Biotechnology advances最新文献

{"title":"Metagenomics and plant-microbe symbioses: Microbial community dynamics, functional roles in carbon sequestration, nitrogen transformation, sulfur and phosphorus mobilization for sustainable soil health","authors":"Atif Khurshid Wani ,&nbsp;Fayzan Qadir ,&nbsp;Noureddine Elboughdiri ,&nbsp;Farida Rahayu ,&nbsp;Saefudin ,&nbsp;Dibyo Pranowo ,&nbsp;Chaireni Martasari ,&nbsp;Mia Kosmiatin ,&nbsp;Cece Suhara ,&nbsp;Tri Sudaryono ,&nbsp;Yusmani Prayogo ,&nbsp;Krishna Kumar Yadav ,&nbsp;Khursheed Muzammil ,&nbsp;Lienda Bashier Eltayeb ,&nbsp;Maha Awjan Alreshidi ,&nbsp;Reena Singh","doi":"10.1016/j.biotechadv.2025.108580","DOIUrl":"10.1016/j.biotechadv.2025.108580","url":null,"abstract":"<div><div>Biogeochemical cycles are fundamental processes that regulate the flow of essential elements such as carbon, nitrogen, and phosphorus, sustaining ecosystem productivity and global biogeochemical equilibrium. These cycles are intricately influenced by plant-microbe symbioses, which facilitate nutrient acquisition, organic matter decomposition, and the transformation of soil nutrients. Through mutualistic interactions, plants and microbes co-regulate nutrient availability and promote ecosystem resilience, especially under environmental stress. Metagenomics has emerged as a transformative tool for deciphering the complex microbial communities and functional genes driving these cycles. By enabling the high-throughput sequencing and annotation of microbial genomes, metagenomics provides unparalleled insights into the taxonomic diversity, metabolic potential, and functional pathways underlying microbial contributions to biogeochemical processes. Unlike previous reviews, this work integrates recent advancements in metagenomics with complementary omics approaches to provide a comprehensive perspective on how plant-microbe interactions modulate biogeochemical cycles at molecular, genetic, and ecosystem levels. By highlighting novel microbial processes and potential biotechnological applications, this review aims to guide future research in leveraging plant-microbe symbioses for sustainable agriculture, ecosystem restoration, and climate change mitigation.</div></div>","PeriodicalId":8946,"journal":{"name":"Biotechnology advances","volume":"82 ","pages":"Article 108580"},"PeriodicalIF":12.1,"publicationDate":"2025-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143838789","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synthetic biology of Fusarium for the sustainable production of valuable bioproducts","authors":"Rima Gnaim ,&nbsp;Rodrigo Ledesma-Amaro","doi":"10.1016/j.biotechadv.2025.108579","DOIUrl":"10.1016/j.biotechadv.2025.108579","url":null,"abstract":"<div><div>Synthetic biology offers transformative opportunities to optimise <em>Fusarium</em> species as efficient platforms for the sustainable production of diverse bioproducts. Advanced engineering techniques, including CRISPR/Cas9, RNA interference and synthetic promoters, have enhanced the manipulation of metabolic pathways, enabling higher yields of industrially relevant compounds. Recent insights from next-generation sequencing and omics technologies have significantly expanded our understanding of <em>Fusarium's</em> metabolic networks, leading to more precise strain engineering. Despite these advances, challenges such as metabolic bottlenecks, regulatory complexities and strain stability remain significant barriers to industrial-scale applications. The development of efficient genetic tools, together with the expansion of our knowledge of <em>Fusarium</em> physiology and genetics thanks to systems biology approaches, holds promise to unlock <em>Fusarium's</em> full potential as a sustainable cell factory. This review focuses on the genetic and metabolic tools available to enhance <em>Fusarium's</em> capacity to produce biofuels, pharmaceuticals, enzymes and other valuable compounds. It also highlights key innovations and discusses future directions for leveraging <em>Fusarium</em> as an environmentally friendly bioproduction system.