Journal of stem cell research & therapy最新文献_第8页

CCR5 governs stem cell characteristics, therapy resistance and metastasis of breast cancer CCR5控制癌症干细胞特征、治疗耐药性和转移

Journal of stem cell research & therapy Pub Date : 2017-10-28 DOI: 10.4172/2157-7633-C1-025

R. Pestell

引用次数: 0

c-kit Positive Cardiac Outgrowth Cells Demonstrate Better Ability for Cardiac Recovery Against Ischemic Myopathy. c-kit阳性心脏外生细胞对缺血性肌病有更好的心脏恢复能力。

Journal of stem cell research & therapy Pub Date : 2017-10-01 Epub Date: 2017-10-13 DOI: 10.4172/2157-7633.1000402

Chuan Li, Satoshi Matsushita, Zhengqing Li, Jianjun Guan, Atsushi Amano

{"title":"c-kit Positive Cardiac Outgrowth Cells Demonstrate Better Ability for Cardiac Recovery Against Ischemic Myopathy.","authors":"Chuan Li, Satoshi Matsushita, Zhengqing Li, Jianjun Guan, Atsushi Amano","doi":"10.4172/2157-7633.1000402","DOIUrl":"https://doi.org/10.4172/2157-7633.1000402","url":null,"abstract":"Objective: Resident cardiac stem cells are expected to be a therapeutic option for patients who suffer from severe heart failure. However, uncertainty remains over whether sorting cells for c-kit, a stem cell marker, improves therapeutic outcomes.Materials and methods: Cardiac outgrowth cells cultured from explants of rat heart atrium were sorted according to their positivity (+) or negativity (-) for c-kit. These cells were exposed to hypoxia for 3 d, and subsequently harvested for mRNA expression measurement. The cell medium was also collected to assess cytokine secretion. To test for a functional benefit in animals, myocardial infarction (MI) was induced in rats, and c-kit+ or c-kit- cells were injected. The left ventricular ejection fraction (LVEF) was measured for up to 4 weeks, after which the heart was harvested for biological and histological analyses.Results and conclusion: Expression of the angiogenesis-related genes, VEGF and ANGPTL2, was significantly higher in c-kit+ cells after 3 d of hypoxic culture, although we found no such difference prior to hypoxia. Secretion of VEGF and ANGPTL2 was greater in the c-kit+ group than in the c-kit- group, while hypoxia tended to increase cytokine expression in both groups. In addition, IGF-1 was significantly increased in the c-kit+ group, consistent with the relatively low expression of cleaved-caspase 3 revealed by western blot assay, and the relatively low count of apoptotic cells revealed by histochemical analysis. Administration of c-kit+cells into the MI heart improved the LVEF and increased neovascularization. These results indicate that c-kit+cells may be useful in cardiac stem cell therapy.","PeriodicalId":89694,"journal":{"name":"Journal of stem cell research & therapy","volume":"7 10","pages":""},"PeriodicalIF":0.0,"publicationDate":"2017-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.4172/2157-7633.1000402","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35654688","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 9

Second Generation TKI before and after Stem Cell Transplant for CML Blast Crisis in the Era of Precision Medicine, Where Do We Go from There? 精准医学时代干细胞移植治疗慢性粒细胞白血病爆发危机前后的第二代TKI，我们将何去何从？

Journal of stem cell research & therapy Pub Date : 2017-09-25 DOI: 10.4172/2157-7633.1000401

S. Farhan

引用次数: 0

Therapeutic Potential of Selenium Treated Stem Cells for the Reduction of Liver Fibrosis 硒处理的干细胞对减少肝纤维化的治疗潜力

Journal of stem cell research & therapy Pub Date : 2017-09-12 DOI: 10.4172/2157-7633.1000399

