Biotechnology advances最新文献_第8页

Chemicals and fuels from lipid-containing biomass: A comprehensive exploration 从含脂生物质中提取化学品和燃料：全面探索。

IF 12.1 1区工程技术

Biotechnology advances Pub Date : 2024-07-26 DOI: 10.1016/j.biotechadv.2024.108418

Jung-Hun Kim , Minyoung Kim , Gyeongnam Park , Eunji Kim , Hocheol Song , Sungyup Jung , Young-Kwon Park , Yiu Fai Tsang , Jechan Lee , Eilhann E. Kwon

{"title":"Chemicals and fuels from lipid-containing biomass: A comprehensive exploration","authors":"Jung-Hun Kim , Minyoung Kim , Gyeongnam Park , Eunji Kim , Hocheol Song , Sungyup Jung , Young-Kwon Park , Yiu Fai Tsang , Jechan Lee , Eilhann E. Kwon","doi":"10.1016/j.biotechadv.2024.108418","DOIUrl":"10.1016/j.biotechadv.2024.108418","url":null,"abstract":"<div><p>In response to address the climate crisis, there has been a growing focus on substituting conventional refinery-derived products with those derived from biorefineries. The utilization of lipids as primary materials or intermediates for the synthesis of chemicals and fuels, which are integral to the existing chemical and petrochemical industries, is a key step in this transition. This review provides a comprehensive overview of the production of sustainable chemicals (acids and alcohols), biopolymers, and fuels (including gasoline, kerosene, biodiesel, and heavy fuel oil) from lipids derived from terrestrial and algal biomass. The production of chemicals from lipids involves diverse methods, including polymerization, epoxidation, and separation/purification. Additionally, the transformation of lipids into biofuels can be achieved through processes such as catalytic cracking, hydroprocessing, and transesterification. This review also suggests future research directions that further advance the lipid valorization processes, including enhancement of catalyst durability at harsh conditions, development of deoxygenation process with low H<sub>2</sub> consumption, investigation of precise separation of target compounds, increase in lipid accumulation in algal biomass, and development of methods that utilize residues and byproducts generated during lipid extraction and conversion.</p></div>","PeriodicalId":8946,"journal":{"name":"Biotechnology advances","volume":"75 ","pages":"Article 108418"},"PeriodicalIF":12.1,"publicationDate":"2024-07-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141787240","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Production of pyrimidine nucleosides in microbial systems via metabolic engineering: Theoretical analysis research and prospects 通过代谢工程在微生物系统中生产嘧啶核苷：理论分析研究与前景。

IF 12.1 1区工程技术

Biotechnology advances Pub Date : 2024-07-23 DOI: 10.1016/j.biotechadv.2024.108419

Xiangjun Zhang , Pilian Niu , Huiyan Liu , Haitian Fang

{"title":"Production of pyrimidine nucleosides in microbial systems via metabolic engineering: Theoretical analysis research and prospects","authors":"Xiangjun Zhang , Pilian Niu , Huiyan Liu , Haitian Fang","doi":"10.1016/j.biotechadv.2024.108419","DOIUrl":"10.1016/j.biotechadv.2024.108419","url":null,"abstract":"<div><p>Pyrimidine nucleosides, as intermediate materials of significant commercial value, find extensive applications in the pharmaceutical industry. However, the current production of pyrimidine nucleosides largely relies on chemical synthesis, creating environmental problems that do not align with sustainable development goals. Recent progress in systemic metabolic engineering and synthetic biology has enabled the synthesis of natural products like pyrimidine nucleosides through microbial fermentation, offering a more sustainable alternative. Nevertheless, the intricate and tightly regulated biosynthetic pathways involved in the microbial production of pyrimidine nucleosides pose a formidable challenge. This study focuses on metabolic engineering and synthetic biology strategies aimed at enhancing pyrimidine nucleoside production. These strategies include gene modification, transcriptional regulation, metabolic flux analysis, cofactor balance optimization, and transporter engineering. Finally, this research highlights the challenges involved in the further development of pyrimidine nucleoside-producing strains and offers potential solutions in order to provide theoretical guidance for future research endeavors in this field.</p></div>","PeriodicalId":8946,"journal":{"name":"Biotechnology advances","volume":"75 ","pages":"Article 108419"},"PeriodicalIF":12.1,"publicationDate":"2024-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141756992","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Strategies to overcome the challenges of low or no expression of heterologous proteins in Escherichia coli 克服大肠杆菌中异源蛋白表达量低或无表达的挑战的策略。

