Biotechnology advances最新文献

Mild hypothermia: Insights and implications for productivity in mammalian and insect cell cultures 亚低温：对哺乳动物和昆虫细胞培养生产力的见解和影响

IF 16 1区工程技术

Biotechnology advances Pub Date : 2025-06-11 DOI: 10.1016/j.biotechadv.2025.108625

Dayana Morales Borrell, Ziomara P. Gerdtzen

{"title":"Mild hypothermia: Insights and implications for productivity in mammalian and insect cell cultures","authors":"Dayana Morales Borrell, Ziomara P. Gerdtzen","doi":"10.1016/j.biotechadv.2025.108625","DOIUrl":"https://doi.org/10.1016/j.biotechadv.2025.108625","url":null,"abstract":"Mammalian cells are the preferred expression system for obtaining recombinant proteins (r-proteins) due to their ability to generate human-like glycosylation patterns. However, their slow growth and lower productivity compared to prokaryotic host cells, coupled with the rising demand for complex therapeutic proteins in the biopharmaceutical market, have driven the search for alternatives to boost productivity. In this context, mild hypothermia (MH) has emerged as a valuable tool for enhancing both the viability and productivity of r-proteins in mammalian as well as some insect-derived cell lines. Notably, the impact of MH varies depending on the r-protein and cell line, and is influenced by factors such as promoter type, temperature reduction methods, supplementation, pH, and operational conditions. MH can affect substrate synthesis, toxic metabolite production, and post-translational modifications of r-proteins, particularly glycosylation. At the molecular level, MH influences processes such as cell cycle arrest, apoptosis delay, mRNA stability, protein synthesis, cytoskeletal reorganization, and the induction of endogenous transcription factors, all of which can contribute to increased productivity and viability in cell cultures. This review addresses key considerations regarding the application of MH in mammalian and insect cell cultures and provides a comprehensive overview of the molecular mechanisms underlying its effects. It also identifies potential targets for cell engineering that could further enhance r-protein production.","PeriodicalId":8946,"journal":{"name":"Biotechnology advances","volume":"41 1","pages":""},"PeriodicalIF":16.0,"publicationDate":"2025-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144289820","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fungal bioconversion of lignin-derived aromatics: Pathways, enzymes, and biotechnological potential 木质素衍生芳香化合物的真菌生物转化：途径、酶和生物技术潜力

IF 12.1 1区工程技术

Biotechnology advances Pub Date : 2025-06-10 DOI: 10.1016/j.biotechadv.2025.108624

Jinyang Li, Changyu Pi, Jintong Zhang, Fangting Jiang, Tongtong Bao, Le Gao, Xin Wu

{"title":"Fungal bioconversion of lignin-derived aromatics: Pathways, enzymes, and biotechnological potential","authors":"Jinyang Li, Changyu Pi, Jintong Zhang, Fangting Jiang, Tongtong Bao, Le Gao, Xin Wu","doi":"10.1016/j.biotechadv.2025.108624","DOIUrl":"10.1016/j.biotechadv.2025.108624","url":null,"abstract":"<div><div>Lignin, the most abundant aromatic biopolymer on Earth, holds immense potential as a renewable feedstock for the production of high-value bioproducts. However, its structural complexity and recalcitrance pose significant challenges for efficient valorization. Fungal biodegradation offers a sustainable strategy for lignin conversion by employing extracellular oxidative enzymes and specialized metabolic pathways to transform lignin-derived aromatic compounds into central metabolites or valuable end products. Despite extensive research on fungal ligninolytic systems, a systematic integration of aromatic catabolic pathways remains fragmented. This review consolidates recent advances in fungal metabolism of key lignin-derived aromatics, including cinnamic acid, gallic acid, ferulic acid and vanillin, with a focus on their assimilation into central metabolic networks and the enzymatic machinery involved. We highlight the critical role of fungal transporter systems in mediating aromatic compound uptake and efflux. Furthermore, we discuss future research directions, emphasizing the integration of synthetic biology, computational modeling, and systems biology to engineer robust fungal chassis for lignin valorization. Addressing these knowledge gaps will advance the development of fungal-based platforms for sustainable production of renewable aromatics, thereby contributing to the circular bioeconomy and green biomanufacturing.</div></div>","PeriodicalId":8946,"journal":{"name":"Biotechnology advances","volume":"83 ","pages":"Article 108624"},"PeriodicalIF":12.1,"publicationDate":"2025-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144270380","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Methanol metabolism in synthetic methylotrophic microorganisms. 合成甲基营养微生物的甲醇代谢。