S. Shams, M. Ayaz, S. G. Afridi, H. Khan

{"title":"Therapeutic Potential of Selenium Treated Stem Cells for the Reduction of Liver Fibrosis","authors":"S. Shams, M. Ayaz, S. G. Afridi, H. Khan","doi":"10.4172/2157-7633.1000399","DOIUrl":"https://doi.org/10.4172/2157-7633.1000399","url":null,"abstract":"Mesenchymal Stem Cells (MSCs) therapy is an alternative way to treat liver fibrosis. The aim of the current study is to enhance the therapeutic potential of MSCs by pretreated with selenium for the reduction of CCl4 induced liver injury. Male Balb/C mice were treated with CCl4 (1.0 μL/g) intraperitoneally, twice a week for 4 weeks. Mouse MSCs were cultured and then pretreated with 15 ng/ml selenium for 24 hrs. The untreated and selenium pretreated MSCs were transplanted into CCl4 injured mice. After two weeks of MSCs transplantation, mice were observed for liver regeneration. The morphological result showed that selenium treated MSCs have significant therapeutic effect in reduction of CCl4 induced injured as compared to untreated MSCs. Biochemical and histopathological result also revealed significant reduction in serum ALT and bilirubin level, collagen content in selenium treated MSCs group as compared to untreated MSCs. Reverse transcriptase PCR result at mRNA level also confirm the antifibrotic effect of selenium treated MSCs on liver fibrosis as evidenced by decreasing the expression level of apoptotic marker and enhancing hepatocyte marker. Thus it is concluded that selenium treated MSCs have a strong therapeutic effect on the reduction of liver fibrosis in CCl4 mice model.","PeriodicalId":89694,"journal":{"name":"Journal of stem cell research & therapy","volume":" ","pages":"1-6"},"PeriodicalIF":0.0,"publicationDate":"2017-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.4172/2157-7633.1000399","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44884701","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Role of Adipose-Derived Mesenchymal Stem Cells in Full Thickness Wound Healing 脂肪源性间充质干细胞在全层伤口愈合中的作用

Journal of stem cell research & therapy Pub Date : 2017-09-05 DOI: 10.4172/2157-7633.1000398

I. Dososaputro, L. Hoekstra, Dinar Rahmania, D. S. Perdanakusuma

{"title":"Role of Adipose-Derived Mesenchymal Stem Cells in Full Thickness Wound Healing","authors":"I. Dososaputro, L. Hoekstra, Dinar Rahmania, D. S. Perdanakusuma","doi":"10.4172/2157-7633.1000398","DOIUrl":"https://doi.org/10.4172/2157-7633.1000398","url":null,"abstract":"Background: Wound healing problems can arise in donor wounds after harvesting a full-thickness graft. Returning Mesenchymal Stem Cells (MSCs) on the wounds may accelerate wound healing. The aim of this study is to analyse the effect of MSCs in the epithelialization process and collagen density on full-thickness wound healing. \u0000Methods: The pilot study included 10 patients undergoing excision of a full-thickness skin graft on the groin. Patients were randomly divided into two groups: Mesenchymal Stem Cells (MSCs) and Non-Mesenchymal Stem Cells (Non-MSCs). The MSCs group had previously undergone fat harvesting which was processed into mesenchymal stem cells. Biopsies were taken from both groups on days 14 (proliferative phase) and 45 (maturation phase), and were compared with normal skin (NS; n=5). Epithelial layers of the epidermis were assessed with hematoxylin eosin staining. Collagen density was evaluated with MT staining, and analysed using a light microscope. \u0000Result: In the MSCs group and the Non-MSCs group, the number of epithelial layers were significantly higher compared to the NS-group on day 45 (14.7 ± 0.70 and 8.24 ± 0.76 vs 5.43 ± 0.60 respectively; p<0.001 and p<0.001). The collagen density in the MSCs group on day 14 was 33.3 ± 2.46% in the MSCs group and 45.7 ± 5.84% in the non-MSCs group, compared to 54.3 ± 3.71% in the NS-group (p<0.001 and p=0.012 resp.). These values increased on day 45 to 49.2 ± 3.28% in the MSCs group, and 73.4 ± 1.63% in the non-MSCs group. \u0000Conclusion: Mesenchymal stem cells increased the number of epithelial layers in the full-thickness wound healing process compared to normal skin. A higher increase was seen in the MSCs-group. On day 45, an increase in collagen density was observed in the MSCs group and Non-MSCs group. Adipose-derived mesenchymal stem cells can be used in the process of full-thickness wound healing. Future randomized controlled trials are needed to confirm these findings.","PeriodicalId":89694,"journal":{"name":"Journal of stem cell research & therapy","volume":"7 1","pages":"1-6"},"PeriodicalIF":0.0,"publicationDate":"2017-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.4172/2157-7633.1000398","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43829299","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Morphological Evidence of Telocytes in Canine Inferior Vena Cava 犬下腔静脉Tel细胞的形态学证据

Journal of stem cell research & therapy Pub Date : 2017-08-21 DOI: 10.4172/2157-7633.1000397

Ting Xu, Xinyu Lu, Shanshan Lu, Jing Cheng, X. Zhou, Hongqi Zhang

引用次数: 1

Acute Genotoxic Stress-Induced Senescence in Human Mesenchymal Cells Drives a Unique Composition of Senescence Messaging Secretome (SMS) 急性基因毒性应激诱导的人间充质细胞衰老驱动衰老信息分泌组（SMS）的独特组成