IF 12.1 1区工程技术

Biotechnology advances Pub Date : 2024-07-20 DOI: 10.1016/j.biotechadv.2024.108417

Ruizhao Jiang , Shuting Yuan , Yilong Zhou , Yuwen Wei , Fulong Li , Miaomiao Wang , Bo Chen , Huimin Yu

引用次数: 0

Unraveling the intricacies of glycosaminoglycan biosynthesis: Decoding the molecular symphony in understanding complex polysaccharide assembly 揭开糖胺聚糖生物合成的神秘面纱：解码分子交响乐，了解复杂多糖的组装。

IF 12.1 1区工程技术

Biotechnology advances Pub Date : 2024-07-20 DOI: 10.1016/j.biotechadv.2024.108416

Zhi-Yuan Yao , Jin-Song Gong , Jia-Yu Jiang , Chang Su , Wen-Han Zhao , Zheng-Hong Xu , Jin-Song Shi

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GLYCOCINS: The sugar peppered antimicrobials 糖精：含糖的抗菌剂。

IF 12.1 1区工程技术

Biotechnology advances Pub Date : 2024-07-19 DOI: 10.1016/j.biotechadv.2024.108415

Shimona Ahlawat , Bhupendra Nath Shukla , Vaidhvi Singh , Yogita Sharma , Pravinkumar Choudhary , Alka Rao

{"title":"GLYCOCINS: The sugar peppered antimicrobials","authors":"Shimona Ahlawat , Bhupendra Nath Shukla , Vaidhvi Singh , Yogita Sharma , Pravinkumar Choudhary , Alka Rao","doi":"10.1016/j.biotechadv.2024.108415","DOIUrl":"10.1016/j.biotechadv.2024.108415","url":null,"abstract":"<div><p><u>Glyco</u>sylated bacterio<u>cins</u>, known as glycocins, were first discovered in 2011. These bioactive peptides are produced by bacteria to gain survival advantages. They exhibit diverse types of glycans and demonstrate varied antimicrobial activity. Currently, there are 13 experimentally known glycocins, with over 250 identified in silico across different bacterial phyla. Notably, glycocins are recognized for their glycan-mediated antimicrobial activity, proving effective against drug-resistant and foodborne pathogens. Many glycocins contain rare S-linked glycans. Glycosyltransferases (GTs), responsible for transferring sugar to glycocins and involved in glycocin biosynthesis, often cluster together in the producer's genome. This clustering makes them valuable for custom glycoengineering with diverse substrate specificities. Heterologous expression of glycocins has paved the way for the establishment of microbial factories for glycopeptide and glycoconjugate production across various industries. In this review, we emphasize the primary roles of fully and partially characterized glycocins and their glycosylating enzymes. Additionally, we explore how specific glycan structures facilitate these functions in antibacterial activities. Furthermore, we discuss newer approaches and increasing efforts aimed at exploiting bacterial glycobiology for the development of food preservatives and as replacements or complements to traditional antibiotics, particularly in the face of antibiotic-resistant pathogenic bacteria.</p></div>","PeriodicalId":8946,"journal":{"name":"Biotechnology advances","volume":"75 ","pages":"Article 108415"},"PeriodicalIF":12.1,"publicationDate":"2024-07-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141733500","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bacterial epigenetics and its implication for agriculture, probiotics development, and biotechnology design 细菌表观遗传学及其对农业、益生菌开发和生物技术设计的影响。

IF 12.1 1区工程技术

Biotechnology advances Pub Date : 2024-07-15 DOI: 10.1016/j.biotechadv.2024.108414

