α酮酸脱羧酶：多样性，结构，反应机制，以及在平台化学品和燃料生物制造中的应用。

IF 12.1 1区工程技术 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biotechnology advances Pub Date : 2025-02-13 DOI:10.1016/j.biotechadv.2025.108531

Khanh Ha , Seunghyun Ryu , Cong T. Trinh

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引用次数: 0

摘要

在活细胞中，α酮酸脱羧酶（kdc, EC 4.1.1.-）是一类通过脱羧将α酮酸转化为醛的酶。这些醛可以作为生物合成醇、羧酸、酯和烷烃的化学试剂或前体。这些化合物在细胞代谢和适应性以及生物经济中起着至关重要的作用，促进了平台化学品和燃料的可持续和可再生生物制造。本文综述了kdc的多样性和分类，详细介绍了它们的结构、机制和功能。我们强调了最近在重新利用kdc以提高其生物制造效率和稳健性方面的进展。此外，我们提出模块化的kdc依赖性代谢途径，用于微生物合成醛、醇、羧酸、酯和烷烃。最后，我们讨论了模块化细胞工程技术的最新发展，该技术可以潜在地应用于利用生物制造平台化学品和燃料的kdc依赖途径的多样性。

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Alpha-ketoacid decarboxylases: Diversity, structures, reaction mechanisms, and applications for biomanufacturing of platform chemicals and fuels

In living cells, alpha-ketoacid decarboxylases (KDCs, EC 4.1.1.-) are a class of enzymes that convert alpha-ketoacids into aldehydes through decarboxylation. These aldehydes serve as either drop-in chemicals or precursors for the biosynthesis of alcohols, carboxylic acids, esters, and alkanes. These compounds play crucial roles in cellular metabolism and fitness and the bioeconomy, facilitating the sustainable and renewable biomanufacturing of platform chemicals and fuels. This review explores the diversity and classification of KDCs, detailing their structures, mechanisms, and functions. We highlight recent advancements in repurposing KDCs to enhance their efficiency and robustness for biomanufacturing. Additionally, we present modular KDC-dependent metabolic pathways for the microbial biosynthesis of aldehydes, alcohols, carboxylic acids, esters, and alkanes. Finally, we discuss recent developments in the modular cell engineering technology that can potentially be applied to harness the diversity of KDC-dependent pathways for biomanufacturing platform chemicals and fuels.

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来源期刊

Biotechnology advances 工程技术-生物工程与应用微生物

CiteScore

25.50

自引率

2.50%

发文量

167

审稿时长

37 days

期刊介绍： Biotechnology Advances is a comprehensive review journal that covers all aspects of the multidisciplinary field of biotechnology. The journal focuses on biotechnology principles and their applications in various industries, agriculture, medicine, environmental concerns, and regulatory issues. It publishes authoritative articles that highlight current developments and future trends in the field of biotechnology. The journal invites submissions of manuscripts that are relevant and appropriate. It targets a wide audience, including scientists, engineers, students, instructors, researchers, practitioners, managers, governments, and other stakeholders in the field. Additionally, special issues are published based on selected presentations from recent relevant conferences in collaboration with the organizations hosting those conferences.