Journal of stem cell research & therapy Pub Date : 2017-08-20 DOI: 10.4172/2157-7633.1000396

M. Gaur, Lu Wang, Alex, ra Amaro-Ortiz, M. Dobke, I. Jordan, V. Lunyak

{"title":"Acute Genotoxic Stress-Induced Senescence in Human Mesenchymal Cells Drives a Unique Composition of Senescence Messaging Secretome (SMS)","authors":"M. Gaur, Lu Wang, Alex, ra Amaro-Ortiz, M. Dobke, I. Jordan, V. Lunyak","doi":"10.4172/2157-7633.1000396","DOIUrl":"https://doi.org/10.4172/2157-7633.1000396","url":null,"abstract":"MSC mediate numerous therapeutic effects by promoting repair directly via differentiation into critical cell types or indirectly through the secretion of a broad spectrum of soluble factors with diverse paracrine activities. However, recent discoveries indicate that MSC are sensitive to endogenous and exogenous stressors and many diseaserelated interventions can result in Therapy-Induced Senescence (TIS) in MSC. Here, we provide a detailed analysis of changes in secretory factors that occur under conditions of bleomycin treatment that triggers genotoxic stressinduced senescence of Human Adipose-Derived Stem Cells (hADSCs). The bleomycin treatment considerably alters the composition of hADSCs secretome. Our data reveal the novel unique composition of Senescence Messaging Secretome (SMS) of hADSCs and suggest that this SMS might critically influence genotoxic drug-based or MSC combinational therapies by imposing interference with tissue homeostasis, metabolism and regeneration in autocrine and paracrine fashion. SMS can compromise MSC-mediated immunological responses and their regenerative potential. Our findings underscore the importance of careful evaluation of stress-induced senescence of adult stem cells in regenerative and combination therapies.","PeriodicalId":89694,"journal":{"name":"Journal of stem cell research & therapy","volume":" ","pages":"1-13"},"PeriodicalIF":0.0,"publicationDate":"2017-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48051133","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

Dopaminergic enhancement of cellular adhesion in bone marrow derived mesenchymal stem cells (MSCs). 多巴胺能增强骨髓间充质干细胞(MSCs)的细胞粘附。

Journal of stem cell research & therapy Pub Date : 2017-08-01 Epub Date: 2017-08-10 DOI: 10.4172/2157-7633.1000395

Si Chen, Bing Bai, Dong Joon Lee, Shannon Diachina, Yina Li, Sing Wai Wong, Zhengyan Wang, Henry C Tseng, Ching-Chang Ko

{"title":"Dopaminergic enhancement of cellular adhesion in bone marrow derived mesenchymal stem cells (MSCs).","authors":"Si Chen, Bing Bai, Dong Joon Lee, Shannon Diachina, Yina Li, Sing Wai Wong, Zhengyan Wang, Henry C Tseng, Ching-Chang Ko","doi":"10.4172/2157-7633.1000395","DOIUrl":"https://doi.org/10.4172/2157-7633.1000395","url":null,"abstract":"Dopamine (DA) is a well-known neurotransmitter and critical element in the mussel adhesive protein that has gained increasing attention for its role in cellular growth enhancement in biomaterials, including cellular adhesion improvement. As the mechanism underlying this remains unclear, the objective of this study was to explore the effects of DA on the adhesion properties of bone marrow derived rat mesenchymal stem cells (rMSCs) using an hydroxyapatite gelatin nanocomposite biomaterial and to test whether the effects are mediated through various endogenously expressed DA receptors. Primary rMSCs were pretreated with D1-like antagonist, D2-like antagonist, or a combination of these antagonists followed by treatment with 50 μM DA and cellular adhesion quantification at 0.5, 1, 2 and 4 hours post DA addition. DA was found to increase rMSC adhesion and spreading at the 0.5 hour time-point and the dopaminergic effect on cell adhesion was partially blocked by DA antagonists. In addition, the D1-like and D2-like antagonists appeared to have a similar effect on rMSCs. Immunofluorescent staining indicated that the rMSC spreading area was significantly increased in the DA treated group versus the control group. Treatment of the D1-like DA antagonists with DA revealed that the actin filaments of rMSCs could not connect the membrane with the nucleus. In summary, DA was found to enhance early rMSC adhesion partially via DA receptor activation.","PeriodicalId":89694,"journal":{"name":"Journal of stem cell research & therapy","volume":"7 8","pages":""},"PeriodicalIF":0.0,"publicationDate":"2017-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.4172/2157-7633.1000395","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35754036","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 9

