Journal of stem cell research & therapy最新文献_第10页

Complex Treatment of Multiple Sclerosis Patients by Use of Fetal StemCells 胎儿干细胞用于多发性硬化症患者的复杂治疗

Journal of stem cell research & therapy Pub Date : 2017-05-08 DOI: 10.4172/2157-7633.1000386

N. Sych, M. Klunnyk, I. Matiyashchuk, M. Demchuk, O. Ivankova, Andriy Sinelnyk, M. Skalozub

{"title":"Complex Treatment of Multiple Sclerosis Patients by Use of Fetal StemCells","authors":"N. Sych, M. Klunnyk, I. Matiyashchuk, M. Demchuk, O. Ivankova, Andriy Sinelnyk, M. Skalozub","doi":"10.4172/2157-7633.1000386","DOIUrl":"https://doi.org/10.4172/2157-7633.1000386","url":null,"abstract":"Objective: Refinement for all existing methods of treatment for the multiple sclerosis patients by means of combined method including conventional therapy and administration of fetal stem cells (FSCs)-suspensions containing stem cells derived from human fetal liver and brain. \u0000Materials and methods: 51 patients diagnosed with MS participated in the study including 27 men and 24 women aged 27 to 56 years. Average age range for men was 34.2 ± 1.2 years, for women it constituted 31.7 ± 1.3 years. 33 patients were allocated in the Main Group (MG) including 20 men (mean age 29.8 ± 2.2 years) and 13 women (average age 31.3 ± 2.1 years). The Control Group (CG) was composed of 18 patients diagnosed with MSincluding 10 men (mean age of 30.5 ± 1.2 years) and 8 women (average age 31.4 ± 1.4 years). Simultaneously, the scale of MMSE was applied for objective study of cognitive functions among our patients. State Trait Anxiety Inventory was effective for emotional-anxiety disturbances. In addition, we assessed depression in patients by means of Beck Depression Inventory. \u0000Results: Authors proved efficacy and safety of FSCs suspensions for MS patients. We emphasized a significant improvement of neurology deficit in the MG already over 6 months after fetal stem cells transplantation (FSCT), whereas the CG patients revealed such advantages over 12 months. Improvement of cognitive functions was characteristic for the MG starting from 6 months after FSCT. Anxiety and depression significantly decreased and these results were observed over 6 months after FSCT in the MG. In the CG, the same values which significantly decreased over 12 months after treatment and were recorded. \u0000Conclusion: FSCs use in complex treatment of patients with MS stabilizes disease compensation, improves cognitive functions as well as psycho-emotional state of the patients.","PeriodicalId":89694,"journal":{"name":"Journal of stem cell research & therapy","volume":" ","pages":"1-7"},"PeriodicalIF":0.0,"publicationDate":"2017-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47955923","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

A Forecast of Targeting Leukemia Stem Cells by Nanomedicine 纳米医学靶向白血病干细胞研究进展展望

Journal of stem cell research & therapy Pub Date : 2017-05-08 DOI: 10.4172/2157-7633.1000385

Xiangwei Huang, X. Kang, Meng Zhao

引用次数: 2

Hyaluronic Acid Mediated Enrichment of CD44 Expressing Glioblastoma Stem Cells in U251MG Xenograft Mouse Model 透明质酸在U251MG异种移植小鼠模型中介导CD44表达胶质母细胞瘤干细胞的富集

Journal of stem cell research & therapy Pub Date : 2017-04-10 DOI: 10.4172/2157-7633.1000384

Arun Vaidyanath, Hafizah Mahmud, A. C. Khayrani, Aung Ko Ko Oo, Akimasa Seno, Mami Asakura, T. Kasai, M. Seno

