Biotechnology advances最新文献

{"title":"Cell-free systems: A synthetic biology tool for rapid prototyping in metabolic engineering","authors":"Kumyoung Jeung ,&nbsp;Minsun Kim ,&nbsp;Eunsoo Jang ,&nbsp;Yang Jun Shon ,&nbsp;Gyoo Yeol Jung","doi":"10.1016/j.biotechadv.2025.108522","DOIUrl":"10.1016/j.biotechadv.2025.108522","url":null,"abstract":"<div><div>Microbial cell factories provide sustainable alternatives to petroleum-based chemical production using cost-effective substrates. A deep understanding of their metabolism is essential to harness their potential along with continuous efforts to improve productivity and yield. However, the construction and evaluation of numerous genetic variants are time-consuming and labor-intensive. Cell-free systems (CFSs) serve as powerful platforms for rapid prototyping of genetic circuits, metabolic pathways, and enzyme functionality. They offer numerous advantages, including minimizing unwanted metabolic interference, precise control of reaction conditions, reduced labor, and shorter Design-Build-Test-Learn cycles. Additionally, the introduction of <em>in vitro</em> compartmentalization strategies in CFSs enables ultra-high-throughput screening in physically separated spaces, which significantly enhances prototyping efficiency. This review highlights the latest examples of using CFS to overcome prototyping limitations in living cells with a focus on rapid prototyping, particularly regarding gene regulation, enzymes, and multienzymatic reactions in bacteria. Finally, this review evaluates CFSs as a versatile prototyping platform and discusses its future applications, emphasizing its potential for producing high-value chemicals through microbial biosynthesis.</div></div>","PeriodicalId":8946,"journal":{"name":"Biotechnology advances","volume":"79 ","pages":"Article 108522"},"PeriodicalIF":12.1,"publicationDate":"2025-01-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143036647","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Plant secondary metabolites against biotic stresses for sustainable crop protection","authors":"Tanzim Jahan ,&nbsp;Md. Nurul Huda ,&nbsp;Kaixuan Zhang ,&nbsp;Yuqi He ,&nbsp;Dili Lai ,&nbsp;Namraj Dhami ,&nbsp;Muriel Quinet ,&nbsp;Md. Arfan Ali ,&nbsp;Ivan Kreft ,&nbsp;Sun-Hee Woo ,&nbsp;Milen I. Georgiev ,&nbsp;Alisdair R. Fernie ,&nbsp;Meiliang Zhou","doi":"10.1016/j.biotechadv.2025.108520","DOIUrl":"10.1016/j.biotechadv.2025.108520","url":null,"abstract":"<div><div>Sustainable agriculture practices are indispensable for achieving a hunger-free world, especially as the global population continues to expand. Biotic stresses, such as pathogens, insects, and pests, severely threaten global food security and crop productivity. Traditional chemical pesticides, while effective, can lead to environmental degradation and increase pest resistance over time. Plant-derived natural products such as secondary metabolites like alkaloids, terpenoids, phenolics, and phytoalexins offer promising alternatives due to their ability to enhance plant immunity and inhibit pest activity. Recent advances in molecular biology and biotechnology have improved our understanding of how these natural compounds function at the cellular level, activating specific plant defense through complex biochemical pathways regulated by various transcription factors (TFs) such as MYB, WRKY, bHLH, bZIP, NAC, and AP2/ERF. Advancements in multi-omics approaches, including genomics, transcriptomics, proteomics, and metabolomics, have significantly improved the understanding of the regulatory networks that govern PSM synthesis. These integrative approaches have led to the discovery of novel insights into plant responses to biotic stresses, identifying key regulatory genes and pathways involved in plant defense. Advanced technologies like CRISPR/Cas9-mediated gene editing allow precise manipulation of PSM pathways, further enhancing plant resistance. Understanding the complex interaction between PSMs, TFs, and biotic stress responses not only advances our knowledge of plant biology but also provides feasible strategies for developing crops with improved resistance to pests and diseases, contributing to sustainable agriculture and food security. This review emphasizes the crucial role of PSMs, their biosynthetic pathways, the regulatory influence of TFs, and their potential applications in enhancing plant defense and sustainability. It also highlights the astounding potential of multi-omics approaches to discover gene functions and the metabolic engineering of genes associated with secondary metabolite biosynthesis. Taken together, this review provides new insights into research opportunities for enhancing biotic stress tolerance in crops through utilizing plant secondary metabolites.