Biotechnology advances最新文献

{"title":"Development and applications of lipid hydrophilic headgroups for nucleic acid therapy","authors":"Wanting Ma ,&nbsp;Xingxing Fu ,&nbsp;Tianyi Zhao ,&nbsp;Yanfei Qi ,&nbsp;Shubiao Zhang ,&nbsp;Yinan Zhao","doi":"10.1016/j.biotechadv.2024.108395","DOIUrl":"10.1016/j.biotechadv.2024.108395","url":null,"abstract":"<div><p>Nucleic acid therapy is currently the most promising method for treating tumors and genetic diseases and for preventing infectious diseases. However, the biggest obstacle to this therapy is delivery of the nucleic acids to the target site, which requires overcoming problems such as capture by the immune system, the need to penetrate biofilms, and degradation of nucleic acid performance. Designing suitable delivery vectors is key to solving these problems. Lipids—which consist of a hydrophilic headgroup, a linker, and a hydrophobic tail—are crucial components for the construction of vectors. The headgroup is particularly important because it affects the drug encapsulation rate, the vector cytotoxicity, and the transfection efficiency. Herein, we focus on various headgroup structures (tertiary amines, quaternary ammonium salts, peptides, piperazines, dendrimers, and several others), and we summarize and classify important lipid-based carriers that have been developed in recent years. We also discuss applications of cationic lipids with various headgroups for delivery of nucleic acid drugs, and we analyze how headgroup structure affects transport efficiency and carrier toxicity. Finally, we briefly describe the challenges of developing novel lipid carriers, as well as their prospects.</p></div>","PeriodicalId":8946,"journal":{"name":"Biotechnology advances","volume":"74 ","pages":"Article 108395"},"PeriodicalIF":12.1,"publicationDate":"2024-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141436625","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enzyme-mediated green synthesis of glycosaminoglycans and catalytic process intensification","authors":"Jie Zheng ,&nbsp;Xiao-jun Lin ,&nbsp;Han Xu ,&nbsp;Muhammad Sohail ,&nbsp;Liang-an Chen ,&nbsp;Xing Zhang","doi":"10.1016/j.biotechadv.2024.108394","DOIUrl":"10.1016/j.biotechadv.2024.108394","url":null,"abstract":"<div><p>Glycosaminoglycans (GAGs) are a family of structurally complex heteropolysaccharides that play pivotal roles in biological functions, including the regulation of cell proliferation, enzyme inhibition, and activation of growth factor receptors. Therefore, the synthesis of GAGs is a hot research topic in drug development. The enzymatic synthesis of GAGs has received widespread attention due to their eco-friendly nature, high regioselectivity, and stereoselectivity. The enhancement of the enzymatic synthesis process is the key to its industrial applications. In this review, we overviewed the construction of more efficient <em>in vitro</em> biomimetic synthesis systems of glycosaminoglycans and presented the different strategies to improve enzyme catalysis, including the combination of chemical and enzymatic methods, solid-phase synthesis, and protein engineering to solve the problems of enzyme stability, separation and purification of the product, preparation of structurally defined sugar chains, <em>etc.</em>, and discussed the challenges and opportunities in large-scale green synthesis of GAGs.</p></div>","PeriodicalId":8946,"journal":{"name":"Biotechnology advances","volume":"74 ","pages":"Article 108394"},"PeriodicalIF":16.0,"publicationDate":"2024-06-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141299954","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Advances and opportunities in process analytical technologies for viral vector manufacturing","authors":"Sobhana A. Sripada ,&nbsp;Mahshid Hosseini ,&nbsp;Srivatsan Ramesh ,&nbsp;Junhyeong Wang ,&nbsp;Kimberly Ritola ,&nbsp;Stefano Menegatti ,&nbsp;Michael A. Daniele","doi":"10.1016/j.biotechadv.2024.108391","DOIUrl":"10.1016/j.biotechadv.2024.108391","url":null,"abstract":"<div><p>Viral vectors are an emerging, exciting class of biologics whose application in vaccines, oncology, and gene therapy has grown exponentially in recent years. Following first regulatory approval, this class of therapeutics has been vigorously pursued to treat monogenic disorders including orphan diseases, entering hundreds of new products into pipelines. Viral vector manufacturing supporting clinical efforts has spurred the introduction of a broad swath of analytical techniques dedicated to assessing the diverse and evolving panel of Critical Quality Attributes (CQAs) of these products. Herein, we provide an overview of the current state of analytics enabling measurement of CQAs such as capsid and vector identities, product titer, transduction efficiency, impurity clearance etc. We highlight orthogonal methods and discuss the advantages and limitations of these techniques while evaluating their adaptation as process analytical technologies. Finally, we identify gaps and propose opportunities in enabling existing technologies for real-time monitoring from hardware, software, and data analysis viewpoints for technology development within viral vector biomanufacturing.