Advanced biomaterial PepGel - a new tool for translational research in cell therapy

Abstract

Cancer ruins one of the utmost lethal ailments for human. Oncogenic fusion gene is one of the significant mechanisms driving the evolution of human cancers. MAN2A1-FER, a fusion gene among the mannosidase domain of MAN2A1 and tyrosine kinase domain of FER, was found in 6 diverse sorts of human malignancies. MAN2A1-FER fusion translocated FER domain from cytoplasm to golgi apparatus, and directed to phosphorylation of N-terminus of EGFR and establishment of EGFR signaling path. Expression of MAN2A1-FER produced vivid increase of development and incursion of cancers, while elimination of MAN2A1-FER through knockout created important lower level of growth and metastasis. The presence of MAN2A1-FER improved the compassion of human cancers to FER kinase inhibitor crizotinib or EGFR kinase inhibitor canertinib. Hydrodynamic tail-vein injection of MAN2A1-FER gene caused in quick expansion of liver cancer in pests with somatic Pten deletion. Taken together, we determined that MAN2A1-FER fusion gene is one of the vital drivers for human cancer development.