Biotechnology advances最新文献_第10页

Recent advances in engineering synthetic biomolecular condensates 合成生物分子凝聚态工程的最新进展

IF 12.1 1区工程技术

Biotechnology advances Pub Date : 2024-09-11 DOI: 10.1016/j.biotechadv.2024.108452

Li Wan, Juntao Ke, Yingying Zhu, Wenli Zhang, Wanmeng Mu

{"title":"Recent advances in engineering synthetic biomolecular condensates","authors":"Li Wan, Juntao Ke, Yingying Zhu, Wenli Zhang, Wanmeng Mu","doi":"10.1016/j.biotechadv.2024.108452","DOIUrl":"10.1016/j.biotechadv.2024.108452","url":null,"abstract":"<div><p>Biomolecular condensates are intriguing entities found within living cells. These structures possess the ability to selectively concentrate specific components through phase separation, thereby playing a crucial role in the spatiotemporal regulation of a wide range of cellular processes and metabolic activities. To date, extensive studies have been dedicated to unraveling the intricate connections between molecular features, physical properties, and cellular functions of condensates. This collective effort has paved the way for deliberate engineering of tailor-made condensates with specific applications. In this review, we comprehensively examine the underpinnings governing condensate formation. Next, we summarize the material states of condensates and delve into the design of synthetic intrinsically disordered proteins with tunable phase behaviors and physical properties. Subsequently, we review the diverse biological functions demonstrated by synthetic biomolecular condensates, encompassing gene regulation, cellular behaviors, modulation of biochemical reactions, and manipulation of endogenous protein activities. Lastly, we discuss future challenges and opportunities in constructing synthetic condensates with tunable physical properties and customized cellular functions, which may shed light on the development of new types of sophisticated condensate systems with distinct functions applicable to various scenarios.</p></div>","PeriodicalId":8946,"journal":{"name":"Biotechnology advances","volume":"77 ","pages":"Article 108452"},"PeriodicalIF":12.1,"publicationDate":"2024-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142228987","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Production of marine-derived bioactive peptide molecules for industrial applications: A reverse engineering approach 为工业应用生产海洋生物活性肽分子：逆向工程方法

IF 12.1 1区工程技术

Biotechnology advances Pub Date : 2024-09-10 DOI: 10.1016/j.biotechadv.2024.108449

Akash J. Surwase , Narsinh L. Thakur

引用次数: 0

Advance computational tools for multiomics data learning 用于多组学数据学习的先进计算工具

IF 12.1 1区工程技术

Biotechnology advances Pub Date : 2024-09-07 DOI: 10.1016/j.biotechadv.2024.108447

Sheikh Mansoor , Saira Hamid , Thai Thanh Tuan , Jong-Eun Park , Yong Suk Chung

引用次数: 0

From natural to synthetic: Promoter engineering in yeast expression systems 从天然到合成：酵母表达系统中的启动子工程。

IF 12.1 1区工程技术

Biotechnology advances Pub Date : 2024-09-07 DOI: 10.1016/j.biotechadv.2024.108446

Ly Nguyen , Bernhard Schmelzer , Stuart Wilkinson , Diethard Mattanovich

{"title":"From natural to synthetic: Promoter engineering in yeast expression systems","authors":"Ly Nguyen , Bernhard Schmelzer , Stuart Wilkinson , Diethard Mattanovich","doi":"10.1016/j.biotechadv.2024.108446","DOIUrl":"10.1016/j.biotechadv.2024.108446","url":null,"abstract":"<div><p>Synthetic promoters are particularly relevant for application not only in yeast expression systems designed for high-level heterologous protein production but also in other applications such as metabolic engineering, cell biological research, and stage-specific gene expression control. By designing synthetic promoters, researcher can create customized expression systems tailored to specific needs, whether it is maximizing protein production or precisely controlling gene expression at different stages of a process. While recognizing the limitations of endogenous promoters, they also provide important information needed to design synthetic promoters. In this review, emphasis will be placed on some key approaches to identify endogenous, and to generate synthetic promoters in yeast expression systems. It shows the connection between endogenous and synthetic promoters, highlighting how their interplay contributes to promoter development. Furthermore, this review illustrates recent developments in biotechnological advancements and discusses how this field will evolve in order to develop custom-made promoters for diverse applications. This review offers detailed information, explores the transition from endogenous to synthetic promoters, and presents valuable perspectives on the next generation of promoter design strategies.</p></div>","PeriodicalId":8946,"journal":{"name":"Biotechnology advances","volume":"77 ","pages":"Article 108446"},"PeriodicalIF":12.1,"publicationDate":"2024-09-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S073497502400140X/pdfft?md5=81914edd11ad9cc6e44d7ac302f9a97f&pid=1-s2.0-S073497502400140X-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142153113","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An overview on polyurethane-degrading enzymes 聚氨酯降解酶概述。