Mesenchymal Stem Cell Soluble Mediators and Cystic Fibrosis. 间充质干细胞可溶性介质与囊性纤维化。

Journal of stem cell research & therapy Pub Date : 2017-08-01 Epub Date: 2017-09-22 DOI: 10.4172/2157-7633.1000400

Morgan T Sutton, David Fletcher, Nicole Episalla, Lauren Auster, Sukhmani Kaur, Mary Chandler Gwin, Michael Folz, Dante Velasquez, Varun Roy, Rolf van Heeckeren, Donald P Lennon, Arnold I Caplan, Tracey L Bonfield

{"title":"Mesenchymal Stem Cell Soluble Mediators and Cystic Fibrosis.","authors":"Morgan T Sutton, David Fletcher, Nicole Episalla, Lauren Auster, Sukhmani Kaur, Mary Chandler Gwin, Michael Folz, Dante Velasquez, Varun Roy, Rolf van Heeckeren, Donald P Lennon, Arnold I Caplan, Tracey L Bonfield","doi":"10.4172/2157-7633.1000400","DOIUrl":"https://doi.org/10.4172/2157-7633.1000400","url":null,"abstract":"Human Mesenchymal stem cells (hMSCs) secrete products (supernatants) that are anti-inflammatory and antimicrobial. We have previously shown that hMSCs decrease inflammation and Pseudomonas aeruginosa infection in the in vivo murine model of Cystic Fibrosis (CF). Cystic Fibrosis (CF) is a genetic disease in which pulmonary infection and inflammation becomes the major cause of morbidity and mortality. Our studies focus on determining how MSCs contribute to improved outcomes in the CF mouse model centering on how the MSCs impact the inflammatory response to pathogenic organisms. We hypothesize that MSCs secrete products that are anti-inflammatory in scenarios of chronic pulmonary infections using the murine model of infection and inflammation with a specific interest in Pseudomonas aeruginosa (gram negative). Further, our studies will identify whether the MSCs are impacting this inflammatory response through the regulation of peroxisome proliferator activator receptor gamma (PPARγ) which aides in decreasing inflammation.","PeriodicalId":89694,"journal":{"name":"Journal of stem cell research & therapy","volume":"7 9","pages":""},"PeriodicalIF":0.0,"publicationDate":"2017-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.4172/2157-7633.1000400","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35700027","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 26

Second Generation Tyrosine Kinase Inhibitors Combined With Allogeneic Hematopoietic Stem Cell Transplantation Improve the Prognosis of Patients with Chronic Myelogenous Leukemia in Blast Crisis 第二代酪氨酸激酶抑制剂联合异基因造血干细胞移植改善慢性髓性白血病原细胞危重期患者的预后

Journal of stem cell research & therapy Pub Date : 2017-07-31 DOI: 10.4172/2157-7633.1000393

Zheng-ping Yu, Jiahua Ding, A. Sun, Z. Ge, Bao‐an Chen, W. He

{"title":"Second Generation Tyrosine Kinase Inhibitors Combined With Allogeneic Hematopoietic Stem Cell Transplantation Improve the Prognosis of Patients with Chronic Myelogenous Leukemia in Blast Crisis","authors":"Zheng-ping Yu, Jiahua Ding, A. Sun, Z. Ge, Bao‐an Chen, W. He","doi":"10.4172/2157-7633.1000393","DOIUrl":"https://doi.org/10.4172/2157-7633.1000393","url":null,"abstract":"The prognosis for patients with Chronic Myelogenous Leukemia (CML) in Blastic Crisis (BC) is poor, with a median survival of only 3-6 months. Blast crisis (BC) is highly refractory to therapy and has a poor prognosis. To determine the efficacy of TKIs-II combined with allogeneic hematopoietic stem cell transplantation (allo-HSCT) in CML BC, we present four consecutive, recent cases of CML BC in which TKIs-II were used before or after allo-HSCT. Patient 1, a 28-year-old male received a HSCT from a half-matched, related-donor. Patient 2, 3 received an HLAidentical unrelated-donor HSCT. To date, patients 1,2,3 and 4 have survived postprocedure for 22, 23, 21 and 25 months, respectively. We conclude that compared with imatinib, TKIs-II may reduce tumor burden more rapidly and thoroughly when administered before or after allo-HSCT and enhance the graft versus leukemia effect, prolonging the long-term survival of patients. We speculate that GVL and tumor burden are negatively correlated.","PeriodicalId":89694,"journal":{"name":"Journal of stem cell research & therapy","volume":" ","pages":"1-6"},"PeriodicalIF":0.0,"publicationDate":"2017-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.4172/2157-7633.1000393","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43832154","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1