{"title":"Hyaluronic Acid Mediated Enrichment of CD44 Expressing Glioblastoma Stem Cells in U251MG Xenograft Mouse Model","authors":"Arun Vaidyanath, Hafizah Mahmud, A. C. Khayrani, Aung Ko Ko Oo, Akimasa Seno, Mami Asakura, T. Kasai, M. Seno","doi":"10.4172/2157-7633.1000384","DOIUrl":"https://doi.org/10.4172/2157-7633.1000384","url":null,"abstract":"Background: Glioblastoma is one of the most aggressive cancer with high mortality rates and poses several hurdles in the efficient chemotherapeutic intervention. Similar to other cancers, glioma also harbors CSCs, that are self-renewable, multipotent cells, which initiate the cancer incidence, chemotherapeutic resistance and cancer recurrence. The microenvironmental regulation in the brain tumor and metastasis involves the cooperative interaction between HA and CD44. CD44, being a multifaceted transmembrane glycoprotein by itself, or in combination with several other cell surface receptors, has been used as a marker for CSC isolation. \u0000Methods: We established both adherent and nonadherent culture of U251MG cells by treating with high molecular weight HA. Further these cells were transplanted subcutaneously in Balb/c mouse for the generation of the xenograft model for the cancer stem cell. The tumor was further characterized for the establishment of the working model for molecular targeting studies of cancer stem cells. \u0000Results: Here we showed the enrichment of the CD44 expressing population of glioblastoma cells by induction with hyaluronic acid. The non-adherent culture spheroids of U251MG cells showed up regulation in the CD44 expression along with aberrant activation of principal pluripotency genes OCT3/4, SOX2, KLF4 and Nanog. Using the HA-treated spheroid, we established an experimental xenograft mouse model with high angiogenesis enhanced tumor-initiating capacity while retaining the glioblastoma traits. \u0000Conclusion: We characterized a mouse xenograft model of U251MG cells which could be a promising model system to study the molecular targeting approaches against CSCs in glioblastoma.","PeriodicalId":89694,"journal":{"name":"Journal of stem cell research & therapy","volume":"7 1","pages":"1-8"},"PeriodicalIF":0.0,"publicationDate":"2017-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42176718","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 14

Cellular therapy, an autologous cellular PoC approach to satisfy patients needs 细胞疗法，一种满足患者需要的自体细胞PoC方法

Journal of stem cell research & therapy Pub Date : 2017-04-04 DOI: 10.4172/2157-7633.C1.022

Falk Heinrichsohn

引用次数: 0

Cell therapy and its emerging markets 细胞疗法及其新兴市场

Journal of stem cell research & therapy Pub Date : 2017-04-04 DOI: 10.4172/2157-7633.C1.024

Deven Patel

引用次数: 1

Combined Jellyfish Collagen Type II, Human Stem Cells and Tgf-β3 as a Therapeutic Implant for Cartilage Repair 水母II型胶原、人干细胞和生长因子-β3联合作为软骨修复的治疗性植入物

Journal of stem cell research & therapy Pub Date : 2017-03-27 DOI: 10.4172/2157-7633.1000382

M. Pugliano, Xavier Vanbellinghen, P. Schwinté, N. Benkirane-Jessel, L. Keller

{"title":"Combined Jellyfish Collagen Type II, Human Stem Cells and Tgf-β3 as a Therapeutic Implant for Cartilage Repair","authors":"M. Pugliano, Xavier Vanbellinghen, P. Schwinté, N. Benkirane-Jessel, L. Keller","doi":"10.4172/2157-7633.1000382","DOIUrl":"https://doi.org/10.4172/2157-7633.1000382","url":null,"abstract":"Background: The limitations associated to current therapies for articular cartilage repair led us to develop new strategies of applicable active therapeutic materials. Human mesenchymal stem cells from bone marrow are promising relevant cell sources for cell therapy and regenerative medicine, in particular for cartilage repair. Recently, a new source of non-mammalian collagen type II emerged and represents a promising tool for cartilage tissue engineering. \u0000Methods: To develop a new therapeutic implant for cartilage repair, we combined (i) jellyfish collagen type II as an implant; (ii) active nanoreservoirs of growth factors (TGF-β3); (iii) adult human mesenchymal stem cells derived from bone marrow. \u0000Results: Our results indicated clearly that (i) the jellyfish collagen type II implant leads to chondrogenic differentiation of mesenchymal stem cells; (ii) the combined implant and active therapeutic TGF-β3 as nanoreservoirs lead to chondrogenic gene expression and cartilage differentiation. \u0000Conclusion: We reported here a new stem cell - based therapeutic active implant for cartilage repair. This approach combines jellyfish collagen type II, human stem cells and TGF-β3 as a therapeutic implant to improve cartilage differentiation and repair.","PeriodicalId":89694,"journal":{"name":"Journal of stem cell research & therapy","volume":"7 1","pages":"1-9"},"PeriodicalIF":0.0,"publicationDate":"2017-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.4172/2157-7633.1000382","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45954537","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 25