</p></div>","PeriodicalId":8946,"journal":{"name":"Biotechnology advances","volume":"74 ","pages":"Article 108391"},"PeriodicalIF":16.0,"publicationDate":"2024-06-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141287685","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nucleic acid-responsive smart systems for controlled cargo delivery","authors":"Akbar Hasanzadeh ,&nbsp;Arefeh Ebadati ,&nbsp;Sara Saeedi ,&nbsp;Babak Kamali ,&nbsp;Hamid Noori ,&nbsp;Behnam Jamei ,&nbsp;Michael R. Hamblin ,&nbsp;Yong Liu ,&nbsp;Mahdi Karimi","doi":"10.1016/j.biotechadv.2024.108393","DOIUrl":"10.1016/j.biotechadv.2024.108393","url":null,"abstract":"<div><p>Stimulus-responsive delivery systems allow controlled, highly regulated, and efficient delivery of various cargos while minimizing side effects. Owing to the unique properties of nucleic acids, including the ability to adopt complex structures by base pairing, their easy synthesis, high specificity, shape memory, and configurability, they have been employed in autonomous molecular motors, logic circuits, reconfigurable nanoplatforms, and catalytic amplifiers. Moreover, the development of nucleic acid (NA)-responsive intelligent delivery vehicles is a rapidly growing field. These vehicles have attracted much attention in recent years due to their programmable, controllable, and reversible properties. In this work, we review several types of NA-responsive controlled delivery vehicles based on locks and keys, including DNA/RNA-responsive, aptamer-responsive, and CRISPR-responsive, and summarize their advantages and limitations.</p></div>","PeriodicalId":8946,"journal":{"name":"Biotechnology advances","volume":"74 ","pages":"Article 108393"},"PeriodicalIF":16.0,"publicationDate":"2024-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141199169","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Toward biomanufacturing of next-generation bacterial nanocellulose (BNC)-based materials with tailored properties: A review on genetic engineering approaches","authors":"Dariela Núñez ,&nbsp;Patricio Oyarzún ,&nbsp;Sebastián González ,&nbsp;Irene Martínez","doi":"10.1016/j.biotechadv.2024.108390","DOIUrl":"10.1016/j.biotechadv.2024.108390","url":null,"abstract":"<div><p>Bacterial nanocellulose (BNC) is a biopolymer that is drawing significant attention for a wide range of applications thanks to its unique structure and excellent properties, such as high purity, mechanical strength, high water holding capacity and biocompatibility. Nevertheless, the biomanufacturing of BNC is hindered due to its low yield, the instability of microbial strains and cost limitations that prevent it from being mass-produced on a large scale. Various approaches have been developed to address these problems by genetically modifying strains and to produce BNC-based biomaterials with added value. These works are summarized and discussed in the present article, which include the overexpression and knockout of genes related and not related with the nanocellulose biosynthetic operon, the application of synthetic biology approaches and CRISPR/Cas techniques to modulate BNC biosynthesis. Further discussion is provided on functionalized BNC-based biomaterials with tailored properties that are incorporated <em>in-vivo</em> during its biosynthesis using genetically modified strains either in single or co-culture systems (<em>in-vivo</em> manufacturing). This novel strategy holds potential to open the road toward cost-effective production processes and to find novel applications in a variety of technology and industrial fields.</p></div>","PeriodicalId":8946,"journal":{"name":"Biotechnology advances","volume":"74 ","pages":"Article 108390"},"PeriodicalIF":16.0,"publicationDate":"2024-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141186173","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The bioproduction of astaxanthin: A comprehensive review on the microbial synthesis and downstream extraction","authors":"Dawei Zhou ,&nbsp;Zhengyue Fei ,&nbsp;Guannan Liu ,&nbsp;Yujia Jiang ,&nbsp;Wankui Jiang ,&nbsp;Carol Sze Ki Lin ,&nbsp;Wenming Zhang ,&nbsp;Fengxue Xin ,&nbsp;Min Jiang","doi":"10.1016/j.biotechadv.2024.108392","DOIUrl":"10.1016/j.biotechadv.2024.108392","url":null,"abstract":"<div><p>Astaxanthin is a valuable orange-red carotenoid with wide applications in agriculture, food, cosmetics, pharmaceuticals and nutraceuticals areas. At present, the biological synthesis of astaxanthin mainly relies on <em>Haematococcus pluvialis</em> and <em>Xanthophyllomyces dendrorhous</em>. With the rapid development of synthetic biology, more recombinant microbial hosts have been genetically constructed for astaxanthin production including <em>Escherichia coli</em>, <em>Saccharomyces cerevisiae</em> and <em>Yarrowia lipolytica</em>. As multiple genes (15) were involved in the astaxanthin synthesis, it is particularly important to adopt different strategies to balance the metabolic flow towards the astaxanthin synthesis. Furthermore, astaxanthin is a fat-soluble compound stored intracellularly, hence efficient extraction methods are also essential for the economical production of astaxanthin. Several efficient and green extraction methods of astaxanthin have been reported in recent years, including the superfluid extraction, ionic liquid extraction and microwave-assisted extraction. Accordingly, this review will comprehensively introduce the advances on the astaxanthin production and extraction by using different microbial hosts and strategies to improve the astaxanthin synthesis and extraction efficiency.