited evidence exists to suggest that the placenta may play such a governing role in ASD manifestation. The placenta is a neuroendocrine modulator by participating in the fetal hypothalamic pituitary gonadal (HPG) axis and also regulates the intrauterine environment mitigating fetal exposure to damaging factors to modulate the fetal stress response. Placental dysfunction has been associated with developmental abnormality and neuropsychiatric pathology adding to the biologic plausibility of the governing role the placenta may play in ASD development. By using current technology like induced pluripotent stem cells (iPSCs), a practical model system can be created to study ASD providing an alternative method to further research the placenta in ASD development","PeriodicalId":89694,"journal":{"name":"Journal of stem cell research & therapy","volume":"8 1","pages":"1-9"},"PeriodicalIF":0.0,"publicationDate":"2018-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.4172/2157-7633.1000413","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70402734","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Polytherapy with Reserpine and Glucagon-like Peptide-1 (GLP-1) Improves the Symptoms in Streptozotocin-Induced Type-1 Diabetic Mice by Reducing Inflammation and Inducting Beta Cell Regeneration","authors":"E. Skurikhin, O. Pershina, N. N. Ermakova, L. A. Ermolaeva, V. Krupin, A. Pakhomova, E. Pan, Marie-Theres Zeuner, D. Widera, E. S. Khmelevskaya, V. Fisenko, A. Dygai","doi":"10.4172/2157-7633.1000434","DOIUrl":"https://doi.org/10.4172/2157-7633.1000434","url":null,"abstract":"The present study investigated the effects of a polytherapy with Glucagon-like Peptide-1 (GLP-1) and the Vesicular Monoamine Transporter (VMAT) inhibitor reserpine on Streptozotocin (STZ)-induced type 1 diabetic mice. Methods: Experimental diabetes was induced in C57BL/6 mice by STZ. Reserpine and GLP-1 were applied intraperitoneally as polyor monotherapy. Results and Conclusion: We show for the first time that reserpine/GLP-1 polytherapy has a hypoglycaemic effect and increases the concentrations of insulin and GLP-1, reduces the level of IL-1β in the serum and infiltration of inflammatory cells into the pancreas, led to an increased number of multipotent beta cell progenitors, oligopotent beta cell precursors and Pdx1+ beta cells. We observed increased regeneration of beta cells in the diabetic pancreas. Hence, our approach could pave the way for developing new therapy approaches for Type 1 Diabetes (T1D). Polytherapy with Reserpine and Glucagon-like Peptide-1 (GLP-1) Improves the Symptoms in Streptozotocin-Induced Type-1 Diabetic Mice by Reducing Inflammation and Inducting Beta Cell Regeneration Evgenii Germanovich Skurikhin1, Olga Victorovna Pershina1*, Natalia Nikolaevna Ermakova1, Lubov Alexandrovna Ermolaeva1, Vyacheslav Andreevich Krupin1, Angelina Vladimirovna Pakhomova1, Edgar Sergeevich Pan1, Marie-Theres Zeuner2, Darius Widera2, Ekaterina Sergeevna Khmelevskaya1, Vladimir Petrovich Fisenko3 and Alexander Mikhaylovich Dygai1 1Laboratory of Regenerative Pharmacology, Goldberg ED Research Institute of Pharmacology and Regenerative Medicine, Tomsk National Research Medical Centre of the Russian Academy of Sciences, Tomsk, Russia 2Stem Cell Biology and Regenerative Medicine Group, Department of Pharmacology, School of Pharmacy, University of Reading, Reading, United Kingdom 3Department of Pharmacology, Sechenov First State Medical University, Russia *Corresponding author: Olga Victorovna Pershina, Laboratory of Regenerative Pharmacology, Goldberg ED Research Institute of Pharmacology and Regenerative Medicine, Tomsk National Research Medical Centre of the Russian Academy of Sciences, Tomsk, Russia, Tel: 8(3822) 3822-418-374; Fax: +7-3822-418-375; E-mail: ovpershina@gmail.com Received July 20, 2018; Accepted August 14, 2018; Published August 20, 2018 Citation: Skurikhin EG, Pershina OV, Ermakova NN, Ermolaeva LA, Krupin VA, et al. (2018) Polytherapy with Reserpine and Glucagon-like Peptide-1 (GLP1) Improves the Symptoms in Streptozotocin-Induced Type-1 Diabetic Mice by