</div></div>","PeriodicalId":8946,"journal":{"name":"Biotechnology advances","volume":"81 ","pages":"Article 108579"},"PeriodicalIF":12.1,"publicationDate":"2025-04-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143825621","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biotechnological production and emerging applications of betalains: A review","authors":"Mohammad Imtiyaj Khan ,&nbsp;Guy Polturak","doi":"10.1016/j.biotechadv.2025.108576","DOIUrl":"10.1016/j.biotechadv.2025.108576","url":null,"abstract":"<div><div>Betalains are food-grade hydrophilic pigments with antioxidant and biological activities, predominantly found in plants. Betanin is a red-violet betalain synthesized from tyrosine through L-DOPA formation, its subsequent aromatic ring-opening, spontaneous cyclization to betalamic acid, and then pH-dependent condensation with i) cyclo‐DOPA-5-<em>O</em>-glucoside or ii) cyclo‐DOPA followed by 5-<em>O</em>-glucosylation. This short pathway in plants for betanin biosynthesis has been heterologously expressed in other organisms (e.g. non-betalainic plants, yeasts, and fungi) using <em>CYP76AD1</em>, <em>DOD1</em>, and <em>cDOPA5GT</em> or <em>B5GT</em>, corresponding to the enzymatic steps mentioned above. For the red-violet color formation through heterologous expression of the pathway genes in non-betalainic plants, a simplified reporter gene called <em>RUBY</em> has been developed recently. Without any systems engineering, expression of <em>RUBY</em> in non-betalainic plants resulted in accumulation of up to 203 mg betalains/100 g fresh weight of peanut leaves. In yeasts, <em>Saccharomyces cerevisiae</em> and <em>Yarrowia lipolytica</em>, and fungus <em>Fusarium venenatum</em>, betanin production has been achieved through overexpression of the pathway genes, with productivity reaching up to 0.62 mg/L/h, 26 mg/L/h, and 26.4 mg/L/h from <span>d</span>-glucose as carbon source, respectively, after considerable systems engineering and gene copy number augmentation. This review critically analyzes recent biotechnological production of betalains to highlight the advancements and strategies for improvement in the technology. Also, emerging applications of betalain biosynthetic gene products or betalains as biosensors, fluorescent probes, meat analog colors, and others are discussed to strengthen the need for systems engineering and process optimization for large-scale industrial production of these pigments.</div></div>","PeriodicalId":8946,"journal":{"name":"Biotechnology advances","volume":"81 ","pages":"Article 108576"},"PeriodicalIF":12.1,"publicationDate":"2025-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143815023","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fungal ergot alkaloids: Metabolic pathways, biological functions, and advances in synthetic reprogramming","authors":"Wanling Jiang ,&nbsp;Xingyu Hou ,&nbsp;Guoxiong Peng ,&nbsp;Yuxian Xia ,&nbsp;Yueqing Cao","doi":"10.1016/j.biotechadv.2025.108578","DOIUrl":"10.1016/j.biotechadv.2025.108578","url":null,"abstract":"<div><div>Ergot alkaloids (EAs) are a class of secondary metabolites produced by fungi. These compounds are predominantly synthesized by Ascomycota, with variations in types and biosynthetic pathways among different fungal species. The EA synthesis has minimal impact on the normal growth and development of most EA-producing fungi, but serves as a virulence factor that influences the biocontrol functions of entomopathogenic fungi and symbiotic fungi in plants. In the medical field, EAs have been widely used for treating neurological disorders such as Parkinson's disease. However, the biosynthetic pathways of EAs are highly complex and significantly influenced by environmental factors, resulting in low yields from field production or chemical synthesis. To address the global demand for EAs, various strategies have been developed to reprogram the biosynthetic pathways in some chassis strains, aiming to simplify the process and increase EA production. This review summarizes the biosynthetic pathways and regulatory mechanisms of EAs in fungi, their biological functions, and recent advances in strategies for synthetic reprogramming.