Unleashing potential of microbial biosynthesis of monoterpenes via enzyme and metabolic engineering.

IF 12.1 1区工程技术 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biotechnology advances Pub Date : 2025-02-05 DOI:10.1016/j.biotechadv.2025.108525

Dianqi Yang, Hong Liang, Xuxu Li, Chenyu Zhang, Zewei Lu, Xiaoqiang Ma

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引用次数: 0

Abstract

Monoterpenes (MTPs) are valuable isoprenoids widely used in cosmetics, food flavorings, pharmaceuticals, etc. Compared to plant extraction and chemical synthesis, microbial biosynthesis offers superior sustainability and efficiency in producing natural MTPs, overcoming the limitations of raw material dependency, environmental impact, and racemic mixtures inherent in these methods. This review comprehensively discusses the development of natural or non-natural biosynthetic pathways for producing regular and irregular MTPs, emphasizing the importance of enzyme and metabolic engineering to optimize monoterpene synthases (MTPSs) in various engineered microbial cell factories (MCFs). The advances in functional expression of MTPS to enhance enzyme activity, substrate channeling of MTPS with critical biosynthesis enzymes, protein engineering of MTPS, targeted localization of MTPS in the subcellular organelle, and other favorable engineering strategies are discussed in detail. Leveraging these technologies, the engineered microbes will achieve the production of the defined product profile with higher titer/yield/productivity and improved industrial adaptability. Furthermore, we highlight the important development direction for optimizing MTPS performance and biosynthetic pathways, ensuring the microbial production of natural MTPs in a more efficient and application-specific manner.

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来源期刊

Biotechnology advances 工程技术-生物工程与应用微生物

CiteScore

25.50

自引率

2.50%

发文量

167

审稿时长

37 days

期刊介绍： Biotechnology Advances is a comprehensive review journal that covers all aspects of the multidisciplinary field of biotechnology. The journal focuses on biotechnology principles and their applications in various industries, agriculture, medicine, environmental concerns, and regulatory issues. It publishes authoritative articles that highlight current developments and future trends in the field of biotechnology. The journal invites submissions of manuscripts that are relevant and appropriate. It targets a wide audience, including scientists, engineers, students, instructors, researchers, practitioners, managers, governments, and other stakeholders in the field. Additionally, special issues are published based on selected presentations from recent relevant conferences in collaboration with the organizations hosting those conferences.