Polytherapy with Reserpine and Glucagon-like Peptide-1 (GLP-1) Improves the Symptoms in Streptozotocin-Induced Type-1 Diabetic Mice by Reducing Inflammation and Inducting Beta Cell Regeneration

Abstract

The present study investigated the effects of a polytherapy with Glucagon-like Peptide-1 (GLP-1) and the Vesicular Monoamine Transporter (VMAT) inhibitor reserpine on Streptozotocin (STZ)-induced type 1 diabetic mice. Methods: Experimental diabetes was induced in C57BL/6 mice by STZ. Reserpine and GLP-1 were applied intraperitoneally as polyor monotherapy. Results and Conclusion: We show for the first time that reserpine/GLP-1 polytherapy has a hypoglycaemic effect and increases the concentrations of insulin and GLP-1, reduces the level of IL-1β in the serum and infiltration of inflammatory cells into the pancreas, led to an increased number of multipotent beta cell progenitors, oligopotent beta cell precursors and Pdx1+ beta cells. We observed increased regeneration of beta cells in the diabetic pancreas. Hence, our approach could pave the way for developing new therapy approaches for Type 1 Diabetes (T1D). Polytherapy with Reserpine and Glucagon-like Peptide-1 (GLP-1) Improves the Symptoms in Streptozotocin-Induced Type-1 Diabetic Mice by Reducing Inflammation and Inducting Beta Cell Regeneration Evgenii Germanovich Skurikhin1, Olga Victorovna Pershina1*, Natalia Nikolaevna Ermakova1, Lubov Alexandrovna Ermolaeva1, Vyacheslav Andreevich Krupin1, Angelina Vladimirovna Pakhomova1, Edgar Sergeevich Pan1, Marie-Theres Zeuner2, Darius Widera2, Ekaterina Sergeevna Khmelevskaya1, Vladimir Petrovich Fisenko3 and Alexander Mikhaylovich Dygai1 1Laboratory of Regenerative Pharmacology, Goldberg ED Research Institute of Pharmacology and Regenerative Medicine, Tomsk National Research Medical Centre of the Russian Academy of Sciences, Tomsk, Russia 2Stem Cell Biology and Regenerative Medicine Group, Department of Pharmacology, School of Pharmacy, University of Reading, Reading, United Kingdom 3Department of Pharmacology, Sechenov First State Medical University, Russia *Corresponding author: Olga Victorovna Pershina, Laboratory of Regenerative Pharmacology, Goldberg ED Research Institute of Pharmacology and Regenerative Medicine, Tomsk National Research Medical Centre of the Russian Academy of Sciences, Tomsk, Russia, Tel: 8(3822) 3822-418-374; Fax: +7-3822-418-375; E-mail: ovpershina@gmail.com Received July 20, 2018; Accepted August 14, 2018; Published August 20, 2018 Citation: Skurikhin EG, Pershina OV, Ermakova NN, Ermolaeva LA, Krupin VA, et al. (2018) Polytherapy with Reserpine and Glucagon-like Peptide-1 (GLP1) Improves the Symptoms in Streptozotocin-Induced Type-1 Diabetic Mice by Reducing Inflammation and Inducting Beta Cell Regeneration. Stem Cell Res Ther 8: 434. doi: 10.4172/2157-7633.1000434 Copyright: © 2018 Skurikhin EG, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.