</div></div>","PeriodicalId":8946,"journal":{"name":"Biotechnology advances","volume":"79 ","pages":"Article 108520"},"PeriodicalIF":12.1,"publicationDate":"2025-01-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143036648","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Electrode functional microorganisms in bioelectrochemical systems and its regulation: A review","authors":"Juping You ,&nbsp;Lei Ye ,&nbsp;Shihan Zhang ,&nbsp;Jingkai Zhao ,&nbsp;Yan Zhao ,&nbsp;Yaxue He ,&nbsp;Jianmeng Chen ,&nbsp;Christian Kennes ,&nbsp;Dongzhi Chen","doi":"10.1016/j.biotechadv.2025.108521","DOIUrl":"10.1016/j.biotechadv.2025.108521","url":null,"abstract":"<div><div>Bioelectrochemical systems (BES) as environmental remediation biotechnologies have boomed in the last two decades. Although BESs combined technologies with electro-chemistry, −biology, and -physics, microorganisms and biofilms remain at their core. In this review, various functional microorganisms in BESs for CO₂ reduction, dehalogenation, nitrate, phosphate, and sulfate reduction, metal removal, and volatile organic compound oxidation are summarized and compared in detail. Moreover, interrelationship regulation approaches for functional microorganisms and methods for electroactive biofilm development, such as targeted electrode surface modification, chemical treatment, physical revealing, biological optimization, and genetic programming are pointed out. This review provides promising guidance and suggestions for the selection of microbial inoculants and provides an analysis of the role of individual microorganisms in mixed microbial communities and its metabolisms.</div></div>","PeriodicalId":8946,"journal":{"name":"Biotechnology advances","volume":"79 ","pages":"Article 108521"},"PeriodicalIF":12.1,"publicationDate":"2025-01-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142999305","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Advancements of astaxanthin production in Haematococcus pluvialis: Update insight and way forward","authors":"Hongli Cui ,&nbsp;Xiaoli Zhu ,&nbsp;Xiao Yu ,&nbsp;Siming Li ,&nbsp;Kang Wang ,&nbsp;Le Wei ,&nbsp;Runzhi Li ,&nbsp;Song Qin","doi":"10.1016/j.biotechadv.2025.108519","DOIUrl":"10.1016/j.biotechadv.2025.108519","url":null,"abstract":"<div><div>The global market demand for natural astaxanthin (AXT) is growing rapidly owing to its potential human health benefits and diverse industry applications, driven by its safety, unique structure, and special function. Currently, the alga <em>Haematococcus pluvialis</em> (alternative name <em>H. lacustris</em>) has been considered as one of the best large-scale producers of natural AXT. However, the industry's further development faces two main challenges: the limited cultivation areas due to light-dependent AXT accumulation and the low AXT yield coupled with high production costs resulting from complex, time-consuming upstream biomass culture and downstream AXT extraction processes. Therefore, it is urgently to develop novel strategies to improve the AXT production in <em>H. pluvialis</em> to meet industrial demands, which makes its commercialization cost-effective. Although several strategies related to screening excellent target strains, optimizing culture condition for high biomass yield, elucidating the AXT biosynthetic pathway, and exploiting effective inducers for high AXT content have been applied to enhance the AXT production in <em>H. pluvialis</em>, there are still some unsolved and easily ignored perspectives. In this review, firstly, we summarize the structure and function of natural AXT focus on those from the algal <em>H. pluvialis</em>. Secondly, the latest findings regarding the AXT biosynthetic pathway including spatiotemporal specificity, transport, esterification, and storage are updated. Thirdly, we systematically assess enhancement strategies on AXT yield. Fourthly, the regulation mechanisms of AXT accumulation under various stresses are discussed. Finally, the integrated and systematic solutions for improving AXT production are proposed. This review not only fills the existing gap about the AXT accumulation, but also points the way forward for AXT production in <em>H. pluvialis.</em></div></div>","PeriodicalId":8946,"journal":{"name":"Biotechnology advances","volume":"79 ","pages":"Article 108519"},"PeriodicalIF":12.1,"publicationDate":"2025-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142969467","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Recent advances in synthetic biology toolkits and metabolic engineering of Ralstonia eutropha H16 for production of value-added chemicals","authors":"Ye Wang ,&nbsp;Yao Tian ,&nbsp;Dake Xu ,&nbsp;Shaoan Cheng ,&nbsp;Wen-Wei Li ,&nbsp;Hao Song","doi":"10.1016/j.biotechadv.2025.108516","DOIUrl":"10.1016/j.biotechadv.2025.108516","url":null,"abstract":"<div><div><em>Ralstonia eutropha</em> H16, a facultative chemolithoautotrophic Gram-negative bacterium, demonstrates remarkable metabolic flexibility by utilizing either diverse organic substrates or CO₂ as the sole carbon source, with H₂ serving as the electron donor under aerobic conditions. The capacity of carbon and energy metabolism of <em>R. eutropha</em> H16 enabled development of synthetic biology technologies and