Current Stage and Future Perspective of Stem Cell Therapy in IschemicStroke 缺血性脑卒中干细胞治疗的现状与展望

Journal of stem cell research & therapy Pub Date : 2017-03-23 DOI: 10.4172/2157-7633.1000381

Buga Ana-Maria, Albu Carmen, Tudorica Valerica

引用次数: 3

Complex Treatment of Erectile Dysfunction with Vascular Factor Deficitby Use of Pluripotent Fetal Stem Cells 应用多能干细胞综合治疗血管因子缺陷型勃起功能障碍

Journal of stem cell research & therapy Pub Date : 2017-03-21 DOI: 10.4172/2157-7633.1000380

M. Klunnyk, I. Matiyashchuk, N. Sych, Andriy Sinelnyk, Miroshnykov Io, K. Sorochynska

{"title":"Complex Treatment of Erectile Dysfunction with Vascular Factor Deficitby Use of Pluripotent Fetal Stem Cells","authors":"M. Klunnyk, I. Matiyashchuk, N. Sych, Andriy Sinelnyk, Miroshnykov Io, K. Sorochynska","doi":"10.4172/2157-7633.1000380","DOIUrl":"https://doi.org/10.4172/2157-7633.1000380","url":null,"abstract":"Objective: To study the effect of combined therapy including fetal stem cells (FSCs) on different components of erections. \u0000Material and methods: During a period of 2014-2015 years 19 patients with mixed type erectile dysfunction and mandatory vascular factor documented by pharmacodopplerography were under study in Cell Therapy Center EmCell. The program of examination for patients in the Main Group (MG) and Control Group (CG) included the scales of International Index of Erectile Function (IIEF) questionnaire, urine analysis, tests for total and free testosterone, Sex Hormone Binding Globulin (SHBG), Free Androgen Index (FAI), blood glucose and glycosylated Hemoglobin (HbA1c) testing, lipidogram findings, pharmacodopplerography of cavernous vessels, study of cortical and diencephalon components of erection by use of clinical methods and the questionnaires of State-Trait Anxiety Inventory – (STAI) and Beck Depression Inventory (BDI). \u0000Conclusion: combined use of vardenafil and cryopreserved suspensions containing pluripotent FSCs extracted from fetal liver, brain and placenta promotes favorable effect on all components of erections.","PeriodicalId":89694,"journal":{"name":"Journal of stem cell research & therapy","volume":" ","pages":"1-6"},"PeriodicalIF":0.0,"publicationDate":"2017-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45589695","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Graft Composition and Post-Thawing Cell Viability Influence the Hematopoietic Recovery in Autologous Hematopoietic Stem Cell Transplantation 移植物成分和解冻后细胞活力对自体造血干细胞移植中造血功能恢复的影响

Journal of stem cell research & therapy Pub Date : 2017-03-20 DOI: 10.4172/2157-7633.1000379

C. Valentini, M. Bianchi, Nicoletta Orl, F. Autore, M. G. Iachininoto, N. Piccirillo, S. Sica, G. Zini, V. Stefano, L. Teofili