IF 12.1 1区工程技术

Biotechnology advances Pub Date : 2025-06-09 DOI: 10.1016/j.biotechadv.2025.108623

Xiu Zang, Yankun Yang, Chunjun Zhan, Zhonghu Bai

{"title":"Methanol metabolism in synthetic methylotrophic microorganisms.","authors":"Xiu Zang, Yankun Yang, Chunjun Zhan, Zhonghu Bai","doi":"10.1016/j.biotechadv.2025.108623","DOIUrl":"https://doi.org/10.1016/j.biotechadv.2025.108623","url":null,"abstract":"<p><p>Methanol has garnered increasing attention as an ideal carbon source for chemical biomanufacturing due to its abundance and easy availability. Microbial methylotrophy mainly refers to the ability to utilize reduced one-carbon compounds such as methanol, methane or formaldehyde as sole or primary sources of carbon and energy to sustain growth. Besides native methylotrophic microbes, such as Komagataella phaffii (K phaffii), Methylobacterium extorquens (M. extorquens), Bacillus methanolicus (B. methanolicus), many other yeasts or bacteria have been engineered to exhibit methylotrophy or by reconstructing methanol utilization pathways. This significantly broadens the scenario of methanol metabolism and leverages the well-established synthetic biology toolbox of platform microorganisms like Escherichia coli (E. coli). This article delves into the detailed examination of methanol metabolic pathways (including formaldehyde and formate) and provides an overview of recent progress in metabolic engineering for synthetic methylotrophic microorganisms. It specifically highlights the innovative C1 assimilation pathways employed in artificial methylotrophy. This comprehensive discussion aims to enhance our understanding of methanol metabolism and unlock the industrial potential of synthetic methylotrophs.</p>","PeriodicalId":8946,"journal":{"name":"Biotechnology advances","volume":" ","pages":"108623"},"PeriodicalIF":12.1,"publicationDate":"2025-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144274063","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Engineering microbial carbon metabolism for sustainable resource utilization 工程微生物碳代谢可持续资源利用

IF 12.1 1区工程技术

Biotechnology advances Pub Date : 2025-06-02 DOI: 10.1016/j.biotechadv.2025.108622

Xin Meng , Cong Gao , Guipeng Hu , Xiaomin Li , Liming Liu , Jing Wu

引用次数: 0

Targeted analysis of metagenomes: divide and conquer 宏基因组的目标分析：分而治之。

IF 12.1 1区工程技术

Biotechnology advances Pub Date : 2025-06-02 DOI: 10.1016/j.biotechadv.2025.108619

Marios I. Valmas , Konstantinos Kormas , Dimitrios G. Karpouzas , Konstantinos T. Konstantinidis , Svjetlana Dekić Rozman , Nikolina Udiković-Kolić , Mitja N.P. Remus-Emsermann , Sotirios Vasileiadis

{"title":"Targeted analysis of metagenomes: divide and conquer","authors":"Marios I. Valmas , Konstantinos Kormas , Dimitrios G. Karpouzas , Konstantinos T. Konstantinidis , Svjetlana Dekić Rozman , Nikolina Udiković-Kolić , Mitja N.P. Remus-Emsermann , Sotirios Vasileiadis","doi":"10.1016/j.biotechadv.2025.108619","DOIUrl":"10.1016/j.biotechadv.2025.108619","url":null,"abstract":"<div><div>The rapidly developing field of targeted analysis of metagenomes focuses on retrieving information about specific genes and/or genome(s) from environmental DNA. The traditional shotgun sequencing methods overemphasise dominant microorganisms and often fail to confidently assign the entirety of the analysed genetic material to specific species, genomovars, or strains. The ultimate goal of the targeted methods is to overcome this limitation of throughput and precision of current shotgun metagenomics when analysing complex microbial communities in the quest of refined information. Here, we discuss recent technological advances that are designed to focus the analytical power of diagnostic tools like sequencing, towards phylogenetically or functionally distinct and rare microbial groups and enhance e.g. the confidence in the assignment of genetic elements to their respective owning organisms. We specifically showcase the capabilities of these technological advances for targeted analysis of metagenomes, identify suitable related applications, discuss methodological limitations, and propose solutions for addressing these limitations. This review aspires to inspire highly relevant experimental designs in the future that will unlock unknown and important aspects of microbial ecology, and the yet-uncultivated microbial majority.</div></div>","PeriodicalId":8946,"journal":{"name":"Biotechnology advances","volume":"83 ","pages":"Article 108619"},"PeriodicalIF":12.1,"publicationDate":"2025-06-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144224190","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The road less traveled: Unexplored targets in the quest for antibiotics against Pseudomonas aeruginosa 少有人走过的路：寻找铜绿假单胞菌抗生素的未开发目标