</div></div>","PeriodicalId":8946,"journal":{"name":"Biotechnology advances","volume":"81 ","pages":"Article 108578"},"PeriodicalIF":12.1,"publicationDate":"2025-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143808059","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Polyethylene biodegradation: A multifaceted approach","authors":"Anjali Purohit ,&nbsp;Bastien Cochereau ,&nbsp;Omprakash Sarkar ,&nbsp;Ulrika Rova ,&nbsp;Paul Christakopoulos ,&nbsp;Io Antonopoulou ,&nbsp;Silas Villas-Boas ,&nbsp;Leonidas Matsakas","doi":"10.1016/j.biotechadv.2025.108577","DOIUrl":"10.1016/j.biotechadv.2025.108577","url":null,"abstract":"<div><div>The inert nature, durability, low cost, and wide applicability of plastics have made this material indispensable in our lives. This dependency has resulted in a growing number of plastic items, of which a substantial part is disposed in landfills or dumped in the environment, thereby affecting terrestrial and aquatic ecosystems. Among plastic materials, polyolefins are the most abundant and are impervious to biodegradation, owing to the presence of strong C<img>C and C<img>H bonds. Nevertheless, naturally occurring biodegradation of polyolefins, albeit limited, has been reported. This observation has sparked research on microbial polyolefin degradation. More efficient and targeted versions of this process could be developed also in the laboratory by designing synthetic microbial consortia with engineered enzymes. In this review, we discuss strategies for the development of such microbial consortia and identification of novel polyolefin-degrading microorganisms, as well as the engineering of polyethylene-oxidizing enzymes with greater catalytic efficacy. Finally, different techniques for the design of synthetic microbial consortia capable of successful polyolefin bioremediation will be outlined.</div></div>","PeriodicalId":8946,"journal":{"name":"Biotechnology advances","volume":"82 ","pages":"Article 108577"},"PeriodicalIF":12.1,"publicationDate":"2025-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143787693","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Taking the 3Rs to a higher level: replacement and reduction of animal testing in life sciences in space research","authors":"Mathieu Vinken ,&nbsp;Daniela Grimm ,&nbsp;Sarah Baatout ,&nbsp;Bjorn Baselet ,&nbsp;Afshin Beheshti ,&nbsp;Markus Braun ,&nbsp;Anna Catharina Carstens ,&nbsp;James A. Casaletto ,&nbsp;Ben Cools ,&nbsp;Sylvain V. Costes ,&nbsp;Phoebe De Meulemeester ,&nbsp;Bartu Doruk ,&nbsp;Sara Eyal ,&nbsp;Miguel J.S. Ferreira ,&nbsp;Silvana Miranda ,&nbsp;Christiane Hahn ,&nbsp;Sinem Helvacıoğlu Akyüz ,&nbsp;Stefan Herbert ,&nbsp;Dmitriy Krepkiy ,&nbsp;Yannick Lichterfeld ,&nbsp;Danilo A. Tagle","doi":"10.1016/j.biotechadv.2025.108574","DOIUrl":"10.1016/j.biotechadv.2025.108574","url":null,"abstract":"<div><div>Human settlements on the Moon, crewed missions to Mars and space tourism will become a reality in the next few decades. Human presence in space, especially for extended periods of time, will therefore steeply increase. However, despite more than 60 years of spaceflight, the mechanisms underlying the effects of the space environment on human physiology are still not fully understood. Animals, ranging in complexity from flies to monkeys, have played a pioneering role in understanding the (patho)physiological outcome of critical environmental factors in space, in particular altered gravity and cosmic radiation. The use of animals in biomedical research is increasingly being criticized because of ethical reasons and limited human relevance. Driven by the 3Rs concept, calling for replacement, reduction and refinement of animal experimentation, major efforts have been focused in the past decades on the development of alternative methods that fully bypass animal testing or so-called new approach methodologies. These new approach methodologies range from simple monolayer cultures of individual primary or stem cells all up to bioprinted 3D organoids and microfluidic chips that recapitulate the complex cellular architecture of organs. Other approaches applied in life sciences in space research contribute to the reduction of animal experimentation. These include methods to mimic space conditions on Earth, such as microgravity and radiation simulators, as well as tools to support the processing, analysis or application of testing results obtained in life sciences in space research, including systems biology, live-cell, high-content and real-time analysis, high-throughput analysis, artificial intelligence and digital twins. The present paper provides an in-depth overview of such methods to replace or reduce animal testing in life sciences in space research.