Reducing Inflammation and Inducting Beta Cell Regeneration. Stem Cell Res Ther 8: 434. doi: 10.4172/2157-7633.1000434 Copyright: © 2018 Skurikhin EG, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.","PeriodicalId":89694,"journal":{"name":"Journal of stem cell research & therapy","volume":"08 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70403303","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Reduction of Gingival Black Triangles with Dental Pulp or Gingival Stem Cell Injection Therapy: Clinical and Outcomes Research Gaps","authors":"Jennifer Díaz","doi":"10.23937/2469-570X/1410048","DOIUrl":"https://doi.org/10.23937/2469-570X/1410048","url":null,"abstract":"Gingival Black Triangles (GBTs), or gingival embrasures, are aesthetically displeasing even when they are not severe enough to require periodontal treatment. Multiple treatment modalities exist, including veneers, composite and other prosthetic modalities, with few, if any, treatments covered by insurance. Demand for GBT treatment is likely to increase with the aging of Baby Boomers, whose use of cosmetic dental procedures exceeds their predecessors. In recent days, stem cell therapy has gained more attention in the regeneration of oral and maxillofacial structures. We propose that the use of Dental Pulp Stem Cells (DPSC) or gingival stem cells for GBT treatment may have clinical, economic, and patient preference advantages that can fill a treatment gap, literally and figuratively. Safety and effectiveness concerns will persist until additional research is funded and conducted but the local nature of treatment would seem to expose patients to fewer risks than systemic use. Targeting the GBTs directly minimizes the long-term self-cleansing problem that other alternatives can create. Longevity is anticipated to exceed veneers and other prosthetic solutions. The cost of stem cell treatment for GBTs will likely be lower than veneers, consistent with the targeted placement. Further, less time in treatment and quicker recovery, both of which are anticipated, are likely to be preferable to patients. We enumerate these potential advantages and suggest a clinical way forward to evaluate the use of stem cell therapy for GBT treatment. Based on the anticipated differences for stem cell treatment of GBTs compared with usual care, we propose approaches for quantifying benefits from the patient perspective and from an economic standpoint. Future research will be needed to confirm the appropriateness of the recommended humanistic and economic evaluations.","PeriodicalId":89694,"journal":{"name":"Journal of stem cell research & therapy","volume":"4 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2017-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41598973","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Autologous Porcine Bone Marrow Mesenchymal Cells for Reconstruction of a Resorbed Alveolar Bone: A Preclinical Model in Mini-Pigs","authors":"Cecilie G Gjerde, D. Santis, M. Dominici, Zanotti Guglielmo, Hellem Sølve, Maria Serena Piccinno, J. Burns, A. Murgia, O. Candini, Krampera Mauro, Nocini Pierfrancesco, Addis Alessandro, A. Jerome, Layrolle Pierre, M. Kamal, Elena Veronesi","doi":"10.23937/2469-570X/1410050","DOIUrl":"https://doi.org/10.23937/2469-570X/1410050","url":null,"abstract":"Regeneration of atrophied alveolar bone prior to insertion of dental implants is a major challenge for oral and maxillofacial surgery. It has been reported that Bone Marrow (BM) derived Mesenchymal Stromal Cells (MSC) retain therapeutic potential for bone regeneration. In the present study, a preclinical mini-pig model simulating the clinical setting was established in order to evaluate the efficacy of autologous MSC for mandible regeneration. Under general anaesthesia, BM aspirates were collected from tibia of mini-pigs (n = 5) and MSC were isolated, characterized and expanded. At the same time, a narrow alveolar ridge was simultaneously created by bilateral extraction of two premolar teeth and removal of the buccal bone in order to simulate the pathological situation in humans. After ex