IF 12.1 1区工程技术

Biotechnology advances Pub Date : 2024-09-04 DOI: 10.1016/j.biotechadv.2024.108439

Agata Raczyńska , Artur Góra , Isabelle André

{"title":"An overview on polyurethane-degrading enzymes","authors":"Agata Raczyńska , Artur Góra , Isabelle André","doi":"10.1016/j.biotechadv.2024.108439","DOIUrl":"10.1016/j.biotechadv.2024.108439","url":null,"abstract":"<div><p>Polyurethanes (PUR) are durable synthetic polymers widely used in various industries, contributing significantly to global plastic consumption. PUR pose unique challenges in terms of degradability and recyclability, as they are characterised by intricate compositions and diverse formulations. Additives and proprietary structures used in commercial PUR formulations further complicate recycling efforts, making the effective management of PUR waste a daunting task.</p><p>In this review, we delve into the complex challenge of enzymatic degradation of PUR, focusing on the structural and functional attributes of both enzymes and PUR. We also present documented native enzymes with reported efficacy in hydrolysing specific bonds within PUR, analysis of these enzyme structures, reaction mechanisms, substrate specificity, and binding site architecture. Furthermore, we propose essential features for the future redesign of enzymes to optimise PUR biodegradation efficiency. By outlining prospective research directions aimed at advancing the field of enzymatic biodegradation of PUR, we aim to contribute to the development of sustainable solutions for managing PUR waste and reducing environmental pollution.</p></div>","PeriodicalId":8946,"journal":{"name":"Biotechnology advances","volume":"77 ","pages":"Article 108439"},"PeriodicalIF":12.1,"publicationDate":"2024-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142145036","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Pathway to industrial application of heterotrophic organisms in critical metals recycling from e-waste 异养生物在电子垃圾关键金属回收中的工业应用途径。

IF 12.1 1区工程技术

Biotechnology advances Pub Date : 2024-08-30 DOI: 10.1016/j.biotechadv.2024.108438

Mehdi Golzar-Ahmadi , Nazanin Bahaloo-Horeh , Fatemeh Pourhossein , Forough Norouzi , Nora Schoenberger , Christian Hintersatz , Mital Chakankar , Maria Holuszko , Anna H. Kaksonen

{"title":"Pathway to industrial application of heterotrophic organisms in critical metals recycling from e-waste","authors":"Mehdi Golzar-Ahmadi , Nazanin Bahaloo-Horeh , Fatemeh Pourhossein , Forough Norouzi , Nora Schoenberger , Christian Hintersatz , Mital Chakankar , Maria Holuszko , Anna H. Kaksonen","doi":"10.1016/j.biotechadv.2024.108438","DOIUrl":"10.1016/j.biotechadv.2024.108438","url":null,"abstract":"<div><p>The transition to renewable energies and electric vehicles has triggered an unprecedented demand for metals. Sustainable development of these technologies relies on effectively managing the lifecycle of critical raw materials, including their responsible sourcing, efficient use, and recycling. Metal recycling from electronic waste (e-waste) is of paramount importance owing to ore-exceeding amounts of critical elements and high toxicity of heavy metals and organic pollutants in e-waste to the natural ecosystem and human body. Heterotrophic microbes secrete numerous metal-binding biomolecules such as organic acids, amino acids, cyanide, siderophores, peptides, and biosurfactants which can be utilized for eco-friendly and profitable metal recycling. In this review paper, we presented a critical review of heterotrophic organisms in biomining, and current barriers hampering the industrial application of organic acid bioleaching and biocyanide leaching. We also discussed how these challenges can be surmounted with simple methods (e.g., culture media optimization, separation of microbial growth and metal extraction process) and state-of-the-art biological approaches (e.g., artificial microbial community, synthetic biology, metabolic engineering, advanced fermentation strategies, and biofilm engineering). Lastly, we showcased emerging technologies (e.g., artificially synthesized peptides, siderophores, and biosurfactants) derived from heterotrophs with the potential for inexpensive, low-impact, selective and advanced metal recovery from bioleaching solutions.</p></div>","PeriodicalId":8946,"journal":{"name":"Biotechnology advances","volume":"77 ","pages":"Article 108438"},"PeriodicalIF":12.1,"publicationDate":"2024-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0734975024001320/pdfft?md5=2a7edaf45f27d2d6647833907e78532e&pid=1-s2.0-S0734975024001320-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142104016","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Computational strategies in Klebsiella pneumoniae vaccine design: navigating the landscape of in silico insights. 肺炎克雷伯氏菌疫苗设计中的计算策略：浏览硅学洞察的全貌。