Tatyana L. Povolotsky , Hilit Levy Barazany , Yosi Shacham , Ilana Kolodkin-Gal

{"title":"Bacterial epigenetics and its implication for agriculture, probiotics development, and biotechnology design","authors":"Tatyana L. Povolotsky , Hilit Levy Barazany , Yosi Shacham , Ilana Kolodkin-Gal","doi":"10.1016/j.biotechadv.2024.108414","DOIUrl":"10.1016/j.biotechadv.2024.108414","url":null,"abstract":"<div><p>In their natural habitats, organisms encounter numerous external stimuli and must be able to sense and adapt to those stimuli to survive. Unlike mutations, epigenetic changes do not alter the underlying DNA sequence. Instead, they create modifications that promote or silence gene expression.</p><p><em>Bacillus subtilis</em> has long been a model organism in studying genetics and development. It is beneficial for numerous biotechnological applications where it is included as a probiotic, in fermentation, or in bio-concrete design. This bacterium has also emerged recently as a model organism for studying bacterial epigenetic adaptation. In this review, we examine the evolving knowledge of epigenetic regulation (restriction-modification systems (RM), orphan methyltransferases, and chromosome condensation) in <em>B. subtilis</em> and related bacteria, and utilize it as a case study to test their potential roles and future applications in genetic engineering and microbial biotechnology.</p><p>Finally, we suggest how the implementation of these fundamental findings promotes the design of synthetic epigenetic memory circuits and their future applications in agriculture, medicine, and biotechnology.</p></div>","PeriodicalId":8946,"journal":{"name":"Biotechnology advances","volume":"75 ","pages":"Article 108414"},"PeriodicalIF":12.1,"publicationDate":"2024-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141632521","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Engineering two-component systems for advanced biosensing: From architecture to applications in biotechnology 用于先进生物传感的双组分系统工程：从结构到生物技术应用。

IF 12.1 1区工程技术

Biotechnology advances Pub Date : 2024-07-11 DOI: 10.1016/j.biotechadv.2024.108404

Wenyan Cao, Chao Huang, Xuan Zhou, Shenghu Zhou, Yu Deng

引用次数: 0

An integrated perspective on measuring cytokines to inform CAR-T bioprocessing 以综合视角测量细胞因子，为 CAR-T 生物处理提供信息。

IF 12.1 1区工程技术

Biotechnology advances Pub Date : 2024-07-10 DOI: 10.1016/j.biotechadv.2024.108405

{"title":"An integrated perspective on measuring cytokines to inform CAR-T bioprocessing","authors":"","doi":"10.1016/j.biotechadv.2024.108405","DOIUrl":"10.1016/j.biotechadv.2024.108405","url":null,"abstract":"<div><p>Chimeric antigen receptor (CAR)-T cells are emerging as a generation-defining therapeutic however their manufacture remains a major barrier to meeting increased market demand. Monitoring critical quality attributes (CQAs) and critical process parameters (CPPs) during manufacture would vastly enrich acquired information related to the process and product, providing feedback to enable real-time decision making. Here we identify specific CAR-T cytokines as value-adding analytes and discuss their roles as plausible CPPs and CQAs. High sensitivity sensing technologies which can be easily integrated into manufacture workflows are essential to implement real-time monitoring of these cytokines. We therefore present biosensors as enabling technologies and evaluate recent advancements in cytokine detection in cell cultures, offering promising translatability to CAR-T biomanufacture. Finally, we outline emerging sensing technologies with future promise, and provide an overall outlook on existing gaps to implementation and the optimal sensing platform to enable cytokine monitoring in CAR-T biomanufacture.</p></div>","PeriodicalId":8946,"journal":{"name":"Biotechnology advances","volume":"75 ","pages":"Article 108405"},"PeriodicalIF":12.1,"publicationDate":"2024-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0734975024000995/pdfft?md5=60162a2ba3b6f87f3f7bb1a1987a48ae&pid=1-s2.0-S0734975024000995-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141598276","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Towards a seamless product and process development workflow for recombinant proteins produced by plant molecular farming 为植物分子农业生产的重组蛋白制定无缝的产品和工艺开发工作流程。

IF 12.1 1区工程技术

Biotechnology advances Pub Date : 2024-07-08 DOI: 10.1016/j.biotechadv.2024.108403