vivo expansion, cells were delivered fresh to the surgical operating room and seeded on Biphasic Calcium Phosphate (BCP) granules for 1 hour followed by implantation into the simulated alveolar defects in one pig. The surgical defects were closed with sutures and left to heal for eight weeks. A bone biopsy was taken and dental implants were placed in the newly formed bone. The bone biopsy taken during the procedure showed mineralized bone containing substantial amount of new bone with BCP granules embedded in osteoid tissues and dispersed throughout the newly formed bone matrix. The data demonstrate the osteogenic potential of autologous MSC combined with BCP, providing crucial pre-clinical information in a large animal aimed at the reconstruction of resorbed alveolar bone.","PeriodicalId":89694,"journal":{"name":"Journal of stem cell research & therapy","volume":"4 1","pages":"1-11"},"PeriodicalIF":0.0,"publicationDate":"2017-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42908045","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Adult Stem Cell Therapy in Liver Cirrhosis Management: Current Practices and Future Perspectives","authors":"Gounder Sellamuthu Subbanna, K. Radha, C. S. Choy, Veerakumarasivam Abhi, S. Baskar","doi":"10.23937/2469-570X/1410049","DOIUrl":"https://doi.org/10.23937/2469-570X/1410049","url":null,"abstract":"","PeriodicalId":89694,"journal":{"name":"Journal of stem cell research & therapy","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2017-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45079766","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Advanced biomaterial PepGel - a new tool for translational research in cell therapy","authors":"X. Sun","doi":"10.4172/2157-7633-C1-028","DOIUrl":"https://doi.org/10.4172/2157-7633-C1-028","url":null,"abstract":"Cancer ruins one of the utmost lethal ailments for human. Oncogenic fusion gene is one of the significant mechanisms driving the evolution of human cancers. MAN2A1-FER, a fusion gene among the mannosidase domain of MAN2A1 and tyrosine kinase domain of FER, was found in 6 diverse sorts of human malignancies. MAN2A1-FER fusion translocated FER domain from cytoplasm to golgi apparatus, and directed to phosphorylation of N-terminus of EGFR and establishment of EGFR signaling path. Expression of MAN2A1-FER produced vivid increase of development and incursion of cancers, while elimination of MAN2A1-FER through knockout created important lower level of growth and metastasis. The presence of MAN2A1-FER improved the compassion of human cancers to FER kinase inhibitor crizotinib or EGFR kinase inhibitor canertinib. Hydrodynamic tail-vein injection of MAN2A1-FER gene caused in quick expansion of liver cancer in pests with somatic Pten deletion. Taken together, we determined that MAN2A1-FER fusion gene is one of the vital drivers for human cancer development.","PeriodicalId":89694,"journal":{"name":"Journal of stem cell research & therapy","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2017-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70410010","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cell-engineered construct for the regeneration of damaged articular cartilage","authors":"V. Sevastianov","doi":"10.4172/2157-7633-C1-030","DOIUrl":"https://doi.org/10.4172/2157-7633-C1-030","url":null,"abstract":"","PeriodicalId":89694,"journal":{"name":"Journal of stem cell research & therapy","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2017-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70410101","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"RhoC-Rock2 signaling regulate radio-resistance in cervical carcinoma by modulating DNA repair activity","authors":"C. Ross","doi":"10.4172/2157-7633-C1-029","DOIUrl":"https://doi.org/10.4172/2157-7633-C1-029","url":null,"abstract":"","PeriodicalId":89694,"journal":{"name":"Journal of stem cell research & therapy","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2017-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70410082","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}