IF 12.1 1区工程技术

Biotechnology advances Pub Date : 2024-08-30 DOI: 10.1016/j.biotechadv.2024.108437

Bruno Douradinha

{"title":"Computational strategies in Klebsiella pneumoniae vaccine design: navigating the landscape of in silico insights.","authors":"Bruno Douradinha","doi":"10.1016/j.biotechadv.2024.108437","DOIUrl":"10.1016/j.biotechadv.2024.108437","url":null,"abstract":"<div><p>The emergence of multidrug-resistant <em>Klebsiella pneumoniae</em> poses a grave threat to global public health, necessitating urgent strategies for vaccine development. In this context, computational tools have emerged as indispensable assets, offering unprecedented insights into klebsiellal biology and facilitating the design of effective vaccines. Here, a review of the application of computational methods in the development of <em>K. pneumoniae</em> vaccines is presented, elucidating the transformative impact of in silico approaches. Through a systematic exploration of bioinformatics, structural biology, and immunoinformatics techniques, the complex landscape of <em>K. pneumoniae</em> pathogenesis and antigenicity was unravelled. Key insights into virulence factors, antigen discovery, and immune response mechanisms are discussed, highlighting the pivotal role of computational tools in accelerating vaccine development efforts. Advancements in epitope prediction, antigen selection, and vaccine design optimisation are examined, highlighting the potential of in silico approaches to update vaccine development pipelines. Furthermore, challenges and future directions in leveraging computational tools to combat <em>K. pneumoniae</em> are discussed, emphasizing the importance of multidisciplinary collaboration and data integration. This review provides a comprehensive overview of the current state of computational contributions to <em>K. pneumoniae</em> vaccine development, offering insights into innovative strategies for addressing this urgent global health challenge.</p></div>","PeriodicalId":8946,"journal":{"name":"Biotechnology advances","volume":"76 ","pages":"Article 108437"},"PeriodicalIF":12.1,"publicationDate":"2024-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142095178","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biotechnological advances in the production of unusual fatty acids in transgenic plants and recombinant microorganisms 转基因植物和重组微生物在生产特殊脂肪酸方面的生物技术进步

IF 12.1 1区工程技术

Biotechnology advances Pub Date : 2024-08-28 DOI: 10.1016/j.biotechadv.2024.108435

Juli Wang , Stacy D. Singer , Guanqun Chen

{"title":"Biotechnological advances in the production of unusual fatty acids in transgenic plants and recombinant microorganisms","authors":"Juli Wang , Stacy D. Singer , Guanqun Chen","doi":"10.1016/j.biotechadv.2024.108435","DOIUrl":"10.1016/j.biotechadv.2024.108435","url":null,"abstract":"<div><p>Certain plants and microorganisms can produce high amounts of unusual fatty acids (UFAs) such as hydroxy, conjugated, cyclic, and very long-chain polyunsaturated fatty acids, which have distinct physicochemical properties and significant applications in the food, feed, and oleochemical industries. Since many natural sources of UFAs are not ideal for large-scale agricultural production or fermentation, it is attractive to produce them through synthetic biology. Although several UFAs have been commercially or pre-commercially produced in transgenic plants and microorganisms, their contents in transgenic hosts are generally much lower than in natural sources. Moreover, reproducing this success for a wider spectrum of UFAs has remained challenging. This review discusses recent advancements in our understanding of the biosynthesis, accumulation, and heterologous production of UFAs, and addresses the challenges and potential strategies for achieving high UFA content in engineered plants and microorganisms.</p></div>","PeriodicalId":8946,"journal":{"name":"Biotechnology advances","volume":"76 ","pages":"Article 108435"},"PeriodicalIF":12.1,"publicationDate":"2024-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0734975024001290/pdfft?md5=d382e77cfb86a2151f88191de72ef94b&pid=1-s2.0-S0734975024001290-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142095355","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Recent advances in the construction strategy, functional properties, and biosensing application of self-assembled triangular unit-based DNA nanostructures 基于自组装三角形单元的 DNA 纳米结构的构建策略、功能特性和生物传感应用的最新进展。