strategies to engineer its metabolism for biosynthesis of value-added chemicals. This review firstly outlines the development of synthetic biology tools tailored for <em>R. eutropha</em> H16, including construction of expression vectors, regulatory elements, and transformation techniques. The availability of comprehensive omics data (i.e., transcriptomic, proteomic, and metabolomic) combined with the fully annotated genome sequence provides a robust genetic framework for advanced metabolic engineering. These advancements facilitate efficient reprogramming metabolic network of <em>R. eutropha</em>. The potential of <em>R. eutropha</em> as a versatile microbial platform for industrial biotechnology is further underscored by its ability to utilize a wide range of carbon sources for the production of value-added chemicals through both autotrophic and heterotrophic pathways. The integration of state-of-the-art genetic and genomic engineering tools and strategies with high cell-density fermentation processes enables engineered <em>R. eutropha</em> as promising microbial cell factories for optimizing carbon fluxes and expanding the portfolio of bio-based products.</div></div>","PeriodicalId":8946,"journal":{"name":"Biotechnology advances","volume":"79 ","pages":"Article 108516"},"PeriodicalIF":12.1,"publicationDate":"2025-01-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142963685","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Advancement in synthetic gene circuits engineering: An alternative strategy for microRNA imaging and disease theranostics","authors":"Kulsoom ,&nbsp;Wajahat Ali ,&nbsp;Fu Wang","doi":"10.1016/j.biotechadv.2025.108518","DOIUrl":"10.1016/j.biotechadv.2025.108518","url":null,"abstract":"<div><div>Gene circuits, which are genetically engineered systems designed to regulate gene expression, are emerging as powerful tools in disease theranostics, especially in mammalian cells. This review explores the latest advances in the design and application of gene circuits for detecting and treating various diseases. Synthetic gene circuits, inspired by electronic systems, offer precise control over therapeutic gene activity, allowing for real-time, user-defined responses to pathological signals. Notable applications include synZiFTRs for T-cell-based cancer therapies, immunomagnetic circuits for combating antibiotic-resistant infections like MRSA, and caffeine-induced circuits for managing type-2 diabetes. Additionally, advanced designs such as TetR-Elk1 circuits for reversing insulin resistance, RNAi circuits for targeting cancer cells, and synthetic circuits for managing metabolic conditions like urate homeostasis and diet-induced obesity are highlighted. These gene circuits, tailored for mammalian cells, showcase immense potential in gene- and cell-based therapies for complex metabolic and immune-related disorders, paving the way for precise, customizable treatments. The review focuses on the use of these circuits in mammalian systems and emphasizes their therapeutic implications, offering insights into future developments in disease treatment.</div></div>","PeriodicalId":8946,"journal":{"name":"Biotechnology advances","volume":"79 ","pages":"Article 108518"},"PeriodicalIF":12.1,"publicationDate":"2025-01-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142969466","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum to “Recent advances in enzyme-enhanced immunosensors” [Biotechnology Advances 53 (2021) 107867].","authors":"Yanna Shao ,&nbsp;Huan Zhou ,&nbsp;Qingping Wu ,&nbsp;Yonghua Xiong ,&nbsp;Juan Wang ,&nbsp;Yu Ding","doi":"10.1016/j.biotechadv.2025.108515","DOIUrl":"10.1016/j.biotechadv.2025.108515","url":null,"abstract":"","PeriodicalId":8946,"journal":{"name":"Biotechnology advances","volume":"79 ","pages":"Article 108515"},"PeriodicalIF":12.1,"publicationDate":"2025-01-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142944043","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mycelium-based composites: An updated comprehensive overview","authors":"Emma Camilleri ,&nbsp;Sumesh Narayan ,&nbsp;Divnesh Lingam ,&nbsp;Renald Blundell","doi":"10.1016/j.biotechadv.2025.108517","DOIUrl":"10.1016/j.biotechadv.2025.108517","url":null,"abstract":"<div><div>Mycelium-based composites hold significant potential as sustainable alternatives to traditional materials, offering innovative solutions to the escalating challenges of global warming and climate change. This review examines their production techniques, advantages, and limitations, emphasizing their role in addressing pressing environmental and economic concerns. Current applications span various industries, including manufacturing and biomedical fields, where mycelium-based composites demonstrate the capacity to mitigate environmental impact and enhance economic sustainability. Key findings highlight their environmental benefits, economic viability, and versatile applications, showcasing their potential to revolutionize multiple sectors. However, challenges such as consumer acceptance, intrinsic variability, and the need for standardized guidelines persist, underscoring the importance of further research and innovation. By optimizing material properties and refining production processes, mycelium-based composites could pave the way for widespread adoption as sustainable materials, contributing to a greener and more environmentally conscious future.