Recent advances in the application of CRISPR/Cas-based gene editing technology in Filamentous Fungi

IF 12.1 1区工程技术 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biotechnology advances Pub Date : 2025-03-12 DOI:10.1016/j.biotechadv.2025.108561

Xu-Hong Li, Hui-Zhi Lu, Ji-Bao Yao, Chi Zhang, Tian-Qiong Shi, He Huang

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引用次数: 0

Abstract

Filamentous fungi are essential industrial microorganisms that can serve as sources of enzymes, organic acids, terpenoids, and other bioactive compounds with significant applications in food, medicine, and agriculture. However, the underdevelopment of gene editing tools limits the full exploitation of filamentous fungi, which still present numerous untapped potential applications. In recent years, the CRISPR/Cas (clustered regularly interspaced short palindromic repeats) system, a versatile genome-editing tool, has advanced significantly and been widely applied in filamentous fungi, showcasing considerable research potential. This review examines the development and mechanisms of genome-editing tools in filamentous fungi, and contrasts the CRISPR/Cas9 and CRISPR/Cpf1 systems. The transformation and delivery strategies of the CRISPR/Cas system in filamentous fungi are also examined. Additionally, recent applications of CRISPR/Cas systems in filamentous fungi are summarized, such as gene disruption, base editing, and gene regulation. Strategies to enhance editing efficiency and reduce off-target effects are also highlighted, with the aim of providing insights for the future construction and optimization of CRISPR/Cas systems in filamentous fungi.

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基于CRISPR/ cas的基因编辑技术在丝状真菌中的应用进展

丝状真菌是必不可少的工业微生物，可以作为酶、有机酸、萜类化合物和其他生物活性化合物的来源，在食品、医药和农业中具有重要应用。然而，基因编辑工具的不发达限制了丝状真菌的充分利用，丝状真菌仍有许多未开发的潜在应用。近年来，CRISPR/Cas （clustered regularly interspaced short palindromic repeats）系统作为一种多用途的基因组编辑工具，在丝状真菌中取得了重大进展并得到了广泛应用，显示出相当大的研究潜力。本文综述了丝状真菌基因组编辑工具的发展和机制，并比较了CRISPR/Cas9和CRISPR/Cpf1系统。研究了丝状真菌中CRISPR/Cas系统的转化和传递策略。此外，综述了近年来CRISPR/Cas系统在丝状真菌中的应用，如基因破坏、碱基编辑和基因调控。重点介绍了提高编辑效率和减少脱靶效应的策略，旨在为丝状真菌CRISPR/Cas系统的未来构建和优化提供见解。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Biotechnology advances 工程技术-生物工程与应用微生物

CiteScore

25.50

自引率

2.50%

发文量

167

审稿时长

37 days

期刊介绍： Biotechnology Advances is a comprehensive review journal that covers all aspects of the multidisciplinary field of biotechnology. The journal focuses on biotechnology principles and their applications in various industries, agriculture, medicine, environmental concerns, and regulatory issues. It publishes authoritative articles that highlight current developments and future trends in the field of biotechnology. The journal invites submissions of manuscripts that are relevant and appropriate. It targets a wide audience, including scientists, engineers, students, instructors, researchers, practitioners, managers, governments, and other stakeholders in the field. Additionally, special issues are published based on selected presentations from recent relevant conferences in collaboration with the organizations hosting those conferences.