IF 12.1 1区工程技术

Biotechnology advances Pub Date : 2025-06-01 DOI: 10.1016/j.biotechadv.2025.108621

Mohammad Abavisani , Erfan Fazeli , Negar Ebadpour , Sercan Karav , Prashant Kesharwani , Amirhossein Sahebkar

{"title":"The road less traveled: Unexplored targets in the quest for antibiotics against Pseudomonas aeruginosa","authors":"Mohammad Abavisani , Erfan Fazeli , Negar Ebadpour , Sercan Karav , Prashant Kesharwani , Amirhossein Sahebkar","doi":"10.1016/j.biotechadv.2025.108621","DOIUrl":"10.1016/j.biotechadv.2025.108621","url":null,"abstract":"<div><div><em>Pseudomonas aeruginosa</em>, an opportunistic pathogen known for its adaptability, has become a critical health concern due to its inherent resistance to multiple antibiotic classes and its rapid acquisition of new resistance mechanisms. The rise of multidrug-resistant (MDR) and extensively drug-resistant (XDR) strains has further compounded the global burden of <em>P. aeruginosa</em> infections. Traditional antibiotic discovery efforts, which focus on essential bacterial processes such as cell wall synthesis, protein production, and DNA replication, have been unable to keep pace with the pathogen's evolving resistance strategies. Recent advancements in omics technologies have provided deeper insights into the complex biology of <em>P. aeruginosa</em>, including bacterial communication networks like quorum sensing and interactions between the host and pathogen that are crucial for the pathogen's survival and virulence. These insights pave the way for identifying novel therapeutic targets, such as unexplored metabolic pathways and virulence mechanisms, which could offer more effective strategies for combating resistant <em>P. aeruginosa</em> strains. In this review, we critically assess the limitations of conventional approaches and emphasize the potential of targeting these alternative pathways to address the growing challenge of antibiotic resistance. By exploring innovative strategies that transcend traditional methods, this review underscores the importance of pursuing novel therapeutic avenues that could lead to the development of more effective antibiotics against <em>P. aeruginosa</em> and similar resistant pathogens.</div></div>","PeriodicalId":8946,"journal":{"name":"Biotechnology advances","volume":"83 ","pages":"Article 108621"},"PeriodicalIF":12.1,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144203975","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Potential for microbial plastic degradation via assimilation of non-carbon moieties in additives 通过添加剂中非碳部分的同化，微生物降解塑料的潜力。

IF 12.1 1区工程技术

Biotechnology advances Pub Date : 2025-05-30 DOI: 10.1016/j.biotechadv.2025.108620

Dani Wijaya , Japareng Lalung , Syahidah Akmal Muhammad , Peter Convey , Faradina Merican , Fauziah Shahul Hamid , Faisal Allafi

引用次数: 0

Olfactory biosensor for smart agriculture 智能农业嗅觉生物传感器。

IF 12.1 1区工程技术

Biotechnology advances Pub Date : 2025-05-29 DOI: 10.1016/j.biotechadv.2025.108611

Xujun Chen , Longgang Ma , Zhengzhong Wan, Ruihua Zhang, Maoyuan Yin, Zhencan Yang, Xinqing Xiao

引用次数: 0

Advanced therapy medicinal products development - from guidelines to medicines in the market 先进治疗药物产品开发-从指南到市场上的药物

IF 12.1 1区工程技术

Biotechnology advances Pub Date : 2025-05-22 DOI: 10.1016/j.biotechadv.2025.108612

