Biotechnology advances最新文献_第10页

Engineering conditional protein-protein interactions for dynamic cellular control 设计条件性蛋白质-蛋白质相互作用，实现动态细胞控制。

IF 12.1 1区工程技术

Biotechnology advances Pub Date : 2024-09-27 DOI: 10.1016/j.biotechadv.2024.108457

Anthony M. Stohr, Derron Ma, Wilfred Chen, Mark Blenner

引用次数: 0

Microbiome regulation for sustainable wastewater treatment 可持续废水处理的微生物组调控。

IF 12.1 1区工程技术

Biotechnology advances Pub Date : 2024-09-27 DOI: 10.1016/j.biotechadv.2024.108458

Ke Shi , Jia-Min Xu , Han-Lin Cui, Hao-Yi Cheng, Bin Liang, Ai-Jie Wang

{"title":"Microbiome regulation for sustainable wastewater treatment","authors":"Ke Shi , Jia-Min Xu , Han-Lin Cui, Hao-Yi Cheng, Bin Liang, Ai-Jie Wang","doi":"10.1016/j.biotechadv.2024.108458","DOIUrl":"10.1016/j.biotechadv.2024.108458","url":null,"abstract":"<div><div>Sustainable wastewater treatment is essential for attaining clean water and sanitation, aligning with UN Sustainable Development Goals. Wastewater treatment plants (WWTPs) have utilized environmental microbiomes in biological treatment processes in this effort for over a century. However, the inherent complexity and redundancy of microbial communities, and emerging chemical and biological contaminants, challenge the biotechnology applications. Over the past decades, understanding and utilization of microbial energy metabolism and interaction relationships have revolutionized the biological system. In this review, we discuss how microbiome regulation strategies are being used to generate actionable performance for low-carbon pollutant removal and resource recovery in WWTPs. The engineering application cases also highlight the real feasibility and promising prospects of the microbiome regulation approaches. In conclusion, we recommend identifying environmental risks associated with chemical and biological contaminants transformation as a prerequisite. We propose the integration of gene editing and enzyme design to precisely regulate microbiomes for the synergistic control of both chemical and biological risks. Additionally, the development of integrated technologies and engineering equipment is crucial in addressing the ongoing water crisis. This review advocates for the innovation of conventional wastewater treatment biotechnology to ensure sustainable wastewater treatment.</div></div>","PeriodicalId":8946,"journal":{"name":"Biotechnology advances","volume":"77 ","pages":"Article 108458"},"PeriodicalIF":12.1,"publicationDate":"2024-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142340990","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bioengineered heparin: Advances in production technology 生物工程肝素：生产技术的进步。

IF 12.1 1区工程技术

Biotechnology advances Pub Date : 2024-09-24 DOI: 10.1016/j.biotechadv.2024.108456

Razia Sultana , Masamichi Kamihira

{"title":"Bioengineered heparin: Advances in production technology","authors":"Razia Sultana , Masamichi Kamihira","doi":"10.1016/j.biotechadv.2024.108456","DOIUrl":"10.1016/j.biotechadv.2024.108456","url":null,"abstract":"<div><div>Heparin, a highly sulfated glycosaminoglycan, is considered an indispensable anticoagulant with diverse therapeutic applications and has been a mainstay in medical practice for nearly a century. Its potential extends beyond anticoagulation, showing promise in treating inflammation, cancer, and infectious diseases such as COVID-19. However, its current sourcing from animal tissues poses challenges due to variable structures and adulterations, impacting treatment efficacy and safety. Recent advancements in metabolic engineering and synthetic biology offer alternatives through bioengineered heparin production, albeit with challenges such as controlling molecular weight and sulfonation patterns. This review offers comprehensive insight into recent advancements, encompassing: (i) the metabolic engineering strategies in prokaryotic systems for heparin production; (ii) strides made in the development of bioengineered heparin; and (iii) groundbreaking approaches driving production enhancements in eukaryotic systems. Additionally, it explores the potential of recombinant Chinese hamster ovary cells in heparin synthesis, discussing recent progress, challenges, and future prospects, thereby opening up new avenues in biomedical research.</div></div>","PeriodicalId":8946,"journal":{"name":"Biotechnology advances","volume":"77 ","pages":"Article 108456"},"PeriodicalIF":12.1,"publicationDate":"2024-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142340988","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Advances in biosynthesis and downstream processing of diols 二元醇的生物合成和下游加工取得进展。

IF 12.1 1区工程技术

Biotechnology advances Pub Date : 2024-09-19 DOI: 10.1016/j.biotechadv.2024.108455

Yongfei Liu , Chijian Zhang , An-Ping Zeng

引用次数: 0

Advances in multi-enzyme co-localization strategies for the construction of microbial cell factory 构建微生物细胞工厂的多酶共定位策略的进展