</div></div>","PeriodicalId":8946,"journal":{"name":"Biotechnology advances","volume":"81 ","pages":"Article 108574"},"PeriodicalIF":12.1,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143778890","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Host cell protein-mediated adjuvanticity and immunogenicity risks of biotherapeutics","authors":"Sherin Panikulam ,&nbsp;Hannah Morgan ,&nbsp;Michael Gutknecht ,&nbsp;Thomas K. Villiger ,&nbsp;Nicolas Lebesgue ,&nbsp;Anette C. Karle","doi":"10.1016/j.biotechadv.2025.108575","DOIUrl":"10.1016/j.biotechadv.2025.108575","url":null,"abstract":"<div><div>Host cell proteins (HCPs) are process-related impurities of biotherapeutic production that might affect product quality and/or patient safety. In a few cases, adverse events were attributed to HCPs present in the administered biotherapeutic. HCP-associated immune risks include adjuvanticity and immunogenicity with potential cross-reactivity. Based on the published data, some HCPs can act as adjuvants increasing the immunogenicity of the biotherapeutic as a bystander effect. HCPs may also induce immunogenicity against themselves, resulting in anti-HCP T cell responses and anti-HCP antibody formation. Depending on sequence similarities, these anti-HCP immune responses might theoretically be cross-reactive to the biotherapeutic or human endogenous proteins. In this review, we examine HCP-associated immune-related risks reported from non-clinical and clinical studies. We also discuss the potential and limitations of <em>in vitro</em> and <em>in silico</em> methods to evaluate the adjuvanticity and immunogenicity potential of HCPs. A risk-based assessment of the safety impact of HCPs may include the identity of the HCP and similarity to the biotherapeutic and human proteins, as well as product, treatment-, and patient-related factors.</div></div>","PeriodicalId":8946,"journal":{"name":"Biotechnology advances","volume":"81 ","pages":"Article 108575"},"PeriodicalIF":12.1,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143778888","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genetic mechanisms, biological function, and biotechnological advance in sorghum tannins research","authors":"Fu Wang ,&nbsp;Qian Zhao ,&nbsp;Shuyao Li ,&nbsp;Ruidong Sun ,&nbsp;Zhenyuan Zang ,&nbsp;Ai-sheng Xiong ,&nbsp;El Hadji Moussa Seck ,&nbsp;Yuxin Ye ,&nbsp;Jian Zhang","doi":"10.1016/j.biotechadv.2025.108573","DOIUrl":"10.1016/j.biotechadv.2025.108573","url":null,"abstract":"<div><div>Sorghum (<em>Sorghum bicolor</em>) holds a unique position in the human diet and serves as a stable food source in many developing countries especially in African and south Asian regions. Tannins, the primary secondary metabolites in sorghum, are pivotal in determining its characteristic bitter taste. Beyond their influence on flavor, tannins play a vital role in sorghum's resistance to biotic and abiotic stresses and serve as key indicators of grain quality. The concentration of tannins significantly affects the potential for diverse applications of sorghum. This review provides a comprehensive analysis of sorghum tannins, focusing on their genetic basis, biological activities, and biosynthesis mechanisms. It highlights the relationship between tannin levels and grain color and delves into the underlying biogenetic pathways. Furthermore, the potential of functional genomics and biotechnological approaches in precisely controlling tannin levels for sorghum breeding is discussed. This study aims to offer valuable insights and perspectives for advancing both the scientific understanding and practical applications of sorghum tannins.