Catarina Frederico , André Conceição , Clévio Nóbrega , Liliana S. Mendonça

{"title":"Advanced therapy medicinal products development - from guidelines to medicines in the market","authors":"Catarina Frederico , André Conceição , Clévio Nóbrega , Liliana S. Mendonça","doi":"10.1016/j.biotechadv.2025.108612","DOIUrl":"10.1016/j.biotechadv.2025.108612","url":null,"abstract":"<div><div>In Europe, Advanced Therapy Medicinal Products (ATMPs) include medicines based on gene therapy, somatic-cell therapy, tissue-engineered products, and combined ATMPs. ATMPs constitute an emerging and innovative class of medicines used to treat multiple pathologies and are particularly relevant in pathologies where therapeutic options are limited and require high medical needs. These therapies act, among others, through the insertion of recombinant nucleic acids, including genes, to promote a therapeutic effect and through the restoration of cell functions, and repairing or replacing damaged cells and tissues impaired in pathological conditions. Despite their unique potential, these therapies face challenges related to scientific complexity, production processes, regulatory approval, and market access that hinder their development and availability.</div><div>Based on official European guidelines, the present review explores the current regulatory framework for the non-clinical and clinical development of advanced therapies. We aimed to discuss the regulations applied to the different types of ATMPs, as well as the challenges associated with their development until these therapies reach the market. Accordingly, topics such as the implementation of proof-of-concept studies to provide evidence supporting the potential clinical effect; biodistribution studies to evaluate tissue distribution and persistence; and toxicology studies to assess potential undesirable effects, integration potential of viral vectors, tumorigenicity, and germline transmission, are discussed. This work also covers some of the ATMPs available to patients on the EU market.</div></div>","PeriodicalId":8946,"journal":{"name":"Biotechnology advances","volume":"83 ","pages":"Article 108612"},"PeriodicalIF":12.1,"publicationDate":"2025-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144139114","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

CRISPR/Cas-powered nucleic acid amplification and amplification-free biosensors for public safety detection: Principles, advances and prospects 用于公共安全检测的CRISPR/ cas驱动的核酸扩增和无扩增生物传感器：原理、进展与展望

IF 12.1 1区工程技术

Biotechnology advances Pub Date : 2025-05-21 DOI: 10.1016/j.biotechadv.2025.108609

Bo Yi , Baoqing Zhou , Donggen Zhou , Luyu Yang , Hengyi Xu

{"title":"CRISPR/Cas-powered nucleic acid amplification and amplification-free biosensors for public safety detection: Principles, advances and prospects","authors":"Bo Yi , Baoqing Zhou , Donggen Zhou , Luyu Yang , Hengyi Xu","doi":"10.1016/j.biotechadv.2025.108609","DOIUrl":"10.1016/j.biotechadv.2025.108609","url":null,"abstract":"<div><div>Rapid, accurate, cost-effective, and efficient ultrasensitive detection strategies are essential for public health safety (including food safety, disease prevention and environmental governance). The CRISPR/CRISPR-associated (Cas) detection is a cutting-edge technology that has been widely used in the detection of public health safety due to its targeted cleavage properties (signal amplification), attomolar level sensitivity, high specificity (recognizing single-base mismatches), and rapid turnover time. However, the current research about CRISPR/Cas-based biosensors is not clear, such as mechanism problem and application differences of integrating CRISPR/Cas system with other technologies, and how to further innovate and develop in the future. Therefore, further detailed analysis and comparative discussion of CRISPR/Cas-based biosensors is needed. Currently, CRISPR/Cas system powered biosensors can be mainly categorized into two types: CRISPR/Cas system powered nucleic acid amplification biosensors and CRISPR/Cas system powered nucleic acid amplification-free biosensors. The two biosensors have different characteristics and advantages. This paper first provides an in-depth investigation of the enzymatic mechanism of CRISPR/Cas system at the molecular level. Then, this paper summarizes the principles and recent advances of CRISPR/Cas system powered nucleic acid amplification biosensors and CRISPR/Cas system powered nucleic acid amplification-free biosensors and discusses their integration mechanisms in depth. More, the differences and application-oriented between the two biosensors are further discussed. Finally, the application orientation and future perspectives of the two biosensors are discussed, and unique insights into the future development of CRISPR/Cas system are provided.</div></div>","PeriodicalId":8946,"journal":{"name":"Biotechnology advances","volume":"83 ","pages":"Article 108609"},"PeriodicalIF":12.1,"publicationDate":"2025-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144131903","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0