IF 12.1 1区工程技术

Biotechnology advances Pub Date : 2024-09-13 DOI: 10.1016/j.biotechadv.2024.108453

Qi Guo , Yu-Xin Yang , Dong-Xun Li , Xiao-Jun Ji , Na Wu , Yue-Tong Wang , Chao Ye , Tian-Qiong Shi

{"title":"Advances in multi-enzyme co-localization strategies for the construction of microbial cell factory","authors":"Qi Guo , Yu-Xin Yang , Dong-Xun Li , Xiao-Jun Ji , Na Wu , Yue-Tong Wang , Chao Ye , Tian-Qiong Shi","doi":"10.1016/j.biotechadv.2024.108453","DOIUrl":"10.1016/j.biotechadv.2024.108453","url":null,"abstract":"<div><p>Biomanufacturing, driven by technologies such as synthetic biology, offers significant potential to advance the bioeconomy and promote sustainable development. It is anticipated to transform traditional manufacturing and become a key industry in future strategies. Cell factories are the core of biomanufacturing. The advancement of synthetic biology and growing market demand have led to the production of a greater variety of natural products and increasingly complex metabolic pathways. However, this progress also presents challenges, notably the conflict between natural product production and chassis cell growth. This conflict results in low productivity and yield, adverse side effects, metabolic imbalances, and growth retardation. Enzyme co-localization strategies have emerged as a promising solution. This article reviews recent progress and applications of these strategies in constructing cell factories for efficient natural product production. It comprehensively describes the applications of enzyme-based compartmentalization, metabolic pathway-based compartmentalization, and synthetic organelle-based compartmentalization in improving product titers. The article also explores future research directions and the prospects of combining multiple strategies with advanced technologies.</p></div>","PeriodicalId":8946,"journal":{"name":"Biotechnology advances","volume":"77 ","pages":"Article 108453"},"PeriodicalIF":12.1,"publicationDate":"2024-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142243033","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The biosynthesis of L-phenylalanine-derived compounds by engineered microbes 工程微生物对 L-苯丙氨酸衍生化合物的生物合成

IF 12.1 1区工程技术

Biotechnology advances Pub Date : 2024-09-11 DOI: 10.1016/j.biotechadv.2024.108448

Min Qiu , Jie Jiang , Wankui Jiang , Wenming Zhang , Yujia Jiang , Fengxue Xin , Min Jiang

{"title":"The biosynthesis of L-phenylalanine-derived compounds by engineered microbes","authors":"Min Qiu , Jie Jiang , Wankui Jiang , Wenming Zhang , Yujia Jiang , Fengxue Xin , Min Jiang","doi":"10.1016/j.biotechadv.2024.108448","DOIUrl":"10.1016/j.biotechadv.2024.108448","url":null,"abstract":"<div><p>L-Phenylalanine (L-Phe) is an important aromatic amino acid, which has been widely used in food, health care products, medicine and other fields. Based on the relatively mature microbial biosynthesis process, a variety of <em>L</em>-phenylalanine-derived compounds have attracted more and more attentions owing to their extensively potential applications in the fields of food, medicine, spices, cosmetics, and pesticides. However, the challenge of biosynthesis of <em>L</em>-phenylalanine-derived compounds remains the issue of low production and productivity. With the development of metabolic engineering and synthetic biology, the biosynthesis of <em>L</em>-phenylalanine has reached a high level. Therefore, the synthesis of <em>L</em>-phenylalanine-derived compounds based on high production strains of <em>L</em>-phenylalanine has broad prospects. In addition, some <em>L</em>-phenylalanine-derived compounds are more suitable for efficient synthesis by exogenous addition of precursors due to their longer metabolic pathways and the inhibitory effects of many intermediate products. This review systematically summarized the research progress of <em>L</em>-phenylalanine-derived compounds, including phenylpyruvate derivatives, <em>trans</em>-cinnamic derivatives, <em>p</em>-coumaric acid derivatives and other <em>L</em>-phenylalanine-derived compounds (such as flavonoids). Finally, the main strategies to improve the production of <em>L</em>-phenylalanine-derived compounds were summarized, and the development trends of the synthesis of <em>L</em>-phenylalanine-derived compounds by microbial method were also prospected.</p></div>","PeriodicalId":8946,"journal":{"name":"Biotechnology advances","volume":"77 ","pages":"Article 108448"},"PeriodicalIF":12.1,"publicationDate":"2024-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142167376","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Droplet-based single-cell sequencing: Strategies and applications 基于液滴的单细胞测序：策略与应用