J.F. Buyel

{"title":"Towards a seamless product and process development workflow for recombinant proteins produced by plant molecular farming","authors":"J.F. Buyel","doi":"10.1016/j.biotechadv.2024.108403","DOIUrl":"10.1016/j.biotechadv.2024.108403","url":null,"abstract":"<div><p>Plant molecular farming (PMF) has been promoted as a fast, efficient and cost-effective alternative to bacteria and animal cells for the production of biopharmaceutical proteins. Numerous plant species have been tested to produce a wide range of drug candidates. However, PMF generally lacks a systematic, streamlined and seamless workflow to continuously fill the product pipeline. Therefore, it is currently unable to compete with established platforms in terms of routine, throughput and horizontal integration (the rapid translation of product candidates to preclinical and clinical development). Individual management decisions, limited funding and a lack of qualified production capacity can hinder the execution of such projects, but we also lack suitable technologies for sample handling and data management. This perspectives article will highlight current bottlenecks in PMF and offer potential solutions that combine PMF with existing technologies to build an integrated facility of the future for product development, testing, manufacturing and clinical translation. Ten major bottlenecks have been identified and are discussed in turn: automated cloning and simplified transformation options, reproducibility of bacterial cultivation, bioreactor integration with automated cell handling, options for rapid mid-scale candidate and product manufacturing, interconnection with (group-specific or personalized) clinical trials, diversity of (post-)infiltration conditions, development of downstream processing platforms, continuous process operation, compliance of manufacturing conditions with biosafety regulations, scaling requirements for cascading biomass.</p></div>","PeriodicalId":8946,"journal":{"name":"Biotechnology advances","volume":"75 ","pages":"Article 108403"},"PeriodicalIF":12.1,"publicationDate":"2024-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0734975024000971/pdfft?md5=aeaea65116ca81a42e627b82addc5923&pid=1-s2.0-S0734975024000971-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141578933","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The new frontier in CHO cell line development: From random to targeted transgene integration technologies CHO 细胞系开发的新领域：从随机到定向转基因整合技术。

IF 12.1 1区工程技术

Biotechnology advances Pub Date : 2024-06-29 DOI: 10.1016/j.biotechadv.2024.108402

{"title":"The new frontier in CHO cell line development: From random to targeted transgene integration technologies","authors":"","doi":"10.1016/j.biotechadv.2024.108402","DOIUrl":"10.1016/j.biotechadv.2024.108402","url":null,"abstract":"<div><p>Cell line development represents a crucial step in the development process of a therapeutic glycoprotein. Chinese hamster ovary (CHO) cells are the most frequently employed mammalian host cell system for the industrial manufacturing of biologics. The predominant application of CHO cells for heterologous recombinant protein expression lies in the relative simplicity of stably introducing ectopic DNA into the CHO host cell genome. Since CHO cells were first used as expression host for the industrial production of biologics in the late 1980s, stable genomic transgene integration has been achieved almost exclusively by random integration. Since then, random transgene integration had become the gold standard for generating stable CHO production cell lines due to a lack of viable alternatives. However, it was eventually demonstrated that this approach poses significant challenges on the cell line development process such as an increased risk of inducing cell line instability. In recent years, significant discoveries of new and highly potent (semi)-targeted transgene integration systems have paved the way for a technological revolution in the cell line development sector. These advanced methodologies comprise the application of transposase-, recombinase- or Cas9 nuclease-mediated site-specific genomic integration techniques, which enable a scarless transfer of the transgene expression cassette into transcriptionally active loci within the host cell genome. This review summarizes recent advancements in the field of transgene integration technologies for CHO cell line development and compare them to the established random integration approach. Moreover, advantages and limitations of (semi)-targeted integration techniques are discussed, and benefits and opportunities for the biopharmaceutical industry are outlined.</p></div>","PeriodicalId":8946,"journal":{"name":"Biotechnology advances","volume":"75 ","pages":"Article 108402"},"PeriodicalIF":12.1,"publicationDate":"2024-06-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S073497502400096X/pdfft?md5=11fd21ba09a2621d0568d8fa03e17d05&pid=1-s2.0-S073497502400096X-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141475825","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0