</div></div>","PeriodicalId":8946,"journal":{"name":"Biotechnology advances","volume":"79 ","pages":"Article 108517"},"PeriodicalIF":12.1,"publicationDate":"2025-01-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142939777","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chiral helical scaffolds: Unlocking their potential in biomolecular interactions and biomedical applications","authors":"Ghada Bouz ,&nbsp;Jaroslav Žádný ,&nbsp;Jan Storch ,&nbsp;Jan Vacek","doi":"10.1016/j.biotechadv.2024.108513","DOIUrl":"10.1016/j.biotechadv.2024.108513","url":null,"abstract":"<div><div>In nature, various molecules possess spiral geometry. Such helical structures are even prevalent within the human body, represented classically by DNA and three-dimensional (secondary structure) protein folding. In this review, we chose helicenes and helicene-like structures –synthetically accessible carbon-rich molecules– as a compelling example of helically chiral scaffolds. Helicene chemistry, traditionally anchored in materials science, has been a subject of increasing interest in the biomedical field due to the unique optical and chiral properties of these helical structures. This review explores the diverse applications of helicenes in biomedicine, focusing on their role in cell imaging, protective coatings for implants, drug delivery systems, biosensors, and drug discovery. We discuss the unique properties of helicenes and helicene-like structures, highlighting their ability to form complex interactions with various biomolecules and their potential in the development of candidates for therapeutic agents. Recent advances in helicene derivatives with enhanced circularly polarized luminescence and other photochemical properties are also reviewed, underlining their utility in precise bio-imaging and diagnostic techniques. The review consolidates the current literature and emphasizes the growing importance of helicenes in bridging chemistry, materials science, and biology for innovative technological and biomedical applications.</div></div>","PeriodicalId":8946,"journal":{"name":"Biotechnology advances","volume":"79 ","pages":"Article 108513"},"PeriodicalIF":12.1,"publicationDate":"2025-01-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142930580","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Advances in bacterial glycoprotein engineering: A critical review of current technologies, emerging challenges, and future directions","authors":"Ziyu Li ,&nbsp;Yujie Wang ,&nbsp;Xiaojing Zhao ,&nbsp;Qing Meng ,&nbsp;Guozhen Ma ,&nbsp;Lijie Xie ,&nbsp;Xiaolong Jiang ,&nbsp;Yutao Liu ,&nbsp;Di Huang","doi":"10.1016/j.biotechadv.2024.108514","DOIUrl":"10.1016/j.biotechadv.2024.108514","url":null,"abstract":"<div><div>Protein glycosylation, which involves the addition of carbohydrate chains to amino acid side chains, imparts essential properties to proteins, offering immense potential in synthetic biology applications. Despite its importance, natural glycosylation pathways present several limitations, highlighting the need for new tools to better understand glycan structures, recognition, metabolism, and biosynthesis, and to facilitate the production of biologically relevant glycoproteins. The field of bacterial glycoengineering has gained significant attention due to the ongoing discovery and study of bacterial glycosylation systems. By utilizing protein glycan coupling technology, a wide range of valuable glycoproteins for clinical and diagnostic purposes have been successfully engineered. This review outlines the recent advances in bacterial protein glycosylation from the perspective of synthetic biology and metabolic engineering, focusing on the development of new glycoprotein therapeutics and vaccines. We provide an overview of the production of high-value, customized glycoproteins using prokaryotic glycosylation platforms, with particular emphasis on four key elements: (i) glycosyltransferases, (ii) carrier proteins, (iii) glycosyl donors, and (iv) host bacteria. Optimization of these elements enables precise control over glycosylation patterns, thus enhancing the potential of the resulting products. Finally, we discuss the challenges and future prospects of leveraging synthetic biology technologies to develop microbial glyco-factories and cell-free systems for efficient glycoprotein production.</div></div>","PeriodicalId":8946,"journal":{"name":"Biotechnology advances","volume":"79 ","pages":"Article 108514"},"PeriodicalIF":12.1,"publicationDate":"2025-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142925973","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}