</p></div>","PeriodicalId":8946,"journal":{"name":"Biotechnology advances","volume":"74 ","pages":"Article 108392"},"PeriodicalIF":16.0,"publicationDate":"2024-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141199116","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The type V effectors for CRISPR/Cas-mediated genome engineering in plants","authors":"Ruixiang Zhang ,&nbsp;Nan Chai ,&nbsp;Taoli Liu ,&nbsp;Zhiye Zheng ,&nbsp;Qiupeng Lin ,&nbsp;Xianrong Xie ,&nbsp;Jun Wen ,&nbsp;Zi Yang ,&nbsp;Yao-Guang Liu ,&nbsp;Qinlong Zhu","doi":"10.1016/j.biotechadv.2024.108382","DOIUrl":"10.1016/j.biotechadv.2024.108382","url":null,"abstract":"<div><p>A plethora of CRISPR effectors, such as Cas3, Cas9, and Cas12a, are commonly employed as gene editing tools. Among these, Cas12 effectors developed based on Class II type V proteins exhibit distinct characteristics compared to Class II type VI and type II effectors, such as their ability to generate non-allelic DNA double-strand breaks, their compact structures, and the presence of a single RuvC-like nuclease domain. Capitalizing on these advantages, Cas12 family proteins have been increasingly explored and utilized in recent years. However, the characteristics and applications of different subfamilies within the type V protein family have not been systematically summarized. In this review, we focus on the characteristics of type V effector (CRISPR/Cas12) proteins and the current methods used to discover new effector proteins. We also summarize recent modifications based on engineering of type V effectors. In addition, we introduce the applications of type V effectors for gene editing in animals and plants, including the development of base editors, tools for regulating gene expression, methods for gene targeting, and biosensors. We emphasize the prospects for development and application of CRISPR/Cas12 effectors with the goal of better utilizing toolkits based on this protein family for crop improvement and enhanced agricultural production.</p></div>","PeriodicalId":8946,"journal":{"name":"Biotechnology advances","volume":"74 ","pages":"Article 108382"},"PeriodicalIF":16.0,"publicationDate":"2024-05-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141157550","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The potential of Pavlovophyceae species as a source of valuable carotenoids and polyunsaturated fatty acids for human consumption","authors":"Filipe Maciel ,&nbsp;Leandro Madureira ,&nbsp;Pedro Geada ,&nbsp;José António Teixeira ,&nbsp;Joana Silva ,&nbsp;António Augusto Vicente","doi":"10.1016/j.biotechadv.2024.108381","DOIUrl":"10.1016/j.biotechadv.2024.108381","url":null,"abstract":"<div><p>Microalgae are a group of microorganisms, mostly photoautotrophs with high CO₂ fixation capacity, that have gained increased attention in the last decades due to their ability to produce a wide range of valuable metabolites, such as carotenoids and polyunsaturated fatty acids, for application in food/feed, pharmaceutical, and cosmeceutical industries. Their increasing relevance has highlighted the importance of identifying and culturing new bioactive-rich microalgae species, as well as of a thorough understanding of the growth conditions to optimize the biomass production and master the biochemical composition according to the desired application. Thus, this review intends to describe the main cell processes behind the production of carotenoids and polyunsaturated fatty acids, in order to understand the possible main triggers responsible for the accumulation of those biocompounds. Their economic value and the biological relevance for human consumption are also summarized. In addition, an extensive review of the impact of culture conditions on microalgae growth performance and their biochemical composition is presented, focusing mainly on the studies involving Pavlovophyceae species. A complementary description of the biochemical composition of these microalgae is also presented, highlighting their potential applications as a promising bioresource of compounds for large-scale production and human and animal consumption.