{"title":"Graft Composition and Post-Thawing Cell Viability Influence the Hematopoietic Recovery in Autologous Hematopoietic Stem Cell Transplantation","authors":"C. Valentini, M. Bianchi, Nicoletta Orl, F. Autore, M. G. Iachininoto, N. Piccirillo, S. Sica, G. Zini, V. Stefano, L. Teofili","doi":"10.4172/2157-7633.1000379","DOIUrl":"https://doi.org/10.4172/2157-7633.1000379","url":null,"abstract":"Objective: We investigated the influence of graft composition and post-thawing cell viability on the hematopoietic \u0000 recovery of patients undergoing Autologous Hematopoietic Stem Cell Transplantation (ASCT). \u0000 Methods: Data relative to 146 ASCT procedures performed in 134 patients were examined. The doses of \u0000 CD34+ cells and parameters related to the grafts’ composition (white blood cell – WBC, neutrophil and platelet -PLT \u0000 concentrations) were correlated to the number of days for neutrophil and platelet engraftment. Moreover, patients \u0000 were grouped according to the values of post-thawing total nucleated cell (TNC) and CD34+ cell viability (as lower of \u0000 higher than median values of the entire series) and hematopoietic recovery was accordingly evaluated. \u0000 Results: The CD34+ cell dose significantly predicts both neutrophil and PLT engraftment. Patients with low TNC \u0000 viability had longer time to neutrophil engraftment, while patients with low CD34+ cell viability exhibited longer time \u0000 to both neutrophil and PLT engraftment. High WBC or neutrophil concentrations in the graft were associated with low \u0000 TNC viability. In addition, we found an inverse correlation between CD34+ cell viability and graft PLT concentration. Conclusions: Our findings suggest that the platelet content in apheresis products is a critical issue, with an impact \u0000 on CD34+ cell viability and hematopoietic recovery after ASCT. The apheresis products containing high amounts of \u0000 PLT should be evaluated for PLT removal before freezing.","PeriodicalId":89694,"journal":{"name":"Journal of stem cell research & therapy","volume":" ","pages":"1-4"},"PeriodicalIF":0.0,"publicationDate":"2017-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48765645","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Complex Treatment of the Patients with Cerebral Palsy Using Fetal Stem Cells 胎儿干细胞综合治疗脑瘫

Journal of stem cell research & therapy Pub Date : 2017-03-15 DOI: 10.4172/2157-7633.1000378

N. Sych, M. Klunnyk, I. Matiyashchuk, M. Demchuk, O. Ivankova, Andriy Sinelnyk, M. Skalozub, Khrystyna Sorochunska

{"title":"Complex Treatment of the Patients with Cerebral Palsy Using Fetal Stem Cells","authors":"N. Sych, M. Klunnyk, I. Matiyashchuk, M. Demchuk, O. Ivankova, Andriy Sinelnyk, M. Skalozub, Khrystyna Sorochunska","doi":"10.4172/2157-7633.1000378","DOIUrl":"https://doi.org/10.4172/2157-7633.1000378","url":null,"abstract":"Objective: The aim of this study was to examine fetal stem cells (FSCs) treatment safety and efficacy in complex therapy for cerebral palsy (CP) children. We studied clinical, neurological, and laboratory changes in CP patients before and after FSCs therapy. \u0000Materials and methods: 11 children diagnosed with CP were under observation in Cell Therapy Center EmCell including 6 boys aged from 2 to 13 years, whose mean age was 4.23 ± 0.24 yrs. and 5 girls aged from 2.5 to 12 years with mean age which constituted 3.92 ± 0.15 yrs. The patients were allocated to the Main Group (MG) who along with standard treatment received FSCs. Control group (CG) included 9 patients suffering from CP who underwent treatment solely with inclusion of conventional methods and consisted of 4 males (aged from 3 to 14 yrs. whose average age made up 4.01 ± 0.12 yrs.) and 5 females in age from 3 to 15 yrs. with mean age by 3.87 ± 0.18 yrs. FSCs were administered as a complex therapy for the patients with CP and the data of clinical and laboratory investigations were analyzed during the whole study period. \u0000Results: It has been identified that patients who were administered treatment by use of FSCs revealed improvements of their immune status. \u0000Conclusion: FSCs use in complex treatment of patients with CP stabilizes disease compensation and leads to improvement of immune blood findings.","PeriodicalId":89694,"journal":{"name":"Journal of stem cell research & therapy","volume":" ","pages":"1-6"},"PeriodicalIF":0.0,"publicationDate":"2017-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47525512","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2