IF 12.1 1区工程技术

Biotechnology advances Pub Date : 2024-08-28 DOI: 10.1016/j.biotechadv.2024.108436

Mengxia Duan , Yuting Chang , Xiaowan Chen , Zhouping Wang , Shijia Wu , Nuo Duan

{"title":"Recent advances in the construction strategy, functional properties, and biosensing application of self-assembled triangular unit-based DNA nanostructures","authors":"Mengxia Duan , Yuting Chang , Xiaowan Chen , Zhouping Wang , Shijia Wu , Nuo Duan","doi":"10.1016/j.biotechadv.2024.108436","DOIUrl":"10.1016/j.biotechadv.2024.108436","url":null,"abstract":"<div><p>Research on self-assembled deoxyribonucleic acid (DNA) nanostructures with different shapes, sizes, and functions has recently made rapid progress owing to its biocompatibility, programmability, and stability. Among these, triangular unit-based DNA nanostructures, which are typically multi-arm DNA tiles, have been widely applied because of their unique structural rigidity, spatial flexibility, and cell permeability. Triangular unit-based DNA nanostructures are folded from multiple single-stranded DNA using the principle of complementary base pairing. Its shape and size can be determined using pre-set scaffold strands, segmented base complementary regions, and sequence lengths. The resulting DNA nanostructures retain the desired sequence length to serve as binding sites for other molecules and obtain satisfactory results in molecular recognition, spatial orientation, and target acquisition. Therefore, extensive research on triangular unit-based DNA nanostructures has shown that they can be used as powerful tools in the biosensing field to improve specificity, sensitivity, and accuracy. Over the past few decades, various design strategies and assembly techniques have been established to improve the stability, complexity, functionality, and practical applications of triangular unit-based DNA nanostructures in biosensing. In this review, we introduce the structural design strategies and principles of typical triangular unit-based DNA nanostructures, including triangular, tetrahedral, star, and net-shaped DNA. We then summarize the functional properties of triangular unit-based DNA nanostructures and their applications in biosensing. Finally, we critically discuss the existing challenges and future trends.</p></div>","PeriodicalId":8946,"journal":{"name":"Biotechnology advances","volume":"76 ","pages":"Article 108436"},"PeriodicalIF":12.1,"publicationDate":"2024-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142104017","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ex vivo T cell differentiation in adoptive immunotherapy manufacturing: Critical process parameters and analytical technologies 采用性免疫疗法生产中的体外 T 细胞分化：关键工艺参数和分析技术。

IF 12.1 1区工程技术

Biotechnology advances Pub Date : 2024-08-20 DOI: 10.1016/j.biotechadv.2024.108434

Sixun Chen , Tan Dai Nguyen , Kang-Zheng Lee, Dan Liu

{"title":"Ex vivo T cell differentiation in adoptive immunotherapy manufacturing: Critical process parameters and analytical technologies","authors":"Sixun Chen , Tan Dai Nguyen , Kang-Zheng Lee, Dan Liu","doi":"10.1016/j.biotechadv.2024.108434","DOIUrl":"10.1016/j.biotechadv.2024.108434","url":null,"abstract":"<div><p>Adoptive immunotherapy shows great promise as a treatment for cancer and other diseases. Recent evidence suggests that the therapeutic efficacy of these cell-based therapies can be enhanced by the enrichment of less-differentiated T cell subpopulations in the therapeutic product, giving rise to a need for advanced manufacturing technologies capable of enriching these subpopulations through regulation of T cell differentiation. Studies have shown that modifying certain critical process control parameters, such as cytokines, metabolites, amino acids, and culture environment, can effectively manipulate T cell differentiation in <em>ex vivo</em> cultures. Advanced process analytical technologies (PATs) are crucial for monitoring these parameters and the assessment of T cell differentiation during culture. In this review, we examine such critical process parameters and PATs, with an emphasis on their impact on enriching less-differentiated T cell population. We also discuss the limitations of current technologies and advocate for further efforts from the community to establish more stringent critical process parameters (CPPs) and develop more at-line/online PATs that are specific to T cell differentiation. These advancements will be essential to enable the manufacturing of more efficacious adoptive immunotherapy products.</p></div>","PeriodicalId":8946,"journal":{"name":"Biotechnology advances","volume":"77 ","pages":"Article 108434"},"PeriodicalIF":12.1,"publicationDate":"2024-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0734975024001289/pdfft?md5=cbd00ab2ac6a45fc7da67c77ecea09de&pid=1-s2.0-S0734975024001289-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142016284","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0