</p></div>","PeriodicalId":8946,"journal":{"name":"Biotechnology advances","volume":"74 ","pages":"Article 108381"},"PeriodicalIF":16.0,"publicationDate":"2024-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0734975024000752/pdfft?md5=b1511b628897196870b06df8f89dd336&pid=1-s2.0-S0734975024000752-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141079466","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"ATP regeneration by ATPases for in vitro biotransformation","authors":"Lijing Chang ,&nbsp;Huijuan Cui ,&nbsp;Fei Li ,&nbsp;Yi-Heng P. Job Zhang ,&nbsp;Lingling Zhang","doi":"10.1016/j.biotechadv.2024.108377","DOIUrl":"10.1016/j.biotechadv.2024.108377","url":null,"abstract":"<div><p>Adenosine triphosphate (ATP) regeneration is a significant step in both living cells and <em>in vitro</em> biotransformation (<em>iv</em>BT). Rotary motor ATP synthases (ATPases), which regenerate ATP in living cells, have been widely assembled in biomimetic structures for <em>in vitro</em> ATP synthesis. In this review, we present a comprehensive overview of ATPases, including the working principle, orientation and distribution density properties of ATPases, as well as the assembly strategies and applications of ATPase-based ATP regeneration modules. The original sources of ATPases for <em>in vitro</em> ATP regeneration include chromatophores, chloroplasts, mitochondria, and inverted <em>Escherichia coli</em> (<em>E. coli</em>) vesicles, which are readily accessible but unstable. Although significant advances have been made in the assembly methods for ATPase-artificial membranes in recent decades, it remains challenging to replicate the high density and orientation of ATPases observed <em>in vivo</em> using <em>in vitro</em> assembly methods. The use of bioproton pumps or chemicals for constructing proton motive forces (PMF) enables the versatility and potential of ATPase-based ATP regeneration modules. Additionally, overall robustness can be achieved <em>via</em> membrane component selection, such as polymers offering great mechanical stability, or by constructing a solid supporting matrix through layer-by-layer assembly techniques. Finally, the prospects of ATPase-based ATP regeneration modules can be expected with the technological development of ATPases and artificial membranes.</p></div>","PeriodicalId":8946,"journal":{"name":"Biotechnology advances","volume":"73 ","pages":"Article 108377"},"PeriodicalIF":16.0,"publicationDate":"2024-05-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141037292","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evolving perspectives on lutein production from microalgae - A focus on productivity and heterotrophic culture","authors":"Cristobal Camarena-Bernard ,&nbsp;Victor Pozzobon","doi":"10.1016/j.biotechadv.2024.108375","DOIUrl":"10.1016/j.biotechadv.2024.108375","url":null,"abstract":"<div><p>Increased consumer awareness for healthier and more sustainable products has driven the search for naturally sourced compounds as substitutes for chemically synthesized counterparts. Research on pigments of natural origin, such as carotenoids, particularly lutein, has been increasing for over three decades. Lutein is recognized for its antioxidant and photoprotective activity. Its ability to cross the blood-brain barrier allows it to act at the eye and brain level and has been linked to benefits for vision, cognitive function and other conditions. While marigold flower is positioned as the only crop from which lutein is extracted from and commercialized, microalgae are proposed as an alternative with several advantages over this terrestrial crop. The main barrier to scaling up lutein production from microalgae to the commercial level is the low productivity compared to the high costs. This review explores strategies to enhance lutein production in microalgae by emphasizing the overall productivity over lutein content alone. Evaluation of how culture parameters, such as light quality, nitrogen sufficiency, temperature and even stress factors, affect lutein content and biomass development in batch phototrophic cultures was performed. Overall, the total lutein production remains low under this metabolic regime due to the low biomass productivity of photosynthetic batch cultures. For this reason, we describe findings on microalgal cultures grown under different metabolic regimes and culture protocols (fed-batch, pulse-feed, semi-batch, semi-continuous, continuous). After a careful literature examination, two-step heterotrophic or mixotrophic cultivation strategies are suggested to surpass the lutein productivity achieved in single-step photosynthetic cultures. Furthermore, this review highlights the urgent need to develop technical feasibility studies at a pilot scale for these cultivation strategies, which will strengthen the necessary techno-economic analyses to drive their commercial production.</p></div>","PeriodicalId":8946,"journal":{"name":"Biotechnology advances","volume":"73 ","pages":"Article 108375"},"PeriodicalIF":16.0,"publicationDate":"2024-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0734975024000697/pdfft?md5=e89ef3b24871edbf0fc7fe1710210504&pid=1-s2.0-S0734975024000697-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140955823","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}