</div></div>","PeriodicalId":8946,"journal":{"name":"Biotechnology advances","volume":"81 ","pages":"Article 108573"},"PeriodicalIF":12.1,"publicationDate":"2025-03-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143760555","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Application of curcuminoids in inflammatory, neurodegenerative and aging conditions - Pharmacological potential and bioengineering approaches to improve efficiency","authors":"Ricardo Lagoa ,&nbsp;Logesh Rajan ,&nbsp;Cristiana Violante ,&nbsp;Smith B. Babiaka ,&nbsp;Dorinda Marques-da-Silva ,&nbsp;Bhupinder Kapoor ,&nbsp;Flávio Reis ,&nbsp;Atanas G. Atanasov","doi":"10.1016/j.biotechadv.2025.108568","DOIUrl":"10.1016/j.biotechadv.2025.108568","url":null,"abstract":"<div><div>Curcumin, a natural compound found in turmeric, has shown promise in treating brain-related diseases and conditions associated with aging. Curcumin has shown multiple anti-inflammatory and brain-protective effects, but its clinical use is limited by challenges like poor absorption, specificity and delivery to the right tissues.</div><div>A range of contemporary approaches at the intersection with bioengineering and systems biology are being explored to address these challenges. Data from preclinical and human studies highlight various neuroprotective actions of curcumin, including the inhibition of neuroinflammation, modulation of critical cellular signaling pathways, promotion of neurogenesis, and regulation of dopamine levels. However, curcumin's multifaceted effects - such as its impact on microRNAs and senescence markers - suggest novel therapeutic targets in neurodegeneration. Tetrahydrocurcumin, a primary metabolite of curcumin, also shows potential due to its presence in circulation and its anti-inflammatory properties, although further research is needed to elucidate its neuroprotective mechanisms.</div><div>Recent advancements in delivery systems, particularly brain-targeting nanocarriers like polymersomes, micelles, and liposomes, have shown promise in enhancing curcumin's bioavailability and therapeutic efficacy in animal models. Furthermore, the exploration of drug-laden scaffolds and dermal delivery may extend the pharmacological applications of curcumin. Studies reviewed here indicate that engineered dermal formulations and devices could serve as viable alternatives for neuroprotective treatments and to manage skin or musculoskeletal inflammation. This work highlights the need for carefully designed, long-term studies to better understand how curcumin and its bioactive metabolites work, their safety, and their effectiveness.</div></div>","PeriodicalId":8946,"journal":{"name":"Biotechnology advances","volume":"82 ","pages":"Article 108568"},"PeriodicalIF":12.1,"publicationDate":"2025-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143742020","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antimicrobial peptides in nematode secretions – Unveiling biotechnological opportunities for therapeutics and beyond","authors":"E.I. Sutcliffe ,&nbsp;A. Irvine ,&nbsp;J. Rooney ,&nbsp;D. Smith ,&nbsp;H.M. Northcote ,&nbsp;D. McKenzie ,&nbsp;S. Bakshi ,&nbsp;A.J. Nisbet ,&nbsp;D. Price ,&nbsp;R. Graham ,&nbsp;R. Morphew ,&nbsp;L. Atkinson ,&nbsp;A. Mousley ,&nbsp;C. Cantacessi","doi":"10.1016/j.biotechadv.2025.108572","DOIUrl":"10.1016/j.biotechadv.2025.108572","url":null,"abstract":"<div><div>Gastrointestinal (GI) parasitic nematodes threaten food security and affect human health and animal welfare globally. Current anthelmintics for use in humans and livestock are challenged by continuous re-infections and the emergence and spread of multidrug resistance, underscoring an urgent need to identify novel control targets for therapeutic exploitation. Recent evidence has highlighted the occurrence of complex interplay between GI parasitic nematodes of humans and livestock and the resident host gut microbiota. Antimicrobial peptides (AMPs) found within nematode biofluids have emerged as potential effectors of these interactions. This review delves into the occurrence, structure, and function of nematode AMPs, highlighting their potential as targets for drug discovery and development. We argue that an integrated approach combining advanced analytical techniques, scalable production methods, and innovative experimental models is needed to unlock the full potential of nematode AMPs and pave the way for the discovery and development of sustainable parasite control strategies.</div></div>","PeriodicalId":8946,"journal":{"name":"Biotechnology advances","volume":"81 ","pages":"Article 108572"},"PeriodicalIF":12.1,"publicationDate":"2025-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143742019","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}