IF 12.1 1区工程技术

Biotechnology advances Pub Date : 2024-09-11 DOI: 10.1016/j.biotechadv.2024.108454

Yuting Shang , Zhengzheng Wang , Liqing Xi , Yantao Wang , Meijing Liu , Ying Feng , Juan Wang , Qingping Wu , Xinran Xiang , Moutong Chen , Yu Ding

{"title":"Droplet-based single-cell sequencing: Strategies and applications","authors":"Yuting Shang , Zhengzheng Wang , Liqing Xi , Yantao Wang , Meijing Liu , Ying Feng , Juan Wang , Qingping Wu , Xinran Xiang , Moutong Chen , Yu Ding","doi":"10.1016/j.biotechadv.2024.108454","DOIUrl":"10.1016/j.biotechadv.2024.108454","url":null,"abstract":"<div><p>Notable advancements in single-cell omics technologies have not only addressed longstanding challenges but also enabled unprecedented studies of cellular heterogeneity with unprecedented resolution and scale. These strides have led to groundbreaking insights into complex biological systems, paving the way for a more profound comprehension of human biology and diseases. The droplet microfluidic technology has become a crucial component in many single-cell sequencing workflows in terms of throughput, cost-effectiveness, and automation. Utilizing a microfluidic chip to encapsulate and profile individual cells within droplets has significantly improved single-cell research. Therefore, this review aims to comprehensively elaborate the droplet microfluidics-assisted omics methods from a single-cell perspective. The strategies for using droplet microfluidics in the realms of genomics, epigenomics, transcriptomics, and proteomics analyses are first introduced. On this basis, the focus then turns to the latest applications of this technology in different sequencing patterns, including mono- and multi-omics. Finally, the challenges and further perspectives of droplet-based single-cell sequencing in both foundational research and commercial applications are discussed.</p></div>","PeriodicalId":8946,"journal":{"name":"Biotechnology advances","volume":"77 ","pages":"Article 108454"},"PeriodicalIF":12.1,"publicationDate":"2024-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142242996","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Recent advances in engineering synthetic biomolecular condensates 合成生物分子凝聚态工程的最新进展

IF 12.1 1区工程技术

Biotechnology advances Pub Date : 2024-09-11 DOI: 10.1016/j.biotechadv.2024.108452

Li Wan, Juntao Ke, Yingying Zhu, Wenli Zhang, Wanmeng Mu

{"title":"Recent advances in engineering synthetic biomolecular condensates","authors":"Li Wan, Juntao Ke, Yingying Zhu, Wenli Zhang, Wanmeng Mu","doi":"10.1016/j.biotechadv.2024.108452","DOIUrl":"10.1016/j.biotechadv.2024.108452","url":null,"abstract":"<div><p>Biomolecular condensates are intriguing entities found within living cells. These structures possess the ability to selectively concentrate specific components through phase separation, thereby playing a crucial role in the spatiotemporal regulation of a wide range of cellular processes and metabolic activities. To date, extensive studies have been dedicated to unraveling the intricate connections between molecular features, physical properties, and cellular functions of condensates. This collective effort has paved the way for deliberate engineering of tailor-made condensates with specific applications. In this review, we comprehensively examine the underpinnings governing condensate formation. Next, we summarize the material states of condensates and delve into the design of synthetic intrinsically disordered proteins with tunable phase behaviors and physical properties. Subsequently, we review the diverse biological functions demonstrated by synthetic biomolecular condensates, encompassing gene regulation, cellular behaviors, modulation of biochemical reactions, and manipulation of endogenous protein activities. Lastly, we discuss future challenges and opportunities in constructing synthetic condensates with tunable physical properties and customized cellular functions, which may shed light on the development of new types of sophisticated condensate systems with distinct functions applicable to various scenarios.</p></div>","PeriodicalId":8946,"journal":{"name":"Biotechnology advances","volume":"77 ","pages":"Article 108452"},"PeriodicalIF":12.1,"publicationDate":"2024-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142228987","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Production of marine-derived bioactive peptide molecules for industrial applications: A reverse engineering approach 为工业应用生产海洋生物活性肽分子：逆向工程方法

IF 12.1 1区工程技术

Biotechnology advances Pub Date : 2024-09-10 DOI: 10.1016/j.biotechadv.2024.108449

Akash J. Surwase , Narsinh L. Thakur

引用次数: 0

Advance computational tools for multiomics data learning 用于多组学数据学习的先进计算工具

IF 12.1 1区工程技术

Biotechnology advances Pub Date : 2024-09-07 DOI: 10.1016/j.biotechadv.2024.108447

Sheikh Mansoor , Saira Hamid , Thai Thanh Tuan , Jong-Eun Park , Yong Suk Chung

引用次数: 0